                         SEQUENCE LISTING

<110>  Nova Southeastern University
       The Forsyth Institute
 
<120>  Clade C HIV-1 Envelope (ENV) Trimer Immunogens, Compositions 
       including the Clade C HIV-1 Envelope (ENV) Trimer Immunogens, and
        Uses Thereof

<130>  7752-P22-065

<150>  US 63/168,077
<151>  2021-03-30

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1989
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Clade C HIV-1 1086.c ENV SOSIP trimer  DNA sequence

<400>  1
atggacgcta tgaaaagggg gctgtgctgt gtgctgctgc tgtgcggggc tgtgtttgtg       60

tcacccagtc aggaaatcca cgccagattc cggagaggag ctaggatgga gggctcgtgg      120

gtcacagttt actatggagt gcctgtgtgg aaagaagcaa aaactactct attctgtgca      180

tcagatgcta aagcatatga gaaagaagtg cataatgtct gggctacaca tgcctgtgtg      240

cccacagatc ccaacccaca agaaatggtt ttggcaaatg taacagaaaa ttttaacatg      300

tggaaaaatg atatggtaga gcagatgcat gaggatataa ttagtttgtg ggatgaaagc      360

ctgaagccat gtgtgaagtt gacgccactc tgtgtcactt taaattgtac aaatgttaaa      420

gggaatgaga gtgacaccag tgaagtaatg aaaaattgct ctttcaaggc aaccacggaa      480

ctaaaggata aaaaacataa ggtgcatgcg ctgttttata aacttgatgt agtaccactt      540

aatggaaaca gcagcagctc tggagagtat agattaataa attgcaatac ctcagccata      600

acacaagcct gtccaaaggt ctcttttgac ccaattcctt tacattactg tgcaccagct      660

ggttttgcga ttctaaagtg taataataag acattcaatg ggacaggacc atgtcgtaat      720

gtcagcacag tacaatgtac acatggaatt aagccagtgg tatcaactca actactgtta      780

aatggtagcc tagcagaaga agagataata attagatctg aaaatctgac aaacaatgcc      840

aaaacaataa tagtacacct caatgaatct gtaaacattg tgtgtacaag acccaataat      900

aatacaagaa aaagtataag gataggacca ggacaaacat tctatgcaac aggtgacata      960

ataggaaaca taagacaggc acattgtaac attaatgaaa gtaaatggaa caacacttta     1020

caaaaggtag gagaagaatt agcaaaacac ttccctagta aaacaataaa gtttgaacca     1080

tcctcaggag gcgatctaga aattacaaca catagcttta attgtagagg agagttcttc     1140

tattgcaata catcagacct gtttaatggt acatacagaa atggtacata caatcataca     1200

ggaagaagtt caaatggaac catcaccctc caatgcaaaa taaaacaaat tataaacatg     1260

tggcaggagg taggaagagc aatatatgcg cctcccattg aaggagaaat aacatgtaac     1320

tcaaatatca caggactact attgctacgt gatggaggtc aatcaaatga aacaaatgac     1380

acagagacat tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat     1440

aaatataaag tagtagaaat taaaccattg ggagtagcac ccactgagtg taaaagaaga     1500

gtggtggaga gacggagacg gagaagagca gtgggaatag gagctgtgtt ccttgggttc     1560

ttgggagcag ccggaagcac tatgggcgca gcatcaatga cgctgacggt acaggccagg     1620

caattattgt ctggtatagt gcaacagcaa agcaatttgc tgagggctcc agaggcgcaa     1680

cagcatatgt tgcaactcac ggtctggggc attaaacagc tccaggcaag agtcctggct     1740

atagaaagat acctaaagga tcaacagctc ctagggatgt ggggctgctc tggaaaactc     1800

atctgctgta ctgctgtgcc ttggaactcc agttggagta acaaatctca aaatgaaatt     1860

tggggcaaca tgacctggat gcagtgggac agagaaatta ataattacac aaacacaata     1920

tataggttac ttgaagactc acaaaaccag caggaaaaaa atgagaaaga tttgttagca     1980

ttggactaa                                                             1989


<210>  2
<211>  627
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clade C HIV-1 1086.c ENV SOSIP trimer protein sequence without 
       signal sequence

<400>  2

Met Glu Gly Ser Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Met Val Leu Ala Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Glu Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asn Val Lys Gly Asn Glu Ser Asp Thr Ser 
            100                 105                 110         


Glu Val Met Lys Asn Cys Ser Phe Lys Ala Thr Thr Glu Leu Lys Asp 
        115                 120                 125             


Lys Lys His Lys Val His Ala Leu Phe Tyr Lys Leu Asp Val Val Pro 
    130                 135                 140                 


Leu Asn Gly Asn Ser Ser Ser Ser Gly Glu Tyr Arg Leu Ile Asn Cys 
145                 150                 155                 160 


Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro 
                165                 170                 175     


Ile Pro Leu His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu Lys Cys 
            180                 185                 190         


Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn Val Ser Thr 
        195                 200                 205             


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
    210                 215                 220                 


Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn 
225                 230                 235                 240 


Leu Thr Asn Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val 
                245                 250                 255     


Asn Ile Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg 
            260                 265                 270         


Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asn 
        275                 280                 285             


Ile Arg Gln Ala His Cys Asn Ile Asn Glu Ser Lys Trp Asn Asn Thr 
    290                 295                 300                 


Leu Gln Lys Val Gly Glu Glu Leu Ala Lys His Phe Pro Ser Lys Thr 
305                 310                 315                 320 


Ile Lys Phe Glu Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
                325                 330                 335     


Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu 
            340                 345                 350         


Phe Asn Gly Thr Tyr Arg Asn Gly Thr Tyr Asn His Thr Gly Arg Ser 
        355                 360                 365             


Ser Asn Gly Thr Ile Thr Leu Gln Cys Lys Ile Lys Gln Ile Ile Asn 
    370                 375                 380                 


Met Trp Gln Glu Val Gly Arg Ala Ile Tyr Ala Pro Pro Ile Glu Gly 
385                 390                 395                 400 


Glu Ile Thr Cys Asn Ser Asn Ile Thr Gly Leu Leu Leu Leu Arg Asp 
                405                 410                 415     


Gly Gly Gln Ser Asn Glu Thr Asn Asp Thr Glu Thr Phe Arg Pro Gly 
            420                 425                 430         


Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
        435                 440                 445             


Val Val Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Glu Cys Lys Arg 
    450                 455                 460                 


Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala 
465                 470                 475                 480 


Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala 
                485                 490                 495     


Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val 
            500                 505                 510         


Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met 
        515                 520                 525             


Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
    530                 535                 540                 


Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Met Trp Gly 
545                 550                 555                 560 


Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Ser Ser 
                565                 570                 575     


Trp Ser Asn Lys Ser Gln Asn Glu Ile Trp Gly Asn Met Thr Trp Met 
            580                 585                 590         


Gln Trp Asp Arg Glu Ile Asn Asn Tyr Thr Asn Thr Ile Tyr Arg Leu 
        595                 600                 605             


Leu Glu Asp Ser Gln Asn Gln Gln Glu Lys Asn Glu Lys Asp Leu Leu 
    610                 615                 620                 


Ala Leu Asp 
625         


<210>  3
<211>  662
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clade C HIV-1 1086.c ENV SOSIP trimer protein sequence with 
       signal sequence

<400>  3

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg 
            20                  25                  30          


Gly Ala Arg Met Glu Gly Ser Trp Val Thr Val Tyr Tyr Gly Val Pro 
        35                  40                  45              


Val Trp Lys Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys 
    50                  55                  60                  


Ala Tyr Glu Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val 
65                  70                  75                  80  


Pro Thr Asp Pro Asn Pro Gln Glu Met Val Leu Ala Asn Val Thr Glu 
                85                  90                  95      


Asn Phe Asn Met Trp Lys Asn Asp Met Val Glu Gln Met His Glu Asp 
            100                 105                 110         


Ile Ile Ser Leu Trp Asp Glu Ser Leu Lys Pro Cys Val Lys Leu Thr 
        115                 120                 125             


Pro Leu Cys Val Thr Leu Asn Cys Thr Asn Val Lys Gly Asn Glu Ser 
    130                 135                 140                 


Asp Thr Ser Glu Val Met Lys Asn Cys Ser Phe Lys Ala Thr Thr Glu 
145                 150                 155                 160 


Leu Lys Asp Lys Lys His Lys Val His Ala Leu Phe Tyr Lys Leu Asp 
                165                 170                 175     


Val Val Pro Leu Asn Gly Asn Ser Ser Ser Ser Gly Glu Tyr Arg Leu 
            180                 185                 190         


Ile Asn Cys Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro Lys Val Ser 
        195                 200                 205             


Phe Asp Pro Ile Pro Leu His Tyr Cys Ala Pro Ala Gly Phe Ala Ile 
    210                 215                 220                 


Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn 
225                 230                 235                 240 


Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr 
                245                 250                 255     


Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg 
            260                 265                 270         


Ser Glu Asn Leu Thr Asn Asn Ala Lys Thr Ile Ile Val His Leu Asn 
        275                 280                 285             


Glu Ser Val Asn Ile Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys 
    290                 295                 300                 


Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile 
305                 310                 315                 320 


Ile Gly Asn Ile Arg Gln Ala His Cys Asn Ile Asn Glu Ser Lys Trp 
                325                 330                 335     


Asn Asn Thr Leu Gln Lys Val Gly Glu Glu Leu Ala Lys His Phe Pro 
            340                 345                 350         


Ser Lys Thr Ile Lys Phe Glu Pro Ser Ser Gly Gly Asp Leu Glu Ile 
        355                 360                 365             


Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr 
    370                 375                 380                 


Ser Asp Leu Phe Asn Gly Thr Tyr Arg Asn Gly Thr Tyr Asn His Thr 
385                 390                 395                 400 


Gly Arg Ser Ser Asn Gly Thr Ile Thr Leu Gln Cys Lys Ile Lys Gln 
                405                 410                 415     


Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Ile Tyr Ala Pro Pro 
            420                 425                 430         


Ile Glu Gly Glu Ile Thr Cys Asn Ser Asn Ile Thr Gly Leu Leu Leu 
        435                 440                 445             


Leu Arg Asp Gly Gly Gln Ser Asn Glu Thr Asn Asp Thr Glu Thr Phe 
    450                 455                 460                 


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 


Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Glu 
                485                 490                 495     


Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
    530                 535                 540                 


Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln 
545                 550                 555                 560 


Gln His Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Met Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp 
        595                 600                 605             


Asn Ser Ser Trp Ser Asn Lys Ser Gln Asn Glu Ile Trp Gly Asn Met 
    610                 615                 620                 


Thr Trp Met Gln Trp Asp Arg Glu Ile Asn Asn Tyr Thr Asn Thr Ile 
625                 630                 635                 640 


Tyr Arg Leu Leu Glu Asp Ser Gln Asn Gln Gln Glu Lys Asn Glu Lys 
                645                 650                 655     


Asp Leu Leu Ala Leu Asp 
            660         


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  4

Asp Met Arg Gly Glu Leu 
1               5       


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  5

Gln Gly Asn Arg Ser Asn Asn 
1               5           


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  6

Val Ser Lys Ala Thr 
1               5   


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  7

Trp Ile Ser Asn 
1               


<210>  8
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  8

Ser Val Gln Gly Ser 
1               5   


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505 SOSIP trimer protein fragment

<400>  9

Arg Asn Leu Ser 
1               


