                         SEQUENCE LISTING

<110>  Adverum Biotechnologies, Inc.
 
<120>  INTRAVITREAL DOSING FOR DELIVERY OF POLYNUCLEOTIDES TO RETINAL 
       CONES

<130>  AVBI-016/02WO 307702-2226

<150>  US 17/705,237
<151>  2022-03-25

<150>  US 63/306,004
<151>  2022-02-02

<150>  US 63/166,600
<151>  2021-03-26

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  745
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Leu Ala Leu Gly Glu 
            580                 585                 590         


Thr Thr Arg Pro Ala Arg Gln Ala Ala Thr Ala Asp Val Asn Thr Gln 
        595                 600                 605             


Gly Val Leu Pro Gly Met Val Trp Gln Asp Arg Asp Val Tyr Leu Gln 
    610                 615                 620                 


Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly His Phe His Pro 
625                 630                 635                 640 


Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile 
                645                 650                 655     


Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Ser Thr Thr Phe Ser 
            660                 665                 670         


Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val 
        675                 680                 685             


Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp 
    690                 695                 700                 


Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn Lys Ser Val Asn Val 
705                 710                 715                 720 


Asp Phe Thr Val Asp Thr Asn Gly Val Tyr Ser Glu Pro Arg Pro Ile 
                725                 730                 735     


Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
            740                 745 


<210>  2
<211>  725
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Phe Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Thr Gly Lys Arg 
    130                 135                 140                 


Ile Asp Asp His Phe Pro Lys Arg Lys Lys Ala Arg Thr Glu Glu Asp 
145                 150                 155                 160 


Ser Lys Pro Ser Thr Ser Ser Asp Ala Glu Ala Gly Pro Ser Gly Ser 
                165                 170                 175     


Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala Ser Ser Leu Gly Ala Asp 
            180                 185                 190         


Thr Met Ser Ala Gly Gly Gly Gly Pro Leu Gly Asp Asn Asn Gln Gly 
        195                 200                 205             


Ala Asp Gly Val Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr 
    210                 215                 220                 


Trp Met Gly Asp Arg Val Val Thr Lys Ser Thr Arg Thr Trp Val Leu 
225                 230                 235                 240 


Pro Ser Tyr Asn Asn His Gln Tyr Arg Glu Ile Lys Ser Gly Ser Val 
                245                 250                 255     


Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly 
            260                 265                 270         


Tyr Phe Asp Phe Asn Arg Phe His Ser His Trp Ser Pro Arg Asp Trp 
        275                 280                 285             


Gln Arg Leu Ile Asn Asn Tyr Trp Gly Phe Arg Pro Arg Ser Leu Arg 
    290                 295                 300                 


Val Lys Ile Phe Asn Ile Gln Val Lys Glu Val Thr Val Gln Asp Ser 
305                 310                 315                 320 


Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Val Gln Val Phe Thr 
                325                 330                 335     


Asp Asp Asp Tyr Gln Leu Pro Tyr Val Val Gly Asn Gly Thr Glu Gly 
            340                 345                 350         


Cys Leu Pro Ala Phe Pro Pro Gln Val Phe Thr Leu Pro Gln Tyr Gly 
        355                 360                 365             


Tyr Ala Thr Leu Asn Arg Asp Asn Thr Glu Asn Pro Thr Glu Arg Ser 
    370                 375                 380                 


Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser Lys Met Leu Arg Thr Gly 
385                 390                 395                 400 


Asn Asn Phe Glu Phe Thr Tyr Asn Phe Glu Glu Val Pro Phe His Ser 
                405                 410                 415     


Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys Leu Ala Asn Pro Leu Val 
            420                 425                 430         


Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr Asn Asn Thr Gly Gly Val 
        435                 440                 445             


Gln Phe Asn Lys Asn Leu Ala Gly Arg Tyr Ala Asn Thr Tyr Lys Asn 
    450                 455                 460                 


Trp Phe Pro Gly Pro Met Gly Arg Thr Gln Gly Trp Asn Leu Gly Ser 
465                 470                 475                 480 


Gly Val Asn Arg Ala Ser Val Ser Ala Phe Ala Thr Thr Asn Arg Met 
                485                 490                 495     


Glu Leu Glu Gly Ala Ser Tyr Gln Val Pro Pro Gln Pro Asn Gly Met 
            500                 505                 510         


Thr Asn Asn Leu Gln Gly Ser Asn Thr Tyr Ala Leu Glu Asn Thr Met 
        515                 520                 525             


Ile Phe Asn Ser Gln Pro Ala Asn Pro Gly Thr Thr Ala Thr Tyr Leu 
    530                 535                 540                 


Glu Gly Asn Met Leu Ile Thr Ser Glu Ser Glu Thr Gln Pro Val Asn 
545                 550                 555                 560 


Arg Val Ala Tyr Asn Val Gly Gly Gln Met Leu Ala His Lys Phe Lys 
                565                 570                 575     


Ser Gly Asp Ala Pro Thr Thr Gly Thr Tyr Asn Leu Gln Glu Ile Val 
            580                 585                 590         


Pro Gly Ser Val Trp Met Glu Arg Asp Val Tyr Leu Gln Gly Pro Ile 
        595                 600                 605             


Trp Ala Lys Ile Pro Glu Thr Gly Ala His Phe His Pro Ser Pro Ala 
    610                 615                 620                 


Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Met Met Leu Ile Lys 
625                 630                 635                 640 


Asn Thr Pro Val Pro Gly Asn Ile Thr Ser Phe Ser Asp Val Pro Val 
                645                 650                 655     


Ser Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Thr Val Glu Met 
            660                 665                 670         


Glu Trp Glu Leu Lys Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile 
        675                 680                 685             


Gln Tyr Thr Asn Asn Tyr Asn Asp Pro Gln Phe Val Asp Phe Ala Pro 
    690                 695                 700                 


Asp Ser Thr Gly Glu Tyr Arg Thr Thr Arg Pro Ile Gly Thr Arg Tyr 
705                 710                 715                 720 


Leu Thr Arg Pro Leu 
                725 


<210>  3
<211>  3918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3
gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg       60

tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag      120

gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag      180

gatcttcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt      240

ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca      300

tgtccaatat gaccgccatg ttggcattga ttattgacta gtcctacagc agccagggtg      360

agattatgag gctgagctga gaatatcaag actgtaccga gtagggggcc ttggcaagtg      420

tggagagccc ggcagctggg gcagagggcg gagtacggtg tgcgtttacg gacctcttca      480

aacgaggtag gaaggtcaga agtcaaaaag ggaacaaatg atgtttaacc acacaaaaat      540

gaaaatccaa tggttggata tccattccaa atacacaaag gcaacggata agtgatccgg      600

gccaggcaca gaaggccatg cacccgtagg attgcactca gagctcccaa atgcatagga      660

atagaagggt gggtgcagga ggctgagggg tggggaaagg gcatgggtgt ttcatgagga      720

cagagcttcc gtttcatgca atgaaaagag tttggagacg gatggtggtg actggactat      780

acacttacac acggtagcga tggtacactt tgtattatgt atattttacc acgatctttt      840

taaagtgtca aaggcaaatg gccaaatggt tccttgtcct atagctgtag cagccatcgg      900

ctgttagtga caaagcccct gagtcaagat gacagcagcc cccataactc ctaatcggct      960

ctcccgcgtg gagtcattta ggagtagtcg cattagagac aagtccaaca tctaatcttc     1020

caccctggcc agggccccag ctggcagcga gggtgggaga ctccgggcag agcagagggc     1080

gctgacattg gggcccggcc tggcttgggt ccctctggcc tttccccagg ggccctcttt     1140

ccttggggct ttcttgggcc gccactgctc ccgctcctct ccccccatcc caccccctca     1200

ccccctcgtt cttcatatcc ttctctagtg ctccctccac tttcatccac ccttctgcaa     1260

gagtgtggga ccacaaatga gttttcacct ggcctgggga cacacgtgcc cccacaggtg     1320

ctgagtgact ttctaggaca gtaatctgct ttaggctaaa atgggacttg atcttctgtt     1380

agccctaatc atcaattagc agagccggtg aaggtgcaga acctaccgcc tttccaggcc     1440

tcctcccacc tctgccacct ccactctcct tcctgggatg tgggggctgg cacacgtgtg     1500

gcccagggca ttggtgggat tgcactgagc tgggtcatta gcgtaatcct ggacaagggc     1560

agacagggcg agcggagggc cagctccggg gctcaggcaa ggctgggggc ttcccccaga     1620

caccccactc ctcctctgct ggacccccac ttcatagggc acttcgtgtt ctcaaagggc     1680

ttccaaatag catggtggcc ttggatgccc agggaagcct cagagttgct tatctccctc     1740

tagacagaag gggaatctcg gtcaagaggg agaggtcgcc ctgttcaagg ccacccagcc     1800

agctcatggc ggtaatggga caaggctggc cagccatccc accctcagaa gggacccggt     1860

ggggcaggtg atctcagagg aggctcactt ctgggtctca cattcttcca gcaaatccct     1920

ctgagccgcc cccgggggct cgcctcagga gcaaggaagc aaggggtggg aggaggaggt     1980

ctaagtccca ggcccaatta agagatcaga tggtgtagga tttgggagct tttaaggtga     2040

agaggcccgg gctgatccca ctggccggta taaagcaccg tgaccctcag gtgacgcacc     2100

agggccggct gccgtcgggg acagggcttt ccatagccca ggtaagtatc aaggttacaa     2160

gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt     2220

tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggcccag     2280

agaggagaca ggccgccacc atggcccagc agtggagcct ccaaaggctc gcaggccgcc     2340

atccgcagga cagctatgag gacagcaccc agtccagcat cttcacctac accaacagca     2400

actccaccag aggccccttc gaaggcccga attaccacat cgctcccaga tgggtgtacc     2460

acctcaccag tgtctggatg atctttgtgg tcactgcatc cgtcttcaca aatgggcttg     2520

tgctggcggc caccatgaag ttcaagaagc tgcgccaccc gctgaactgg atcctggtga     2580

acctggcggt cgctgaccta gcagagaccg tcatcgccag cactatcagc attgtgaacc     2640

aggtctctgg ctacttcgtg ctgggccacc ctatgtgtgt cctggagggc tacaccgtct     2700

ccctgtgtgg gatcacaggt ctctggtctc tggccatcat ttcctgggag aggtggctgg     2760

tggtgtgcaa gccctttggc aatgtgagat ttgatgccaa gctggccatc gtgggcattg     2820

ccttctcctg gatctggtct gctgtgtgga cagccccgcc catctttggt tggagcaggt     2880

actggcccca cggcctgaag acttcatgcg gcccagacgt gttcagcggc agctcgtacc     2940

ccggggtgca gtcttacatg attgtcctca tggtcacctg ctgcatcatc ccactcgcta     3000

tcatcatgct ctgctacctc caagtgtggc tggccatccg agcggtggca aagcagcaga     3060

aagagtctga atccacccag aaggcagaga aggaagtgac gcgcatggtg gtggtgatga     3120

tctttgcgta ctgcgtctgc tggggaccct acaccttctt cgcatgcttt gctgctgcca     3180

accctggtta cgccttccac cctttgatgg ctgccctgcc ggcctacttt gccaaaagtg     3240

ccactatcta caaccccgtt atctatgtct ttatgaaccg gcagtttcga aactgcatct     3300

tgcagctttt cgggaagaag gttgacgatg gctctgaact ctccagcgcc tccaaaacgg     3360

aggtctcatc tgtgtcctcg gtatcgcctg catgaggtct gcctcctacc catcccgccc     3420

accggggctt tggccacctc tcctttcccc ctccttctcc atccctgtaa aataaatgta     3480

atttatcttt gccaaaacca acagacatga taagatacat tgatgagttt ggacaaacca     3540

caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat     3600

ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt     3660

ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg     3720

gtaaaatcga taaggatcct agagcatggc tacgtagata agtagcatgg cgggttaatc     3780

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3840

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3900

gtgagcgagc gagcgcgc                                                   3918


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
ctctcctttc cccctccttc                                                   20


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gcattctagt tgtggtttgt cca                                               23


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe sequence

<400>  6
tgccaaaacc aacagacatg a                                                 21


