                         SEQUENCE LISTING

<110>  WUXI BIOLOGICS (SHANGHAI) CO., LTD.
       WUXI BIOLOGICS IRELAND LIMITED
 
<120>  A Bispecific anti-PD-L1/VEGF Antibody and Uses thereof

<130>  IEC216015PCT

<150>  PCT/CN2021/084447
<151>  2021-03-31

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR1

<400>  1

Gly Phe Thr Phe Ser Ser Tyr Ala Met Ser 
1               5                   10  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR2

<400>  2

Gly Ile Ser Gly Ser Gly Gly Phe Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR3

<400>  3

Pro Pro Arg Gly Tyr Asn Tyr Gly Pro Phe Asp Tyr 
1               5                   10          


<210>  4
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR1

<400>  4

Gly Gly Asn Asn Ile Gly Ser Lys Ser Val His 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR2

<400>  5

Asp Asp Ser Asp Arg Pro Ser 
1               5           


<210>  6
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR3

<400>  6

Gln Val Trp Asp Ser Ser Ser Asp His Val Val 
1               5                   10      


<210>  7
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR1

<400>  7

Gly Tyr Thr Phe Thr Asn Tyr Gly Met Asn 
1               5                   10  


<210>  8
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR2

<400>  8

Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe Lys 
1               5                   10                  15      


Arg 
    


<210>  9
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HCDR3

<400>  9

Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 
1               5                   10                  


<210>  10
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR1

<400>  10

Ser Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
1               5                   10      


<210>  11
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR2

<400>  11

Tyr Phe Thr Ser Ser Leu His Ser 
1               5               


<210>  12
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCDR3

<400>  12

Gln Gln Tyr Ser Thr Val Pro Trp Thr 
1               5                   


<210>  13
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH PDL1

<400>  13

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Ser Gly Gly Phe Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Pro Pro Arg Gly Tyr Asn Tyr Gly Pro Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  14
<211>  108
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VL PDL1

<400>  14

Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Val Tyr 
        35                  40                  45              


Asp Asp Ser Asp Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105             


<210>  15
<211>  123
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH VEGF

<400>  15

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 
    50                  55                  60                  


Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


<210>  16
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VL VEGF

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  17
<211>  711
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Heavy chain

<400>  17

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Ser Gly Gly Phe Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Pro Pro Arg Gly Tyr Asn Tyr Gly Pro Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Tyr Val 
    130                 135                 140                 


Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Gln Thr Ala Arg 
145                 150                 155                 160 


Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val His Trp Tyr 
                165                 170                 175     


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Val Tyr Asp Asp Ser 
            180                 185                 190         


Asp Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser Gly 
        195                 200                 205             


Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly Asp Glu Ala 
    210                 215                 220                 


Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His Val Val Phe 
225                 230                 235                 240 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            260                 265                 270         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe 
        275                 280                 285             


Thr Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    290                 295                 300                 


Glu Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala 
305                 310                 315                 320 


Ala Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser 
                325                 330                 335     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            340                 345                 350         


Tyr Tyr Cys Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr 
        355                 360                 365             


Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    370                 375                 380                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
385                 390                 395                 400 


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                405                 410                 415     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            420                 425                 430         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        435                 440                 445             


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
    450                 455                 460                 


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val 
465                 470                 475                 480 


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                485                 490                 495     


Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            500                 505                 510         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        515                 520                 525             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    530                 535                 540                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
545                 550                 555                 560 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                565                 570                 575     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            580                 585                 590         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        595                 600                 605             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    610                 615                 620                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
625                 630                 635                 640 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                645                 650                 655     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            660                 665                 670         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        675                 680                 685             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    690                 695                 700                 


Ser Leu Ser Leu Ser Pro Gly 
705                 710     


<210>  18
<211>  214
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Light chain

<400>  18

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  19
<211>  2133
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of Heavy chain

<400>  19
gaggtgcaac tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagttgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcaggt attagtggta gtggtggttt cacttactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gaaacctcct      300

cgtggataca actatggccc ttttgactac tggggccagg gaaccctggt caccgtctcc      360

tcaggcggcg gaggaagcgg aggaggtggc agcggtggcg gtggctccgg cggaggtggc      420

tcctcctatg tgctgactca gccaccctcg gtgtcagtgg ccccaggaca gacggccagg      480

attacctgtg ggggaaacaa cattggaagt aaaagtgtac actggtacca gcagaagcca      540

ggccaggccc ctgtgctggt cgtctatgat gatagcgacc ggccctcagg gatccctgag      600

cgattctctg gctccaactc tgggaacacg gccaccctga ccatcagcag ggtcgaagcc      660

ggggatgagg ccgactatta ctgtcaggtg tgggatagta gtagtgatca cgtggtattc      720

ggcggaggga ccaagctgac cgtcctaggt ggaggtggca gtggaggcgg aggatctgaa      780

gttcagctgg tggagtctgg cggtggcctg gtgcagccag ggggctcact ccgtttgtcc      840

tgtgcagctt ctggctatac cttcaccaac tatggtatga actgggtccg tcaggccccg      900

ggtaagggcc tggaatgggt tggatggatt aacacctata ccggtgaacc gacctatgct      960

gcggatttca aacgtcgttt cactttcagc ttagacacct ccaagtcgac agcatacctg     1020

cagatgaaca gcctgcgtgc tgaggacact gccgtctatt actgtgcaaa gtacccccac     1080

tattatggga gcagccactg gtatttcgac gtctggggtc aaggaaccct ggtcaccgtc     1140

tcctcggcgt cgaccaaggg cccatccgtc ttccccctgg caccctcctc caagagcacc     1200

tctgggggca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg     1260

gtgtcctgga actcaggcgc tctgaccagc ggcgtgcaca ccttcccggc tgtcctacag     1320

tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag cttgggcacc     1380

cagacctaca tctgcaacgt gaatcacaag cccagcaaca ccaaggtgga caagagagtt     1440

gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaagccgcc     1500

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     1560

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     1620

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     1680

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     1740

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     1800

atctccaaag ccaaagggca gccccgagaa ccacaggtct acaccctgcc cccatcccgg     1860

gaggagatga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     1920

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     1980

cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc     2040

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     2100

tacacgcaga agagcttaag cctgtctccg ggt                                  2133


<210>  20
<211>  642
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of Light chain

<400>  20
gatatccaga tgacccagtc cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc       60

atcacctgca gcgcaagtca ggatattagc aactatttaa actggtatca acagaaacca      120

ggaaaagctc cgaaagtgct gatttacttc acctcctctc tccactctgg agtcccttct      180

cgcttctctg gatccggttc tgggacggat tttactctga ccatcagcag tctgcagcca      240

gaagacttcg caacttatta ctgtcaacag tatagcaccg tgccgtggac gtttggacag      300

ggtaccaagg tggagatcaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      480

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gt                         642


