                         SEQUENCE LISTING

<110>  California Institute of Technology
       Abedi, Mohamad H.
       Shapiro, Mikhail G.
       Yao, Michael S.
 
<120>  ACOUSTIC REMOTE CONTROL OF MICROBIAL IMMUNOTHERAPY

<130>  30KJ-302441-WO

<150>  63/160,152
<151>  2021-03-12

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  8296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha-PD-L1 Thermal Switching Circuit

<400>  1
gaaacagcct ctacaaataa ttttgtttaa gggcccaagt tcacgagaac gatatagaaa       60

gttccaggag aaaggtacca tgaaatacct gctgccgacc gctgctgctg gtctgctgct      120

cctcgctgcc cagccggcga tggccgctca agtccagtta gttgaaaccg gagggggatt      180

agttcaacct ggtggatcat tacgcttgtc ctgtacggct tcaggtttca ccttcagcat      240

gcatgcgatg acttggtatc gtcaggctcc cggaaagcaa cgcgagcttg tcgcagtaat      300

tacttcccat ggggatcgcg ccaactatac ggactctgtc cgtggccgct tcacaatctc      360

gcgtgataat actaaaaata tggtttactt gcagatgaac tctttaaaac cagaagatac      420

ggcggtttat tattgtaatg taccgcgcta tgattcttgg ggacagggta cgcaagtcac      480

ggtcagcagt ggtggcttac cagagacggg aggacatcat caccatcatc actaggtaag      540

gcaagctcgt actgggagtt ctaatgcgta aaggcgaaga gctgttcact ggtgtcgtcc      600

ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg      660

gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac      720

tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc      780

gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg      840

tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga      900

aatttgaagg cgatactctg gtaaaccgca ttgagctgaa aggcattgac tttaaagaag      960

acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca     1020

ccgccgataa acaaaaaaat ggcactaaag cgaattttaa aattcgccac aacgtggagg     1080

atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg     1140

ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg     1200

agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta     1260

tggatgaact gtacaaatga tgaactgggc acaacagagt aataattcaa aagagatgtc     1320

ggtttaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgccc     1380

caatgataac cccaagggaa gttttagtca aaagcctccg gatgtcactc aatgaagtcg     1440

cagggttgag gcgatgaggg gtccttccag gcatcaaata aaacgaaagg ctcagtcgaa     1500

agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa     1560

tccgccgccc tagacttagg cgttcggctg cggcgagcgg tatcagctca ctcaaagaat     1620

ccttagcttt cgctgaggat gatttctgct agaggcagag gtggttgtgt tgcgatcgag     1680

tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt     1740

gataatggtt gcatgtacta aggaggttgt atggaacaac gcataaccct gaaagattat     1800

gcaatgcgct ttgggcaaac caagacagct aaaagatctc tcacctacca aacaatgccc     1860

ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg tgataaatta     1920

tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc agcaggacgc     1980

actgaccacc atgaaggtga cgctcatgac ttacttgctg aatctcagga gtttatgacc     2040

tttttttttt agatcctatc ggtatgagag ccctggtagt catccgcctg tcccgcgtca     2100

ccgatgctac gacttcaccg gagcgtcagc tggagtcttg ccagcagctc tgcgcccagc     2160

gcggctggga cgtcgtcggg gtagcggagg atctggacgt ctccggggcg gtcgatccgt     2220

tcgaccggaa gcgcagaccg aacctggccc ggtggctagc gttcgaggag caaccgtttg     2280

acgtgatcgt ggcgtaccgg gtagatcggt tgacccgatc gatccggcat cttcagcagc     2340

tggtccactg ggccgaggac cacaagaagc tggtcgtctc cgcgaccgaa gcgcacttcg     2400

atacgacgac gccgtttgcg gcggtcgtca tcgcgcttat gggaacggtg gcgcagatgg     2460

aattagaagc gatcaaagag cggaaccgtt cggctgcgca tttcaatatc cgcgccggga     2520

aataccgagg atccctgccg ccgtggggat acctgcctac gcgcgtggac ggggagtggc     2580

ggctggtgcc ggaccctgtg cagcgagagc gcatcctcga ggtgtatcac cgcgtcgtcg     2640

acaaccacga gccgctgcat ctggtggccc acgacctgaa ccggcgtggt gtcctgtcgc     2700

cgaaggacta cttcgcgcag ctgcaaggcc gcgagccgca gggccgggag tggtcggcta     2760

ccgcgctgaa gcgatcgatg atctccgagg cgatgctcgg gtacgcgact ctgaacggta     2820

agaccgtccg agacgacgac ggagccccgc tggtgcgggc tgagccgatc ctgacccgtg     2880

agcagctgga ggcgctgcgc gccgagctcg tgaagacctc ccgggcgaag cccgcggtgt     2940

ctaccccgtc gctgctgctg cgggtgttgt tctgcgcggt gtgcggggag cccgcgtaca     3000

agttcgccgg gggaggacgt aagcacccgc gctaccgctg ccgctcgatg gggttcccga     3060

agcactgcgg gaacggcacg gtggcgatgg ccgagtggga cgcgttctgc gaggagcagg     3120

tactggatct gctcggggac gcggagcgtc tggagaaagt ctgggtagcg ggctcggact     3180

ccgcggtcga actcgcggag gtgaacgcgg agctggtgga cctgacgtcg ctgatcggct     3240

ccccggccta ccgggcgggc tctccgcagc gagaagcact ggatgcccgt attgcggcgc     3300

tggccgcgcg gcaagaggag ctggagggcc tggaggctcg cccgtctggc tgggagtggc     3360

gcgagaccgg gcagcggttc ggggactggt ggcgggagca ggacaccgcg gcaaagaaca     3420

cctggcttcg gtcgatgaac gttcggctga cgttcgacgt ccgcggcggg ctgactcgca     3480

cgatcgactt cggggatctt caggagtacg agcagcatct caggctcggc agcgtggtcg     3540

aacggctaca caccgggatg tcgggttcag cagcaaacga cgaaaactac gctgcacacc     3600

gatgatgctt aagtcgaaca gaaagtaatc gtatgagctc gacaccatcg aatggcgcaa     3660

aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt     3720

ataagaagga gatatacata tgagcacaaa aaagaatcca ttaacacaag agcagcttga     3780

ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttaaccca     3840

ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctc tatttaatgg     3900

catcaatgca ttaaatgctc ataacgccgc attgcttaca aaaattctca aagttagcgt     3960

tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat     4020

gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat     4080

gttctcacct gagcctagaa cctgtaccaa aggtgatgcg gagagatggg taagcacaac     4140

caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc     4200

aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc     4260

tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa     4320

actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat     4380

cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga     4440

gacgtttggc tgactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca     4500

catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata     4560

actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg     4620

aactatatcc ggattggctt cctgagtgat accttaccct gcctatttgc cctctcccag     4680

ggttatttcg atgaaaggag agagtactaa tgccaattgc aataggaaat aaacggttac     4740

ccgttacgtt ggatgaaaag agacaaaaag aattgcagca actaaagcag aagtacggca     4800

aaagtgaatc caggattatg tgtattgcgt tagatttatt gattgcccaa gaaaaagcag     4860

gatttgaggt accagcactc aaaaagtgac gtcacttttt atcctaaaaa ctaaaagtga     4920

tagcactttt aattataaga agttagaata ttaatcattt gcttaattgt acaatataat     4980

gtacaattgt tttatagaaa taaataaggg gtgaaaggaa tggaagcagt agcttattca     5040

aatttccgcc aaaatttacg tagttatatg aaacaagtta atgaggatgc tgaaacactt     5100

attgtaacaa gtaaagatgt agaagataca gttgttgtat tatcaaaaag agattatgat     5160

tctatgcaag aaacgttgag aacactttct aataattacg tcatggaaaa aattcgtcga     5220

ggagatgaac aattctccaa aggtgcattt aaaacacatg acttaatcga ggttgaatct     5280

gatgattaag gcttggtctg atgatgcttg ggatgattat ctttattggc atgagcaagg     5340

aaacaaaagc aatataaaaa agattaacaa gttaataaaa gatatcgatc gttccccctt     5400

tgctggatta ggaaaacctg agccattaaa gcatgattta tctggaaaat ggtccagaag     5460

aattacagat gaacatagac tgatatatag agttgaaaat gaaacgatat ttatttattc     5520

tgcaaaagat cactattaac caatcggaag taaggaaagg gtcagaaact taaaagtttt     5580

tgatccttat tttatttacc ctagtcattt aaaaagctaa tatagcttag tgttgattgt     5640

tattaatgaa tgtgtttgtt acgcgtatta cggatataag gttagtaaaa tcatttctaa     5700

agttgaggaa aagtaaatat aaatggctta aatttcaaca atttgaagtt gaatagatat     5760

gttataatac tattgtagtg tgggatgtta gttactaaag gatgacgctt atatatatga     5820

ctgaatagaa taagcaatag gtttaataat ctattttaaa ttttttgtac tagttttagt     5880

taattagcgg ctgtcagacc aagtttacgg gatatattcc gcttcctcgc tcactgactc     5940

gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt     6000

cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt     6060

ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg     6120

cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc     6180

tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct     6240

cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca     6300

cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     6360

cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt     6420

tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg     6480

ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac     6540

cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat     6600

ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct     6660

cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgttacga acagatcgac     6720

aatgtaacta ctagagcgaa gatcctttga tcttttctac ggggtctgac gctcagtgga     6780

acgaaaactc acgttaaggg atttggtcat ggctagtgct tggaaagcaa gcaaagaaaa     6840

aaggcgacag attactctgt cgcctttttt cctgactcat aaccaagcca attctcgaac     6900

cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat     6960

cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt     7020

cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc     7080

cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat     7140

cgccgtgggt cacgacgaga tcctcgccgt cgggcatacg cgccttgagc ctggcgaaca     7200

gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg     7260

cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg     7320

tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg     7380

caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt     7440

cccttcccgc ttcagtgaca acgtcgagca cagccgcgca aggaacgccc gtcgtggcca     7500

gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct     7560

tgacaaaaag aaccgggcga ccctgcgctg acagccggaa cacggcggca tcagagcagc     7620

cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcc gccggagaac     7680

ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag     7740

atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc     7800

agggcttccc aaccttacca gagggcggcc caactggcaa ttccgacgtc tgtgcaagta     7860

ctactgttct gcagtcactt gcctaagata ctagctccgg caaaaaaacg ggcaaggtgt     7920

caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt     7980

ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg     8040

agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaatttcggc     8100

cggcttgtcg acgacggcgg tctccgtcgt caggatcatc cgggcaagct ttccacacat     8160

tataggtaca aaaagatgcg aaagcaaata aattttttgg gtttgtaccg tacaccactg     8220

agaccgcggt ggttgaccag acaaaccacg aagctgtcac cggatgtgct ttccggtctg     8280

atgagtccgt gaggac                                                     8296


<210>  2
<211>  8293
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha-CLTA-4 Thermal Switching Circuit

<400>  2
gaaacagcct ctacaaataa ttttgtttaa gggcccaagt tcacgagaac gatatagaaa       60

gttccaggag aaaggtacca tgaaatacct gctgccgacc gctgctgctg gtctgctgct      120

cctcgctgcc cagccggcga tggccgcgca ggtgcaactt gttgagtcgg gtggggggct      180

tgcgcagcct ggcggctcct tacgccttag ttgtgccgcg tcagggagta cgatctcttc      240

cgtggccgtt gggtggtatc gtcaaacccc aggcaatcaa cgtgagtggg tggccacttc      300

atctaccagc agtacgacag ctacttacgc ggactcagtg aaggggcgtt tcactattag      360

ccgtgacaac gcgaaaaaca cgatttattt gcagatgaac tcactgaagc ctgaggatac      420

agcggtttac tattgtaaga ctggccttac caactggggg cgtggtacac aagtaacggt      480

gtcatctggc gggttgccag aaactggagg gcatcatcat caccaccact aggtaaggca      540

agctcgtact gggagttcta atgcgtaaag gcgaagagct gttcactggt gtcgtcccta      600

ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg      660

aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc      720

cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt      780

atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc      840

aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat      900

ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg      960

gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg     1020

ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac gtggaggatg     1080

gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc     1140

tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga     1200

aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg     1260

atgaactgta caaatgatga actgggcaca acagagtaat aattcaaaag agatgtcggt     1320

ttaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgccccaa     1380

tgataacccc aagggaagtt ttagtcaaaa gcctccggat gtcactcaat gaagtcgcag     1440

ggttgaggcg atgaggggtc cttccaggca tcaaataaaa cgaaaggctc agtcgaaaga     1500

ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc     1560

gccgccctag acttaggcgt tcggctgcgg cgagcggtat cagctcactc aaagaatcct     1620

tagctttcgc tgaggatgat ttctgctaga ggcagaggtg gttgtgttgc gatcgagtat     1680

caccgcaagg gataaatatc taacaccgtg cgtgttgact attttacctc tggcggtgat     1740

aatggttgca tgtactaagg aggttgtatg gaacaacgca taaccctgaa agattatgca     1800

atgcgctttg ggcaaaccaa gacagctaaa agatctctca cctaccaaac aatgcccccc     1860

tgcaaaaaat aaattcatat aaaaaacata cagataacca tctgcggtga taaattatct     1920

ctggcggtgt tgacataaat accactggcg gtgatactga gcacatcagc aggacgcact     1980

gaccaccatg aaggtgacgc tcatgactta cttgctgaat ctcaggagtt tatgaccttt     2040

tttttttaga tcctatcggt atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg     2100

atgctacgac ttcaccggag cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg     2160

gctgggacgt cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg     2220

accggaagcg cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgtttgacg     2280

tgatcgtggc gtaccgggta gatcggttga cccgatcgat ccggcatctt cagcagctgg     2340

tccactgggc cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata     2400

cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat     2460

tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt caatatccgc gccgggaaat     2520

accgaggatc cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc     2580

tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca     2640

accacgagcc gctgcatctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga     2700

aggactactt cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg     2760

cgctgaagcg atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga     2820

ccgtccgaga cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc     2880

agctggaggc gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta     2940

ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt     3000

tcgccggggg aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc     3060

actgcgggaa cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtac     3120

tggatctgct cggggacgcg gagcgtctgg agaaagtctg ggtagcgggc tcggactccg     3180

cggtcgaact cgcggaggtg aacgcggagc tggtggacct gacgtcgctg atcggctccc     3240

cggcctaccg ggcgggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg     3300

ccgcgcggca agaggagctg gagggcctgg aggctcgccc gtctggctgg gagtggcgcg     3360

agaccgggca gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct     3420

ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga     3480

tcgacttcgg ggatcttcag gagtacgagc agcatctcag gctcggcagc gtggtcgaac     3540

ggctacacac cgggatgtcg ggttcagcag caaacgacga aaactacgct gcacaccgat     3600

gatgcttaag tcgaacagaa agtaatcgta tgagctcgac accatcgaat ggcgcaaaac     3660

ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtata     3720

agaaggagat atacatatga gcacaaaaaa gaatccatta acacaagagc agcttgagga     3780

cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct taacccagga     3840

atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctctat ttaatggcat     3900

caatgcatta aatgctcata acgccgcatt gcttacaaaa attctcaaag ttagcgttga     3960

agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca     4020

gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt     4080

ctcacctgag cctagaacct gtaccaaagg tgatgcggag agatgggtaa gcacaaccaa     4140

aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac     4200

aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt     4260

tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact     4320

gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc     4380

atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac     4440

gtttggctga ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat     4500

ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact     4560

agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac     4620

tatatccgga ttggcttcct gagtgatacc ttaccctgcc tatttgccct ctcccagggt     4680

tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg     4740

ttacgttgga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggcaaaa     4800

gtgaatccag gattatgtgt attgcgttag atttattgat tgcccaagaa aaagcaggat     4860

ttgaggtacc agcactcaaa aagtgacgtc actttttatc ctaaaaacta aaagtgatag     4920

cacttttaat tataagaagt tagaatatta atcatttgct taattgtaca atataatgta     4980

caattgtttt atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat     5040

ttccgccaaa atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt     5100

gtaacaagta aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct     5160

atgcaagaaa cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga     5220

gatgaacaat tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat     5280

gattaaggct tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa     5340

caaaagcaat ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc     5400

tggattagga aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat     5460

tacagatgaa catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc     5520

aaaagatcac tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga     5580

tccttatttt atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat     5640

taatgaatgt gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt     5700

tgaggaaaag taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt     5760

ataatactat tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg     5820

aatagaataa gcaataggtt taataatcta ttttaaattt tttgtactag ttttagttaa     5880

ttagcggctg tcagaccaag tttacgggat atattccgct tcctcgctca ctgactcgct     5940

acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct     6000

ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc     6060

cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga     6120

aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct     6180

cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat     6240

tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga     6300

accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     6360

ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag     6420

tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc     6480

ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc     6540

cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc     6600

aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt     6660

caaatgtagc acctgaagtc agccccatac gatataagtt gttacgaaca gatcgacaat     6720

gtaactacta gagcgaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     6780

aaaactcacg ttaagggatt tggtcatggc tagtgcttgg aaagcaagca aagaaaaaag     6840

gcgacagatt actctgtcgc cttttttcct gactcataac caagccaatt ctcgaacccc     6900

agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg     6960

gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag     7020

caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac     7080

agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc     7140

cgtgggtcac gacgagatcc tcgccgtcgg gcatacgcgc cttgagcctg gcgaacagtt     7200

cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt     7260

ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag     7320

ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag     7380

gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc     7440

ttcccgcttc agtgacaacg tcgagcacag ccgcgcaagg aacgcccgtc gtggccagcc     7500

acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga     7560

caaaaagaac cgggcgaccc tgcgctgaca gccggaacac ggcggcatca gagcagccga     7620

ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagccgcc ggagaacctg     7680

cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc     7740

ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg     7800

gcttcccaac cttaccagag ggcggcccaa ctggcaattc cgacgtctgt gcaagtacta     7860

ctgttctgca gtcacttgcc taagatacta gctccggcaa aaaaacgggc aaggtgtcac     7920

caccctgccc tttttcttta aaaccgaaaa gattacttcg cgtttgccac ctgacgtcta     7980

agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag gcccacccgt gaaggtgagc     8040

cagtgagttg attgctacgt aattagttag ttagccctta gtgactcgaa tttcggccgg     8100

cttgtcgacg acggcggtct ccgtcgtcag gatcatccgg gcaagctttc cacacattat     8160

aggtacaaaa agatgcgaaa gcaaataaat tttttgggtt tgtaccgtac accactgaga     8220

ccgcggtggt tgaccagaca aaccacgaag ctgtcaccgg atgtgctttc cggtctgatg     8280

agtccgtgag gac                                                        8293


<210>  3
<211>  9061
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thermal Switching Circuit Library Screen Plasmid Design


<220>
<221>  misc_feature
<222>  (4455)..(4460)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5999)..(6004)
<223>  n is a, c, g, or t

<400>  3
cagatcgaca atgtaactac tagagcgaag atcctttgat cttttctacg gggtctgacg       60

ctcagtggaa cgaaaactca cgttaaggga tttggtcatg gctagtgctt ggaaagcaag      120

caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata accaagccaa      180

ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc      240

gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc      300

caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac      360

ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca      420

agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc gccttgagcc      480

tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga      540

caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga      600

atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata      660

ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata      720

gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa ggaacgcccg      780

tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca      840

ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac acggcggcat      900

cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagccg      960

ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct     1020

cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt     1080

ttactttgca gggcttccca accttaccag agggcggccc aactggcaat tccgacgtct     1140

gtgcaagtac tactgttctg cagtcacttg cctaagatac tagctccggc aaaaaaacgg     1200

gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc     1260

acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc     1320

gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg     1380

aatttcggcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc gggcaagctt     1440

tccacacatt ataggtacaa aaagatgcga aagcaaataa attttttggg tttgtaccgt     1500

acaccactga gaccgcggtg gttgaccaga caaaccacga agctgtcacc ggatgtgctt     1560

tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaagggcc     1620

caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat     1680

catgctaagg aggttttcta atgcgtaaag gcgaagagct gttcactggt gtcgtcccta     1740

ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg     1800

aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc     1860

cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt     1920

atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc     1980

aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat     2040

ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg     2100

gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg     2160

ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac gtggaggatg     2220

gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc     2280

tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga     2340

aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg     2400

atgaactgta caaatgatga tacgcggtag tttatcacag ttaaattgct aacgcagtca     2460

ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga     2520

tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca     2580

ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt     2640

tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct     2700

cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg     2760

gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg     2820

cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag     2880

cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat     2940

ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg     3000

ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt     3060

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac     3120

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg     3180

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat     3240

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa     3300

gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc     3360

gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat     3420

gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca     3480

aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc     3540

gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct     3600

ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg     3660

aactgggcac aacagagtaa taattcaaaa gagatgtcgg tttaacgcat gagaaagccc     3720

ccggaagatc accttccggg ggctttttta ttgcgcccca atgataaccc caagggaagt     3780

tttagtcaaa agcctccgga tgtcactcaa tgaagtcgca gggttgaggc gatgaggggt     3840

ccttccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc     3900

tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacttaggcg     3960

ttcggctgcg gcgagcggta tcagctcact caaagaatcc ttagctttcg ctgaggatga     4020

tttctgctag aggcagaggt ggttgtgttg cgatcgagta tcaccgcaag ggataaatat     4080

ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag     4140

gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt gggcaaacca     4200

agacagctaa aagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata     4260

taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa     4320

taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg     4380

ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt     4440

gatacgaaac gaagnnnnnn tcggtrtgag agccctggta gtcatccgcc tgtcccgcgt     4500

caccgatgct acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca     4560

gcgcggctgg gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc     4620

gttcgaccgg aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt     4680

tgacgtgatc gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca     4740

gctggtccac tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt     4800

cgatacgacg acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat     4860

ggaattagaa gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg     4920

gaaataccga ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg     4980

gcggctggtg ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt     5040

cgacaaccac gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc     5100

gccgaaggac tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc     5160

taccgcgctg aagcgatcga tgatctccga ggcgatgctc gggtacgcga ctctgaacgg     5220

taagaccgtc cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg     5280

tgagcagctg gaggcgctgc gcgccgagct cgtgaagacc tcccgggcga agcccgcggt     5340

gtctaccccg tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta     5400

caagttcgcc gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc     5460

gaagcactgc gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca     5520

ggtactggat ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga     5580

ctccgcggtc gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg     5640

ctccccggcc taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc     5700

gctggccgcg cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg     5760

gcgcgagacc gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa     5820

cacctggctt cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg     5880

cacgatcgac ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt     5940

cgaacggcta cacaccggga tgtcgggttc agcagcaaac gacgaaaact acgctgcann     6000

nnnntgatgc ttaagtcgaa cagaaagtaa tcgtatgagc tcgacaccat cgaatggcgc     6060

aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat     6120

gtataagaag gagatataca tatgagcaca aaaaagaatc cattaacaca agagcagctt     6180

gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttaacc     6240

caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tctatttaat     6300

ggcatcaatg cattaaatgc tcataacgcc gcattgctta caaaaattct caaagttagc     6360

gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt     6420

atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg     6480

atgttctcac ctgagcctag aacctgtacc aaaggtgatg cggagagatg ggtaagcaca     6540

accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca     6600

ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag     6660

gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag     6720

aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg     6780

atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa     6840

gagacgtttg gctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag     6900

cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa     6960

taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga     7020

ggaactatat ccggattggc tctgtcagac caagtttacg agctcgcttg gactcctgtt     7080

gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg     7140

ccgggcgttt tttattggtg agaatccaag cactagggac agtaagacgg gtaagcctgt     7200

tgatgatacc gctgccttac tgggtgcatt agccagtctg aatgacctgt cacgggataa     7260

tccgaagtgg tcagactgga aaatcagagg gcaggaactg ctgaacagca aaaagtcaga     7320

tagcaccaca tagcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt     7380

gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc     7440

cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca     7500

ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg     7560

ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac     7620

atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct     7680

attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa     7740

acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt     7800

tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggacatgt     7860

aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg     7920

agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac     7980

ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt     8040

gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg     8100

atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg     8160

acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca     8220

aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct     8280

tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa     8340

aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact     8400

cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat     8460

aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta     8520

aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg     8580

attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag     8640

ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac     8700

ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag     8760

gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc     8820

acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt     8880

tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac     8940

tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa     9000

aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga     9060

a                                                                     9061


<210>  4
<211>  9061
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thermal Switching Circuit Library Screen Parent Plasmid

<400>  4
cagatcgaca atgtaactac tagagcgaag atcctttgat cttttctacg gggtctgacg       60

ctcagtggaa cgaaaactca cgttaaggga tttggtcatg gctagtgctt ggaaagcaag      120

caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata accaagccaa      180

ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc      240

gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc      300

caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac      360

ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca      420

agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc gccttgagcc      480

tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga      540

caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga      600

atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata      660

ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata      720

gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa ggaacgcccg      780

tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca      840

ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac acggcggcat      900

cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagccg      960

ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct     1020

cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt     1080

ttactttgca gggcttccca accttaccag agggcggccc aactggcaat tccgacgtct     1140

gtgcaagtac tactgttctg cagtcacttg cctaagatac tagctccggc aaaaaaacgg     1200

gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc     1260

acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc     1320

gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg     1380

aatttcggcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc gggcaagctt     1440

tccacacatt ataggtacaa aaagatgcga aagcaaataa attttttggg tttgtaccgt     1500

acaccactga gaccgcggtg gttgaccaga caaaccacga agctgtcacc ggatgtgctt     1560

tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaagggcc     1620

caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat     1680

catgctaagg aggttttcta atgcgtaaag gcgaagagct gttcactggt gtcgtcccta     1740

ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg     1800

aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc     1860

cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt     1920

atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc     1980

aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat     2040

ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg     2100

gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg     2160

ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac gtggaggatg     2220

gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc     2280

tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga     2340

aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg     2400

atgaactgta caaatgatga tacgcggtag tttatcacag ttaaattgct aacgcagtca     2460

ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga     2520

tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca     2580

ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt     2640

tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct     2700

cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg     2760

gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg     2820

cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag     2880

cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat     2940

ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg     3000

ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt     3060

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac     3120

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg     3180

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat     3240

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa     3300

gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc     3360

gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat     3420

gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca     3480

aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc     3540

gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct     3600

ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg     3660

aactgggcac aacagagtaa taattcaaaa gagatgtcgg tttaacgcat gagaaagccc     3720

ccggaagatc accttccggg ggctttttta ttgcgcccca atgataaccc caagggaagt     3780

tttagtcaaa agcctccgga tgtcactcaa tgaagtcgca gggttgaggc gatgaggggt     3840

ccttccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc     3900

tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacttaggcg     3960

ttcggctgcg gcgagcggta tcagctcact caaagaatcc ttagctttcg ctgaggatga     4020

tttctgctag aggcagaggt ggttgtgttg cgatcgagta tcaccgcaag ggataaatat     4080

ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag     4140

gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt gggcaaacca     4200

agacagctaa aagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata     4260

taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa     4320

taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg     4380

ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt     4440

gatacgaaac gaaggggata tcggtatgag agccctggta gtcatccgcc tgtcccgcgt     4500

caccgatgct acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca     4560

gcgcggctgg gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc     4620

gttcgaccgg aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt     4680

tgacgtgatc gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca     4740

gctggtccac tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt     4800

cgatacgacg acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat     4860

ggaattagaa gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg     4920

gaaataccga ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg     4980

gcggctggtg ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt     5040

cgacaaccac gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc     5100

gccgaaggac tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc     5160

taccgcgctg aagcgatcga tgatctccga ggcgatgctc gggtacgcga ctctgaacgg     5220

taagaccgtc cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg     5280

tgagcagctg gaggcgctgc gcgccgagct cgtgaagacc tcccgggcga agcccgcggt     5340

gtctaccccg tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta     5400

caagttcgcc gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc     5460

gaagcactgc gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca     5520

ggtactggat ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga     5580

ctccgcggtc gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg     5640

ctccccggcc taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc     5700

gctggccgcg cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg     5760

gcgcgagacc gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa     5820

cacctggctt cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg     5880

cacgatcgac ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt     5940

cgaacggcta cacaccggga tgtcgggttc agcagcaaac gacgaaaact acgctgcatc     6000

ggtttgatgc ttaagtcgaa cagaaagtaa tcgtatgagc tcgacaccat cgaatggcgc     6060

aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat     6120

gtataagaag gagatataca tatgagcaca aaaaagaatc cattaacaca agagcagctt     6180

gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttaacc     6240

caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tctatttaat     6300

ggcatcaatg cattaaatgc tcataacgcc gcattgctta caaaaattct caaagttagc     6360

gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt     6420

atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg     6480

atgttctcac ctgagcctag aacctgtacc aaaggtgatg cggagagatg ggtaagcaca     6540

accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca     6600

ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag     6660

gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag     6720

aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg     6780

atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa     6840

gagacgtttg gctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag     6900

cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa     6960

taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga     7020

ggaactatat ccggattggc tctgtcagac caagtttacg agctcgcttg gactcctgtt     7080

gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg     7140

ccgggcgttt tttattggtg agaatccaag cactagggac agtaagacgg gtaagcctgt     7200

tgatgatacc gctgccttac tgggtgcatt agccagtctg aatgacctgt cacgggataa     7260

tccgaagtgg tcagactgga aaatcagagg gcaggaactg ctgaacagca aaaagtcaga     7320

tagcaccaca tagcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt     7380

gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc     7440

cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca     7500

ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg     7560

ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac     7620

atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct     7680

attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa     7740

acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt     7800

tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggacatgt     7860

aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg     7920

agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac     7980

ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt     8040

gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg     8100

atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg     8160

acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca     8220

aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct     8280

tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa     8340

aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact     8400

cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat     8460

aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta     8520

aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg     8580

attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag     8640

ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac     8700

ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag     8760

gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc     8820

acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt     8880

tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac     8940

tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa     9000

aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga     9060

a                                                                     9061


<210>  5
<211>  9061
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thermal Switching Circuit Library Screen Hit #2

<400>  5
cagatcgaca atgtaactac tagagcgaag atcctttgat cttttctacg gggtctgacg       60

ctcagtggaa cgaaaactca cgttaaggga tttggtcatg gctagtgctt ggaaagcaag      120

caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata accaagccaa      180

ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc      240

gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc      300

caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac      360

ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca      420

agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc gccttgagcc      480

tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga      540

caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga      600

atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata      660

ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata      720

gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa ggaacgcccg      780

tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca      840

ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac acggcggcat      900

cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagccg      960

ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct     1020

cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt     1080

ttactttgca gggcttccca accttaccag agggcggccc aactggcaat tccgacgtct     1140

gtgcaagtac tactgttctg cagtcacttg cctaagatac tagctccggc aaaaaaacgg     1200

gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc     1260

acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc     1320

gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg     1380

aatttcggcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc gggcaagctt     1440

tccacacatt ataggtacaa aaagatgcga aagcaaataa attttttggg tttgtaccgt     1500

acaccactga gaccgcggtg gttgaccaga caaaccacga agctgtcacc ggatgtgctt     1560

tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaagggcc     1620

caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat     1680

catgctaagg aggttttcta atgcgtaaag gcgaagagct gttcactggt gtcgtcccta     1740

ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg     1800

aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc     1860

cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt     1920

atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc     1980

aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat     2040

ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg     2100

gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg     2160

ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac gtggaggatg     2220

gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc     2280

tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga     2340

aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg     2400

atgaactgta caaatgatga tacgcggtag tttatcacag ttaaattgct aacgcagtca     2460

ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga     2520

tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca     2580

ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt     2640

tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct     2700

cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg     2760

gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg     2820

cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag     2880

cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat     2940

ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg     3000

ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt     3060

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac     3120

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg     3180

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat     3240

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa     3300

gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc     3360

gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat     3420

gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca     3480

aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc     3540

gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct     3600

ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg     3660

aactgggcac aacagagtaa taattcaaaa gagatgtcgg tttaacgcat gagaaagccc     3720

ccggaagatc accttccggg ggctttttta ttgcgcccca atgataaccc caagggaagt     3780

tttagtcaaa agcctccgga tgtcactcaa tgaagtcgca gggttgaggc gatgaggggt     3840

ccttccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc     3900

tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacttaggcg     3960

ttcggctgcg gcgagcggta tcagctcact caaagaatcc ttagctttcg ctgaggatga     4020

tttctgctag aggcagaggt ggttgtgttg cgatcgagta tcaccgcaag ggataaatat     4080

ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag     4140

gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt gggcaaacca     4200

agacagctaa aagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata     4260

taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa     4320

taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg     4380

ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt     4440

gatacgaaac gaagatccta tcggtatgag agccctggta gtcatccgcc tgtcccgcgt     4500

caccgatgct acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca     4560

gcgcggctgg gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc     4620

gttcgaccgg aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt     4680

tgacgtgatc gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca     4740

gctggtccac tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt     4800

cgatacgacg acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat     4860

ggaattagaa gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg     4920

gaaataccga ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg     4980

gcggctggtg ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt     5040

cgacaaccac gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc     5100

gccgaaggac tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc     5160

taccgcgctg aagcgatcga tgatctccga ggcgatgctc gggtacgcga ctctgaacgg     5220

taagaccgtc cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg     5280

tgagcagctg gaggcgctgc gcgccgagct cgtgaagacc tcccgggcga agcccgcggt     5340

gtctaccccg tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta     5400

caagttcgcc gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc     5460

gaagcactgc gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca     5520

ggtactggat ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga     5580

ctccgcggtc gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg     5640

ctccccggcc taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc     5700

gctggccgcg cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg     5760

gcgcgagacc gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa     5820

cacctggctt cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg     5880

cacgatcgac ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt     5940

cgaacggcta cacaccggga tgtcgggttc agcagcaaac gacgaaaact acgctgcaca     6000

ccgatgatgc ttaagtcgaa cagaaagtaa tcgtatgagc tcgacaccat cgaatggcgc     6060

aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat     6120

gtataagaag gagatataca tatgagcaca aaaaagaatc cattaacaca agagcagctt     6180

gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttaacc     6240

caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tctatttaat     6300

ggcatcaatg cattaaatgc tcataacgcc gcattgctta caaaaattct caaagttagc     6360

gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt     6420

atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg     6480

atgttctcac ctgagcctag aacctgtacc aaaggtgatg cggagagatg ggtaagcaca     6540

accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca     6600

ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag     6660

gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag     6720

aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg     6780

atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa     6840

gagacgtttg gctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag     6900

cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa     6960

taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga     7020

ggaactatat ccggattggc tctgtcagac caagtttacg agctcgcttg gactcctgtt     7080

gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg     7140

ccgggcgttt tttattggtg agaatccaag cactagggac agtaagacgg gtaagcctgt     7200

tgatgatacc gctgccttac tgggtgcatt agccagtctg aatgacctgt cacgggataa     7260

tccgaagtgg tcagactgga aaatcagagg gcaggaactg ctgaacagca aaaagtcaga     7320

tagcaccaca tagcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt     7380

gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc     7440

cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca     7500

ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg     7560

ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac     7620

atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct     7680

attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa     7740

acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt     7800

tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggacatgt     7860

aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg     7920

agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac     7980

ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt     8040

gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg     8100

atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg     8160

acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca     8220

aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct     8280

tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa     8340

aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact     8400

cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat     8460

aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta     8520

aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg     8580

attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag     8640

ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac     8700

ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag     8760

gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc     8820

acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt     8880

tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac     8940

tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa     9000

aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga     9060

a                                                                     9061


<210>  6
<211>  9061
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thermal Switching Circuit Library Screen Hit #7

<400>  6
cagatcgaca atgtaactac tagagcgaag atcctttgat cttttctacg gggtctgacg       60

ctcagtggaa cgaaaactca cgttaaggga tttggtcatg gctagtgctt ggaaagcaag      120

caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata accaagccaa      180

ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc      240

gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc      300

caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac      360

ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca      420

agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc gccttgagcc      480

tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga      540

caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga      600

atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata      660

ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata      720

gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa ggaacgcccg      780

tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca      840

ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac acggcggcat      900

cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagccg      960

ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct     1020

cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt     1080

ttactttgca gggcttccca accttaccag agggcggccc aactggcaat tccgacgtct     1140

gtgcaagtac tactgttctg cagtcacttg cctaagatac tagctccggc aaaaaaacgg     1200

gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc     1260

acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc     1320

gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg     1380

aatttcggcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc gggcaagctt     1440

tccacacatt ataggtacaa aaagatgcga aagcaaataa attttttggg tttgtaccgt     1500

acaccactga gaccgcggtg gttgaccaga caaaccacga agctgtcacc ggatgtgctt     1560

tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaagggcc     1620

caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat     1680

catgctaagg aggttttcta atgcgtaaag gcgaagagct gttcactggt gtcgtcccta     1740

ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg     1800

aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc     1860

cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt     1920

atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc     1980

aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat     2040

ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg     2100

gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg     2160

ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac gtggaggatg     2220

gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc     2280

tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga     2340

aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg     2400

atgaactgta caaatgatga tacgcggtag tttatcacag ttaaattgct aacgcagtca     2460

ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga     2520

tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca     2580

ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt     2640

tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct     2700

cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg     2760

gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg     2820

cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag     2880

cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat     2940

ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg     3000

ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt     3060

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac     3120

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg     3180

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat     3240

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa     3300

gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc     3360

gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat     3420

gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca     3480

aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc     3540

gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct     3600

ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg     3660

aactgggcac aacagagtaa taattcaaaa gagatgtcgg tttaacgcat gagaaagccc     3720

ccggaagatc accttccggg ggctttttta ttgcgcccca atgataaccc caagggaagt     3780

tttagtcaaa agcctccgga tgtcactcaa tgaagtcgca gggttgaggc gatgaggggt     3840

ccttccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc     3900

tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacttaggcg     3960

ttcggctgcg gcgagcggta tcagctcact caaagaatcc ttagctttcg ctgaggatga     4020

tttctgctag aggcagaggt ggttgtgttg cgatcgagta tcaccgcaag ggataaatat     4080

ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag     4140

gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt gggcaaacca     4200

agacagctaa aagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata     4260

taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa     4320

taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg     4380

ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt     4440

gatacgaaac gaagctacaa tcggtatgag agccctggta gtcatccgcc tgtcccgcgt     4500

caccgatgct acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca     4560

gcgcggctgg gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc     4620

gttcgaccgg aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt     4680

tgacgtgatc gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca     4740

gctggtccac tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt     4800

cgatacgacg acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat     4860

ggaattagaa gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg     4920

gaaataccga ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg     4980

gcggctggtg ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt     5040

cgacaaccac gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc     5100

gccgaaggac tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc     5160

taccgcgctg aagcgatcga tgatctccga ggcgatgctc gggtacgcga ctctgaacgg     5220

taagaccgtc cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg     5280

tgagcagctg gaggcgctgc gcgccgagct cgtgaagacc tcccgggcga agcccgcggt     5340

gtctaccccg tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta     5400

caagttcgcc gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc     5460

gaagcactgc gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca     5520

ggtactggat ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga     5580

ctccgcggtc gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg     5640

ctccccggcc taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc     5700

gctggccgcg cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg     5760

gcgcgagacc gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa     5820

cacctggctt cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg     5880

cacgatcgac ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt     5940

cgaacggcta cacaccggga tgtcgggttc agcagcaaac gacgaaaact acgctgcacc     6000

ttattgatgc ttaagtcgaa cagaaagtaa tcgtatgagc tcgacaccat cgaatggcgc     6060

aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat     6120

gtataagaag gagatataca tatgagcaca aaaaagaatc cattaacaca agagcagctt     6180

gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttaacc     6240

caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tctatttaat     6300

ggcatcaatg cattaaatgc tcataacgcc gcattgctta caaaaattct caaagttagc     6360

gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt     6420

atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg     6480

atgttctcac ctgagcctag aacctgtacc aaaggtgatg cggagagatg ggtaagcaca     6540

accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca     6600

ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag     6660

gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag     6720

aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg     6780

atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa     6840

gagacgtttg gctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag     6900

cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa     6960

taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga     7020

ggaactatat ccggattggc tctgtcagac caagtttacg agctcgcttg gactcctgtt     7080

gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg     7140

ccgggcgttt tttattggtg agaatccaag cactagggac agtaagacgg gtaagcctgt     7200

tgatgatacc gctgccttac tgggtgcatt agccagtctg aatgacctgt cacgggataa     7260

tccgaagtgg tcagactgga aaatcagagg gcaggaactg ctgaacagca aaaagtcaga     7320

tagcaccaca tagcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt     7380

gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc     7440

cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca     7500

ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg     7560

ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac     7620

atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct     7680

attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa     7740

acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt     7800

tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggacatgt     7860

aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg     7920

agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac     7980

ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt     8040

gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg     8100

atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg     8160

acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca     8220

aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct     8280

tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa     8340

aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact     8400

cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat     8460

aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta     8520

aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg     8580

attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag     8640

ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac     8700

ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag     8760

gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc     8820

acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt     8880

tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac     8940

tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa     9000

aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga     9060

a                                                                     9061


<210>  7
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBS

<400>  7
atcctatcgg tatg                                                         14


<210>  8
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBS

<400>  8
ctacaatcgg tatg                                                         14


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ssrA degradation tag

<400>  9

Gly Ser Ala Ala Asn Asp Glu Asn Tyr Ala Ala His Arg 
1               5                   10              


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ssrA degradation tag

<400>  10

Gly Ser Ala Ala Asn Asp Glu Asn Tyr Ala Ala Pro Tyr 
1               5                   10              


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  temperature-sensitive terminator

<400>  11
atgacttact tgctgaatct caggagttta tgaccttttt ttttt                       45


