                         SEQUENCE LISTING

<110>  Arizona Board of Regents on behalf of Arizona State 
       University
       Jacobs, Bertram
       Kibler, Karen
 
<120>  HIGHLY ATTENUATED REPLICATION-COMPETENT RECOMBINANT POXVIRUSES AS
        A VACCINE PLATFORM AND METHODS OF USE

<130>  112624.01328

<150>  63/168,140
<151>  2021-03-30

<150>  63/251,319
<151>  2021-10-01

<150>  63/286,961
<151>  2021-12-07

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  1273
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2


<220>
<221>  MISC_FEATURE
<222>  (1)..(1273)
<223>  Spike protein of SARS-Co-V2

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  2
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- C7L polypeptide

<400>  2

Met Gly Ile Gln His Glu Phe Asp Ile Ile Ile Asn Gly Asp Ile Ala 
1               5                   10                  15      


Leu Arg Asn Leu Gln Leu His Lys Gly Asp Asn Tyr Gly Cys Lys Leu 
            20                  25                  30          


Lys Ile Ile Ser Asn Asp Tyr Lys Lys Leu Lys Phe Arg Phe Ile Ile 
        35                  40                  45              


Arg Pro Asp Trp Ser Glu Ile Asp Glu Val Lys Gly Leu Thr Val Phe 
    50                  55                  60                  


Ala Asn Asn Tyr Ala Val Lys Val Asn Lys Val Asp Asp Thr Phe Tyr 
65                  70                  75                  80  


Tyr Val Ile Tyr Glu Ala Val Ile His Leu Tyr Asn Lys Lys Thr Glu 
                85                  90                  95      


Ile Leu Ile Tyr Ser Asp Asp Glu Asn Glu Leu Phe Lys His Tyr Tyr 
            100                 105                 110         


Pro Tyr Ile Ser Leu Asn Met Ile Ser Lys Lys Tyr Lys Val Lys Glu 
        115                 120                 125             


Glu Asn Tyr Ser Ser Pro Tyr Ile Glu His Pro Leu Ile Pro Tyr Arg 
    130                 135                 140                 


Asp Tyr Glu Ser Met Asp 
145                 150 


<210>  3
<211>  284
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- K1L polypeptide

<400>  3

Met Asp Leu Ser Arg Ile Asn Thr Trp Lys Ser Lys Gln Leu Lys Ser 
1               5                   10                  15      


Phe Leu Ser Ser Lys Asp Thr Phe Lys Ala Asp Val His Gly His Ser 
            20                  25                  30          


Ala Leu Tyr Tyr Ala Ile Ala Asp Asn Asn Val Arg Leu Val Cys Thr 
        35                  40                  45              


Leu Leu Asn Ala Gly Ala Leu Lys Asn Leu Leu Glu Asn Glu Phe Pro 
    50                  55                  60                  


Leu His Gln Ala Ala Thr Leu Glu Asp Thr Lys Ile Val Lys Ile Leu 
65                  70                  75                  80  


Leu Phe Ser Gly Met Asp Asp Ser Gln Phe Asp Asp Lys Gly Asn Thr 
                85                  90                  95      


Ala Leu Tyr Tyr Ala Val Asp Ser Gly Asn Met Gln Thr Val Lys Leu 
            100                 105                 110         


Phe Val Lys Lys Asn Trp Arg Leu Met Phe Tyr Gly Lys Thr Gly Trp 
        115                 120                 125             


Lys Thr Ser Phe Tyr His Ala Val Met Leu Asn Asp Val Ser Ile Val 
    130                 135                 140                 


Ser Tyr Phe Leu Ser Glu Ile Pro Ser Thr Phe Asp Leu Ala Ile Leu 
145                 150                 155                 160 


Leu Ser Cys Ile His Thr Thr Ile Lys Asn Gly His Val Asp Met Met 
                165                 170                 175     


Ile Leu Leu Leu Asp Tyr Met Thr Ser Thr Asn Thr Asn Asn Ser Leu 
            180                 185                 190         


Leu Phe Ile Pro Asp Ile Lys Leu Ala Ile Asp Asn Lys Asp Ile Glu 
        195                 200                 205             


Met Leu Gln Ala Leu Phe Lys Tyr Asp Ile Asn Ile Tyr Ser Val Asn 
    210                 215                 220                 


Leu Glu Asn Val Leu Leu Asp Asp Ala Glu Ile Thr Lys Met Ile Ile 
225                 230                 235                 240 


Glu Lys His Val Glu Tyr Lys Ser Asp Ser Tyr Thr Lys Asp Leu Asp 
                245                 250                 255     


Ile Val Lys Asn Asn Lys Leu Asp Glu Ile Ile Ser Lys Asn Lys Glu 
            260                 265                 270         


Leu Arg Leu Met Tyr Val Asn Cys Val Lys Lys Asn 
        275                 280                 


<210>  4
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TEE

<400>  4
aaaactgcta a                                                            11


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Viral Synthetic Late Promoter (SLP)

<400>  5
catattgaag agacagagtg atatat                                            26


<210>  6
<211>  193
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD)

<400>  6

Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
1               5                   10                  15      


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
            20                  25                  30          


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
        35                  40                  45              


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
    50                  55                  60                  


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
65                  70                  75                  80  


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
                85                  90                  95      


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
            100                 105                 110         


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
        115                 120                 125             


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
    130                 135                 140                 


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
145                 150                 155                 160 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
                165                 170                 175     


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
            180                 185                 190         


Val 
    


<210>  7
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- M protein of SARS-CoV-2

<400>  7

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      


Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          


Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              


Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  


Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  


Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      


Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         


Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             


Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 


Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 


Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     


Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         


Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             


Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         


<210>  8
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- E protein of SARS-Co-V-2

<400>  8

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      


Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          


Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              


Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  


Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  


<210>  9
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- N protein of SARS-CoV-2

<400>  9

Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 
1               5                   10                  15      


Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 
            20                  25                  30          


Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 
        35                  40                  45              


Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 
    50                  55                  60                  


Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 
65                  70                  75                  80  


Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 
                85                  90                  95      


Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr 
            100                 105                 110         


Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp 
        115                 120                 125             


Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp 
    130                 135                 140                 


His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln 
145                 150                 155                 160 


Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser 
                165                 170                 175     


Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn 
            180                 185                 190         


Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala 
        195                 200                 205             


Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu 
    210                 215                 220                 


Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln 
225                 230                 235                 240 


Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys 
                245                 250                 255     


Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln 
            260                 265                 270         


Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp 
        275                 280                 285             


Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile 
    290                 295                 300                 


Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile 
305                 310                 315                 320 


Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala 
                325                 330                 335     


Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 
            340                 345                 350         


Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 
        355                 360                 365             


Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 
    370                 375                 380                 


Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 
385                 390                 395                 400 


Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 
                405                 410                 415     


Thr Gln Ala 
            


<210>  10
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TEE

<400>  10
catattgaag agacagagtg atatataaaa ctgctaa                                37


<210>  11
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TEE

<400>  11
agaaccatat tgaagagaca gagtgatata taaaactgct aa                          42


<210>  12
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TEE

<400>  12
agaaccatat tgaagagaca gagtgatata taaaactgct aactcaagca gcacaagaat       60

taaatgaata ccaagaaaat acttggccag                                        90


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- ebola glycoprotein epitope 6D8 tag

<400>  13

Val Tyr Lys Leu Asp Ile Ser Glu Ala 
1               5                   


<210>  14
<211>  5098
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV2- spike flanked by TK recombination arms


<220>
<221>  misc_feature
<222>  (11)..(691)
<223>  TK right arm CpVV

<220>
<221>  misc_feature
<222>  (11)..(593)
<223>  Right arm TK

<220>
<221>  misc_feature
<222>  (715)..(756)
<223>  E/L Promoter

<220>
<221>  misc_feature
<222>  (768)..(4592)
<223>  Spike

<220>
<221>  misc_feature
<222>  (775)..(784)
<223>  Trx Term

<220>
<221>  misc_feature
<222>  (3040)..(3048)
<223>  Trx Term

<220>
<221>  misc_feature
<222>  (3888)..(3898)
<223>  Trx Term

<220>
<221>  misc_feature
<222>  (4092)..(4100)
<223>  Trx Term

<220>
<221>  misc_feature
<222>  (4591)..(5090)
<223>  Left arm TK

<400>  14
ggcgcgccct gaatatgaag gagcaaaagg ttgtaacatt ttattaccgt gtgggatata       60

aaagtccttg atccattgat ctggaaacgg gcatctccat ttaagactag atgccacggg      120

gtttaaaata ctaatcatga cattttgtag agcgtaatta cttagtaaat ccgccgtact      180

aggttcattt cctcctcgtt tggatcttac atcagaaatt aaaataatct tagaaggatg      240

cagttgtttt ttgatggatc gtagatattc ctcatcaacg aaccgagtca ctagagtcac      300

atcacgcaat ccatttagaa taggatcatg atggcgtccg tcaattagca tccatttgat      360

aatcattcct aaattataga aatgatctct caaataacgt atatgtgtac caggagccga      420

tcctatatac actacggtgg caccatctaa tataccgtgt cgctgtaact tactaagaaa      480

aaataattct cctagtaata gttttaactg tccttgatac ggcagttttt ttgcgacctc      540

atttgcactt tctggttcgt aatctaactc attatcaatt tcctcaaaat acataaacgg      600

tttatctaac gacacaacat ccatttttaa gtattatatt aaaatttaat caatgtttat      660

ttttagtttt ttagataaaa aatataatat tagttaatta ggcggccgcg tcgacaaaaa      720

ttgaaatttt attttttttt tttggaatat aaatagactc gaggccacca tgtttgtgtt      780

tcttgtttta ttgccactag tctctagtca gtgtgttaat cttacaacca gaactcaatt      840

accccctgca tacactaatt ctttcacacg tggtgtttat taccctgaca aagttttcag      900

atcctcagtt ttacattcaa ctcaggactt gttcttacct ttcttttcca atgttacttg      960

gttccatgct atacatgtct ctgggaccaa tggtactaag aggtttgata accctgtctt     1020

accatttaat gatggtgttt attttgcttc cactgagaag tctaacataa taagaggctg     1080

gatttttggt actactttag attcgaagac ccagtcctta ttaattgtta ataacgctac     1140

taatgttgtt attaaagtct gtgaatttca attttgtaat gatccatttt taggtgttta     1200

ttaccacaaa aacaacaaaa gttggatgga aagtgagttc agagtttatt ctagtgcgaa     1260

taattgcact tttgaatatg tctctcagcc ttttttaatg gaccttgaag gaaaacaggg     1320

taatttcaaa aatttaagag aatttgtgtt taagaatatt gatggttatt ttaaaatata     1380

ttctaagcac acgcctatta atttagtgag agatctccct cagggttttt cggctttaga     1440

accattggta gatttaccaa taggtattaa catcactaga tttcaaactt tattagcttt     1500

acatagaagt tatttaactc ctggtgattc ttcttcaggt tggacagctg gtgctgcagc     1560

ttattatgtg ggttatttac aacctagaac tttcttatta aaatataatg aaaatggaac     1620

cattacagat gctgtagact gtgcattaga ccctttatca gaaacaaagt gtacgttaaa     1680

atccttcact gtagaaaaag gaatctatca aacttctaac tttagagtcc aaccaacaga     1740

atctattgtt agatttccta atattacaaa cttatgccct tttggtgaag tttttaacgc     1800

caccagattt gcatctgttt atgcttggaa cagaaagaga atcagcaact gtgttgctga     1860

ttattctgtc ttatataatt ccgcatcatt ttccactttt aagtgttatg gagtgtctcc     1920

tactaaatta aatgatctct gctttactaa tgtctatgca gattcatttg taattagagg     1980

tgatgaagtc agacaaatcg ctccagggca aactggaaag attgctgatt ataattataa     2040

attaccagat gattttacag gctgcgttat agcttggaat tctaacaatt tagattctaa     2100

ggttggtggt aattataatt acttatatag attatttaga aagtctaatt taaaaccttt     2160

tgagagagat atttcaactg aaatctatca ggccggtagc acaccttgta atggtgttga     2220

aggttttaat tgttactttc ctttacaatc atatggtttc caacccacta atggtgttgg     2280

ttaccaacca tacagagtag tagtattatc ttttgaactt ttacatgcac cagcaactgt     2340

ttgtggacct aaaaagtcta ctaatttagt taaaaacaaa tgtgtcaatt tcaacttcaa     2400

tggtttaaca ggcacaggtg ttttaactga gtctaacaaa aagtttctgc ctttccaaca     2460

atttggcaga gacattgctg acactactga tgctgtcaga gatccacaga cattagagat     2520

tttagacatt acaccatgtt cttttggtgg tgtcagtgtt ataacaccag gaacaaatac     2580

ttctaaccag gttgctgttt tatatcagga tgttaactgc acagaagtcc ctgttgctat     2640

tcatgcagat caattaactc ctacttggag agtttattct acaggttcta atgtttttca     2700

aacaagagca ggctgtttaa taggggctga acatgtcaac aactcatatg agtgtgacat     2760

acccattggt gcaggtatat gcgctagtta tcagactcag actaattctc ctgggagcgc     2820

aagtagtgta gctagtcaat ccatcattgc ctacactatg tcattaggtg cagaaaattc     2880

agttgcttac tctaataact ctattgccat acccacaaat tttactatta gtgttaccac     2940

agaaatttta ccagtgtcta tgaccaagac atcagtagat tgtacaatgt acatttgtgg     3000

tgattcaact gaatgcagca atcttttgtt gcaatatggc agtttctgta cacaattaaa     3060

ccgtgcttta actggaatag ctgttgaaca agacaaaaac acccaagaag tttttgcaca     3120

agtcaaacaa atttacaaaa caccaccaat taaagatttt ggtggtttta atttttcaca     3180

aatattacca gatccatcaa aaccaagcaa gagatcacca attgaagatt tacttttcaa     3240

caaagtgaca ttagcagatg ctggcttcat caaacaatat ggtgattgct taggtgatat     3300

tgctgctaga gacttaattt gtgcacaaaa gtttaacggc ttaactgttt taccaccttt     3360

attaacagat gaaatgattg ctcaatacac ttctgcatta ttagcgggta caatcacttc     3420

tggttggacc tttggtgcag gtccggcatt acaaatacca tttccgatgc aaatggctta     3480

tagatttaat ggtattggag ttacacagaa tgttttatat gagaaccaaa aattaattgc     3540

caaccaattt aatagtgcta ttggcaaaat tcaagactca ttatcttcca caccaagtgc     3600

attaggaaaa ttacaagatg tggtcaacca aaatgcacaa gctttaaaca cgttagttaa     3660

acaattaagc tccaattttg gtgcaatttc aagtgtttta aatgatatct tatcaagatt     3720

agacccacca gaggctgaag tgcaaattga tagattaatc acaggcagat tacaaagttt     3780

acagacatat gtgactcaac aattaattag agctgcagaa atcagagctt ctgctaattt     3840

agctgctact aaaatgtcag agtgtgtatt aggacaatca aaaagagttg atttctgtgg     3900

aaagggctat catttaatgt ccttccctca gtcagcacct catggtgtag tcttcttaca     3960

tgtgacttat gtccctgcac aagaaaagaa cttcacaact gctcctgcca tttgtcatga     4020

tggaaaagca cactttccta gagaaggtgt ctttgtttca aatggcacac actggtttgt     4080

aacacaaaga aatttctatg aaccacaaat cattactaca gacaacacat ttgtgtctgg     4140

taactgtgat gttgtaatag gaattgtcaa caacacagtt tatgatcctt tacaacctga     4200

attagactca ttcaaggagg agttagataa atattttaag aatcatacat caccagatgt     4260

tgatttaggt gacatctctg gcattaatgc ttcagttgta aacattcaaa aagaaattga     4320

cagattaaat gaggttgcca agaatttaaa tgaatcttta atcgatttac aagaattagg     4380

aaagtatgag cagtatataa aatggccatg gtacatttgg ttaggtttta tagctggctt     4440

aattgccata gtaatggtga caattatgtt atgctgtatg accagttgct gtagttgttt     4500

aaagggctgt tgttcttgtg gatcctgctg caaatttgat gaagacgact ctgagccagt     4560

gttaaaagga gtcaaattac attacacata accgtcagta tcggcggaat tacagctgag     4620

cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taatttttct cccgggtagc     4680

tagttaatta catgatgaca ataaagaatt aattattgtt cactttattc gactttaata     4740

tatccatcac gttagaaaat gcgatatcgc gacgaggatc tatgtatcta ataggatcta     4800

ttgcggtggt agctagagag gattcttttt tgaatcgcat caaactaatc acaaagtcga     4860

acaaatatcc tttattaagt ttgacccttc catctgtaac aatagggacc ttgttaaaca     4920

gttttttaaa atcttgagag tctgtgaatt ttgtcaattg tctgtattcc tctgaaagag     4980

attcataaca atgacccacg gcttctaatt tattttttga ttggatcaat aataataaca     5040

gaaagtctag atattgagtg atttgcaata tatcagataa tgaagattca cctcgagg       5098


<210>  15
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- modified S protein SARS-CoV-2


<220>
<221>  MISC_FEATURE
<222>  (682)..(685)
<223>  furin cleavage site

<220>
<221>  MISC_FEATURE
<222>  (817)..(817)
<223>  F817P

<220>
<221>  MISC_FEATURE
<222>  (892)..(892)
<223>  A892P

<220>
<221>  MISC_FEATURE
<222>  (899)..(899)
<223>  A899P

<220>
<221>  MISC_FEATURE
<222>  (942)..(942)
<223>  A942P

<220>
<221>  MISC_FEATURE
<222>  (986)..(986)
<223>  K986P

<220>
<221>  MISC_FEATURE
<222>  (987)..(987)
<223>  V987P

<400>  15

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  16
<211>  5098
<212>  DNA
<213>  Severe acute respiratory syndrome coronavirus 2


<220>
<221>  misc_feature
<222>  (1)..(5098)
<223>  SARS-CoV-2 Spike sequence

<400>  16
ggcgcgccct gaatatgaag gagcaaaagg ttgtaacatt ttattaccgt gtgggatata       60

aaagtccttg atccattgat ctggaaacgg gcatctccat ttaagactag atgccacggg      120

gtttaaaata ctaatcatga cattttgtag agcgtaatta cttagtaaat ccgccgtact      180

aggttcattt cctcctcgtt tggatcttac atcagaaatt aaaataatct tagaaggatg      240

cagttgtttt ttgatggatc gtagatattc ctcatcaacg aaccgagtca ctagagtcac      300

atcacgcaat ccatttagaa taggatcatg atggcgtccg tcaattagca tccatttgat      360

aatcattcct aaattataga aatgatctct caaataacgt atatgtgtac caggagccga      420

tcctatatac actacggtgg caccatctaa tataccgtgt cgctgtaact tactaagaaa      480

aaataattct cctagtaata gttttaactg tccttgatac ggcagttttt ttgcgacctc      540

atttgcactt tctggttcgt aatctaactc attatcaatt tcctcaaaat acataaacgg      600

tttatctaac gacacaacat ccatttttaa gtattatatt aaaatttaat caatgtttat      660

ttttagtttt ttagataaaa aatataatat tagttaatta ggcggccgcg tcgacaaaaa      720

ttgaaatttt attttttttt tttggaatat aaatagactc gaggccacca tgtttgtgtt      780

tcttgtttta ttaccattag tctctagtca gtgtgttaat ttaacaacca gaactcaatt      840

accccctgca tacactaatt ctttcacaag aggtgtttat taccctgaca aagttttcag      900

atcctcagtt ttacattcaa ctcaggactt attcttacct ttcttttcca atgttacttg      960

gttccatgct atacatgtct ctgggaccaa tggtactaag aggtttgata accctgtctt     1020

accatttaat gatggtgttt attttgcttc cactgagaag tctaacataa taagaggctg     1080

gatttttggt actactttag attcgaagac ccagtcctta ttaattgtta ataacgctac     1140

taatgttgtt attaaagtct gtgaatttca attttgtaat gatccatttt taggtgttta     1200

ttaccacaaa aacaacaaaa gttggatgga aagtgagttc agagtttatt ctagtgcgaa     1260

taattgcact tttgaatatg tctctcagcc ttttttaatg gaccttgaag gaaaacaggg     1320

taatttcaaa aatttaagag aatttgtgtt taagaatatt gatggttatt ttaaaatata     1380

ttctaagcac acgcctatta atttagtgag agatctccct cagggttttt cggctttaga     1440

accattggta gatttaccaa taggtattaa catcactaga tttcaaactt tattagcttt     1500

acatagaagt tatttaactc ctggtgattc ttcttcaggt tggacagctg gtgctgcagc     1560

ttattatgtg ggttatttac aacctagaac tttcttatta aaatataatg aaaatggaac     1620

cattacagat gctgtagact gtgcattaga ccctttatca gaaacaaagt gtacgttaaa     1680

atccttcact gtagaaaaag gaatctatca aacttctaac tttagagtcc aaccaacaga     1740

atctattgtt agatttccta atattacaaa cttatgccct tttggtgaag tttttaacgc     1800

caccagattt gcatctgttt atgcttggaa cagaaagaga atcagcaact gtgttgctga     1860

ttattctgtc ttatataatt ccgcatcatt ttccactttt aagtgttatg gagtgtctcc     1920

tactaaatta aatgatctct gctttactaa tgtctatgca gattcatttg taattagagg     1980

tgatgaagtc agacaaatcg ctccagggca aactggaaag attgctgatt ataattataa     2040

attaccagat gattttacag gctgcgttat agcttggaat tctaacaatt tagattctaa     2100

ggttggtggt aattataatt acttatatag attatttaga aagtctaatt taaaaccttt     2160

tgagagagat atttcaactg aaatctatca ggccggtagc acaccttgta atggtgttga     2220

aggttttaat tgttactttc ctttacaatc atatggtttc caacccacta atggtgttgg     2280

ttaccaacca tacagagtag tagtattatc ttttgaactt ttacatgcac cagcaactgt     2340

ttgtggacct aaaaagtcta ctaatttagt taaaaacaaa tgtgtcaatt tcaacttcaa     2400

tggtttaaca ggcacaggtg ttttaactga gtctaacaaa aagtttctgc ctttccaaca     2460

atttggcaga gacattgctg acactactga tgctgtcaga gatccacaga cattagagat     2520

tttagacatt acaccatgtt cttttggtgg tgtcagtgtt ataacaccag gaacaaatac     2580

ttctaaccag gttgctgttt tatatcagga tgttaactgc acagaagtcc ctgttgctat     2640

tcatgcagat caattaactc ctacttggag agtttattct acaggttcta atgtttttca     2700

aacaagagca ggctgtttaa taggggctga acatgtcaac aactcatatg agtgtgacat     2760

acccattggt gcaggtatat gcgctagtta tcagactcag actaattctc ctgggagcgc     2820

aagtagtgta gctagtcaat ccatcattgc ctacactatg tcattaggtg cagaaaattc     2880

agttgcttac tctaataact ctattgccat acccacaaat tttactatta gtgttaccac     2940

agaaatttta ccagtgtcta tgaccaagac atcagtagat tgtacaatgt acatttgtgg     3000

tgattcaact gaatgcagca atcttttgtt gcaatatggc agtttctgta cacaattaaa     3060

ccgtgcttta actggaatag ctgttgaaca agacaaaaac acccaagaag tttttgcaca     3120

agtcaaacaa atttacaaaa caccaccaat taaagatttt ggtggtttta atttttcaca     3180

aatattacca gatccatcaa aaccaagcaa gagatcacca attgaagatt tacttttcaa     3240

caaagtgaca ttagcagatg ctggcttcat caaacaatat ggtgattgct taggtgatat     3300

tgctgctaga gacttaattt gtgcacaaaa gtttaacggc ttaactgttt taccaccttt     3360

attaacagat gaaatgattg ctcaatacac ttctgcatta ttagcgggta caatcacttc     3420

tggttggacc tttggtgcag gtccggcatt acaaatacca tttccgatgc aaatggctta     3480

tagatttaat ggtattggag ttacacagaa tgttttatat gagaaccaaa aattaattgc     3540

caaccaattt aatagtgcta ttggcaaaat tcaagactca ttatcttcca caccaagtgc     3600

attaggaaaa ttacaagatg tggtcaacca aaatgcacaa gctttaaaca cgttagttaa     3660

acaattaagc tccaattttg gtgcaatttc aagtgtttta aatgatatct tatcaagatt     3720

agacccacca gaggctgaag tgcaaattga tagattaatc acaggcagat tacaaagttt     3780

acagacatat gtgactcaac aattaattag agctgcagaa atcagagctt ctgctaattt     3840

agctgctact aaaatgtcag agtgtgtatt aggacaatca aaaagagttg atttctgtgg     3900

aaagggctat catttaatgt ccttccctca gtcagcacct catggtgtag tcttcttaca     3960

tgtgacttat gtccctgcac aagaaaagaa cttcacaact gctcctgcca tttgtcatga     4020

tggaaaagca cactttccta gagaaggtgt ctttgtttca aatggcacac actggtttgt     4080

aacacaaaga aatttctatg aaccacaaat cattactaca gacaacacat ttgtgtctgg     4140

taactgtgat gttgtaatag gaattgtcaa caacacagtt tatgatcctt tacaacctga     4200

attagactca ttcaaggagg agttagataa atattttaag aatcatacat caccagatgt     4260

tgatttaggt gacatctctg gcattaatgc ttcagttgta aacattcaaa aagaaattga     4320

cagattaaat gaggttgcca agaatttaaa tgaatcttta atcgatttac aagaattagg     4380

aaagtatgag cagtatataa aatggccatg gtacatttgg ttaggtttta tagctggctt     4440

aattgccata gtaatggtga caattatgtt atgctgtatg accagttgct gtagttgttt     4500

aaagggctgt tgttcttgtg gatcctgctg caaatttgat gaagacgact ctgagccagt     4560

gttaaaagga gtcaaattac attacacata accgtcagta tcggcggaat tacagctgag     4620

cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taatttttct cccgggtagc     4680

tagttaatta catgatgaca ataaagaatt aattattgtt cactttattc gactttaata     4740

tatccatcac gttagaaaat gcgatatcgc gacgaggatc tatgtatcta ataggatcta     4800

ttgcggtggt agctagagag gattcttttt tgaatcgcat caaactaatc acaaagtcga     4860

acaaatatcc tttattaagt ttgacccttc catctgtaac aatagggacc ttgttaaaca     4920

gttttttaaa atcttgagag tctgtgaatt ttgtcaattg tctgtattcc tctgaaagag     4980

attcataaca atgacccacg gcttctaatt tattttttga ttggatcaat aataataaca     5040

gaaagtctag atattgagtg atttgcaata tatcagataa tgaagattca cctcgagg       5098


<210>  17
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- pfs-spike


<220>
<221>  MISC_FEATURE
<222>  (682)..(685)
<223>  rrar mutation furin cleavage site

<220>
<221>  MISC_FEATURE
<222>  (817)..(817)
<223>  F817P

<220>
<221>  MISC_FEATURE
<222>  (892)..(892)
<223>  A892P

<220>
<221>  MISC_FEATURE
<222>  (899)..(899)
<223>  A899P

<220>
<221>  MISC_FEATURE
<222>  (942)..(942)
<223>  A942P

<220>
<221>  MISC_FEATURE
<222>  (986)..(986)
<223>  K986P

<220>
<221>  MISC_FEATURE
<222>  (987)..(987)
<223>  V987P

<400>  17

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  18
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) G339D


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  G339D mutation

<400>  18

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Asp Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  19
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) S373P


<220>
<221>  MISC_FEATURE
<222>  (51)..(51)
<223>  S371L mutation

<400>  19

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Leu Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  20
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) S373P


<220>
<221>  MISC_FEATURE
<222>  (53)..(53)
<223>  S373P mutation

<400>  20

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Pro Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  21
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) S375F


<220>
<221>  MISC_FEATURE
<222>  (55)..(55)
<223>  S375F mutation

<400>  21

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Phe Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  22
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) K417N


<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  K417N mutation

<400>  22

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  23
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) K417T


<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  K417N mutation

<400>  23

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  24
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) K417M


<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  K417M mutation

<400>  24

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Met Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  25
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) N440K


<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  N440K mutation

<400>  25

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Lys Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  26
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic-  SARS-CoV-2 receptor binding domain (RBD) G446S


<220>
<221>  MISC_FEATURE
<222>  (126)..(126)
<223>  G446S mutation

<400>  26

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Ser Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  27
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) L452R


<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  L542R mutation

<400>  27

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  28
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) S477N


<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  S477N mutation

<400>  28

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Asn Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  29
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) T478K


<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  T478K mutation

<400>  29

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  30
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) E484K


<220>
<221>  MISC_FEATURE
<222>  (164)..(164)
<223>  E484K mutation

<400>  30

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  31
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) E484A


<220>
<221>  MISC_FEATURE
<222>  (164)..(164)
<223>  E484A mutation

<400>  31

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Ala Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  32
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) Q493K


<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Q493K mutation

<400>  32

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Lys Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  33
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) Q493R


<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Q493R mutation

<400>  33

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Arg Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  34
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) G496S


<220>
<221>  MISC_FEATURE
<222>  (176)..(176)
<223>  G496S mutation

<400>  34

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Ser 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  35
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) Q498R


<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Q498R mutation

<400>  35

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Arg Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  36
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) N501Y


<220>
<221>  MISC_FEATURE
<222>  (181)..(181)
<223>  N501Y mutation

<400>  36

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190 


<210>  37
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- SARS-CoV-2 receptor binding domain (RBD) Y505H


<220>
<221>  MISC_FEATURE
<222>  (185)..(185)
<223>  Y505H mutation

<400>  37

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
1               5                   10                  15      


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            20                  25                  30          


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        35                  40                  45              


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    50                  55                  60                  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
65                  70                  75                  80  


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                85                  90                  95      


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            100                 105                 110         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        115                 120                 125             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    130                 135                 140                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
145                 150                 155                 160 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                165                 170                 175     


Phe Gln Pro Thr Asn Gly Val Gly His Gln Pro Tyr Arg Val 
            180                 185                 190 


