                         SEQUENCE LISTING

<110>  BEAM THERAPEUTICS INC.
 
<120>  ADENOSINE DEAMINASE VARIANTS AND USES THEREOF

<130>  180802.048002/PCT

<150>  63/229,057
<151>  2021-08-03

<150>  63/166,778
<151>  2021-03-26

<160>  411   

<170>  PatentIn version 3.5

<210>  1
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  1

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Ser Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp 
                165         


<210>  2
<211>  167
<212>  PRT
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(167)
<223>  TadA*7.10 (ecTadA) polypeptide sequence

<400>  2

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp 
                165         


<210>  3
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at P153 polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (7)..(57)
<223>  Sequence encoding N-terminal nuclear localization signal

<220>
<221>  misc_feature
<222>  (58)..(513)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (514)..(531)
<223>  Sequence encoding a linker

<220>
<221>  misc_feature
<222>  (532)..(1029)
<223>  Sequence encoding TadA*8

<220>
<221>  misc_feature
<222>  (1030)..(1089)
<223>  Sequence encoding Xten20 linker

<220>
<221>  misc_feature
<222>  (1090)..(3954)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3955)..(4002)
<223>  Sequence encoding C-terminal NLS

<220>
<221>  misc_feature
<222>  (4003)..(4008)
<223>  Seqquence encoding GS linker

<220>
<221>  misc_feature
<222>  (4009)..(4089)
<223>  Sequence encoding 3x hemagglutinin (HA) tag

<400>  3
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgcc       60

accagatcct tcatcctgaa gatcgagccc aacgaggaag tgaagaaagg cctctggaaa      120

acccacgagg tgctgaacca cggaatcgcc tactacatga atatcctgaa gctgatccgg      180

caagaggcca tctacgagca ccacgagcag gaccccaaga atcccaagaa ggtgtccaag      240

gccgagatcc aggccgagct gtgggatttc gtgctgaaga tgcagaagtg caacagcttc      300

acacacgagg tggacaagga cgaggtgttc aacatcctga gagagctgta cgaggaactg      360

gtgcccagca gcgtggaaaa gaagggcgaa gccaaccagc tgagcaacaa gtttctgtac      420

cctctggtgg accccaacag ccagtctgga aagggaacag ccagcagcgg cagaaagccc      480

agatggtaca acctgaagat tgccggcgat cccggaggct ctggaggaag ctccgaagtc      540

gagttttccc atgagtactg gatgagacac gcattgactc tcgcaaagag ggctcgagat      600

gaacgcgagg tgcccgtggg ggcagtactc gtgctcaaca atcgcgtaat cggcgaaggt      660

tggaataggg caatcggact ccacgacccc actgcacatg cggaaatcat ggcccttcga      720

cagggagggc ttgtgatgca gaattatcga ctttatgatg cgacgctgta cgtcacgttt      780

gaaccttgcg taatgtgcgc gggagctatg attcactccc gcattggacg agttgtattc      840

ggtgttcgca acgccaagac gggtgccgca ggttcactga tggacgtgct gcatcatcca      900

ggcatgaacc accgggtaga aatcacagaa ggcatattgg cggacgaatg tgcggcgctg      960

ttgtgtcgtt tttttcgcat gcccaggcgg gtctttaacg cccagaaaaa agcacaatcc     1020

tctactgacg gctcttctgg atctgaaaca cctggcacaa gcgagagcgc cacccctgag     1080

agctctggct cctgggaaga agagaagaag aagtgggaag aagataagaa aaaggacccg     1140

ctggccaaga tcctgggcaa gctggctgag tacggactga tccctctgtt catcccctac     1200

accgacagca acgagcccat cgtgaaagaa atcaagtgga tggaaaagtc ccggaaccag     1260

agcgtgcggc ggctggataa ggacatgttc attcaggccc tggaacggtt cctgagctgg     1320

gagagctgga acctgaaagt gaaagaggaa tacgagaagg tcgagaaaga gtacaagacc     1380

ctggaagaga ggatcaaaga ggacatccag gctctgaagg ctctggaaca gtatgagaaa     1440

gagcggcaag aacagctgct gcgggacacc ctgaacacca acgagtaccg gctgagcaag     1500

agaggcctta gaggctggcg ggaaatcatc cagaaatggc tgaaaatgga cgagaacgag     1560

ccctccgaga agtacctgga agtgttcaag gactaccagc ggaagcaccc tagagaggcc     1620

ggcgattaca gcgtgtacga gttcctgtcc aagaaagaga accacttcat ctggcggaat     1680

caccctgagt acccctacct gtacgccacc ttctgcgaga tcgacaagaa aaagaaggac     1740

gccaagcagc aggccacctt cacactggcc gatcctatca atcaccctct gtgggtccga     1800

ttcgaggaaa gaagcggcag caacctgaac aagtacagaa tcctgaccga gcagctgcac     1860

accgagaagc tgaagaaaaa gctgacagtg cagctggacc ggctgatcta ccctacagaa     1920

tctggcggct gggaagagaa gggcaaagtg gacattgtgc tgctgcccag ccggcagttc     1980

tacaaccaga tcttcctgga catcgaggaa aagggcaagc acgccttcac ctacaaggat     2040

gagagcatca agttccctct gaagggcaca ctcggcggag ccagagtgca gttcgacaga     2100

gatcacctga gaagataccc tcacaaggtg gaaagcggca acgtgggcag aatctacttc     2160

aacatgaccg tgaacatcga gcctacagag tccccagtgt ccaagtctct gaagatccac     2220

cgggacgact tccccaaggt ggtcaacttc aagcccaaag aactgaccga gtggatcaag     2280

gacagcaagg gcaagaaact gaagtccggc atcgagtccc tggaaatcgg cctgagagtg     2340

atgagcatcg acctgggaca gagacaggcc gctgccgcct ctattttcga ggtggtggat     2400

cagaagcccg acatcgaagg caagctgttt ttcccaatca agggcaccga gctgtatgcc     2460

gtgcacagag ccagcttcaa catcaagctg cccggcgaga cactggtcaa gagcagagaa     2520

gtgctgcgga aggccagaga ggacaatctg aaactgatga accagaagct caacttcctg     2580

cggaacgtgc tgcacttcca gcagttcgag gacatcaccg agagagagaa gcgggtcacc     2640

aagtggatca gcagacaaga gaacagcgac gtgcccctgg tgtaccagga tgagctgatc     2700

cagatccgcg agctgatgta caagccttac aaggactggg tcgccttcct gaagcagctc     2760

cacaagagac tggaagtcga gatcggcaaa gaagtgaagc actggcggaa gtccctgagc     2820

gacggaagaa agggcctgta cggcatctcc ctgaagaaca tcgacgagat cgatcggacc     2880

cggaagttcc tgctgagatg gtccctgagg cctaccgaac ctggcgaagt gcgtagactg     2940

gaacccggcc agagattcgc catcgaccag ctgaatcacc tgaacgccct gaaagaagat     3000

cggctgaaga agatggccaa caccatcatc atgcacgccc tgggctactg ctacgacgtg     3060

cggaagaaga aatggcaggc taagaacccc gcctgccaga tcatcctgtt cgaggatctg     3120

agcaactaca acccctacga ggaaaggtcc cgcttcgaga acagcaagct catgaagtgg     3180

tccagacgcg agatccccag acaggttgca ctgcagggcg agatctatgg cctgcaagtg     3240

ggagaagtgg gcgctcagtt cagcagcaga ttccacgcca agacaggcag ccctggcatc     3300

agatgtagcg tcgtgaccaa agagaagctg caggacaatc ggttcttcaa gaatctgcag     3360

agagagggca gactgaccct ggacaaaatc gccgtgctga aagagggcga tctgtaccca     3420

gacaaaggcg gcgagaagtt catcagcctg agcaaggatc ggaagtgcgt gaccacacac     3480

gccgacatca acgccgctca gaacctgcag aagcggttct ggacaagaac ccacggcttc     3540

tacaaggtgt actgcaaggc ctaccaggtg gacggccaga ccgtgtacat ccctgagagc     3600

aaggaccaga agcagaagat catcgaagag ttcggcgagg gctacttcat tctgaaggac     3660

ggggtgtacg aatgggtcaa cgccggcaag ctgaaaatca agaagggcag ctccaagcag     3720

agcagcagcg agctggtgga tagcgacatc ctgaaagaca gcttcgacct ggcctccgag     3780

ctgaaaggcg aaaagctgat gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac     3840

aaatggatgg ccgctggcgt gttcttcgga aagctggaac gcatcctgat cagcaagctg     3900

accaaccagt actccatcag caccatcgag gacgacagca gcaagcagtc tatgaaaagg     3960

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat     4020

gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc     4080

gactatgcct aa                                                         4092


<210>  4
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at P153 polypeptide sequence

<400>  4

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Gly Gly Ser Gly Gly Ser Ser 
                165                 170                 175     


Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu 
            180                 185                 190         


Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu 
        195                 200                 205             


Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly 
    210                 215                 220                 


Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly 
225                 230                 235                 240 


Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu Tyr Val 
                245                 250                 255     


Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg 
            260                 265                 270         


Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala 
        275                 280                 285             


Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His Arg Val 
    290                 295                 300                 


Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys 
305                 310                 315                 320 


Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys Lys Ala 
                325                 330                 335     


Gln Ser Ser Thr Asp Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser 
            340                 345                 350         


Glu Ser Ala Thr Pro Glu Ser Ser Gly Ser Trp Glu Glu Glu Lys Lys 
        355                 360                 365             


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
    370                 375                 380                 


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
385                 390                 395                 400 


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
                405                 410                 415     


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
            420                 425                 430         


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
        435                 440                 445             


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
    450                 455                 460                 


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
465                 470                 475                 480 


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
                485                 490                 495     


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
            500                 505                 510         


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
        515                 520                 525             


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
    530                 535                 540                 


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
545                 550                 555                 560 


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
                565                 570                 575     


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
            580                 585                 590         


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
        595                 600                 605             


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
    610                 615                 620                 


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
625                 630                 635                 640 


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
                645                 650                 655     


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
            660                 665                 670         


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
        675                 680                 685             


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
    690                 695                 700                 


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
705                 710                 715                 720 


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
                725                 730                 735     


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
            740                 745                 750         


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
        755                 760                 765             


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
    770                 775                 780                 


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
785                 790                 795                 800 


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
                805                 810                 815     


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
            820                 825                 830         


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
        835                 840                 845             


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
    850                 855                 860                 


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
865                 870                 875                 880 


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
                885                 890                 895     


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
            900                 905                 910         


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
        915                 920                 925             


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
    930                 935                 940                 


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
945                 950                 955                 960 


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
                965                 970                 975     


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
            980                 985                 990         


Asn Ala Leu Lys Glu Asp Arg Leu  Lys Lys Met Ala Asn  Thr Ile Ile 
        995                 1000                 1005             


Met His  Ala Leu Gly Tyr Cys  Tyr Asp Val Arg Lys  Lys Lys Trp 
    1010                 1015                 1020             


Gln Ala  Lys Asn Pro Ala Cys  Gln Ile Ile Leu Phe  Glu Asp Leu 
    1025                 1030                 1035             


Ser Asn  Tyr Asn Pro Tyr Glu  Glu Arg Ser Arg Phe  Glu Asn Ser 
    1040                 1045                 1050             


Lys Leu  Met Lys Trp Ser Arg  Arg Glu Ile Pro Arg  Gln Val Ala 
    1055                 1060                 1065             


Leu Gln  Gly Glu Ile Tyr Gly  Leu Gln Val Gly Glu  Val Gly Ala 
    1070                 1075                 1080             


Gln Phe  Ser Ser Arg Phe His  Ala Lys Thr Gly Ser  Pro Gly Ile 
    1085                 1090                 1095             


Arg Cys  Ser Val Val Thr Lys  Glu Lys Leu Gln Asp  Asn Arg Phe 
    1100                 1105                 1110             


Phe Lys  Asn Leu Gln Arg Glu  Gly Arg Leu Thr Leu  Asp Lys Ile 
    1115                 1120                 1125             


Ala Val  Leu Lys Glu Gly Asp  Leu Tyr Pro Asp Lys  Gly Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ser Leu Ser Lys  Asp Arg Lys Cys Val  Thr Thr His 
    1145                 1150                 1155             


Ala Asp  Ile Asn Ala Ala Gln  Asn Leu Gln Lys Arg  Phe Trp Thr 
    1160                 1165                 1170             


Arg Thr  His Gly Phe Tyr Lys  Val Tyr Cys Lys Ala  Tyr Gln Val 
    1175                 1180                 1185             


Asp Gly  Gln Thr Val Tyr Ile  Pro Glu Ser Lys Asp  Gln Lys Gln 
    1190                 1195                 1200             


Lys Ile  Ile Glu Glu Phe Gly  Glu Gly Tyr Phe Ile  Leu Lys Asp 
    1205                 1210                 1215             


Gly Val  Tyr Glu Trp Val Asn  Ala Gly Lys Leu Lys  Ile Lys Lys 
    1220                 1225                 1230             


Gly Ser  Ser Lys Gln Ser Ser  Ser Glu Leu Val Asp  Ser Asp Ile 
    1235                 1240                 1245             


Leu Lys  Asp Ser Phe Asp Leu  Ala Ser Glu Leu Lys  Gly Glu Lys 
    1250                 1255                 1260             


Leu Met  Leu Tyr Arg Asp Pro  Ser Gly Asn Val Phe  Pro Ser Asp 
    1265                 1270                 1275             


Lys Trp  Met Ala Ala Gly Val  Phe Phe Gly Lys Leu  Glu Arg Ile 
    1280                 1285                 1290             


Leu Ile  Ser Lys Leu Thr Asn  Gln Tyr Ser Ile Ser  Thr Ile Glu 
    1295                 1300                 1305             


Asp Asp  Ser Ser Lys Gln Ser  Met Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Gly Ser Tyr  Pro Tyr Asp 
    1325                 1330                 1335             


Val Pro  Asp Tyr Ala Tyr Pro  Tyr Asp Val Pro Asp  Tyr Ala Tyr 
    1340                 1345                 1350             


Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1355                 1360     


<210>  5
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at K255 polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (7)..(57)
<223>  Sequence encoding N-terminal nuclear localization signal (NLS)

<220>
<221>  misc_feature
<222>  (58)..(822)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (823)..(840)
<223>  Sequence encoding a linker

<220>
<221>  misc_feature
<222>  (841)..(1338)
<223>  Sequence encoding TadA*8

<220>
<221>  misc_feature
<222>  (1339)..(1398)
<223>  Sequence encoding Xten20 linker

<220>
<221>  misc_feature
<222>  (1399)..(3954)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3955)..(4002)
<223>  Sequence encoding C-terminal NLS

<220>
<221>  misc_feature
<222>  (4003)..(4008)
<223>  Sequence encoding a GS linker

<220>
<221>  misc_feature
<222>  (4009)..(4089)
<223>  Sequence encoding 3x hemagglutinin (HA) tag

<400>  5
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgcc       60

accagatcct tcatcctgaa gatcgagccc aacgaggaag tgaagaaagg cctctggaaa      120

acccacgagg tgctgaacca cggaatcgcc tactacatga atatcctgaa gctgatccgg      180

caagaggcca tctacgagca ccacgagcag gaccccaaga atcccaagaa ggtgtccaag      240

gccgagatcc aggccgagct gtgggatttc gtgctgaaga tgcagaagtg caacagcttc      300

acacacgagg tggacaagga cgaggtgttc aacatcctga gagagctgta cgaggaactg      360

gtgcccagca gcgtggaaaa gaagggcgaa gccaaccagc tgagcaacaa gtttctgtac      420

cctctggtgg accccaacag ccagtctgga aagggaacag ccagcagcgg cagaaagccc      480

agatggtaca acctgaagat tgccggcgat ccctcctggg aagaagagaa gaagaagtgg      540

gaagaagata agaaaaagga cccgctggcc aagatcctgg gcaagctggc tgagtacgga      600

ctgatccctc tgttcatccc ctacaccgac agcaacgagc ccatcgtgaa agaaatcaag      660

tggatggaaa agtcccggaa ccagagcgtg cggcggctgg ataaggacat gttcattcag      720

gccctggaac ggttcctgag ctgggagagc tggaacctga aagtgaaaga ggaatacgag      780

aaggtcgaga aagagtacaa gaccctggaa gagaggatca aaggaggctc tggaggaagc      840

tccgaagtcg agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg      900

gctcgagatg aacgcgaggt gcccgtgggg gcagtactcg tgctcaacaa tcgcgtaatc      960

ggcgaaggtt ggaatagggc aatcggactc cacgacccca ctgcacatgc ggaaatcatg     1020

gcccttcgac agggagggct tgtgatgcag aattatcgac tttatgatgc gacgctgtac     1080

gtcacgtttg aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga     1140

gttgtattcg gtgttcgcaa cgccaagacg ggtgccgcag gttcactgat ggacgtgctg     1200

catcatccag gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt     1260

gcggcgctgt tgtgtcgttt ttttcgcatg cccaggcggg tctttaacgc ccagaaaaaa     1320

gcacaatcct ctactgacgg ctcttctgga tctgaaacac ctggcacaag cgagagcgcc     1380

acccctgaga gctctggcga ggacatccag gctctgaagg ctctggaaca gtatgagaaa     1440

gagcggcaag aacagctgct gcgggacacc ctgaacacca acgagtaccg gctgagcaag     1500

agaggcctta gaggctggcg ggaaatcatc cagaaatggc tgaaaatgga cgagaacgag     1560

ccctccgaga agtacctgga agtgttcaag gactaccagc ggaagcaccc tagagaggcc     1620

ggcgattaca gcgtgtacga gttcctgtcc aagaaagaga accacttcat ctggcggaat     1680

caccctgagt acccctacct gtacgccacc ttctgcgaga tcgacaagaa aaagaaggac     1740

gccaagcagc aggccacctt cacactggcc gatcctatca atcaccctct gtgggtccga     1800

ttcgaggaaa gaagcggcag caacctgaac aagtacagaa tcctgaccga gcagctgcac     1860

accgagaagc tgaagaaaaa gctgacagtg cagctggacc ggctgatcta ccctacagaa     1920

tctggcggct gggaagagaa gggcaaagtg gacattgtgc tgctgcccag ccggcagttc     1980

tacaaccaga tcttcctgga catcgaggaa aagggcaagc acgccttcac ctacaaggat     2040

gagagcatca agttccctct gaagggcaca ctcggcggag ccagagtgca gttcgacaga     2100

gatcacctga gaagataccc tcacaaggtg gaaagcggca acgtgggcag aatctacttc     2160

aacatgaccg tgaacatcga gcctacagag tccccagtgt ccaagtctct gaagatccac     2220

cgggacgact tccccaaggt ggtcaacttc aagcccaaag aactgaccga gtggatcaag     2280

gacagcaagg gcaagaaact gaagtccggc atcgagtccc tggaaatcgg cctgagagtg     2340

atgagcatcg acctgggaca gagacaggcc gctgccgcct ctattttcga ggtggtggat     2400

cagaagcccg acatcgaagg caagctgttt ttcccaatca agggcaccga gctgtatgcc     2460

gtgcacagag ccagcttcaa catcaagctg cccggcgaga cactggtcaa gagcagagaa     2520

gtgctgcgga aggccagaga ggacaatctg aaactgatga accagaagct caacttcctg     2580

cggaacgtgc tgcacttcca gcagttcgag gacatcaccg agagagagaa gcgggtcacc     2640

aagtggatca gcagacaaga gaacagcgac gtgcccctgg tgtaccagga tgagctgatc     2700

cagatccgcg agctgatgta caagccttac aaggactggg tcgccttcct gaagcagctc     2760

cacaagagac tggaagtcga gatcggcaaa gaagtgaagc actggcggaa gtccctgagc     2820

gacggaagaa agggcctgta cggcatctcc ctgaagaaca tcgacgagat cgatcggacc     2880

cggaagttcc tgctgagatg gtccctgagg cctaccgaac ctggcgaagt gcgtagactg     2940

gaacccggcc agagattcgc catcgaccag ctgaatcacc tgaacgccct gaaagaagat     3000

cggctgaaga agatggccaa caccatcatc atgcacgccc tgggctactg ctacgacgtg     3060

cggaagaaga aatggcaggc taagaacccc gcctgccaga tcatcctgtt cgaggatctg     3120

agcaactaca acccctacga ggaaaggtcc cgcttcgaga acagcaagct catgaagtgg     3180

tccagacgcg agatccccag acaggttgca ctgcagggcg agatctatgg cctgcaagtg     3240

ggagaagtgg gcgctcagtt cagcagcaga ttccacgcca agacaggcag ccctggcatc     3300

agatgtagcg tcgtgaccaa agagaagctg caggacaatc ggttcttcaa gaatctgcag     3360

agagagggca gactgaccct ggacaaaatc gccgtgctga aagagggcga tctgtaccca     3420

gacaaaggcg gcgagaagtt catcagcctg agcaaggatc ggaagtgcgt gaccacacac     3480

gccgacatca acgccgctca gaacctgcag aagcggttct ggacaagaac ccacggcttc     3540

tacaaggtgt actgcaaggc ctaccaggtg gacggccaga ccgtgtacat ccctgagagc     3600

aaggaccaga agcagaagat catcgaagag ttcggcgagg gctacttcat tctgaaggac     3660

ggggtgtacg aatgggtcaa cgccggcaag ctgaaaatca agaagggcag ctccaagcag     3720

agcagcagcg agctggtgga tagcgacatc ctgaaagaca gcttcgacct ggcctccgag     3780

ctgaaaggcg aaaagctgat gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac     3840

aaatggatgg ccgctggcgt gttcttcgga aagctggaac gcatcctgat cagcaagctg     3900

accaaccagt actccatcag caccatcgag gacgacagca gcaagcagtc tatgaaaagg     3960

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat     4020

gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc     4080

gactatgcct aa                                                         4092


<210>  6
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at K255 polypeptide sequence

<400>  6

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
                165                 170                 175     


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
            180                 185                 190         


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
        195                 200                 205             


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
    210                 215                 220                 


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
225                 230                 235                 240 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
                245                 250                 255     


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
            260                 265                 270         


Gly Gly Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr Trp 
        275                 280                 285             


Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu 
    290                 295                 300                 


Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu 
305                 310                 315                 320 


Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu 
                325                 330                 335     


Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu 
            340                 345                 350         


Tyr Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala 
        355                 360                 365             


Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg 
    370                 375                 380                 


Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His His 
385                 390                 395                 400 


Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp 
                405                 410                 415     


Glu Cys Ala Ala Leu Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val 
            420                 425                 430         


Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Gly Ser Ser Gly 
        435                 440                 445             


Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly 
    450                 455                 460                 


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
465                 470                 475                 480 


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
                485                 490                 495     


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
            500                 505                 510         


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
        515                 520                 525             


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
    530                 535                 540                 


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
545                 550                 555                 560 


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
                565                 570                 575     


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
            580                 585                 590         


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
        595                 600                 605             


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
    610                 615                 620                 


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
625                 630                 635                 640 


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
                645                 650                 655     


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
            660                 665                 670         


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
        675                 680                 685             


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
    690                 695                 700                 


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
705                 710                 715                 720 


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
                725                 730                 735     


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
            740                 745                 750         


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
        755                 760                 765             


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
    770                 775                 780                 


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
785                 790                 795                 800 


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
                805                 810                 815     


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
            820                 825                 830         


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
        835                 840                 845             


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
    850                 855                 860                 


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
865                 870                 875                 880 


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
                885                 890                 895     


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
            900                 905                 910         


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
        915                 920                 925             


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
    930                 935                 940                 


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
945                 950                 955                 960 


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
                965                 970                 975     


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
            980                 985                 990         


Asn Ala Leu Lys Glu Asp Arg Leu  Lys Lys Met Ala Asn  Thr Ile Ile 
        995                 1000                 1005             


Met His  Ala Leu Gly Tyr Cys  Tyr Asp Val Arg Lys  Lys Lys Trp 
    1010                 1015                 1020             


Gln Ala  Lys Asn Pro Ala Cys  Gln Ile Ile Leu Phe  Glu Asp Leu 
    1025                 1030                 1035             


Ser Asn  Tyr Asn Pro Tyr Glu  Glu Arg Ser Arg Phe  Glu Asn Ser 
    1040                 1045                 1050             


Lys Leu  Met Lys Trp Ser Arg  Arg Glu Ile Pro Arg  Gln Val Ala 
    1055                 1060                 1065             


Leu Gln  Gly Glu Ile Tyr Gly  Leu Gln Val Gly Glu  Val Gly Ala 
    1070                 1075                 1080             


Gln Phe  Ser Ser Arg Phe His  Ala Lys Thr Gly Ser  Pro Gly Ile 
    1085                 1090                 1095             


Arg Cys  Ser Val Val Thr Lys  Glu Lys Leu Gln Asp  Asn Arg Phe 
    1100                 1105                 1110             


Phe Lys  Asn Leu Gln Arg Glu  Gly Arg Leu Thr Leu  Asp Lys Ile 
    1115                 1120                 1125             


Ala Val  Leu Lys Glu Gly Asp  Leu Tyr Pro Asp Lys  Gly Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ser Leu Ser Lys  Asp Arg Lys Cys Val  Thr Thr His 
    1145                 1150                 1155             


Ala Asp  Ile Asn Ala Ala Gln  Asn Leu Gln Lys Arg  Phe Trp Thr 
    1160                 1165                 1170             


Arg Thr  His Gly Phe Tyr Lys  Val Tyr Cys Lys Ala  Tyr Gln Val 
    1175                 1180                 1185             


Asp Gly  Gln Thr Val Tyr Ile  Pro Glu Ser Lys Asp  Gln Lys Gln 
    1190                 1195                 1200             


Lys Ile  Ile Glu Glu Phe Gly  Glu Gly Tyr Phe Ile  Leu Lys Asp 
    1205                 1210                 1215             


Gly Val  Tyr Glu Trp Val Asn  Ala Gly Lys Leu Lys  Ile Lys Lys 
    1220                 1225                 1230             


Gly Ser  Ser Lys Gln Ser Ser  Ser Glu Leu Val Asp  Ser Asp Ile 
    1235                 1240                 1245             


Leu Lys  Asp Ser Phe Asp Leu  Ala Ser Glu Leu Lys  Gly Glu Lys 
    1250                 1255                 1260             


Leu Met  Leu Tyr Arg Asp Pro  Ser Gly Asn Val Phe  Pro Ser Asp 
    1265                 1270                 1275             


Lys Trp  Met Ala Ala Gly Val  Phe Phe Gly Lys Leu  Glu Arg Ile 
    1280                 1285                 1290             


Leu Ile  Ser Lys Leu Thr Asn  Gln Tyr Ser Ile Ser  Thr Ile Glu 
    1295                 1300                 1305             


Asp Asp  Ser Ser Lys Gln Ser  Met Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Gly Ser Tyr  Pro Tyr Asp 
    1325                 1330                 1335             


Val Pro  Asp Tyr Ala Tyr Pro  Tyr Asp Val Pro Asp  Tyr Ala Tyr 
    1340                 1345                 1350             


Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1355                 1360     


<210>  7
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at D306 polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (7)..(57)
<223>  Sequence encoding N-terminal nuclear localization signal (NLS)

<220>
<221>  misc_feature
<222>  (58)..(975)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (976)..(993)
<223>  Sequence encoding a linker

<220>
<221>  misc_feature
<222>  (994)..(1491)
<223>  Sequence encoding TadA*8

<220>
<221>  misc_feature
<222>  (1492)..(1551)
<223>  Sequence encoding Xten20 linker

<220>
<221>  misc_feature
<222>  (1552)..(3954)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3955)..(4002)
<223>  Sequence encoding C-terminal NLS

<220>
<221>  misc_feature
<222>  (4003)..(4008)
<223>  Sequence encoding a GS linker

<220>
<221>  misc_feature
<222>  (4009)..(4089)
<223>  Sequence encoding 3x hemagglutinin (HA) tag

<400>  7
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgcc       60

accagatcct tcatcctgaa gatcgagccc aacgaggaag tgaagaaagg cctctggaaa      120

acccacgagg tgctgaacca cggaatcgcc tactacatga atatcctgaa gctgatccgg      180

caagaggcca tctacgagca ccacgagcag gaccccaaga atcccaagaa ggtgtccaag      240

gccgagatcc aggccgagct gtgggatttc gtgctgaaga tgcagaagtg caacagcttc      300

acacacgagg tggacaagga cgaggtgttc aacatcctga gagagctgta cgaggaactg      360

gtgcccagca gcgtggaaaa gaagggcgaa gccaaccagc tgagcaacaa gtttctgtac      420

cctctggtgg accccaacag ccagtctgga aagggaacag ccagcagcgg cagaaagccc      480

agatggtaca acctgaagat tgccggcgat ccctcctggg aagaagagaa gaagaagtgg      540

gaagaagata agaaaaagga cccgctggcc aagatcctgg gcaagctggc tgagtacgga      600

ctgatccctc tgttcatccc ctacaccgac agcaacgagc ccatcgtgaa agaaatcaag      660

tggatggaaa agtcccggaa ccagagcgtg cggcggctgg ataaggacat gttcattcag      720

gccctggaac ggttcctgag ctgggagagc tggaacctga aagtgaaaga ggaatacgag      780

aaggtcgaga aagagtacaa gaccctggaa gagaggatca aagaggacat ccaggctctg      840

aaggctctgg aacagtatga gaaagagcgg caagaacagc tgctgcggga caccctgaac      900

accaacgagt accggctgag caagagaggc cttagaggct ggcgggaaat catccagaaa      960

tggctgaaaa tggacggagg ctctggagga agctccgaag tcgagttttc ccatgagtac     1020

tggatgagac acgcattgac tctcgcaaag agggctcgag atgaacgcga ggtgcccgtg     1080

ggggcagtac tcgtgctcaa caatcgcgta atcggcgaag gttggaatag ggcaatcgga     1140

ctccacgacc ccactgcaca tgcggaaatc atggcccttc gacagggagg gcttgtgatg     1200

cagaattatc gactttatga tgcgacgctg tacgtcacgt ttgaaccttg cgtaatgtgc     1260

gcgggagcta tgattcactc ccgcattgga cgagttgtat tcggtgttcg caacgccaag     1320

acgggtgccg caggttcact gatggacgtg ctgcatcatc caggcatgaa ccaccgggta     1380

gaaatcacag aaggcatatt ggcggacgaa tgtgcggcgc tgttgtgtcg tttttttcgc     1440

atgcccaggc gggtctttaa cgcccagaaa aaagcacaat cctctactga cggctcttct     1500

ggatctgaaa cacctggcac aagcgagagc gccacccctg agagctctgg cgagaacgag     1560

ccctccgaga agtacctgga agtgttcaag gactaccagc ggaagcaccc tagagaggcc     1620

ggcgattaca gcgtgtacga gttcctgtcc aagaaagaga accacttcat ctggcggaat     1680

caccctgagt acccctacct gtacgccacc ttctgcgaga tcgacaagaa aaagaaggac     1740

gccaagcagc aggccacctt cacactggcc gatcctatca atcaccctct gtgggtccga     1800

ttcgaggaaa gaagcggcag caacctgaac aagtacagaa tcctgaccga gcagctgcac     1860

accgagaagc tgaagaaaaa gctgacagtg cagctggacc ggctgatcta ccctacagaa     1920

tctggcggct gggaagagaa gggcaaagtg gacattgtgc tgctgcccag ccggcagttc     1980

tacaaccaga tcttcctgga catcgaggaa aagggcaagc acgccttcac ctacaaggat     2040

gagagcatca agttccctct gaagggcaca ctcggcggag ccagagtgca gttcgacaga     2100

gatcacctga gaagataccc tcacaaggtg gaaagcggca acgtgggcag aatctacttc     2160

aacatgaccg tgaacatcga gcctacagag tccccagtgt ccaagtctct gaagatccac     2220

cgggacgact tccccaaggt ggtcaacttc aagcccaaag aactgaccga gtggatcaag     2280

gacagcaagg gcaagaaact gaagtccggc atcgagtccc tggaaatcgg cctgagagtg     2340

atgagcatcg acctgggaca gagacaggcc gctgccgcct ctattttcga ggtggtggat     2400

cagaagcccg acatcgaagg caagctgttt ttcccaatca agggcaccga gctgtatgcc     2460

gtgcacagag ccagcttcaa catcaagctg cccggcgaga cactggtcaa gagcagagaa     2520

gtgctgcgga aggccagaga ggacaatctg aaactgatga accagaagct caacttcctg     2580

cggaacgtgc tgcacttcca gcagttcgag gacatcaccg agagagagaa gcgggtcacc     2640

aagtggatca gcagacaaga gaacagcgac gtgcccctgg tgtaccagga tgagctgatc     2700

cagatccgcg agctgatgta caagccttac aaggactggg tcgccttcct gaagcagctc     2760

cacaagagac tggaagtcga gatcggcaaa gaagtgaagc actggcggaa gtccctgagc     2820

gacggaagaa agggcctgta cggcatctcc ctgaagaaca tcgacgagat cgatcggacc     2880

cggaagttcc tgctgagatg gtccctgagg cctaccgaac ctggcgaagt gcgtagactg     2940

gaacccggcc agagattcgc catcgaccag ctgaatcacc tgaacgccct gaaagaagat     3000

cggctgaaga agatggccaa caccatcatc atgcacgccc tgggctactg ctacgacgtg     3060

cggaagaaga aatggcaggc taagaacccc gcctgccaga tcatcctgtt cgaggatctg     3120

agcaactaca acccctacga ggaaaggtcc cgcttcgaga acagcaagct catgaagtgg     3180

tccagacgcg agatccccag acaggttgca ctgcagggcg agatctatgg cctgcaagtg     3240

ggagaagtgg gcgctcagtt cagcagcaga ttccacgcca agacaggcag ccctggcatc     3300

agatgtagcg tcgtgaccaa agagaagctg caggacaatc ggttcttcaa gaatctgcag     3360

agagagggca gactgaccct ggacaaaatc gccgtgctga aagagggcga tctgtaccca     3420

gacaaaggcg gcgagaagtt catcagcctg agcaaggatc ggaagtgcgt gaccacacac     3480

gccgacatca acgccgctca gaacctgcag aagcggttct ggacaagaac ccacggcttc     3540

tacaaggtgt actgcaaggc ctaccaggtg gacggccaga ccgtgtacat ccctgagagc     3600

aaggaccaga agcagaagat catcgaagag ttcggcgagg gctacttcat tctgaaggac     3660

ggggtgtacg aatgggtcaa cgccggcaag ctgaaaatca agaagggcag ctccaagcag     3720

agcagcagcg agctggtgga tagcgacatc ctgaaagaca gcttcgacct ggcctccgag     3780

ctgaaaggcg aaaagctgat gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac     3840

aaatggatgg ccgctggcgt gttcttcgga aagctggaac gcatcctgat cagcaagctg     3900

accaaccagt actccatcag caccatcgag gacgacagca gcaagcagtc tatgaaaagg     3960

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat     4020

gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc     4080

gactatgcct aa                                                         4092


<210>  8
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at D306 polypeptide sequence

<400>  8

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
                165                 170                 175     


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
            180                 185                 190         


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
        195                 200                 205             


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
    210                 215                 220                 


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
225                 230                 235                 240 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
                245                 250                 255     


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
            260                 265                 270         


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
        275                 280                 285             


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
    290                 295                 300                 


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
305                 310                 315                 320 


Lys Met Asp Gly Gly Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His 
                325                 330                 335     


Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp 
            340                 345                 350         


Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val 
        355                 360                 365             


Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala 
    370                 375                 380                 


His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn 
385                 390                 395                 400 


Tyr Arg Leu Tyr Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val 
                405                 410                 415     


Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe 
            420                 425                 430         


Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val 
        435                 440                 445             


Leu His His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile 
    450                 455                 460                 


Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Arg Phe Phe Arg Met Pro 
465                 470                 475                 480 


Arg Arg Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Gly 
                485                 490                 495     


Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
            500                 505                 510         


Ser Ser Gly Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
        515                 520                 525             


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
    530                 535                 540                 


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
545                 550                 555                 560 


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
                565                 570                 575     


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
            580                 585                 590         


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
        595                 600                 605             


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
    610                 615                 620                 


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
625                 630                 635                 640 


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
                645                 650                 655     


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
            660                 665                 670         


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
        675                 680                 685             


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
    690                 695                 700                 


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
705                 710                 715                 720 


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
                725                 730                 735     


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
            740                 745                 750         


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
        755                 760                 765             


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
    770                 775                 780                 


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
785                 790                 795                 800 


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
                805                 810                 815     


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
            820                 825                 830         


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
        835                 840                 845             


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
    850                 855                 860                 


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
865                 870                 875                 880 


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
                885                 890                 895     


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
            900                 905                 910         


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
        915                 920                 925             


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
    930                 935                 940                 


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
945                 950                 955                 960 


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
                965                 970                 975     


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
            980                 985                 990         


Asn Ala Leu Lys Glu Asp Arg Leu  Lys Lys Met Ala Asn  Thr Ile Ile 
        995                 1000                 1005             


Met His  Ala Leu Gly Tyr Cys  Tyr Asp Val Arg Lys  Lys Lys Trp 
    1010                 1015                 1020             


Gln Ala  Lys Asn Pro Ala Cys  Gln Ile Ile Leu Phe  Glu Asp Leu 
    1025                 1030                 1035             


Ser Asn  Tyr Asn Pro Tyr Glu  Glu Arg Ser Arg Phe  Glu Asn Ser 
    1040                 1045                 1050             


Lys Leu  Met Lys Trp Ser Arg  Arg Glu Ile Pro Arg  Gln Val Ala 
    1055                 1060                 1065             


Leu Gln  Gly Glu Ile Tyr Gly  Leu Gln Val Gly Glu  Val Gly Ala 
    1070                 1075                 1080             


Gln Phe  Ser Ser Arg Phe His  Ala Lys Thr Gly Ser  Pro Gly Ile 
    1085                 1090                 1095             


Arg Cys  Ser Val Val Thr Lys  Glu Lys Leu Gln Asp  Asn Arg Phe 
    1100                 1105                 1110             


Phe Lys  Asn Leu Gln Arg Glu  Gly Arg Leu Thr Leu  Asp Lys Ile 
    1115                 1120                 1125             


Ala Val  Leu Lys Glu Gly Asp  Leu Tyr Pro Asp Lys  Gly Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ser Leu Ser Lys  Asp Arg Lys Cys Val  Thr Thr His 
    1145                 1150                 1155             


Ala Asp  Ile Asn Ala Ala Gln  Asn Leu Gln Lys Arg  Phe Trp Thr 
    1160                 1165                 1170             


Arg Thr  His Gly Phe Tyr Lys  Val Tyr Cys Lys Ala  Tyr Gln Val 
    1175                 1180                 1185             


Asp Gly  Gln Thr Val Tyr Ile  Pro Glu Ser Lys Asp  Gln Lys Gln 
    1190                 1195                 1200             


Lys Ile  Ile Glu Glu Phe Gly  Glu Gly Tyr Phe Ile  Leu Lys Asp 
    1205                 1210                 1215             


Gly Val  Tyr Glu Trp Val Asn  Ala Gly Lys Leu Lys  Ile Lys Lys 
    1220                 1225                 1230             


Gly Ser  Ser Lys Gln Ser Ser  Ser Glu Leu Val Asp  Ser Asp Ile 
    1235                 1240                 1245             


Leu Lys  Asp Ser Phe Asp Leu  Ala Ser Glu Leu Lys  Gly Glu Lys 
    1250                 1255                 1260             


Leu Met  Leu Tyr Arg Asp Pro  Ser Gly Asn Val Phe  Pro Ser Asp 
    1265                 1270                 1275             


Lys Trp  Met Ala Ala Gly Val  Phe Phe Gly Lys Leu  Glu Arg Ile 
    1280                 1285                 1290             


Leu Ile  Ser Lys Leu Thr Asn  Gln Tyr Ser Ile Ser  Thr Ile Glu 
    1295                 1300                 1305             


Asp Asp  Ser Ser Lys Gln Ser  Met Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Gly Ser Tyr  Pro Tyr Asp 
    1325                 1330                 1335             


Val Pro  Asp Tyr Ala Tyr Pro  Tyr Asp Val Pro Asp  Tyr Ala Tyr 
    1340                 1345                 1350             


Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1355                 1360     


<210>  9
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at D980 polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (7)..(57)
<223>  Sequence encoding N-terminal nuclear localization signal (NLS)

<220>
<221>  misc_feature
<222>  (58)..(2997)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (2998)..(3015)
<223>  Sequence encoding a linker

<220>
<221>  misc_feature
<222>  (3016)..(3513)
<223>  Sequence encoding TadA*8

<220>
<221>  misc_feature
<222>  (3514)..(3573)
<223>  Sequence encoding Xten20 linker

<220>
<221>  misc_feature
<222>  (3574)..(3954)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3955)..(4002)
<223>  Sequence encoding C-terminal NLS

<220>
<221>  misc_feature
<222>  (4003)..(4008)
<223>  Sequence encoding a GS linker

<220>
<221>  misc_feature
<222>  (4009)..(4089)
<223>  Sequence encoding 3x hemagglutinin (HA) tag

<400>  9
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgcc       60

accagatcct tcatcctgaa gatcgagccc aacgaggaag tgaagaaagg cctctggaaa      120

acccacgagg tgctgaacca cggaatcgcc tactacatga atatcctgaa gctgatccgg      180

caagaggcca tctacgagca ccacgagcag gaccccaaga atcccaagaa ggtgtccaag      240

gccgagatcc aggccgagct gtgggatttc gtgctgaaga tgcagaagtg caacagcttc      300

acacacgagg tggacaagga cgaggtgttc aacatcctga gagagctgta cgaggaactg      360

gtgcccagca gcgtggaaaa gaagggcgaa gccaaccagc tgagcaacaa gtttctgtac      420

cctctggtgg accccaacag ccagtctgga aagggaacag ccagcagcgg cagaaagccc      480

agatggtaca acctgaagat tgccggcgat ccctcctggg aagaagagaa gaagaagtgg      540

gaagaagata agaaaaagga cccgctggcc aagatcctgg gcaagctggc tgagtacgga      600

ctgatccctc tgttcatccc ctacaccgac agcaacgagc ccatcgtgaa agaaatcaag      660

tggatggaaa agtcccggaa ccagagcgtg cggcggctgg ataaggacat gttcattcag      720

gccctggaac ggttcctgag ctgggagagc tggaacctga aagtgaaaga ggaatacgag      780

aaggtcgaga aagagtacaa gaccctggaa gagaggatca aagaggacat ccaggctctg      840

aaggctctgg aacagtatga gaaagagcgg caagaacagc tgctgcggga caccctgaac      900

accaacgagt accggctgag caagagaggc cttagaggct ggcgggaaat catccagaaa      960

tggctgaaaa tggacgagaa cgagccctcc gagaagtacc tggaagtgtt caaggactac     1020

cagcggaagc accctagaga ggccggcgat tacagcgtgt acgagttcct gtccaagaaa     1080

gagaaccact tcatctggcg gaatcaccct gagtacccct acctgtacgc caccttctgc     1140

gagatcgaca agaaaaagaa ggacgccaag cagcaggcca ccttcacact ggccgatcct     1200

atcaatcacc ctctgtgggt ccgattcgag gaaagaagcg gcagcaacct gaacaagtac     1260

agaatcctga ccgagcagct gcacaccgag aagctgaaga aaaagctgac agtgcagctg     1320

gaccggctga tctaccctac agaatctggc ggctgggaag agaagggcaa agtggacatt     1380

gtgctgctgc ccagccggca gttctacaac cagatcttcc tggacatcga ggaaaagggc     1440

aagcacgcct tcacctacaa ggatgagagc atcaagttcc ctctgaaggg cacactcggc     1500

ggagccagag tgcagttcga cagagatcac ctgagaagat accctcacaa ggtggaaagc     1560

ggcaacgtgg gcagaatcta cttcaacatg accgtgaaca tcgagcctac agagtcccca     1620

gtgtccaagt ctctgaagat ccaccgggac gacttcccca aggtggtcaa cttcaagccc     1680

aaagaactga ccgagtggat caaggacagc aagggcaaga aactgaagtc cggcatcgag     1740

tccctggaaa tcggcctgag agtgatgagc atcgacctgg gacagagaca ggccgctgcc     1800

gcctctattt tcgaggtggt ggatcagaag cccgacatcg aaggcaagct gtttttccca     1860

atcaagggca ccgagctgta tgccgtgcac agagccagct tcaacatcaa gctgcccggc     1920

gagacactgg tcaagagcag agaagtgctg cggaaggcca gagaggacaa tctgaaactg     1980

atgaaccaga agctcaactt cctgcggaac gtgctgcact tccagcagtt cgaggacatc     2040

accgagagag agaagcgggt caccaagtgg atcagcagac aagagaacag cgacgtgccc     2100

ctggtgtacc aggatgagct gatccagatc cgcgagctga tgtacaagcc ttacaaggac     2160

tgggtcgcct tcctgaagca gctccacaag agactggaag tcgagatcgg caaagaagtg     2220

aagcactggc ggaagtccct gagcgacgga agaaagggcc tgtacggcat ctccctgaag     2280

aacatcgacg agatcgatcg gacccggaag ttcctgctga gatggtccct gaggcctacc     2340

gaacctggcg aagtgcgtag actggaaccc ggccagagat tcgccatcga ccagctgaat     2400

cacctgaacg ccctgaaaga agatcggctg aagaagatgg ccaacaccat catcatgcac     2460

gccctgggct actgctacga cgtgcggaag aagaaatggc aggctaagaa ccccgcctgc     2520

cagatcatcc tgttcgagga tctgagcaac tacaacccct acgaggaaag gtcccgcttc     2580

gagaacagca agctcatgaa gtggtccaga cgcgagatcc ccagacaggt tgcactgcag     2640

ggcgagatct atggcctgca agtgggagaa gtgggcgctc agttcagcag cagattccac     2700

gccaagacag gcagccctgg catcagatgt agcgtcgtga ccaaagagaa gctgcaggac     2760

aatcggttct tcaagaatct gcagagagag ggcagactga ccctggacaa aatcgccgtg     2820

ctgaaagagg gcgatctgta cccagacaaa ggcggcgaga agttcatcag cctgagcaag     2880

gatcggaagt gcgtgaccac acacgccgac atcaacgccg ctcagaacct gcagaagcgg     2940

ttctggacaa gaacccacgg cttctacaag gtgtactgca aggcctacca ggtggacgga     3000

ggctctggag gaagctccga agtcgagttt tcccatgagt actggatgag acacgcattg     3060

actctcgcaa agagggctcg agatgaacgc gaggtgcccg tgggggcagt actcgtgctc     3120

aacaatcgcg taatcggcga aggttggaat agggcaatcg gactccacga ccccactgca     3180

catgcggaaa tcatggccct tcgacaggga gggcttgtga tgcagaatta tcgactttat     3240

gatgcgacgc tgtacgtcac gtttgaacct tgcgtaatgt gcgcgggagc tatgattcac     3300

tcccgcattg gacgagttgt attcggtgtt cgcaacgcca agacgggtgc cgcaggttca     3360

ctgatggacg tgctgcatca tccaggcatg aaccaccggg tagaaatcac agaaggcata     3420

ttggcggacg aatgtgcggc gctgttgtgt cgtttttttc gcatgcccag gcgggtcttt     3480

aacgcccaga aaaaagcaca atcctctact gacggctctt ctggatctga aacacctggc     3540

acaagcgaga gcgccacccc tgagagctct ggcggccaga ccgtgtacat ccctgagagc     3600

aaggaccaga agcagaagat catcgaagag ttcggcgagg gctacttcat tctgaaggac     3660

ggggtgtacg aatgggtcaa cgccggcaag ctgaaaatca agaagggcag ctccaagcag     3720

agcagcagcg agctggtgga tagcgacatc ctgaaagaca gcttcgacct ggcctccgag     3780

ctgaaaggcg aaaagctgat gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac     3840

aaatggatgg ccgctggcgt gttcttcgga aagctggaac gcatcctgat cagcaagctg     3900

accaaccagt actccatcag caccatcgag gacgacagca gcaagcagtc tatgaaaagg     3960

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat     4020

gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc     4080

gactatgcct aa                                                         4092


<210>  10
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at D980 polypeptide sequence

<400>  10

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
                165                 170                 175     


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
            180                 185                 190         


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
        195                 200                 205             


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
    210                 215                 220                 


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
225                 230                 235                 240 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
                245                 250                 255     


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
            260                 265                 270         


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
        275                 280                 285             


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
    290                 295                 300                 


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
305                 310                 315                 320 


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
                325                 330                 335     


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
            340                 345                 350         


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
        355                 360                 365             


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
    370                 375                 380                 


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
385                 390                 395                 400 


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
                405                 410                 415     


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
            420                 425                 430         


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
        435                 440                 445             


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
    450                 455                 460                 


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
465                 470                 475                 480 


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
                485                 490                 495     


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
            500                 505                 510         


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
        515                 520                 525             


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
    530                 535                 540                 


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
545                 550                 555                 560 


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
                565                 570                 575     


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
            580                 585                 590         


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
        595                 600                 605             


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
    610                 615                 620                 


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
625                 630                 635                 640 


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
                645                 650                 655     


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
            660                 665                 670         


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
        675                 680                 685             


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
    690                 695                 700                 


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
705                 710                 715                 720 


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
                725                 730                 735     


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
            740                 745                 750         


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
        755                 760                 765             


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
    770                 775                 780                 


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
785                 790                 795                 800 


Asn Ala Leu Lys Glu Asp Arg Leu Lys Lys Met Ala Asn Thr Ile Ile 
                805                 810                 815     


Met His Ala Leu Gly Tyr Cys Tyr Asp Val Arg Lys Lys Lys Trp Gln 
            820                 825                 830         


Ala Lys Asn Pro Ala Cys Gln Ile Ile Leu Phe Glu Asp Leu Ser Asn 
        835                 840                 845             


Tyr Asn Pro Tyr Glu Glu Arg Ser Arg Phe Glu Asn Ser Lys Leu Met 
    850                 855                 860                 


Lys Trp Ser Arg Arg Glu Ile Pro Arg Gln Val Ala Leu Gln Gly Glu 
865                 870                 875                 880 


Ile Tyr Gly Leu Gln Val Gly Glu Val Gly Ala Gln Phe Ser Ser Arg 
                885                 890                 895     


Phe His Ala Lys Thr Gly Ser Pro Gly Ile Arg Cys Ser Val Val Thr 
            900                 905                 910         


Lys Glu Lys Leu Gln Asp Asn Arg Phe Phe Lys Asn Leu Gln Arg Glu 
        915                 920                 925             


Gly Arg Leu Thr Leu Asp Lys Ile Ala Val Leu Lys Glu Gly Asp Leu 
    930                 935                 940                 


Tyr Pro Asp Lys Gly Gly Glu Lys Phe Ile Ser Leu Ser Lys Asp Arg 
945                 950                 955                 960 


Lys Cys Val Thr Thr His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln 
                965                 970                 975     


Lys Arg Phe Trp Thr Arg Thr His Gly Phe Tyr Lys Val Tyr Cys Lys 
            980                 985                 990         


Ala Tyr Gln Val Asp Gly Gly Ser  Gly Gly Ser Ser Glu  Val Glu Phe 
        995                 1000                 1005             


Ser His  Glu Tyr Trp Met Arg  His Ala Leu Thr Leu  Ala Lys Arg 
    1010                 1015                 1020             


Ala Arg  Asp Glu Arg Glu Val  Pro Val Gly Ala Val  Leu Val Leu 
    1025                 1030                 1035             


Asn Asn  Arg Val Ile Gly Glu  Gly Trp Asn Arg Ala  Ile Gly Leu 
    1040                 1045                 1050             


His Asp  Pro Thr Ala His Ala  Glu Ile Met Ala Leu  Arg Gln Gly 
    1055                 1060                 1065             


Gly Leu  Val Met Gln Asn Tyr  Arg Leu Tyr Asp Ala  Thr Leu Tyr 
    1070                 1075                 1080             


Val Thr  Phe Glu Pro Cys Val  Met Cys Ala Gly Ala  Met Ile His 
    1085                 1090                 1095             


Ser Arg  Ile Gly Arg Val Val  Phe Gly Val Arg Asn  Ala Lys Thr 
    1100                 1105                 1110             


Gly Ala  Ala Gly Ser Leu Met  Asp Val Leu His His  Pro Gly Met 
    1115                 1120                 1125             


Asn His  Arg Val Glu Ile Thr  Glu Gly Ile Leu Ala  Asp Glu Cys 
    1130                 1135                 1140             


Ala Ala  Leu Leu Cys Arg Phe  Phe Arg Met Pro Arg  Arg Val Phe 
    1145                 1150                 1155             


Asn Ala  Gln Lys Lys Ala Gln  Ser Ser Thr Asp Gly  Ser Ser Gly 
    1160                 1165                 1170             


Ser Glu  Thr Pro Gly Thr Ser  Glu Ser Ala Thr Pro  Glu Ser Ser 
    1175                 1180                 1185             


Gly Gly  Gln Thr Val Tyr Ile  Pro Glu Ser Lys Asp  Gln Lys Gln 
    1190                 1195                 1200             


Lys Ile  Ile Glu Glu Phe Gly  Glu Gly Tyr Phe Ile  Leu Lys Asp 
    1205                 1210                 1215             


Gly Val  Tyr Glu Trp Val Asn  Ala Gly Lys Leu Lys  Ile Lys Lys 
    1220                 1225                 1230             


Gly Ser  Ser Lys Gln Ser Ser  Ser Glu Leu Val Asp  Ser Asp Ile 
    1235                 1240                 1245             


Leu Lys  Asp Ser Phe Asp Leu  Ala Ser Glu Leu Lys  Gly Glu Lys 
    1250                 1255                 1260             


Leu Met  Leu Tyr Arg Asp Pro  Ser Gly Asn Val Phe  Pro Ser Asp 
    1265                 1270                 1275             


Lys Trp  Met Ala Ala Gly Val  Phe Phe Gly Lys Leu  Glu Arg Ile 
    1280                 1285                 1290             


Leu Ile  Ser Lys Leu Thr Asn  Gln Tyr Ser Ile Ser  Thr Ile Glu 
    1295                 1300                 1305             


Asp Asp  Ser Ser Lys Gln Ser  Met Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Gly Ser Tyr  Pro Tyr Asp 
    1325                 1330                 1335             


Val Pro  Asp Tyr Ala Tyr Pro  Tyr Asp Val Pro Asp  Tyr Ala Tyr 
    1340                 1345                 1350             


Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1355                 1360     


<210>  11
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at K1019 polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (7)..(57)
<223>  Sequence encoding N-terminal nuclear localization signal (NLS)

<220>
<221>  misc_feature
<222>  (58)..(3114)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3115)..(3132)
<223>  Sequence encoding a linker

<220>
<221>  misc_feature
<222>  (3133)..(3630)
<223>  Sequence encoding TadA*8

<220>
<221>  misc_feature
<222>  (3631)..(3690)
<223>  Sequence encoding Xten20 linker

<220>
<221>  misc_feature
<222>  (3691)..(3954)
<223>  Sequence encoding BhCas12b

<220>
<221>  misc_feature
<222>  (3955)..(4002)
<223>  Sequence encoding C-terminal NLS

<220>
<221>  misc_feature
<222>  (4003)..(4008)
<223>  Sequence encoding GS linker

<220>
<221>  misc_feature
<222>  (4009)..(4089)
<223>  Sequence encoding 3x hemagglutinin (HA) tag

<400>  11
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgcc       60

accagatcct tcatcctgaa gatcgagccc aacgaggaag tgaagaaagg cctctggaaa      120

acccacgagg tgctgaacca cggaatcgcc tactacatga atatcctgaa gctgatccgg      180

caagaggcca tctacgagca ccacgagcag gaccccaaga atcccaagaa ggtgtccaag      240

gccgagatcc aggccgagct gtgggatttc gtgctgaaga tgcagaagtg caacagcttc      300

acacacgagg tggacaagga cgaggtgttc aacatcctga gagagctgta cgaggaactg      360

gtgcccagca gcgtggaaaa gaagggcgaa gccaaccagc tgagcaacaa gtttctgtac      420

cctctggtgg accccaacag ccagtctgga aagggaacag ccagcagcgg cagaaagccc      480

agatggtaca acctgaagat tgccggcgat ccctcctggg aagaagagaa gaagaagtgg      540

gaagaagata agaaaaagga cccgctggcc aagatcctgg gcaagctggc tgagtacgga      600

ctgatccctc tgttcatccc ctacaccgac agcaacgagc ccatcgtgaa agaaatcaag      660

tggatggaaa agtcccggaa ccagagcgtg cggcggctgg ataaggacat gttcattcag      720

gccctggaac ggttcctgag ctgggagagc tggaacctga aagtgaaaga ggaatacgag      780

aaggtcgaga aagagtacaa gaccctggaa gagaggatca aagaggacat ccaggctctg      840

aaggctctgg aacagtatga gaaagagcgg caagaacagc tgctgcggga caccctgaac      900

accaacgagt accggctgag caagagaggc cttagaggct ggcgggaaat catccagaaa      960

tggctgaaaa tggacgagaa cgagccctcc gagaagtacc tggaagtgtt caaggactac     1020

cagcggaagc accctagaga ggccggcgat tacagcgtgt acgagttcct gtccaagaaa     1080

gagaaccact tcatctggcg gaatcaccct gagtacccct acctgtacgc caccttctgc     1140

gagatcgaca agaaaaagaa ggacgccaag cagcaggcca ccttcacact ggccgatcct     1200

atcaatcacc ctctgtgggt ccgattcgag gaaagaagcg gcagcaacct gaacaagtac     1260

agaatcctga ccgagcagct gcacaccgag aagctgaaga aaaagctgac agtgcagctg     1320

gaccggctga tctaccctac agaatctggc ggctgggaag agaagggcaa agtggacatt     1380

gtgctgctgc ccagccggca gttctacaac cagatcttcc tggacatcga ggaaaagggc     1440

aagcacgcct tcacctacaa ggatgagagc atcaagttcc ctctgaaggg cacactcggc     1500

ggagccagag tgcagttcga cagagatcac ctgagaagat accctcacaa ggtggaaagc     1560

ggcaacgtgg gcagaatcta cttcaacatg accgtgaaca tcgagcctac agagtcccca     1620

gtgtccaagt ctctgaagat ccaccgggac gacttcccca aggtggtcaa cttcaagccc     1680

aaagaactga ccgagtggat caaggacagc aagggcaaga aactgaagtc cggcatcgag     1740

tccctggaaa tcggcctgag agtgatgagc atcgacctgg gacagagaca ggccgctgcc     1800

gcctctattt tcgaggtggt ggatcagaag cccgacatcg aaggcaagct gtttttccca     1860

atcaagggca ccgagctgta tgccgtgcac agagccagct tcaacatcaa gctgcccggc     1920

gagacactgg tcaagagcag agaagtgctg cggaaggcca gagaggacaa tctgaaactg     1980

atgaaccaga agctcaactt cctgcggaac gtgctgcact tccagcagtt cgaggacatc     2040

accgagagag agaagcgggt caccaagtgg atcagcagac aagagaacag cgacgtgccc     2100

ctggtgtacc aggatgagct gatccagatc cgcgagctga tgtacaagcc ttacaaggac     2160

tgggtcgcct tcctgaagca gctccacaag agactggaag tcgagatcgg caaagaagtg     2220

aagcactggc ggaagtccct gagcgacgga agaaagggcc tgtacggcat ctccctgaag     2280

aacatcgacg agatcgatcg gacccggaag ttcctgctga gatggtccct gaggcctacc     2340

gaacctggcg aagtgcgtag actggaaccc ggccagagat tcgccatcga ccagctgaat     2400

cacctgaacg ccctgaaaga agatcggctg aagaagatgg ccaacaccat catcatgcac     2460

gccctgggct actgctacga cgtgcggaag aagaaatggc aggctaagaa ccccgcctgc     2520

cagatcatcc tgttcgagga tctgagcaac tacaacccct acgaggaaag gtcccgcttc     2580

gagaacagca agctcatgaa gtggtccaga cgcgagatcc ccagacaggt tgcactgcag     2640

ggcgagatct atggcctgca agtgggagaa gtgggcgctc agttcagcag cagattccac     2700

gccaagacag gcagccctgg catcagatgt agcgtcgtga ccaaagagaa gctgcaggac     2760

aatcggttct tcaagaatct gcagagagag ggcagactga ccctggacaa aatcgccgtg     2820

ctgaaagagg gcgatctgta cccagacaaa ggcggcgaga agttcatcag cctgagcaag     2880

gatcggaagt gcgtgaccac acacgccgac atcaacgccg ctcagaacct gcagaagcgg     2940

ttctggacaa gaacccacgg cttctacaag gtgtactgca aggcctacca ggtggacggc     3000

cagaccgtgt acatccctga gagcaaggac cagaagcaga agatcatcga agagttcggc     3060

gagggctact tcattctgaa ggacggggtg tacgaatggg tcaacgccgg caagggaggc     3120

tctggaggaa gctccgaagt cgagttttcc catgagtact ggatgagaca cgcattgact     3180

ctcgcaaaga gggctcgaga tgaacgcgag gtgcccgtgg gggcagtact cgtgctcaac     3240

aatcgcgtaa tcggcgaagg ttggaatagg gcaatcggac tccacgaccc cactgcacat     3300

gcggaaatca tggcccttcg acagggaggg cttgtgatgc agaattatcg actttatgat     3360

gcgacgctgt acgtcacgtt tgaaccttgc gtaatgtgcg cgggagctat gattcactcc     3420

cgcattggac gagttgtatt cggtgttcgc aacgccaaga cgggtgccgc aggttcactg     3480

atggacgtgc tgcatcatcc aggcatgaac caccgggtag aaatcacaga aggcatattg     3540

gcggacgaat gtgcggcgct gttgtgtcgt ttttttcgca tgcccaggcg ggtctttaac     3600

gcccagaaaa aagcacaatc ctctactgac ggctcttctg gatctgaaac acctggcaca     3660

agcgagagcg ccacccctga gagctctggc ctgaaaatca agaagggcag ctccaagcag     3720

agcagcagcg agctggtgga tagcgacatc ctgaaagaca gcttcgacct ggcctccgag     3780

ctgaaaggcg aaaagctgat gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac     3840

aaatggatgg ccgctggcgt gttcttcgga aagctggaac gcatcctgat cagcaagctg     3900

accaaccagt actccatcag caccatcgag gacgacagca gcaagcagtc tatgaaaagg     3960

ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat     4020

gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc     4080

gactatgcct aa                                                         4092


<210>  12
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: BhCas12b GGSGGS-ABE8-Xten20 
       at K1019 polypeptide sequence

<400>  12

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
                165                 170                 175     


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
            180                 185                 190         


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
        195                 200                 205             


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
    210                 215                 220                 


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
225                 230                 235                 240 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
                245                 250                 255     


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
            260                 265                 270         


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
        275                 280                 285             


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
    290                 295                 300                 


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
305                 310                 315                 320 


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
                325                 330                 335     


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
            340                 345                 350         


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
        355                 360                 365             


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
    370                 375                 380                 


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
385                 390                 395                 400 


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
                405                 410                 415     


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
            420                 425                 430         


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
        435                 440                 445             


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
    450                 455                 460                 


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
465                 470                 475                 480 


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
                485                 490                 495     


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
            500                 505                 510         


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
        515                 520                 525             


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
    530                 535                 540                 


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
545                 550                 555                 560 


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
                565                 570                 575     


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
            580                 585                 590         


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
        595                 600                 605             


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
    610                 615                 620                 


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
625                 630                 635                 640 


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
                645                 650                 655     


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
            660                 665                 670         


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
        675                 680                 685             


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
    690                 695                 700                 


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
705                 710                 715                 720 


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
                725                 730                 735     


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
            740                 745                 750         


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
        755                 760                 765             


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
    770                 775                 780                 


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
785                 790                 795                 800 


Asn Ala Leu Lys Glu Asp Arg Leu Lys Lys Met Ala Asn Thr Ile Ile 
                805                 810                 815     


Met His Ala Leu Gly Tyr Cys Tyr Asp Val Arg Lys Lys Lys Trp Gln 
            820                 825                 830         


Ala Lys Asn Pro Ala Cys Gln Ile Ile Leu Phe Glu Asp Leu Ser Asn 
        835                 840                 845             


Tyr Asn Pro Tyr Glu Glu Arg Ser Arg Phe Glu Asn Ser Lys Leu Met 
    850                 855                 860                 


Lys Trp Ser Arg Arg Glu Ile Pro Arg Gln Val Ala Leu Gln Gly Glu 
865                 870                 875                 880 


Ile Tyr Gly Leu Gln Val Gly Glu Val Gly Ala Gln Phe Ser Ser Arg 
                885                 890                 895     


Phe His Ala Lys Thr Gly Ser Pro Gly Ile Arg Cys Ser Val Val Thr 
            900                 905                 910         


Lys Glu Lys Leu Gln Asp Asn Arg Phe Phe Lys Asn Leu Gln Arg Glu 
        915                 920                 925             


Gly Arg Leu Thr Leu Asp Lys Ile Ala Val Leu Lys Glu Gly Asp Leu 
    930                 935                 940                 


Tyr Pro Asp Lys Gly Gly Glu Lys Phe Ile Ser Leu Ser Lys Asp Arg 
945                 950                 955                 960 


Lys Cys Val Thr Thr His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln 
                965                 970                 975     


Lys Arg Phe Trp Thr Arg Thr His Gly Phe Tyr Lys Val Tyr Cys Lys 
            980                 985                 990         


Ala Tyr Gln Val Asp Gly Gln Thr  Val Tyr Ile Pro Glu  Ser Lys Asp 
        995                 1000                 1005             


Gln Lys  Gln Lys Ile Ile Glu  Glu Phe Gly Glu Gly  Tyr Phe Ile 
    1010                 1015                 1020             


Leu Lys  Asp Gly Val Tyr Glu  Trp Val Asn Ala Gly  Lys Gly Gly 
    1025                 1030                 1035             


Ser Gly  Gly Ser Ser Glu Val  Glu Phe Ser His Glu  Tyr Trp Met 
    1040                 1045                 1050             


Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu 
    1055                 1060                 1065             


Val Pro  Val Gly Ala Val Leu  Val Leu Asn Asn Arg  Val Ile Gly 
    1070                 1075                 1080             


Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His Asp Pro  Thr Ala His 
    1085                 1090                 1095             


Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn 
    1100                 1105                 1110             


Tyr Arg  Leu Tyr Asp Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys 
    1115                 1120                 1125             


Val Met  Cys Ala Gly Ala Met  Ile His Ser Arg Ile  Gly Arg Val 
    1130                 1135                 1140             


Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu 
    1145                 1150                 1155             


Met Asp  Val Leu His His Pro  Gly Met Asn His Arg  Val Glu Ile 
    1160                 1165                 1170             


Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Arg 
    1175                 1180                 1185             


Phe Phe  Arg Met Pro Arg Arg  Val Phe Asn Ala Gln  Lys Lys Ala 
    1190                 1195                 1200             


Gln Ser  Ser Thr Asp Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr 
    1205                 1210                 1215             


Ser Glu  Ser Ala Thr Pro Glu  Ser Ser Gly Leu Lys  Ile Lys Lys 
    1220                 1225                 1230             


Gly Ser  Ser Lys Gln Ser Ser  Ser Glu Leu Val Asp  Ser Asp Ile 
    1235                 1240                 1245             


Leu Lys  Asp Ser Phe Asp Leu  Ala Ser Glu Leu Lys  Gly Glu Lys 
    1250                 1255                 1260             


Leu Met  Leu Tyr Arg Asp Pro  Ser Gly Asn Val Phe  Pro Ser Asp 
    1265                 1270                 1275             


Lys Trp  Met Ala Ala Gly Val  Phe Phe Gly Lys Leu  Glu Arg Ile 
    1280                 1285                 1290             


Leu Ile  Ser Lys Leu Thr Asn  Gln Tyr Ser Ile Ser  Thr Ile Glu 
    1295                 1300                 1305             


Asp Asp  Ser Ser Lys Gln Ser  Met Lys Arg Pro Ala  Ala Thr Lys 
    1310                 1315                 1320             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys Gly Ser Tyr  Pro Tyr Asp 
    1325                 1330                 1335             


Val Pro  Asp Tyr Ala Tyr Pro  Tyr Asp Val Pro Asp  Tyr Ala Tyr 
    1340                 1345                 1350             


Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1355                 1360     


<210>  13
<211>  208
<212>  PRT
<213>  Petromyzon marinus


<220>
<221>  MISC_FEATURE
<222>  (1)..(208)
<223>  tr|A5H718|A5H718_PETMA Cytosine deaminase OS=Petromyzon marinus 
       OX=7757 PE=2 SV=1 amino acid sequence; PmCDA1 polypeptide 
       sequence

<400>  13

Met Thr Asp Ala Glu Tyr Val Arg Ile His Glu Lys Leu Asp Ile Tyr 
1               5                   10                  15      


Thr Phe Lys Lys Gln Phe Phe Asn Asn Lys Lys Ser Val Ser His Arg 
            20                  25                  30          


Cys Tyr Val Leu Phe Glu Leu Lys Arg Arg Gly Glu Arg Arg Ala Cys 
        35                  40                  45              


Phe Trp Gly Tyr Ala Val Asn Lys Pro Gln Ser Gly Thr Glu Arg Gly 
    50                  55                  60                  


Ile His Ala Glu Ile Phe Ser Ile Arg Lys Val Glu Glu Tyr Leu Arg 
65                  70                  75                  80  


Asp Asn Pro Gly Gln Phe Thr Ile Asn Trp Tyr Ser Ser Trp Ser Pro 
                85                  90                  95      


Cys Ala Asp Cys Ala Glu Lys Ile Leu Glu Trp Tyr Asn Gln Glu Leu 
            100                 105                 110         


Arg Gly Asn Gly His Thr Leu Lys Ile Trp Ala Cys Lys Leu Tyr Tyr 
        115                 120                 125             


Glu Lys Asn Ala Arg Asn Gln Ile Gly Leu Trp Asn Leu Arg Asp Asn 
    130                 135                 140                 


Gly Val Gly Leu Asn Val Met Val Ser Glu His Tyr Gln Cys Cys Arg 
145                 150                 155                 160 


Lys Ile Phe Ile Gln Ser Ser His Asn Gln Leu Asn Glu Asn Arg Trp 
                165                 170                 175     


Leu Glu Lys Thr Leu Lys Arg Ala Glu Lys Arg Arg Ser Glu Leu Ser 
            180                 185                 190         


Ile Met Ile Gln Val Lys Ile Leu His Thr Thr Lys Ser Pro Ala Val 
        195                 200                 205             


<210>  14
<211>  766
<212>  DNA
<213>  Petromyzon marinus


<220>
<221>  misc_feature
<222>  (1)..(766)
<223>  EF094822.1 Petromyzon marinus isolate PmCDA.21 cytosine deaminase
       mRNA, complete cds; PmCDA1 polynucleotide sequence

<400>  14
tgacacgaca cagccgtgta tatgaggaag ggtagctgga tggggggggg gggaatacgt       60

tcagagagga cattagcgag cgtcttgttg gtggccttga gtctagacac ctgcagacat      120

gaccgacgct gagtacgtga gaatccatga gaagttggac atctacacgt ttaagaaaca      180

gtttttcaac aacaaaaaat ccgtgtcgca tagatgctac gttctctttg aattaaaacg      240

acggggtgaa cgtagagcgt gtttttgggg ctatgctgtg aataaaccac agagcgggac      300

agaacgtgga attcacgccg aaatctttag cattagaaaa gtcgaagaat acctgcgcga      360

caaccccgga caattcacga taaattggta ctcatcctgg agtccttgtg cagattgcgc      420

tgaaaagatc ttagaatggt ataaccagga gctgcggggg aacggccaca ctttgaaaat      480

ctgggcttgc aaactctatt acgagaaaaa tgcgaggaat caaattgggc tgtggaacct      540

cagagataac ggggttgggt tgaatgtaat ggtaagtgaa cactaccaat gttgcaggaa      600

aatattcatc caatcgtcgc acaatcaatt gaatgagaat agatggcttg agaagacttt      660

gaagcgagct gaaaaacgac ggagcgagtt gtccattatg attcaggtaa aaatactcca      720

caccactaag agtcctgctg tttaagaggc tatgcggatg gttttc                     766


<210>  15
<211>  145
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(145)
<223>  tr|Q6QJ80|Q6QJ80_HUMAN Activation-induced cytidine deaminase 
       OS=Homo sapiens OX=9606 GN=AICDA PE=2 SV=1; AID polypeptide 
       sequence

<400>  15

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Ala Pro 
    130                 135                 140                 


Val 
145 


<210>  16
<211>  10681
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(10681)
<223>  NG_011588.1:5001-15681 Homo sapiens activation induced cytidine 
       deaminase (AICDA), RefSeqGene (LRG_17) on chromosome 12; nucleic 
       acid sequence of the CDS of human AID; polynucleotide sequence

<400>  16
agagaaccat cattaattga agtgagattt ttctggcctg agacttgcag ggaggcaaga       60

agacactctg gacaccacta tggacaggta aagaggcagt cttctcgtgg gtgattgcac      120

tggccttcct ctcagagcaa atctgagtaa tgagactggt agctatccct ttctctcatg      180

taactgtctg actgataaga tcagcttgat caatatgcat atatattttt tgatctgtct      240

ccttttcttc tattcagatc ttatacgctg tcagcccaat tctttctgtt tcagacttct      300

cttgatttcc ctctttttca tgtggcaaaa gaagtagtgc gtacaatgta ctgattcgtc      360

ctgagatttg taccatggtt gaaactaatt tatggtaata atattaacat agcaaatctt      420

tagagactca aatcatgaaa aggtaatagc agtactgtac taaaaacggt agtgctaatt      480

ttcgtaataa ttttgtaaat attcaacagt aaaacaactt gaagacacac tttcctaggg      540

aggcgttact gaaataattt agctatagta agaaaatttg taattttaga aatgccaagc      600

attctaaatt aattgcttga aagtcactat gattgtgtcc attataagga gacaaattca      660

ttcaagcaag ttatttaatg ttaaaggccc aattgttagg cagttaatgg cacttttact      720

attaactaat ctttccattt gttcagacgt agcttaactt acctcttagg tgtgaatttg      780

gttaaggtcc tcataatgtc tttatgtgca gtttttgata ggttattgtc atagaactta      840

ttctattcct acatttatga ttactatgga tgtatgagaa taacacctaa tccttatact      900

ttacctcaat ttaactcctt tataaagaac ttacattaca gaataaagat tttttaaaaa      960

tatatttttt tgtagagaca gggtcttagc ccagccgagg ctggtctcta agtcctggcc     1020

caagcgatcc tcctgcctgg gcctcctaaa gtgctggaat tatagacatg agccatcaca     1080

tccaatatac agaataaaga tttttaatgg aggatttaat gttcttcaga aaattttctt     1140

gaggtcagac aatgtcaaat gtctcctcag tttacactga gattttgaaa acaagtctga     1200

gctataggtc cttgtgaagg gtccattgga aatacttgtt caaagtaaaa tggaaagcaa     1260

aggtaaaatc agcagttgaa attcagagaa agacagaaaa ggagaaaaga tgaaattcaa     1320

caggacagaa gggaaatata ttatcattaa ggaggacagt atctgtagag ctcattagtg     1380

atggcaaaat gacttggtca ggattatttt taacccgctt gtttctggtt tgcacggctg     1440

gggatgcagc tagggttctg cctcagggag cacagctgtc cagagcagct gtcagcctgc     1500

aagcctgaaa cactccctcg gtaaagtcct tcctactcag gacagaaatg acgagaacag     1560

ggagctggaa acaggcccct aaccagagaa gggaagtaat ggatcaacaa agttaactag     1620

caggtcagga tcacgcaatt catttcactc tgactggtaa catgtgacag aaacagtgta     1680

ggcttattgt attttcatgt agagtaggac ccaaaaatcc acccaaagtc ctttatctat     1740

gccacatcct tcttatctat acttccagga cactttttct tccttatgat aaggctctct     1800

ctctctccac acacacacac acacacacac acacacacac acacacacac acaaacacac     1860

accccgccaa ccaaggtgca tgtaaaaaga tgtagattcc tctgcctttc tcatctacac     1920

agcccaggag ggtaagttaa tataagaggg atttattggt aagagatgat gcttaatctg     1980

tttaacactg ggcctcaaag agagaatttc ttttcttctg tacttattaa gcacctatta     2040

tgtgttgagc ttatatatac aaagggttat tatatgctaa tatagtaata gtaatggtgg     2100

ttggtactat ggtaattacc ataaaaatta ttatcctttt aaaataaagc taattattat     2160

tggatctttt ttagtattca ttttatgttt tttatgtttt tgatttttta aaagacaatc     2220

tcaccctgtt acccaggctg gagtgcagtg gtgcaatcat agctttctgc agtcttgaac     2280

tcctgggctc aagcaatcct cctgccttgg cctcccaaag tgttgggata cagtcatgag     2340

ccactgcatc tggcctagga tccatttaga ttaaaatatg cattttaaat tttaaaataa     2400

tatggctaat ttttacctta tgtaatgtgt atactggcaa taaatctagt ttgctgccta     2460

aagtttaaag tgctttccag taagcttcat gtacgtgagg ggagacattt aaagtgaaac     2520

agacagccag gtgtggtggc tcacgcctgt aatcccagca ctctgggagg ctgaggtggg     2580

tggatcgctt gagccctgga gttcaagacc agcctgagca acatggcaaa acgctgtttc     2640

tataacaaaa attagccggg catggtggca tgtgcctgtg gtcccagcta ctagggggct     2700

gaggcaggag aatcgttgga gcccaggagg tcaaggctgc actgagcagt gcttgcgcca     2760

ctgcactcca gcctgggtga caggaccaga ccttgcctca aaaaaataag aagaaaaatt     2820

aaaaataaat ggaaacaact acaaagagct gttgtcctag atgagctact tagttaggct     2880

gatattttgg tatttaactt ttaaagtcag ggtctgtcac ctgcactaca ttattaaaat     2940

atcaattctc aatgtatatc cacacaaaga ctggtacgtg aatgttcata gtacctttat     3000

tcacaaaacc ccaaagtaga gactatccaa atatccatca acaagtgaac aaataaacaa     3060

aatgtgctat atccatgcaa tggaatacca ccctgcagta caaagaagct acttggggat     3120

gaatcccaaa gtcatgacgc taaatgaaag agtcagacat gaaggaggag ataatgtatg     3180

ccatacgaaa ttctagaaaa tgaaagtaac ttatagttac agaaagcaaa tcagggcagg     3240

catagaggct cacacctgta atcccagcac tttgagaggc cacgtgggaa gattgctaga     3300

actcaggagt tcaagaccag cctgggcaac acagtgaaac tccattctcc acaaaaatgg     3360

gaaaaaaaga aagcaaatca gtggttgtcc tgtggggagg ggaaggactg caaagaggga     3420

agaagctctg gtggggtgag ggtggtgatt caggttctgt atcctgactg tggtagcagt     3480

ttggggtgtt tacatccaaa aatattcgta gaattatgca tcttaaatgg gtggagttta     3540

ctgtatgtaa attatacctc aatgtaagaa aaaataatgt gtaagaaaac tttcaattct     3600

cttgccagca aacgttattc aaattcctga gccctttact tcgcaaattc tctgcacttc     3660

tgccccgtac cattaggtga cagcactagc tccacaaatt ggataaatgc atttctggaa     3720

aagactaggg acaaaatcca ggcatcactt gtgctttcat atcaaccatg ctgtacagct     3780

tgtgttgctg tctgcagctg caatggggac tcttgatttc tttaaggaaa cttgggttac     3840

cagagtattt ccacaaatgc tattcaaatt agtgcttatg atatgcaaga cactgtgcta     3900

ggagccagaa aacaaagagg aggagaaatc agtcattatg tgggaacaac atagcaagat     3960

atttagatca ttttgactag ttaaaaaagc agcagagtac aaaatcacac atgcaatcag     4020

tataatccaa atcatgtaaa tatgtgcctg tagaaagact agaggaataa acacaagaat     4080

cttaacagtc attgtcatta gacactaagt ctaattatta ttattagaca ctatgatatt     4140

tgagatttaa aaaatcttta atattttaaa atttagagct cttctatttt tccatagtat     4200

tcaagtttga caatgatcaa gtattactct ttcttttttt tttttttttt ttttttttga     4260

gatggagttt tggtcttgtt gcccatgctg gagtggaatg gcatgaccat agctcactgc     4320

aacctccacc tcctgggttc aagcaaagct gtcgcctcag cctcccgggt agatgggatt     4380

acaggcgccc accaccacac tcggctaatg tttgtatttt tagtagagat ggggtttcac     4440

catgttggcc aggctggtct caaactcctg acctcagagg atccacctgc ctcagcctcc     4500

caaagtgctg ggattacaga tgtaggccac tgcgcccggc caagtattgc tcttatacat     4560

taaaaaacag gtgtgagcca ctgcgcccag ccaggtattg ctcttataca ttaaaaaata     4620

ggccggtgca gtggctcacg cctgtaatcc cagcactttg ggaagccaag gcgggcagaa     4680

cacccgaggt caggagtcca aggccagcct ggccaagatg gtgaaacccc gtctctatta     4740

aaaatacaaa cattacctgg gcatgatggt gggcgcctgt aatcccagct actcaggagg     4800

ctgaggcagg aggatccgcg gagcctggca gatctgcctg agcctgggag gttgaggcta     4860

cagtaagcca agatcatgcc agtatacttc agcctgggcg acaaagtgag accgtaacaa     4920

aaaaaaaaaa atttaaaaaa agaaatttag atcaagatcc aactgtaaaa agtggcctaa     4980

acaccacatt aaagagtttg gagtttattc tgcaggcaga agagaaccat cagggggtct     5040

tcagcatggg aatggcatgg tgcacctggt ttttgtgaga tcatggtggt gacagtgtgg     5100

ggaatgttat tttggaggga ctggaggcag acagaccggt taaaaggcca gcacaacaga     5160

taaggaggaa gaagatgagg gcttggaccg aagcagagaa gagcaaacag ggaaggtaca     5220

aattcaagaa atattggggg gtttgaatca acacatttag atgattaatt aaatatgagg     5280

actgaggaat aagaaatgag tcaaggatgg ttccaggctg ctaggctgct tacctgaggt     5340

ggcaaagtcg ggaggagtgg cagtttagga cagggggcag ttgaggaata ttgttttgat     5400

cattttgagt ttgaggtaca agttggacac ttaggtaaag actggagggg aaatctgaat     5460

atacaattat gggactgagg aacaagttta ttttattttt tgtttcgttt tcttgttgaa     5520

gaacaaattt aattgtaatc ccaagtcatc agcatctaga agacagtggc aggaggtgac     5580

tgtcttgtgg gtaagggttt ggggtccttg atgagtatct ctcaattggc cttaaatata     5640

agcaggaaaa ggagtttatg atggattcca ggctcagcag ggctcaggag ggctcaggca     5700

gccagcagag gaagtcagag catcttcttt ggtttagccc aagtaatgac ttccttaaaa     5760

agctgaagga aaatccagag tgaccagatt ataaactgta ctcttgcatt ttctctccct     5820

cctctcaccc acagcctctt gatgaaccgg aggaagtttc tttaccaatt caaaaatgtc     5880

cgctgggcta agggtcggcg tgagacctac ctgtgctacg tagtgaagag gcgtgacagt     5940

gctacatcct tttcactgga ctttggttat cttcgcaata aggtatcaat taaagtcggc     6000

tttgcaagca gtttaatggt caactgtgag tgcttttaga gccacctgct gatggtatta     6060

cttccatcct tttttggcat ttgtgtctct atcacattcc tcaaatcctt ttttttattt     6120

ctttttccat gtccatgcac ccatattaga catggcccaa aatatgtgat ttaattcctc     6180

cccagtaatg ctgggcaccc taataccact ccttccttca gtgccaagaa caactgctcc     6240

caaactgttt accagctttc ctcagcatct gaattgcctt tgagattaat taagctaaaa     6300

gcatttttat atgggagaat attatcagct tgtccaagca aaaattttaa atgtgaaaaa     6360

caaattgtgt cttaagcatt tttgaaaatt aaggaagaag aatttgggaa aaaattaacg     6420

gtggctcaat tctgtcttcc aaatgatttc ttttccctcc tactcacatg ggtcgtaggc     6480

cagtgaatac attcaacatg gtgatcccca gaaaactcag agaagcctcg gctgatgatt     6540

aattaaattg atctttcggc tacccgagag aattacattt ccaagagact tcttcaccaa     6600

aatccagatg ggtttacata aacttctgcc cacgggtatc tcctctctcc taacacgctg     6660

tgacgtctgg gcttggtgga atctcaggga agcatccgtg gggtggaagg tcatcgtctg     6720

gctcgttgtt tgatggttat attaccatgc aattttcttt gcctacattt gtattgaata     6780

catcccaatc tccttcctat tcggtgacat gacacattct atttcagaag gctttgattt     6840

tatcaagcac tttcatttac ttctcatggc agtgcctatt acttctctta caatacccat     6900

ctgtctgctt taccaaaatc tatttcccct tttcagatcc tcccaaatgg tcctcataaa     6960

ctgtcctgcc tccacctagt ggtccaggta tatttccaca atgttacatc aacaggcact     7020

tctagccatt ttccttctca aaaggtgcaa aaagcaactt cataaacaca aattaaatct     7080

tcggtgaggt agtgtgatgc tgcttcctcc caactcagcg cacttcgtct tcctcattcc     7140

acaaaaaccc atagccttcc ttcactctgc aggactagtg ctgccaaggg ttcagctcta     7200

cctactggtg tgctcttttg agcaagttgc ttagcctctc tgtaacacaa ggacaatagc     7260

tgcaagcatc cccaaagatc attgcaggag acaatgacta aggctaccag agccgcaata     7320

aaagtcagtg aattttagcg tggtcctctc tgtctctcca gaacggctgc cacgtggaat     7380

tgctcttcct ccgctacatc tcggactggg acctagaccc tggccgctgc taccgcgtca     7440

cctggttcac ctcctggagc ccctgctacg actgtgcccg acatgtggcc gactttctgc     7500

gagggaaccc caacctcagt ctgaggatct tcaccgcgcg cctctacttc tgtgaggacc     7560

gcaaggctga gcccgagggg ctgcggcggc tgcaccgcgc cggggtgcaa atagccatca     7620

tgaccttcaa aggtgcgaaa gggccttccg cgcaggcgca gtgcagcagc ccgcattcgg     7680

gattgcgatg cggaatgaat gagttagtgg ggaagctcga ggggaagaag tgggcgggga     7740

ttctggttca cctctggagc cgaaattaaa gattagaagc agagaaaaga gtgaatggct     7800

cagagacaag gccccgagga aatgagaaaa tggggccagg gttgcttctt tcccctcgat     7860

ttggaacctg aactgtcttc tacccccata tccccgcctt tttttccttt tttttttttt     7920

gaagattatt tttactgctg gaatactttt gtagaaaacc acgaaagaac tttcaaagcc     7980

tgggaagggc tgcatgaaaa ttcagttcgt ctctccagac agcttcggcg catccttttg     8040

gtaaggggct tcctcgcttt ttaaattttc tttctttctc tacagtcttt tttggagttt     8100

cgtatatttc ttatattttc ttattgttca atcactctca gttttcatct gatgaaaact     8160

ttatttctcc tccacatcag ctttttcttc tgctgtttca ccattcagag ccctctgcta     8220

aggttccttt tccctccctt ttctttcttt tgttgtttca catctttaaa tttctgtctc     8280

tccccagggt tgcgtttcct tcctggtcag aattcttttc tccttttttt tttttttttt     8340

tttttttttt aaacaaacaa acaaaaaacc caaaaaaact ctttcccaat ttactttctt     8400

ccaacatgtt acaaagccat ccactcagtt tagaagactc tccggcccca ccgaccccca     8460

acctcgtttt gaagccattc actcaatttg cttctctctt tctctacagc ccctgtatga     8520

ggttgatgac ttacgagacg catttcgtac tttgggactt tgatagcaac ttccaggaat     8580

gtcacacacg atgaaatatc tctgctgaag acagtggata aaaaacagtc cttcaagtct     8640

tctctgtttt tattcttcaa ctctcacttt cttagagttt acagaaaaaa tatttatata     8700

cgactcttta aaaagatcta tgtcttgaaa atagagaagg aacacaggtc tggccaggga     8760

cgtgctgcaa ttggtgcagt tttgaatgca acattgtccc ctactgggaa taacagaact     8820

gcaggacctg ggagcatcct aaagtgtcaa cgtttttcta tgacttttag gtaggatgag     8880

agcagaaggt agatcctaaa aagcatggtg agaggatcaa atgtttttat atcaacatcc     8940

tttattattt gattcatttg agttaacagt ggtgttagtg atagattttt ctattctttt     9000

cccttgacgt ttactttcaa gtaacacaaa ctcttccatc aggccatgat ctataggacc     9060

tcctaatgag agtatctggg tgattgtgac cccaaaccat ctctccaaag cattaatatc     9120

caatcatgcg ctgtatgttt taatcagcag aagcatgttt ttatgtttgt acaaaagaag     9180

attgttatgg gtggggatgg aggtatagac catgcatggt caccttcaag ctactttaat     9240

aaaggatctt aaaatgggca ggaggactgt gaacaagaca ccctaataat gggttgatgt     9300

ctgaagtagc aaatcttctg gaaacgcaaa ctcttttaag gaagtcccta atttagaaac     9360

acccacaaac ttcacatatc ataattagca aacaattgga aggaagttgc ttgaatgttg     9420

gggagaggaa aatctattgg ctctcgtggg tctcttcatc tcagaaatgc caatcaggtc     9480

aaggtttgct acattttgta tgtgtgtgat gcttctccca aaggtatatt aactatataa     9540

gagagttgtg acaaaacaga atgataaagc tgcgaaccgt ggcacacgct catagttcta     9600

gctgcttggg aggttgagga gggaggatgg cttgaacaca ggtgttcaag gccagcctgg     9660

gcaacataac aagatcctgt ctctcaaaaa aaaaaaaaaa aaaaagaaag agagagggcc     9720

gggcgtggtg gctcacgcct gtaatcccag cactttggga ggccgagccg ggcggatcac     9780

ctgtggtcag gagtttgaga ccagcctggc caacatggca aaaccccgtc tgtactcaaa     9840

atgcaaaaat tagccaggcg tggtagcagg cacctgtaat cccagctact tgggaggctg     9900

aggcaggaga atcgcttgaa cccaggaggt ggaggttgca gtaagctgag atcgtgccgt     9960

tgcactccag cctgggcgac aagagcaaga ctctgtctca gaaaaaaaaa aaaaaaagag    10020

agagagagag aaagagaaca atatttggga gagaaggatg gggaagcatt gcaaggaaat    10080

tgtgctttat ccaacaaaat gtaaggagcc aataagggat ccctatttgt ctcttttggt    10140

gtctatttgt ccctaacaac tgtctttgac agtgagaaaa atattcagaa taaccatatc    10200

cctgtgccgt tattacctag caacccttgc aatgaagatg agcagatcca caggaaaact    10260

tgaatgcaca actgtcttat tttaatctta ttgtacataa gtttgtaaaa gagttaaaaa    10320

ttgttacttc atgtattcat ttatatttta tattattttg cgtctaatga ttttttatta    10380

acatgatttc cttttctgat atattgaaat ggagtctcaa agcttcataa atttataact    10440

ttagaaatga ttctaataac aacgtatgta attgtaacat tgcagtaatg gtgctacgaa    10500

gccatttctc ttgattttta gtaaactttt atgacagcaa atttgcttct ggctcacttt    10560

caatcagtta aataaatgat aaataatttt ggaagctgtg aagataaaat accaaataaa    10620

ataatataaa agtgatttat atgaagttaa aataaaaaat cagtatgatg gaataaactt    10680

g                                                                    10681


<210>  17
<211>  198
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(198)
<223>  human AID polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  Nuclear localization sequence

<220>
<221>  MISC_FEATURE
<222>  (183)..(198)
<223>  Nuclear export signal

<400>  17

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  18
<211>  198
<212>  PRT
<213>  Canis lupus familiaris


<220>
<221>  MISC_FEATURE
<222>  (1)..(198)
<223>  Canine AID (clAID) polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  Nuclear localization sequence

<220>
<221>  MISC_FEATURE
<222>  (183)..(198)
<223>  Nuclear export signal

<400>  18

Met Asp Ser Leu Leu Met Lys Gln Arg Lys Phe Leu Tyr His Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg His Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly His 
        35                  40                  45              


Leu Arg Asn Lys Ser Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Tyr Pro Asn Leu Ser Leu Arg Ile Phe Ala Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn Arg Glu Lys Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  19
<211>  199
<212>  PRT
<213>  Bos Taurus


<220>
<221>  MISC_FEATURE
<222>  (1)..(199)
<223>  Bovine AID (btAID) polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  Nuclear localization sequence

<220>
<221>  MISC_FEATURE
<222>  (183)..(199)
<223>  Nuclear export signal

<400>  19

Met Asp Ser Leu Leu Lys Lys Gln Arg Gln Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg His Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Pro Thr Ser Phe Ser Leu Asp Phe Gly His 
        35                  40                  45              


Leu Arg Asn Lys Ala Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Tyr Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Asp Lys Glu Arg Lys Ala Glu Pro Glu Gly Leu Arg 
        115                 120                 125             


Arg Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp 
    130                 135                 140                 


Tyr Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe 
145                 150                 155                 160 


Lys Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln 
                165                 170                 175     


Leu Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp 
            180                 185                 190         


Ala Phe Arg Thr Leu Gly Leu 
        195                 


<210>  20
<211>  239
<212>  PRT
<213>  Rattus


<220>
<221>  MISC_FEATURE
<222>  (1)..(239)
<223>  Rat AID polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  Nuclear localization sequence

<220>
<221>  MISC_FEATURE
<222>  (194)..(239)
<223>  Nuclear export signal

<400>  20

Met Ala Val Gly Ser Lys Pro Lys Ala Ala Leu Val Gly Pro His Trp 
1               5                   10                  15      


Glu Arg Glu Arg Ile Trp Cys Phe Leu Cys Ser Thr Gly Leu Gly Thr 
            20                  25                  30          


Gln Gln Thr Gly Gln Thr Ser Arg Trp Leu Arg Pro Ala Ala Thr Gln 
        35                  40                  45              


Asp Pro Val Ser Pro Pro Arg Ser Leu Leu Met Lys Gln Arg Lys Phe 
    50                  55                  60                  


Leu Tyr His Phe Lys Asn Val Arg Trp Ala Lys Gly Arg His Glu Thr 
65                  70                  75                  80  


Tyr Leu Cys Tyr Val Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser 
                85                  90                  95      


Leu Asp Phe Gly Tyr Leu Arg Asn Lys Ser Gly Cys His Val Glu Leu 
            100                 105                 110         


Leu Phe Leu Arg Tyr Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys 
        115                 120                 125             


Tyr Arg Val Thr Trp Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala 
    130                 135                 140                 


Arg His Val Ala Asp Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg 
145                 150                 155                 160 


Ile Phe Thr Ala Arg Leu Thr Gly Trp Gly Ala Leu Pro Ala Gly Leu 
                165                 170                 175     


Met Ser Pro Ala Arg Pro Ser Asp Tyr Phe Tyr Cys Trp Asn Thr Phe 
            180                 185                 190         


Val Glu Asn His Glu Arg Thr Phe Lys Ala Trp Glu Gly Leu His Glu 
        195                 200                 205             


Asn Ser Val Arg Leu Ser Arg Arg Leu Arg Arg Ile Leu Leu Pro Leu 
    210                 215                 220                 


Tyr Glu Val Asp Asp Leu Arg Asp Ala Phe Arg Thr Leu Gly Leu 
225                 230                 235                 


<210>  21
<211>  198
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(198)
<223>  Mouse (mAID) AID polypeptide sequence

<400>  21

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  22
<211>  229
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(229)
<223>  rAPOBEC-1 polypeptide sequence

<400>  22

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys 
225                 


<210>  23
<211>  229
<212>  PRT
<213>  Mesocricetus auratus


<220>
<221>  MISC_FEATURE
<222>  (1)..(229)
<223>  maAPOBEC-1 polypeptide sequence

<400>  23

Met Ser Ser Glu Thr Gly Pro Val Val Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Asp Ala Phe Phe Asp Gln Gly Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Arg Trp Gly Gly Arg His 
        35                  40                  45              


Asn Ile Trp Arg His Thr Gly Gln Asn Thr Ser Arg His Val Glu Ile 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Ser Glu Arg Tyr Phe Tyr Pro Ser Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Val Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Lys Ala Ile Thr Glu Phe Leu Ser Gly His Pro Asn Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ala Ala Arg Leu Tyr His His Thr Asp Gln Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Arg Gly Val Thr Ile Arg Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Tyr Cys Tyr Cys Trp Arg Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Ser Asn Glu Val Tyr Trp Pro Arg Tyr Pro Asn Leu Trp Met Arg 
                165                 170                 175     


Leu Tyr Ala Leu Glu Leu Tyr Cys Ile His Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Lys Ile Lys Arg Arg His Gln Tyr Pro Leu Thr Phe Phe Arg Leu 
        195                 200                 205             


Asn Leu Gln Ser Cys His Tyr Gln Arg Ile Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Phe Ile 
225                 


<210>  24
<211>  236
<212>  PRT
<213>  Pongo pygmaeus


<220>
<221>  MISC_FEATURE
<222>  (1)..(236)
<223>  ppAPOBEC-1 polypeptide sequence

<400>  24

Met Thr Ser Glu Lys Gly Pro Ser Thr Gly Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Ser Trp Glu Phe Asp Val Phe Tyr Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Lys Trp Gly Met Ser Arg 
        35                  40                  45              


Lys Ile Trp Arg Ser Ser Gly Lys Asn Thr Thr Asn His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Lys Lys Phe Thr Ser Glu Arg Arg Phe His Ser Ser Ile 
65                  70                  75                  80  


Ser Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu Cys 
                85                  90                  95      


Ser Gln Ala Ile Arg Glu Phe Leu Ser Gln His Pro Gly Val Thr Leu 
            100                 105                 110         


Val Ile Tyr Val Ala Arg Leu Phe Trp His Met Asp Gln Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Val Asn Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Arg Ala Ser Glu Tyr Tyr His Cys Trp Arg Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Gly Asp Glu Ala His Trp Pro Gln Tyr Pro Pro Leu Trp Met Met 
                165                 170                 175     


Leu Tyr Ala Leu Glu Leu His Cys Ile Ile Leu Ser Leu Pro Pro Cys 
            180                 185                 190         


Leu Lys Ile Ser Arg Arg Trp Gln Asn His Leu Ala Phe Phe Arg Leu 
        195                 200                 205             


His Leu Gln Asn Cys His Tyr Gln Thr Ile Pro Pro His Ile Leu Leu 
    210                 215                 220                 


Ala Thr Gly Leu Ile His Pro Ser Val Thr Trp Arg 
225                 230                 235     


<210>  25
<211>  236
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(236)
<223>  ocAPOBEC1 polypeptide sequence

<400>  25

Met Ala Ser Glu Lys Gly Pro Ser Asn Lys Asp Tyr Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro Trp Glu Phe Glu Val Phe Phe Asp Pro Gln Glu Leu 
            20                  25                  30          


Arg Lys Glu Ala Cys Leu Leu Tyr Glu Ile Lys Trp Gly Ala Ser Ser 
        35                  40                  45              


Lys Thr Trp Arg Ser Ser Gly Lys Asn Thr Thr Asn His Val Glu Val 
    50                  55                  60                  


Asn Phe Leu Glu Lys Leu Thr Ser Glu Gly Arg Leu Gly Pro Ser Thr 
65                  70                  75                  80  


Cys Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu Cys 
                85                  90                  95      


Ser Met Ala Ile Arg Glu Phe Leu Ser Gln His Pro Gly Val Thr Leu 
            100                 105                 110         


Ile Ile Phe Val Ala Arg Leu Phe Gln His Met Asp Arg Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Lys Asp Leu Val Thr Ser Gly Val Thr Val Arg Val Met 
    130                 135                 140                 


Ser Val Ser Glu Tyr Cys Tyr Cys Trp Glu Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Gly Lys Ala Ala Gln Trp Pro Arg Tyr Pro Pro Arg Trp Met Leu 
                165                 170                 175     


Met Tyr Ala Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Lys Ile Ser Arg Arg His Gln Lys Gln Leu Thr Phe Phe Ser Leu 
        195                 200                 205             


Thr Pro Gln Tyr Cys His Tyr Lys Met Ile Pro Pro Tyr Ile Leu Leu 
    210                 215                 220                 


Ala Thr Gly Leu Leu Gln Pro Ser Val Pro Trp Arg 
225                 230                 235     


<210>  26
<211>  235
<212>  PRT
<213>  Monodelphis domestica


<220>
<221>  MISC_FEATURE
<222>  (1)..(235)
<223>  mdAPOBEC-1 polypeptide sequence

<400>  26

Met Asn Ser Lys Thr Gly Pro Ser Val Gly Asp Ala Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Lys Pro Trp Glu Phe Val Ala Phe Phe Asn Pro Gln Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Lys Trp Gly Asn Gln Asn 
        35                  40                  45              


Ile Trp Arg His Ser Asn Gln Asn Thr Ser Gln His Ala Glu Ile Asn 
    50                  55                  60                  


Phe Met Glu Lys Phe Thr Ala Glu Arg His Phe Asn Ser Ser Val Arg 
65                  70                  75                  80  


Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu Cys Ser 
                85                  90                  95      


Lys Ala Ile Arg Lys Phe Leu Asp His Tyr Pro Asn Val Thr Leu Ala 
            100                 105                 110         


Ile Phe Ile Ser Arg Leu Tyr Trp His Met Asp Gln Gln His Arg Gln 
        115                 120                 125             


Gly Leu Lys Glu Leu Val His Ser Gly Val Thr Ile Gln Ile Met Ser 
    130                 135                 140                 


Tyr Ser Glu Tyr His Tyr Cys Trp Arg Asn Phe Val Asp Tyr Pro Gln 
145                 150                 155                 160 


Gly Glu Glu Asp Tyr Trp Pro Lys Tyr Pro Tyr Leu Trp Ile Met Leu 
                165                 170                 175     


Tyr Val Leu Glu Leu His Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu 
            180                 185                 190         


Lys Ile Ser Gly Ser His Ser Asn Gln Leu Ala Leu Phe Ser Leu Asp 
        195                 200                 205             


Leu Gln Asp Cys His Tyr Gln Lys Ile Pro Tyr Asn Val Leu Val Ala 
    210                 215                 220                 


Thr Gly Leu Val Gln Pro Phe Val Thr Trp Arg 
225                 230                 235 


<210>  27
<211>  224
<212>  PRT
<213>  Pongo pygmaeus


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  ppAPOBEC-2 polypeptide sequence

<400>  27

Met Ala Gln Lys Glu Glu Ala Ala Ala Ala Thr Glu Ala Ala Ser Gln 
1               5                   10                  15      


Asn Gly Glu Asp Leu Glu Asn Leu Asp Asp Pro Glu Lys Leu Lys Glu 
            20                  25                  30          


Leu Ile Glu Leu Pro Pro Phe Glu Ile Val Thr Gly Glu Arg Leu Pro 
        35                  40                  45              


Ala Asn Phe Phe Lys Phe Gln Phe Arg Asn Val Glu Tyr Ser Ser Gly 
    50                  55                  60                  


Arg Asn Lys Thr Phe Leu Cys Tyr Val Val Glu Ala Gln Gly Lys Gly 
65                  70                  75                  80  


Gly Gln Val Gln Ala Ser Arg Gly Tyr Leu Glu Asp Glu His Ala Ala 
                85                  90                  95      


Ala His Ala Glu Glu Ala Phe Phe Asn Thr Ile Leu Pro Ala Phe Asp 
            100                 105                 110         


Pro Ala Leu Arg Tyr Asn Val Thr Trp Tyr Val Ser Ser Ser Pro Cys 
        115                 120                 125             


Ala Ala Cys Ala Asp Arg Ile Ile Lys Thr Leu Ser Lys Thr Lys Asn 
    130                 135                 140                 


Leu Arg Leu Leu Ile Leu Val Gly Arg Leu Phe Met Trp Glu Glu Leu 
145                 150                 155                 160 


Glu Ile Gln Asp Ala Leu Lys Lys Leu Lys Glu Ala Gly Cys Lys Leu 
                165                 170                 175     


Arg Ile Met Lys Pro Gln Asp Phe Glu Tyr Val Trp Gln Asn Phe Val 
            180                 185                 190         


Glu Gln Glu Glu Gly Glu Ser Lys Ala Phe Gln Pro Trp Glu Asp Ile 
        195                 200                 205             


Gln Glu Asn Phe Leu Tyr Tyr Glu Glu Lys Leu Ala Asp Ile Leu Lys 
    210                 215                 220                 


<210>  28
<211>  224
<212>  PRT
<213>  Bos Taurus


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  btAPOBEC-2 polypeptide sequence

<400>  28

Met Ala Gln Lys Glu Glu Ala Ala Ala Ala Ala Glu Pro Ala Ser Gln 
1               5                   10                  15      


Asn Gly Glu Glu Val Glu Asn Leu Glu Asp Pro Glu Lys Leu Lys Glu 
            20                  25                  30          


Leu Ile Glu Leu Pro Pro Phe Glu Ile Val Thr Gly Glu Arg Leu Pro 
        35                  40                  45              


Ala His Tyr Phe Lys Phe Gln Phe Arg Asn Val Glu Tyr Ser Ser Gly 
    50                  55                  60                  


Arg Asn Lys Thr Phe Leu Cys Tyr Val Val Glu Ala Gln Ser Lys Gly 
65                  70                  75                  80  


Gly Gln Val Gln Ala Ser Arg Gly Tyr Leu Glu Asp Glu His Ala Thr 
                85                  90                  95      


Asn His Ala Glu Glu Ala Phe Phe Asn Ser Ile Met Pro Thr Phe Asp 
            100                 105                 110         


Pro Ala Leu Arg Tyr Met Val Thr Trp Tyr Val Ser Ser Ser Pro Cys 
        115                 120                 125             


Ala Ala Cys Ala Asp Arg Ile Val Lys Thr Leu Asn Lys Thr Lys Asn 
    130                 135                 140                 


Leu Arg Leu Leu Ile Leu Val Gly Arg Leu Phe Met Trp Glu Glu Pro 
145                 150                 155                 160 


Glu Ile Gln Ala Ala Leu Arg Lys Leu Lys Glu Ala Gly Cys Arg Leu 
                165                 170                 175     


Arg Ile Met Lys Pro Gln Asp Phe Glu Tyr Ile Trp Gln Asn Phe Val 
            180                 185                 190         


Glu Gln Glu Glu Gly Glu Ser Lys Ala Phe Glu Pro Trp Glu Asp Ile 
        195                 200                 205             


Gln Glu Asn Phe Leu Tyr Tyr Glu Glu Lys Leu Ala Asp Ile Leu Lys 
    210                 215                 220                 


<210>  29
<211>  440
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(440)
<223>  mAPOBEC-3-(1) polypeptide sequence

<400>  29

Met Gln Pro Gln Arg Leu Gly Pro Arg Ala Gly Met Gly Pro Phe Cys 
1               5                   10                  15      


Leu Gly Cys Ser His Arg Lys Cys Tyr Ser Pro Ile Arg Asn Leu Ile 
            20                  25                  30          


Ser Gln Glu Thr Phe Lys Phe His Phe Lys Asn Leu Gly Tyr Ala Lys 
        35                  40                  45              


Gly Arg Lys Asp Thr Phe Leu Cys Tyr Glu Val Thr Arg Lys Asp Cys 
    50                  55                  60                  


Asp Ser Pro Val Ser Leu His His Gly Val Phe Lys Asn Lys Asp Asn 
65                  70                  75                  80  


Ile His Ala Glu Ile Cys Phe Leu Tyr Trp Phe His Asp Lys Val Leu 
                85                  90                  95      


Lys Val Leu Ser Pro Arg Glu Glu Phe Lys Ile Thr Trp Tyr Met Ser 
            100                 105                 110         


Trp Ser Pro Cys Phe Glu Cys Ala Glu Gln Ile Val Arg Phe Leu Ala 
        115                 120                 125             


Thr His His Asn Leu Ser Leu Asp Ile Phe Ser Ser Arg Leu Tyr Asn 
    130                 135                 140                 


Val Gln Asp Pro Glu Thr Gln Gln Asn Leu Cys Arg Leu Val Gln Glu 
145                 150                 155                 160 


Gly Ala Gln Val Ala Ala Met Asp Leu Tyr Glu Phe Lys Lys Cys Trp 
                165                 170                 175     


Lys Lys Phe Val Asp Asn Gly Gly Arg Arg Phe Arg Pro Trp Lys Arg 
            180                 185                 190         


Leu Leu Thr Asn Phe Arg Tyr Gln Asp Ser Lys Leu Gln Glu Ile Leu 
        195                 200                 205             


Arg Pro Cys Tyr Ile Ser Val Pro Ser Ser Ser Ser Ser Thr Leu Ser 
    210                 215                 220                 


Asn Ile Cys Leu Thr Lys Gly Leu Pro Glu Thr Arg Phe Trp Val Glu 
225                 230                 235                 240 


Gly Arg Arg Met Asp Pro Leu Ser Glu Glu Glu Phe Tyr Ser Gln Phe 
                245                 250                 255     


Tyr Asn Gln Arg Val Lys His Leu Cys Tyr Tyr His Arg Met Lys Pro 
            260                 265                 270         


Tyr Leu Cys Tyr Gln Leu Glu Gln Phe Asn Gly Gln Ala Pro Leu Lys 
        275                 280                 285             


Gly Cys Leu Leu Ser Glu Lys Gly Lys Gln His Ala Glu Ile Leu Phe 
    290                 295                 300                 


Leu Asp Lys Ile Arg Ser Met Glu Leu Ser Gln Val Thr Ile Thr Cys 
305                 310                 315                 320 


Tyr Leu Thr Trp Ser Pro Cys Pro Asn Cys Ala Trp Gln Leu Ala Ala 
                325                 330                 335     


Phe Lys Arg Asp Arg Pro Asp Leu Ile Leu His Ile Tyr Thr Ser Arg 
            340                 345                 350         


Leu Tyr Phe His Trp Lys Arg Pro Phe Gln Lys Gly Leu Cys Ser Leu 
        355                 360                 365             


Trp Gln Ser Gly Ile Leu Val Asp Val Met Asp Leu Pro Gln Phe Thr 
    370                 375                 380                 


Asp Cys Trp Thr Asn Phe Val Asn Pro Lys Arg Pro Phe Trp Pro Trp 
385                 390                 395                 400 


Lys Gly Leu Glu Ile Ile Ser Arg Arg Thr Gln Arg Arg Leu Arg Arg 
                405                 410                 415     


Ile Lys Glu Ser Trp Gly Leu Gln Asp Leu Val Asn Asp Phe Gly Asn 
            420                 425                 430         


Leu Gln Leu Gly Pro Pro Met Ser 
        435                 440 


<210>  30
<211>  429
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(429)
<223>  APOBEC-3-(2) (Mouse APOBEC-3) polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (71)..(108)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (288)..(319)
<223>  Nucleic acid editing domain

<400>  30

Met Gly Pro Phe Cys Leu Gly Cys Ser His Arg Lys Cys Tyr Ser Pro 
1               5                   10                  15      


Ile Arg Asn Leu Ile Ser Gln Glu Thr Phe Lys Phe His Phe Lys Asn 
            20                  25                  30          


Leu Gly Tyr Ala Lys Gly Arg Lys Asp Thr Phe Leu Cys Tyr Glu Val 
        35                  40                  45              


Thr Arg Lys Asp Cys Asp Ser Pro Val Ser Leu His His Gly Val Phe 
    50                  55                  60                  


Lys Asn Lys Asp Asn Ile His Ala Glu Ile Cys Phe Leu Tyr Trp Phe 
65                  70                  75                  80  


His Asp Lys Val Leu Lys Val Leu Ser Pro Arg Glu Glu Phe Lys Ile 
                85                  90                  95      


Thr Trp Tyr Met Ser Trp Ser Pro Cys Phe Glu Cys Ala Glu Gln Ile 
            100                 105                 110         


Val Arg Phe Leu Ala Thr His His Asn Leu Ser Leu Asp Ile Phe Ser 
        115                 120                 125             


Ser Arg Leu Tyr Asn Val Gln Asp Pro Glu Thr Gln Gln Asn Leu Cys 
    130                 135                 140                 


Arg Leu Val Gln Glu Gly Ala Gln Val Ala Ala Met Asp Leu Tyr Glu 
145                 150                 155                 160 


Phe Lys Lys Cys Trp Lys Lys Phe Val Asp Asn Gly Gly Arg Arg Phe 
                165                 170                 175     


Arg Pro Trp Lys Arg Leu Leu Thr Asn Phe Arg Tyr Gln Asp Ser Lys 
            180                 185                 190         


Leu Gln Glu Ile Leu Arg Pro Cys Tyr Ile Pro Val Pro Ser Ser Ser 
        195                 200                 205             


Ser Ser Thr Leu Ser Asn Ile Cys Leu Thr Lys Gly Leu Pro Glu Thr 
    210                 215                 220                 


Arg Phe Cys Val Glu Gly Arg Arg Met Asp Pro Leu Ser Glu Glu Glu 
225                 230                 235                 240 


Phe Tyr Ser Gln Phe Tyr Asn Gln Arg Val Lys His Leu Cys Tyr Tyr 
                245                 250                 255     


His Arg Met Lys Pro Tyr Leu Cys Tyr Gln Leu Glu Gln Phe Asn Gly 
            260                 265                 270         


Gln Ala Pro Leu Lys Gly Cys Leu Leu Ser Glu Lys Gly Lys Gln His 
        275                 280                 285             


Ala Glu Ile Leu Phe Leu Asp Lys Ile Arg Ser Met Glu Leu Ser Gln 
    290                 295                 300                 


Val Thr Ile Thr Cys Tyr Leu Thr Trp Ser Pro Cys Pro Asn Cys Ala 
305                 310                 315                 320 


Trp Gln Leu Ala Ala Phe Lys Arg Asp Arg Pro Asp Leu Ile Leu His 
                325                 330                 335     


Ile Tyr Thr Ser Arg Leu Tyr Phe His Trp Lys Arg Pro Phe Gln Lys 
            340                 345                 350         


Gly Leu Cys Ser Leu Trp Gln Ser Gly Ile Leu Val Asp Val Met Asp 
        355                 360                 365             


Leu Pro Gln Phe Thr Asp Cys Trp Thr Asn Phe Val Asn Pro Lys Arg 
    370                 375                 380                 


Pro Phe Trp Pro Trp Lys Gly Leu Glu Ile Ile Ser Arg Arg Thr Gln 
385                 390                 395                 400 


Arg Arg Leu Arg Arg Ile Lys Glu Ser Trp Gly Leu Gln Asp Leu Val 
                405                 410                 415     


Asn Asp Phe Gly Asn Leu Gln Leu Gly Pro Pro Met Ser 
            420                 425                 


<210>  31
<211>  430
<212>  PRT
<213>  Rattus


<220>
<221>  MISC_FEATURE
<222>  (1)..(430)
<223>  APOBEC-3 polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (72)..(109)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (289)..(320)
<223>  Nucleic acid editing domain

<400>  31

Met Gly Pro Phe Cys Leu Gly Cys Ser His Arg Lys Cys Tyr Ser Pro 
1               5                   10                  15      


Ile Arg Asn Leu Ile Ser Gln Glu Thr Phe Lys Phe His Phe Lys Asn 
            20                  25                  30          


Arg Leu Arg Tyr Ala Ile Asp Arg Lys Asp Thr Phe Leu Cys Tyr Glu 
        35                  40                  45              


Val Thr Arg Lys Asp Cys Asp Ser Pro Val Ser Leu His His Gly Val 
    50                  55                  60                  


Phe Lys Asn Lys Asp Asn Ile His Ala Glu Ile Cys Phe Leu Tyr Trp 
65                  70                  75                  80  


Phe His Asp Lys Val Leu Lys Val Leu Ser Pro Arg Glu Glu Phe Lys 
                85                  90                  95      


Ile Thr Trp Tyr Met Ser Trp Ser Pro Cys Phe Glu Cys Ala Glu Gln 
            100                 105                 110         


Val Leu Arg Phe Leu Ala Thr His His Asn Leu Ser Leu Asp Ile Phe 
        115                 120                 125             


Ser Ser Arg Leu Tyr Asn Ile Arg Asp Pro Glu Asn Gln Gln Asn Leu 
    130                 135                 140                 


Cys Arg Leu Val Gln Glu Gly Ala Gln Val Ala Ala Met Asp Leu Tyr 
145                 150                 155                 160 


Glu Phe Lys Lys Cys Trp Lys Lys Phe Val Asp Asn Gly Gly Arg Arg 
                165                 170                 175     


Phe Arg Pro Trp Lys Lys Leu Leu Thr Asn Phe Arg Tyr Gln Asp Ser 
            180                 185                 190         


Lys Leu Gln Glu Ile Leu Arg Pro Cys Tyr Ile Pro Val Pro Ser Ser 
        195                 200                 205             


Ser Ser Ser Thr Leu Ser Asn Ile Cys Leu Thr Lys Gly Leu Pro Glu 
    210                 215                 220                 


Thr Arg Phe Cys Val Glu Arg Arg Arg Val His Leu Leu Ser Glu Glu 
225                 230                 235                 240 


Glu Phe Tyr Ser Gln Phe Tyr Asn Gln Arg Val Lys His Leu Cys Tyr 
                245                 250                 255     


Tyr His Gly Val Lys Pro Tyr Leu Cys Tyr Gln Leu Glu Gln Phe Asn 
            260                 265                 270         


Gly Gln Ala Pro Leu Lys Gly Cys Leu Leu Ser Glu Lys Gly Lys Gln 
        275                 280                 285             


His Ala Glu Ile Leu Phe Leu Asp Lys Ile Arg Ser Met Glu Leu Ser 
    290                 295                 300                 


Gln Val Ile Ile Thr Cys Tyr Leu Thr Trp Ser Pro Cys Pro Asn Cys 
305                 310                 315                 320 


Ala Trp Gln Leu Ala Ala Phe Lys Arg Asp Arg Pro Asp Leu Ile Leu 
                325                 330                 335     


His Ile Tyr Thr Ser Arg Leu Tyr Phe His Trp Lys Arg Pro Phe Gln 
            340                 345                 350         


Lys Gly Leu Cys Ser Leu Trp Gln Ser Gly Ile Leu Val Asp Val Met 
        355                 360                 365             


Asp Leu Pro Gln Phe Thr Asp Cys Trp Thr Asn Phe Val Asn Pro Lys 
    370                 375                 380                 


Arg Pro Phe Trp Pro Trp Lys Gly Leu Glu Ile Ile Ser Arg Arg Thr 
385                 390                 395                 400 


Gln Arg Arg Leu His Arg Ile Lys Glu Ser Trp Gly Leu Gln Asp Leu 
                405                 410                 415     


Val Asn Asp Phe Gly Asn Leu Gln Leu Gly Pro Pro Met Ser 
            420                 425                 430 


<210>  32
<211>  199
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(199)
<223>  hAPOBEC-3A polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (70)..(106)
<223>  Nucleic acid editing domain

<400>  32

Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His 
1               5                   10                  15      


Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr 
            20                  25                  30          


Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met 
        35                  40                  45              


Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys 
    50                  55                  60                  


Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro 
65                  70                  75                  80  


Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile 
                85                  90                  95      


Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala 
            100                 105                 110         


Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg 
        115                 120                 125             


Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg 
    130                 135                 140                 


Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His 
145                 150                 155                 160 


Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp 
                165                 170                 175     


Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala 
            180                 185                 190         


Ile Leu Gln Asn Gln Gly Asn 
        195                 


<210>  33
<211>  373
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(373)
<223>  hAPOBEC-3F polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (65)..(99)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (248)..(283)
<223>  Nucleic acid editing domain

<400>  33

Met Lys Pro His Phe Arg Asn Thr Val Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Ser Tyr Asn Phe Tyr Asn Arg Pro Ile Leu Ser Arg Arg Asn Thr 
            20                  25                  30          


Val Trp Leu Cys Tyr Glu Val Lys Thr Lys Gly Pro Ser Arg Pro Arg 
        35                  40                  45              


Leu Asp Ala Lys Ile Phe Arg Gly Gln Val Tyr Ser Gln Pro Glu His 
    50                  55                  60                  


His Ala Glu Met Cys Phe Leu Ser Trp Phe Cys Gly Asn Gln Leu Pro 
65                  70                  75                  80  


Ala Tyr Lys Cys Phe Gln Ile Thr Trp Phe Val Ser Trp Thr Pro Cys 
                85                  90                  95      


Pro Asp Cys Val Ala Lys Leu Ala Glu Phe Leu Ala Glu His Pro Asn 
            100                 105                 110         


Val Thr Leu Thr Ile Ser Ala Ala Arg Leu Tyr Tyr Tyr Trp Glu Arg 
        115                 120                 125             


Asp Tyr Arg Arg Ala Leu Cys Arg Leu Ser Gln Ala Gly Ala Arg Val 
    130                 135                 140                 


Lys Ile Met Asp Asp Glu Glu Phe Ala Tyr Cys Trp Glu Asn Phe Val 
145                 150                 155                 160 


Tyr Ser Glu Gly Gln Pro Phe Met Pro Trp Tyr Lys Phe Asp Asp Asn 
                165                 170                 175     


Tyr Ala Phe Leu His Arg Thr Leu Lys Glu Ile Leu Arg Asn Pro Met 
            180                 185                 190         


Glu Ala Met Tyr Pro His Ile Phe Tyr Phe His Phe Lys Asn Leu Arg 
        195                 200                 205             


Lys Ala Tyr Gly Arg Asn Glu Ser Trp Leu Cys Phe Thr Met Glu Val 
    210                 215                 220                 


Val Lys His His Ser Pro Val Ser Trp Lys Arg Gly Val Phe Arg Asn 
225                 230                 235                 240 


Gln Val Asp Pro Glu Thr His Cys His Ala Glu Arg Cys Phe Leu Ser 
                245                 250                 255     


Trp Phe Cys Asp Asp Ile Leu Ser Pro Asn Thr Asn Tyr Glu Val Thr 
            260                 265                 270         


Trp Tyr Thr Ser Trp Ser Pro Cys Pro Glu Cys Ala Gly Glu Val Ala 
        275                 280                 285             


Glu Phe Leu Ala Arg His Ser Asn Val Asn Leu Thr Ile Phe Thr Ala 
    290                 295                 300                 


Arg Leu Tyr Tyr Phe Trp Asp Thr Asp Tyr Gln Glu Gly Leu Arg Ser 
305                 310                 315                 320 


Leu Ser Gln Glu Gly Ala Ser Val Glu Ile Met Gly Tyr Lys Asp Phe 
                325                 330                 335     


Lys Tyr Cys Trp Glu Asn Phe Val Tyr Asn Asp Asp Glu Pro Phe Lys 
            340                 345                 350         


Pro Trp Lys Gly Leu Lys Tyr Asn Phe Leu Phe Leu Asp Ser Lys Leu 
        355                 360                 365             


Gln Glu Ile Leu Glu 
    370             


<210>  34
<211>  370
<212>  PRT
<213>  Rhesus macaque


<220>
<221>  MISC_FEATURE
<222>  (1)..(370)
<223>  Rhesus macaque APOBEC-3G polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(61)
<223>  Cytoplasmic localization signal

<400>  34

Met Val Glu Pro Met Asp Pro Arg Thr Phe Val Ser Asn Phe Asn Asn 
1               5                   10                  15      


Arg Pro Ile Leu Ser Gly Leu Asn Thr Val Trp Leu Cys Cys Glu Val 
            20                  25                  30          


Lys Thr Lys Asp Pro Ser Gly Pro Pro Leu Asp Ala Lys Ile Phe Gln 
        35                  40                  45              


Gly Lys Val Tyr Ser Lys Ala Lys Tyr His Pro Glu Met Arg Phe Leu 
    50                  55                  60                  


Arg Trp Phe His Lys Trp Arg Gln Leu His His Asp Gln Glu Tyr Lys 
65                  70                  75                  80  


Val Thr Trp Tyr Val Ser Trp Ser Pro Cys Thr Arg Cys Ala Asn Ser 
                85                  90                  95      


Val Ala Thr Phe Leu Ala Lys Asp Pro Lys Val Thr Leu Thr Ile Phe 
            100                 105                 110         


Val Ala Arg Leu Tyr Tyr Phe Trp Lys Pro Asp Tyr Gln Gln Ala Leu 
        115                 120                 125             


Arg Ile Leu Cys Gln Lys Arg Gly Gly Pro His Ala Thr Met Lys Ile 
    130                 135                 140                 


Met Asn Tyr Asn Glu Phe Gln Asp Cys Trp Asn Lys Phe Val Asp Gly 
145                 150                 155                 160 


Arg Gly Lys Pro Phe Lys Pro Arg Asn Asn Leu Pro Lys His Tyr Thr 
                165                 170                 175     


Leu Leu Gln Ala Thr Leu Gly Glu Leu Leu Arg His Leu Met Asp Pro 
            180                 185                 190         


Gly Thr Phe Thr Ser Asn Phe Asn Asn Lys Pro Trp Val Ser Gly Gln 
        195                 200                 205             


His Glu Thr Tyr Leu Cys Tyr Lys Val Glu Arg Leu His Asn Asp Thr 
    210                 215                 220                 


Trp Val Pro Leu Asn Gln His Arg Gly Phe Leu Arg Asn Gln Ala Pro 
225                 230                 235                 240 


Asn Ile His Gly Phe Pro Lys Gly Arg His Ala Glu Leu Cys Phe Leu 
                245                 250                 255     


Asp Leu Ile Pro Phe Trp Lys Leu Asp Gly Gln Gln Tyr Arg Val Thr 
            260                 265                 270         


Cys Phe Thr Ser Trp Ser Pro Cys Phe Ser Cys Ala Gln Glu Met Ala 
        275                 280                 285             


Lys Phe Ile Ser Asn Asn Glu His Val Ser Leu Cys Ile Phe Ala Ala 
    290                 295                 300                 


Arg Ile Tyr Asp Asp Gln Gly Arg Tyr Gln Glu Gly Leu Arg Ala Leu 
305                 310                 315                 320 


His Arg Asp Gly Ala Lys Ile Ala Met Met Asn Tyr Ser Glu Phe Glu 
                325                 330                 335     


Tyr Cys Trp Asp Thr Phe Val Asp Arg Gln Gly Arg Pro Phe Gln Pro 
            340                 345                 350         


Trp Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg 
        355                 360                 365             


Ala Ile 
    370 


<210>  35
<211>  384
<212>  PRT
<213>  Pan troglodytes


<220>
<221>  MISC_FEATURE
<222>  (1)..(384)
<223>  Chimpanzee APOBEC-3G polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(60)
<223>  Cytoplasmic localization signal

<220>
<221>  MISC_FEATURE
<222>  (65)..(100)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (257)..(291)
<223>  Nucleic acid editing domain

<400>  35

Met Lys Pro His Phe Arg Asn Pro Val Glu Arg Met Tyr Gln Asp Thr 
1               5                   10                  15      


Phe Ser Asp Asn Phe Tyr Asn Arg Pro Ile Leu Ser His Arg Asn Thr 
            20                  25                  30          


Val Trp Leu Cys Tyr Glu Val Lys Thr Lys Gly Pro Ser Arg Pro Pro 
        35                  40                  45              


Leu Asp Ala Lys Ile Phe Arg Gly Gln Val Tyr Ser Lys Leu Lys Tyr 
    50                  55                  60                  


His Pro Glu Met Arg Phe Phe His Trp Phe Ser Lys Trp Arg Lys Leu 
65                  70                  75                  80  


His Arg Asp Gln Glu Tyr Glu Val Thr Trp Tyr Ile Ser Trp Ser Pro 
                85                  90                  95      


Cys Thr Lys Cys Thr Arg Asp Val Ala Thr Phe Leu Ala Glu Asp Pro 
            100                 105                 110         


Lys Val Thr Leu Thr Ile Phe Val Ala Arg Leu Tyr Tyr Phe Trp Asp 
        115                 120                 125             


Pro Asp Tyr Gln Glu Ala Leu Arg Ser Leu Cys Gln Lys Arg Asp Gly 
    130                 135                 140                 


Pro Arg Ala Thr Met Lys Ile Met Asn Tyr Asp Glu Phe Gln His Cys 
145                 150                 155                 160 


Trp Ser Lys Phe Val Tyr Ser Gln Arg Glu Leu Phe Glu Pro Trp Asn 
                165                 170                 175     


Asn Leu Pro Lys Tyr Tyr Ile Leu Leu His Ile Met Leu Gly Glu Ile 
            180                 185                 190         


Leu Arg His Ser Met Asp Pro Pro Thr Phe Thr Ser Asn Phe Asn Asn 
        195                 200                 205             


Glu Leu Trp Val Arg Gly Arg His Glu Thr Tyr Leu Cys Tyr Glu Val 
    210                 215                 220                 


Glu Arg Leu His Asn Asp Thr Trp Val Leu Leu Asn Gln Arg Arg Gly 
225                 230                 235                 240 


Phe Leu Cys Asn Gln Ala Pro His Lys His Gly Phe Leu Glu Gly Arg 
                245                 250                 255     


His Ala Glu Leu Cys Phe Leu Asp Val Ile Pro Phe Trp Lys Leu Asp 
            260                 265                 270         


Leu His Gln Asp Tyr Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys 
        275                 280                 285             


Phe Ser Cys Ala Gln Glu Met Ala Lys Phe Ile Ser Asn Asn Lys His 
    290                 295                 300                 


Val Ser Leu Cys Ile Phe Ala Ala Arg Ile Tyr Asp Asp Gln Gly Arg 
305                 310                 315                 320 


Cys Gln Glu Gly Leu Arg Thr Leu Ala Lys Ala Gly Ala Lys Ile Ser 
                325                 330                 335     


Ile Met Thr Tyr Ser Glu Phe Lys His Cys Trp Asp Thr Phe Val Asp 
            340                 345                 350         


His Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Glu Glu His Ser 
        355                 360                 365             


Gln Ala Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn Gln Gly Asn 
    370                 375                 380                 


<210>  36
<211>  377
<212>  PRT
<213>  Chlorocebus sabaeus


<220>
<221>  MISC_FEATURE
<222>  (1)..(377)
<223>  Green monkey APOBEC-3G polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(60)
<223>  Cytoplasmic localization signal

<220>
<221>  MISC_FEATURE
<222>  (65)..(100)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (257)..(290)
<223>  Nucleic acid editing domain

<400>  36

Met Asn Pro Gln Ile Arg Asn Met Val Glu Gln Met Glu Pro Asp Ile 
1               5                   10                  15      


Phe Val Tyr Tyr Phe Asn Asn Arg Pro Ile Leu Ser Gly Arg Asn Thr 
            20                  25                  30          


Val Trp Leu Cys Tyr Glu Val Lys Thr Lys Asp Pro Ser Gly Pro Pro 
        35                  40                  45              


Leu Asp Ala Asn Ile Phe Gln Gly Lys Leu Tyr Pro Glu Ala Lys Asp 
    50                  55                  60                  


His Pro Glu Met Lys Phe Leu His Trp Phe Arg Lys Trp Arg Gln Leu 
65                  70                  75                  80  


His Arg Asp Gln Glu Tyr Glu Val Thr Trp Tyr Val Ser Trp Ser Pro 
                85                  90                  95      


Cys Thr Arg Cys Ala Asn Ser Val Ala Thr Phe Leu Ala Glu Asp Pro 
            100                 105                 110         


Lys Val Thr Leu Thr Ile Phe Val Ala Arg Leu Tyr Tyr Phe Trp Lys 
        115                 120                 125             


Pro Asp Tyr Gln Gln Ala Leu Arg Ile Leu Cys Gln Glu Arg Gly Gly 
    130                 135                 140                 


Pro His Ala Thr Met Lys Ile Met Asn Tyr Asn Glu Phe Gln His Cys 
145                 150                 155                 160 


Trp Asn Glu Phe Val Asp Gly Gln Gly Lys Pro Phe Lys Pro Arg Lys 
                165                 170                 175     


Asn Leu Pro Lys His Tyr Thr Leu Leu His Ala Thr Leu Gly Glu Leu 
            180                 185                 190         


Leu Arg His Val Met Asp Pro Gly Thr Phe Thr Ser Asn Phe Asn Asn 
        195                 200                 205             


Lys Pro Trp Val Ser Gly Gln Arg Glu Thr Tyr Leu Cys Tyr Lys Val 
    210                 215                 220                 


Glu Arg Ser His Asn Asp Thr Trp Val Leu Leu Asn Gln His Arg Gly 
225                 230                 235                 240 


Phe Leu Arg Asn Gln Ala Pro Asp Arg His Gly Phe Pro Lys Gly Arg 
                245                 250                 255     


His Ala Glu Leu Cys Phe Leu Asp Leu Ile Pro Phe Trp Lys Leu Asp 
            260                 265                 270         


Asp Gln Gln Tyr Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys Phe 
        275                 280                 285             


Ser Cys Ala Gln Lys Met Ala Lys Phe Ile Ser Asn Asn Lys His Val 
    290                 295                 300                 


Ser Leu Cys Ile Phe Ala Ala Arg Ile Tyr Asp Asp Gln Gly Arg Cys 
305                 310                 315                 320 


Gln Glu Gly Leu Arg Thr Leu His Arg Asp Gly Ala Lys Ile Ala Val 
                325                 330                 335     


Met Asn Tyr Ser Glu Phe Glu Tyr Cys Trp Asp Thr Phe Val Asp Arg 
            340                 345                 350         


Gln Gly Arg Pro Phe Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln 
        355                 360                 365             


Ala Leu Ser Gly Arg Leu Arg Ala Ile 
    370                 375         


<210>  37
<211>  384
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(384)
<223>  Human APOBEC-3G polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(60)
<223>  Cytoplasmic localization signal

<220>
<221>  MISC_FEATURE
<222>  (65)..(100)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (257)..(291)
<223>  Nucleic acid editing domain

<400>  37

Met Lys Pro His Phe Arg Asn Thr Val Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Ser Tyr Asn Phe Tyr Asn Arg Pro Ile Leu Ser Arg Arg Asn Thr 
            20                  25                  30          


Val Trp Leu Cys Tyr Glu Val Lys Thr Lys Gly Pro Ser Arg Pro Pro 
        35                  40                  45              


Leu Asp Ala Lys Ile Phe Arg Gly Gln Val Tyr Ser Glu Leu Lys Tyr 
    50                  55                  60                  


His Pro Glu Met Arg Phe Phe His Trp Phe Ser Lys Trp Arg Lys Leu 
65                  70                  75                  80  


His Arg Asp Gln Glu Tyr Glu Val Thr Trp Tyr Ile Ser Trp Ser Pro 
                85                  90                  95      


Cys Thr Lys Cys Thr Arg Asp Met Ala Thr Phe Leu Ala Glu Asp Pro 
            100                 105                 110         


Lys Val Thr Leu Thr Ile Phe Val Ala Arg Leu Tyr Tyr Phe Trp Asp 
        115                 120                 125             


Pro Asp Tyr Gln Glu Ala Leu Arg Ser Leu Cys Gln Lys Arg Asp Gly 
    130                 135                 140                 


Pro Arg Ala Thr Met Lys Ile Met Asn Tyr Asp Glu Phe Gln His Cys 
145                 150                 155                 160 


Trp Ser Lys Phe Val Tyr Ser Gln Arg Glu Leu Phe Glu Pro Trp Asn 
                165                 170                 175     


Asn Leu Pro Lys Tyr Tyr Ile Leu Leu His Ile Met Leu Gly Glu Ile 
            180                 185                 190         


Leu Arg His Ser Met Asp Pro Pro Thr Phe Thr Phe Asn Phe Asn Asn 
        195                 200                 205             


Glu Pro Trp Val Arg Gly Arg His Glu Thr Tyr Leu Cys Tyr Glu Val 
    210                 215                 220                 


Glu Arg Met His Asn Asp Thr Trp Val Leu Leu Asn Gln Arg Arg Gly 
225                 230                 235                 240 


Phe Leu Cys Asn Gln Ala Pro His Lys His Gly Phe Leu Glu Gly Arg 
                245                 250                 255     


His Ala Glu Leu Cys Phe Leu Asp Val Ile Pro Phe Trp Lys Leu Asp 
            260                 265                 270         


Leu Asp Gln Asp Tyr Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys 
        275                 280                 285             


Phe Ser Cys Ala Gln Glu Met Ala Lys Phe Ile Ser Lys Asn Lys His 
    290                 295                 300                 


Val Ser Leu Cys Ile Phe Thr Ala Arg Ile Tyr Asp Asp Gln Gly Arg 
305                 310                 315                 320 


Cys Gln Glu Gly Leu Arg Thr Leu Ala Glu Ala Gly Ala Lys Ile Ser 
                325                 330                 335     


Ile Met Thr Tyr Ser Glu Phe Lys His Cys Trp Asp Thr Phe Val Asp 
            340                 345                 350         


His Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp Glu His Ser 
        355                 360                 365             


Gln Asp Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn Gln Glu Asn 
    370                 375                 380                 


<210>  38
<211>  382
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(382)
<223>  Human APOBEC-3B polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (66)..(100)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (251)..(289)
<223>  Nucleic acid editing domain

<400>  38

Met Asn Pro Gln Ile Arg Asn Pro Met Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Tyr Asp Asn Phe Glu Asn Glu Pro Ile Leu Tyr Gly Arg Ser Tyr 
            20                  25                  30          


Thr Trp Leu Cys Tyr Glu Val Lys Ile Lys Arg Gly Arg Ser Asn Leu 
        35                  40                  45              


Leu Trp Asp Thr Gly Val Phe Arg Gly Gln Val Tyr Phe Lys Pro Gln 
    50                  55                  60                  


Tyr His Ala Glu Met Cys Phe Leu Ser Trp Phe Cys Gly Asn Gln Leu 
65                  70                  75                  80  


Pro Ala Tyr Lys Cys Phe Gln Ile Thr Trp Phe Val Ser Trp Thr Pro 
                85                  90                  95      


Cys Pro Asp Cys Val Ala Lys Leu Ala Glu Phe Leu Ser Glu His Pro 
            100                 105                 110         


Asn Val Thr Leu Thr Ile Ser Ala Ala Arg Leu Tyr Tyr Tyr Trp Glu 
        115                 120                 125             


Arg Asp Tyr Arg Arg Ala Leu Cys Arg Leu Ser Gln Ala Gly Ala Arg 
    130                 135                 140                 


Val Thr Ile Met Asp Tyr Glu Glu Phe Ala Tyr Cys Trp Glu Asn Phe 
145                 150                 155                 160 


Val Tyr Asn Glu Gly Gln Gln Phe Met Pro Trp Tyr Lys Phe Asp Glu 
                165                 170                 175     


Asn Tyr Ala Phe Leu His Arg Thr Leu Lys Glu Ile Leu Arg Tyr Leu 
            180                 185                 190         


Met Asp Pro Asp Thr Phe Thr Phe Asn Phe Asn Asn Asp Pro Leu Val 
        195                 200                 205             


Leu Arg Arg Arg Gln Thr Tyr Leu Cys Tyr Glu Val Glu Arg Leu Asp 
    210                 215                 220                 


Asn Gly Thr Trp Val Leu Met Asp Gln His Met Gly Phe Leu Cys Asn 
225                 230                 235                 240 


Glu Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly Arg His Ala Glu Leu 
                245                 250                 255     


Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu Asp Pro Ala Gln Ile 
            260                 265                 270         


Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys Phe Ser Trp Gly 
        275                 280                 285             


Cys Ala Gly Glu Val Arg Ala Phe Leu Gln Glu Asn Thr His Val Arg 
    290                 295                 300                 


Leu Arg Ile Phe Ala Ala Arg Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys 
305                 310                 315                 320 


Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala Gln Val Ser Ile Met 
                325                 330                 335     


Thr Tyr Asp Glu Phe Glu Tyr Cys Trp Asp Thr Phe Val Tyr Arg Gln 
            340                 345                 350         


Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Glu Glu His Ser Gln Ala 
        355                 360                 365             


Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn Gln Gly Asn 
    370                 375                 380         


<210>  39
<211>  395
<212>  PRT
<213>  Rattus


<220>
<221>  MISC_FEATURE
<222>  (1)..(395)
<223>  Rat APOBEC-3B polypeptide sequence

<400>  39

Met Gln Pro Gln Gly Leu Gly Pro Asn Ala Gly Met Gly Pro Val Cys 
1               5                   10                  15      


Leu Gly Cys Ser His Arg Arg Pro Tyr Ser Pro Ile Arg Asn Pro Leu 
            20                  25                  30          


Lys Lys Leu Tyr Gln Gln Thr Phe Tyr Phe His Phe Lys Asn Val Arg 
        35                  40                  45              


Tyr Ala Trp Gly Arg Lys Asn Asn Phe Leu Cys Tyr Glu Val Asn Gly 
    50                  55                  60                  


Met Asp Cys Ala Leu Pro Val Pro Leu Arg Gln Gly Val Phe Arg Lys 
65                  70                  75                  80  


Gln Gly His Ile His Ala Glu Leu Cys Phe Ile Tyr Trp Phe His Asp 
                85                  90                  95      


Lys Val Leu Arg Val Leu Ser Pro Met Glu Glu Phe Lys Val Thr Trp 
            100                 105                 110         


Tyr Met Ser Trp Ser Pro Cys Ser Lys Cys Ala Glu Gln Val Ala Arg 
        115                 120                 125             


Phe Leu Ala Ala His Arg Asn Leu Ser Leu Ala Ile Phe Ser Ser Arg 
    130                 135                 140                 


Leu Tyr Tyr Tyr Leu Arg Asn Pro Asn Tyr Gln Gln Lys Leu Cys Arg 
145                 150                 155                 160 


Leu Ile Gln Glu Gly Val His Val Ala Ala Met Asp Leu Pro Glu Phe 
                165                 170                 175     


Lys Lys Cys Trp Asn Lys Phe Val Asp Asn Asp Gly Gln Pro Phe Arg 
            180                 185                 190         


Pro Trp Met Arg Leu Arg Ile Asn Phe Ser Phe Tyr Asp Cys Lys Leu 
        195                 200                 205             


Gln Glu Ile Phe Ser Arg Met Asn Leu Leu Arg Glu Asp Val Phe Tyr 
    210                 215                 220                 


Leu Gln Phe Asn Asn Ser His Arg Val Lys Pro Val Gln Asn Arg Tyr 
225                 230                 235                 240 


Tyr Arg Arg Lys Ser Tyr Leu Cys Tyr Gln Leu Glu Arg Ala Asn Gly 
                245                 250                 255     


Gln Glu Pro Leu Lys Gly Tyr Leu Leu Tyr Lys Lys Gly Glu Gln His 
            260                 265                 270         


Val Glu Ile Leu Phe Leu Glu Lys Met Arg Ser Met Glu Leu Ser Gln 
        275                 280                 285             


Val Arg Ile Thr Cys Tyr Leu Thr Trp Ser Pro Cys Pro Asn Cys Ala 
    290                 295                 300                 


Arg Gln Leu Ala Ala Phe Lys Lys Asp His Pro Asp Leu Ile Leu Arg 
305                 310                 315                 320 


Ile Tyr Thr Ser Arg Leu Tyr Phe Trp Arg Lys Lys Phe Gln Lys Gly 
                325                 330                 335     


Leu Cys Thr Leu Trp Arg Ser Gly Ile His Val Asp Val Met Asp Leu 
            340                 345                 350         


Pro Gln Phe Ala Asp Cys Trp Thr Asn Phe Val Asn Pro Gln Arg Pro 
        355                 360                 365             


Phe Arg Pro Trp Asn Glu Leu Glu Lys Asn Ser Trp Arg Ile Gln Arg 
    370                 375                 380                 


Arg Leu Arg Arg Ile Lys Glu Ser Trp Gly Leu 
385                 390                 395 


<210>  40
<211>  226
<212>  PRT
<213>  Bos Taurus


<220>
<221>  MISC_FEATURE
<222>  (1)..(226)
<223>  Bovine APOBEC-3B polypeptide sequence

<400>  40

Asp Gly Trp Glu Val Ala Phe Arg Ser Gly Thr Val Leu Lys Ala Gly 
1               5                   10                  15      


Val Leu Gly Val Ser Met Thr Glu Gly Trp Ala Gly Ser Gly His Pro 
            20                  25                  30          


Gly Gln Gly Ala Cys Val Trp Thr Pro Gly Thr Arg Asn Thr Met Asn 
        35                  40                  45              


Leu Leu Arg Glu Val Leu Phe Lys Gln Gln Phe Gly Asn Gln Pro Arg 
    50                  55                  60                  


Val Pro Ala Pro Tyr Tyr Arg Arg Lys Thr Tyr Leu Cys Tyr Gln Leu 
65                  70                  75                  80  


Lys Gln Arg Asn Asp Leu Thr Leu Asp Arg Gly Cys Phe Arg Asn Lys 
                85                  90                  95      


Lys Gln Arg His Ala Glu Arg Phe Ile Asp Lys Ile Asn Ser Leu Asp 
            100                 105                 110         


Leu Asn Pro Ser Gln Ser Tyr Lys Ile Ile Cys Tyr Ile Thr Trp Ser 
        115                 120                 125             


Pro Cys Pro Asn Cys Ala Asn Glu Leu Val Asn Phe Ile Thr Arg Asn 
    130                 135                 140                 


Asn His Leu Lys Leu Glu Ile Phe Ala Ser Arg Leu Tyr Phe His Trp 
145                 150                 155                 160 


Ile Lys Ser Phe Lys Met Gly Leu Gln Asp Leu Gln Asn Ala Gly Ile 
                165                 170                 175     


Ser Val Ala Val Met Thr His Thr Glu Phe Glu Asp Cys Trp Glu Gln 
            180                 185                 190         


Phe Val Asp Asn Gln Ser Arg Pro Phe Gln Pro Trp Asp Lys Leu Glu 
        195                 200                 205             


Gln Tyr Ser Ala Ser Ile Arg Arg Arg Leu Gln Arg Ile Leu Thr Ala 
    210                 215                 220                 


Pro Ile 
225     


<210>  41
<211>  490
<212>  PRT
<213>  Pan troglodytes


<220>
<221>  MISC_FEATURE
<222>  (1)..(490)
<223>  Chimpanzee APOBEC-3B polypeptide sequence

<400>  41

Met Asn Pro Gln Ile Arg Asn Pro Met Glu Trp Met Tyr Gln Arg Thr 
1               5                   10                  15      


Phe Tyr Tyr Asn Phe Glu Asn Glu Pro Ile Leu Tyr Gly Arg Ser Tyr 
            20                  25                  30          


Thr Trp Leu Cys Tyr Glu Val Lys Ile Arg Arg Gly His Ser Asn Leu 
        35                  40                  45              


Leu Trp Asp Thr Gly Val Phe Arg Gly Gln Met Tyr Ser Gln Pro Glu 
    50                  55                  60                  


His His Ala Glu Met Cys Phe Leu Ser Trp Phe Cys Gly Asn Gln Leu 
65                  70                  75                  80  


Ser Ala Tyr Lys Cys Phe Gln Ile Thr Trp Phe Val Ser Trp Thr Pro 
                85                  90                  95      


Cys Pro Asp Cys Val Ala Lys Leu Ala Lys Phe Leu Ala Glu His Pro 
            100                 105                 110         


Asn Val Thr Leu Thr Ile Ser Ala Ala Arg Leu Tyr Tyr Tyr Trp Glu 
        115                 120                 125             


Arg Asp Tyr Arg Arg Ala Leu Cys Arg Leu Ser Gln Ala Gly Ala Arg 
    130                 135                 140                 


Val Lys Ile Met Asp Asp Glu Glu Phe Ala Tyr Cys Trp Glu Asn Phe 
145                 150                 155                 160 


Val Tyr Asn Glu Gly Gln Pro Phe Met Pro Trp Tyr Lys Phe Asp Asp 
                165                 170                 175     


Asn Tyr Ala Phe Leu His Arg Thr Leu Lys Glu Ile Ile Arg His Leu 
            180                 185                 190         


Met Asp Pro Asp Thr Phe Thr Phe Asn Phe Asn Asn Asp Pro Leu Val 
        195                 200                 205             


Leu Arg Arg His Gln Thr Tyr Leu Cys Tyr Glu Val Glu Arg Leu Asp 
    210                 215                 220                 


Asn Gly Thr Trp Val Leu Met Asp Gln His Met Gly Phe Leu Cys Asn 
225                 230                 235                 240 


Glu Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly Arg His Ala Glu Leu 
                245                 250                 255     


Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu Asp Pro Ala Gln Ile 
            260                 265                 270         


Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys Phe Ser Trp Gly 
        275                 280                 285             


Cys Ala Gly Gln Val Arg Ala Phe Leu Gln Glu Asn Thr His Val Arg 
    290                 295                 300                 


Leu Arg Ile Phe Ala Ala Arg Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys 
305                 310                 315                 320 


Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala Gln Val Ser Ile Met 
                325                 330                 335     


Thr Tyr Asp Glu Phe Glu Tyr Cys Trp Asp Thr Phe Val Tyr Arg Gln 
            340                 345                 350         


Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Glu Glu His Ser Gln Ala 
        355                 360                 365             


Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Val Arg Ala Ser Ser Leu 
    370                 375                 380                 


Cys Met Val Pro His Arg Pro Pro Pro Pro Pro Gln Ser Pro Gly Pro 
385                 390                 395                 400 


Cys Leu Pro Leu Cys Ser Glu Pro Pro Leu Gly Ser Leu Leu Pro Thr 
                405                 410                 415     


Gly Arg Pro Ala Pro Ser Leu Pro Phe Leu Leu Thr Ala Ser Phe Ser 
            420                 425                 430         


Phe Pro Pro Pro Ala Ser Leu Pro Pro Leu Pro Ser Leu Ser Leu Ser 
        435                 440                 445             


Pro Gly His Leu Pro Val Pro Ser Phe His Ser Leu Thr Ser Cys Ser 
    450                 455                 460                 


Ile Gln Pro Pro Cys Ser Ser Arg Ile Arg Glu Thr Glu Gly Trp Ala 
465                 470                 475                 480 


Ser Val Ser Lys Glu Gly Arg Asp Leu Gly 
                485                 490 


<210>  42
<211>  190
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(190)
<223>  Human APOBEC-3C polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (65)..(100)
<223>  Nucleic acid editing domain

<400>  42

Met Asn Pro Gln Ile Arg Asn Pro Met Lys Ala Met Tyr Pro Gly Thr 
1               5                   10                  15      


Phe Tyr Phe Gln Phe Lys Asn Leu Trp Glu Ala Asn Asp Arg Asn Glu 
            20                  25                  30          


Thr Trp Leu Cys Phe Thr Val Glu Gly Ile Lys Arg Arg Ser Val Val 
        35                  40                  45              


Ser Trp Lys Thr Gly Val Phe Arg Asn Gln Val Asp Ser Glu Thr His 
    50                  55                  60                  


Cys His Ala Glu Arg Cys Phe Leu Ser Trp Phe Cys Asp Asp Ile Leu 
65                  70                  75                  80  


Ser Pro Asn Thr Lys Tyr Gln Val Thr Trp Tyr Thr Ser Trp Ser Pro 
                85                  90                  95      


Cys Pro Asp Cys Ala Gly Glu Val Ala Glu Phe Leu Ala Arg His Ser 
            100                 105                 110         


Asn Val Asn Leu Thr Ile Phe Thr Ala Arg Leu Tyr Tyr Phe Gln Tyr 
        115                 120                 125             


Pro Cys Tyr Gln Glu Gly Leu Arg Ser Leu Ser Gln Glu Gly Val Ala 
    130                 135                 140                 


Val Glu Ile Met Asp Tyr Glu Asp Phe Lys Tyr Cys Trp Glu Asn Phe 
145                 150                 155                 160 


Val Tyr Asn Asp Asn Glu Pro Phe Lys Pro Trp Lys Gly Leu Lys Thr 
                165                 170                 175     


Asn Phe Arg Leu Leu Lys Arg Arg Leu Arg Glu Ser Leu Gln 
            180                 185                 190 


<210>  43
<211>  190
<212>  PRT
<213>  Gorilla


<220>
<221>  MISC_FEATURE
<222>  (1)..(190)
<223>  Gorilla APOBEC-3C polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (65)..(100)
<223>  Nucleic acid editing domain

<400>  43

Met Asn Pro Gln Ile Arg Asn Pro Met Lys Ala Met Tyr Pro Gly Thr 
1               5                   10                  15      


Phe Tyr Phe Gln Phe Lys Asn Leu Trp Glu Ala Asn Asp Arg Asn Glu 
            20                  25                  30          


Thr Trp Leu Cys Phe Thr Val Glu Gly Ile Lys Arg Arg Ser Val Val 
        35                  40                  45              


Ser Trp Lys Thr Gly Val Phe Arg Asn Gln Val Asp Ser Glu Thr His 
    50                  55                  60                  


Cys His Ala Glu Arg Cys Phe Leu Ser Trp Glu Cys Asp Asp Ile Leu 
65                  70                  75                  80  


Ser Pro Asn Thr Asn Tyr Gln Val Thr Trp Tyr Thr Ser Trp Ser Pro 
                85                  90                  95      


Cys Pro Glu Cys Ala Gly Glu Val Ala Glu Phe Leu Ala Arg His Ser 
            100                 105                 110         


Asn Val Asn Leu Thr Ile Phe Thr Ala Arg Leu Tyr Tyr Phe Gln Asp 
        115                 120                 125             


Thr Asp Tyr Gln Glu Gly Leu Arg Ser Leu Ser Gln Glu Gly Val Ala 
    130                 135                 140                 


Val Lys Ile Met Asp Tyr Lys Asp Phe Lys Tyr Cys Trp Glu Asn Phe 
145                 150                 155                 160 


Val Tyr Asn Asp Asp Glu Pro Phe Lys Pro Trp Lys Gly Leu Lys Tyr 
                165                 170                 175     


Asn Phe Arg Phe Leu Lys Arg Arg Leu Gln Glu Ile Leu Glu 
            180                 185                 190 


<210>  44
<211>  202
<212>  PRT
<213>  Rhesus macaque


<220>
<221>  MISC_FEATURE
<222>  (1)..(202)
<223>  Rhesus macaque APOBEC-3A polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (72)..(103)
<223>  Nucleic acid editing domain

<400>  44

Met Asp Gly Ser Pro Ala Ser Arg Pro Arg His Leu Met Asp Pro Asn 
1               5                   10                  15      


Thr Phe Thr Phe Asn Phe Asn Asn Asp Leu Ser Val Arg Gly Arg His 
            20                  25                  30          


Gln Thr Tyr Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Trp 
        35                  40                  45              


Val Pro Met Asp Glu Arg Arg Gly Phe Leu Cys Asn Lys Ala Lys Asn 
    50                  55                  60                  


Val Pro Cys Gly Asp Tyr Gly Cys His Val Glu Leu Arg Phe Leu Cys 
65                  70                  75                  80  


Glu Val Pro Ser Trp Gln Leu Asp Pro Ala Gln Thr Tyr Arg Val Thr 
                85                  90                  95      


Trp Phe Ile Ser Trp Ser Pro Cys Phe Arg Arg Gly Cys Ala Gly Gln 
            100                 105                 110         


Val Arg Val Phe Leu Gln Glu Asn Lys His Val Arg Leu Arg Ile Phe 
        115                 120                 125             


Ala Ala Arg Ile Tyr Asp Tyr Asp Pro Leu Tyr Gln Glu Ala Leu Arg 
    130                 135                 140                 


Thr Leu Arg Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Glu Glu 
145                 150                 155                 160 


Phe Lys His Cys Trp Asp Thr Phe Val Asp Arg Gln Gly Arg Pro Phe 
                165                 170                 175     


Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg 
            180                 185                 190         


Leu Arg Ala Ile Leu Gln Asn Gln Gly Asn 
        195                 200         


<210>  45
<211>  185
<212>  PRT
<213>  Bos Taurus


<220>
<221>  MISC_FEATURE
<222>  (1)..(185)
<223>  Bovine APOBEC-3A polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (56)..(86)
<223>  Nucleic acid editing domain

<400>  45

Met Asp Glu Tyr Thr Phe Thr Glu Asn Phe Asn Asn Gln Gly Trp Pro 
1               5                   10                  15      


Ser Lys Thr Tyr Leu Cys Tyr Glu Met Glu Arg Leu Asp Gly Asp Ala 
            20                  25                  30          


Thr Ile Pro Leu Asp Glu Tyr Lys Gly Phe Val Arg Asn Lys Gly Leu 
        35                  40                  45              


Asp Gln Pro Glu Lys Pro Cys His Ala Glu Leu Tyr Phe Leu Gly Lys 
    50                  55                  60                  


Ile His Ser Trp Asn Leu Asp Arg Asn Gln His Tyr Arg Leu Thr Cys 
65                  70                  75                  80  


Phe Ile Ser Trp Ser Pro Cys Tyr Asp Cys Ala Gln Lys Leu Thr Thr 
                85                  90                  95      


Phe Leu Lys Glu Asn His His Ile Ser Leu His Ile Leu Ala Ser Arg 
            100                 105                 110         


Ile Tyr Thr His Asn Arg Phe Gly Cys His Gln Ser Gly Leu Cys Glu 
        115                 120                 125             


Leu Gln Ala Ala Gly Ala Arg Ile Thr Ile Met Thr Phe Glu Asp Phe 
    130                 135                 140                 


Lys His Cys Trp Glu Thr Phe Val Asp His Lys Gly Lys Pro Phe Gln 
145                 150                 155                 160 


Pro Trp Glu Gly Leu Asn Val Lys Ser Gln Ala Leu Cys Thr Glu Leu 
                165                 170                 175     


Gln Ala Ile Leu Lys Thr Gln Gln Asn 
            180                 185 


<210>  46
<211>  200
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(200)
<223>  Human APOBEC-3H polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (54)..(88)
<223>  Nucleic acid editing domain

<400>  46

Met Ala Leu Leu Thr Ala Glu Thr Phe Arg Leu Gln Phe Asn Asn Lys 
1               5                   10                  15      


Arg Arg Leu Arg Arg Pro Tyr Tyr Pro Arg Lys Ala Leu Leu Cys Tyr 
            20                  25                  30          


Gln Leu Thr Pro Gln Asn Gly Ser Thr Pro Thr Arg Gly Tyr Phe Glu 
        35                  40                  45              


Asn Lys Lys Lys Cys His Ala Glu Ile Cys Phe Ile Asn Glu Ile Lys 
    50                  55                  60                  


Ser Met Gly Leu Asp Glu Thr Gln Cys Tyr Gln Val Thr Cys Tyr Leu 
65                  70                  75                  80  


Thr Trp Ser Pro Cys Ser Ser Cys Ala Trp Glu Leu Val Asp Phe Ile 
                85                  90                  95      


Lys Ala His Asp His Leu Asn Leu Gly Ile Phe Ala Ser Arg Leu Tyr 
            100                 105                 110         


Tyr His Trp Cys Lys Pro Gln Gln Lys Gly Leu Arg Leu Leu Cys Gly 
        115                 120                 125             


Ser Gln Val Pro Val Glu Val Met Gly Phe Pro Lys Phe Ala Asp Cys 
    130                 135                 140                 


Trp Glu Asn Phe Val Asp His Glu Lys Pro Leu Ser Phe Asn Pro Tyr 
145                 150                 155                 160 


Lys Met Leu Glu Glu Leu Asp Lys Asn Ser Arg Ala Ile Lys Arg Arg 
                165                 170                 175     


Leu Glu Arg Ile Lys Ile Pro Gly Val Arg Ala Gln Gly Arg Tyr Met 
            180                 185                 190         


Asp Ile Leu Cys Asp Ala Glu Val 
        195                 200 


<210>  47
<211>  210
<212>  PRT
<213>  Rhesus macaque


<220>
<221>  MISC_FEATURE
<222>  (1)..(210)
<223>  Rhesus macaque APOBEC-3H polypeptide sequence

<400>  47

Met Ala Leu Leu Thr Ala Lys Thr Phe Ser Leu Gln Phe Asn Asn Lys 
1               5                   10                  15      


Arg Arg Val Asn Lys Pro Tyr Tyr Pro Arg Lys Ala Leu Leu Cys Tyr 
            20                  25                  30          


Gln Leu Thr Pro Gln Asn Gly Ser Thr Pro Thr Arg Gly His Leu Lys 
        35                  40                  45              


Asn Lys Lys Lys Asp His Ala Glu Ile Arg Phe Ile Asn Lys Ile Lys 
    50                  55                  60                  


Ser Met Gly Leu Asp Glu Thr Gln Cys Tyr Gln Val Thr Cys Tyr Leu 
65                  70                  75                  80  


Thr Trp Ser Pro Cys Pro Ser Cys Ala Gly Glu Leu Val Asp Phe Ile 
                85                  90                  95      


Lys Ala His Arg His Leu Asn Leu Arg Ile Phe Ala Ser Arg Leu Tyr 
            100                 105                 110         


Tyr His Trp Arg Pro Asn Tyr Gln Glu Gly Leu Leu Leu Leu Cys Gly 
        115                 120                 125             


Ser Gln Val Pro Val Glu Val Met Gly Leu Pro Glu Phe Thr Asp Cys 
    130                 135                 140                 


Trp Glu Asn Phe Val Asp His Lys Glu Pro Pro Ser Phe Asn Pro Ser 
145                 150                 155                 160 


Glu Lys Leu Glu Glu Leu Asp Lys Asn Ser Gln Ala Ile Lys Arg Arg 
                165                 170                 175     


Leu Glu Arg Ile Lys Ser Arg Ser Val Asp Val Leu Glu Asn Gly Leu 
            180                 185                 190         


Arg Ser Leu Gln Leu Gly Pro Val Thr Pro Ser Ser Ser Ile Arg Asn 
        195                 200                 205             


Ser Arg 
    210 


<210>  48
<211>  386
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(386)
<223>  Human APOBEC-3D polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (78)..(109)
<223>  Nucleic acid editing domain

<220>
<221>  MISC_FEATURE
<222>  (262)..(296)
<223>  Nucleic acid editing domain

<400>  48

Met Asn Pro Gln Ile Arg Asn Pro Met Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Tyr Asp Asn Phe Glu Asn Glu Pro Ile Leu Tyr Gly Arg Ser Tyr 
            20                  25                  30          


Thr Trp Leu Cys Tyr Glu Val Lys Ile Lys Arg Gly Arg Ser Asn Leu 
        35                  40                  45              


Leu Trp Asp Thr Gly Val Phe Arg Gly Pro Val Leu Pro Lys Arg Gln 
    50                  55                  60                  


Ser Asn His Arg Gln Glu Val Tyr Phe Arg Phe Glu Asn His Ala Glu 
65                  70                  75                  80  


Met Cys Phe Leu Ser Trp Phe Cys Gly Asn Arg Leu Pro Ala Asn Arg 
                85                  90                  95      


Arg Phe Gln Ile Thr Trp Phe Val Ser Trp Asn Pro Cys Leu Pro Cys 
            100                 105                 110         


Val Val Lys Val Thr Lys Phe Leu Ala Glu His Pro Asn Val Thr Leu 
        115                 120                 125             


Thr Ile Ser Ala Ala Arg Leu Tyr Tyr Tyr Arg Asp Arg Asp Trp Arg 
    130                 135                 140                 


Trp Val Leu Leu Arg Leu His Lys Ala Gly Ala Arg Val Lys Ile Met 
145                 150                 155                 160 


Asp Tyr Glu Asp Phe Ala Tyr Cys Trp Glu Asn Phe Val Cys Asn Glu 
                165                 170                 175     


Gly Gln Pro Phe Met Pro Trp Tyr Lys Phe Asp Asp Asn Tyr Ala Ser 
            180                 185                 190         


Leu His Arg Thr Leu Lys Glu Ile Leu Arg Asn Pro Met Glu Ala Met 
        195                 200                 205             


Tyr Pro His Ile Phe Tyr Phe His Phe Lys Asn Leu Leu Lys Ala Cys 
    210                 215                 220                 


Gly Arg Asn Glu Ser Trp Leu Cys Phe Thr Met Glu Val Thr Lys His 
225                 230                 235                 240 


His Ser Ala Val Phe Arg Lys Arg Gly Val Phe Arg Asn Gln Val Asp 
                245                 250                 255     


Pro Glu Thr His Cys His Ala Glu Arg Cys Phe Leu Ser Trp Phe Cys 
            260                 265                 270         


Asp Asp Ile Leu Ser Pro Asn Thr Asn Tyr Glu Val Thr Trp Tyr Thr 
        275                 280                 285             


Ser Trp Ser Pro Cys Pro Glu Cys Ala Gly Glu Val Ala Glu Phe Leu 
    290                 295                 300                 


Ala Arg His Ser Asn Val Asn Leu Thr Ile Phe Thr Ala Arg Leu Cys 
305                 310                 315                 320 


Tyr Phe Trp Asp Thr Asp Tyr Gln Glu Gly Leu Cys Ser Leu Ser Gln 
                325                 330                 335     


Glu Gly Ala Ser Val Lys Ile Met Gly Tyr Lys Asp Phe Val Ser Cys 
            340                 345                 350         


Trp Lys Asn Phe Val Tyr Ser Asp Asp Glu Pro Phe Lys Pro Trp Lys 
        355                 360                 365             


Gly Leu Gln Thr Asn Phe Arg Leu Leu Lys Arg Arg Leu Arg Glu Ile 
    370                 375                 380                 


Leu Gln 
385     


<210>  49
<211>  236
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(236)
<223>  Human APOBEC-1 polypeptide sequence

<400>  49

Met Thr Ser Glu Lys Gly Pro Ser Thr Gly Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro Trp Glu Phe Asp Val Phe Tyr Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Ala Cys Leu Leu Tyr Glu Ile Lys Trp Gly Met Ser Arg 
        35                  40                  45              


Lys Ile Trp Arg Ser Ser Gly Lys Asn Thr Thr Asn His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Lys Lys Phe Thr Ser Glu Arg Asp Phe His Pro Ser Met 
65                  70                  75                  80  


Ser Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu Cys 
                85                  90                  95      


Ser Gln Ala Ile Arg Glu Phe Leu Ser Arg His Pro Gly Val Thr Leu 
            100                 105                 110         


Val Ile Tyr Val Ala Arg Leu Phe Trp His Met Asp Gln Gln Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Val Asn Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Arg Ala Ser Glu Tyr Tyr His Cys Trp Arg Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Gly Asp Glu Ala His Trp Pro Gln Tyr Pro Pro Leu Trp Met Met 
                165                 170                 175     


Leu Tyr Ala Leu Glu Leu His Cys Ile Ile Leu Ser Leu Pro Pro Cys 
            180                 185                 190         


Leu Lys Ile Ser Arg Arg Trp Gln Asn His Leu Thr Phe Phe Arg Leu 
        195                 200                 205             


His Leu Gln Asn Cys His Tyr Gln Thr Ile Pro Pro His Ile Leu Leu 
    210                 215                 220                 


Ala Thr Gly Leu Ile His Pro Ser Val Ala Trp Arg 
225                 230                 235     


<210>  50
<211>  229
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(229)
<223>  Mouse APOBEC-1 polypeptide sequence

<400>  50

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Val Trp Arg His Thr Ser Gln Asn Thr Ser Asn His Val Glu Val 
    50                  55                  60                  


Asn Phe Leu Glu Lys Phe Thr Thr Glu Arg Tyr Phe Arg Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg His Pro Tyr Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Thr Asp Gln Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Tyr Cys Tyr Cys Trp Arg Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Ser Asn Glu Ala Tyr Trp Pro Arg Tyr Pro His Leu Trp Val Lys 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Lys Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Thr Leu Gln Thr Cys His Tyr Gln Arg Ile Pro Pro His Leu Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys 
225                 


<210>  51
<211>  224
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  Human APOBEC-2 polypeptide sequence

<400>  51

Met Ala Gln Lys Glu Glu Ala Ala Val Ala Thr Glu Ala Ala Ser Gln 
1               5                   10                  15      


Asn Gly Glu Asp Leu Glu Asn Leu Asp Asp Pro Glu Lys Leu Lys Glu 
            20                  25                  30          


Leu Ile Glu Leu Pro Pro Phe Glu Ile Val Thr Gly Glu Arg Leu Pro 
        35                  40                  45              


Ala Asn Phe Phe Lys Phe Gln Phe Arg Asn Val Glu Tyr Ser Ser Gly 
    50                  55                  60                  


Arg Asn Lys Thr Phe Leu Cys Tyr Val Val Glu Ala Gln Gly Lys Gly 
65                  70                  75                  80  


Gly Gln Val Gln Ala Ser Arg Gly Tyr Leu Glu Asp Glu His Ala Ala 
                85                  90                  95      


Ala His Ala Glu Glu Ala Phe Phe Asn Thr Ile Leu Pro Ala Phe Asp 
            100                 105                 110         


Pro Ala Leu Arg Tyr Asn Val Thr Trp Tyr Val Ser Ser Ser Pro Cys 
        115                 120                 125             


Ala Ala Cys Ala Asp Arg Ile Ile Lys Thr Leu Ser Lys Thr Lys Asn 
    130                 135                 140                 


Leu Arg Leu Leu Ile Leu Val Gly Arg Leu Phe Met Trp Glu Glu Pro 
145                 150                 155                 160 


Glu Ile Gln Ala Ala Leu Lys Lys Leu Lys Glu Ala Gly Cys Lys Leu 
                165                 170                 175     


Arg Ile Met Lys Pro Gln Asp Phe Glu Tyr Val Trp Gln Asn Phe Val 
            180                 185                 190         


Glu Gln Glu Glu Gly Glu Ser Lys Ala Phe Gln Pro Trp Glu Asp Ile 
        195                 200                 205             


Gln Glu Asn Phe Leu Tyr Tyr Glu Glu Lys Leu Ala Asp Ile Leu Lys 
    210                 215                 220                 


<210>  52
<211>  224
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  Mouse APOBEC-2 polypeptide sequence

<400>  52

Met Ala Gln Lys Glu Glu Ala Ala Glu Ala Ala Ala Pro Ala Ser Gln 
1               5                   10                  15      


Asn Gly Asp Asp Leu Glu Asn Leu Glu Asp Pro Glu Lys Leu Lys Glu 
            20                  25                  30          


Leu Ile Asp Leu Pro Pro Phe Glu Ile Val Thr Gly Val Arg Leu Pro 
        35                  40                  45              


Val Asn Phe Phe Lys Phe Gln Phe Arg Asn Val Glu Tyr Ser Ser Gly 
    50                  55                  60                  


Arg Asn Lys Thr Phe Leu Cys Tyr Val Val Glu Val Gln Ser Lys Gly 
65                  70                  75                  80  


Gly Gln Ala Gln Ala Thr Gln Gly Tyr Leu Glu Asp Glu His Ala Gly 
                85                  90                  95      


Ala His Ala Glu Glu Ala Phe Phe Asn Thr Ile Leu Pro Ala Phe Asp 
            100                 105                 110         


Pro Ala Leu Lys Tyr Asn Val Thr Trp Tyr Val Ser Ser Ser Pro Cys 
        115                 120                 125             


Ala Ala Cys Ala Asp Arg Ile Leu Lys Thr Leu Ser Lys Thr Lys Asn 
    130                 135                 140                 


Leu Arg Leu Leu Ile Leu Val Ser Arg Leu Phe Met Trp Glu Glu Pro 
145                 150                 155                 160 


Glu Val Gln Ala Ala Leu Lys Lys Leu Lys Glu Ala Gly Cys Lys Leu 
                165                 170                 175     


Arg Ile Met Lys Pro Gln Asp Phe Glu Tyr Ile Trp Gln Asn Phe Val 
            180                 185                 190         


Glu Gln Glu Glu Gly Glu Ser Lys Ala Phe Glu Pro Trp Glu Asp Ile 
        195                 200                 205             


Gln Glu Asn Phe Leu Tyr Tyr Glu Glu Lys Leu Ala Asp Ile Leu Lys 
    210                 215                 220                 


<210>  53
<211>  224
<212>  PRT
<213>  Rattus


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  Rat APOBEC-2 polypeptide sequence

<400>  53

Met Ala Gln Lys Glu Glu Ala Ala Glu Ala Ala Ala Pro Ala Ser Gln 
1               5                   10                  15      


Asn Gly Asp Asp Leu Glu Asn Leu Glu Asp Pro Glu Lys Leu Lys Glu 
            20                  25                  30          


Leu Ile Asp Leu Pro Pro Phe Glu Ile Val Thr Gly Val Arg Leu Pro 
        35                  40                  45              


Val Asn Phe Phe Lys Phe Gln Phe Arg Asn Val Glu Tyr Ser Ser Gly 
    50                  55                  60                  


Arg Asn Lys Thr Phe Leu Cys Tyr Val Val Glu Ala Gln Ser Lys Gly 
65                  70                  75                  80  


Gly Gln Val Gln Ala Thr Gln Gly Tyr Leu Glu Asp Glu His Ala Gly 
                85                  90                  95      


Ala His Ala Glu Glu Ala Phe Phe Asn Thr Ile Leu Pro Ala Phe Asp 
            100                 105                 110         


Pro Ala Leu Lys Tyr Asn Val Thr Trp Tyr Val Ser Ser Ser Pro Cys 
        115                 120                 125             


Ala Ala Cys Ala Asp Arg Ile Leu Lys Thr Leu Ser Lys Thr Lys Asn 
    130                 135                 140                 


Leu Arg Leu Leu Ile Leu Val Ser Arg Leu Phe Met Trp Glu Glu Pro 
145                 150                 155                 160 


Glu Val Gln Ala Ala Leu Lys Lys Leu Lys Glu Ala Gly Cys Lys Leu 
                165                 170                 175     


Arg Ile Met Lys Pro Gln Asp Phe Glu Tyr Leu Trp Gln Asn Phe Val 
            180                 185                 190         


Glu Gln Glu Glu Gly Glu Ser Lys Ala Phe Glu Pro Trp Glu Asp Ile 
        195                 200                 205             


Gln Glu Asn Phe Leu Tyr Tyr Glu Glu Lys Leu Ala Asp Ile Leu Lys 
    210                 215                 220                 


<210>  54
<211>  208
<212>  PRT
<213>  Petromyzon marinus


<220>
<221>  MISC_FEATURE
<222>  (1)..(208)
<223>  Petromyzon marinus CDA1 (pmCDAl) polypeptide sequence

<400>  54

Met Thr Asp Ala Glu Tyr Val Arg Ile His Glu Lys Leu Asp Ile Tyr 
1               5                   10                  15      


Thr Phe Lys Lys Gln Phe Phe Asn Asn Lys Lys Ser Val Ser His Arg 
            20                  25                  30          


Cys Tyr Val Leu Phe Glu Leu Lys Arg Arg Gly Glu Arg Arg Ala Cys 
        35                  40                  45              


Phe Trp Gly Tyr Ala Val Asn Lys Pro Gln Ser Gly Thr Glu Arg Gly 
    50                  55                  60                  


Ile His Ala Glu Ile Phe Ser Ile Arg Lys Val Glu Glu Tyr Leu Arg 
65                  70                  75                  80  


Asp Asn Pro Gly Gln Phe Thr Ile Asn Trp Tyr Ser Ser Trp Ser Pro 
                85                  90                  95      


Cys Ala Asp Cys Ala Glu Lys Ile Leu Glu Trp Tyr Asn Gln Glu Leu 
            100                 105                 110         


Arg Gly Asn Gly His Thr Leu Lys Ile Trp Ala Cys Lys Leu Tyr Tyr 
        115                 120                 125             


Glu Lys Asn Ala Arg Asn Gln Ile Gly Leu Trp Asn Leu Arg Asp Asn 
    130                 135                 140                 


Gly Val Gly Leu Asn Val Met Val Ser Glu His Tyr Gln Cys Cys Arg 
145                 150                 155                 160 


Lys Ile Phe Ile Gln Ser Ser His Asn Gln Leu Asn Glu Asn Arg Trp 
                165                 170                 175     


Leu Glu Lys Thr Leu Lys Arg Ala Glu Lys Arg Arg Ser Glu Leu Ser 
            180                 185                 190         


Phe Met Ile Gln Val Lys Ile Leu His Thr Thr Lys Ser Pro Ala Val 
        195                 200                 205             


<210>  55
<211>  381
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(381)
<223>  Human APOBEC3G D316R D317R polypeptide sequence

<400>  55

Met Lys Pro His Phe Arg Asn Thr Val Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Ser Tyr Asn Phe Tyr Asn Arg Pro Ile Leu Ser Arg Arg Asn Thr 
            20                  25                  30          


Val Trp Leu Cys Tyr Glu Val Lys Thr Lys Gly Pro Ser Arg Pro Pro 
        35                  40                  45              


Leu Asp Ala Lys Ile Phe Arg Gly Gln Val Tyr Ser Glu Leu Lys Tyr 
    50                  55                  60                  


His Pro Glu Met Arg Phe Phe His Trp Phe Ser Lys Trp Arg Lys Leu 
65                  70                  75                  80  


His Arg Asp Gln Glu Tyr Glu Val Thr Trp Tyr Ile Ser Trp Ser Pro 
                85                  90                  95      


Cys Thr Lys Cys Thr Arg Asp Met Ala Thr Phe Leu Ala Glu Asp Pro 
            100                 105                 110         


Lys Val Thr Leu Thr Ile Phe Val Ala Arg Leu Tyr Tyr Phe Trp Asp 
        115                 120                 125             


Pro Asp Tyr Gln Glu Ala Leu Arg Ser Leu Cys Gln Lys Arg Asp Gly 
    130                 135                 140                 


Pro Arg Ala Thr Met Lys Phe Asn Tyr Asp Glu Phe Gln His Cys Trp 
145                 150                 155                 160 


Ser Lys Phe Val Tyr Ser Gln Arg Glu Leu Phe Glu Pro Trp Asn Asn 
                165                 170                 175     


Leu Pro Lys Tyr Tyr Ile Leu Leu His Phe Met Leu Gly Glu Ile Leu 
            180                 185                 190         


Arg His Ser Met Asp Pro Pro Thr Phe Thr Phe Asn Phe Asn Asn Glu 
        195                 200                 205             


Pro Trp Val Arg Gly Arg His Glu Thr Tyr Leu Cys Tyr Glu Val Glu 
    210                 215                 220                 


Arg Met His Asn Asp Thr Trp Val Leu Leu Asn Gln Arg Arg Gly Phe 
225                 230                 235                 240 


Leu Cys Asn Gln Ala Pro His Lys His Gly Phe Leu Glu Gly Arg His 
                245                 250                 255     


Ala Glu Leu Cys Phe Leu Asp Val Ile Pro Phe Trp Lys Leu Asp Leu 
            260                 265                 270         


Asp Gln Asp Tyr Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys Phe 
        275                 280                 285             


Ser Cys Ala Gln Glu Met Ala Lys Phe Ile Ser Lys Lys His Val Ser 
    290                 295                 300                 


Leu Cys Ile Phe Thr Ala Arg Ile Tyr Arg Arg Gln Gly Arg Cys Gln 
305                 310                 315                 320 


Glu Gly Leu Arg Thr Leu Ala Glu Ala Gly Ala Lys Ile Ser Phe Thr 
                325                 330                 335     


Tyr Ser Glu Phe Lys His Cys Trp Asp Thr Phe Val Asp His Gln Gly 
            340                 345                 350         


Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln Asp Leu 
        355                 360                 365             


Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn Gln Glu Asn 
    370                 375                 380     


<210>  56
<211>  182
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(182)
<223>  Human APOBEC3G chain A polypeptide sequence

<400>  56

Met Asp Pro Pro Thr Phe Thr Phe Asn Phe Asn Asn Glu Pro Trp Trp 
1               5                   10                  15      


Gly Arg His Glu Thr Tyr Leu Cys Tyr Glu Val Glu Arg Met His Asn 
            20                  25                  30          


Asp Thr Trp Val Leu Leu Asn Gln Arg Arg Gly Phe Leu Cys Asn Gln 
        35                  40                  45              


Ala Pro His Lys His Gly Phe Leu Glu Gly Arg His Ala Glu Leu Cys 
    50                  55                  60                  


Phe Leu Asp Val Ile Pro Phe Trp Lys Leu Asp Leu Asp Gln Asp Tyr 
65                  70                  75                  80  


Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys Phe Ser Cys Ala Gln 
                85                  90                  95      


Glu Met Ala Lys Phe Ile Ser Lys Asn Lys His Val Ser Leu Cys Ile 
            100                 105                 110         


Phe Thr Ala Arg Ile Tyr Asp Asp Gln Gly Arg Cys Gln Glu Gly Leu 
        115                 120                 125             


Arg Thr Leu Ala Glu Ala Gly Ala Lys Ile Ser Phe Thr Tyr Ser Glu 
    130                 135                 140                 


Phe Lys His Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe 
145                 150                 155                 160 


Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln Asp Leu Ser Gly Arg 
                165                 170                 175     


Leu Arg Ala Ile Leu Gln 
            180         


<210>  57
<211>  184
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(184)
<223>  Human APOBEC3G chain A D120R D121R polypeptide sequence

<400>  57

Met Asp Pro Pro Thr Phe Thr Phe Asn Phe Asn Asn Glu Pro Trp Val 
1               5                   10                  15      


Arg Gly Arg His Glu Thr Tyr Leu Cys Tyr Glu Val Glu Arg Met His 
            20                  25                  30          


Asn Asp Thr Trp Val Leu Leu Asn Gln Arg Arg Gly Phe Leu Cys Asn 
        35                  40                  45              


Gln Ala Pro His Lys His Gly Phe Leu Glu Gly Arg His Ala Glu Leu 
    50                  55                  60                  


Cys Phe Leu Asp Val Ile Pro Phe Trp Lys Leu Asp Leu Asp Gln Asp 
65                  70                  75                  80  


Tyr Arg Val Thr Cys Phe Thr Ser Trp Ser Pro Cys Phe Ser Cys Ala 
                85                  90                  95      


Gln Glu Met Ala Lys Phe Ile Ser Lys Asn Lys His Val Ser Leu Cys 
            100                 105                 110         


Ile Phe Thr Ala Arg Ile Tyr Arg Arg Gln Gly Arg Cys Gln Glu Gly 
        115                 120                 125             


Leu Arg Thr Leu Ala Glu Ala Gly Ala Lys Ile Ser Phe Met Thr Tyr 
    130                 135                 140                 


Ser Glu Phe Lys His Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys 
145                 150                 155                 160 


Pro Phe Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln Asp Leu Ser 
                165                 170                 175     


Gly Arg Leu Arg Ala Ile Leu Gln 
            180                 


<210>  58
<211>  367
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(367)
<223>  hAPOBEC-4 polypeptide sequence

<400>  58

Met Glu Pro Ile Tyr Glu Glu Tyr Leu Ala Asn His Gly Thr Ile Val 
1               5                   10                  15      


Lys Pro Tyr Tyr Trp Leu Ser Phe Ser Leu Asp Cys Ser Asn Cys Pro 
            20                  25                  30          


Tyr His Ile Arg Thr Gly Glu Glu Ala Arg Val Ser Leu Thr Glu Phe 
        35                  40                  45              


Cys Gln Ile Phe Gly Phe Pro Tyr Gly Thr Thr Phe Pro Gln Thr Lys 
    50                  55                  60                  


His Leu Thr Phe Tyr Glu Leu Lys Thr Ser Ser Gly Ser Leu Val Gln 
65                  70                  75                  80  


Lys Gly His Ala Ser Ser Cys Thr Gly Asn Tyr Ile His Pro Glu Ser 
                85                  90                  95      


Met Leu Phe Glu Met Asn Gly Tyr Leu Asp Ser Ala Ile Tyr Asn Asn 
            100                 105                 110         


Asp Ser Ile Arg His Ile Ile Leu Tyr Ser Asn Asn Ser Pro Cys Asn 
        115                 120                 125             


Glu Ala Asn His Cys Cys Ile Ser Lys Met Tyr Asn Phe Leu Ile Thr 
    130                 135                 140                 


Tyr Pro Gly Ile Thr Leu Ser Ile Tyr Phe Ser Gln Leu Tyr His Thr 
145                 150                 155                 160 


Glu Met Asp Phe Pro Ala Ser Ala Trp Asn Arg Glu Ala Leu Arg Ser 
                165                 170                 175     


Leu Ala Ser Leu Trp Pro Arg Val Val Leu Ser Pro Ile Ser Gly Gly 
            180                 185                 190         


Ile Trp His Ser Val Leu His Ser Phe Ile Ser Gly Val Ser Gly Ser 
        195                 200                 205             


His Val Phe Gln Pro Ile Leu Thr Gly Arg Ala Leu Ala Asp Arg His 
    210                 215                 220                 


Asn Ala Tyr Glu Ile Asn Ala Ile Thr Gly Val Lys Pro Tyr Phe Thr 
225                 230                 235                 240 


Asp Val Leu Leu Gln Thr Lys Arg Asn Pro Asn Thr Lys Ala Gln Glu 
                245                 250                 255     


Ala Leu Glu Ser Tyr Pro Leu Asn Asn Ala Phe Pro Gly Gln Phe Phe 
            260                 265                 270         


Gln Met Pro Ser Gly Gln Leu Gln Pro Asn Leu Pro Pro Asp Leu Arg 
        275                 280                 285             


Ala Pro Val Val Phe Val Leu Val Pro Leu Arg Asp Leu Pro Pro Met 
    290                 295                 300                 


His Met Gly Gln Asn Pro Asn Lys Pro Arg Asn Ile Val Arg His Leu 
305                 310                 315                 320 


Asn Met Pro Gln Met Ser Phe Gln Glu Thr Lys Asp Leu Gly Arg Leu 
                325                 330                 335     


Pro Thr Gly Arg Ser Val Glu Ile Val Glu Ile Thr Glu Gln Phe Ala 
            340                 345                 350         


Ser Ser Lys Glu Ala Asp Glu Lys Lys Lys Lys Lys Gly Lys Lys 
        355                 360                 365         


<210>  59
<211>  388
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(388)
<223>  rAPOBEC-4 polypeptide sequence

<400>  59

Met Glu Pro Leu Tyr Glu Glu Tyr Leu Thr His Ser Gly Thr Ile Val 
1               5                   10                  15      


Lys Pro Tyr Tyr Trp Leu Ser Val Ser Leu Asn Cys Thr Asn Cys Pro 
            20                  25                  30          


Tyr His Ile Arg Thr Gly Glu Glu Ala Arg Val Pro Tyr Thr Glu Phe 
        35                  40                  45              


His Gln Thr Phe Gly Phe Pro Trp Ser Thr Tyr Pro Gln Thr Lys His 
    50                  55                  60                  


Leu Thr Phe Tyr Glu Leu Arg Ser Ser Ser Gly Asn Leu Ile Gln Lys 
65                  70                  75                  80  


Gly Leu Ala Ser Asn Cys Thr Gly Ser His Thr His Pro Glu Ser Met 
                85                  90                  95      


Leu Phe Glu Arg Asp Gly Tyr Leu Asp Ser Leu Ile Phe His Asp Ser 
            100                 105                 110         


Asn Ile Arg His Ile Ile Leu Tyr Ser Asn Asn Ser Pro Cys Asp Glu 
        115                 120                 125             


Ala Asn His Cys Cys Ile Ser Lys Met Tyr Asn Phe Leu Met Asn Tyr 
    130                 135                 140                 


Pro Glu Val Thr Leu Ser Val Phe Phe Ser Gln Leu Tyr His Thr Glu 
145                 150                 155                 160 


Asn Gln Phe Pro Thr Ser Ala Trp Asn Arg Glu Ala Leu Arg Gly Leu 
                165                 170                 175     


Ala Ser Leu Trp Pro Gln Val Thr Leu Ser Ala Ile Ser Gly Gly Ile 
            180                 185                 190         


Trp Gln Ser Ile Leu Glu Thr Phe Val Ser Gly Ile Ser Glu Gly Leu 
        195                 200                 205             


Thr Ala Val Arg Pro Phe Thr Ala Gly Arg Thr Leu Thr Asp Arg Tyr 
    210                 215                 220                 


Asn Ala Tyr Glu Ile Asn Cys Ile Thr Glu Val Lys Pro Tyr Phe Thr 
225                 230                 235                 240 


Asp Ala Leu His Ser Trp Gln Lys Glu Asn Gln Asp Gln Lys Val Trp 
                245                 250                 255     


Ala Ala Ser Glu Asn Gln Pro Leu His Asn Thr Thr Pro Ala Gln Trp 
            260                 265                 270         


Gln Pro Asp Met Ser Gln Asp Cys Arg Thr Pro Ala Val Phe Met Leu 
        275                 280                 285             


Val Pro Tyr Arg Asp Leu Pro Pro Ile His Val Asn Pro Ser Pro Gln 
    290                 295                 300                 


Lys Pro Arg Thr Val Val Arg His Leu Asn Thr Leu Gln Leu Ser Ala 
305                 310                 315                 320 


Ser Lys Val Lys Ala Leu Arg Lys Ser Pro Ser Gly Arg Pro Val Lys 
                325                 330                 335     


Lys Glu Glu Ala Arg Lys Gly Ser Thr Arg Ser Gln Glu Ala Asn Glu 
            340                 345                 350         


Thr Asn Lys Ser Lys Trp Lys Lys Gln Thr Leu Phe Ile Lys Ser Asn 
        355                 360                 365             


Ile Cys His Leu Leu Glu Arg Glu Gln Lys Lys Ile Gly Ile Leu Ser 
    370                 375                 380                 


Ser Trp Ser Val 
385             


<210>  60
<211>  363
<212>  PRT
<213>  Macaca fascicularis


<220>
<221>  MISC_FEATURE
<222>  (1)..(363)
<223>  mfAPOBEC-4 polypeptide sequence

<400>  60

Met Glu Pro Thr Tyr Glu Glu Tyr Leu Ala Asn His Gly Thr Ile Val 
1               5                   10                  15      


Lys Pro Tyr Tyr Trp Leu Ser Phe Ser Leu Asp Cys Ser Asn Cys Pro 
            20                  25                  30          


Tyr His Ile Arg Thr Gly Glu Glu Ala Arg Val Ser Leu Thr Glu Phe 
        35                  40                  45              


Cys Gln Ile Phe Gly Phe Pro Tyr Gly Thr Thr Tyr Pro Gln Thr Lys 
    50                  55                  60                  


His Leu Thr Phe Tyr Glu Leu Lys Thr Ser Ser Gly Ser Leu Val Gln 
65                  70                  75                  80  


Lys Gly His Ala Ser Ser Cys Thr Gly Asn Tyr Ile His Pro Glu Ser 
                85                  90                  95      


Met Leu Phe Glu Met Asn Gly Tyr Leu Asp Ser Ala Ile Tyr Asn Asn 
            100                 105                 110         


Asp Ser Ile Arg His Ile Ile Leu Tyr Cys Asn Asn Ser Pro Cys Asn 
        115                 120                 125             


Glu Ala Asn His Cys Cys Ile Ser Lys Val Tyr Asn Phe Leu Ile Thr 
    130                 135                 140                 


Tyr Pro Gly Ile Thr Leu Ser Ile Tyr Phe Ser Gln Leu Tyr His Thr 
145                 150                 155                 160 


Glu Met Asp Phe Pro Ala Ser Ala Trp Asn Arg Glu Ala Leu Arg Ser 
                165                 170                 175     


Leu Ala Ser Leu Trp Pro Arg Val Val Leu Ser Pro Ile Ser Gly Gly 
            180                 185                 190         


Ile Trp His Ser Val Leu His Ser Phe Val Ser Gly Val Ser Gly Ser 
        195                 200                 205             


His Val Phe Gln Pro Ile Leu Thr Gly Arg Ala Leu Thr Asp Arg Tyr 
    210                 215                 220                 


Asn Ala Tyr Glu Ile Asn Ala Ile Thr Gly Val Lys Pro Phe Phe Thr 
225                 230                 235                 240 


Asp Val Leu Leu His Thr Lys Arg Asn Pro Asn Thr Lys Ala Gln Met 
                245                 250                 255     


Ala Leu Glu Ser Tyr Pro Leu Asn Asn Ala Phe Pro Gly Gln Ser Phe 
            260                 265                 270         


Gln Met Thr Ser Gly Ile Pro Pro Asp Leu Arg Ala Pro Val Val Phe 
        275                 280                 285             


Val Leu Leu Pro Leu Arg Asp Leu Pro Pro Met His Met Gly Gln Asp 
    290                 295                 300                 


Pro Asn Lys Pro Arg Asn Ile Ile Arg His Leu Asn Met Pro Gln Met 
305                 310                 315                 320 


Ser Phe Gln Glu Thr Lys Asp Leu Glu Arg Leu Pro Thr Arg Arg Ser 
                325                 330                 335     


Val Glu Thr Val Glu Ile Thr Glu Arg Phe Ala Ser Ser Lys Gln Ala 
            340                 345                 350         


Glu Glu Lys Thr Lys Lys Lys Lys Gly Lys Lys 
        355                 360             


<210>  61
<211>  218
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(218)
<223>  rAPOBEC-1 (delta 177-186) polypeptide sequence

<400>  61

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu 
            180                 185                 190         


Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro 
        195                 200                 205             


Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
    210                 215             


<210>  62
<211>  218
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(218)
<223>  rAPOBEC-1 (delta 202-213) polypeptide sequence

<400>  62

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln His Tyr Gln Arg Leu Pro 
        195                 200                 205             


Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
    210                 215             


<210>  63
<211>  198
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(198)
<223>  mouse cytidine deaminase polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  Nuclear localization sequence

<220>
<221>  MISC_FEATURE
<222>  (183)..(198)
<223>  Nuclear export signal

<400>  63

Met Asp Ser Leu Leu Met Lys Gln Lys Lys Phe Leu Tyr His Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg His Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Cys Ser Leu Asp Phe Gly His 
        35                  40                  45              


Leu Arg Asn Lys Ser Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Glu 
                85                  90                  95      


Phe Leu Arg Trp Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Gly Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn Arg Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Thr Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Met Leu Gly Phe 
        195             


<210>  64
<211>  224
<212>  PRT
<213>  Petromyzon marinus


<220>
<221>  MISC_FEATURE
<222>  (1)..(224)
<223>  pmCDA-1 polypeptide sequence

<400>  64

Met Ala Gly Tyr Glu Cys Val Arg Val Ser Glu Lys Leu Asp Phe Asp 
1               5                   10                  15      


Thr Phe Glu Phe Gln Phe Glu Asn Leu His Tyr Ala Thr Glu Arg His 
            20                  25                  30          


Arg Thr Tyr Val Ile Phe Asp Val Lys Pro Gln Ser Ala Gly Gly Arg 
        35                  40                  45              


Ser Arg Arg Leu Trp Gly Tyr Ile Ile Asn Asn Pro Asn Val Cys His 
    50                  55                  60                  


Ala Glu Leu Ile Leu Met Ser Met Ile Asp Arg His Leu Glu Ser Asn 
65                  70                  75                  80  


Pro Gly Val Tyr Ala Met Thr Trp Tyr Met Ser Trp Ser Pro Cys Ala 
                85                  90                  95      


Asn Cys Ser Ser Lys Leu Asn Pro Trp Leu Lys Asn Leu Leu Glu Glu 
            100                 105                 110         


Gln Gly His Thr Leu Thr Met His Phe Ser Arg Ile Tyr Asp Arg Asp 
        115                 120                 125             


Arg Glu Gly Asp His Arg Gly Leu Arg Gly Leu Lys His Val Ser Asn 
    130                 135                 140                 


Ser Phe Arg Met Gly Val Val Gly Arg Ala Glu Val Lys Glu Cys Leu 
145                 150                 155                 160 


Ala Glu Tyr Val Glu Ala Ser Arg Arg Thr Leu Thr Trp Leu Asp Thr 
                165                 170                 175     


Thr Glu Ser Met Ala Ala Lys Met Arg Arg Lys Leu Phe Cys Ile Leu 
            180                 185                 190         


Val Arg Cys Ala Gly Met Arg Glu Ser Gly Ile Pro Leu His Leu Phe 
        195                 200                 205             


Thr Leu Gln Thr Pro Leu Leu Ser Gly Arg Val Val Trp Trp Arg Val 
    210                 215                 220                 


<210>  65
<211>  331
<212>  PRT
<213>  Petromyzon marinus


<220>
<221>  MISC_FEATURE
<222>  (1)..(331)
<223>  pmCDA-2 polypeptide sequence

<400>  65

Met Glu Leu Arg Glu Val Val Asp Cys Ala Leu Ala Ser Cys Val Arg 
1               5                   10                  15      


His Glu Pro Leu Ser Arg Val Ala Phe Leu Arg Cys Phe Ala Ala Pro 
            20                  25                  30          


Ser Gln Lys Pro Arg Gly Thr Val Ile Leu Phe Tyr Val Glu Gly Ala 
        35                  40                  45              


Gly Arg Gly Val Thr Gly Gly His Ala Val Asn Tyr Asn Lys Gln Gly 
    50                  55                  60                  


Thr Ser Ile His Ala Glu Val Leu Leu Leu Ser Ala Val Arg Ala Ala 
65                  70                  75                  80  


Leu Leu Arg Arg Arg Arg Cys Glu Asp Gly Glu Glu Ala Thr Arg Gly 
                85                  90                  95      


Cys Thr Leu His Cys Tyr Ser Thr Tyr Ser Pro Cys Arg Asp Cys Val 
            100                 105                 110         


Glu Tyr Ile Gln Glu Phe Gly Ala Ser Thr Gly Val Arg Val Val Ile 
        115                 120                 125             


His Cys Cys Arg Leu Tyr Glu Leu Asp Val Asn Arg Arg Arg Ser Glu 
    130                 135                 140                 


Ala Glu Gly Val Leu Arg Ser Leu Ser Arg Leu Gly Arg Asp Phe Arg 
145                 150                 155                 160 


Leu Met Gly Pro Arg Asp Ala Ile Ala Leu Leu Leu Gly Gly Arg Leu 
                165                 170                 175     


Ala Asn Thr Ala Asp Gly Glu Ser Gly Ala Ser Gly Asn Ala Trp Val 
            180                 185                 190         


Thr Glu Thr Asn Val Val Glu Pro Leu Val Asp Met Thr Gly Phe Gly 
        195                 200                 205             


Asp Glu Asp Leu His Ala Gln Val Gln Arg Asn Lys Gln Ile Arg Glu 
    210                 215                 220                 


Ala Tyr Ala Asn Tyr Ala Ser Ala Val Ser Leu Met Leu Gly Glu Leu 
225                 230                 235                 240 


His Val Asp Pro Asp Lys Phe Pro Phe Leu Ala Glu Phe Leu Ala Gln 
                245                 250                 255     


Thr Ser Val Glu Pro Ser Gly Thr Pro Arg Glu Thr Arg Gly Arg Pro 
            260                 265                 270         


Arg Gly Ala Ser Ser Arg Gly Pro Glu Ile Gly Arg Gln Arg Pro Ala 
        275                 280                 285             


Asp Phe Glu Arg Ala Leu Gly Ala Tyr Gly Leu Phe Leu His Pro Arg 
    290                 295                 300                 


Ile Val Ser Arg Glu Ala Asp Arg Glu Glu Ile Lys Arg Asp Leu Ile 
305                 310                 315                 320 


Val Val Met Arg Lys His Asn Tyr Gln Gly Pro 
                325                 330     


<210>  66
<211>  209
<212>  PRT
<213>  Petromyzon marinus


<220>
<221>  MISC_FEATURE
<222>  (1)..(209)
<223>  pmCDA-5  polypeptide sequence

<400>  66

Met Ala Gly Asp Glu Asn Val Arg Val Ser Glu Lys Leu Asp Phe Asp 
1               5                   10                  15      


Thr Phe Glu Phe Gln Phe Glu Asn Leu His Tyr Ala Thr Glu Arg His 
            20                  25                  30          


Arg Thr Tyr Val Ile Phe Asp Val Lys Pro Gln Ser Ala Gly Gly Arg 
        35                  40                  45              


Ser Arg Arg Leu Trp Gly Tyr Ile Ile Asn Asn Pro Asn Val Cys His 
    50                  55                  60                  


Ala Glu Leu Ile Leu Met Ser Met Ile Asp Arg His Leu Glu Ser Asn 
65                  70                  75                  80  


Pro Gly Val Tyr Ala Met Thr Trp Tyr Met Ser Trp Ser Pro Cys Ala 
                85                  90                  95      


Asn Cys Ser Ser Lys Leu Asn Pro Trp Leu Lys Asn Leu Leu Glu Glu 
            100                 105                 110         


Gln Gly His Thr Leu Met Met His Phe Ser Arg Ile Tyr Asp Arg Asp 
        115                 120                 125             


Arg Glu Gly Asp His Arg Gly Leu Arg Gly Leu Lys His Val Ser Asn 
    130                 135                 140                 


Ser Phe Arg Met Gly Val Val Gly Arg Ala Glu Val Lys Glu Cys Leu 
145                 150                 155                 160 


Ala Glu Tyr Val Glu Ala Ser Arg Arg Thr Leu Thr Trp Leu Asp Thr 
                165                 170                 175     


Thr Glu Ser Met Ala Ala Lys Met Arg Arg Lys Leu Phe Cys Ile Leu 
            180                 185                 190         


Val Arg Cys Ala Gly Met Arg Glu Ser Gly Met Pro Leu His Leu Phe 
        195                 200                 205             


Thr 
    


<210>  67
<211>  158
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<222>  (1)..(158)
<223>  yCD polypeptide sequence

<400>  67

Met Val Thr Gly Gly Met Ala Ser Lys Trp Asp Gln Lys Gly Met Asp 
1               5                   10                  15      


Ile Ala Tyr Glu Glu Ala Ala Leu Gly Tyr Lys Glu Gly Gly Val Pro 
            20                  25                  30          


Ile Gly Gly Cys Leu Ile Asn Asn Lys Asp Gly Ser Val Leu Gly Arg 
        35                  40                  45              


Gly His Asn Met Arg Phe Gln Lys Gly Ser Ala Thr Leu His Gly Glu 
    50                  55                  60                  


Ile Ser Thr Leu Glu Asn Cys Gly Arg Leu Glu Gly Lys Val Tyr Lys 
65                  70                  75                  80  


Asp Thr Thr Leu Tyr Thr Thr Leu Ser Pro Cys Asp Met Cys Thr Gly 
                85                  90                  95      


Ala Ile Ile Met Tyr Gly Ile Pro Arg Cys Val Val Gly Glu Asn Val 
            100                 105                 110         


Asn Phe Lys Ser Lys Gly Glu Lys Tyr Leu Gln Thr Arg Gly His Glu 
        115                 120                 125             


Val Val Val Val Asp Asp Glu Arg Cys Lys Lys Ile Met Lys Gln Phe 
    130                 135                 140                 


Ile Asp Glu Arg Pro Gln Asp Trp Phe Glu Asp Ile Gly Glu 
145                 150                 155             


<210>  68
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: cytidine deaminase 
       polypeptide sequence

<400>  68

Met Lys Pro Gln Ile Arg Asp His Arg Pro Asn Pro Met Glu Ala Met 
1               5                   10                  15      


Tyr Pro His Ile Phe Tyr Phe His Phe Glu Asn Leu Glu Lys Ala Tyr 
            20                  25                  30          


Gly Arg Asn Glu Thr Trp Leu Cys Phe Thr Val Glu Ile Ile Lys Gln 
        35                  40                  45              


Tyr Leu Pro Val Pro Trp Lys Lys Gly Val Phe Arg Asn Gln Val Asp 
    50                  55                  60                  


Pro Glu Thr His Cys His Ala Glu Lys Cys Phe Leu Ser Trp Phe Cys 
65                  70                  75                  80  


Asn Asn Thr Leu Ser Pro Lys Lys Asn Tyr Gln Val Thr Trp Tyr Thr 
                85                  90                  95      


Ser Trp Ser Pro Cys Pro Glu Cys Ala Gly Glu Val Ala Glu Phe Leu 
            100                 105                 110         


Ala Glu His Ser Asn Val Lys Leu Thr Ile Tyr Thr Ala Arg Leu Tyr 
        115                 120                 125             


Tyr Phe Trp Asp Thr Asp Tyr Gln Glu Gly Leu Arg Ser Leu Ser Glu 
    130                 135                 140                 


Glu Gly Ala Ser Val Glu Ile Met Asp Tyr Glu Asp Phe Gln Tyr Cys 
145                 150                 155                 160 


Trp Glu Asn Phe Val Tyr Asp Asp Gly Glu Pro Phe Lys Arg Trp Lys 
                165                 170                 175     


Gly Leu Lys Tyr Asn Phe Gln Ser Leu Thr Arg Arg Leu Arg Glu Ile 
            180                 185                 190         


Leu Gln 
        


<210>  69
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: cytidine deaminase 
       polypeptide sequence

<400>  69

Met Ala Asp Ser Ser Glu Lys Met Arg Gly Gln Tyr Ile Ser Arg Asp 
1               5                   10                  15      


Thr Phe Glu Lys Asn Tyr Lys Pro Ile Asp Gly Thr Lys Glu Ala His 
            20                  25                  30          


Leu Leu Cys Glu Ile Lys Trp Gly Lys Tyr Gly Lys Pro Trp Leu His 
        35                  40                  45              


Trp Cys Gln Asn Gln Arg Met Asn Ile His Ala Glu Asp Tyr Phe Met 
    50                  55                  60                  


Asn Asn Ile Phe Lys Ala Lys Lys His Pro Val His Cys Tyr Val Thr 
65                  70                  75                  80  


Trp Tyr Leu Ser Trp Ser Pro Cys Ala Asp Cys Ala Ser Lys Ile Val 
                85                  90                  95      


Lys Phe Leu Glu Glu Arg Pro Tyr Leu Lys Leu Thr Ile Tyr Val Ala 
            100                 105                 110         


Gln Leu Tyr Tyr His Thr Glu Glu Glu Asn Arg Lys Gly Leu Arg Leu 
        115                 120                 125             


Leu Arg Ser Lys Lys Val Ile Ile Arg Val Met Asp Ile Ser Asp Tyr 
    130                 135                 140                 


Asn Tyr Cys Trp Lys Val Phe Val Ser Asn Gln Asn Gly Asn Glu Asp 
145                 150                 155                 160 


Tyr Trp Pro Leu Gln Phe Asp Pro Trp Val Lys Glu Asn Tyr Ser Arg 
                165                 170                 175     


Leu Leu Asp Ile Phe Trp Glu Ser Lys Cys Arg Ser Pro Asn Pro Trp 
            180                 185                 190         


<210>  70
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: cytidine deaminase 
       polypeptide sequence

<400>  70

Met Asp Pro Gln Arg Leu Arg Gln Trp Pro Gly Pro Gly Pro Ala Ser 
1               5                   10                  15      


Arg Gly Gly Tyr Gly Gln Arg Pro Arg Ile Arg Asn Pro Glu Glu Trp 
            20                  25                  30          


Phe His Glu Leu Ser Pro Arg Thr Phe Ser Phe His Phe Arg Asn Leu 
        35                  40                  45              


Arg Phe Ala Ser Gly Arg Asn Arg Ser Tyr Ile Cys Cys Gln Val Glu 
    50                  55                  60                  


Gly Lys Asn Cys Phe Phe Gln Gly Ile Phe Gln Asn Gln Val Pro Pro 
65                  70                  75                  80  


Asp Pro Pro Cys His Ala Glu Leu Cys Phe Leu Ser Trp Phe Gln Ser 
                85                  90                  95      


Trp Gly Leu Ser Pro Asp Glu His Tyr Tyr Val Thr Trp Phe Ile Ser 
            100                 105                 110         


Trp Ser Pro Cys Cys Glu Cys Ala Ala Lys Val Ala Gln Phe Leu Glu 
        115                 120                 125             


Glu Asn Arg Asn Val Ser Leu Ser Leu Ser Ala Ala Arg Leu Tyr Tyr 
    130                 135                 140                 


Phe Trp Lys Ser Glu Ser Arg Glu Gly Leu Arg Arg Leu Ser Asp Leu 
145                 150                 155                 160 


Gly Ala Gln Val Gly Ile Met Ser Phe Gln Asp Phe Gln His Cys Trp 
                165                 170                 175     


Asn Asn Phe Val His Asn Leu Gly Met Pro Phe Gln Pro Trp Lys Lys 
            180                 185                 190         


Leu His Lys Asn Tyr Gln Arg Leu Val Thr Glu Leu Lys Gln Ile Leu 
        195                 200                 205             


Arg Glu Glu Pro Ala Thr Tyr Gly Ser Pro Gln Ala Gln Gly Lys Val 
    210                 215                 220                 


Arg Ile Gly Ser Thr Ala Ala Gly Leu Arg His Ser His Ser His Thr 
225                 230                 235                 240 


Arg Ser Glu Ala His Leu Arg Pro Asn His Ser Ser Arg Gln His Arg 
                245                 250                 255     


Ile Leu Asn Pro Pro Arg Glu Ala Arg Ala Arg Thr Cys Val Leu Val 
            260                 265                 270         


Asp Ala Ser Trp Ile Cys Tyr Arg 
        275                 280 


<210>  71
<211>  555
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.1; 
       tr|F7B644|F7B644_HORSE polypeptide sequence

<400>  71

Met Pro Arg Gly Arg Ala Arg Glu Arg Gln Arg Arg Asn Pro Met Glu 
1               5                   10                  15      


Lys Leu Asp Ala Glu Ala Phe Ser Phe His Phe Leu Asn Met Glu Phe 
            20                  25                  30          


Val Tyr Asp Arg Asn Cys Ser Tyr Leu Cys Tyr Gln Val Glu Gly Arg 
        35                  40                  45              


Leu Ser Gly Ser Pro Val Leu Ser Glu Gln Gly Val Phe Pro Asn Glu 
    50                  55                  60                  


Val Cys Gly Lys Thr Arg Arg His Ala Glu Leu Cys Phe Leu Asp Trp 
65                  70                  75                  80  


Phe Arg Gly Arg Leu Ser Pro Asp Glu Tyr Tyr Cys Val Thr Trp Phe 
                85                  90                  95      


Ile Ser Trp Ser Pro Cys Ser Asn Cys Ala Arg Glu Val Ala Glu Phe 
            100                 105                 110         


Leu Lys Arg His Arg Asn Val Glu Leu Ser Ile Phe Ala Ala Arg Leu 
        115                 120                 125             


Tyr Tyr Cys Arg Asp His Glu Gln Gly Leu Gln Ser Leu Cys Asn Arg 
    130                 135                 140                 


Gly Ala Gln Leu Ala Val Met Leu Arg Lys Asp Phe Thr Tyr Cys Trp 
145                 150                 155                 160 


Asp Asn Phe Val His Asn Ser Gly Arg Glu Phe Ser Pro Trp Glu Asn 
                165                 170                 175     


Ile Asp Ala Asn Ser Asp Leu Leu Ala Arg Lys Leu Glu Asp Leu Leu 
            180                 185                 190         


Lys Asn Pro Met Glu Lys Leu His Arg Lys Thr Phe Ser Phe His Phe 
        195                 200                 205             


Arg Asn Leu Lys Phe Ala Lys Gly Arg Lys Cys Ser Tyr Leu Cys Tyr 
    210                 215                 220                 


Arg Val Glu Gly Arg Leu Ser Gly Ser Pro Gly Leu Ser Glu Gln Gly 
225                 230                 235                 240 


Val Phe Leu Asn Glu Val Cys Asp Glu Asn Cys Arg His Ala Glu Leu 
                245                 250                 255     


Cys Phe Leu His Trp Phe Arg Gly Arg Leu Ser Pro His Ala Asp Tyr 
            260                 265                 270         


Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys Ser Asn Cys Ala Arg 
        275                 280                 285             


Glu Val Ala Glu Phe Leu Lys Gln His Arg Asn Val Glu Leu His Ile 
    290                 295                 300                 


Ser Ala Ala Arg Leu Tyr Tyr Trp Gln Arg Asn Lys Pro Gly Leu Arg 
305                 310                 315                 320 


Asn Leu Arg Ser Ser Gly Ala Gln Leu Ala Ile Met Phe Phe Trp Asp 
                325                 330                 335     


Phe Arg Asp Cys Trp Asp Asn Phe Val His Asn Ser Gly Arg His Phe 
            340                 345                 350         


Ile Pro Trp Lys Lys Ile Asn Val Asn Ser Arg Leu Leu Ala Thr Lys 
        355                 360                 365             


Leu Glu Asp Leu Leu Lys Asn Pro Leu Glu Lys Leu His Pro Asn Thr 
    370                 375                 380                 


Phe Ser Phe His Phe Cys Asn Leu Glu Phe Ala Tyr Asp Arg Lys Tyr 
385                 390                 395                 400 


Ser Tyr Leu Cys Tyr Gln Val Glu Gly Arg Leu Ser Gly Ser Pro Gly 
                405                 410                 415     


Leu Ser Glu Gln Gly Val Phe Leu Asn Glu Val Cys Gly Lys Thr Arg 
            420                 425                 430         


Cys His Ala Glu Leu Cys Phe Leu Asp Trp Phe Arg Val Arg Leu Ser 
        435                 440                 445             


Pro Asp Glu Tyr Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys 
    450                 455                 460                 


Phe Tyr Cys Ala Arg Glu Val Ala Asp Phe Leu Lys Gln Tyr Arg Asn 
465                 470                 475                 480 


Val Lys Leu Ser Ile Phe Ala Ala Arg Leu Tyr Tyr Cys Arg Asp His 
                485                 490                 495     


Ala Gln Gly Leu Arg Ser Leu Cys Ser Ser Gly Ala Gln Leu Ala Ile 
            500                 505                 510         


Met Phe Phe Trp Asp Phe Arg Tyr Cys Trp Asp Asn Phe Val His Asn 
        515                 520                 525             


Ser Gly Arg Glu Phe Arg Pro Trp Lys Lys Ile Asn Val Asn Ser Arg 
    530                 535                 540                 


Leu Leu Ala Thr Lys Leu Glu Asp Ile Leu Lys 
545                 550                 555 


<210>  72
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.2; 
       tr|D1LZA1|D1LZA1_PANTI polypeptide sequence

<400>  72

Met Glu Pro Trp Arg Pro Ser Pro Arg Asn Pro Met Asp Arg Ile Asp 
1               5                   10                  15      


Pro Lys Thr Phe Arg Phe Gln Phe Pro Asn Leu Arg Tyr Ala Ser Gly 
            20                  25                  30          


Arg Lys Leu Cys Tyr Leu Cys Phe Gln Val Glu Arg Asp Tyr Phe Tyr 
        35                  40                  45              


Tyr Asn Asp Ser Asp Trp Gly Val Phe Arg Asn Glu Val His Pro Trp 
    50                  55                  60                  


Ala Pro Cys His Ala Glu Gln Cys Phe Leu Ser Trp Phe Arg Asp Gln 
65                  70                  75                  80  


Tyr Pro Tyr Arg Asp Glu Asp Tyr Asn Val Thr Trp Phe Leu Ser Trp 
                85                  90                  95      


Ser Pro Cys Pro Thr Cys Ala Glu Glu Val Val Glu Phe Leu Glu Glu 
            100                 105                 110         


Tyr Arg Asn Leu Thr Leu Ser Ile Phe Thr Ser Arg Leu Tyr Tyr Phe 
        115                 120                 125             


Trp His Pro Asn Tyr Gln Glu Gly Leu Cys Lys Leu Trp Asp Ala Gly 
    130                 135                 140                 


Val Gln Leu Asp Ile Met Ser Cys Asp Glu Phe Glu Tyr Cys Trp Asp 
145                 150                 155                 160 


Asn Phe Val Tyr His Lys Gly Met Arg Phe Gln Arg Arg Asn Leu Leu 
                165                 170                 175     


Lys Asp Tyr Asp Phe Leu Ala Ala Lys Leu Gln Glu Ile Leu Ser Pro 
            180                 185                 190         


Gly Gln Gln Arg Lys Arg Asp Trp Pro Phe Pro Pro Arg Pro Gly Ala 
        195                 200                 205             


Gln Val Asp Pro Arg Ser Trp Val Gln Glu Val Thr Glu Pro Gly Ile 
    210                 215                 220                 


Asn Thr Arg Arg His Pro Leu His Leu Leu Val Ser Phe Leu Leu Pro 
225                 230                 235                 240 


Arg Pro Thr Met Asn Pro Leu Gln Glu Asp Ile Phe Tyr Arg Gln Phe 
                245                 250                 255     


Gly Asn Gln His Arg Val Pro Lys Pro Tyr Tyr Tyr Arg Arg Lys Thr 
            260                 265                 270         


Tyr Leu Cys Tyr Gln Leu Lys Leu Pro Glu Gly Thr Leu Ile Asp Lys 
        275                 280                 285             


Asp Cys Leu Arg Asn Lys Lys Lys Arg His Ala Glu Ile Cys Phe Ile 
    290                 295                 300                 


Asp Lys Ile Lys Ser Leu Thr Arg Asp Thr Ser Gln Arg Phe Glu Ile 
305                 310                 315                 320 


Ile Cys Tyr Ile Thr Trp Ser Pro Cys Pro Phe Cys Ala Glu Glu Leu 
                325                 330                 335     


Val Ala Phe Val Lys Asp Asn Pro His Leu Ser Leu Arg Ile Phe Ala 
            340                 345                 350         


Ser Arg Leu Tyr Val His Trp Arg Trp Lys Tyr Gln Gln Gly Leu Arg 
        355                 360                 365             


His Leu His Ala Ser Gly Ile Pro Val Ala Val Met Ser Leu Pro Glu 
    370                 375                 380                 


Phe Glu Asp Cys Trp Arg Asn Phe Val Asp His Gln Asp Arg Leu Phe 
385                 390                 395                 400 


Gln Pro Trp Arg Asn Leu Asp Gln Tyr Ser Glu Ser Ile Lys Arg Arg 
                405                 410                 415     


Leu Gly Lys Ile Leu Thr Pro Leu Asn Asp Leu Arg Asn Asp Phe Arg 
            420                 425                 430         


Asn Leu Lys Leu Glu 
        435         


<210>  73
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.3; 
       tr|A0A3Q0DM17|A0A3Q0DM17_TARSY polypeptide sequence

<400>  73

Met Pro Met Lys Arg Met Tyr Ser Asn Ile Tyr Phe Asp His Phe Asn 
1               5                   10                  15      


Asn Gln Arg Leu Leu Ser Gly Gln Asn Ala Pro Trp Leu Cys Phe Lys 
            20                  25                  30          


Val Glu Arg Val Glu Asn Cys Met Leu Val Pro Leu Glu Thr Gly Val 
        35                  40                  45              


Phe Gly Asn Gln Val Ser Gly Cys Cys Gly Lys Thr Glu Arg Pro Val 
    50                  55                  60                  


Glu Pro Thr Ser Leu Thr Arg Ser Val Leu Val Ser Pro Asn Pro Gly 
65                  70                  75                  80  


Thr Glu Leu Arg Ala Gln Gln Pro Ser Arg Lys Gly His Leu Gly Lys 
                85                  90                  95      


Leu Gly Cys Val Glu Tyr Pro Ser Pro Gly Leu Ala Leu Val Met Leu 
            100                 105                 110         


Gly Tyr Gly Ala Ser Thr Tyr Cys Pro Asp Ser Ser Met Tyr Cys Pro 
        115                 120                 125             


Glu Thr Cys His His Pro Glu Met Cys Phe Leu Tyr Trp Phe Glu Lys 
    130                 135                 140                 


Thr Leu Ser His Glu Glu Gln Tyr Gln Ile Thr Trp Tyr Val Ser Trp 
145                 150                 155                 160 


Ser Pro Cys Val Asn Cys Ala Glu Glu Val Ala Glu Phe Leu Ser Val 
                165                 170                 175     


His Pro Lys Val Asn Leu Thr Ile Tyr Ala Ala Arg Leu Tyr Cys Tyr 
            180                 185                 190         


Gln Lys Leu Asn His Arg Gln Gly Leu Arg Arg Leu Cys Lys Glu Gly 
        195                 200                 205             


Ala Cys Val Lys Ile Met Asn Tyr Glu Glu Phe Asp His Cys Trp Glu 
    210                 215                 220                 


Asn Phe Val Tyr Asn Asn Tyr Lys Ser Phe Lys Pro Trp Val Lys Leu 
225                 230                 235                 240 


Gln Asp Asn Tyr Glu Leu Leu Ala Thr Glu Leu Asp Lys Ile Leu Arg 
                245                 250                 255     


Ile Pro Met Glu Arg Met Pro Gln Lys Lys Phe Arg Phe His Phe Gln 
            260                 265                 270         


Asn Leu Ile Ala Lys Asp Arg Asn Thr Thr Trp Leu Cys Phe Glu Val 
        275                 280                 285             


Lys Asn Val Arg Lys Lys His Pro Pro Asp Leu Leu Glu Arg Gly Ile 
    290                 295                 300                 


Phe Gln Asn Gln Val Thr Pro Arg Ile Asn Cys His Ala Glu Met Cys 
305                 310                 315                 320 


Phe Leu Ser Trp Phe Leu Glu Asn Met Leu Leu His Gly Lys Arg Tyr 
                325                 330                 335     


Gln Val Thr Trp Tyr Ile Ser Trp Ser Pro Cys Ser Ile Cys Ala Glu 
            340                 345                 350         


Glu Val Ala Glu Phe Leu Ser Ala His Pro Lys Val Ser Leu Thr Ile 
        355                 360                 365             


Tyr Ala Ala Arg Leu Tyr Tyr Phe Trp Val Pro Gly Tyr Arg Gln Gly 
    370                 375                 380                 


Leu Arg Arg Leu Val Glu Glu Gly Ala Arg Val Glu Ile Met Asn Tyr 
385                 390                 395                 400 


Glu Glu Phe Asp Tyr Cys Trp Glu Asn Phe Val Ser Ile Asn Asn Glu 
                405                 410                 415     


Pro Phe Gln Pro Trp Glu Gly Leu His Glu Lys Tyr Gly Tyr Leu Val 
            420                 425                 430         


Thr Lys Leu Asn Asn Ile Leu Gly 
        435                 440 


<210>  74
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.4; 
       tr|A0A3Q0DNJ5|A0A3Q0DNJ5_TARSY polypeptide sequence

<400>  74

Met Glu Asp Asn Pro Glu Pro Arg Pro Arg Gln Gln Met Asp Gln Asp 
1               5                   10                  15      


Thr Phe Ile Phe Asn Phe Asn Asn Asp Pro Ser Val Arg Gly Arg His 
            20                  25                  30          


Gln Thr Phe Leu Cys Tyr Glu Val Glu His Leu Asp Asp Asp Thr Trp 
        35                  40                  45              


Val Pro Gln Asp Lys Tyr Leu Gly Phe Leu His Asn Gln Pro Gln Ser 
    50                  55                  60                  


Arg Ser Asn Ala Tyr Cys Ala Tyr His Ala Glu Leu Cys Phe Leu Glu 
65                  70                  75                  80  


Leu Val Ser Ser Trp Gln Leu Asp Pro Ala Gln Arg Tyr Arg Val Thr 
                85                  90                  95      


Cys Phe Ile Ser Trp Ser Pro Cys Ser Ser Cys Ala Gln Glu Val Ala 
            100                 105                 110         


Ala Phe Leu Lys Lys Asn Arg His Val Thr Leu Arg Ile Leu Ala Ala 
        115                 120                 125             


Arg Ile Tyr Asp Tyr Tyr Gln Gly Tyr Glu Asp Gly Leu Arg Thr Leu 
    130                 135                 140                 


Gln Gly Val Gly Val Asp Ile Thr Val Met Thr Ser Ala Glu Phe Gly 
145                 150                 155                 160 


His Cys Trp Asn Thr Phe Val Asp His Gln Gly Ser Pro Phe Gln Pro 
                165                 170                 175     


Trp Glu Gly Leu Asp Gln His Ser Gln Val Ile Trp Gln Arg Met Gln 
            180                 185                 190         


Asp Ile Leu Gln Val Ile Pro Ala Lys Tyr Leu Met Glu Lys Val Lys 
        195                 200                 205             


Tyr Thr Val Thr Val Asp Ile Leu Phe Lys Gly Arg Val Pro Gly Pro 
    210                 215                 220                 


Arg Tyr Leu Met Asp Gln Asn Thr Phe Thr Arg Asn Phe Ile Asn Asn 
225                 230                 235                 240 


Leu Ser Val Ser Gly Arg Arg Gln Thr Leu Leu Cys Tyr Glu Val Glu 
                245                 250                 255     


Arg Leu Gly Gly Asp Ile Trp Val Pro Leu Asp Gln Leu Arg Gly Phe 
            260                 265                 270         


Leu Leu Ser Gln Ala Arg Asp Val Leu Asn Tyr Tyr Gln Gly Arg His 
        275                 280                 285             


Ala Glu Pro Cys Phe Leu Asp Leu Val Ser Ser Trp Gln Leu Asp Pro 
    290                 295                 300                 


Ala Gln His Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys Thr 
305                 310                 315                 320 


Ser Cys Ala Gln Ala Val Ala Ala Phe Leu Arg Glu Asn Arg His Val 
                325                 330                 335     


Thr Leu Arg Ile Leu Ala Ala Arg Ile Tyr Asp Tyr His Gln Gly Tyr 
            340                 345                 350         


Glu Glu Gly Leu Arg Thr Leu Gln Arg Thr Gly Ala His Ile Asp Ile 
        355                 360                 365             


Met Thr Phe Lys Glu Phe Gly His Cys Trp Asn Thr Phe Val Asn His 
    370                 375                 380                 


Lys Gly Ser Pro Phe Lys Ser Trp Thr Gly Leu Asp Gln His Ser Gln 
385                 390                 395                 400 


Ala Leu Arg Lys Arg Leu Gln Asp Ile Leu His Thr Met Ala Ser Ser 
                405                 410                 415     


Leu Trp Asp Gln Ser Glu Pro Lys Lys Pro Ile Pro Ser Gln Glu Val 
            420                 425                 430         


Thr Leu Pro Glu Ser Ile Pro Pro Ser His Gly Asn Arg Phe Arg Leu 
        435                 440                 445             


Val Lys Arg Pro Ser 
    450             


<210>  75
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.5; 
       tr|G5AYU5|G5AYU5_HETGA polypeptide sequence

<400>  75

Phe Cys Phe Leu Ser Cys Val His Arg Lys Pro Ile Glu Arg Ile Tyr 
1               5                   10                  15      


Lys Lys Ala Phe Arg Phe Tyr Phe Arg Asn Leu Arg Cys Ala Tyr Gly 
            20                  25                  30          


Arg Asn Lys Thr Phe Leu Cys Tyr Glu Val Lys Arg Glu Arg Asp Asn 
        35                  40                  45              


Lys Val Leu His Lys Gly Val Val Leu Asn Gln Val Glu Pro Tyr Met 
    50                  55                  60                  


Pro Leu His Ala Glu Leu Arg Phe Leu Ser Trp Phe His Asp Thr Leu 
65                  70                  75                  80  


Leu Cys Pro Leu Gly Ser Tyr Gln Val Thr Leu Tyr Val Ser Trp Ser 
                85                  90                  95      


Pro Cys Ser Glu Cys Ala Glu Glu Leu Thr Thr Phe Leu Ala Gly His 
            100                 105                 110         


Arg Asn Val Thr Met Thr Ile Tyr Val Ala Gln Leu Tyr Tyr Cys Asn 
        115                 120                 125             


Trp Lys Ser Pro Asn Arg Glu Gly Leu Lys Ile Leu Ile Ala Glu Asp 
    130                 135                 140                 


Ala Arg Leu Arg Val Met Phe Tyr Asp Glu Phe Leu Tyr Cys Trp Arg 
145                 150                 155                 160 


Asn Phe Val Lys Asn Asp Tyr Asn Asn Phe Asp Pro Trp Ser Leu Leu 
                165                 170                 175     


Asp Glu Asn Ser Arg Tyr His Asn Arg Ile Leu Gln Asn Ile Leu Lys 
            180                 185                 190         


Gly Trp Gly Arg Pro His Arg Val Gly Pro Glu Gly Glu Gln Thr Ala 
        195                 200                 205             


Thr Pro Gly Gly Ser Gly Gly His Cys Ile Ser Val Phe Ser Leu Leu 
    210                 215                 220                 


Arg Arg Arg Glu Met Thr Leu Lys Glu Glu Thr Phe Arg Val Gln Phe 
225                 230                 235                 240 


Asn Asn Ala Tyr Lys Ala Pro Lys Pro Tyr Arg Arg Arg Val Thr Tyr 
                245                 250                 255     


Leu Cys Tyr Gln Leu Gln Glu Ala Asn Gly Asp Pro Leu Thr Lys Gly 
            260                 265                 270         


Cys Leu Arg Thr Lys Lys Gly Tyr His Ala Glu Ser Arg Phe Ile Lys 
        275                 280                 285             


Arg Ile Cys Ser Met Asp Leu Gly Gln Asp Gln Ser Tyr Gln Val Thr 
    290                 295                 300                 


Cys Phe Leu Thr Trp Ser Pro Cys Pro His Cys Ala Gln Glu Leu Val 
305                 310                 315                 320 


Ser Phe Lys Arg Ala His Pro His Leu Arg Leu Gln Ile Phe Thr Ala 
                325                 330                 335     


Arg Leu Phe Phe His Trp Lys Arg Ser Tyr Gln Glu Gly Leu Gln Arg 
            340                 345                 350         


Leu Cys Arg Ala Gln Val Pro Val Ala Val Met Gly His Pro Glu Phe 
        355                 360                 365             


Ala Tyr Cys Trp Asp Asn Phe Val Asp His Gln Pro Gly Pro Phe Glu 
    370                 375                 380                 


Pro Pro Trp Ala Lys Leu Glu Tyr Tyr Ser Ser Cys Leu Lys Arg Arg 
385                 390                 395                 400 


Leu Gln Gln Ile Leu Arg Ser Trp Gly Val Asp Asp Leu Thr Asn Asp 
                405                 410                 415     


Phe Arg Asn Leu Gln Leu Gly Pro 
            420                 


<210>  76
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.6; 
       tr|A0A2Y9QMV5|A0A2Y9QMV5_TRIMA polypeptide sequence

<400>  76

Met Leu Ser Ser Pro Gln Thr Pro Gly Thr Arg Lys Pro Met Lys Thr 
1               5                   10                  15      


Leu Ala Pro Asp Glu Phe Ser Phe Asn Phe Glu Asn Leu Arg Leu Ala 
            20                  25                  30          


His Gly Arg Asn Thr Thr Phe Leu Cys Phe Gln Val Glu Thr Lys Ala 
        35                  40                  45              


Pro Pro Ser Leu Asn Ser Pro Asp Ser Gly Ile Phe Gln Asn Gln Asp 
    50                  55                  60                  


His Cys Pro Ser His His His Ala Glu Met Val Phe Leu Thr Trp Phe 
65                  70                  75                  80  


Gln Lys Arg Leu Ser Pro Ala Gln His Tyr Glu Val Thr Trp Tyr Met 
                85                  90                  95      


Ser Trp Ser Pro Cys Ser Arg Cys Ala Val Gln Val Ala Lys Phe Leu 
            100                 105                 110         


Lys Ser Asn Ser Thr Val Asn Leu Ser Ile Phe Val Ala Arg Leu Tyr 
        115                 120                 125             


Tyr Pro Arg Glu Leu Glu Thr Lys Asp Gly Leu His Ser Leu Trp Gln 
    130                 135                 140                 


Ala Gly Ala Gln Val Gln Ile Met Phe Phe Gln Asp Phe Lys Tyr Cys 
145                 150                 155                 160 


Trp Glu Asn Phe Val Asn Asn Glu Gly Lys Pro Phe Gln Pro Trp Lys 
                165                 170                 175     


Asn Leu Asp Glu Asn Ser Lys Asp Trp Asp Thr Glu Leu Lys Asp Ile 
            180                 185                 190         


His Arg Asn Thr Thr Asp Leu Leu Thr Glu Glu Met Phe Tyr Ser Gln 
        195                 200                 205             


Phe Tyr Asn Arg Glu Lys Lys Ser Ser Ile Pro Arg Lys Thr Tyr Leu 
    210                 215                 220                 


Cys Tyr Gln Leu Asn Glu Pro Gln Pro Val Lys Arg Cys Leu His Tyr 
225                 230                 235                 240 


Lys Lys Gly Tyr His Ala Val Thr Arg Phe Ile Asp Gly Ile Val Ser 
                245                 250                 255     


Met Asn Leu Asp Pro Ala Arg Ser Tyr Asp Ile Thr Cys Tyr Phe Thr 
            260                 265                 270         


Trp Ser Pro Cys Asn Arg Tyr Ala Arg Lys Leu Val Ser Phe Ile Glu 
        275                 280                 285             


Asp Tyr Pro Asn Leu Arg Leu Lys Val Tyr Thr Ser Arg Leu Tyr Phe 
    290                 295                 300                 


His Trp Cys Trp Thr Asn Met Gln Gly Leu Gln His Leu Gln Asn Ser 
305                 310                 315                 320 


Arg Val Thr Val Ala Val Met Thr Phe Arg Asp Phe Glu Tyr Cys Trp 
                325                 330                 335     


Lys Asn Phe Val Asp Asn Gln Gly Lys Pro Phe Glu Pro Trp Glu Lys 
            340                 345                 350         


Leu Asp Leu Tyr Ser Gln Ser Thr Glu Arg Arg Leu Arg Arg Ile Leu 
        355                 360                 365             


Lys Pro Leu Thr Pro Asp Val Leu Asn Glu Asp Phe Gly Asn Leu His 
    370                 375                 380                 


Leu 
385 


<210>  77
<211>  381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.7; 
       tr|H0XHI0|H0XHI0_OTOGA polypeptide sequence

<400>  77

Leu Ser Cys Ala Phe Arg Asp Pro Met Asn Arg Met Tyr Pro Lys Thr 
1               5                   10                  15      


Phe Cys Gln Asn Phe Glu Lys Glu Pro Cys Pro Ser Asn Gln Asn Ser 
            20                  25                  30          


Ser Trp Leu Cys Phe Glu Val Glu Thr Lys Asn Ser Ala Val Phe Phe 
        35                  40                  45              


His Arg Gly Val Phe Arg Asn Gln Pro Ala Pro Pro Pro Arg Ala Pro 
    50                  55                  60                  


Thr Ser Val Leu Leu Ser Gln Gly Pro Val Lys Thr Pro Cys His Ala 
65                  70                  75                  80  


Glu Glu Cys Phe Leu Thr Trp Ile Gln Gly Val Leu Pro Pro Asp His 
                85                  90                  95      


His Tyr His Val Thr Trp Tyr Val Ser Arg Gly Pro Cys Ala Asn Cys 
            100                 105                 110         


Ala Asn Leu Ile Val His Phe Leu Ala Met His Arg Arg Val Thr Leu 
        115                 120                 125             


Thr Ile Phe Ala Ala His Leu Asn Phe Phe Trp Glu Ser Asp Phe Gln 
    130                 135                 140                 


Gln Gly Leu Leu Arg Met Asp Gln Glu Gly Val Gln Leu His Ile Met 
145                 150                 155                 160 


Gly Tyr Glu Glu Phe Glu Tyr Cys Trp Asp Asn Phe Val Tyr Asn Gln 
                165                 170                 175     


Arg Lys Gln Phe Val Pro Trp Asn Gly Leu Asn Glu Asn Tyr Glu Phe 
            180                 185                 190         


Met Val Ser Thr Leu Glu Asp Ile Leu Arg Ser Pro Leu Asp Arg Ile 
        195                 200                 205             


Arg Gln Lys Asp Phe Ser Ile His Phe Arg Asn Ser Leu Trp Leu Asp 
    210                 215                 220                 


Asp Lys Ser Thr Trp Leu Cys Phe Glu Val Lys Arg Thr Lys Ser Pro 
225                 230                 235                 240 


Val Pro Leu Tyr Arg Gly Val Phe Arg Asn Gln Ser Pro Pro Lys Thr 
                245                 250                 255     


Pro Cys His Ala Glu Val Arg Phe Phe Thr Trp Leu Gln Asp Leu Pro 
            260                 265                 270         


Pro Asp Phe Cys Cys Gln Phe Thr Trp Tyr Leu Ser Trp Ser Pro Cys 
        275                 280                 285             


Ala Asp Cys Ala Asp Leu Val Ala Asn Phe Leu Ala Lys His Arg Asn 
    290                 295                 300                 


Val Ser Leu Thr Ile Phe Val Ala Arg Leu Tyr Tyr Tyr Arg Asp Pro 
305                 310                 315                 320 


Glu Met His Arg Gly Leu Arg Arg Met Tyr Gln Glu Gly Ala Asn Val 
                325                 330                 335     


Asp Ile Met Ser Val Ile Glu Phe Glu Tyr Cys Trp Asp Asn Phe Val 
            340                 345                 350         


Tyr Asn Gln Gly Lys Gln Phe Val Pro Trp Asn Gly Leu Asn Glu Asn 
        355                 360                 365             


Tyr Glu Phe Leu Val Pro Arg Leu Gln Glu Ile Leu Glu 
    370                 375                 380     


<210>  78
<211>  337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.8; 
       tr|A0A3M0K4Y7|A0A3M0K4Y7_HIRRU polypeptide sequence

<400>  78

Met Tyr Ile Ser Lys Lys Ala Leu Arg Arg His Phe Asp Pro Arg Val 
1               5                   10                  15      


Tyr Pro Arg Glu Thr Tyr Leu Leu Cys Glu Leu Gln Trp Glu Gly Ser 
            20                  25                  30          


Arg Arg Val Trp Ile His Trp Ile Arg Asn Val Pro Asp His His Ala 
        35                  40                  45              


Glu Glu Tyr Phe Leu Glu Glu Val Phe Glu Pro Arg Asn Tyr Gly Phe 
    50                  55                  60                  


Cys Asn Ile Thr Leu Tyr Leu Ser Trp Ser Pro Cys Cys Thr Cys Cys 
65                  70                  75                  80  


Ser Lys Ile Arg Asp Phe Leu Lys Arg Asn Pro Asn Val Lys Ile Asp 
                85                  90                  95      


Ile Arg Val Ala Arg Leu Ile Tyr Pro Asp Tyr Ala Glu Thr Arg Ser 
            100                 105                 110         


Ser Leu Arg Glu Leu Asn Gly Leu Gln Arg Val Ser Ile Gln Val Met 
        115                 120                 125             


Glu Ala Ala Gly Leu Ser Cys Ile Glu Ser Lys Asn His Arg Ile Ser 
    130                 135                 140                 


Gln Val Glu Arg Asp Pro Lys Gly Ser Ser Ser Pro Thr Leu Phe Thr 
145                 150                 155                 160 


Leu Gln Asp His Leu Lys Leu Ser Asn Met Thr Glu Ser Val Ile Gln 
                165                 170                 175     


Asp Ser Val Ser Ile Gln Ile Cys Tyr Gln Met Arg Ile Leu Gly Phe 
            180                 185                 190         


Gln Cys His Ile Arg Trp Lys Leu Gln Pro Glu Asp Phe Gln Arg Asn 
        195                 200                 205             


Tyr Ser Pro Asn Gln Ile Gly Arg Val Val Tyr Leu Leu Tyr Glu Val 
    210                 215                 220                 


Arg Trp Arg Arg Gly Ser Ile Trp Arg Asn Trp Cys Ser Asn Asn Pro 
225                 230                 235                 240 


Glu Gln His Ala Glu Val Asn Phe Leu Glu Asn His Phe His His Arg 
                245                 250                 255     


Pro Gln Thr Pro Cys Ser Ile Thr Trp Phe Leu Ser Thr Ser Pro Cys 
            260                 265                 270         


Gly Lys Cys Ser Arg Arg Ile Leu Glu Phe Leu Lys Ser Gln Pro Asn 
        275                 280                 285             


Val Thr Leu Glu Ile Tyr Ala Ala Lys Leu Phe Arg His His Asp Ile 
    290                 295                 300                 


Arg Asn Arg Gln Gly Leu Arg Asn Leu Met Met Asn Gly Val Thr Ile 
305                 310                 315                 320 


Tyr Ile Met Asn Leu Glu Gly Asn Pro Ala Ser Leu Cys Leu Ser Val 
                325                 330                 335     


Asp 
    


<210>  79
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.9; 
       tr|A0A3P4LUZ8|A0A3P4LUZ8_GULGU polypeptide sequence

<400>  79

Met Ser Phe Glu Asp Tyr Glu Tyr Cys Trp Glu Thr Phe Val Asp His 
1               5                   10                  15      


Lys Gly Met Tyr Phe Gln Ser Trp Asp Leu Leu Arg Asp Asn Asp Leu 
            20                  25                  30          


Leu Ala Ala Glu Leu Lys Asn Ile Leu Arg Ser Thr Met Asn Pro Leu 
        35                  40                  45              


Arg Gln Glu Ile Phe Tyr His Gln Phe Gly Asn Gln Pro Arg Ala Pro 
    50                  55                  60                  


Arg Pro Tyr His Arg Arg Lys Thr Tyr Leu Cys Tyr Gln Leu Gln Pro 
65                  70                  75                  80  


His Glu Gly Pro Ile Thr Ala Arg Val Cys Leu Gln Asn Lys Lys Lys 
                85                  90                  95      


Arg His Ala Glu Ile Arg Phe Ile Asp Asn Ile Arg Ala Leu Arg Leu 
            100                 105                 110         


Asp Arg Ser Gln Thr Phe Glu Ile Thr Cys Tyr Leu Thr Trp Ser Pro 
        115                 120                 125             


Cys Pro Thr Cys Ala Lys Ala Leu Ala Val Phe Val Gln Asp His Pro 
    130                 135                 140                 


His Ile Ser Leu Arg Leu Phe Ala Ser Arg Leu Phe Ile His Trp Cys 
145                 150                 155                 160 


Trp Lys Tyr Gln Glu Gly Leu Arg Leu Leu His Arg Ser Arg Ile Pro 
                165                 170                 175     


Val Ala Val Met Arg Leu Gln Glu Phe Glu Asp Cys Trp Arg Asn Phe 
            180                 185                 190         


Val Asp Asn Gln Asp Glu Pro Phe Gln Pro Trp Asn Lys Leu Glu Gln 
        195                 200                 205             


Tyr Ser Glu Ser Ile Thr Arg Arg Leu Arg Arg Ile Leu Gly His Pro 
    210                 215                 220                 


Gln Asn Asn Leu Glu Asn Asp Phe Arg Asn Leu His Ile 
225                 230                 235         


<210>  80
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.10; 
       tr|G5BPM8|G5BPM8_HETGA polypeptide sequence

<400>  80

Arg Arg Arg Ile Glu Pro Trp Gln Phe Glu Ala Ser Phe Asp Pro Arg 
1               5                   10                  15      


Gln Leu Arg Arg Glu Thr Cys Leu Leu Ser Glu Val Arg Trp Gly Thr 
            20                  25                  30          


Ser Pro Arg Ala Trp Arg Gly Cys Ser Leu Asn Thr Ala Arg His Ala 
        35                  40                  45              


Glu Val Ser Phe Met Asp Arg Leu Thr Ser Glu Gly Arg Leu Arg Gly 
    50                  55                  60                  


Pro Val Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly 
65                  70                  75                  80  


Ala Cys Ala Gln Ala Ile Gly Glu Phe Leu Arg Gln His Pro Asn Val 
                85                  90                  95      


Ser Leu Val Ile Tyr Ile Ala Arg Leu Phe Trp His Val Asp Glu Gln 
            100                 105                 110         


Asn Arg Gln Gly Leu Arg Asp Leu Val Thr Arg Gly Val Arg Met Gln 
        115                 120                 125             


Val Met Ser Asp Pro Glu Phe Ala His Cys Trp Arg Asn Phe Val Asn 
    130                 135                 140                 


Tyr Ser Pro Gly Gln Glu Ala Arg Trp Pro Gln Val Pro Pro Val Trp 
145                 150                 155                 160 


Thr Trp Leu Tyr Ser Leu Glu Leu His Cys Ile Leu Leu Asn Leu Pro 
                165                 170                 175     


Pro Cys Leu Lys Ile Ser Arg Arg His His Asn Gln Leu Thr Phe Phe 
            180                 185                 190         


Gln Leu Ile Leu Gln Asn Cys His Tyr Gln Ala Ile Pro Ser Pro Val 
        195                 200                 205             


Leu Leu Ala Ser Gly Leu Ile His Pro Phe Val Thr Trp 
    210                 215                 220     


<210>  81
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.11; 
       tr|H2M862|H2M862_ORYLA polypeptide sequence

<400>  81

Met Ile Thr Lys Leu Asp Ser Val Leu Leu Pro Lys Lys Lys Phe Ile 
1               5                   10                  15      


Tyr His Tyr Lys Asn Met Arg Trp Ala Arg Gly Arg His Glu Thr Tyr 
            20                  25                  30          


Leu Cys Phe Val Val Lys Arg Arg Val Gly Pro Glu Ser Leu Ser Phe 
        35                  40                  45              


Asp Phe Gly His Leu Arg Asn Arg Asn Gly Cys His Val Glu Leu Leu 
    50                  55                  60                  


Phe Leu Arg His Leu Ser Ala Leu Cys Pro Gly Leu Trp Gly Tyr Gly 
65                  70                  75                  80  


Ala Thr Gly Gln Gly Arg Val Ser Tyr Ser Ile Thr Trp Phe Cys Ser 
                85                  90                  95      


Trp Ser Pro Cys Ala Asn Cys Ser Phe Arg Leu Ala Gln Phe Leu Ser 
            100                 105                 110         


Gln Thr Pro Asn Leu Arg Leu Arg Ile Phe Val Ser Arg Leu Tyr Phe 
        115                 120                 125             


Cys Asp Leu Glu Asp Ser Arg Glu Arg Glu Gly Leu Arg Met Leu Lys 
    130                 135                 140                 


Lys Val Gly Val His Ile Thr Val Met Ser Tyr Lys Asp Tyr Phe Tyr 
145                 150                 155                 160 


Cys Trp Gln Thr Phe Val Ala Arg Lys Gln Ser Lys Phe Lys Pro Trp 
                165                 170                 175     


Asp Gly Leu His Gln Asn Ser Val Arg Leu Ser Arg Lys Leu Asn Arg 
            180                 185                 190         


Ile Leu Gln Pro Cys Glu Thr Glu Asp Phe Arg Asp Ala Phe Lys Leu 
        195                 200                 205             


Leu Gly Leu 
    210     


<210>  82
<211>  353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.12; 
       tr|H0Y0C6|H0Y0C6_OTOGA polypeptide sequence

<400>  82

Met Tyr Leu Lys Thr Phe Tyr Arg His Phe Asn Asn Arg Pro Tyr Leu 
1               5                   10                  15      


Ser Arg Arg Asn Asp Thr Trp Leu Cys Phe Glu Val Lys Thr Thr Ser 
            20                  25                  30          


Ser Asn Ser Pro Gly Ser Phe Tyr Ser Gly Val Phe Arg Asn Gln Gly 
        35                  40                  45              


Pro Arg Tyr Cys Pro Trp His Thr Glu Leu Cys Phe Leu Thr Trp Val 
    50                  55                  60                  


Arg Pro Ile Val Ser His His His Phe Tyr Gln Ile Thr Trp Tyr Met 
65                  70                  75                  80  


Ser Trp Ser Pro Cys Ala Asn Cys Ala Trp Gln Val Ala Thr Phe Leu 
                85                  90                  95      


Ala Thr His Glu Asn Val Ser Leu Thr Asn Tyr Thr Val Arg Ile Tyr 
            100                 105                 110         


Tyr Phe Trp Arg Gln Asp Tyr Arg Gln Gly Leu Leu Arg Met Ile Glu 
        115                 120                 125             


Glu Gly Thr Gln Val Tyr Val Met Ser Ser Lys Glu Phe Gln His Cys 
    130                 135                 140                 


Trp Glu Asn Phe Val Asp His Trp Gly Thr Arg Trp Val Thr Cys Trp 
145                 150                 155                 160 


Asn Arg Leu Lys Lys Asn Tyr Glu Phe Leu Val Thr Arg Leu Ser Glu 
                165                 170                 175     


Ile Leu Ser Asp Pro Lys Glu Arg Ile Ser Pro Asn Thr Phe Tyr Asn 
            180                 185                 190         


Gln Phe Asn Asn Thr Pro Val Pro Arg Gly Arg Lys Asp Thr Trp Leu 
        195                 200                 205             


Cys Phe Glu Val Lys Glu Lys Asn Ser Asn Ser Pro Gly Ser Phe His 
    210                 215                 220                 


Arg Gly Val Phe Gln Asn Gln Val Phe Ser Gly Thr Ser Ser His Ala 
225                 230                 235                 240 


Arg Arg Cys Pro Pro Asp His His Tyr Glu Val Thr Trp Tyr Thr Ser 
                245                 250                 255     


Trp Ser Pro Cys Ala His Cys Ala Trp His Val Val Asn Phe Leu Thr 
            260                 265                 270         


Ser Asn Pro Asn Val Ser Leu Thr Ile Phe Ala Ala Arg Leu Tyr Tyr 
        275                 280                 285             


Ile Tyr Arg Pro Glu Ile Gln Gln Gly Leu Arg Arg Val Phe Gln Glu 
    290                 295                 300                 


Gly Ala Lys Val His Ile Met Ser Leu Lys Glu Phe Lys Tyr Cys Trp 
305                 310                 315                 320 


Ala Lys Leu Val Tyr Asn Ser Gly Met Arg Phe Met Pro Trp Tyr Gln 
                325                 330                 335     


Phe Asn Phe Asn Phe Leu Phe Pro Asn Thr Thr Leu Lys Gly Asp Leu 
            340                 345                 350         


His 
    


<210>  83
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.13; 
       tr|A0A3Q2Z5X6|A0A3Q2Z5X6_HIPCM polypeptide sequence

<400>  83

Met Asp Val His Phe Met Asn Phe Ile Tyr His Tyr Lys Asn Met Arg 
1               5                   10                  15      


Trp Ala Lys Gly Arg Asn Glu Thr Tyr Leu Cys Phe Val Val Lys Arg 
            20                  25                  30          


Arg Val Gly Pro Asn Ser Leu Thr Phe Asp Phe Gly His Leu Arg Asn 
        35                  40                  45              


Arg Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr Leu Gly Arg 
    50                  55                  60                  


Arg Leu Ser Tyr Ser Ile Thr Trp Phe Cys Ser Trp Ser Pro Cys Ala 
65                  70                  75                  80  


Asn Cys Ser Ala Ala Leu Ser Gln Phe Leu Ser Arg Met Pro Asn Leu 
                85                  90                  95      


Arg Leu Arg Ile Phe Val Ala Arg Leu Tyr Phe Cys Asp Met Glu Asp 
            100                 105                 110         


Ser His Glu Arg Glu Gly Leu Arg Leu Leu Gln Lys Ala Gly Val Gln 
        115                 120                 125             


Val Thr Val Met Ser Tyr Lys Asp Tyr Tyr Tyr Cys Trp Gln Thr Phe 
    130                 135                 140                 


Val Asp Arg Lys Lys Ser His Phe Lys Ala Trp Glu Asp Leu His Gln 
145                 150                 155                 160 


Asn Ser Val Arg Leu Ser Arg Lys Leu Asn Arg Ile Leu Gln Pro Cys 
                165                 170                 175     


Glu Met Asp Leu Arg Asp Ala Phe Lys Leu Leu Gly Leu 
            180                 185                 


<210>  84
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.15; 
       tr|A0A2K6NY90|A0A2K6NY90_RHIRO polypeptide sequence

<400>  84

Met Asn Pro His Ile Arg Asn Pro Met Glu Ala Met Tyr Pro Gly Thr 
1               5                   10                  15      


Phe Tyr Phe His Phe Lys Asn Leu Trp Glu Ala Asp Asn Arg Asn Glu 
            20                  25                  30          


Ser Trp Leu Cys Phe Ala Val Glu Val Ile Lys His His Ser Thr Val 
        35                  40                  45              


Ser Trp Lys Arg Gly Val Phe Arg Asn Gln Val Asp Pro Glu Thr His 
    50                  55                  60                  


Cys His Ala Glu Lys Cys Phe Leu Ser Trp Phe Cys Asp Asn Thr Leu 
65                  70                  75                  80  


Ser Pro Lys Lys Asn Tyr Gln Val Thr Trp Tyr Thr Ser Trp Ser Pro 
                85                  90                  95      


Cys Pro Glu Cys Ala Arg Glu Val Ala Lys Phe Leu Ala Arg His Ser 
            100                 105                 110         


Asn Val Met Leu Thr Ile Tyr Thr Ala Arg Leu Tyr Tyr Ser Gln Tyr 
        115                 120                 125             


Pro Asn Tyr Gln Glu Gly Leu Arg Arg Leu Asn Glu Glu Gly Val Pro 
    130                 135                 140                 


Val Glu Ile Met Asp Tyr Glu Asp Phe Lys Tyr Cys Trp Glu Asn Phe 
145                 150                 155                 160 


Val Tyr Asn Gly Asp Glu Leu Phe Lys Pro Trp Lys Gly Leu Lys Tyr 
                165                 170                 175     


Asn Phe Leu Phe Leu Asp Ser Lys Leu Gln Glu Ile Leu Glu 
            180                 185                 190 


<210>  85
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.16; 
       tr|Q6ICH2|Q6ICH2_HUMAN polypeptide sequence

<400>  85

Met Asn Pro Gln Ile Arg Asn Pro Met Glu Arg Met Tyr Arg Asp Thr 
1               5                   10                  15      


Phe Tyr Asp Asn Phe Glu Asn Glu Pro Ile Leu Tyr Gly Arg Ser Tyr 
            20                  25                  30          


Thr Trp Leu Cys Tyr Glu Val Lys Ile Lys Arg Gly Arg Ser Asn Leu 
        35                  40                  45              


Leu Trp Asp Thr Gly Val Phe Arg Gly Pro Val Leu Pro Lys Arg Gln 
    50                  55                  60                  


Ser Asn His Arg Gln Glu Val Asp Pro Glu Thr His Cys His Ala Glu 
65                  70                  75                  80  


Arg Cys Phe Leu Ser Trp Phe Cys Asp Asp Ile Leu Ser Pro Asn Thr 
                85                  90                  95      


Asn Tyr Glu Val Thr Trp Tyr Thr Ser Trp Ser Pro Cys Pro Glu Cys 
            100                 105                 110         


Ala Gly Glu Val Ala Glu Phe Leu Ala Arg His Ser Asn Val Asn Leu 
        115                 120                 125             


Thr Ile Phe Thr Ala Arg Leu Cys Tyr Phe Trp Asp Thr Asp Tyr Gln 
    130                 135                 140                 


Glu Gly Leu Cys Ser Leu Ser Gln Glu Gly Ala Ser Val Lys Ile Met 
145                 150                 155                 160 


Gly Tyr Lys Asp Phe Val Ser Cys Trp Lys Asn Phe Val Tyr Ser Asp 
                165                 170                 175     


Asp Glu Pro Phe Lys Pro Trp Lys Gly Leu Gln Thr Asn Phe Arg Leu 
            180                 185                 190         


Leu Lys Arg Arg Leu Arg Glu Ile Leu Gln 
        195                 200         


<210>  86
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.17; 
       tr|G8GPV1|G8GPV1_CERNE polypeptide sequence

<400>  86

Met Asp Gly Ser Pro Ala Ser Arg Pro Gly His Val Met Asp Pro Gly 
1               5                   10                  15      


Thr Phe Thr Ser Asn Phe Asn Asn Lys Pro Trp Val Ser Gly Gln Arg 
            20                  25                  30          


Glu Thr Tyr Leu Cys Tyr Lys Val Glu Arg Ser His Asn Asp Thr Trp 
        35                  40                  45              


Val Leu Leu Asn Gln His Arg Gly Phe Leu Arg Asn Gln Ala Lys Asn 
    50                  55                  60                  


Arg Leu His Gly Asp Tyr Gly Cys His Ala Glu Leu Cys Phe Leu Gly 
65                  70                  75                  80  


Glu Val Pro Ser Trp Arg Leu Asp Pro Thr Gln Thr Tyr Arg Val Thr 
                85                  90                  95      


Trp Phe Ile Ser Trp Ser Pro Cys Phe Ser Gly Gly Cys Ala Glu Gln 
            100                 105                 110         


Val Arg Ala Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe 
        115                 120                 125             


Ala Ala Arg Ile Tyr Asp Tyr Asp Phe Leu Tyr Gln Glu Ala Leu Arg 
    130                 135                 140                 


Thr Leu Arg Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Glu Glu 
145                 150                 155                 160 


Phe Lys His Cys Trp Asp Thr Phe Val Asp His Gln Gly Arg Pro Phe 
                165                 170                 175     


Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg 
            180                 185                 190         


Leu Gln Ala Ile Leu Gln Asn Gln Gly Asn 
        195                 200         


<210>  87
<211>  181
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.18; 
       tr|Q1WBT6|Q1WBT6_SYMSY polypeptide sequence

<400>  87

Met Ala Leu Leu Thr Ala Lys Thr Phe Arg Leu Gln Phe Asn Asn Lys 
1               5                   10                  15      


Arg Arg Val Thr Lys Pro Tyr Tyr Pro Arg Lys Ala Leu Leu Cys Tyr 
            20                  25                  30          


Gln Leu Thr Pro Gln Asn Gly Ser Thr Pro Thr Arg Gly Tyr Phe Lys 
        35                  40                  45              


Asn Lys Lys Lys Arg His Ala Glu Ile Arg Phe Ile Asn Lys Ile Lys 
    50                  55                  60                  


Ser Met Gly Leu Asp Glu Thr Gln Cys Tyr Gln Val Thr Cys Tyr Leu 
65                  70                  75                  80  


Thr Trp Ser Pro Cys Pro Ser Cys Ala Trp Glu Leu Val Asp Phe Ile 
                85                  90                  95      


Lys Ala His Asp His Leu Asn Leu Gly Ile Phe Ala Ser Arg Leu Tyr 
            100                 105                 110         


Tyr His Trp Cys Arg His Gln Gln Glu Gly Leu Arg Leu Leu Cys Gly 
        115                 120                 125             


Ser Gln Val Pro Val Glu Val Met Gly Phe Pro Glu Phe Ala Asp Cys 
    130                 135                 140                 


Trp Glu Asn Phe Val Asp His Glu Glu Pro Leu Ser Phe Asn Pro Ser 
145                 150                 155                 160 


Glu Met Leu Glu Glu Leu Asp Lys Asn Ser Arg Ala Ile Lys Arg Arg 
                165                 170                 175     


Leu Glu Lys Ile Lys 
            180     


<210>  88
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.19; 
       tr|A0A3B4CS14|A0A3B4CS14_PYGNA polypeptide sequence

<400>  88

Met Asp Asn Thr Asn Arg Arg Lys Phe Ile Tyr His Tyr Lys Asn Val 
1               5                   10                  15      


Arg Trp Ala Arg Gly Arg His Glu Thr Tyr Leu Cys Phe Val Val Lys 
            20                  25                  30          


Lys Arg Asn Ser Pro Asp Ser Leu Ser Phe Asp Phe Gly His Leu Arg 
        35                  40                  45              


Asn Arg Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr Ile Glu 
    50                  55                  60                  


Val Leu Cys Pro Gly Leu Trp Gly Ser Gly Val Asp Gly Val Arg Val 
65                  70                  75                  80  


Ser Tyr Ala Val Thr Trp Phe Cys Ser Trp Ser Pro Cys Ser Asn Cys 
                85                  90                  95      


Ala Gln Arg Leu Thr Asn Phe Leu Ser Gln Thr Pro Asn Leu Arg Leu 
            100                 105                 110         


Arg Ile Phe Val Ala Arg Leu Tyr Phe Cys Asp Glu Glu Asp Ser Leu 
        115                 120                 125             


Glu Arg Glu Gly Leu Arg His Leu Gln Arg Ala Gly Val Gln Ile Thr 
    130                 135                 140                 


Val Met Thr Tyr Lys Asp Phe Phe Tyr Cys Trp Gln Thr Phe Val Ala 
145                 150                 155                 160 


Ser Arg Glu Arg Cys Phe Lys Ala Trp Glu Gly Leu Arg Gln Asn Ser 
                165                 170                 175     


Val Arg Leu Ser Arg Lys Leu Asn Arg Ile Leu Gln Val Phe Ile Ser 
            180                 185                 190         


Thr Pro Val Ile Ser Pro Leu Ile Thr Thr His Leu Gly Gln Ser Trp 
        195                 200                 205             


Ala Gly Gly 
    210     


<210>  89
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.20; 
       tr|A0A087XZI4|A0A087XZI4_POEFO polypeptide sequence

<400>  89

Arg Lys Val Ser Tyr Ser Val Thr Trp Phe Cys Ser Trp Ser Pro Cys 
1               5                   10                  15      


Ala Asn Cys Ser Ile Arg Leu Ala Gln Phe Leu His Gln Thr Pro Asn 
            20                  25                  30          


Leu Arg Leu Arg Ile Phe Val Ser Arg Leu Tyr Phe Cys Asp Leu Glu 
        35                  40                  45              


Asp Ser Arg Glu Arg Glu Gly Leu Arg Ile Leu Lys Lys Ala Gly Val 
    50                  55                  60                  


His Ile Thr Val Met Ser Tyr Lys Asp Tyr Phe Tyr Cys Trp Gln Thr 
65                  70                  75                  80  


Phe Val Ala Lys Ser Gln Ser Lys Phe Lys Pro Trp Asp Gly Leu His 
                85                  90                  95      


Gln Asn Tyr Ile Arg Leu Ser Arg Lys Leu Asn Arg Ile Leu Gln Pro 
            100                 105                 110         


Ala Leu Asp Ile Lys Lys Phe Ile Tyr His Tyr Lys Asn Leu Arg Trp 
        115                 120                 125             


Ala Arg Gly Arg Cys Glu Thr Tyr Leu Cys Phe Val Val Lys Lys Lys 
    130                 135                 140                 


Leu His Leu Phe Met Phe Val Ile Val Gly Arg Asn Arg Leu Phe Asp 
145                 150                 155                 160 


Leu Asn Val Thr Met Asn Asn Lys Ser Leu Tyr Leu Ile Pro Leu His 
                165                 170                 175     


Leu Gln Leu Leu Phe Leu Arg His Leu Gly Ala Leu Cys Pro Gly Leu 
            180                 185                 190         


Trp Gly Tyr Gly Val Thr Gly Glu Arg Lys Val Ser Tyr Ser Val Thr 
        195                 200                 205             


Trp Phe Cys Ser Trp Ser Pro Cys Ala Asn Cys Ser Ile Arg Leu Ala 
    210                 215                 220                 


Gln Phe Leu His Gln Thr Pro Asn Leu Arg Leu Arg Ile Phe Val Ser 
225                 230                 235                 240 


Arg Leu Tyr Phe Cys Asp Leu Glu Asp Ser Arg Glu Arg Glu Gly Leu 
                245                 250                 255     


Arg Ile Leu Lys Lys Ala Gly Val His Ile Thr Val Met Ser Tyr Lys 
            260                 265                 270         


Asp Tyr Phe Tyr Cys Trp Gln Thr Phe Val Ala Lys Ser Gln Ser Lys 
        275                 280                 285             


Phe Lys Pro Trp Asp Gly Leu His Gln Asn Tyr Ile Arg Leu Ser Arg 
    290                 295                 300                 


Lys Leu Asn Arg Ile Leu Gln Val Gln Phe Phe 
305                 310                 315 


<210>  90
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.21; 
       tr|A0A341AEK4|A0A341AEK4_9CETA polypeptide sequence

<400>  90

Met Ala Ser Asp Arg Gly Pro Ser Ala Gly Asp Ala Thr Ser Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro Trp Glu Phe Glu Val Ser Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Cys Lys Glu Thr Arg Leu Leu Tyr Glu Ile Lys Trp Gly Arg Ser Gln 
        35                  40                  45              


His Val Trp Arg His Ser Gly Lys Asn Thr Thr Asn His Val Glu Cys 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Ser Glu Arg Pro Phe His Arg Ser Val 
65                  70                  75                  80  


Ser Cys Cys Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu Cys 
                85                  90                  95      


Ser Lys Ala Ile Arg Glu Phe Leu Asn Gln His Pro Arg Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Val Ala Arg Leu Phe Gln His Met Asp Pro Gln Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile His Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Gly Pro Thr Glu Tyr Asp Tyr Cys Trp Arg Asn Phe Val Asn Tyr Pro 
145                 150                 155                 160 


Pro Gly Lys Glu Ala His Trp Pro Arg Tyr Pro Pro Pro Leu Met Lys 
                165                 170                 175     


Leu Tyr Ala Leu Glu Leu His Cys Ile Ile Leu Val Pro 
            180                 185                 


<210>  91
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.22; 
       tr|E2D879|E2D879_MUSMI polypeptide sequence

<400>  91

Arg Asn Leu Ile Ser Arg Glu Thr Phe Asn Phe Asn Phe Glu Asn Leu 
1               5                   10                  15      


Cys Tyr Ala Lys Gly Arg Lys Asn Thr Phe Leu Cys Tyr Glu Val Thr 
            20                  25                  30          


Arg Lys Asp Cys Asp Ser Pro Val Ser Leu Cys His Gly Val Phe Lys 
        35                  40                  45              


Asn Lys Gly Ser Ile His Ala Glu Ile Cys Phe Leu Tyr Trp Phe His 
    50                  55                  60                  


Asp Lys Val Leu Lys Val Leu Thr Pro Arg Glu Glu Phe Lys Val Thr 
65                  70                  75                  80  


Trp Tyr Met Ser Trp Ser Pro Cys Phe Glu Cys Ala Glu Gln Val Val 
                85                  90                  95      


Arg Phe Leu Ala Thr His His Asn Leu Asn Leu Thr Ile Phe Ser Ser 
            100                 105                 110         


Arg Leu Tyr Asn Val Ser Asp Pro Asp Thr Gln Gln Lys Leu Cys Arg 
        115                 120                 125             


Leu Val Gln Glu Gly Ala Gln Val Ala Val Met Asp Leu Ser Glu Phe 
    130                 135                 140                 


Lys Lys Cys Trp Glu Lys Phe Val Asp Asn Asp Gly Gln Gln Phe Arg 
145                 150                 155                 160 


Pro Trp Lys Arg Leu Arg Thr Asn Phe Arg Tyr Gln Asn Ser Lys Leu 
                165                 170                 175     


Gln Glu Ile Leu 
            180 


<210>  92
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.23; 
       tr|A0A2K5RDN6|A0A2K5RDN6_CEBCA polypeptide sequence

<400>  92

Met Trp Glu Ala Gln Ser Pro Gly Leu Ser Arg Glu Trp Gly Ser Val 
1               5                   10                  15      


Ala Ile Ser Pro Glu Asp Pro Gly Pro Leu His Ile Gly Arg Phe Leu 
            20                  25                  30          


Ser Cys Ala Phe Arg His Pro Met Asn Ala Met Tyr Pro Gly Ile Phe 
        35                  40                  45              


Asn Phe His Phe Arg Asn Leu Arg Lys Ala Tyr Gly Arg Asn Glu Thr 
    50                  55                  60                  


Trp Leu Cys Phe Thr Val Glu Gly Ile Met Asn Arg Ser Thr Val Ser 
65                  70                  75                  80  


Trp Lys Ser Gly Val Phe Arg Asn Gln Val Gly Ser Asp Pro Phe Cys 
                85                  90                  95      


His Ala Glu Met Cys Phe Leu Ser Trp Phe Arg His Asn Met Leu Ser 
            100                 105                 110         


Pro Lys Lys Asp Tyr Glu Val Thr Trp Tyr Ala Ser Trp Ser Pro Cys 
        115                 120                 125             


Pro Glu Cys Ala Gly Gln Val Ala Glu Phe Leu Ala Arg His Gly Asn 
    130                 135                 140                 


Val Arg Leu Thr Ile Phe Thr Ala His Leu Tyr Tyr Phe Trp Asn Pro 
145                 150                 155                 160 


Ser Phe Arg Gln Gly Leu Arg Arg Leu Ser Gln Glu Gly Ala Ser Val 
                165                 170                 175     


Leu Ile Met Gly Tyr Glu Asp Phe Glu Tyr Cys Trp Asp Asn Phe Val 
            180                 185                 190         


Tyr Asn Asp Gly Gln Pro Phe Lys Pro Trp Lys Arg Leu Gln Asp Asn 
        195                 200                 205             


Ser Leu Ser Leu Tyr Ile Thr Leu Gln Glu Ile Leu Gln 
    210                 215                 220     


<210>  93
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.24; 
       tr|A0A2K5RDN7|A0A2K5RDN7_CEBCA polypeptide sequence

<400>  93

Met Glu Ala Ser Pro Ala Ser Arg Pro Arg Pro Leu Met Gly Pro Arg 
1               5                   10                  15      


Thr Phe Thr Glu Asn Phe Thr Asn Asn Pro Glu Val Phe Gly Arg His 
            20                  25                  30          


Gln Thr Tyr Leu Cys Tyr Glu Val Lys Cys Gln Gly Pro Asp Gly Thr 
        35                  40                  45              


Arg Asp Leu Met Thr Glu Gln Arg Asp Phe Leu Cys Asn Gln Ala Arg 
    50                  55                  60                  


Asn Leu Leu Ser Gly Phe Asp Gly Arg His Ala Glu Arg Cys Phe Leu 
65                  70                  75                  80  


Asp Arg Val Pro Ser Trp Arg Leu Asp Pro Ala Gln Thr Tyr Arg Val 
                85                  90                  95      


Thr Cys Phe Ile Ser Trp Ser Pro Cys Phe Ser Cys Ala Arg Glu Val 
            100                 105                 110         


Ala Glu Phe Leu Gln Glu Asn Pro His Val Asn Leu Arg Ile Phe Ala 
        115                 120                 125             


Ala Arg Ile Tyr Asp Cys Arg Pro Arg Tyr Glu Glu Gly Leu Gln Met 
    130                 135                 140                 


Leu Gln Asn Ala Gly Ala Gln Val Ser Ile Met Thr Ser Glu Glu Phe 
145                 150                 155                 160 


Arg His Cys Trp Asp Thr Phe Val Asp His Gln Gly His Pro Phe Gln 
                165                 170                 175     


Pro Trp Glu Gly Leu Asp Glu His Ser Gln Ala Leu Ser Arg Arg Leu 
            180                 185                 190         


Gln Ala Ile Leu Gln Gly Asn Arg Trp Met Ile Leu Ser Leu 
        195                 200                 205     


<210>  94
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.25; 
       tr|A0A1C9CJ69|A0A1C9CJ69_CERAL polypeptide sequence

<400>  94

Asn Pro Met Lys Ala Met Asp Pro His Ile Phe Tyr Phe His Phe Lys 
1               5                   10                  15      


Asn Leu Arg Lys Ala Tyr Gly Arg Asn Glu Thr Trp Leu Cys Phe Ala 
            20                  25                  30          


Val Glu Ile Ile Lys Gln Arg Ser Thr Val Pro Trp Arg Thr Gly Val 
        35                  40                  45              


Phe Arg Asn Gln Val Asp Pro Glu Ser His Cys His Ala Glu Arg Cys 
    50                  55                  60                  


Phe Leu Ser Trp Phe Cys Glu Asp Ile Leu Ser Pro Asn Thr Asp Tyr 
65                  70                  75                  80  


Arg Val Thr Trp Tyr Thr Ser Trp Ser Pro Cys Leu Asp Cys Ala Gly 
                85                  90                  95      


Glu Val Ala Glu Phe Leu Ala Arg His Ser Asn Val Glu Leu Ala Ile 
            100                 105                 110         


Phe Ala Ala Arg Leu Tyr Tyr Phe Trp Asp Thr His Tyr Gln Gln Gly 
        115                 120                 125             


Leu Arg Ser Leu Ser Glu Lys Gly Ala Ser Val Glu Ile Met Gly Tyr 
    130                 135                 140                 


Glu Asp Phe Lys Tyr Cys Arg Glu Asn Phe Val Cys Asp Asp Gly Lys 
145                 150                 155                 160 


Pro Phe Lys Pro Trp Lys Gly Leu Lys Thr Asn Phe Arg Phe Leu Lys 
                165                 170                 175     


Arg Arg Leu Gln Glu Ile Leu Glu 
            180                 


<210>  95
<211>  197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.26; 
       tr|A0A2R2Z4D2|A0A2R2Z4D2_PTEAL polypeptide sequence

<400>  95

Met His Leu Gln Val Trp Arg Lys Val Thr Glu Ala Trp Arg Glu Gly 
1               5                   10                  15      


Tyr Thr Leu Lys Pro Trp Ser Arg Asn Pro Met Glu Arg Leu Tyr His 
            20                  25                  30          


Asp Tyr Phe Tyr Phe His Phe Tyr Asn Leu Pro Thr Pro Lys His Arg 
        35                  40                  45              


Asn Gly Cys Tyr Ile Cys Tyr Gln Val Glu Gly Thr Lys Lys His Ser 
    50                  55                  60                  


Arg Met Pro Leu Leu Arg Gly Val Phe Glu Asn Gln Glu Ser Leu Asp 
65                  70                  75                  80  


Met Met Leu Ser Pro Gly Glu Lys Tyr Arg Val Thr Trp Tyr Ile Ser 
                85                  90                  95      


Trp Ser Pro Cys Phe Ala Cys Val Asp Glu Val Ile Lys Phe Leu Arg 
            100                 105                 110         


Glu His Thr Asn Val Glu Leu Ile Ile Phe Ala Ala Arg Leu Tyr His 
        115                 120                 125             


Ser Asp Ile Leu Gln Tyr Arg Gln Gly Leu Arg Lys Leu His Asp Ala 
    130                 135                 140                 


Gly Val His Val Ala Ile Met Ser Tyr Tyr Glu Phe Lys His Cys Leu 
145                 150                 155                 160 


Asn Asp Phe Val Phe His Gln Gly Arg Ser Phe Cys Pro Trp Asn Asp 
                165                 170                 175     


Leu Asn Lys Asn Ser Lys Asn Leu Ser Asn Thr Leu Glu Asp Ile Leu 
            180                 185                 190         


Arg Asn Gln Glu Asp 
        195         


<210>  96
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.27; 
       tr|B7T161|B7T161_SHEEP polypeptide sequence

<400>  96

Met Thr Glu Gly Trp Ala Gly Ser Gly Leu Pro Gly Arg Gly Asp Cys 
1               5                   10                  15      


Val Trp Thr Pro Gln Thr Arg Asn Thr Met Asn Leu Leu Arg Glu Thr 
            20                  25                  30          


Leu Phe Lys Gln Gln Phe Gly Asn Gln Pro Arg Val Pro Pro Pro Tyr 
        35                  40                  45              


Tyr Arg Arg Lys Thr Tyr Leu Cys Tyr Gln Leu Lys Glu Leu Asp Asp 
    50                  55                  60                  


Leu Met Leu Asp Lys Gly Cys Phe Arg Asn Lys Lys Gln Arg His Ala 
65                  70                  75                  80  


Glu Ile Arg Phe Ile Asp Lys Ile Asn Ser Leu Asn Leu Asn Pro Ser 
                85                  90                  95      


Gln Ser Tyr Lys Ile Ile Cys Tyr Ile Thr Trp Ser Pro Cys Pro Asn 
            100                 105                 110         


Cys Ala Ser Glu Leu Val Asp Phe Ile Thr Arg Asn Asp His Leu Asn 
        115                 120                 125             


Leu Gln Ile Phe Ala Ser Arg Leu Tyr Phe His Trp Ile Lys Pro Phe 
    130                 135                 140                 


Cys Arg Gly Leu His Gln Leu Gln Lys Ala Gly Ile Ser Val Ala Val 
145                 150                 155                 160 


Met Thr His Thr Glu Phe Glu Asp Cys Trp Glu Gln Phe Val Asp Asn 
                165                 170                 175     


Gln Leu Arg Pro Phe Gln Pro Trp Asp Lys Leu Glu Gln Tyr Ser Ala 
            180                 185                 190         


Ser Ile Arg Arg Arg Leu Gln Arg Ile Leu Thr Ala Pro Thr 
        195                 200                 205     


<210>  97
<211>  205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.28; 
       tr|A0A2R2X2G4|A0A2R2X2G4_PTEAL polypeptide sequence

<400>  97

Met Ala Gly Leu Gly Gln Ala Cys Glu Gly Cys Cys Gly Gln Met Pro 
1               5                   10                  15      


Glu Ile Ser Tyr Pro Met Gly Arg Leu Asp Pro Lys Thr Phe Ser Phe 
            20                  25                  30          


Glu Phe Lys Asn Leu Pro Tyr Ala Tyr Gly Arg Lys Ser Ser Tyr Leu 
        35                  40                  45              


Cys Phe Gln Val Glu Arg Glu Gln His Ser Ser Pro Val Pro Ser Asp 
    50                  55                  60                  


Trp Gly Val Phe Lys Asn Gln Phe Cys Gly Thr Glu Pro Tyr His Ala 
65                  70                  75                  80  


Glu Leu Cys Phe Leu Asn Trp Phe Arg Ala Glu Lys Leu Ser Pro Tyr 
                85                  90                  95      


Glu His Tyr Asp Val Thr Trp Phe Leu Ser Trp Ser Pro Cys Ser Thr 
            100                 105                 110         


Cys Ala Glu Glu Ile Ala Ile Phe Leu Ser Asn His Lys Asn Val Arg 
        115                 120                 125             


Leu Asn Ile Phe Val Ser Arg Ile Tyr Tyr Phe Trp Lys Pro Ala Phe 
    130                 135                 140                 


Arg Gln Gly Leu Gln Glu Leu Asp His Leu Gly Val Gln Leu Asp Ala 
145                 150                 155                 160 


Met Ser Phe Asp Glu Phe Lys Tyr Cys Trp Glu Asn Phe Val Asp Asn 
                165                 170                 175     


Gln Gly Met Pro Phe Arg Cys Trp Lys Lys Val His Gln Asn Tyr Lys 
            180                 185                 190         


Ser Val Leu Arg Lys Leu Asn Glu Ile Leu Arg Arg Arg 
        195                 200                 205 


<210>  98
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.29; 
       tr|G1Q1M4|G1Q1M4_MYOLU polypeptide sequence

<400>  98

Tyr Ala Glu Leu Ser Phe Leu Asp Leu Phe Gln Ser Trp Asn Leu Asp 
1               5                   10                  15      


Arg Gly Arg Gln Tyr Arg Leu Thr Trp Tyr Met Ser Trp Ser Pro Tyr 
            20                  25                  30          


Pro Asp Cys Ala Gln Lys Leu Val Glu Phe Leu Gly Glu Asn Ser His 
        35                  40                  45              


Val Thr Leu Arg Ile Phe Ala Ala Asp Ile His Ser Leu Cys Ser Gly 
    50                  55                  60                  


Tyr Glu Asp Gly Leu Arg Lys Leu Arg Asp Ala Arg Ala Gln Leu Ala 
65                  70                  75                  80  


Ile Met Thr Arg Asp Glu Leu Gln Tyr Cys Trp Val Thr Phe Val Asp 
                85                  90                  95      


Asn Gln Gly Gln Pro Phe Arg Pro Trp Pro Asn Leu Val Glu His Ile 
            100                 105                 110         


Lys Thr Lys Lys Gln Glu Leu Lys Asp Ile Leu Gly Asn Pro Met Arg 
        115                 120                 125             


Arg Met Tyr Pro Lys Thr Phe Asn Phe Asn Phe Gln Asn Leu Asn Ser 
    130                 135                 140                 


Tyr Gly Arg Lys Ser Thr Phe Leu Cys Phe Glu Val Glu Thr Trp Glu 
145                 150                 155                 160 


Asp Gly Ser Val Leu Asp Tyr Gln Asn Gly Val Phe Gln Asn Gln Leu 
                165                 170                 175     


Asp Pro Gly His Ala Glu Leu Cys Phe Ile Glu Trp Phe His Glu Lys 
            180                 185                 190         


Val Leu Phe Pro Asp Glu Val Arg Cys Pro Asp Ala Gln Tyr His Val 
        195                 200                 205             


Thr Trp Tyr Ile Ser Trp Ser Pro Cys Phe Glu Cys Ala Glu Gln Val 
    210                 215                 220                 


Ala Gly Phe Leu Asn Glu His Glu Asn Val Asp Leu Ser Ile Ser Ala 
225                 230                 235                 240 


Ala Arg Leu Tyr Leu Cys Glu Asp Glu Asp Glu Gln Gly Leu Gln Asp 
                245                 250                 255     


Leu Val Ala Ala Gly Ala Lys Val Ala Met Met Ala Pro Glu Asp Phe 
            260                 265                 270         


Glu Tyr Cys Trp Asp Asn Phe Val Tyr Asn Arg Gly Trp Pro Phe Thr 
        275                 280                 285             


Tyr Trp Lys His Val Arg Arg Asn Tyr Gly Arg Leu Gln Glu Lys Leu 
    290                 295                 300                 


Asp Glu Ile Leu Trp 
305                 


<210>  99
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.30; 
       tr|A0A1S3AN78|A0A1S3AN78_ERIEU polypeptide sequence

<400>  99

Arg Arg Ile Glu Pro Trp Glu Phe Glu Asp Phe Phe Asp Pro Arg Gln 
1               5                   10                  15      


Phe Arg Pro Glu Thr Cys Leu Leu Tyr Glu Val Arg Trp Gly Ser Ser 
            20                  25                  30          


Arg Asn Ala Trp Arg Ser Thr Ala Arg Asn Thr Thr Arg His Ala Glu 
        35                  40                  45              


Val Asn Phe Leu Glu Arg Phe Ala Ala Glu Arg His Phe Asp Lys Pro 
    50                  55                  60                  


Val Ser Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Trp Glu 
65                  70                  75                  80  


Cys Ser Gln Ala Ile Gly Ala Phe Leu Ser Gln His Pro Gln Val Thr 
                85                  90                  95      


Leu Ala Ile His Val Thr Arg Leu Phe His His Glu Asp Glu Gln Asn 
            100                 105                 110         


Arg Gln Gly Leu Arg Asp Leu Leu Ala Arg Gly Val Thr Leu Gln Val 
        115                 120                 125             


Met Gly Asp Ser Glu Tyr Ala His Cys Trp Arg Thr Phe Val Asn Ser 
    130                 135                 140                 


Pro Pro Gly Ala Glu Gly His Tyr Pro Arg Tyr Pro Ser Asp Phe Thr 
145                 150                 155                 160 


Arg Leu Tyr Ala Leu Glu Leu His Cys Ile Ile Leu Gly Leu Pro Pro 
                165                 170                 175     


Cys Leu Glu Ile Leu Arg Arg Tyr Gln Asn Gln Phe Thr Leu Phe Arg 
            180                 185                 190         


Leu Val Pro Gln Asn Cys His Tyr Gln Met Ile Pro His Leu Asn Phe 
        195                 200                 205             


Phe Val Val Arg His Tyr Phe Phe 
    210                 215     


<210>  100
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.32; 
       tr|Q4VUI3|Q4VUI3_XENLA polypeptide sequence

<400>  100

Met Thr Met Asp Ser Met Leu Leu Lys Arg Asn Lys Phe Ile Tyr His 
1               5                   10                  15      


Tyr Lys Asn Leu Arg Trp Ala Arg Gly Arg His Glu Thr Tyr Leu Cys 
            20                  25                  30          


Tyr Ile Val Lys Arg Arg Tyr Ser Ser Val Ser Cys Ala Leu Asp Phe 
        35                  40                  45              


Gly Tyr Leu Arg Asn Arg Asn Gly Cys His Ala Glu Met Leu Phe Leu 
    50                  55                  60                  


Arg Tyr Leu Ser Ile Trp Val Gly His Asp Pro His Arg Asn Tyr Arg 
65                  70                  75                  80  


Val Thr Trp Phe Ser Ser Trp Ser Pro Cys Tyr Asp Cys Ala Lys Arg 
                85                  90                  95      


Thr Leu Glu Phe Leu Lys Gly His Pro Asn Phe Ser Leu Arg Ile Phe 
            100                 105                 110         


Ser Ala Arg Leu Tyr Phe Cys Glu Glu Arg Asn Ala Glu Pro Glu Gly 
        115                 120                 125             


Leu Arg Lys Leu Gln Lys Ala Gly Val Arg Leu Ser Val Met Ser Tyr 
    130                 135                 140                 


Lys Asp Tyr Phe Tyr Cys Trp Asn Thr Phe Val Glu Thr Arg Glu Ser 
145                 150                 155                 160 


Gly Phe Glu Ala Trp Asp Gly Leu His Glu Asn Ser Val Arg Leu Ala 
                165                 170                 175     


Arg Lys Leu Arg Arg Ile Leu Gln Pro Pro Tyr Asp Met Glu Asp Leu 
            180                 185                 190         


Arg Glu Val Phe Val Leu Leu Gly Leu 
        195                 200     


<210>  101
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.33; 
       tr|E2RL86|E2RL86_CANLF polypeptide sequence

<400>  101

Met Asn Pro Leu Gln Glu Glu Thr Phe Tyr Gln Gln Phe Ser Asn Gln 
1               5                   10                  15      


Arg Val Pro Lys Pro Thr Tyr Gln Arg Arg Thr Tyr Leu Cys Tyr Gln 
            20                  25                  30          


Leu Lys Pro His Glu Gly Ser Val Ile Ala Lys Val Cys Leu Gln Asn 
        35                  40                  45              


Gln Glu Lys Arg His Ala Glu Ile Cys Phe Ile Asp Asp Ile Lys Ser 
    50                  55                  60                  


Arg Gln Leu Asp Pro Ser Gln Lys Phe Glu Ile Thr Cys Tyr Val Thr 
65                  70                  75                  80  


Trp Ser Pro Cys Pro Thr Cys Ala Lys Lys Leu Ile Ala Phe Val Asn 
                85                  90                  95      


Asp His Pro His Ile Ser Leu Arg Leu Phe Ala Ser Arg Leu Tyr Phe 
            100                 105                 110         


His Trp Arg Gln Lys Tyr Lys Arg Glu Leu Arg His Leu Gln Lys Ser 
        115                 120                 125             


Gly Ile Pro Leu Ala Val Met Ser Tyr Leu Glu Phe Lys Asp Cys Trp 
    130                 135                 140                 


Glu Lys Phe Val Asp His Lys Gly Arg Pro Phe Gln Pro Trp Asn Lys 
145                 150                 155                 160 


Leu Lys Gln Tyr Ser Glu Ser Ile Gly Arg Arg Leu Gln Arg Ile Leu 
                165                 170                 175     


Gln Pro Leu Asn Asn Leu Glu Asn Asp Phe Arg Asn Leu Arg Leu 
            180                 185                 190     


<210>  102
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.34; 
       tr|G1LWB0|G1LWB0_AILME polypeptide sequence

<400>  102

Ser Ser Ala Ala Pro Ala Ser Ile His Leu Leu Asp Glu Asp Thr Phe 
1               5                   10                  15      


Thr Glu Asn Phe Arg Asn Asp Asp Trp Pro Ser Arg Thr Tyr Leu Cys 
            20                  25                  30          


Tyr Lys Val Glu Gly Pro Asp Gln Gly Ser Gly Val Pro Leu Gly Gln 
        35                  40                  45              


Asp Lys Gly Ile Leu His Asn Lys Pro Ala Gln Gly Pro Glu Pro Ser 
    50                  55                  60                  


Arg His Ala Glu Cys Tyr Leu Leu Glu Gln Ile Gln Ser Trp Asn Leu 
65                  70                  75                  80  


Asp Pro Lys Leu His Tyr Gly Val Thr Cys Phe Leu Ser Trp Ser Pro 
                85                  90                  95      


Cys Ala Lys Cys Ala Gln Lys Met Ala Arg Phe Leu Gln Glu Asn Ser 
            100                 105                 110         


His Val Ser Leu Lys Leu Phe Ala Ser Arg Leu Tyr Thr Arg Glu Arg 
        115                 120                 125             


Trp Asp Glu Asp Tyr Lys Glu Gly Leu Arg Thr Leu Lys Arg Ala Gly 
    130                 135                 140                 


Ala Ser Ile Ala Ile Met Thr Tyr Arg Glu Phe Glu His Cys Trp Lys 
145                 150                 155                 160 


Thr Phe Val Leu His Asp Gln Glu Gly Ser Cys Phe Gln Pro Trp Pro 
                165                 170                 175     


Phe Leu His Lys Glu Ser Gln Lys Phe Ser Glu Lys Leu Gln Ala Ile 
            180                 185                 190         


Leu Gln Val Gly Val Leu Leu Leu Ser Leu Pro Pro Pro Leu Pro Ser 
        195                 200                 205             


Ser Pro Leu Ser Ser Pro Trp Pro Phe Pro Ala Pro Leu Arg Ala Ser 
    210                 215                 220                 


Thr Gly 
225     


<210>  103
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.35; 
       tr|A0A1U7S7K7|A0A1U7S7K7_ALLSI polypeptide sequence

<400>  103

Met Gly Glu His Trp Gln Tyr Ala Gly Ser Gly Glu Tyr Ile Pro Gln 
1               5                   10                  15      


Asp Gln Phe Glu Glu Asn Phe Asp Pro Ser Val Leu Leu Ala Glu Thr 
            20                  25                  30          


His Leu Leu Ser Glu Leu Thr Trp Gly Gly Arg Pro Tyr Lys His Trp 
        35                  40                  45              


Tyr Glu Asn Thr Glu His Cys His Ala Glu Ile His Phe Leu Glu Asn 
    50                  55                  60                  


Phe Ser Ser Lys Asn Arg Ser Cys Thr Ile Thr Trp Tyr Leu Ser Trp 
65                  70                  75                  80  


Ser Pro Cys Ala Glu Cys Ser Ala Arg Ile Ala Asp Phe Met Gln Glu 
                85                  90                  95      


Asn Thr Asn Val Lys Leu Asn Ile His Val Ala Arg Leu Tyr Leu His 
            100                 105                 110         


Asp Asp Glu His Thr Arg Gln Gly Leu Arg Tyr Leu Met Lys Met Lys 
        115                 120                 125             


Arg Val Thr Ile Gln Val Met Thr Ile Pro Asp Tyr Thr Tyr Cys Trp 
    130                 135                 140                 


Asn Thr Phe Leu Glu Asp Asp Gly Glu Asp Glu Ser Asp Asp Tyr Gly 
145                 150                 155                 160 


Gly Tyr Ala Gly Val His Glu Asp Glu Asp Glu Ser Asp Asp Asp Asp 
                165                 170                 175     


Tyr Leu Pro Thr His Phe Ala Pro Trp Ile Met Leu Tyr Ser Leu Glu 
            180                 185                 190         


Leu Ser Cys Ile Leu Gln Gly Phe Ala Pro Cys Leu Lys Ile Ile Gln 
        195                 200                 205             


Gly Asn His Met Ser Pro Thr Phe Gln Leu His Val Gln Asp Gln Glu 
    210                 215                 220                 


Gln Lys Arg Leu Leu Glu Pro Ala Asn Pro Trp Gly Ala Asp 
225                 230                 235             


<210>  104
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.36; 
       tr|A0A2R2X2J8|A0A2R2X2J8_PTEVA polypeptide sequence

<400>  104

Met Pro Arg Ile Gly Asn Met Asn Leu Leu Ser Glu Lys Thr Phe Asn 
1               5                   10                  15      


Tyr His Phe Gly Asn Gln Leu Arg Val Lys Lys Pro Gln Gly Arg Arg 
            20                  25                  30          


Arg Thr Tyr Leu Cys Tyr Lys Leu Lys Leu Pro Asn Glu Thr Leu Val 
        35                  40                  45              


Lys Gly Tyr Phe Ile Asn Lys Lys Lys Asn His Ala Glu Ile Arg Phe 
    50                  55                  60                  


Ile Asn Lys Ile Arg Ser Leu Asn Leu Asp Gln Thr Gln Ser Tyr Lys 
65                  70                  75                  80  


Ile Thr Cys Tyr Ile Thr Trp Ser Pro Cys Ser Tyr Cys Ala Gly Lys 
                85                  90                  95      


Leu Val Ala Leu Val Lys Ser Cys Pro His Leu Ser Leu Gln Ile Phe 
            100                 105                 110         


Thr Ser Arg Leu Tyr Tyr His Trp Leu Trp Lys Asn Gln Ala Gly Leu 
        115                 120                 125             


Arg Tyr Leu Trp Lys Ile Asn Ile Ser Val Leu Val Met Lys Glu Pro 
    130                 135                 140                 


Glu Phe Ala Asp Cys Trp Asp Asn Phe Val Asn His Gln Ser Arg Arg 
145                 150                 155                 160 


Phe Lys Pro Trp Glu Lys Leu Thr Gln Tyr Ser Asn Ser Thr Glu Arg 
                165                 170                 175     


Arg Leu Leu Arg Ile Leu Arg Ile Asn Arg Thr Asp Leu Phe Leu Ala 
            180                 185                 190         


Gln Ser Ser Glu Gln Asp Pro Gly Leu Asn Asp Leu Val Asp Ala Ile 
        195                 200                 205             


Lys Arg Leu Phe Leu Asp Ala His Arg Pro Arg Asp 
    210                 215                 220 


<210>  105
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.37; 
       tr|A0A151P6M4|A0A151P6M4_ALLMI polypeptide sequence

<400>  105

Met Ala Val Glu Glu Glu Lys Gly Leu Leu Gly Thr Ser Gln Gly Trp 
1               5                   10                  15      


Lys Ile Glu Leu Lys Asp Phe Gln Glu Asn Tyr Met Pro Ser Thr Trp 
            20                  25                  30          


Pro Lys Val Thr His Leu Leu Tyr Glu Ile Arg Trp Gly Lys Gly Ser 
        35                  40                  45              


Lys Val Trp Arg Asn Trp Cys Ser Asn Thr Leu Thr Gln His Ala Glu 
    50                  55                  60                  


Val Asn Cys Leu Glu Asn Ala Phe Gly Lys Leu Gln Phe Asn Pro Pro 
65                  70                  75                  80  


Val Pro Cys His Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Cys Gln 
                85                  90                  95      


Cys Cys Arg Arg Ile Leu Gln Phe Leu Arg Ala His Ser His Ile Thr 
            100                 105                 110         


Leu Val Ile Lys Ala Ala Gln Leu Phe Lys His Met Asp Glu Arg Asn 
        115                 120                 125             


Arg Gln Gly Leu Arg Asp Leu Val Gln Ser Gly Val His Val Gln Val 
    130                 135                 140                 


Met Asp Leu Pro Asp Tyr Arg Tyr Cys Trp Arg Thr Phe Val Ser His 
145                 150                 155                 160 


Pro His Glu Gly Glu Gly Asp Phe Trp Pro Trp Phe Phe Pro Leu Trp 
                165                 170                 175     


Ile Thr Phe Tyr Thr Leu Glu Leu Gln His Ile Leu Leu Gln Gln His 
            180                 185                 190         


Ala Leu Ser Tyr Asn Leu 
        195             


<210>  106
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.38; 
       tr|A0A2K6MNR2|A0A2K6MNR2_RHIBE polypeptide sequence

<400>  106

Ile Trp Leu Cys Phe Thr Met Glu Ile Ile Lys Gln Cys Ser Thr Val 
1               5                   10                  15      


Ser Trp Lys Arg Gly Val Phe Arg Asn Gln Val Asp Pro Glu Thr His 
            20                  25                  30          


Cys His Ala Glu Arg Cys Phe Leu Ser Trp Phe Trp Glu Asp Thr Leu 
        35                  40                  45              


Ser Pro Asn Thr Asn Tyr Gln Val Thr Trp Tyr Thr Ser Trp Ser Pro 
    50                  55                  60                  


Cys Leu Asp Cys Ala Gly Glu Val Ala Glu Phe Leu Ala Arg His Ser 
65                  70                  75                  80  


Asn Val Lys Leu Ala Ile Phe Ala Ala Arg Leu Tyr Tyr Phe Trp Asp 
                85                  90                  95      


Thr Asp Tyr Gln Gln Gly Leu Arg Ser Leu Ser Glu Glu Gly Thr Ser 
            100                 105                 110         


Val Glu Ile Met Gly Tyr Glu Asp Phe Lys Tyr Cys Trp Glu Asn Phe 
        115                 120                 125             


Val Tyr Asn Gly Asp Glu Pro Phe Lys Pro Trp Lys Gly Leu Lys Tyr 
    130                 135                 140                 


Asn Phe Leu Phe Leu Asp Ser Lys Leu Gln Glu Ile Leu Glu 
145                 150                 155             


<210>  107
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.40; 
       tr|F1CGT0|F1CGT0_ANOCA polypeptide sequence

<400>  107

Lys Ala Ala Ile Leu Leu Ser Asn Leu Phe Phe Arg Trp Gln Met Glu 
1               5                   10                  15      


Pro Glu Ala Phe Gln Arg Asn Phe Asp Pro Arg Glu Phe Pro Glu Cys 
            20                  25                  30          


Thr Leu Leu Leu Tyr Glu Ile His Trp Asp Asn Asn Thr Ser Arg Asn 
        35                  40                  45              


Trp Cys Thr Asn Lys Pro Gly Leu His Ala Glu Glu Asn Phe Leu Gln 
    50                  55                  60                  


Ile Phe Asn Glu Lys Ile Asp Ile Lys Gln Asp Thr Pro Cys Ser Ile 
65                  70                  75                  80  


Thr Trp Phe Leu Ser Trp Ser Pro Cys Tyr Pro Cys Ser Gln Ala Ile 
                85                  90                  95      


Ile Lys Phe Leu Glu Ala His Pro Asn Val Ser Leu Glu Ile Lys Ala 
            100                 105                 110         


Ala Arg Leu Tyr Met His Gln Ile Asp Cys Asn Lys Glu Gly Leu Arg 
        115                 120                 125             


Asn Leu Gly Arg Asn Arg Val Ser Ile Met Asn Leu Pro Asp Tyr Arg 
    130                 135                 140                 


His Cys Trp Thr Thr Phe Val Val Pro Arg Gly Ala Asn Glu Asp Tyr 
145                 150                 155                 160 


Trp Pro Gln Asp Phe Leu Pro Ala Ile Thr Asn Tyr Ser Arg Glu Leu 
                165                 170                 175     


Asp Ser Ile Leu Gln Asp 
            180         


<210>  108
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.41; 
       tr|C7AGG3|C7AGG3_HORSE polypeptide sequence

<400>  108

Met Asp Pro Gln Ala Pro Thr Gln Arg Gly Gly Leu Gly Gln Ala Tyr 
1               5                   10                  15      


Gln Gly Gly Asp Tyr Val Gln Ala Pro Gly Asn Gly Asn Thr Gln His 
            20                  25                  30          


Leu Leu Ser Glu Asp Val Phe Lys Lys Gln Phe Gly Asn Gln Arg Arg 
        35                  40                  45              


Val Thr Lys Pro Tyr Tyr Arg Arg Lys Thr Tyr Val Cys Tyr Gln Leu 
    50                  55                  60                  


Lys Leu Leu Arg Gly Pro Thr Ile Ala Lys Gly Tyr Phe Arg Asn Lys 
65                  70                  75                  80  


Lys Lys Arg His Ala Glu Ile Arg Phe Ile Asp Lys Ile Asn Ser Leu 
                85                  90                  95      


Gly Leu Asp Gln Asp Gln Ser Tyr Glu Ile Thr Cys Tyr Val Thr Trp 
            100                 105                 110         


Ser Pro Cys Ala Thr Cys Ala Cys Lys Leu Ile Lys Phe Thr Arg Lys 
        115                 120                 125             


Phe Pro Asn Leu Ser Leu Arg Ile Phe Val Ser Arg Leu Tyr Tyr His 
    130                 135                 140                 


Trp Phe Arg Gln Asn Gln Gln Gly Leu Arg Gln Leu Trp Ala Ser Ser 
145                 150                 155                 160 


Ile Pro Val Val Val Met Gly Tyr Gln Glu Phe Ala Asp Cys Trp Glu 
                165                 170                 175     


Asn Phe Ala Asp Asn Arg Gly Asn Pro Phe Gln Ser Trp Glu Lys Leu 
            180                 185                 190         


Thr Glu Tyr Ser Lys Gly Ile Lys Arg Arg Leu Gln Lys Ile Leu Glu 
        195                 200                 205             


Pro Leu Asn Leu Asn Gly Leu Glu Asp Ala Met Gly Asn Leu Lys Leu 
    210                 215                 220                 


Gly Ser Val Asp Leu Gly 
225                 230 


<210>  109
<211>  197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.42; 
       tr|A0A250YMK7|A0A250YMK7_CASCN polypeptide sequence

<400>  109

Met Ser Leu Leu Lys Glu Asp Ile Phe Leu Tyr Gln Phe Asn Asn Gln 
1               5                   10                  15      


Gln Gln Val Gln Lys Pro Tyr Phe Arg Arg Arg Thr Tyr Leu Cys Tyr 
            20                  25                  30          


Gln Leu Glu Gln Pro Asn Gly Ser Arg Pro Gln Trp Pro Ala Lys Gly 
        35                  40                  45              


Cys Leu Gln Asn Lys Lys Gly His His Ala Glu Ile Arg Phe Ile Lys 
    50                  55                  60                  


Arg Ile His Ser Met Gly Leu Glu Gln Asp Gln Asp Tyr Gln Ile Thr 
65                  70                  75                  80  


Cys Tyr Ile Thr Trp Ser Pro Cys Leu Ala Cys Ala Cys Ala Leu Ala 
                85                  90                  95      


Glu Leu Lys Asn His Phe Pro Arg Leu Thr Leu Arg Ile Phe Ala Ser 
            100                 105                 110         


Arg Leu Tyr Phe His Trp Ile Arg Lys Phe Gln Met Gly Leu Gln His 
        115                 120                 125             


Leu Tyr Lys Ser Gly Val Leu Val Ala Val Met Ser Leu Pro Glu Phe 
    130                 135                 140                 


Thr Asp Cys Trp Glu Lys Phe Val Asn His Arg Gln Val Phe Phe Thr 
145                 150                 155                 160 


Pro Trp Asp Lys Leu Glu Glu His Ser Arg Ser Ile Gln Arg Arg Leu 
                165                 170                 175     


Arg Arg Ile Leu Gln Ser Trp Asp Val Asp Asp Leu Thr Asp Asp Phe 
            180                 185                 190         


Arg Asn Leu Arg Leu 
        195         


<210>  110
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM3.43; 
       tr|B7T160|B7T160_SHEEP polypeptide sequence

<400>  110

Met Pro Trp Ile Ser Asp His Val Ala Arg Leu Asp Pro Glu Thr Phe 
1               5                   10                  15      


Tyr Phe Gln Phe His Asn Leu Leu Tyr Ala Tyr Gly Arg Asn Cys Ser 
            20                  25                  30          


Tyr Ile Cys Tyr Arg Val Lys Thr Trp Lys His Arg Ser Pro Val Ser 
        35                  40                  45              


Phe Asp Trp Gly Val Phe His Asn Gln Val Tyr Ala Gly Thr His Cys 
    50                  55                  60                  


His Ser Glu Arg Arg Phe Leu Ser Trp Phe Cys Ala Lys Lys Leu Arg 
65                  70                  75                  80  


Pro Asp Glu Cys Tyr His Ile Thr Trp Phe Met Ser Trp Ser Pro Cys 
                85                  90                  95      


Met Lys Cys Ala Glu Leu Val Ala Gly Phe Leu Gly Met Tyr Gln Asn 
            100                 105                 110         


Val Thr Leu Ser Ile Phe Thr Ala Arg Leu Tyr Tyr Phe Gln Lys Pro 
        115                 120                 125             


Gln Tyr Arg Lys Gly Leu Leu Arg Leu Ser Asp Gln Gly Ala Cys Val 
    130                 135                 140                 


Asp Ile Met Ser Tyr Gln Glu Phe Lys Tyr Cys Trp Lys Lys Phe Val 
145                 150                 155                 160 


Tyr Ser Gln Arg Arg Pro Phe Arg Pro Trp Lys Lys Leu Lys Arg Asn 
                165                 170                 175     


Tyr Gln Leu Leu Ala Ala Glu Leu Glu Asp Ile Leu Gly 
            180                 185                 


<210>  111
<211>  541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.1; 
       tr|A0A182D0J1|A0A182D0J1_BLAVI polypeptide sequence

<400>  111

Met Thr Asn Pro Glu Ser Pro Pro Gln Ala Pro Cys Asp Phe Asn Glu 
1               5                   10                  15      


Asp Ala Leu Leu Asn Arg Glu Pro Leu Arg Gly Ser Pro Ile Lys Phe 
            20                  25                  30          


Val Ser Pro Val Asp Tyr Pro Asp Leu Val Phe Ala Leu Ala Gly Pro 
        35                  40                  45              


Val Gly Val Asp Ile Asp Tyr Ile Gln Gln Ser Ile Ser Asp Cys Leu 
    50                  55                  60                  


Lys Ser Phe Asp Tyr Ser Thr Glu Phe Ile Arg Ile Thr Glu Ile Met 
65                  70                  75                  80  


Gln Asp Ile Lys Cys Ser Lys Thr Ile Asp Cys Thr Asp Met Leu Lys 
                85                  90                  95      


Glu Tyr Gln Ser Lys Ile Glu Tyr Ala Asn Glu Leu Arg Arg Ala Tyr 
            100                 105                 110         


Arg Ala Lys Asp Leu Leu Ala Ala Leu Thr Ile Ser Ala Ile Ser Lys 
        115                 120                 125             


Leu Arg Glu Gln Ile Lys Glu Arg Asp Glu Ala Thr Asn Lys Ser Asn 
    130                 135                 140                 


Ile Gln Pro Ser Arg Arg Lys Leu Ala Trp Val Val Arg Gln Leu Lys 
145                 150                 155                 160 


Thr Pro Glu Glu Val Arg Leu Leu Arg Ala Val Tyr Gly Lys Gln Phe 
                165                 170                 175     


Val Leu Val Ser Ile Tyr Ser Ser Pro Gln Arg Arg Glu Asp Phe Leu 
            180                 185                 190         


Ile Ser Lys Ile Lys Ile Lys Ser Arg Gly Thr Ile Asp Asn Asn Thr 
        195                 200                 205             


Ser Ser Glu Gly Ala Gln Arg Leu Ile Glu Arg Asp Ser Lys Glu Asp 
    210                 215                 220                 


Asn Glu Tyr Gly Gln Asn Leu Ser Gly Thr Phe Cys Leu Gly Asp Ile 
225                 230                 235                 240 


Phe Val Asp Ser Asn Asn Lys Glu Ser Ala Ile Val Ser Ile Asp Arg 
                245                 250                 255     


Phe Leu Asn Ala Phe Phe Gly Ser Asn Glu Ile Ser Pro Thr Arg Asp 
            260                 265                 270         


Glu Tyr Gly Met Tyr Leu Ala Lys Thr Ala Ser Leu Arg Ser Cys Asp 
        275                 280                 285             


Leu Ser Arg Gln Val Gly Ala Ala Ile Phe Ser Lys Thr Gly Glu Ile 
    290                 295                 300                 


Ile Ser Leu Gly Ser Asn Glu Val Pro Lys Ala Gly Gly Gly Thr Tyr 
305                 310                 315                 320 


Trp Thr Gly Asp Asn Ala Asp Ser Arg Asp Ile Arg Leu Gly His Asp 
                325                 330                 335     


Pro Asn Glu Ile Asn Lys Val Glu Ile Phe Ala Glu Ile Ile Ser Arg 
            340                 345                 350         


Leu Leu Glu Asp Lys Leu Leu Ser Asn Asp Leu Leu Asn Lys Asp Ala 
        355                 360                 365             


Ala Ser Ile Val Thr Ile Leu Leu Ser Lys Asn Glu Gly Lys Arg Tyr 
    370                 375                 380                 


Lys Asp Leu Arg Val Met Asp Ile Ile Glu Phe Gly Arg Ile Ile His 
385                 390                 395                 400 


Ala Glu Met Ser Ala Ile Cys Asp Ala Ala Arg Asn Gly Arg Ala Ile 
                405                 410                 415     


Ile Gly Ala Thr Leu Phe Cys Thr Thr Phe Pro Cys His Leu Cys Ala 
            420                 425                 430         


Lys His Ile Val Ala Ser Gly Ile Gly Arg Ile Val Tyr Leu Glu Pro 
        435                 440                 445             


Tyr Pro Lys Ser Tyr Ala Lys Lys Leu His Ser Asp Ser Ile Gln Val 
    450                 455                 460                 


Glu Asp His Ser Asp Ser Glu Lys Val Ser Phe Glu Pro Phe Ile Gly 
465                 470                 475                 480 


Ile Ser Pro Ser Arg Tyr Arg Glu Leu Phe Glu Gly Gly Arg Arg Lys 
                485                 490                 495     


Asp Pro Phe Gly Glu Ala Leu Lys Trp Lys Asn Asp Pro Arg Lys Pro 
            500                 505                 510         


Val Ile Asp Val Val Val Pro Pro His Phe Glu Ala Glu Lys Leu Val 
        515                 520                 525             


Ile Ala Gln Leu Gly Lys Leu Ile Val Ser Gly Thr Gly 
    530                 535                 540     


<210>  112
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.2; 
       tr|A0A2D6EXD2|A0A2D6EXD2_9ARCH polypeptide sequence

<400>  112

Met Ile Ile Gly Leu Val Gly Thr Ile Gly Ala Gly Lys Gln Thr Ile 
1               5                   10                  15      


Ile Asp Tyr Leu Gln Glu Lys Tyr Gly Tyr Asn Ala Leu Ser Cys Ser 
            20                  25                  30          


Asp Val Leu Arg Glu Ile Leu Lys Lys Gln Gly Lys Pro Val Thr Arg 
        35                  40                  45              


Asp Asn Leu Arg Glu Ile Gly Asn Lys Thr Arg Glu Glu Gly Gly Asn 
    50                  55                  60                  


Gly Ala Ile Ala Lys Ile Leu Leu Glu Lys Leu Arg Asn Asn Trp Lys 
65                  70                  75                  80  


Ala Asn Tyr Ile Val Asp Ser Leu Arg His Pro Asp Glu Val Ser Val 
                85                  90                  95      


Leu Arg Thr Ser Pro Leu Phe His Leu Val Ala Val Asp Ala Asp Leu 
            100                 105                 110         


Arg Ile Arg Phe Glu Arg Val Lys Ala Arg Lys Arg Glu Glu Glu Pro 
        115                 120                 125             


Thr Thr Leu Pro Ala Phe Val Glu Arg Asp Gln Lys Glu Met Phe Gly 
    130                 135                 140                 


Thr Gly Asn Glu Gln Arg Ile Arg Glu Thr Met Glu Leu Ala Asp Glu 
145                 150                 155                 160 


Leu Val Leu Asn Asn Gly Thr Val Glu Glu Leu Lys Gln Arg Ile Asp 
                165                 170                 175     


Asp Leu Asn Leu Val Ser Asp Glu Arg Leu Arg Pro Ser Trp Asp Asp 
            180                 185                 190         


Tyr Phe Met Arg Leu Ala Arg Leu Ala Ala Gln Arg Ser Asn Cys Met 
        195                 200                 205             


Ser Arg Lys Val Gly Ala Ile Ile Thr Lys Asp Arg Arg Val Ile Ala 
    210                 215                 220                 


Thr Gly Tyr Asn Gly Thr Pro Arg Gly Val Lys Asn Cys Asn Glu Gly 
225                 230                 235                 240 


Gly Cys Glu Arg Cys Asn Ser Ala Val Ala Lys Gly Thr Ala Ile Ser 
                245                 250                 255     


Glu Cys Leu Cys Leu His Gly Glu Glu Asn Ala Ile Ile Glu Ala Gly 
            260                 265                 270         


Arg Val Arg Ser Glu Gly Ala Thr Ile Tyr Thr Ser Phe Leu Pro Cys 
        275                 280                 285             


Leu Trp Cys Thr Lys Met Ile Ile Gln Ala Gly Leu Lys Glu Val Val 
    290                 295                 300                 


Phe Ser Glu Val Tyr Asp Leu His Glu Ala Ser Ile Lys Leu Phe Glu 
305                 310                 315                 320 


Thr Ser Gly Val Leu Ile Arg Arg Leu Lys 
                325                 330 


<210>  113
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.3; 
       tr|F7YVM7|F7YVM7_9THEM polypeptide sequence

<400>  113

Met Asn Glu Phe Lys Tyr Met Ser Leu Ala Leu Lys Leu Ala Lys Lys 
1               5                   10                  15      


Gly Lys Tyr Thr Thr Ser Pro Asn Pro Met Val Gly Ala Val Ile Val 
            20                  25                  30          


Lys Asp Gly Lys Ile Leu Ala Thr Gly Tyr His Lys Lys Ala Gly Gln 
        35                  40                  45              


Pro His Ala Glu Ile Asn Ala Leu Ser Lys Leu Asn Phe Gln Ala Gln 
    50                  55                  60                  


Asn Cys Glu Met Tyr Val Thr Leu Glu Pro Cys Ser His Tyr Gly Arg 
65                  70                  75                  80  


Thr Pro Pro Cys Ala Asp Ala Ile Ile Arg Ser Gly Ile Arg Lys Val 
                85                  90                  95      


Val Ile Ala Thr Leu Asp Pro Asn Pro Leu Val Asn Gly Lys Gly Val 
            100                 105                 110         


Glu Lys Leu Lys Asn Ala Gly Ile Glu Val Val Cys Gly Val Leu Glu 
        115                 120                 125             


Glu Lys Ala Lys Lys Leu Asn Glu Lys Phe Phe Lys Tyr Ile Thr Thr 
    130                 135                 140                 


Lys Ile Pro Phe Val Ala Leu Lys Ile Ala Gln Thr Leu Asp Gly Lys 
145                 150                 155                 160 


Ile Ala Leu Lys Asn Gly Glu Ser Lys Trp Ile Thr Ser Glu Lys Ser 
                165                 170                 175     


Arg Glu Tyr Val His Lys Leu Arg Met Glu Tyr Asp Ala Val Leu Thr 
            180                 185                 190         


Gly Ile Gly Thr Ile Leu Lys Asp Asp Pro Gln Leu Asn Val Arg Leu 
        195                 200                 205             


Lys Lys Val Tyr Lys Gln Pro Leu Arg Ile Ile Leu Asp Ser Lys Leu 
    210                 215                 220                 


Lys Ile Pro Leu Ser Ala Lys Val Leu Glu Asp Pro Ser Lys Val Ile 
225                 230                 235                 240 


Ile Leu Thr Thr Ala Leu Ala Asp Lys Glu Lys Leu Glu Glu Leu Arg 
                245                 250                 255     


Ser Lys Gly Val Glu Val Ile Ile Thr Asn Glu Lys Asn Gly Ile Val 
            260                 265                 270         


Asp Leu Glu Ser Ala Leu Lys Ile Leu Gly Glu Lys Lys Ile Thr Ser 
        275                 280                 285             


Val Met Val Glu Ala Gly Pro Thr Leu Leu Thr Ser Phe Leu Lys Glu 
    290                 295                 300                 


Ser Leu Phe Asp Lys Ile Tyr Leu Phe Ile Ala Pro Lys Ile Phe Gly 
305                 310                 315                 320 


Ala Asp Ser Lys Ser Val Phe Ser Glu Leu Gly Leu Glu Asp Ile Ser 
                325                 330                 335     


Lys Ser Gln Lys Phe Ser Leu Glu Ser Val Lys Lys Ile Gly Glu Asp 
            340                 345                 350         


Leu Leu Leu Glu Leu Tyr Pro Lys Gln Leu Lys Lys Leu Glu Glu 
        355                 360                 365         


<210>  114
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.4; 
       tr|A0A3M6UNF1|A0A3M6UNF1_9CNID polypeptide sequence

<400>  114

Met Glu Glu Lys Ser Glu Leu Glu Asn Glu Leu Met Arg Ser Thr Ser 
1               5                   10                  15      


Pro Lys Pro Ser Val Pro Asn Gly Ser Lys Gly Asn Glu Cys Glu Gln 
            20                  25                  30          


Arg Glu Thr Arg Ile Thr Lys Glu Asn Leu Tyr Met Val Leu Ala Leu 
        35                  40                  45              


Trp Met Glu Glu Phe Pro Val Val Glu Gln Thr Ser Ser Ala Lys Arg 
    50                  55                  60                  


Leu Asn Lys Val Gly Val Val Phe Val Leu Pro Thr Asp Arg Val Leu 
65                  70                  75                  80  


Ala Ala Asp Cys Ser Arg Asp Gly Val His Gly Val Ala Arg Val Met 
                85                  90                  95      


Val Asn His Cys Gly Lys Leu Glu Gly Cys Lys Val Phe Val Ser Arg 
            100                 105                 110         


Lys Pro Cys Ser Leu Cys Ala Lys Leu Leu Val Gln Ser Lys Val Ser 
        115                 120                 125             


Arg Val Phe Tyr Leu Pro Ile Glu Pro Glu Ser Glu Asn Lys Gly Glu 
    130                 135                 140                 


Ile Ala Arg Ala Asp Asn Leu Phe Lys Asn Ser Ser Val Gly Gln Ser 
145                 150                 155                 160 


Val Phe Val Pro Cys Val Glu Gln Lys Val Leu Asp Lys Leu Glu Asp 
                165                 170                 175     


Lys Leu Pro Lys Glu Ile Ile Thr Pro Asp Asp Ile Ser Glu Cys Arg 
            180                 185                 190         


Asp Asn Leu Leu Lys Lys Cys Gly Trp Ser Ala Glu Trp Phe Ala Arg 
        195                 200                 205             


Ala Gln Ala Ser Leu Pro Trp Pro Cys Phe Glu Gly Lys Met Lys Ser 
    210                 215                 220                 


Gln Val Asp Asn Asp Phe Lys Ser Leu Ile Lys Trp Ile Ala Val Val 
225                 230                 235                 240 


Lys Ala Pro Met Asp Lys Gly Val Ala Phe Pro Lys Val Lys Leu Thr 
                245                 250                 255     


Ser Asp Ser Arg Val Val Pro Asp Cys Asp Ala Asp Asn Phe Pro Asp 
            260                 265                 270         


Ser Lys Thr Ala Tyr His Met Met Ile Phe Ala Lys Met Leu Ala Arg 
        275                 280                 285             


Gln Thr Asp Asp Pro Lys Thr Gly Val Gly Ala Val Ile Val Arg Gly 
    290                 295                 300                 


Lys Val Pro Asp Ile Val Ser Leu Gly Trp Asn Gly Phe Pro Ser Lys 
305                 310                 315                 320 


Ala Leu Tyr Gly Glu Phe Pro Arg Ala Ser Asp Asp Asp Arg Ala Leu 
                325                 330                 335     


Gln Lys Lys Phe Pro Tyr Val Ile His Ala Glu Gln Asn Ala Leu Met 
            340                 345                 350         


Val Arg Asn Val Lys Asp Leu Thr Asp Gly Ile Leu Phe Val Thr Lys 
        355                 360                 365             


Pro Pro Cys Asp Glu Cys Ala Pro Met Ile Lys Leu Ser Gly Val Lys 
    370                 375                 380                 


Thr Ile Val Ile Gly Glu Lys Ile Glu Lys Ser Arg Gly Gly Glu Leu 
385                 390                 395                 400 


Ser Tyr Asn Leu Ile Lys Glu Tyr Ile Lys Glu Gly Ile Met Thr Cys 
                405                 410                 415     


Tyr Gln Met Glu Ala Thr Lys Thr Lys Ala Lys Arg Leu Ala Ser Asp 
            420                 425                 430         


Pro Glu Thr Arg Lys Arg Leu Lys Ser Ser Cys Ser Asn Ser Asn Asp 
        435                 440                 445             


Val 
    


<210>  115
<211>  414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.5; 
       tr|A0A2G3K826|A0A2G3K826_9BURK polypeptide sequence

<400>  115

Met Thr Lys Ile Ile Asp Asp Val Asn Thr Ala Ala Ala Ala Val Leu 
1               5                   10                  15      


Asp Gln Ala Thr Ala Ala Ala Asn Gln Thr Thr Phe Ala Val Gly Gly 
            20                  25                  30          


Val Met Val Asn Asn Gln Thr Gly Glu Val Ile Ser Ala Ile His Asn 
        35                  40                  45              


Asn Val Ile Ile Pro Leu Ser Asn Asn Val Ser Phe Thr Phe Asp Pro 
    50                  55                  60                  


Thr Ala His Gly Glu Arg Gln Leu Val Tyr Trp Tyr Tyr Ala Asn Lys 
65                  70                  75                  80  


Glu Ala Leu Lys Leu Pro Glu Pro Asn Gln Ile Thr Val Ile Thr Ser 
                85                  90                  95      


Leu Asp Pro Cys Ala Met Cys Thr Gly Ala Leu Leu Thr Ala Gly Phe 
            100                 105                 110         


Asn Val Gly Val Val Ala Ile Asp Thr Tyr Ala Gly Ile Asn Cys Ala 
        115                 120                 125             


Gln Asn Phe Gln Phe Ala Thr Leu Pro Ala Asn Leu Arg Thr Lys Ala 
    130                 135                 140                 


Gln Lys Asn Phe Gly Tyr Tyr Ala Ser Gly Ala Ala Asn Phe Lys Pro 
145                 150                 155                 160 


Leu Thr Arg Ser Tyr Val Gly Gly Pro Ser Val Ala Phe Lys Asn Gly 
                165                 170                 175     


Val Val Thr Pro Ala Asn Leu Arg Asp Cys Gly Thr Val Phe Thr Gln 
            180                 185                 190         


Ser Val Asp Thr Val Arg Asn Thr Ser Asn Ser Thr Gly Leu Ala Pro 
        195                 200                 205             


Ser Gln Met Ser Asn Pro Ala Glu Leu Pro Ser Asn Ser Ala Ile Leu 
    210                 215                 220                 


Gln Ala Tyr Arg Ala Ile Tyr Lys Lys Ala Phe Thr Ile Lys Ile Asp 
225                 230                 235                 240 


Asn Pro Arg Leu Pro Asp Ala Gln Ile Leu Thr Glu Leu Lys Ala Val 
                245                 250                 255     


Leu Ala Asp Ala Pro Asn Ala Arg Asn Ala Val Ala Phe Ile Asp Pro 
            260                 265                 270         


Phe Gly Asn Leu Val Leu Cys Met Ala Asp Ala Phe Asn Thr Ser Pro 
        275                 280                 285             


Val His Ala Ala Phe Met Asn Val Thr Gln Glu Tyr Ala Lys Thr Arg 
    290                 295                 300                 


Trp Asp Leu Met Asn Lys Tyr Ala Gln Ala Ser Thr Thr Asp Asn Pro 
305                 310                 315                 320 


Ala Leu Tyr Leu Thr His Pro Lys Tyr Gly Thr Phe Val Tyr Leu Tyr 
                325                 330                 335     


Ala Pro Asp Pro Asp Asp Ser Ile Thr Ile Met Ser Leu Gly Ala Tyr 
            340                 345                 350         


Gly Ser Thr Met Glu Gly Pro Ile Pro Asn Met Phe Pro Ser Asn Leu 
        355                 360                 365             


Gln Phe Tyr Tyr Pro Pro Arg Asn Gly Ala Gln Phe Ser Glu Leu Val 
    370                 375                 380                 


Pro Val Val Asn Glu Leu Pro Pro Phe Tyr Thr Gln Asn Val Asn Ile 
385                 390                 395                 400 


Ser Leu Met Gln Val Pro Gly Val Thr Gln Ala Pro Thr Lys 
                405                 410                 


<210>  116
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.6; 
       tr|K1ZCJ4|K1ZCJ4_9BACT polypeptide sequence

<400>  116

Met Ser Ser Arg Ala Lys Lys Asn Arg Ser Thr Asn Leu Lys Lys Ser 
1               5                   10                  15      


Ile Gly Gln Lys Ser Ile Glu Asn Lys Pro Thr Asp Gln Lys Lys Asp 
            20                  25                  30          


Gln Val Leu Val Ala Tyr Val Pro Val Ile His Glu Gly Tyr Arg Arg 
        35                  40                  45              


Phe Phe Arg His Phe Pro Ala Val Lys Glu Leu Trp Leu Ile Ser Gln 
    50                  55                  60                  


Glu Leu Ser His Glu Leu Arg Ser Leu Gln Lys Asp Ile Arg Ala Leu 
65                  70                  75                  80  


Lys Ala Ser Glu Thr Lys Lys Leu Leu Gln Thr Trp Gly Gln Phe Gln 
                85                  90                  95      


Lys Ile Lys Leu Leu Thr Pro Ser Ser Leu Ala Ile Leu Gln Lys Thr 
            100                 105                 110         


Thr Thr Gln Leu Val Phe Pro Asp Glu Glu Ile Ser His His Leu Val 
        115                 120                 125             


Glu Lys Tyr Phe Ala Gln Asn Arg Val Leu Phe Ala Ser Phe Phe Leu 
    130                 135                 140                 


Arg Trp Asp Lys Lys Ser Ser Leu Lys Lys His Asp Leu Gln Glu Tyr 
145                 150                 155                 160 


Ser Glu Ile Ser Asn Lys Glu Phe Asp Gln Met Met Ile Ala Ile Ala 
                165                 170                 175     


Gln Gln Glu Ala Asp Lys Ser Asp Asp Trp Trp Arg Gln Val Gly Gly 
            180                 185                 190         


Leu Ile Phe Lys Asp Glu Thr Ile Leu Leu Leu Ala His Asn Gln His 
        195                 200                 205             


Thr Pro Thr Glu Ala Glu Ala Tyr Phe Ala Gly Asp Pro Arg Ala Asp 
    210                 215                 220                 


Phe His Gln Gly Glu Tyr Leu Lys Ile Ser Thr Ala Ile His Ala Glu 
225                 230                 235                 240 


Ala Tyr Leu Ile Ala Gln Ala Ala Lys Gln Gly Ile Ser Leu Glu Gly 
                245                 250                 255     


Ala Asp Leu Tyr Val Thr Thr Phe Pro Cys Pro Val Cys Ala Lys Gln 
            260                 265                 270         


Val Ala Tyr Ser Gly Ile Lys Arg Val Phe Phe Arg Glu Gly Tyr Ser 
        275                 280                 285             


Leu Leu Asp Gly Glu Thr Ile Leu Lys Ala Asn Gly Val Lys Leu Ile 
    290                 295                 300                 


Arg Val Thr Val 
305             


<210>  117
<211>  615
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.7; 
       tr|A0A1G3PNQ8|A0A1G3PNQ8_9SPIR polypeptide sequence

<400>  117

Met Arg Asp Leu Pro Leu Leu Val Leu Gly Leu Thr Gly Pro Met Gly 
1               5                   10                  15      


Ala Gly Cys Thr Arg Phe Ala Arg Asp Ile Ser Lys Met Glu Pro Gly 
            20                  25                  30          


Lys Val Ile Lys Lys Gln Gly Leu Leu Asp Gln Val Ala His Glu Ile 
        35                  40                  45              


Ser Glu Leu Ser Lys Lys Ala Ser Glu Ile Arg Leu Gln Cys Ile Ser 
    50                  55                  60                  


Asn Gly Lys Asn Ser Glu Leu Ala Glu Leu Lys Arg Leu Asn Arg Arg 
65                  70                  75                  80  


Leu Asn Ala Lys Leu Ala Glu Arg Ala Cys Leu His Val Ile Ala Lys 
                85                  90                  95      


Ser Ser Leu Pro Glu Pro Leu Phe Ile Ser Leu Asn Thr Ile Val Ile 
            100                 105                 110         


Lys Ile Ala Val Asp Ser Ile Thr Ala Pro Glu Phe Ala Glu Trp Ala 
        115                 120                 125             


Lys Asn His Ala Lys Val Ala Asp Leu Leu Lys Trp Leu Arg Thr Gln 
    130                 135                 140                 


Trp Glu Ser Glu Leu Thr Leu Tyr Glu Thr Trp Gly Gln Asp Ala Gly 
145                 150                 155                 160 


Arg Phe Ser Gln Asp Glu Leu Glu Lys Met Asp Ala Met Phe Ala Glu 
                165                 170                 175     


Phe Glu Arg Ile Gly Asp Glu Ile Leu Lys Glu Asp Phe Glu Thr Tyr 
            180                 185                 190         


Phe Gly Lys Arg Asn Asn Asp Phe Ser Ile Arg Met Phe Ser Glu Asn 
        195                 200                 205             


Ile Arg Leu Ser Gly Asn Pro Phe Arg Pro Ala Glu Asn Gly Gly Gly 
    210                 215                 220                 


Gly Gly Lys Tyr Asp Glu Pro Ser Met Val Met Ile Ala Arg Glu Thr 
225                 230                 235                 240 


Asp Arg Tyr Ile Arg Phe Tyr Arg Thr Arg Ser Asp Gln Lys Arg Ser 
                245                 250                 255     


His Phe Phe Ile Ile Asp Glu Ile Lys Asn Pro Arg Glu Ala Glu Tyr 
            260                 265                 270         


Phe Arg Ala Arg His Gln Asn Phe Phe Leu Val Ser Ile Phe Ser Ser 
        275                 280                 285             


Ser Glu Ile Arg Ala Ser Arg Met Arg Arg Gly Leu Gly His Asp Ala 
    290                 295                 300                 


Gly Val Ser Asp Ala Asp Phe Gln His Leu Phe Arg Glu Leu Asp Ser 
305                 310                 315                 320 


Arg Asp Trp Gly Ala Asp Asp Phe Asp Ala His Gly Leu His Arg Gln 
                325                 330                 335     


Asn Ile Tyr Arg Cys Phe Asn Leu Ala Asp Ile Ala Ile Asn Asn Asp 
            340                 345                 350         


Val Glu Asp Glu Arg Phe Ser Glu Val Leu Phe Asn Lys Phe Ile Arg 
        355                 360                 365             


Tyr Tyr Ala Leu Met Leu Ser Pro Gly Cys Val Gln Pro Thr Pro Gln 
    370                 375                 380                 


Glu Thr Tyr Met His Leu Ala Tyr Ser Leu Ser Leu Arg Ser Thr Cys 
385                 390                 395                 400 


Ile Ser Arg Gln Val Gly Ala Val Ile Thr Asp Leu Glu Asp Arg Ile 
                405                 410                 415     


Leu Ser Leu Gly Trp Asn Glu Val Pro Glu Gly Gln Ile Gly Cys Gly 
            420                 425                 430         


Leu Lys Val Lys Lys Asp Tyr Thr Asp Lys Glu Asn Pro Leu Phe Glu 
        435                 440                 445             


Met Glu Ile Trp Asp Asn Val Ile Thr Ala Glu Asp Leu Ala Val Trp 
    450                 455                 460                 


Asp Asp Glu Asp Ser Ile Cys Val Lys Asp Ile Leu Ser Arg Ile Glu 
465                 470                 475                 480 


Ile Lys Thr Lys Leu Lys Ser Val Ser Leu Thr Pro Glu Glu Arg Ala 
                485                 490                 495     


Asp Val Leu Lys Ala Leu Arg Ile Lys Arg Leu Glu Tyr Ser Arg Ser 
            500                 505                 510         


Leu His Ala Glu Glu Asn Ala Ile Leu Gln Val Ala Ser Arg Gly Gly 
        515                 520                 525             


Val Gly Leu Lys Asp Gly Thr Ile Tyr Val Thr Thr Phe Pro Cys Glu 
    530                 535                 540                 


Leu Cys Ser Lys Lys Ile Tyr Gln Val Gly Ile Ser Lys Ile Tyr Tyr 
545                 550                 555                 560 


Thr Glu Pro Tyr Pro Asn Ser Ile Ser Glu Lys Val Ile Leu Lys Asp 
                565                 570                 575     


Gly Ile Arg Asn Ile Lys Ile Leu Gln Phe Glu Gly Val Lys Ser Tyr 
            580                 585                 590         


Ser Tyr Phe Lys Leu Phe Lys Pro Gly Phe Asp Lys Lys Asp Ala Gln 
        595                 600                 605             


Met Leu Glu Gly Arg Gly Ile 
    610                 615 


<210>  118
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.8; 
       tr|A0A1G0PGF4|A0A1G0PGF4_9BACT polypeptide sequence

<400>  118

Met Lys His Asn Asn Gln Leu Arg Lys Glu Ile Glu Lys Leu Leu Gly 
1               5                   10                  15      


Gln Asn Ser Ile Ile Lys Asn Asp Glu Leu Lys Lys Leu Gln Lys Glu 
            20                  25                  30          


Tyr Lys Ile Glu Thr Asp Glu Leu Leu Ile Ser Phe Leu Pro Tyr Ala 
        35                  40                  45              


Ala Glu Phe Ala Lys Val Pro Ile Ser Lys Tyr Lys Val Gly Ala Val 
    50                  55                  60                  


Val Leu Gly Lys Ser Gly Asn Ile Tyr Phe Gly Ser Asn Met Glu Phe 
65                  70                  75                  80  


Glu Ala Gly Ala Leu Ser Ala Thr Val His Ala Glu Gln Ser Ala Val 
                85                  90                  95      


Asn Asn Ala Trp Leu Asn Gly Glu Thr Gly Ile Asn Lys Ile Ala Val 
            100                 105                 110         


Thr Ala Ala Pro Cys Gly Tyr Cys Arg Gln Phe Leu Asn Glu Leu Thr 
        115                 120                 125             


Thr Ala Lys Gln Leu His Val Leu Leu Lys Asp Lys Asn Leu Glu Ala 
    130                 135                 140                 


Ala Lys Val Phe Lys Leu Thr Glu Leu Leu Pro Glu Ala Phe Gly Pro 
145                 150                 155                 160 


Arg Asp Leu Glu Ile Glu Gly Gly Leu Met Lys Val Glu Asn His Lys 
                165                 170                 175     


Leu Lys Ile Glu Asn Ile Asn Asp Glu Leu Ile Asn Ala Ala Leu Glu 
            180                 185                 190         


Ala Ala Asn Lys Ser Tyr Ala Pro Tyr Ser Lys Asn Tyr Ser Gly Val 
        195                 200                 205             


Ser Ile Gln Leu Ser Asp Gly Thr Ile Phe Ser Gly Arg Tyr Ser Glu 
    210                 215                 220                 


Asn Ala Ala Tyr Asn Pro Ser Leu Leu Pro Phe Gln Ser Ala Leu Ala 
225                 230                 235                 240 


Phe Met Asn Met Asn Thr Lys Lys Gly Ser Asn Asn Lys Ile Val Asp 
                245                 250                 255     


Ala Val Leu Val Glu Ala Val Ser Asn Ile Ser Gln Lys Asp Ala Ala 
            260                 265                 270         


Gly Thr Leu Leu Asn Ser Ile Ser Lys Thr Lys Leu Arg Tyr Tyr Lys 
        275                 280                 285             


Ile Lys Asn 
    290     


<210>  119
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.9; 
       tr|A0A0P4WGY5|A0A0P4WGY5_9EUCA polypeptide sequence

<400>  119

Met Glu Glu Asn Ser Ser Ala Thr Ser Gln Pro Lys Cys Ala Ser Arg 
1               5                   10                  15      


Thr Lys Gln Gly Gly Asn Asp Leu Ser Thr Asp Met Ser Asn Leu Ser 
            20                  25                  30          


Val Gly Glu Thr Lys Arg Thr Asp Phe Leu Pro Trp Asp Asp Tyr Phe 
        35                  40                  45              


Met Ala Val Ala Phe Leu Ser Ala Met Arg Ser Lys Asp Pro Ser Ser 
    50                  55                  60                  


Gln Val Gly Ala Cys Ile Val Asn Ala Asp Lys Lys Ile Val Gly Ile 
65                  70                  75                  80  


Gly Tyr Asn Gly Met Pro Ile Gly Cys Ser Asp Asp Glu Leu Pro Trp 
                85                  90                  95      


Asn Lys Glu Ser Leu Asp Pro Leu Gln Thr Lys Tyr Met Tyr Val Cys 
            100                 105                 110         


His Ala Glu Met Asn Ala Ile Met Asn Lys Asn Ser Ser Asp Leu Ala 
        115                 120                 125             


Gly Cys Cys Val Tyr Val Ala Leu Phe Pro Cys Asn Glu Cys Ala Lys 
    130                 135                 140                 


Leu Val Ile Gln Ala Gly Ile Arg Glu Val Val Phe Phe Ser Asp Lys 
145                 150                 155                 160 


His Gln Gln Lys Pro Glu Thr Val Ala Ser Lys Lys Met Leu Asn Met 
                165                 170                 175     


Ala Gly Val Ala Tyr Arg Gln Tyr Thr Pro Ser Gln Ser Lys Ile Glu 
            180                 185                 190         


Leu Asn Leu Ser Leu Lys Glu Gln Glu Lys Ser Glu Pro Thr Ala Asp 
        195                 200                 205             


Ile Thr Gln Ser Ser Glu Arg Asp Gln Asn Ser Lys Arg Lys Asp Tyr 
    210                 215                 220                 


Leu Ser Trp Glu Glu Tyr Phe Met Ala Met Ala His Leu Ser Ala Leu 
225                 230                 235                 240 


Arg Ser Lys Asp Pro Ile Thr Gln Val Gly Ala Cys Ile Val Asn Ser 
                245                 250                 255     


Lys Lys Lys Ile Val Gly Ile Gly Tyr Asn Gly Met Pro Leu Gly Cys 
            260                 265                 270         


Asn Asp Asp Leu Met Pro Trp Gly Asn Ser Ser Ser Asn Lys Leu Glu 
        275                 280                 285             


Thr Lys Tyr Met Tyr Val Cys His Ala Gly Val Asn Ala Ile Met Asn 
    290                 295                 300                 


Lys Asn Ser Cys Asp Val Ser Gly Cys Thr Leu Tyr Val Ala Leu Phe 
305                 310                 315                 320 


Pro Cys Asn Glu Cys Ala Lys Val Ile Ile Gln Ala Gly Ile Lys Thr 
                325                 330                 335     


Ile Ile Tyr Ala Ser Asp Thr Asn Lys Asp Gln Ala Ser Ile Leu Ala 
            340                 345                 350         


Ser Lys Lys Met Leu Asp Met Ala Gly Ile Lys Tyr Arg Ala Asp Asn 
        355                 360                 365             


Leu Ser Gln Arg Lys Ile Val Ile Asp Phe Lys Thr Ile Asp Trp Asn 
    370                 375                 380                 


Ser Arg Phe Met Asn Asp His Gln Asn Asp Pro Thr Cys Leu 
385                 390                 395             


<210>  120
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.10; 
       tr|A0A3D8IG27|A0A3D8IG27_9HELI polypeptide sequence

<400>  120

Met Arg Lys Asn Ile Leu Tyr Phe Ile Leu Thr Leu Phe Phe Leu Ser 
1               5                   10                  15      


Gly Leu Tyr Ala Thr Ser Leu Pro Glu Asp Asn Val Val Ser Gly Val 
            20                  25                  30          


Ile Tyr Glu Lys Ile Asp Thr Val Ser Ala Glu Val Asp His Ile Tyr 
        35                  40                  45              


Pro Met Leu Ala Leu Ala Ile Val Tyr Lys Asp Trp Gln Glu Lys Asn 
    50                  55                  60                  


Met Leu Asn Lys Gln Gly His Asn Ile Gly Leu Val Ile Val Asp Glu 
65                  70                  75                  80  


Asn Asn Met Pro Val Phe Trp Val Arg Asn Ser Val His Ala Thr His 
                85                  90                  95      


Asn Gly Thr Gln His Gly Glu Val Arg Leu Val Ser Asn Leu Leu Asn 
            100                 105                 110         


Cys Glu Gly Phe Asn Lys Tyr Leu Asp Lys Tyr Thr Leu Tyr Thr Thr 
        115                 120                 125             


Leu Glu Pro Cys Ile Met Cys Ala Gly Met Leu Ser Met Val Gln Ile 
    130                 135                 140                 


Pro Lys Val Val Tyr Ala Gln Lys Asp Leu Ser Cys Gly Asn Thr Gln 
145                 150                 155                 160 


Glu Ile Ile Ser Thr Ala Lys Tyr Pro Arg Tyr Tyr Lys Ala Phe Thr 
                165                 170                 175     


Val Glu Asn Gly Tyr Lys Lys Asp Leu Glu Glu Cys Phe Glu Gln Tyr 
            180                 185                 190         


Lys Ile Cys Lys Asn Asp Ser Ile Thr Asp Phe Leu Val Asn Asp Ser 
        195                 200                 205             


Ala Lys Glu Ile Phe Arg Lys Ala Ser Asn Asp Leu Gln Asp Tyr Lys 
    210                 215                 220                 


Val Lys Phe Lys Glu Asn Arg Arg Val Ile Lys Val Ala Gln Glu Phe 
225                 230                 235                 240 


Leu Gln Asn Ile Gln Thr Lys Asp Asn Leu Asp Val Leu Gln Cys Pro 
                245                 250                 255     


Lys Asn Met 
            


<210>  121
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.11; 
       tr|A0A351C8C4|A0A351C8C4_9BACT polypeptide sequence

<400>  121

Met Asn Glu Leu Thr Lys Gln Ser Glu His Leu Arg Asn Glu Ala Leu 
1               5                   10                  15      


Arg Ile Ala Thr Arg Ser Tyr Val Pro Tyr Thr Gly Gln Gln Glu Gly 
            20                  25                  30          


Val Ile Ile Leu Leu Glu Asn Gly Asp Leu Ile Pro Gly Val Arg Val 
        35                  40                  45              


Glu Asn Ala Ser Phe Gln Leu Thr Ile Pro Ala Leu Gln Asn Ala Leu 
    50                  55                  60                  


Ser Thr Met Tyr Ala Leu Gln Arg Thr Asp Ile Ser Met Ile Val Ser 
65                  70                  75                  80  


Ser Ile Pro Phe Thr Asp Ser Asp Leu Ala Tyr Thr Gly Gly Met Ala 
                85                  90                  95      


Glu Ile Ala Trp Glu Met Val Gly Ala Ser Leu Leu Leu Val Ala Gly 
            100                 105                 110         


Ala His Ile Pro Glu Ala Gly Thr Phe Ile Asp Pro Ala Arg Gly Glu 
        115                 120                 125             


Asn Leu Leu Asp Val Ser Arg Glu Ala Ala Leu Asn Ala Phe Ile Pro 
    130                 135                 140                 


Glu Ser Asp Phe Pro Val Gly Ser Ala Ile Gln Thr Ser Asp Asp Val 
145                 150                 155                 160 


Val Ile Asp Gly Cys Asn Val Glu His Ser Asp Trp Ser Lys Ile Ile 
                165                 170                 175     


Cys Ala Glu Arg Asn Val Leu Ser Thr Ala Arg Ser Tyr Gly Leu Gly 
            180                 185                 190         


Gln Ile Thr Thr Ile Tyr Val Ser Cys Pro Lys Glu Pro Gly Gly Thr 
        195                 200                 205             


Pro Cys Gly Ala Cys Arg Gln Val Ile Val Glu Leu Ala Pro Asp Ala 
    210                 215                 220                 


Thr Val Trp Met Asp Arg Gly Asn Gln Glu Pro Ile Ala Met Lys Ala 
225                 230                 235                 240 


Thr Lys Leu Leu Pro Gly His Phe Thr Gly Asn Val Leu Lys Lys Gln 
                245                 250                 255     


<210>  122
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.12; 
       tr|A0A1G6V2K7|A0A1G6V2K7_PEPNI polypeptide sequence

<400>  122

Met Pro Ile Val Arg Val Asn Glu Ile Gly Ala Arg Leu Pro Glu Asp 
1               5                   10                  15      


Trp Glu Ala Leu Glu Thr Ala Ile Trp Gln Ala Tyr Val Ser Arg Glu 
            20                  25                  30          


Asp Leu Pro Asp Ala Gly Glu Leu Asp Leu Thr Leu Val Asp Asp Ala 
        35                  40                  45              


Thr Ile Gln Glu Leu Asn Lys Thr His Arg Gln Leu Asp Lys Ser Thr 
    50                  55                  60                  


Asp Val Leu Ser Phe Pro Met Tyr Asp Asp Arg Asp Asp Leu Ala Ala 
65                  70                  75                  80  


Asp Val Gln Ala Gly Leu Pro Val Ile Leu Gly Asp Ile Met Ile Ser 
                85                  90                  95      


Val Pro Thr Ala Glu Arg Gln Ala Gln Ala Tyr Gly His Ser Phe Lys 
            100                 105                 110         


Arg Glu Met Ala Tyr Leu Leu Val His Gly Leu Leu His Ile Ala Gly 
        115                 120                 125             


Tyr Asp His Met Ser Ala Glu Glu Lys Ser Ala Met Arg Arg Ala Glu 
    130                 135                 140                 


Glu Ala Ile Leu Ala Asp Val Asp Val Pro Arg Asp Thr Ala Pro Ser 
145                 150                 155                 160 


Lys Thr Ala Ala Val Leu Asp Glu Ala Asp Val Gln Ala Leu Ile Asp 
                165                 170                 175     


Ala Ala Arg Ala Ala Arg Leu Gln Ala Tyr Ala Pro Tyr Ser Gly Tyr 
            180                 185                 190         


Ala Val Gly Ala Ala Leu Leu Ala Ala Asp Gly Arg Arg Phe Cys Gly 
        195                 200                 205             


Val Asn Val Glu Asn Ala Ser Tyr Gly Ala Thr Cys Cys Ala Glu Arg 
    210                 215                 220                 


Thr Ala Leu Phe Ala Ala Val Thr Ala Gly Ala Arg Asp Phe Ile Ala 
225                 230                 235                 240 


Leu Ala Leu Val Thr Glu Gly Asp Glu Pro Ala Pro Pro Cys Gly Leu 
                245                 250                 255     


Cys Arg Gln Ala Leu Ala Glu Phe Ser Pro Asp Leu Ala Ile Tyr Leu 
            260                 265                 270         


Ala Gly Pro Thr Gly Glu Thr Tyr Arg Arg Thr Ser Leu Ala Ala Leu 
        275                 280                 285             


Phe Pro Glu Ala Phe Ser Leu Ser Thr Lys Glu Ser Val 
    290                 295                 300     


<210>  123
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.13; 
       tr|F2NP91|F2NP91_MARHT polypeptide sequence

<400>  123

Met Pro Val Met Glu Thr His Ala Leu Glu Ala Arg Phe Lys Glu Ala 
1               5                   10                  15      


Leu Ala Arg Leu Cys Pro Glu Gly Arg Leu Leu Ala Ala Val Ser Gly 
            20                  25                  30          


Gly Gly Asp Ser Val Ala Leu Leu Tyr Leu Leu Lys Ala Ala Gly Arg 
        35                  40                  45              


Asp Thr Ile Val Ala His Leu Asp His Ala Leu Arg Pro Asp Ser Ala 
    50                  55                  60                  


Ala Asp Ala Ala Phe Val Glu Lys Leu Ala Gln Arg Leu Gly Phe Pro 
65                  70                  75                  80  


Leu Glu Thr Glu His Val Asp Val Arg Ala Leu Ala His Arg Lys Arg 
                85                  90                  95      


Ile Asn Leu Glu Ala Ala Ala Arg Glu Val Arg Tyr Ala Phe Leu Ala 
            100                 105                 110         


Arg Val Ala Arg Arg Trp Lys Ala Arg Cys Ile Leu Thr Ala His Thr 
        115                 120                 125             


Leu Asp Asp Asn Ala Glu Thr Val Leu Leu Gln Ile Leu Arg Gly Ala 
    130                 135                 140                 


Gly Arg Gly Leu Gly Ile Arg Pro Leu Gln Arg Arg Val Ala Arg Pro 
145                 150                 155                 160 


Leu Leu Glu Phe Ser Arg Ala Glu Leu Arg Ala Tyr Leu Glu Ala Arg 
                165                 170                 175     


Gly Ala Arg Trp Leu Glu Asp Pro Thr Asn Arg Ser Leu Glu Leu Asp 
            180                 185                 190         


Arg Asn Tyr Leu Arg His Ala Val Leu Pro Arg Ile Thr Ala Arg Phe 
        195                 200                 205             


Pro His Ala Leu Glu Ala Leu Ala Arg Phe Ser Gln Ala Gln Gln Ala 
    210                 215                 220                 


Asp Asp Trp Ala Leu Glu Ala Leu Ser Ala Arg His Leu Ile Pro Asp 
225                 230                 235                 240 


Arg Arg Trp Pro Val Pro Ala Tyr Arg Ala Leu Pro Leu Glu Arg Ala 
                245                 250                 255     


Pro Glu Ala Leu Arg Arg Arg Ala Ile Arg Gly Val Leu Glu Ala Leu 
            260                 265                 270         


Gly Val Arg Pro Glu Ala Arg Leu Val Ala Asp Val Glu Ala Ala Leu 
        275                 280                 285             


Gly Gly Arg Ala Gln Thr Leu Pro Gly Gly Val Val Val Arg Arg Gln 
    290                 295                 300                 


Arg Gly Thr Leu Phe Phe Ile Pro Pro Thr Val Arg Phe Pro Lys Val 
305                 310                 315                 320 


Gln Pro Pro Ala Gly Leu Glu Ala Arg Pro Pro Arg Pro Gly Asp Tyr 
                325                 330                 335     


Leu Val Phe Pro Tyr Gly Arg Lys Arg Leu Val Asp Phe Leu Asn Glu 
            340                 345                 350         


Arg Gly Val Pro Arg Glu Leu Lys Arg Arg Trp Pro Val Gly Ala Val 
        355                 360                 365             


Gly Ala Glu Val Arg Trp Val Tyr Gly Leu Trp Pro Glu Pro Asp Glu 
    370                 375                 380                 


Asp Arg Tyr Met Arg Arg Ala Leu Val Leu Ala Arg Ala Ala Ala Arg 
385                 390                 395                 400 


Gln Gly Glu Val Pro Ile Gly Ala Val Leu Val Arg Asp Gly Ala Val 
                405                 410                 415     


Leu Ala Glu Ala Ala Asn Ala Val Glu Ala Ser Arg Asp Ala Thr Ala 
            420                 425                 430         


His Ala Glu Leu Leu Ala Leu Arg Thr Ala Leu Arg Arg Val Gly Glu 
        435                 440                 445             


Lys Val Leu Pro Gly Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Pro 
    450                 455                 460                 


Met Cys Tyr Gly Ala Ile Leu Glu Ala Arg Val Ala Arg Val Val Tyr 
465                 470                 475                 480 


Gly Val Glu Asn Leu Lys Ala Gly Ala Phe Thr Val His Gly Leu Glu 
                485                 490                 495     


Pro Arg Val Ala Leu Glu Ala Gly Arg Val Glu Gly Glu Cys Ala Lys 
            500                 505                 510         


Val Leu Lys Asp Phe Phe Ala Arg Leu Arg Pro Gly Arg Asp Gly Ala 
        515                 520                 525             


<210>  124
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.14; 
       tr|A0A316TX77|A0A316TX77_9BACT polypeptide sequence

<400>  124

Met Ile Asn Gly Tyr Thr Pro Tyr Ser Gly Asn Gln Asn Thr Cys Tyr 
1               5                   10                  15      


Val Lys Gly Glu Ser Gly Thr Phe Tyr Pro Gly Val Arg Ile Glu Asn 
            20                  25                  30          


Val Ser Tyr Pro Leu Thr Ile Ser Ser Val Gln Ala Ala Val Cys Ser 
        35                  40                  45              


Cys Leu Ala Asn Ser Asp Asn Pro Val Glu Tyr Tyr Thr Gly Asp His 
    50                  55                  60                  


Gln Pro Glu Leu Leu Gln Val Trp Ala Asp Glu Tyr Asp Met Lys Pro 
65                  70                  75                  80  


Gly Gly Lys Leu Pro Asp Ser Pro Leu Lys Leu Phe Asp Pro Leu Val 
                85                  90                  95      


Pro Ser Ile Pro Asp Ile Lys Lys Glu Leu Asp Val Leu Thr Glu Lys 
            100                 105                 110         


Ser Val Thr Pro Asn Ser Gly Phe Pro Val Ser Ala Leu Leu Gln Thr 
        115                 120                 125             


Glu Lys Gly Tyr Ile Arg Gly Val Asn Ile Glu Leu Ser Ser Trp Ala 
    130                 135                 140                 


Leu Gly Leu Cys Ala Glu Arg Val Ala Ile Ser Arg Ala Leu Thr Ala 
145                 150                 155                 160 


Gly Tyr Thr Gln Phe Lys Ser Ile His Ile Tyr Ala Pro Glu Ala Asp 
                165                 170                 175     


Phe Val Ser Pro Cys Gly Ala Cys Arg Gln Val Leu Leu Glu Val Met 
            180                 185                 190         


Pro Asp Ala Asp Thr Glu Leu Tyr His Gly Asp Gly Thr Leu Ser Lys 
        195                 200                 205             


His Ile Val Ser Asp Leu Leu Pro Phe Gly Phe Thr Ser His Lys Leu 
    210                 215                 220                 


Lys Lys 
225     


<210>  125
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.15; 
       tr|R6VYG3|R6VYG3_9FIRM polypeptide sequence

<400>  125

Met Ile His Lys Gly Thr Gln Thr Ile Glu Thr Lys Arg Leu Ile Leu 
1               5                   10                  15      


Arg Ala Phe Thr Pro Asp Asp Ala Glu Ala Ala Phe Glu Asn Trp Met 
            20                  25                  30          


Ser Asp Pro Lys Val Thr Glu Phe Leu Arg Trp Lys Thr His Ala Asp 
        35                  40                  45              


Ile Ser Asp Ser Arg Lys Ile Val Asn Glu Trp Ala Asn Gly Ser Ala 
    50                  55                  60                  


Asp Pro Glu Phe Tyr Gln Trp Ala Ile Val Pro Lys Asp Val Asn Glu 
65                  70                  75                  80  


Pro Ile Gly Thr Ile Ser Val Val Asp Arg Asn Asp Ala Leu Gly Ile 
                85                  90                  95      


Phe His Ile Gly Tyr Cys Ile Gly Ser Lys Trp Trp His Lys Gly Ile 
            100                 105                 110         


Thr Ser Glu Ala Phe Ser Ala Val Ile His Phe Leu Phe Glu Glu Val 
        115                 120                 125             


Gly Ala Asn Arg Ile Glu Ser Gln His Asp Pro Glu Asn Ile His Ser 
    130                 135                 140                 


Gly Asp Val Met Lys Lys Cys Gly Leu Thr Phe Glu Gly Thr Leu Arg 
145                 150                 155                 160 


Gln Ala Asp Phe Asn Asn Arg Gly Ile Val Asp Ala Cys Val Tyr Ser 
                165                 170                 175     


Ile Leu Gln Ser Glu Trp Gln Asn Asn Thr Ser Val Trp Gln Arg Leu 
            180                 185                 190         


Tyr Asn Ala Ala Leu Thr Val Gln Asn Asp Arg Val Val Ser Pro Phe 
        195                 200                 205             


Ile Asp Ala Gly Gly Val Ala Ala Ala Leu Met Thr Lys Lys Gly Asn 
    210                 215                 220                 


Ile Tyr Thr Gly Ile Cys Ile Asp Thr Ala Ser Thr Leu Gly Met Cys 
225                 230                 235                 240 


Ala Glu Arg Asn Ala Val Ala Asn Met Leu Thr Asn Gly Glu Ser Arg 
                245                 250                 255     


Ile Asp Lys Ile Val Ala Val Met Pro Asp Gly Lys Val Gly Ala Pro 
            260                 265                 270         


Cys Gly Ala Cys Arg Glu Tyr Met Met Gln Leu Asp Arg Asp Ser Gly 
        275                 280                 285             


Asp Ile Glu Ile Leu Leu Asp Leu Glu Thr Glu Lys Thr Val Arg Leu 
    290                 295                 300                 


Lys Asp Leu Ile Pro Asp Trp Trp Gly Ala Glu Arg Phe Gly Asp Thr 
305                 310                 315                 320 


Glu 
    


<210>  126
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.16; 
       tr|A0A3C1HZ18|A0A3C1HZ18_9BACI polypeptide sequence

<400>  126

Met Gly Asp Ile Met Glu Asn Trp Asn Glu Leu Ser Glu Pro Trp Lys 
1               5                   10                  15      


Arg Cys Phe Leu Gln Ala Trp Lys Ala Tyr Cys His Gly Ser Ile Pro 
            20                  25                  30          


Ile Gly Ala Val Leu Val Asp Ser Glu Gly Glu Ile Phe Leu Glu Gly 
        35                  40                  45              


Arg Asn Arg Val His Glu Leu Thr Ala Pro Glu Gly Gln Leu Cys Asp 
    50                  55                  60                  


Cys Arg Ile Ala His Ala Glu Met Asn Val Leu Val Gln Val Lys Thr 
65                  70                  75                  80  


Ser Asp Tyr Glu Lys Leu Ser Gly Ala Thr Ile Tyr Ser Thr Met Glu 
                85                  90                  95      


Pro Cys Ile Gln Cys Phe Gly Ala Ile Ile Leu Ser Arg Ile Lys Asn 
            100                 105                 110         


Ile Ser Phe Ala Ala Ile Asp Asp Lys Leu Ala Gly Ala Thr Thr Leu 
        115                 120                 125             


Glu Asp Arg His Gly Phe Ile Lys Ser Arg Asn Leu Asn Ile Ala Gly 
    130                 135                 140                 


Pro Phe Ser His Leu Gly Glu Ile Gln Ile Ile Leu Arg Thr Asp Phe 
145                 150                 155                 160 


Leu Leu Arg Ile Phe Asp Ser Glu Tyr Ala Asp Pro Leu Ile Ala Ala 
                165                 170                 175     


His Glu Lys Asp Tyr Pro Ile Gly Val Ala Leu Gly Arg His Tyr His 
            180                 185                 190         


Arg Asn Asn Arg Leu Gln Val Ala Lys Lys Glu Thr Ile Pro Phe Gly 
        195                 200                 205             


Glu Leu Phe Asn Glu Phe Ser Phe Asp Ile Lys Arg Ala Arg Glu Gly 
    210                 215                 220                 


Tyr Thr Leu Gly Lys 
225                 


<210>  127
<211>  205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.17; 
       tr|A0A1M6KV24|A0A1M6KV24_9BACT polypeptide sequence

<400>  127

Met Glu Ala Ser Gln Gln Asn Ile Leu Leu Lys Ile Glu Gly Lys Gly 
1               5                   10                  15      


Pro Val Ala Glu Ile Asn Phe Thr Val Thr Leu Pro Glu Trp Leu Val 
            20                  25                  30          


Glu Gln Val Gln Ser Gly Ser Thr Val Phe Leu Thr Gln Lys Glu Lys 
        35                  40                  45              


Met Arg Phe Val Leu Glu Leu Ala Arg Lys Asn Val Ala Gln Glu Thr 
    50                  55                  60                  


Gly Gly Pro Phe Ala Ala Ala Val Phe Ser Leu Glu Ser Gly Glu Leu 
65                  70                  75                  80  


Val Ser Ala Gly Val Asn Val Val Val Glu Ser Arg Cys Ser Ser Ala 
                85                  90                  95      


His Ala Glu Val Val Ala Leu Ser Leu Ala Gln Lys Ala Val Asp Ser 
            100                 105                 110         


His Asp Leu Gly Ala Ala Gly Leu Pro Arg Met Val Leu Val Ser Ser 
        115                 120                 125             


Ala Glu Pro Cys Ala Met Cys Met Gly Ala Ile Pro Trp Ser Gly Val 
    130                 135                 140                 


Lys Gln Val Ile Cys Gly Ala Arg Asp Glu Asp Val Arg Ser Val Gly 
145                 150                 155                 160 


Phe Asp Glu Gly Ala Lys Pro Leu Glu Trp Val Glu Asp Phe Ala Glu 
                165                 170                 175     


Arg Gly Ile Glu Val Ile Arg Asp Val Leu Arg Glu Glu Ala Thr Glu 
            180                 185                 190         


Val Leu Trp Asp Tyr Arg Glu Arg Gly Gly Glu Ile Tyr 
        195                 200                 205 


<210>  128
<211>  207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.18; 
       tr|A0A2U0T9B4|A0A2U0T9B4_9RHIZ polypeptide sequence

<400>  128

Met Glu Thr Ala Glu Leu Ile Ser Arg Leu Leu Asp Val Ile Glu Lys 
1               5                   10                  15      


Asp Ile Ala Pro Val Thr Ala Lys Gly Val Ala Arg Gly Asn Lys Leu 
            20                  25                  30          


Phe Gly Ala Ala Ile Leu Lys Lys Ser Asp Leu Ala Val Ile Val Ala 
        35                  40                  45              


Glu Thr Asn Asn Glu Ile Glu Asn Pro Leu Trp His Gly Glu Met Gln 
    50                  55                  60                  


Ala Ile Lys Arg Phe Phe Glu Leu Pro Ala Asp Gln Arg Pro Ala Thr 
65                  70                  75                  80  


Arg Asp Cys Leu Phe Leu Ala Thr His Glu Pro Cys Ser Leu Cys Leu 
                85                  90                  95      


Ser Gly Ile Thr Trp Ser Gly Phe Asp Asn Phe Tyr Tyr Leu Phe Ser 
            100                 105                 110         


His Gln Asp Ser Arg Asp Gly Phe Ala Ile Pro Tyr Asp Ile Gln Ile 
        115                 120                 125             


Leu Lys Ser Val Tyr Ala Val Pro Glu Pro Glu Thr Gly Thr Val Ser 
    130                 135                 140                 


Pro Ala Arg Asp Leu Tyr Asn Arg Ser Asn Asp Phe Trp Thr Ser His 
145                 150                 155                 160 


Gly Leu Gln Asp Met Ile Ala Gly Leu Ala Arg Ser Asn Arg Glu Ala 
                165                 170                 175     


Leu Leu Ala Arg Ile Asp Asp Leu Asn Ala Leu Tyr Ala Glu Leu Ser 
            180                 185                 190         


Glu Arg Tyr Gln Arg Asp Lys Gly Gly Lys Gly Ile Pro Leu Pro 
        195                 200                 205         


<210>  129
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.19; 
       tr|A0A2K9PN08|A0A2K9PN08_9FLAO polypeptide sequence

<400>  129

Met Ser Asp Lys Lys Glu Ser Lys Ile Lys Ile Ser Lys Thr Ser Glu 
1               5                   10                  15      


Ser Ile Glu Leu Asp Glu Ile His Ser Leu Leu Ser Tyr Ser Ile Val 
            20                  25                  30          


Gln Lys Phe Trp Glu Asn Asp Asp Arg Asn Gly Arg Gly Tyr Asn Val 
        35                  40                  45              


Gly Val Ile Leu Val Asp Glu Asn Lys Asn Ile Val Asp Trp Asp Ile 
    50                  55                  60                  


Asn Ser Val Asn Lys Thr Glu Asn Ser Thr Gln His Gly Glu Met Arg 
65                  70                  75                  80  


Leu Ile Ser Arg Tyr Leu Asp Lys Asp Glu Leu Tyr Ser Leu Lys Gly 
                85                  90                  95      


Tyr Thr Met Tyr Pro Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Met 
            100                 105                 110         


Met Thr Met Thr Asn Val Tyr Arg Thr Val Asn Gly Gln Met Asp Tyr 
        115                 120                 125             


Phe Tyr Ser Lys Ala Leu Glu Arg Leu Ser Ile Asp Thr Arg Glu Cys 
    130                 135                 140                 


Gly Gly Tyr Pro Pro Tyr Pro Arg Thr Val Ile Ser Glu Ile Ser Pro 
145                 150                 155                 160 


Ser Ser Ile Ser Thr Arg Leu Asp Ala Glu Tyr Lys Gln Tyr Thr Asn 
                165                 170                 175     


Ala Gly Asn Lys Pro Ile Ile Thr Lys Phe Leu Ser Thr Tyr Lys Ala 
            180                 185                 190         


Lys Thr Ile Tyr Asp Asp Ala Phe Asn Gln Phe Ile Asn Phe Lys Cys 
        195                 200                 205             


Lys Phe Pro Glu Asn Lys Thr Lys Tyr Glu Asn Ala Ile Lys Phe Tyr 
    210                 215                 220                 


Asn Ser Leu Pro Glu Ser Ile 
225                 230     


<210>  130
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.20; 
       tr|F4PWM7|F4PWM7_CAVFA polypeptide sequence

<400>  130

Met Arg Phe Ser Leu Ser Leu Leu Phe Val Ile Leu Ser Val Leu Leu 
1               5                   10                  15      


Ala Gly Val Leu Ala Cys Lys Asp Pro Tyr Asn Pro Glu Thr Val Asp 
            20                  25                  30          


Tyr Gly Gln Cys Ala Ser Ala Thr Lys Ala Asn Tyr Glu Val Arg Ser 
        35                  40                  45              


Asp Ser Lys Val Leu Thr Pro Ala Asp Leu Pro Ala Asp Glu Leu Ala 
    50                  55                  60                  


Val His Glu Ser Arg Met Arg His Ile Ile Asp Ile Ala Arg Val Asn 
65                  70                  75                  80  


Asn Lys Lys Phe Val Ser Ser Ile Tyr Phe Pro Asn Gly Thr Leu Ala 
                85                  90                  95      


Cys Ile Gly Ile Asn Thr Gly Lys Pro Asn Met Ile Ala His Gly Glu 
            100                 105                 110         


Ile Val Ala Ile Gln Asn Cys Thr Glu Ile His Gly Ile Ser Met Tyr 
        115                 120                 125             


Thr Asn Tyr Ser Ile Tyr Thr Thr Gly Glu Pro Cys Ser Met Cys Ala 
    130                 135                 140                 


Ser Ala Ile Leu Trp Ser Arg Phe Lys Thr Val Val Trp Ser Thr Tyr 
145                 150                 155                 160 


Asn Ser Asp Leu Tyr Cys Lys Ile Cys Met Ser Asn Ile Pro Ile Asp 
                165                 170                 175     


Ser Ser Tyr Ile Phe Ser Arg Ala Tyr Gly Leu Gly Ile Glu Ala Pro 
            180                 185                 190         


Val Ala Ile Gly Gly Val Val Lys Ala Glu Gly Asp Ala Trp Phe Gly 
        195                 200                 205             


Thr Tyr Cys Asn Arg Pro Thr Ser Ile Tyr Tyr Ile Ala Pro Lys Cys 
    210                 215                 220                 


Ala Cys Gln Asp Pro Ala Lys Val Ser Pro Leu Lys Phe Thr Gln Thr 
225                 230                 235                 240 


Arg Thr Thr Val Trp Val Glu Gly Gly Asp Lys Val Val Thr Gln Trp 
                245                 250                 255     


Asn Ala Ile Ile Ser Asn Pro Ser Asn Ser Thr Ile Val Asp Pro Pro 
            260                 265                 270         


Ile Val Ile Ser Pro Ser Val Val Phe Lys Gly Ala Pro Trp Gly Ile 
        275                 280                 285             


Ser Ala Ala Ser Glu Pro Asn Thr Tyr Lys Leu Ser Tyr Asn Lys Val 
    290                 295                 300                 


Leu Phe Pro Gly Gln Thr Phe Ser Phe Gly Tyr Ser Val Tyr Gly Leu 
305                 310                 315                 320 


Glu Glu Val Ala Phe Thr Ala Leu Glu Ala 
                325                 330 


<210>  131
<211>  193
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.21; 
       tr|U7QZM1|U7QZM1_PHOTE polypeptide sequence

<400>  131

Met Asn Lys Thr Arg Arg Lys Leu Leu Ala Thr Leu Gly Ile Met Ser 
1               5                   10                  15      


Ile Ser Met Ser Phe Ile Ala Gln Ala Gly Glu Lys Lys Thr Gln Val 
            20                  25                  30          


Ile Asn Asn Ile Leu Ser Lys Gln Glu Ile Thr Glu His Glu Lys Tyr 
        35                  40                  45              


Met Arg Glu Ala Ile Lys Glu Ala Ile Lys Asn Pro Lys His Pro Phe 
    50                  55                  60                  


Gly Ala Val Ile Val Asn Arg Asn Asn Gly Glu Ile Leu Ser Arg Gly 
65                  70                  75                  80  


Val Asn Thr Gly Arg Asn Asn Pro Ile Leu His Gly Glu Ile Gln Ala 
                85                  90                  95      


Ile Asn His Tyr Ile Thr Gln Tyr Gly Asn Gln Gly Trp Glu Asn Val 
            100                 105                 110         


Ala Leu Tyr Thr Thr Gly Glu Pro Cys Ser Met Cys Met Ser Ala Leu 
        115                 120                 125             


Val Trp Ile Gly Ile Arg Glu Val Ile Trp Ala Thr Ser Ile Ser Val 
    130                 135                 140                 


Ile Arg Asn Ser Gly Ile Arg Gln Ile Asp Ile Ser Ala His Glu Ile 
145                 150                 155                 160 


Ala Glu Arg Ala Ser Ser Phe Tyr Asn Pro Ile Thr Leu Val Gly Gly 
                165                 170                 175     


Ile Leu Ala Asn Glu Thr Asp Lys Leu Phe Leu Glu Arg Lys Arg Gly 
            180                 185                 190         


Asn 
    


<210>  132
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.22; 
       tr|A0A081CH48|A0A081CH48_PSEA2 polypeptide sequence

<400>  132

Met Ala Ser Arg Arg His Leu Leu Ala Thr Gln Val Thr Gly Asn His 
1               5                   10                  15      


Arg Lys Leu Ser Leu Trp His Leu Arg Gly Trp Leu Ser Pro Tyr Thr 
            20                  25                  30          


Lys Leu Val Asp Ala Val Tyr Phe Leu Thr Thr Asn Ser Phe Tyr His 
        35                  40                  45              


Ser Leu Gln Thr Pro Pro Val Gln Ser Ile Thr Met Leu Leu Ser Ser 
    50                  55                  60                  


Ile Ile Thr Ser Leu Ala Leu Ala Ala Gln Ala Ser Ala Tyr Arg Glu 
65                  70                  75                  80  


Gly Leu His Pro Glu Phe Gln Ser Gly Leu Ser Ile Asn Ser Val Pro 
                85                  90                  95      


Ala Thr Asp Arg Asp His Trp Met Arg Leu Ala Asn Ser Ala Ile Tyr 
            100                 105                 110         


Tyr Pro Pro Val Ser His Pro Cys Pro Gln Ala Pro Phe Gly Thr Ala 
        115                 120                 125             


Ile Val Asn Thr Thr Ser Asn Glu Leu Ile Cys Ala Ile Ala Asn Arg 
    130                 135                 140                 


Val Gly Ser Thr Gly Asp Pro Thr Gln His Gly Glu Ile Thr Ala Ile 
145                 150                 155                 160 


Gln His Cys Thr Asn Val Met Arg Lys Lys Gly Leu Ser Pro Gln Glu 
                165                 170                 175     


Ile Ile Ala Ala Trp Lys Gln Leu Ser Leu Tyr Thr Asn Ala Glu Pro 
            180                 185                 190         


Cys Thr Met Cys Leu Ser Ala Ile Arg Trp Ala Gly Phe Lys Glu Val 
        195                 200                 205             


Ile Tyr Gly Thr Ser Val Gly Thr Ile Ser Glu Asn Gly Arg Asn Gln 
    210                 215                 220                 


Ile Tyr Ile Pro Ser Asn Leu Val Leu Glu Lys Ser Tyr Ser Phe Gly 
225                 230                 235                 240 


His Ala Thr Leu Met Leu Gly Asn Ile Leu Thr His Glu Thr Asp Pro 
                245                 250                 255     


Phe Phe Gln His Gln Phe Asn Glu Ser Ala Pro Cys Pro Val Gly Cys 
            260                 265                 270         


Glu Arg Thr Gln Val Gly Glu Ala Arg Val Lys Thr Cys Glu Pro Val 
        275                 280                 285             


Pro Asn Trp Gln Lys Leu Val Arg Leu Glu Tyr Ser Glu Asp Ser Arg 
    290                 295                 300                 


Val Gly Ser Glu Pro Val Ala His Thr Pro Leu His Leu Glu Leu 
305                 310                 315                 


<210>  133
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.23; 
       tr|A0A3D3HMU1|A0A3D3HMU1_9GAMM polypeptide sequence

<400>  133

Met Asp Tyr Ser Asp Ala Ile Leu Gly Ala Ile Thr Ser Ile Arg Arg 
1               5                   10                  15      


Asn Ser Lys Gln Pro Gly Val Asn Val Thr Asp Asn Val Thr Asp Ser 
            20                  25                  30          


Ser Thr Gln Tyr Asn Asn Asp Glu Tyr Trp Met Arg Arg Ala Leu Ala 
        35                  40                  45              


Leu Ala Arg Glu Ala Gly Glu Ala Gly Glu Ile Pro Val Gly Ala Val 
    50                  55                  60                  


Leu Val Lys Asp Asn Gln Gln Val Ala Gly Gly Phe Asn Gln Pro Ile 
65                  70                  75                  80  


Arg Ser His Asp Pro Ala Ala His Ala Glu Ile Leu Thr Leu Arg Glu 
                85                  90                  95      


Ala Gly Ala Val Leu Gly Asn Tyr Arg Leu Ile Asp Thr Thr Leu Tyr 
            100                 105                 110         


Val Thr Leu Glu Pro Cys Met Met Cys Ala Gly Ala Leu Val His Ser 
        115                 120                 125             


Arg Ile Lys Arg Leu Val Phe Gly Ala Ala Glu Pro Lys Thr Gly Ala 
    130                 135                 140                 


Ala Gly Ser Phe Ile Asp Leu Leu Thr Leu Pro Arg Leu Asn His Tyr 
145                 150                 155                 160 


Met Glu Val Thr Gly Gly Val Leu Gly Glu Glu Cys Ser Val Leu Leu 
                165                 170                 175     


Ser Asp Phe Phe Arg Arg Arg Arg Ala Glu Lys Lys Ala Leu Lys Arg 
            180                 185                 190         


Gln Asn Ser Glu Ser Gly Ser Asp Ser Ala Ser 
        195                 200             


<210>  134
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.24; 
       tr|A0A1N5WT13|A0A1N5WT13_9ACTN polypeptide sequence

<400>  134

Met Leu Glu Lys Ile Glu Arg Arg Leu Val Ala Ala Ala Glu Ala Val 
1               5                   10                  15      


Val Arg Ser Pro Ser Thr Gly Asp Ala His Thr Val Ala Ala Ala Ala 
            20                  25                  30          


Met Asp Ala Asn Gly Asp Ile Tyr Ser Gly Val Asn Val Phe His Phe 
        35                  40                  45              


Thr Gly Gly Pro Cys Ala Glu Leu Val Val Ile Gly Ser Ala Ala Ala 
    50                  55                  60                  


Ala Asn Ala Pro Pro Leu Ile Thr Ile Val Ala Val Gly Asp Gly Asp 
65                  70                  75                  80  


Arg Gly Val Ile Ala Pro Cys Gly Arg Cys Arg Gln Val Met Leu Asp 
                85                  90                  95      


Leu His Pro Asp Val Phe Val Ile Val Pro Thr Gly Asp Gly Gln Leu 
            100                 105                 110         


Ala Ala Lys Pro Val Arg Glu Leu Leu Pro Phe Gly Tyr Val Ala Arg 
        115                 120                 125             


Thr Gly Ser Thr Ala Pro Arg Val Val Tyr Phe His Pro Arg His Tyr 
    130                 135                 140                 


Asp Thr Ile Ser Ser Gly Leu Lys Thr Ala Thr Val Arg Phe Gln Asp 
145                 150                 155                 160 


Ser Val Gln Thr Gly Pro Ala Val Phe Val Phe Asp Asp Gly Glu Ser 
                165                 170                 175     


Ile Arg Arg Leu Asp Ala Val Val Glu Lys Val Glu Ser Arg Arg Leu 
            180                 185                 190         


Asp His Leu Thr Glu Glu Asp Ala His His Glu Ala Leu Pro Asp Ser 
        195                 200                 205             


Asp Ala Leu Arg Asp Ala Ile Lys Thr Gln Tyr Pro Met Leu Gly Asp 
    210                 215                 220                 


Gly Asp Val Val Asp Val Ala Thr Phe Arg Leu Thr Ala Ile Ser Ala 
225                 230                 235                 240 


Pro Asp Pro Asp Pro Arg Ser Ser Tyr Pro Pro Ala Val Ser Arg Cys 
                245                 250                 255     


Asn Pro Ala Gly Pro Arg Ala Asp Leu Leu Val Gly Gln Ser 
            260                 265                 270 


<210>  135
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.25; 
       tr|X0SAC5|X0SAC5_9ZZZZ polypeptide sequence

<400>  135

Met Thr Lys Asp Gly Arg Val Ile Ala Ser Ala His Asp Thr Glu Val 
1               5                   10                  15      


Thr Asp Gln Asp Ser Thr Ala His Ala Glu Ile Asn Ala Ile Arg Lys 
            20                  25                  30          


Ala Ser Lys Ile Tyr Arg Lys Asp Leu Thr Gly Cys Leu Ile Ile Ser 
        35                  40                  45              


Thr His Glu Pro Cys Pro Met Cys Thr Gly Ser Ile Ile Trp Ser Asn 
    50                  55                  60                  


Ile Ser Lys Val Val Tyr Gly Val Ser Ile Arg Asp Ser Ile Lys Ala 
65                  70                  75                  80  


Gly Arg Asp Met Ile Asn Leu Ser Cys Lys Glu Ile Ile Lys Lys Pro 
                85                  90                  95      


Asn Ala Glu Ile Asn Ile Tyr Asp Gly Ile Leu Lys Lys Glu Cys Leu 
            100                 105                 110         


Lys Leu Tyr Asn Asn Asp Thr Arg Lys Leu Val Lys Lys Phe Arg Lys 
        115                 120                 125             


Tyr Glu Trp Ile Asn Ile Glu Glu Asn Leu Leu Asn Lys Arg Met Gln 
    130                 135                 140                 


Trp Phe Glu Asn Asn Lys Thr Met Ile Arg Lys Leu Lys Gly Asn Asp 
145                 150                 155                 160 


Leu Glu Lys Ala Tyr His Leu Ile Leu Met Lys Ile Gly Ile Lys Arg 
                165                 170                 175     


Ser Glu Ala Pro Ile Val Lys Lys Ser Glu Ser Lys Ile Ile Phe His 
            180                 185                 190         


Ser Lys Asn Tyr Cys Pro Ser Leu Glu Ala Cys Ile Ile Leu Asp Leu 
        195                 200                 205             


Asp Thr Arg Glu Val Cys Lys Glu Ile Tyr Glu Arg Pro Thr Glu Glu 
    210                 215                 220                 


Leu Ile Arg Arg Leu Asn Ser Lys Leu Arg Phe Thr Arg Asn Tyr Asp 
225                 230                 235                 240 


Cys Ile Arg Pro Tyr Ser Asp Tyr Cys Glu Glu Ile Ile Ile Leu Glu 
                245                 250                 255     


Lys 
    


<210>  136
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.26; 
       tr|A0A3B8IC10|A0A3B8IC10_9BACT polypeptide sequence

<400>  136

Met Pro Ser His Glu Asp Phe Ile His Gln Cys Leu Glu Leu Gly Lys 
1               5                   10                  15      


Glu Ala Leu Leu Gln Gly Asn Pro Pro Val Gly Ser Val Ile Val Trp 
            20                  25                  30          


Gln Asp Gln Val Ile Gly Arg Gly Ile Glu Asn Gly Arg Ser Ser Gly 
        35                  40                  45              


Asp Ile Thr Gln His Ala Glu Leu Leu Ala Leu Gln Glu Ala Val Ala 
    50                  55                  60                  


Thr Gly Gln Arg Asp Lys Leu Lys Glu Ala Ile Ile Tyr Ser Thr His 
65                  70                  75                  80  


Glu Pro Cys Val Met Cys Ala Tyr Pro Ile Arg Gln Tyr Lys Ile Pro 
                85                  90                  95      


Thr Val Val Tyr Ser Val Ala Val Pro Glu Leu Gly Gly His Thr Ser 
            100                 105                 110         


Ser Trp His Leu Leu Thr Thr Glu Asp Val Pro Lys Trp Gly Lys Ala 
        115                 120                 125             


Pro Lys Ile Ile Thr Gly Ile Ser Ala Glu Glu Val Glu Ala Leu Asn 
    130                 135                 140                 


Ala Ala Phe Gln Asp Ser Leu Lys Lys Gly 
145                 150                 


<210>  137
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.27; 
       tr|A0A2N9P8B9|A0A2N9P8B9_9FLAO polypeptide sequence

<400>  137

Met Phe Ile Phe Lys Leu Ile Ser Pro Pro Val Ser Ile Glu Val Tyr 
1               5                   10                  15      


Gln Asp Lys Ile Ile Gln Lys Leu Tyr Ile Cys Phe Met Glu Asn Ile 
            20                  25                  30          


Phe Thr Asp Glu Tyr Phe Met Lys Lys Ala Leu Gln Glu Ala Glu Thr 
        35                  40                  45              


Ala Phe Gln Gln Gly Glu Ile Pro Val Gly Ala Val Ile Val Ile Asp 
    50                  55                  60                  


Asn Arg Ile Ile Ala Arg Ser His Asn Leu Thr Glu Met Leu Asn Asp 
65                  70                  75                  80  


Val Thr Ala His Ala Glu Met Gln Ala Ile Thr Ala Ser Ala Asn Phe 
                85                  90                  95      


Leu Gly Gly Lys Tyr Leu Lys Asp Cys Thr Leu Tyr Val Thr Leu Glu 
            100                 105                 110         


Pro Cys Gln Met Cys Ala Gly Ala Leu Tyr Trp Ser Gln Ile Ser Lys 
        115                 120                 125             


Ile Val Tyr Gly Ala Thr Asp Glu Gln Arg Gly Tyr Arg Ala Met Gly 
    130                 135                 140                 


Ala Gln Leu His Pro Lys Thr Lys Val Ile Ser Gly Ile Met Gln Asn 
145                 150                 155                 160 


Glu Cys Thr His Leu Met Lys Asp Phe Phe Lys Gln Arg Arg Ser Lys 
                165                 170                 175     


Ser Thr Lys Asp 
            180 


<210>  138
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.28; 
       tr|K1KX30|K1KX30_9BACT polypeptide sequence

<400>  138

Met Val Lys Asn Pro Val Asn Asn Asn Glu Leu Tyr Phe Gly Lys His 
1               5                   10                  15      


Ser Glu Ile Pro Met Asn Glu Glu Gln Lys Ala Tyr Met Lys Met Ala 
            20                  25                  30          


Val Asp Leu Ser Arg Ser Gly Met Glu Ser Gly Lys Gly Gly Pro Phe 
        35                  40                  45              


Gly Cys Val Ile Val Lys Asp Gly Lys Val Ile Gly Ile Gly Ser Asn 
    50                  55                  60                  


Ser Val Leu Glu Thr Asn Asp Pro Thr Ala His Ala Glu Ile Val Ala 
65                  70                  75                  80  


Ile Arg Asp Ala Cys Arg Asn Leu Gly His Phe Gln Leu Asp Gly Cys 
                85                  90                  95      


Glu Val Tyr Thr Ser Cys Glu Pro Cys Pro Met Cys Leu Gly Ala Ile 
            100                 105                 110         


Tyr Trp Ala Arg Pro Ser Lys Val Phe Phe Ala Asn Asp Lys Arg Asp 
        115                 120                 125             


Ala Ala Glu Ala Gly Phe Asp Asp Asp Phe Ile Tyr Gln Glu Leu Glu 
    130                 135                 140                 


Leu Pro Tyr Glu Lys Arg Lys Ile Pro Phe Glu Gln Gly Met Gln Asp 
145                 150                 155                 160 


Thr Ala Lys Glu Val Phe Gln Glu Trp Ile Leu Lys Glu Asp Lys Thr 
                165                 170                 175     


Leu Tyr 
        


<210>  139
<211>  196
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.29; 
       tr|R4XI84|R4XI84_TAPDE polypeptide sequence

<400>  139

Met Ser Ser Glu Ile Glu Pro Pro Ser Thr Asp Val His Lys His Ala 
1               5                   10                  15      


Val Ala Glu Ala Ala Asp Glu Ser Gly Ala Ala Asp Ala Phe Met Gln 
            20                  25                  30          


Ile Ala Leu Gln Gln Ala Glu Thr Ala Leu Leu Asn Lys Glu Val Pro 
        35                  40                  45              


Val Gly Cys Val Phe Val His Gln Pro Thr Gly Thr Val Leu Ala Thr 
    50                  55                  60                  


Gly Ala Asn Gln Thr Asn Ala Ser Leu Asn Gly Thr Leu His Ala Glu 
65                  70                  75                  80  


Phe Val Ala Ile Glu Ser Ile Leu Arg Asp His Pro Pro Ser Ile Phe 
                85                  90                  95      


Arg Glu Ser Asp Leu Tyr Val Thr Val Glu Pro Cys Val Met Cys Ala 
            100                 105                 110         


Ser Ala Leu Arg Gln Leu Gln Val Arg Lys Val Tyr Phe Gly Cys Gly 
        115                 120                 125             


Asn Asp Arg Phe Gly Gly Cys Gly Ser Val Phe Ser Ile His Ser Asp 
    130                 135                 140                 


Ala Ser Lys Thr Gly Asp Ala Ala Tyr Met Val Glu Ser Gly Ile Phe 
145                 150                 155                 160 


Arg Lys Glu Ala Ile Met Leu Leu Arg Arg Phe Tyr Leu Leu Gln Asn 
                165                 170                 175     


Glu Ser Ala Pro Lys Pro Ala Leu Lys Ser Thr Arg Val Leu Lys Glu 
            180                 185                 190         


His Phe Asp Glu 
        195     


<210>  140
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.30; 
       tr|A0A239CVF7|A0A239CVF7_9DELT polypeptide sequence

<400>  140

Met Ser Pro Ala Ser Lys Lys His Phe Pro Ser Leu Phe Ser Phe Leu 
1               5                   10                  15      


Leu Leu Thr Ile Gly Leu Ile Cys Gly Thr Ala His Ala Gln Pro Gln 
            20                  25                  30          


Gly His Thr Ala Asp Asp Thr Ala Ala Thr Leu Ala Asn Ala Ser Leu 
        35                  40                  45              


Lys Glu His Glu Pro Phe Ile Arg Arg Cys Tyr Gln Leu Ala Ile Asp 
    50                  55                  60                  


Ala Gly Lys Lys Gly Asn His Pro Phe Gly Ala Leu Leu Val His Lys 
65                  70                  75                  80  


Gly Lys Ile Val Leu Glu Ala Glu Asn Thr Val Leu Thr Asp Asn Asp 
                85                  90                  95      


Phe Thr Asn His Ala Glu Met Asn Leu Ile Ala Glu Ala Ala Arg Thr 
            100                 105                 110         


Leu Ser Arg Gln Ile Ile Pro Glu Ala Thr Val Tyr Thr Ser Cys Ala 
        115                 120                 125             


Pro Cys Ala Met Cys Thr Ala Thr Leu Ala Met Ala Gly Phe Thr Arg 
    130                 135                 140                 


Ile Val Tyr Gly Val Ser His Asp Ala Leu Asn Lys Arg Phe Gly Leu 
145                 150                 155                 160 


Lys Gly Lys Ser Val Ser Cys Pro Ala Leu Phe Lys Thr Met Gly Met 
                165                 170                 175     


Glu Leu Glu Phe Val Gly Pro Val Leu Glu Lys Glu Gly Leu Arg Val 
            180                 185                 190         


Phe Asp Phe Trp Pro Glu Lys Asp Pro His Ala Gln Met Leu Lys Lys 
        195                 200                 205             


Gln Ala Arg Lys 
    210         


<210>  141
<211>  320
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.31; 
       tr|A0A1Q3NME1|A0A1Q3NME1_9BACT polypeptide sequence

<400>  141

Met Thr Glu Phe Asn Tyr Asp Trp Ala Lys Leu Ala Phe Ser Ser Lys 
1               5                   10                  15      


Arg Pro Leu Thr Asn Leu Lys Ala Thr Phe Ile Ile Ala Pro Arg Glu 
            20                  25                  30          


Ile Ser Glu Lys Arg Phe Thr Gln Leu Leu Lys Glu Tyr Leu Pro Lys 
        35                  40                  45              


Gly Asp Ile Leu Leu Gly Ile Ser Lys Glu Asp Tyr Val Glu Gly Leu 
    50                  55                  60                  


Glu Gly Gln Pro Gln Phe Ala Met Leu Gln Gln Lys Thr Leu Gln Lys 
65                  70                  75                  80  


Leu Ile Asp Lys Val Asn Asp Ala Ser Ala His Lys Val Tyr Thr Leu 
                85                  90                  95      


Arg Tyr Phe Gln Arg Glu Leu Pro Ala Ile Ile Glu Lys Leu Thr Pro 
            100                 105                 110         


Pro Arg Val Val Gly Ile His Gly Ser Trp His His Ser Phe His Thr 
        115                 120                 125             


Leu Pro Ile Tyr Tyr Leu Leu Ser Glu Lys Arg Ile Pro Tyr Gln Leu 
    130                 135                 140                 


Val Ala Ala Phe Ser Asp Glu Asp Glu Ala Arg Ala Tyr Glu Val Ala 
145                 150                 155                 160 


Thr Asp Lys Lys Ile Val Arg Pro Thr Leu Glu Gly Ser Phe Asp Asp 
                165                 170                 175     


Thr Thr Val Leu Gln Leu Thr Asp Glu Val Ala Lys Ser Ser Tyr Asp 
            180                 185                 190         


Tyr Gly Phe Gln Thr Gly Ala Ile Leu Ala Glu Lys Val Asn Gly Val 
        195                 200                 205             


Tyr Gln Pro Val Ala Ala Gly Phe Asn Lys Val Val Pro Tyr Gln Thr 
    210                 215                 220                 


Tyr Ala Leu Leu Asn Gly Ala Ser Arg Glu Thr Asn Phe Ser Pro Ala 
225                 230                 235                 240 


Asn Asp Met Asn His Tyr Asp Thr Ile His Ala Glu Met Gln Ile Leu 
                245                 250                 255     


Val Glu Ala Ala Lys Gln Gly Ile Ser Leu Lys Asp Lys Thr Leu Phe 
            260                 265                 270         


Val Asn Leu Met Pro Cys Pro Ser Cys Ala Arg Thr Leu Ser Gln Thr 
        275                 280                 285             


Glu Leu Ser Glu Ile Val Tyr Arg Ile Asp His Ser Gly Gly Tyr Ala 
    290                 295                 300                 


Val Asp Leu Leu Thr Lys Val Gly Lys Asp Ile Arg Arg Ile Val Tyr 
305                 310                 315                 320 


<210>  142
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.32; 
       tr|A0A2G6N4N7|A0A2G6N4N7_9DELT polypeptide sequence

<400>  142

Met Lys Glu Arg Thr Val Ser Tyr Ser Asp Arg His Phe Met Ala Glu 
1               5                   10                  15      


Ala Leu Glu Met Ala Glu Ser Ala Leu Thr Gln Gly Glu Phe Pro Val 
            20                  25                  30          


Gly Cys Val Ile Ala Asp Gly Thr Ala Val Val Ala Arg Gly His Arg 
        35                  40                  45              


Thr Gly Thr Thr Ala Gly Ala Val Asn Glu Ile Asp His Ala Glu Ile 
    50                  55                  60                  


Asn Ala Leu Arg His Leu Gly Leu Ala Gly Glu His Leu Asp Arg Thr 
65                  70                  75                  80  


Asp Leu Thr Ile Tyr Ser Thr Met Glu Pro Cys Leu Met Cys Phe Ala 
                85                  90                  95      


Ala Ile Val Leu Ser Gly Ile Asn Arg Ile Val Tyr Ala Tyr Glu Asp 
            100                 105                 110         


Val Met Gly Gly Gly Thr Gly Cys Asp Leu Thr Gly Leu Pro Pro Leu 
        115                 120                 125             


Tyr Arg Asp Ala Pro Leu Thr Leu Val Ala Gly Val Arg Arg Arg Ala 
    130                 135                 140                 


Ser Leu Asn Leu Phe Arg Arg Phe Phe Thr Asp Pro Glu Asn Gly Tyr 
145                 150                 155                 160 


Trp Ala Gly Ser Leu Leu Ser Arg Tyr Thr Leu Asn Gln Thr Lys Asp 
                165                 170                 175     


Ser His Arg Leu 
            180 


<210>  143
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.33; 
       tr|A0A0G0RBB8|A0A0G0RBB8_9BACT polypeptide sequence

<400>  143

Met Gln Ser Val Gln Tyr Asn Lys Leu Thr His Leu Gln Arg Arg Ala 
1               5                   10                  15      


Leu Asp Glu Ala Glu Gln Val Leu Glu Asn Ser Tyr Asn Pro Tyr Ser 
            20                  25                  30          


His Phe Tyr Val Gly Ala Cys Leu Ile Ser Glu Asp Glu Gln Leu Ile 
        35                  40                  45              


Ala Gly Thr Asn Phe Glu Asn Ala Ala Tyr Gly Ser Ala Ile Cys Ala 
    50                  55                  60                  


Glu Arg Ala Ala Val Leu Arg Ala Asn Ala Met Ser Ile Arg Arg Phe 
65                  70                  75                  80  


Arg Gly Ile Ala Ile Ile Ala Arg Gly Glu Asp Phe Asn Thr Thr Glu 
                85                  90                  95      


Val Thr Gly Pro Cys Gly Ser Cys Arg Gln Val Leu Tyr Glu Ile Ser 
            100                 105                 110         


Gln Val Ser Gly Cys Asp Leu Gln Val Ile Leu Ala Thr Ser Lys Lys 
        115                 120                 125             


Asp Lys Ile Val Ile Thr Thr Ile Arg Glu Leu Leu Pro Leu Ala Phe 
    130                 135                 140                 


Gly Pro Leu Asp Leu Gly Val Asp Ile Gly Lys Tyr 
145                 150                 155     


<210>  144
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.34; 
       tr|A0A327L2Q5|A0A327L2Q5_9RHIZ polypeptide sequence

<400>  144

Met Val Thr Ser Arg Asp Gly Glu Asp Glu Ala Met Met Ala Arg Cys 
1               5                   10                  15      


Val Ala Leu Ser Arg Ile Ala Val Gly Lys Gly Glu Tyr Pro Phe Gly 
            20                  25                  30          


Ala Val Val Ala Arg Glu Gly Arg Ile Val Ala Glu Ala Ile Asn Arg 
        35                  40                  45              


Thr Ile Arg Asp Gly Asp Val Ser Arg His Ala Glu Val Ile Ala Leu 
    50                  55                  60                  


Ala Arg Ala Gln Lys Ala Ile Gly Arg Arg Glu Leu Arg Glu Cys Ser 
65                  70                  75                  80  


Leu Tyr Ser Asn Val Glu Pro Cys Ala Met Cys Ser Tyr Cys Ile Arg 
                85                  90                  95      


Glu Ala Trp Val Gly Arg Val Val Tyr Ala Leu Gly Ser Pro Val Met 
            100                 105                 110         


Gly Gly Val Ser Lys Trp Asn Ile Leu Arg Asp Asp Gly Leu Ser Gly 
        115                 120                 125             


Arg Met Pro Gln Val Phe Asp Ala Ala Pro Glu Val Val Ser Gly Val 
    130                 135                 140                 


Leu Val Glu Gln Ala Gln Ala Ala Trp Arg Asp Trp Ser Pro Leu Ala 
145                 150                 155                 160 


Trp Glu Met Ile Thr Leu Arg Gly Leu Met Thr Asp Pro Ser Ala Arg 
                165                 170                 175     


Pro Glu Cys Arg Thr Arg Ala Ala Arg Pro Arg Ser Leu Trp His His 
            180                 185                 190         


Leu Val Ala Leu Ile Glu Arg Pro Pro Arg Pro Tyr Val Asp Pro Thr 
        195                 200                 205             


Ser Ala Ala Glu Gly His Ala Asp Leu 
    210                 215         


<210>  145
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.35; 
       tr|S2DR30|S2DR30_9BACT polypeptide sequence

<400>  145

Met Lys Met Lys Lys Lys Ile Glu Ile Thr Val Ser Leu Glu Val Ile 
1               5                   10                  15      


Gln Lys Ser Glu Trp Ser Lys Glu Asp Arg Ser Leu Ile Glu Arg Ala 
            20                  25                  30          


Ile His Ala Val Glu His Ala His Ala Pro Tyr Ser Asn Phe Met Val 
        35                  40                  45              


Gly Thr Ala Leu Leu Leu Asp Asn Gly Gln Ile Phe Ser Ala Asn Asn 
    50                  55                  60                  


Gln Glu Asn Val Ser Phe Pro Val Gly Ile Cys Ala Glu Arg Ala Val 
65                  70                  75                  80  


Leu Ser Tyr Ala Met Gly Asn Phe Pro Asn Asn Arg Pro Val Lys Leu 
                85                  90                  95      


Ala Val Val Ala Lys Arg Arg Ser Asp Ser Thr Trp Ala Thr Val Thr 
            100                 105                 110         


Pro Cys Gly Leu Cys Arg Gln Thr Ile Asn Glu Tyr Glu Val Lys Phe 
        115                 120                 125             


Gly His Pro Ile Glu Ile Leu Met Leu Asn Pro Gly Glu Glu Ile Leu 
    130                 135                 140                 


Lys Ala Ser Gly Ile Asp Gln Leu Leu Pro Phe Arg Phe Asn Asp Leu 
145                 150                 155                 160 


Asn Ser 
        


<210>  146
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.36; 
       tr|A0A369QGF1|A0A369QGF1_9BACT polypeptide sequence

<400>  146

Met Glu Glu His Glu Lys Trp Met His Trp Cys Leu Asn Leu Ala Gln 
1               5                   10                  15      


Gln Ala Leu Gln Gln Gly Asp Phe Pro Val Gly Ala Val Val Val Gln 
            20                  25                  30          


Lys Gly Lys Leu Ile Gly Gln Gly Val Glu Ala Gly Gln Leu Lys Lys 
        35                  40                  45              


Asp Ile Thr Cys His Ala Glu Met Glu Ala Ile Arg Asp Ala Arg Gln 
    50                  55                  60                  


Thr Ile Asn Thr Ala Asp Leu Gln Asn Cys Ile Leu Tyr Ser Thr His 
65                  70                  75                  80  


Glu Pro Cys Ile Met Cys Ser Tyr Val Ile Arg His His Lys Ile Ser 
                85                  90                  95      


Arg Val Val Val Gly Thr Thr Val Pro Glu Val Gly Gly Ser Ser Ser 
            100                 105                 110         


Ala Tyr Pro Leu Leu Ser Ala Pro Asp Ile Ser Ile Trp Val Ala Pro 
        115                 120                 125             


Pro His Leu Val Thr Gly Val Leu Ala Glu Ala Cys Gln Ala Leu Ser 
    130                 135                 140                 


Gln Ala Tyr Lys Gln Lys Phe Lys Lys 
145                 150             


<210>  147
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.37; 
       tr|A0A1W6X4U4|A0A1W6X4U4_9RHIZ polypeptide sequence

<400>  147

Met Thr Asn Pro Ser Arg Gln Glu Arg Trp Asp Arg Arg Phe Leu Glu 
1               5                   10                  15      


Leu Ala Lys Val Phe Gly Thr Trp Ser Lys Asp Arg Ser Ala Gly Thr 
            20                  25                  30          


Gly Cys Val Ile Val Gly Pro Asp Arg Leu Leu Arg Ala Ser Gly Tyr 
        35                  40                  45              


Asn Gly Phe Ala Arg Gly Ile Asp Asp Glu Val Pro Glu Arg His Glu 
    50                  55                  60                  


Arg Pro Ala Lys Tyr Ser Trp Thr Glu His Ala Glu Arg Asn Ala Ile 
65                  70                  75                  80  


Tyr Asn Ala Ala Lys Leu Gly Ile Ser Leu Asp Gly Cys Thr Ala Tyr 
                85                  90                  95      


Val Asn Trp Phe Pro Cys Ile Asp Cys Ala Arg Ala Ile Val Gln Ala 
            100                 105                 110         


Gly Ile Val Arg Leu Val Gly Leu His Pro Asp His Ala Asp Gln Arg 
        115                 120                 125             


Trp Gly Ser Glu Phe Lys Phe Ala Thr Glu Met Leu Arg Glu Ser Gly 
    130                 135                 140                 


Ile Glu Ile Ile Leu Tyr Asp Ile Pro Glu Leu Ala Ala Arg Lys 
145                 150                 155                 


<210>  148
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.38; 
       tr|A0A238BW09|A0A238BW09_9BILA polypeptide sequence

<400>  148

Met Glu Glu Met Ala Arg Lys Ile Arg Thr Lys Ala Lys Lys Ala Asn 
1               5                   10                  15      


Ser Tyr Cys Asn Thr Met Thr Phe Leu Ile Ser Lys Ala Ser Ile Val 
            20                  25                  30          


Leu Leu Lys Ala Glu Cys Lys Arg Ile Glu Leu Thr Val Val Ile Phe 
        35                  40                  45              


Arg Phe Leu Ile Lys Met Asn Ala Ser Glu Pro Asn Asn Glu Leu Cys 
    50                  55                  60                  


Asp Met Thr Val Ile Lys Ser Met Leu Lys Ile Thr His Val Ile Phe 
65                  70                  75                  80  


Asp Leu Asp Gly Leu Leu Ile Asp Thr Glu Val Val Phe Ser Lys Val 
                85                  90                  95      


Asn Gln Cys Leu Leu Ser Lys Tyr Asn Lys Lys Phe Thr Pro His Leu 
            100                 105                 110         


Arg Gly Leu Val Thr Gly Met Pro Lys Lys Ala Ala Val Thr Tyr Ile 
        115                 120                 125             


Leu Glu His Glu Lys Leu Ser Ala Lys Val Asp Val Asp Glu Tyr Cys 
    130                 135                 140                 


Lys Lys Tyr Asp Glu Met Ala Glu Glu Met Leu Pro Lys Cys Ser Leu 
145                 150                 155                 160 


Met Pro Gly Val Met Lys Leu Val Arg His Leu Lys Thr His Ser Ile 
                165                 170                 175     


Pro Met Ala Ile Cys Thr Gly Ala Thr Lys Lys Glu Phe Glu Ile Lys 
            180                 185                 190         


Thr Arg Tyr His Lys Glu Leu Leu Asp Leu Ile Ser Leu Arg Val Leu 
        195                 200                 205             


Ser Gly Asp Asp Pro Ala Val Lys Arg Gly Lys Pro Ala Pro Asp Pro 
    210                 215                 220                 


Phe Leu Val Thr Met Asp Arg Phe Lys Gln Lys Pro Glu Lys Ala Glu 
225                 230                 235                 240 


Asn Val Leu Val Phe Glu Asp Ala Ala Asn Gly Val Cys Ala Ala Ile 
                245                 250                 255     


Ala Ala Gly Met Asn Val Ile Met Val Pro Asp Leu Thr Tyr Met Lys 
            260                 265                 270         


Ile Pro Glu Gly Leu Gln Asn Lys Ile Asn Ser Phe Ser Asp Asn Leu 
        275                 280                 285             


Ile Ile Ser Asn Asp Leu Asn Val Ala Leu Met Ser Leu Lys Lys Glu 
    290                 295                 300                 


Leu Ser Glu Glu Glu Val His Phe Leu Asn Arg Ala Phe Glu Ile Ala 
305                 310                 315                 320 


Val Asp Ala Val Leu Asn Asn Glu Val Pro Val Gly Cys Val Phe Val 
                325                 330                 335     


Phe Glu Gly Gln Glu Val Ala Phe Gly Arg Asn Asp Val Asn Arg Thr 
            340                 345                 350         


Lys Asn Pro Thr Tyr His Ala Glu Met Val Ala Leu Lys Met Met Lys 
        355                 360                 365             


Gln Trp Cys Met Asp Asn Gly Arg Asp Leu Glu Glu Ile Met Arg Arg 
    370                 375                 380                 


Thr Thr Leu Tyr Val Thr Leu Glu Pro Cys Ile Met Cys Ala Ser Ala 
385                 390                 395                 400 


Leu Tyr His Leu Arg Leu Lys Lys Ile Leu Tyr Gly Ala Ala Asn Glu 
                405                 410                 415     


Arg Phe Gly Gly Leu Val Ser Val Gly Thr Arg Glu Lys Tyr Gly Ala 
            420                 425                 430         


Lys His Phe Ile Glu Ile Met Pro Asn Leu Ser Val Asp Arg Ala Val 
        435                 440                 445             


Lys Leu Leu Lys Glu Phe Tyr Glu Lys Gln Asn Pro Phe Cys Pro Glu 
    450                 455                 460                 


Glu Lys Arg Lys Val Lys Lys Pro Lys Lys Ser Gly Asn Asn Asn Asp 
465                 470                 475                 480 


Asn Ser Asp Asp Ala Val Ala Leu Asn Val 
                485                 490 


<210>  149
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.39; 
       tr|A0A1J5H6Z0|A0A1J5H6Z0_9BACT polypeptide sequence

<400>  149

Met Ala Tyr Gln Pro Ser Glu Lys Phe Met Gln Met Ala Ile Asp Lys 
1               5                   10                  15      


Thr Arg Glu Gly Val Leu Ser Gly Gln Thr Pro Phe Gly Ala Cys Ile 
            20                  25                  30          


Val Lys Asp Gly Lys Val Val Ala Cys Glu His Asn Thr Val Trp Gln 
        35                  40                  45              


Asp Thr Asp Ile Thr Ser His Gly Glu Val His Thr Ile Arg Ala Ala 
    50                  55                  60                  


Cys Lys Ala Ile Gly Ser Ile Asp Leu Ser Gly Cys Ile Leu Tyr Ser 
65                  70                  75                  80  


Thr Cys Glu Pro Cys Pro Met Cys Phe Ser Ala Ile His Trp Ala Arg 
                85                  90                  95      


Ile Asp Thr Val Val Tyr Gly Ala Phe Ile Ala Asp Ala Gln Asp Ala 
            100                 105                 110         


Gly Phe Asn Glu Leu Thr Ile Ser Asn Glu Lys Met Lys Glu Phe Gly 
        115                 120                 125             


Gly Ser Pro Val Asn Phe Ile Ser Gly Phe Met Arg Asp Glu Asn Val 
    130                 135                 140                 


Ala Leu Phe Lys Leu Trp Lys Glu Gln Gly Ala Asn Asn Val Tyr 
145                 150                 155                 


<210>  150
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.40; 
       tr|A0A3C2D945|A0A3C2D945_9BACT polypeptide sequence

<400>  150

Met Lys Thr Thr Glu Ile Arg Ile Ile Val His Glu Tyr Gln Asn Ile 
1               5                   10                  15      


Asp Glu Leu Thr Glu Asn Asp Gln Tyr Leu Leu His Glu Ala Arg Arg 
            20                  25                  30          


Ile Thr Glu Phe Ala Tyr Ala Pro Tyr Ser Gly Phe His Val Gly Ala 
        35                  40                  45              


Ala Ile Leu Leu Gly Asn Gly Met Ile Val Lys Gly Asn Asn Gln Glu 
    50                  55                  60                  


Asn Ser Ala Tyr Pro Ser Gly Leu Cys Ala Glu Arg Val Ala Leu Phe 
65                  70                  75                  80  


Tyr Ala Asn Ala Asn Tyr Pro Asp Ser Glu Val Lys Thr Ile Ala Ile 
                85                  90                  95      


Ser Ala Ala Lys Asn Gly Ile Leu Val Asn Asp Pro Ile Lys Pro Cys 
            100                 105                 110         


Gly Gly Cys Arg Gln Thr Leu Ser Glu Ala Glu Val Arg Phe Gly Ser 
        115                 120                 125             


Pro Ile Arg Ile Ile Leu Asp Gly Gln Asp Ser Ile Leu Val Leu His 
    130                 135                 140                 


Gly Val Glu Ser Leu Leu Pro Leu Ser Phe Ser Lys Lys Asp Leu Ala 
145                 150                 155                 160 


Ser Pro Leu Ala Ala Thr Gly Arg 
                165             


<210>  151
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.41; 
       tr|A0A1I7EYS3|A0A1I7EYS3_9BURK polypeptide sequence

<400>  151

Met Lys Phe Lys Leu Asp Pro Ser Arg Pro Pro Asp Glu Asp Asp Tyr 
1               5                   10                  15      


Tyr Leu Gly Val Ala Leu Ala Val Arg Arg Lys Ala Asn Cys Thr Gly 
            20                  25                  30          


Asn Arg Val Ala Ala Val Ile Val Lys Asn Lys Arg Val Ile Ala Thr 
        35                  40                  45              


Gly Tyr Asn Gly Val Pro Glu Asp Met Pro Asn Cys Leu Asp Gly Gly 
    50                  55                  60                  


Cys Leu Arg Cys Ser Asn Pro Gly Gly Gln Phe Lys Ser Gly Thr Arg 
65                  70                  75                  80  


Tyr Asp Leu Cys Ile Cys Val His Ala Glu Gln Asn Ala Leu Leu Thr 
                85                  90                  95      


Ala Ala Arg Phe Gly Ile Ser Val Glu Gly Ala His Leu Tyr Thr Thr 
            100                 105                 110         


Met Gln Pro Cys Phe Gly Cys Ala Lys Glu Ile Leu Gln Ala Lys Ile 
        115                 120                 125             


Glu Lys Val Phe Tyr Leu His Pro Trp Val Pro Thr Asp Val Asp Pro 
    130                 135                 140                 


Val Met Asp Ala Ala Met Lys Ala Glu Tyr Ala Lys Ile Ile Gly Lys 
145                 150                 155                 160 


Leu Lys Val Lys Lys Leu Asp Phe Asp Asp Pro Val Ala Thr Trp Ala 
                165                 170                 175     


Val Thr Thr Met Arg Gln Ala Ala Leu Ala Ser Asp Lys Asn Pro Asp 
            180                 185                 190         


Lys Lys Thr Pro Pro Lys Thr Ala Lys Lys Lys Val Ala Lys Lys Lys 
        195                 200                 205             


Ser Arg Thr Ser Pro Arg 
    210                 


<210>  152
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.42; 
       tr|H8GQX8|H8GQX8_METAL polypeptide sequence

<400>  152

Met Asn His Glu His Phe Met Arg Arg Ala Ile Glu Leu Ala Arg Gln 
1               5                   10                  15      


Ala Pro Gln Tyr Pro Phe Gly Ala Val Ile Val Arg Arg Asp Asp Gly 
            20                  25                  30          


Gln Cys Val Gly Gln Gly Phe Asn Arg Ser Asp Leu Asn Pro Thr Tyr 
        35                  40                  45              


His Gly Glu Met Val Ala Ile Asn Asp Cys Ala Val Arg His Cys Ala 
    50                  55                  60                  


Glu Asp Trp Arg Gly Phe Asp Leu Tyr Thr Thr Ala Glu Pro Cys Ala 
65                  70                  75                  80  


Met Cys Gln Gly Ala Ile Glu Trp Ala Gly Ile Gly Arg Val Phe Tyr 
                85                  90                  95      


Gly Thr Ser Ile Pro Tyr Leu Gln Lys Leu Gly Trp Trp Gln Ile Asp 
            100                 105                 110         


Leu Arg Ala Ala Glu Val Ser Ala Arg Ala Val Phe Arg Asp Thr Leu 
        115                 120                 125             


Ile Val Gly Gly Ile Leu Glu Thr Glu Cys Asn Ala Leu Phe Ala Ala 
    130                 135                 140                 


Ala Arg Arg Gly Cys Phe Gly Thr Gly Ser Glu 
145                 150                 155 


<210>  153
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.43; 
       tr|A0A0S8HZN3|A0A0S8HZN3_9CHLR polypeptide sequence

<400>  153

Met Asp Glu His Asp Ile Arg Phe Leu Arg Ala Ser Phe Asp Val Ala 
1               5                   10                  15      


Arg Asn Ala Arg Lys Asn Gly Asn His Pro Phe Gly Ala Leu Leu Val 
            20                  25                  30          


Asp Glu His Gly Arg Ile Val Met Glu Ala Glu Asn Thr Val Ile Thr 
        35                  40                  45              


Ala Lys Asp Cys Thr Gly His Ala Glu Thr Asn Leu Met Arg Glu Ala 
    50                  55                  60                  


Ser Ser Lys Tyr Asp Ser Asp Phe Leu Ala Asn Cys Thr Ile Tyr Thr 
65                  70                  75                  80  


Ser Thr Glu Pro Cys Pro Met Cys Ala Gly Ala Ile Phe Trp Ser Asn 
                85                  90                  95      


Val Arg Arg Val Val Tyr Gly Leu Ser Glu Glu Ser Leu Tyr Glu Ile 
            100                 105                 110         


Ala Gly Arg Gly Ser Glu Glu Val Leu Phe Leu Ser Cys Arg Glu Ile 
        115                 120                 125             


Phe Glu Arg Gly Lys Lys Leu Ile Glu Val Ile Gly Pro Leu Leu Glu 
    130                 135                 140                 


Asp Glu Ala Arg Glu Val His Met Gly Phe Trp Arg 
145                 150                 155     


<210>  154
<211>  193
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.44; 
       tr|E3SF31|E3SF31_9CAUD polypeptide sequence

<400>  154

Met Lys Pro Thr Thr Val Leu Gln Ile Ala Tyr Leu Val Ser Gln Glu 
1               5                   10                  15      


Ser Lys Cys Cys Ser Trp Lys Val Gly Ala Val Ile Glu Lys Asn Gly 
            20                  25                  30          


Arg Ile Ile Ser Thr Gly Tyr Asn Gly Ser Pro Ala Gly Gly Val Asn 
        35                  40                  45              


Cys Cys Glu His Ala Glu Glu Gln Gly Trp Leu Leu Asn Lys Pro Lys 
    50                  55                  60                  


Pro Val Leu Ile Pro Gly His Lys Ser Glu Cys Val Arg Phe Ser Gln 
65                  70                  75                  80  


Val Asp Arg Phe Val Leu Ala Lys Ala His Arg Glu Ala His Ser Ala 
                85                  90                  95      


Trp Ser Lys Asn Asn Glu Ile His Ala Glu Leu Asn Ala Ile Leu Phe 
            100                 105                 110         


Ala Ala Arg Met Gly Ser Ser Ile Glu Gly Ala Thr Met Tyr Val Thr 
        115                 120                 125             


Leu Ser Pro Cys Pro Asp Cys Ala Lys Ala Ile Ser Gln Ser Gly Ile 
    130                 135                 140                 


Lys Lys Leu Val Tyr Cys Glu Thr Tyr Asp Lys Asn Ile Pro Gly Trp 
145                 150                 155                 160 


Asp Asp Ile Leu Lys Asn Ala Gly Ile Glu Val Phe Asn Val Pro Lys 
                165                 170                 175     


Arg Ser Leu Asp Lys Leu Asn Trp Glu Asn Ile Asn Glu Phe Cys Gly 
            180                 185                 190         


Glu 
    


<210>  155
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.45; 
       tr|F8AAC6|F8AAC6_THEID polypeptide sequence

<400>  155

Met Ile Arg Ala Pro Trp His Glu Tyr Phe Met Leu Leu Ala Lys Ile 
1               5                   10                  15      


Val Ala Leu Arg Ser Gly Cys Asn Ser Arg Pro Ser Gly Ala Val Ile 
            20                  25                  30          


Val Lys Asn Lys Arg Ile Leu Ala Thr Gly Tyr Asn Gly Pro Met Pro 
        35                  40                  45              


Gly Ala Trp His Cys Thr Asp Arg Gly Pro Gly Tyr Cys Phe Arg Arg 
    50                  55                  60                  


Glu Lys Gly Ile Pro Asp Ile Asp Lys Tyr Asn Phe Cys Arg Ala Thr 
65                  70                  75                  80  


His Ala Glu Ala Asn Ala Ile Ala Gln Ala Ala Arg Phe Gly Ile Ser 
                85                  90                  95      


Val Glu Gly Ala Ser Leu Tyr Cys Thr Leu Ala Pro Cys Tyr Val Cys 
            100                 105                 110         


Leu Lys Leu Ile Ala Ser Ala Gly Ile Lys Lys Val Tyr Tyr Glu His 
        115                 120                 125             


Asp Tyr Gly Ser Arg Asp Phe Glu Arg Asp Gln Phe Trp Lys Glu Ala 
    130                 135                 140                 


Ile Lys Glu Ala Gly Leu Glu Lys Phe Glu Gln Ile Thr Val Ser Gln 
145                 150                 155                 160 


Glu Val Met Glu Gln Leu Gln Glu Ile Leu Pro Tyr Pro Thr Ser Lys 
                165                 170                 175     


Arg Arg Leu Ala Pro Thr Glu Phe Leu Asp Glu Phe Glu Asp Gly Lys 
            180                 185                 190         


Lys Tyr Gly Val Pro Ser Ile Glu Val Leu Phe Asn Lys Leu Asn Tyr 
        195                 200                 205             


Leu Thr Arg Gln Ala Leu Lys Asp Ile Thr Phe Val Ile Glu Lys Thr 
    210                 215                 220                 


Thr Val Thr Glu Glu Pro Glu Gly Ile Ser Phe Tyr Leu Ser Gly Lys 
225                 230                 235                 240 


Met Val Glu Leu Ser Glu Leu Ile Asn Thr Val Lys Lys Gln Ile Asn 
                245                 250                 255     


Ala Asp Gln Asn Phe Tyr Phe Leu Ala Lys His Asn Ala Ile Glu Ala 
            260                 265                 270         


Lys Ile Glu Ile Leu Arg Glu Ala Glu Asn Ile Arg Leu Lys Ala Phe 
        275                 280                 285             


Leu Asn Glu Cys Pro Leu Glu Ser Phe Lys Arg Ile Ala Glu Ser Leu 
    290                 295                 300                 


Asp Tyr Ile Leu Tyr Gln Val Ser Asn Ser Leu Ser Leu Pro Thr Arg 
305                 310                 315                 320 


Leu Glu Leu Ser Val Asn Leu Leu Arg Ile 
                325                 330 


<210>  156
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.46; 
       tr|A0A2H4ZNK4|A0A2H4ZNK4_9EUKA polypeptide sequence

<400>  156

Met Lys Lys Gln Leu Ser Arg Lys Ile Gln Glu Glu Trp Met Ser Arg 
1               5                   10                  15      


Leu Leu Arg Asn Ala Tyr Asp Ala Gly Thr Tyr Gly Glu Val Pro Ile 
            20                  25                  30          


Ala Ala Val Ile Leu Asn Glu Ser Gly Gln Cys Ile Gly Trp Gly Arg 
        35                  40                  45              


Asn Cys Arg Glu Lys Asp Gln Asn Pro Leu Gly His Ala Glu Ile Ile 
    50                  55                  60                  


Ala Leu Arg Gln Ala Ser Tyr Leu Lys Lys Ser Trp Arg Phe Asn Glu 
65                  70                  75                  80  


Cys Thr Met Leu Val Thr Leu Glu Pro Cys Pro Met Cys Ala Gly Ala 
                85                  90                  95      


Leu Leu Gln Ala Arg Ile Asn His Ile Ile Tyr Gly Ala Ser Asp Tyr 
            100                 105                 110         


Lys Arg Gly Gly Phe Gly Gly Val Leu Asp Leu Ser Lys Asn Ser Ser 
        115                 120                 125             


Ala His His Lys Ile Glu Ile Thr Arg Gly Val Lys Ser Ile Gln Ser 
    130                 135                 140                 


Cys Gln Leu Leu Glu Thr Trp Phe Arg Arg Arg Arg Arg Val 
145                 150                 155             


<210>  157
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.47; 
       tr|A0A239N5N1|A0A239N5N1_9PSED polypeptide sequence

<400>  157

Met Glu Gly Arg Ala Gly Ile Ile Pro Phe Asp Glu Gly Gly Ala Ala 
1               5                   10                  15      


Met Gly Pro Ala Glu Glu Asp Ser Pro Met Gln His Leu Ala Tyr Met 
            20                  25                  30          


Arg Glu Ala Leu Ala Leu Ala Arg Ala Asn Val Glu Ala Gly Gly Arg 
        35                  40                  45              


Pro Phe Gly Ala Val Leu Val Arg Asp Gly Glu Val Ile Ala Arg Ala 
    50                  55                  60                  


Ala Asn Gly Thr His Leu Asp His Asp Pro Thr Ala His Ala Glu Leu 
65                  70                  75                  80  


Leu Ala Leu Arg Ala Ala Gly Arg Ala Leu Gly Ser Pro Arg Leu Asp 
                85                  90                  95      


Gly Cys Val Val Tyr Ala Ser Gly His Pro Cys Pro Met Cys Leu Ala 
            100                 105                 110         


Ala Met His Leu Ser Gly Val Ser Ala Ala Tyr Tyr Ala Tyr Ser Asn 
        115                 120                 125             


Ala Asp Gly Glu Pro Tyr Gly Leu Ser Thr Ala Ala Val Tyr Ala Gln 
    130                 135                 140                 


Met Ala Gln Pro Val Glu Trp Gln Ser Leu Pro Leu Gln Ala Leu Arg 
145                 150                 155                 160 


Pro Glu Asp Glu Glu Gly Leu Tyr Gly Phe Trp Arg Glu Arg Arg Pro 
                165                 170                 175     


<210>  158
<211>  199
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.48; 
       tr|A0A328VTR2|A0A328VTR2_9PSED polypeptide sequence

<400>  158

Met His Pro Glu His Leu Ala Leu Leu Gln Gln Ala Pro Ala Ser Thr 
1               5                   10                  15      


His Ala Asp Asp Thr Trp Ala Arg Leu Cys Cys Glu Gln Ala Leu Leu 
            20                  25                  30          


Ala Val Glu Glu Gly Cys Tyr Ala Val Gly Ala Leu Leu Val Asp Gly 
        35                  40                  45              


Ala Gly Glu Leu Leu Cys Ser Gly Arg Asn Gln Val Phe Ala Pro Ala 
    50                  55                  60                  


Tyr Ala Ser Ala Ala His Ala Glu Met Arg Val Leu Asp Gln Leu Glu 
65                  70                  75                  80  


Ala Glu His Ala Gln Val Asp Arg Arg Ser Leu Thr Leu Tyr Val Ser 
                85                  90                  95      


Leu Glu Pro Cys Leu Met Cys Tyr Gly Arg Ile Leu Leu Ala Gly Ile 
            100                 105                 110         


Thr Arg Val Arg Tyr Leu Ala Arg Asp Arg Asp Gly Gly Phe Ala Leu 
        115                 120                 125             


Arg His Gly Arg Leu Pro Pro Ala Trp Ala Asn Leu Ala Ser Gly Leu 
    130                 135                 140                 


Ser Val Val Gln Ala Lys Ala Asp Pro Tyr Trp Leu Asp Leu Ala Glu 
145                 150                 155                 160 


His Ala Ile Gly Arg Leu Gln Asp Arg Gln Thr Leu Arg Gln Arg Val 
                165                 170                 175     


Ile Arg Ala Trp Arg Gly Gln Arg Thr Leu Thr Asp Glu Phe Ser Ser 
            180                 185                 190         


Thr Lys Arg Thr His Ser Gly 
        195                 


<210>  159
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.49; 
       tr|A0A103YG48|A0A103YG48_CYNCS polypeptide sequence

<400>  159

Tyr Ile Arg Glu Leu His Ala Ser Ser Leu Arg Arg Asp Glu His Glu 
1               5                   10                  15      


Ile Gln Asn Pro Lys Ile Leu Val Ile Val Asp Arg Leu Ser Ser Pro 
            20                  25                  30          


Ser Leu His Val Ser Leu Ser Leu Ser Leu Ser Leu Val Ile Phe Pro 
        35                  40                  45              


Pro Phe Ile Pro Leu Asn Gln Thr Pro Thr His Met Glu Asn Ala Lys 
    50                  55                  60                  


Val Val Glu Ala Lys Asp Gly Thr Ile Ala Val Ala Ser Ala Phe Ser 
65                  70                  75                  80  


Gly His Gln Glu Val Val Gln Asp Arg Asp His Lys Phe Leu Thr Arg 
                85                  90                  95      


Ala Val Glu Glu Ala Tyr Lys Gly Val Glu Cys Gly Asp Gly Gly Pro 
            100                 105                 110         


Phe Gly Ala Val Val Val His Lys Asp Glu Val Val Ala Ser Cys His 
        115                 120                 125             


Asn Met Val Leu Lys His Thr Asp Pro Thr Ala His Ala Glu Val Thr 
    130                 135                 140                 


Ala Ile Arg Glu Ala Cys Lys Lys Leu Asn Lys Ile Glu Leu Ser Asp 
145                 150                 155                 160 


Cys Glu Ile Tyr Ala Ser Cys Glu Pro Cys Pro Met Cys Phe Gly Ala 
                165                 170                 175     


Ile His Leu Ser Arg Ile Lys Arg Leu Ile Tyr Gly Ala Lys Ala Glu 
            180                 185                 190         


Ala Ala Ile Ala Ile Gly Phe Asp Asp Phe Ile Ala Asp Ala Leu Arg 
        195                 200                 205             


Gly Thr Gly Phe Tyr Gln Lys Ala His Leu Glu Ile Lys Gln Ala Asp 
    210                 215                 220                 


Gly Asn Gly Ala Met Ile Ala Glu Gln Val Phe Glu Lys Thr Lys Ala 
225                 230                 235                 240 


Lys Phe Ala Ile Asp His Lys Phe Leu Thr Arg Ala Val Glu Glu Ala 
                245                 250                 255     


Tyr Lys Gly Val Glu Cys Gly Asp Gly Arg Pro Phe Gly Ala Leu Val 
            260                 265                 270         


Val His Lys Asp Glu Val Val Val Ser Cys His Asn Met Val Leu Asn 
        275                 280                 285             


Tyr Thr Asp Pro Thr Ala His Ala Glu Ile Thr Ala Ile Arg Glu Ala 
    290                 295                 300                 


Cys Lys Lys Leu Asn Arg Ile Glu Leu Ser Asp Cys Glu Met Tyr Ser 
305                 310                 315                 320 


Ser Cys Glu Pro Cys Pro Met Cys Phe Gly Ala Ile Gln Ile Ser Arg 
                325                 330                 335     


Ile Lys Arg Leu Val Tyr Gly Ala Lys Ala Glu Ala Ser Ile Ala Ser 
            340                 345                 350         


Gly Ile Pro Ile Gly Asp Phe Ile Ser Asp Ala Leu Lys Gly Thr Gly 
        355                 360                 365             


Phe His Glu Lys Ala Asn Phe Glu Ile Lys Gln Ala Asp Gly Asn Gly 
    370                 375                 380                 


Ala Met Ile Ala Glu Gln Val Phe Glu Arg Thr Lys Ala Met Phe Pro 
385                 390                 395                 400 


Lys Arg 
        


<210>  160
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.50; 
       tr|W5M1M8|W5M1M8_LEPOC polypeptide sequence

<400>  160

Asn Ser Ser Thr Arg Glu Ser Arg Val Met Ala Gln Met Glu Ile Asn 
1               5                   10                  15      


Gly Gly Ala Ser Pro Pro Lys Lys Pro Gly Lys Gly Gln Ser Ala Ala 
            20                  25                  30          


Asp Gln Asp Met Ile Thr Gly Leu Ile Asn Lys Ala Leu Gln Ala Lys 
        35                  40                  45              


Glu Phe Ala Tyr Cys Pro Tyr Ser Asn Phe Arg Val Gly Ala Ala Leu 
    50                  55                  60                  


Met Thr Asn Asp Gly Arg Val Phe Thr Gly Cys Asn Val Glu Asn Ala 
65                  70                  75                  80  


Cys Tyr Asn Leu Gly Val Cys Ala Glu Arg Thr Ala Ile Leu Lys Ala 
                85                  90                  95      


Val Ser Glu Gly Tyr Glu Ser Phe Arg Ala Ile Ala Val Ser Ser Asp 
            100                 105                 110         


Leu Gln Asp Gln Phe Ile Ser Pro Cys Gly Ala Cys Arg Gln Val Met 
        115                 120                 125             


Arg Glu Phe Gly Thr Gly Trp Asp Val Phe Leu Thr Lys Val Asp Gly 
    130                 135                 140                 


Ser Tyr Val Arg Met Thr Val Asp Glu Leu Leu Pro Met Ser Phe Gly 
145                 150                 155                 160 


Pro Asp Asp Leu Lys Lys Lys Lys Val Phe Ser Leu Gln Asn Gly His 
                165                 170                 175     


Glu Val Ser Thr Gln Phe Tyr Thr His Ser Pro Cys Glu Ala Gly Glu 
            180                 185                 190         


Asn Asn Asn 
        195 


<210>  161
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.51; 
       tr|A0A3N5YPZ2|A0A3N5YPZ2_9ALTE polypeptide sequence

<400>  161

Met Ser Asn Ser Glu Thr Glu His Ile Gln Ala Leu Val Asp Ala Ala 
1               5                   10                  15      


Gln Ala Ala Gln Lys Gln Ser Tyr Ser Pro Tyr Ser Ser Phe Gln Val 
            20                  25                  30          


Gly Ala Ala Ile Phe Ala Asp Asp Gly Asn Thr Tyr Ser Gly Cys Asn 
        35                  40                  45              


Ile Glu Asn Val Ala Tyr Pro Leu Gly Gln Cys Ala Glu Ala Thr Ala 
    50                  55                  60                  


Ile Gly Met Met Ile Met Gln Gly Ala Lys Arg Ile Glu Asp Ile Met 
65                  70                  75                  80  


Ile Ala Ser Pro Asn Asp Gln Val Cys Pro Pro Cys Gly Gly Cys Arg 
                85                  90                  95      


Gln Lys Ile Ser Glu Phe Gly Thr Ala Glu Thr Lys Ile His Met Val 
            100                 105                 110         


Thr Arg Ser Gly Glu Val Ser Thr Val Thr Leu Gly Glu Leu Leu Pro 
        115                 120                 125             


Leu Ala Phe Asp Ser Leu 
    130                 


<210>  162
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.52; 
       tr|A0A2A9NC86|A0A2A9NC86_9AGAR polypeptide sequence

<400>  162

Met Thr Asn Ser Thr Leu Ser Asn Glu Asp Arg Thr Arg Leu Ile Gln 
1               5                   10                  15      


Gly Ala Phe Gln Ala Arg Lys Lys Thr Tyr Ser Pro Tyr Ser Asn Phe 
            20                  25                  30          


Pro Val Gly Ala Ala Leu Leu Thr Thr Asp Gly Arg Ile Ile Glu Gly 
        35                  40                  45              


Ala Asn Ile Glu Asn Ala Ser Tyr Gly Gly Thr Ile Cys Ala Glu Arg 
    50                  55                  60                  


Thr Ala Ile Val Lys Ala Val Ser Asp Gly Tyr Arg His Phe Ala Gly 
65                  70                  75                  80  


Ile Ala Val Thr Thr Lys Met Pro Thr Arg Val Ser Pro Cys Gly Ile 
                85                  90                  95      


Cys Arg Gln Val Leu Arg Glu Phe Cys Ser Leu Asp Met Pro Val Leu 
            100                 105                 110         


Leu Val Pro Gly Asp Tyr Pro Gln Arg Asn Pro Val Asp Asp Asp Gly 
        115                 120                 125             


Ala Asp Lys Pro Gly Val Ile Thr Glu Gly Gly Val Arg Glu Thr Thr 
    130                 135                 140                 


Leu Gly Ala Leu Leu Pro Asp Ser Phe Gly Pro Glu Asn Leu Pro Pro 
145                 150                 155                 160 


Arg Ala 
        


<210>  163
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.53; 
       tr|A0A2D6RD43|A0A2D6RD43_9GAMM polypeptide sequence

<400>  163

Met Asn Ile Glu Asn Leu Ile Thr Glu Asn Asp Glu Thr Leu Ile Arg 
1               5                   10                  15      


Arg Cys Ile Glu Leu Ala Gly Glu Ser Val Lys Asn Gly Asp Lys Pro 
            20                  25                  30          


Phe Gly Ala Leu Leu Ala Lys Asp Gly Asn Ile Ile Phe Glu Ser Ser 
        35                  40                  45              


Asn Asn Ala Lys Thr Lys Val Pro Tyr His Ala Glu Ile Leu Thr Leu 
    50                  55                  60                  


Met Asp Ala Gln Asp Lys Leu Asn Thr Thr Asp Leu Ser Asp Tyr Ala 
65                  70                  75                  80  


Leu Tyr Ser Asn Cys Glu Pro Cys Pro Met Cys Ser Phe Met Ile Arg 
                85                  90                  95      


Glu Tyr Lys Leu Asp Lys Val Val Phe Ser Val His Ser Pro Tyr Met 
            100                 105                 110         


Gly Gly Gln Ser Arg Trp Asn Ile Leu Glu Asp Asp Val Leu Thr Arg 
        115                 120                 125             


Phe Lys Pro Tyr Phe Ser Lys Pro Pro Asn Val Val Gly Gly Val Leu 
    130                 135                 140                 


Glu Ser Glu Gly Lys Arg Ile Phe Asp Lys Val Gly Leu Trp Met Phe 
145                 150                 155                 160 


Gly Lys Glu 
            


<210>  164
<211>  204
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.54; 
       tr|A0A0H3AVL6|A0A0H3AVL6_BRUO2 polypeptide sequence

<400>  164

Met His Ala Lys Gly Tyr Ser Gln Gln Glu Arg Arg Ile Ile Pro Phe 
1               5                   10                  15      


Ala Asn Arg Phe Arg Phe Arg Glu Leu Cys Ser Asn Lys Ser Leu His 
            20                  25                  30          


Gly Leu Arg Ala Lys Phe Pro Glu Gln Tyr Thr Lys Trp Asp Pro Met 
        35                  40                  45              


Arg Lys Ala Ala Ser Ile Thr Lys Ala Asn Ser Ala Thr Pro Met Asp 
    50                  55                  60                  


Ile Ala Leu Glu Glu Ala His Ala Ala Gly Glu Arg Gly Glu Val Pro 
65                  70                  75                  80  


Ile Gly Ala Val Ile Val Arg Asp Gly Glu Ile Ile Ala Arg Ala Gly 
                85                  90                  95      


Asn Arg Thr Arg Glu Phe Asn Asp Val Thr Ala His Ala Glu Ile Leu 
            100                 105                 110         


Thr Ile Arg Gln Ala Gly Glu Met Leu Gly Ser Glu Arg Leu Ile Asp 
        115                 120                 125             


Cys Asp Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Ala Ala 
    130                 135                 140                 


Ile Ser Phe Ala Arg Ile Arg Arg Leu Tyr Tyr Gly Ala Ser Asp Pro 
145                 150                 155                 160 


Lys Gly Gly Gly Ile Glu His Gly Gly Arg Phe Tyr Thr Gln Pro Thr 
                165                 170                 175     


Cys His His Ala Pro Glu Ile Tyr Pro Gly Phe Cys Glu Ala Asp Ala 
            180                 185                 190         


Arg Lys Ile Leu Lys Asp Phe Phe Arg Glu Lys Arg 
        195                 200                 


<210>  165
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.55; 
       tr|A0A242H531|A0A242H531_9ENTE polypeptide sequence

<400>  165

Met Phe Ile Val Lys Asn Asn Ile Glu Val Ile Gln Gln Gln Ala Glu 
1               5                   10                  15      


Leu Asp Ala Lys Phe Met Lys Gln Ala Leu Lys Leu Ala Lys Asp Ala 
            20                  25                  30          


Ser Asn Asn Gly Asn Glu Pro Phe Gly Ala Val Leu Val Lys Asn Asp 
        35                  40                  45              


Lys Val Ile Leu Thr Gly Glu Asn Gln Ile His Thr Glu Ser Asp Pro 
    50                  55                  60                  


Thr Tyr His Ala Glu Leu Gly Ile Ile Arg Asp Phe Cys Thr Ser Gln 
65                  70                  75                  80  


Lys Ile Thr Asp Leu Ser Glu Tyr Thr Leu Tyr Thr Ser Cys Glu Pro 
                85                  90                  95      


Cys Cys Met Cys Ala Gly Ala Met Val Trp Ser Asn Leu Asp Arg Met 
            100                 105                 110         


Val Tyr Gly Leu Gly His Asp Glu Leu Ala Glu Ile Ala Gly Phe Asn 
        115                 120                 125             


Ile Met Ile Gly Ser Glu Glu Ile Phe Ser Lys Ser Pro Asn Arg Pro 
    130                 135                 140                 


Glu Val Ala Lys Gly Val Leu Lys Glu Ala Ala Val Pro Val Tyr Val 
145                 150                 155                 160 


Asp Tyr Phe Gln Arg 
                165 


<210>  166
<211>  181
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.56; 
       tr|A0A2R6XZE2|A0A2R6XZE2_9BACL polypeptide sequence

<400>  166

Met Ser Gly Arg Ile Ser Trp His Glu Tyr Phe Met Ala Gln Ala Lys 
1               5                   10                  15      


Leu Ile Ala Leu Arg Ala Thr Cys Thr Arg Leu Met Val Gly Ala Val 
            20                  25                  30          


Ile Val Arg Asp Arg Arg Val Ile Ala Gly Gly Tyr Asn Gly Ser Ile 
        35                  40                  45              


Ala Gly Asp Glu His Cys Ile Asp Val Gly Cys Lys Val Arg Asp Gly 
    50                  55                  60                  


His Cys Ile Arg Thr Ile His Ala Glu Gln Asn Ala Leu Met Gln Cys 
65                  70                  75                  80  


Ala Lys Phe Gly Val Ser Thr Asp Gly Ala Glu Leu Tyr Val Thr His 
                85                  90                  95      


Phe Pro Cys Leu Asn Cys Thr Lys Leu Leu Ile Gln Ala Gly Ile Arg 
            100                 105                 110         


His Ile Tyr Tyr Glu Val Pro Tyr Arg Val Asp Pro Tyr Ala Ile Glu 
        115                 120                 125             


Leu Leu Glu Lys Ala Gly Val Gly Thr Thr Gln Ile Thr Val Asp Leu 
    130                 135                 140                 


Asn Ala Tyr Val Gln Val Met Ser Lys Val Ser Thr Asp Pro Ala Leu 
145                 150                 155                 160 


Thr Tyr Val Pro Glu Ser Lys Ala Gln Lys Asp Glu Tyr Gly Gln Ser 
                165                 170                 175     


Val Gly Lys Ile Val 
            180     


<210>  167
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.57; 
       tr|A0A139SHT6|A0A139SHT6_9BACT polypeptide sequence

<400>  167

Met Ser Glu Ala Asn Ala Ser Ser Glu Ser Leu Pro Ser Arg Asn Ser 
1               5                   10                  15      


Pro Val Glu Leu Ile Ala Glu Ala Ala Gly Lys Phe Gly Arg Arg Pro 
            20                  25                  30          


Thr Trp Asp Glu Tyr Phe Met Ala Thr Ala Val Leu Ile Ser Thr Arg 
        35                  40                  45              


Ser Ser Cys Glu Arg Leu Asn Val Gly Cys Val Ile Val Thr Ala Gly 
    50                  55                  60                  


Glu Ser His Lys Asn Arg Ile Val Ala Ala Gly Tyr Asn Gly His Leu 
65                  70                  75                  80  


Pro Gly Ser Pro His Thr Ser Arg Met Arg Asp Gly His Glu Gln Ala 
                85                  90                  95      


Thr Val His Ala Glu Gln Asn Ala Ile Ser Asp Ala Ala Arg Arg Gly 
            100                 105                 110         


Ser Ser Val Glu Gly Cys Thr Ala Tyr Val Thr His Tyr Pro Cys Ile 
        115                 120                 125             


Asn Cys Ala Lys Ile Leu Ala Ser Ala Gly Ile Ala Lys Ile Cys Tyr 
    130                 135                 140                 


Arg Leu Asp Tyr His Asn Asp Pro Leu Val Lys Pro Met Leu Ala Glu 
145                 150                 155                 160 


Ala Gly Ile Glu Ile Val Gln Leu Gly Glu Ala Ala Ser 
                165                 170             


<210>  168
<211>  170
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.58; 
       tr|A0A261DBH2|A0A261DBH2_9RICK polypeptide sequence

<400>  168

Met Val Met Lys Lys Lys Leu Ile Thr Val Lys Arg Ser Thr Glu Phe 
1               5                   10                  15      


Asn Asn Phe Phe Met Glu Glu Ala Leu Lys Gln Ala Gln Phe Ala Leu 
            20                  25                  30          


Asp Lys Asn Glu Ile Pro Val Gly Ala Ile Ile Val Asn Arg Ile Thr 
        35                  40                  45              


Asn Lys Val Ile Ala Lys Ala His Asn Ile Val Glu Gln Thr Lys Asn 
    50                  55                  60                  


Pro Val Leu His Ala Glu Ile Val Ala Ile Asn Gln Ser Cys Gln Ile 
65                  70                  75                  80  


Leu Ser Ser Lys Asn Leu Ser Asp Cys Asp Met Tyr Val Thr Leu Glu 
                85                  90                  95      


Pro Cys Val Met Cys Ser Gly Ala Ile Ser Phe Ala Arg Ile Gly Arg 
            100                 105                 110         


Leu Phe Tyr Ala Ala Asn Asp Pro Lys Gln Gly Ala Ile Glu Asn Gly 
        115                 120                 125             


Gly Arg Phe Phe Asn Ser Lys Ser Cys Phe Tyr Arg Pro Glu Ile Tyr 
    130                 135                 140                 


Ser Gly Phe Ser Ala Lys Ile Ser Glu Asn Leu Ile Lys Glu Phe Phe 
145                 150                 155                 160 


Tyr Asn Val Arg Tyr Gln Lys Cys Asn Pro 
                165                 170 


<210>  169
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.59; 
       tr|A0A2N0XZK6|A0A2N0XZK6_9VIBR polypeptide sequence

<400>  169

Met Thr Asp Asn Ser Leu His Glu Ser Tyr Met Arg Gln Ala Phe Glu 
1               5                   10                  15      


Leu Ser Lys Ser Ala Leu Pro Gly Cys Arg Pro Asn Pro Pro Val Gly 
            20                  25                  30          


Cys Val Phe Val Lys Asp Gly Glu Val Val Ser Ser Gly Phe Ser Gln 
        35                  40                  45              


Pro Pro Gly Asn His His Ala Glu Ala Gly Ala Ile Ala Ala Tyr Thr 
    50                  55                  60                  


Gly Ser Tyr Asp Gly Leu Val Ala Tyr Val Thr Leu Glu Pro Cys Ser 
65                  70                  75                  80  


Phe Gln Gly Arg Thr Pro Ser Cys Ala Lys Ala Leu Val Arg Val Arg 
                85                  90                  95      


Pro Glu Lys Val Tyr Val Ala Ile Leu Asp Pro Asp Thr Arg Asn Ser 
            100                 105                 110         


Gly Ala Gly Ile Lys Ile Leu Glu Asp Ala Gly Ile Asp Val Glu Val 
        115                 120                 125             


Gly Leu Leu Gly Glu Glu Val Ala Ser Phe Leu Asn Pro Tyr Leu Ile 
    130                 135                 140                 


Arg Asn 
145     


<210>  170
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.60; 
       tr|A0A1V5R0F9|A0A1V5R0F9_9BACT polypeptide sequence

<400>  170

Met Thr Lys Lys Glu Thr Thr Lys Leu His Ala Leu Asp Asp Phe Cys 
1               5                   10                  15      


Met Lys Lys Ala Leu Leu Leu Ala Lys Arg Ala Phe Arg Ala Asp Glu 
            20                  25                  30          


Val Pro Val Gly Ala Leu Val Val Asp Ser Ser Asn Lys Val Ile Gly 
        35                  40                  45              


Arg Gly Tyr Asn Gln Val Glu Lys Arg Lys Ser Gln Arg Ala His Ala 
    50                  55                  60                  


Glu Gln Leu Ala Ile Glu Gln Ala Cys Lys Lys Ile Gly Asp Trp Arg 
65                  70                  75                  80  


Leu Glu Gly Cys Thr Leu Tyr Val Thr Leu Glu Pro Cys Thr Met Cys 
                85                  90                  95      


Met Gly Leu Ile Lys Leu Ser Arg Ile Glu Arg Val Val Phe Gly Ala 
            100                 105                 110         


Ala Ser Pro Leu Phe Gly Tyr Gln Leu Asp Lys Asn Arg Lys Ser Gln 
        115                 120                 125             


Leu Tyr Lys Lys Gly Val Ile Lys Ile Arg Lys Gly Val Gly Lys Ala 
    130                 135                 140                 


Thr Ala Ala Ala Leu Leu Lys Asp Phe Phe Lys Asn Lys Arg Met 
145                 150                 155                 


<210>  171
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.61; 
       tr|A0A2W0H8Y3|A0A2W0H8Y3_9BACI polypeptide sequence

<400>  171

Met Lys Asn Asn Gly Arg Leu Asp His Glu Tyr Phe Met Thr Glu Ala 
1               5                   10                  15      


Leu Gln Glu Ala Lys Glu Ala Gly Gln Arg Gly Asp Leu Pro Ile Gly 
            20                  25                  30          


Ala Val Ile Val His Asn Gly Arg Ile Ile Ala Arg Gly Ser Asn Met 
        35                  40                  45              


Arg Lys Thr Ala Gly Ile Lys Ile Ser His Ala Glu Asn Asn Ala Met 
    50                  55                  60                  


His Asn Cys Ala Pro Tyr Leu Met Lys His Ala Ser Glu Cys Val Ile 
65                  70                  75                  80  


Tyr Thr Thr Leu Glu Pro Cys Ile Met Cys Leu Thr Thr Leu Val Met 
                85                  90                  95      


Ala Asn Ile Asp Ser Ile Val Phe Ala Ala Asp Asp Lys Tyr Met Asn 
            100                 105                 110         


Met Lys Pro Phe Ile Asp Ala Asn Ser Tyr Ile Arg Asp Arg Ile His 
        115                 120                 125             


Gln Tyr Lys Gly Gly Val Cys Arg Gly Glu Ser Glu Ala Leu Leu Arg 
    130                 135                 140                 


Lys Tyr Ser Pro Tyr Ala Ala Glu Leu Ala Leu Asn Gly Thr His Pro 
145                 150                 155                 160 


His His Arg Lys Gly Gly Ala 
                165         


<210>  172
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.62; 
       tr|A0A261BDB7|A0A261BDB7_CAERE polypeptide sequence

<400>  172

Leu Tyr Lys Leu Tyr Ile Phe Arg Met Thr Thr Thr Lys Ala Asn Leu 
1               5                   10                  15      


Thr Gln Phe Glu Gln Glu Leu Val Asp Lys Ala Val Gly Ala Met Glu 
            20                  25                  30          


Lys Ala Tyr Cys Lys Tyr Ser Gly Phe Lys Val Gly Ala Ala Leu Val 
        35                  40                  45              


Cys Glu Asp Gly Glu Ile Ile Ile Gly Ala Asn His Glu Asn Ala Ser 
    50                  55                  60                  


Tyr Gly Ala Thr Ile Cys Ala Glu Arg Ser Ala Met Val Thr Ala Leu 
65                  70                  75                  80  


Thr Lys Gly His Arg Lys Phe Lys Leu Leu Ala Val Ala Thr Glu Leu 
                85                  90                  95      


Glu Ala Pro Cys Ser Pro Cys Gly Ile Cys Arg Gln Tyr Leu Ile Glu 
            100                 105                 110         


Phe Gly Asp Tyr Lys Val Ile Leu Gly Ser Ser Thr Ser Asp Gln Ile 
        115                 120                 125             


Ile Glu Thr Thr Thr Tyr Gly Leu Leu Pro Tyr Ala Phe Thr Pro Lys 
    130                 135                 140                 


Ser Leu Asp Asp His Glu Lys Glu Ala Glu Glu Arg Asn His Gln Glu 
145                 150                 155                 160 


Gly Glu Lys Lys His 
                165 


<210>  173
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.63; 
       tr|A0A2E1PHI6|A0A2E1PHI6_9GAMM polypeptide sequence

<400>  173

Met Lys Glu Leu Leu Ile His Ser Trp Leu Met Leu Asn Ser Asn Ser 
1               5                   10                  15      


Lys Leu Ile Met Glu Arg Val Ile Glu Leu Ser Glu Ile Asn Leu Lys 
            20                  25                  30          


Asn Gly Lys Ile Pro Ile Ala Ala Val Ile Val Asp Lys Lys Asn Tyr 
        35                  40                  45              


Glu Ile Ile Ser Glu Ser Gln Asn Glu Asp Ser Pro Ile Gly His Ala 
    50                  55                  60                  


Glu Leu Leu Ala Ile Thr Lys Ala Leu Lys Lys Leu Asn Thr Asn Arg 
65                  70                  75                  80  


Leu Asp Ser Thr Asn Leu Phe Val Thr Ile Glu Pro Cys Pro Met Cys 
                85                  90                  95      


Ala Tyr Ala Ile Ser Lys Cys His Ile Asn Arg Leu Tyr Phe Gly Ser 
            100                 105                 110         


Glu Asp Glu Lys Gly Gly Gly Val Ile Asn Gly Pro Arg Ile Phe Glu 
        115                 120                 125             


Ser His Asn Leu Lys Lys Ile Asp Tyr Val Ser His Cys Tyr His Glu 
    130                 135                 140                 


Lys Thr Thr Gln Leu Met Gln Ser Phe Phe Gln Leu Lys Arg Asn Gln 
145                 150                 155                 160 


Gln Leu 
        


<210>  174
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.64; 
       tr|A0A378LUA7|A0A378LUA7_9GAMM polypeptide sequence

<400>  174

Met Asp Thr Ile Ile Lys Lys Met Ile Ser Asn Ala His Asn Thr Leu 
1               5                   10                  15      


Ala His Ser Tyr Ser Pro Tyr Ser Lys Phe Ser Val Ala Ser Cys Ile 
            20                  25                  30          


Cys Thr Asp Lys Asp Asn Phe Tyr Thr Gly Val Asn Val Glu Asn Ser 
        35                  40                  45              


Ala Tyr Gly Leu Ala Ile Cys Ala Glu Thr Ser Ala Ile Ser Ala Met 
    50                  55                  60                  


Val Thr Ala Gly Glu Lys Arg Ile Lys Ser Met Val Val Met Ala Gly 
65                  70                  75                  80  


Thr Asn Ile Leu Cys Ser Pro Cys Gly Ala Cys Arg Gln Arg Ile Tyr 
                85                  90                  95      


Glu Phe Ser Thr Pro Asp Thr Leu Ile His Leu Cys Asp Lys Asn Ser 
            100                 105                 110         


Ile Leu Arg Thr Phe Lys Ile Asn Glu Leu Leu Pro Glu Ala Phe Lys 
        115                 120                 125             


Phe Asp Phe Asn Pro 
    130             


<210>  175
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.65; 
       tr|A0A139HQ78|A0A139HQ78_9PEZI polypeptide sequence

<400>  175

Met Ala Asp Ser Leu Lys Ser Lys Pro Gly His Ala Arg His Asp Thr 
1               5                   10                  15      


Ala Leu Ile His Gly Leu Ser Gln Ser Asp Val Gln Lys Leu Ser Glu 
            20                  25                  30          


Ser Cys Val Asp Ala Lys Ser Lys Ala Tyr Cys Pro Tyr Ser His Phe 
        35                  40                  45              


Arg Val Gly Cys Ala Val Leu Leu Ala Asn Gly Asp Val Val Gln Gly 
    50                  55                  60                  


Ala Asn Val Glu Asn Ala Ala Tyr Pro Val Gly Thr Cys Ala Glu Arg 
65                  70                  75                  80  


Val Ala Leu Gly Thr Ala Val Gly Ala Lys Lys Gly Asp Phe Arg Ala 
                85                  90                  95      


Leu Ala Val Ser Thr Asp Ile Ser Pro Pro Ala Ser Pro Cys Gly Met 
            100                 105                 110         


Cys Arg Gln Phe Ile Arg Glu Phe Cys Glu Leu Asn Thr Pro Ile Leu 
        115                 120                 125             


Met Tyr Asp Lys Asp Gly Lys Ser Val Val Met Thr Leu Glu Gln Leu 
    130                 135                 140                 


Leu Pro Met Ser Phe Gly Pro Asp Lys Leu Leu Pro Pro Gly Gln Leu 
145                 150                 155                 160 


Glu Asn Gly Leu Met Gln Thr Gln Thr Gln Ser Ser Phe Val Thr Arg 
                165                 170                 175     


Ala Phe Ser Thr Thr Ser Ser Arg Arg Gln Asp Asp Thr Pro Gln Val 
            180                 185                 190         


Pro Gln Ser His Tyr Asp Phe Phe Pro Gln Thr Phe Pro Gln Gly Pro 
        195                 200                 205             


Pro Pro Lys Thr Ser Phe Ser Pro Asp Leu Lys Gln Leu Arg Lys Glu 
    210                 215                 220                 


Phe Leu Gln Leu Gln Ala Lys Ala His Pro Asp Leu Ala Pro Gln Asp 
225                 230                 235                 240 


Gln Lys Arg Arg Ala Glu Ala Leu Ser Met Arg Ile Asn Glu Ala Tyr 
                245                 250                 255     


Lys Thr Leu Gln Ser Pro Leu Arg Arg Ala Gln Tyr Leu Leu Ser Gln 
            260                 265                 270         


Gln Gly Ile Asp Val Glu Asp Glu Thr Ala Lys Leu Asp Asp Ser Ser 
        275                 280                 285             


Leu Leu Met Glu Val Met Glu Ala Arg Glu Ala Val Glu Glu Val Glu 
    290                 295                 300                 


Asp Glu Glu Gln Leu Asn Glu Ile Arg Ala Glu Asn Asn Gly Arg Ile 
305                 310                 315                 320 


Glu Glu Ser Val Arg Val Leu Glu Asp Ala Phe Arg Asp Asn Glu Phe 
                325                 330                 335     


Glu Lys Ala Ala Gln Glu Ala Ile Arg Leu Arg Tyr Trp Val Asn Ile 
            340                 345                 350         


Glu Glu Ser Ile Gln Gly Trp Glu Lys Gly Asn Gly Gly Gly Ile Leu 
        355                 360                 365             


His His 
    370 


<210>  176
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.66; 
       tr|A0A2A9FXV0|A0A2A9FXV0_9VIBR polypeptide sequence

<400>  176

Met Cys Asn Leu Lys Glu Asn Lys Asp Met Asp Lys Tyr Phe His Phe 
1               5                   10                  15      


Ala Cys Asp Ala Thr Ile Glu Gly Met Arg Glu Gly Thr Gly Gly Pro 
            20                  25                  30          


Phe Gly Ala Thr Leu Thr Arg Asn Gly Glu Val Val Cys Ser Val Ala 
        35                  40                  45              


Asn Thr Val Leu Lys Asp Met Asp Ile Ser Gly His Ala Glu Met Val 
    50                  55                  60                  


Ala Val Arg Glu Ala Cys Lys Lys Leu Asp Thr Leu Asp Leu Ser Asp 
65                  70                  75                  80  


Cys Val Met Tyr Ala Thr Cys Glu Pro Cys Pro Met Cys Val Ser Val 
                85                  90                  95      


Met Leu Trp Ala Gly Ile Lys Thr Cys Tyr Tyr Ala Ser Thr His Leu 
            100                 105                 110         


Asp Ala Ala Lys His Gly Phe Ser Asp Gln Gln Leu Arg Asp Tyr Leu 
        115                 120                 125             


Asp Gly Ser Asp Thr Ser Thr Leu Asn Met Val His Ile Glu Asp Asn 
    130                 135                 140                 


Arg Asp Asp Cys Ala Lys Ile Trp Thr Glu Phe Arg His Leu Asn Glu 
145                 150                 155                 160 


Thr Lys Asn Asp Gly 
                165 


<210>  177
<211>  509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.67; 
       tr|A0A1A8AG96|A0A1A8AG96_NOTFU polypeptide sequence

<400>  177

Met Glu His Ser Asp Arg Trp Ser Arg Ala Glu Pro Gly Leu Ser Thr 
1               5                   10                  15      


Ser Ser Arg Glu Thr Arg Asp Gly Ser Thr Gln Thr Asp Cys Lys Leu 
            20                  25                  30          


Gln Gly His Gly Pro Arg Leu Ser Lys Val Asn Leu Phe Thr Leu Leu 
        35                  40                  45              


Ser Leu Trp Met Glu Leu Phe Pro Gln Glu Gln Asp Glu Glu Asn Gly 
    50                  55                  60                  


Gln Ser Gln Ile Arg Arg Ser Gly Leu Val Val Val Arg Glu Gly Lys 
65                  70                  75                  80  


Val Val Gly Leu His Cys Ser Gly Ala Asp Leu His Ala Gly Gln Ala 
                85                  90                  95      


Ala Ile Leu Gln His Gly Ala Ser Leu Ala Asn Cys Gln Leu Phe Phe 
            100                 105                 110         


Ser Arg Arg Pro Cys Ala Thr Cys Leu Lys Met Ile Ile Asn Ala Gly 
        115                 120                 125             


Val Arg Gln Ile Thr Phe Trp Pro Gly Asp Pro Glu Ile Ser Met Leu 
    130                 135                 140                 


Thr Ser Asn Gln Thr His Ser Gln Arg Thr Ser Gln Ser Ile Thr Glu 
145                 150                 155                 160 


Ala Ser Leu Asp Ala Thr Ala Val Glu Lys Leu Lys Ser Asn Ser Arg 
                165                 170                 175     


Pro Gln Ile Cys Val Leu Met Gln Pro Leu Ala Pro Gly Val Leu Gln 
            180                 185                 190         


Phe Val Asp Glu Thr Ser Arg Arg Ser Asp Phe Met Glu Arg Met Met 
        195                 200                 205             


Asp Asp Asp Pro Glu Leu Asp Ser Glu Lys Leu Phe Asn Ser Asp Arg 
    210                 215                 220                 


Leu Arg His Leu Lys Asp Phe Cys Arg His Phe Leu Ile Gln Thr Asp 
225                 230                 235                 240 


Gln Arg His Lys Asp Ile Leu Ser Gln Met Gly Leu Lys Asn Phe Cys 
                245                 250                 255     


Val Glu Pro Tyr Phe Ser Asn Leu Arg Ser Asn Met Thr Glu Leu Val 
            260                 265                 270         


Glu Val Leu Ala Ala Val Ala Ala Gly Met Pro Gln Gln His Tyr Gly 
        275                 280                 285             


Phe Tyr Arg Glu Glu Ser Leu Ser Leu Asp Pro His Pro Val Asp Val 
    290                 295                 300                 


Ser Gln Ala Val Ala Arg His Cys Ile Val Gln Ala Arg Leu Leu Ser 
305                 310                 315                 320 


Tyr Arg Thr Glu Asp Pro Lys Val Gly Val Gly Ala Val Ile Trp Ala 
                325                 330                 335     


Lys Gly Gln Ser Ala Cys Cys Cys Gly Thr Gly Arg Leu Tyr Leu Ile 
            340                 345                 350         


Gly Cys Gly Tyr Asn Ala Tyr Pro Ala Gly Ser Lys Tyr Ala Glu Tyr 
        355                 360                 365             


Pro Gln Met Asp Asn Lys Gln Glu Asp Arg Glu Arg Arg Lys Tyr Arg 
    370                 375                 380                 


Tyr Ile Val His Ala Glu Gln Asn Ala Leu Thr Phe Arg Thr Arg Asp 
385                 390                 395                 400 


Ile Lys Pro Asp Glu Cys Ser Met Leu Phe Val Thr Lys Cys Pro Cys 
                405                 410                 415     


Asp Glu Cys Ile Pro Leu Ile Arg Gly Ala Gly Val Lys His Ile Tyr 
            420                 425                 430         


Thr Ser Asp Gln Asp Arg Asp Lys Asp Lys Gly Asp Ile Ser Tyr Leu 
        435                 440                 445             


Arg Phe Gly Ser Leu Lys Gly Val Cys Lys Phe Ile Trp Gln Arg Ser 
    450                 455                 460                 


Pro Pro Val Ser Ser Ala Ser Ser Leu His Leu Thr Asn Gly Cys Val 
465                 470                 475                 480 


Gly Lys His Val Arg Gln Ala Glu Gln Gln Ile Tyr Lys Asn Lys Lys 
                485                 490                 495     


Leu Cys Thr Lys Gly Ser Ser Gly Ser Ser Asp Ile Cys 
            500                 505                 


<210>  178
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.68; 
       tr|A0A3E2VN88|A0A3E2VN88_9FIRM polypeptide sequence

<400>  178

Met Glu Lys Glu Ile Thr Asn Met Asp Lys Gln Lys Leu Ile Gln Met 
1               5                   10                  15      


Ala Val Asp Gly Leu Gly Arg Ser Tyr Ala Pro Tyr Ser His Phe His 
            20                  25                  30          


Val Ser Ala Ala Leu Leu Cys Ala Asp Gly Thr Val Tyr Thr Gly Asn 
        35                  40                  45              


Asn Ile Glu Asn Ala Ala Tyr Thr Pro Ser Val Cys Ala Glu Arg Cys 
    50                  55                  60                  


Ala Ile Phe Lys Ala Val Gly Asp Gly Arg Arg Glu Phe Glu Ala Ile 
65                  70                  75                  80  


Ala Val Cys Gly Gly Pro Asp Gly Val Ile Glu Asp Tyr Cys Pro Pro 
                85                  90                  95      


Cys Gly Val Cys Arg Gln Val Met Arg Glu Phe Cys Asp Pro Ser Ser 
            100                 105                 110         


Phe Arg Val Leu Val Ala Lys Thr Ala Glu Asp Tyr Arg Glu Tyr Thr 
        115                 120                 125             


Leu Glu Gln Leu Leu Pro Asp Gly Phe Gly Pro Asp His Leu Thr Gly 
    130                 135                 140                 


Ser Gly Glu Arg 
145             


<210>  179
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.69; 
       tr|A0A2D5ZRJ2|A0A2D5ZRJ2_9BACT polypeptide sequence

<400>  179

Met Ala Arg Pro Val His Leu His Thr Gly Glu Arg Arg Thr Glu Glu 
1               5                   10                  15      


Gly Ala Thr Glu Ser Arg Ala Val Ala Ala Val Ala Thr Ala Ile Thr 
            20                  25                  30          


Arg Ala Pro Arg Ala Pro Pro Arg Pro Ala Thr Gly Arg Glu Arg Asp 
        35                  40                  45              


Gly Pro Pro Pro Arg Arg Val Phe Gly Gly Gly Leu Arg Val Gly Asp 
    50                  55                  60                  


Pro Ser Gly Tyr Asp Arg Gly Glu Ser Lys Pro Ile Gly Gly Pro Leu 
65                  70                  75                  80  


Thr Glu Lys Arg Ser Asp Trp His Ser Tyr Phe Met Arg Ile Ala Gly 
                85                  90                  95      


Glu Val Ala Thr Arg Ala Thr Cys Asp Arg Lys His Val Gly Ala Val 
            100                 105                 110         


Ile Val Arg Asn Arg Thr Ile Leu Ser Thr Gly Tyr Asn Gly Ser Ile 
        115                 120                 125             


Arg Gly Met Pro His Cys Asp Asp Val Gly His Asp Met Val Asp Gly 
    130                 135                 140                 


His Cys Ile Ala Thr Ile His Ala Glu Ala Asn Ala Ile Leu Gln Ala 
145                 150                 155                 160 


Ala Arg Asn Gly Val Met Ile Gln Asp Gly Ser Ile Tyr Ile Thr Ala 
                165                 170                 175     


Ser Pro Cys Trp Asn Cys Phe Lys Leu Val Ala Asn Ala Gly Leu Lys 
            180                 185                 190         


Arg Val Tyr Tyr Gly Glu Phe Tyr Arg Asp Lys Arg Ser Phe Glu Val 
        195                 200                 205             


Ala Arg Arg Leu Gly Ile Asp Leu Met His Ile Glu Val 
    210                 215                 220     


<210>  180
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.70; 
       tr|A0A1B8WPS3|A0A1B8WPS3_9BACI polypeptide sequence

<400>  180

Met Glu Gly Val Gln Leu Ile Tyr Gln Phe Gln Trp Gly Asn Leu Ile 
1               5                   10                  15      


Met Thr Val Asn Lys Glu Asp Leu Tyr Leu Ile Asp Val Ala Arg Asn 
            20                  25                  30          


Thr Ile Lys Thr Leu Tyr Val Asp Gly Lys His His Val Gly Ala Ala 
        35                  40                  45              


Val Arg Thr Lys Thr Gly Lys Ile Tyr Ser Ala Val His Leu Glu Ala 
    50                  55                  60                  


Asn Ile Gly Arg Val Ser Val Cys Ala Glu Ala Ile Ala Leu Gly Lys 
65                  70                  75                  80  


Ala Ile Ser Glu Gly Glu Ser Glu Phe Asp Thr Ile Val Ala Val Arg 
                85                  90                  95      


His Pro Asp Pro Thr Gln Glu Asn Gln Lys Ile Glu Val Val Ser Pro 
            100                 105                 110         


Cys Gly Ile Cys Arg Glu Leu Ile Ser Asp Tyr Gly Lys Gly Thr Asn 
        115                 120                 125             


Val Ile Leu Lys Asn Lys Glu Gly Tyr Ile Lys Thr Val Ile Ser Asp 
    130                 135                 140                 


Leu Leu Pro Asn Lys Tyr Ile Arg Glu Asp Asn 
145                 150                 155 


<210>  181
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.71; 
       tr|A0A1W5ZQK9|A0A1W5ZQK9_9BACI polypeptide sequence

<400>  181

Met Asn Arg Phe Met Glu Arg Ala Val Ser Leu Ala Ala Glu Asn Val 
1               5                   10                  15      


Arg Val Gly Gly Gln Pro Phe Gly Ala Val Leu Val Lys Asp Asp Glu 
            20                  25                  30          


Leu Val Ala Glu Gly Val Asn Glu Met His Leu Asn Tyr Asp Val Ser 
        35                  40                  45              


Gly His Ala Glu Leu Leu Ala Ile Arg Arg Ala Gln Gly Glu Leu Gln 
    50                  55                  60                  


Thr His Asp Leu Ser Gly Tyr Thr Met Tyr Ala Ser Gly Glu Pro Cys 
65                  70                  75                  80  


Pro Met Cys Leu Ser Ala Met Tyr Phe Ala Gly Ile Lys Asp Val Phe 
                85                  90                  95      


Tyr Cys Ala Thr Val Glu Glu Ala Ala Gln Val Gly Leu Glu Lys Ser 
            100                 105                 110         


Lys Asn Val Tyr Asp Asp Leu Gln Lys Ser Lys Gly Glu Arg Ser Leu 
        115                 120                 125             


Val Met Lys Gln Met Pro Leu Glu Asp Asp Gln Glu Asp Pro Met Lys 
    130                 135                 140                 


Leu Trp Asp Glu Arg Thr Asn His Asn Gly Thr Ser 
145                 150                 155     


<210>  182
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.72; 
       tr|A0A378V0W4|A0A378V0W4_MYCFO polypeptide sequence

<400>  182

Met Val His Ala Gln Phe Asp Pro Thr Ala Arg Gln Ala Leu Ala Ala 
1               5                   10                  15      


Thr Ala Val Glu Ala Lys Thr Arg Lys Asp Leu Thr Trp Gln Gln Ile 
            20                  25                  30          


Ala Asp Ala Ala Glu Leu Ser Pro Ala Phe Val Thr Ala Ala Val Leu 
        35                  40                  45              


Gly Gln His Ala Leu Pro Ala Arg Ser Ala Glu Ala Val Ala Ala Leu 
    50                  55                  60                  


Leu Gly Leu Asp Asp Asp Ala Ala Leu Leu Leu Gln Thr Ile Pro Ile 
65                  70                  75                  80  


Arg Gly Ser Ile Pro Gly Gly Ile Pro Thr Asp Pro Thr Ile Tyr Arg 
                85                  90                  95      


Phe Tyr Glu Met Leu Gln Val Tyr Gly Thr Thr Leu Lys Ala Leu Val 
            100                 105                 110         


His Glu Gln Phe Gly Asp Gly Ile Ile Ser Ala Ile Asn Phe Lys Leu 
        115                 120                 125             


Asp Val Arg Lys Val Ala Asp Pro Glu Gly Gly Glu Arg Ala Val Ile 
    130                 135                 140                 


Thr Leu Asp Gly Lys Tyr Leu Pro Pro Asn Pro Phe Asp Arg Val Arg 
145                 150                 155                 160 


Tyr Arg Gly Gly Leu Met Asp Phe Ala Gln Arg Thr Ile Asp Ile Ala 
                165                 170                 175     


Arg Gln Asn Val Ala Glu Gly Gly Arg Pro Phe Ala Thr Val Ile Val 
            180                 185                 190         


Lys Asn Gly Glu Ile Leu Ala Glu Ser Pro Asn Leu Val Ala Gln Thr 
        195                 200                 205             


His Asp Pro Thr Ala His Ala Glu Ile Leu Ala Ile Arg Lys Ala Cys 
    210                 215                 220                 


Thr Arg Ile Gly Thr Glu His Leu Ile Gly Ala Thr Ile Tyr Val Leu 
225                 230                 235                 240 


Ala Gln Pro Cys Pro Met Cys Leu Gly Ser Leu Tyr Tyr Cys Ser Pro 
                245                 250                 255     


Asp Glu Val Val Phe Leu Thr Thr Arg Asp Ala Tyr Glu Pro His Tyr 
            260                 265                 270         


Val Asp Asp Arg Lys Tyr Phe Glu Leu Asn Met Phe Tyr Asp Glu Phe 
        275                 280                 285             


Ala Lys Pro Trp Asp Gln Arg Arg Leu Pro Met Arg Tyr Glu Pro Arg 
    290                 295                 300                 


Asp Ala Ala Val Asp Val Tyr Lys Leu Trp Gln Glu Arg Asn Gly Gly 
305                 310                 315                 320 


Glu Arg Arg Val Pro Gly Ala Pro Thr Ser Thr Arg Pro Gly Lys Asn 
                325                 330                 335     


Pro Arg Gly Glu 
            340 


<210>  183
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.73; 
       tr|I3XF03|I3XF03_RHIFR polypeptide sequence

<400>  183

Met Lys Gln Arg Cys Met Ser Pro Lys Ser Ala Gln Arg Phe Trp Asp 
1               5                   10                  15      


Asn Asp Met His Asn Asn Lys Asp Arg Pro Met Ser Glu Asn Glu Leu 
            20                  25                  30          


Phe Val Ala Ala Arg Glu Ala Met Ala Lys Ala His Ala Pro Tyr Ser 
        35                  40                  45              


Lys Phe Pro Val Gly Ala Ala Ile Arg Ala Glu Asp Gly Gln Ile Tyr 
    50                  55                  60                  


Thr Gly Ala Asn Ile Glu Asn Leu Ser Phe Pro Glu Gly Trp Cys Ala 
65                  70                  75                  80  


Glu Thr Thr Ala Ile Ser His Met Val Met Ala Gly Gln Arg Lys Ile 
                85                  90                  95      


Met Glu Val Ala Val Ile Ala Glu Lys Leu Ala Leu Cys Pro Pro Cys 
            100                 105                 110         


Gly Gly Cys Arg Gln Arg Leu Ala Glu Phe Ser Gly Ala Ser Thr Arg 
        115                 120                 125             


Ile Tyr Leu Cys Asp Glu Thr Gly Ile Lys Lys Ser Leu Ala Leu Ser 
    130                 135                 140                 


Asp Leu Leu Pro His Ser Phe Glu Thr Glu Ile Leu Gly 
145                 150                 155         


<210>  184
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.74; 
       tr|F8IEF3|F8IEF3_ALIAT polypeptide sequence

<400>  184

Met Asp Ala Lys Glu Leu Glu Thr Arg Gly Trp Leu Cys Met Arg Ala 
1               5                   10                  15      


Val Asp Val Ile Asp Lys Lys Arg Arg Gly Glu Ala Leu Ala Glu Glu 
            20                  25                  30          


Glu Leu Arg Phe Leu Ile Glu Gly Tyr Val Ala Gly Arg Ile Pro Asp 
        35                  40                  45              


Tyr Gln Met Ser Ala Phe Leu Met Ala Val Val Trp Arg Gly Met Thr 
    50                  55                  60                  


Arg Glu Glu Thr Leu Val Leu Thr Arg Leu Leu Ala Asp Ser Gly Glu 
65                  70                  75                  80  


Arg Leu Asp Leu Ser Gly Ile Pro Gly Val Lys Val Asp Lys His Ser 
                85                  90                  95      


Thr Gly Gly Val Gly Asp Lys Ala Thr Leu Val Val Leu Pro Leu Val 
            100                 105                 110         


Ala Ser Ile Gly Val Pro Val Ile Lys Met Ser Gly Arg Gly Leu Gly 
        115                 120                 125             


His Thr Gly Gly Thr Ile Asp Lys Leu Glu Ser Ile Pro Gly Phe Arg 
    130                 135                 140                 


Thr Asp Leu Ser Val Ala Glu Leu Val Ala Gln Val Arg Gln Val Gly 
145                 150                 155                 160 


Ile Ala Leu Gly Gly Gln Thr Ala Asp Leu Ala Pro Ala Asp Lys Lys 
                165                 170                 175     


Leu Tyr Ala Leu Arg Asp Val Thr Gly Thr Val Glu Ser Leu Pro Leu 
            180                 185                 190         


Ile Ala Ser Ser Val Met Ser Lys Lys Leu Ala Gly Gly Ala Asp Ala 
        195                 200                 205             


Ile Val Leu Asp Val Lys Val Gly Asp Gly Ala Phe Met Lys Ser Arg 
    210                 215                 220                 


Ser Asp Ala Arg Arg Leu Ala Arg Leu Met Val Glu Ile Gly Glu Ala 
225                 230                 235                 240 


Ala Gly Arg Arg Thr Val Ala Val Leu Ser Asn Met Asp Gln Pro Leu 
                245                 250                 255     


Gly Cys Ala Ile Gly Asn Ala Leu Glu Val Ala Glu Ala Ile Arg Val 
            260                 265                 270         


Leu Ser Gly Glu Gly Pro Phe Asp Leu Ala Glu Ile Ala Leu Ala Leu 
        275                 280                 285             


Ala Glu Glu Met Thr Val Leu Ala Gly Val Ala Ala Thr Arg Glu Glu 
    290                 295                 300                 


Ala Arg Arg Met Leu Arg Gln Ser Val Ala Glu Gly Arg Ala Leu Glu 
305                 310                 315                 320 


Thr Leu Arg Arg Trp Ile Ala Ala Gln Gly Gly Asp Pro Ala Val Val 
                325                 330                 335     


Asp Asp Pro Ser Arg Leu Pro Gln Ala Pro Val Gln Met Pro Tyr Leu 
            340                 345                 350         


Pro Lys Lys Ala Gly Phe Val Ala Lys Leu Ser Ala Leu Ala Phe Gly 
        355                 360                 365             


Leu Ala Ala Met Arg Leu Gly Ala Gly Arg Glu Thr Lys Glu Glu Ala 
    370                 375                 380                 


Ile Asp Pro Ser Val Gly Ile Val Leu His Ala Lys Val Gly Asp Arg 
385                 390                 395                 400 


Val Gln Thr His Arg Pro Met Phe Thr Val His Ala Arg Thr Gly Glu 
                405                 410                 415     


Asp Ala Leu Arg Cys Ile Gln Glu Leu Glu Ala Ala Ile Gln Ile Ser 
            420                 425                 430         


Asp Asp Pro Val Glu Ala Pro Pro Leu Ile Leu Ala Arg Ile Asp Arg 
        435                 440                 445             


Ser Glu Ala Leu Pro Tyr Ala Asp Leu Met Asp Ala Ala Arg Glu Ala 
    450                 455                 460                 


Arg Asp Arg Ala Tyr Val Pro Tyr Ser Gly Phe Ala Val Gly Ala Ala 
465                 470                 475                 480 


Leu Glu Leu Ala Asp Gly Arg Met Val Thr Gly Ala Asn Val Glu Asn 
                485                 490                 495     


Ala Ser Tyr Gly Leu Thr Asn Cys Ala Glu Arg Ser Ala Val Phe Arg 
            500                 505                 510         


Ala Val Ala Glu Gly Gly Pro Gly Thr Lys Pro Glu Ile Arg Ala Val 
        515                 520                 525             


Ala Val Ile Ala Asp Ser Pro Glu Pro Val Ser Pro Cys Gly Ala Cys 
    530                 535                 540                 


Arg Gln Val Leu Ala Glu Phe Cys Ser Pro Asp Thr Pro Val Tyr Leu 
545                 550                 555                 560 


Gly Asn Leu Gln Gly Asp Val Arg Glu Thr Thr Val Gly Ala Leu Leu 
                565                 570                 575     


Pro Gly Ala Phe Thr Asp Ala Gln Met Ala Asn Val Arg Arg Gln Asp 
            580                 585                 590         


Lys Glu Ala 
        595 


<210>  185
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.75; 
       tr|A0A1G3M638|A0A1G3M638_9SPIR polypeptide sequence

<400>  185

Met Lys Thr Thr Asn Ile Asn Ala Leu Asp Lys Trp Asp Leu Arg Phe 
1               5                   10                  15      


Leu Gln Met Ala Glu His Val Ala Glu Trp Ser Lys Asp Pro Ser Thr 
            20                  25                  30          


Lys Val Gly Ala Val Ile Val Arg Pro Asp Arg Thr Ile Ala Ser Val 
        35                  40                  45              


Gly Phe Asn Gly Phe Ala Arg Gly Val Arg Asp Thr Val Glu Arg Leu 
    50                  55                  60                  


Trp Asn Arg Glu Leu Lys Tyr Pro Leu Thr Val His Ala Glu Leu Asn 
65                  70                  75                  80  


Ala Ile Leu Ser Ala His Glu Pro Val Arg Gly His Ser Leu Tyr Val 
                85                  90                  95      


Ser Pro Leu Ser Pro Cys Ser Asn Cys Ala Gly Val Ile Ile Gln Ser 
            100                 105                 110         


Gly Ile Ala Arg Val Val Ala Lys Cys Gly Gln Val Asn Asn Pro Ala 
        115                 120                 125             


Gln Trp Ser Glu Ser Phe Asn Leu Ala Leu Thr Ala Phe Ala Glu Ala 
    130                 135                 140                 


Gly Val Ser Val Ile Leu Val Glu His 
145                 150             


<210>  186
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.76; 
       tr|A0A3D9LFR2|A0A3D9LFR2_9MICC polypeptide sequence

<400>  186

Met Glu Gln Asn Asp His Gly Ser Ser Gly Ala Phe Ser Asp Pro Phe 
1               5                   10                  15      


Glu Asp Asp Ile Pro Leu Thr Ala Ser Leu Pro Arg Ile Thr Gly Thr 
            20                  25                  30          


Gly Ser Gly Ile Asp Trp Gln Arg Leu Glu Ser Thr Ala Arg Ala Ala 
        35                  40                  45              


Met Thr Arg Ala Tyr Val Pro Tyr Ser Arg Phe Pro Val Gly Ala Ala 
    50                  55                  60                  


Ala Leu Val Glu Asp Gly Arg Val Val Ala Gly Cys Asn Ile Glu Asn 
65                  70                  75                  80  


Ala Ser Leu Gly Leu Thr Leu Cys Ala Glu Cys Ser Leu Val Ser Asn 
                85                  90                  95      


Leu Gln Met Ser Gly Gly Gly Arg Ile Val Ala Phe Tyr Cys Val Asp 
            100                 105                 110         


Gly Asn Gly Glu Val Leu Met Pro Cys Gly Arg Cys Arg Gln Leu Leu 
        115                 120                 125             


Tyr Glu Phe His Ala Pro Gly Met Arg Leu Met Gly Pro Asp Gly Glu 
    130                 135                 140                 


Leu Thr Met Asp Glu Val Leu Pro Leu Ala Phe Gly Pro Ala Asp Met 
145                 150                 155                 160 


Thr His Leu Ser Asp Ser Ala Ala Ser Thr Asp Asp Pro Gly Arg Thr 
                165                 170                 175     


Arg 
    


<210>  187
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.77; 
       tr|A0A3B9YGB5|A0A3B9YGB5_9BACT polypeptide sequence

<400>  187

Met Ala Lys Pro Ile Ser Lys Lys Tyr Arg Lys Leu Ile Glu Thr Ala 
1               5                   10                  15      


Lys Ala Ala Arg Lys Lys Ala Tyr Ser Pro Tyr Ser Arg Tyr Gln Val 
            20                  25                  30          


Gly Ala Ala Val Leu Thr Glu Ser Gly Arg Ile Tyr Ser Gly Ala Asn 
        35                  40                  45              


Met Glu Asn Ala Ser Tyr Gly Leu Cys Met Cys Ala Glu Arg Val Ala 
    50                  55                  60                  


Ile Ala Asn Ala Val Thr Arg Gly Glu Lys Val Leu Gln Ala Val Cys 
65                  70                  75                  80  


Val Val Gly Lys Lys Ala Arg Pro Cys Gly Ala Cys Arg Gln Val Met 
                85                  90                  95      


Leu Glu Phe Ser Thr Lys Glu Thr Glu Leu Leu Met Val Asp Ile Asp 
            100                 105                 110         


Pro Asn Ala Arg Arg Asp Thr Val Ile Arg Thr Arg Val Tyr Ser Met 
        115                 120                 125             


Leu Pro Asn Pro Phe Asp Pro Phe Glu Ser Gly Met Leu Pro Gln His 
    130                 135                 140                 


Pro Gln Asn Leu Leu Arg Arg Arg Lys Ser Pro Gln Pro Arg Arg Lys 
145                 150                 155                 160 


Arg Arg Ser Arg Pro Val His Arg Glu Val Ser Arg 
                165                 170         


<210>  188
<211>  391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.78; 
       tr|A0A182F569|A0A182F569_ANOAL polypeptide sequence

<400>  188

Met Pro Arg Pro Ser Gln Phe Arg Val Ser Ser Ser Gln Ser Leu Ser 
1               5                   10                  15      


Asn Ser Gln Ile Gln Ala Ser Gln Ser Ser Asp Ser Val Val Asp Ile 
            20                  25                  30          


Thr Ser Tyr Val Asn Ala Val Val Lys Ala Leu Leu Asn Leu Ser Cys 
        35                  40                  45              


Thr Lys Thr Ile Ile Lys Arg Ala Asp Leu Val Asn Ile Ala Leu Lys 
    50                  55                  60                  


Gly Asn Gly Arg Leu Ile Gly Arg Val Leu Gln Asp Ala Asn Ile Glu 
65                  70                  75                  80  


Leu Lys Glu Ile Tyr Gly Tyr Glu Leu Ile Glu Val Glu Lys Ser Lys 
                85                  90                  95      


Thr Met Ile Leu Cys Ser Thr Leu Ala Ala Gly Ser Met Asp Glu Leu 
            100                 105                 110         


Asn Asp Ala Asn Arg Arg Arg Tyr Thr Phe Leu Tyr Leu Ile Leu Gly 
        115                 120                 125             


Tyr Ile Phe Met Lys Asn Gly Ser Val Pro Glu Thr Ile Val Trp Glu 
    130                 135                 140                 


Phe Leu Glu Thr Leu Gly Ile Glu Glu Gln Gln Glu His Asn Tyr Phe 
145                 150                 155                 160 


Gly Asp Val Arg Lys Leu Tyr Asp Ser Leu Phe Lys Gln Ala Tyr Leu 
                165                 170                 175     


Thr Arg Thr Lys Gln Ala Leu Glu Gly Leu Asn Asp Asp Val Met Leu 
            180                 185                 190         


Ile Ser Trp Gly Val Arg Ser Lys His Glu Val Ser Lys Lys Asp Ile 
        195                 200                 205             


Leu Ala Gly Phe Cys Lys Val Met Asn Arg Asp Pro Val Asp Phe Lys 
    210                 215                 220                 


Ala Gln Tyr Ile Glu Ala Asn Glu Lys Asp Asp Lys Met Asn Asn Asn 
225                 230                 235                 240 


Ile Asn Gly Thr Val Asp Gly Arg Asn Thr Val Glu Tyr Ser Ser Leu 
                245                 250                 255     


Asp Ala Ser Val Lys Glu Leu Ile Glu Ala Ala Ile Lys Val Arg Asn 
            260                 265                 270         


Asn Ala Tyr Cys Pro Tyr Ser Asn Phe Ala Val Gly Ala Ala Leu Arg 
        275                 280                 285             


Thr Val Gly Gly Asp Ile Val Thr Gly Cys Asn Val Glu Asn Gly Thr 
    290                 295                 300                 


Phe Gly Pro Ser Val Cys Ala Glu Arg Thr Ala Val Cys Lys Ala Val 
305                 310                 315                 320 


Ser Glu Gly His Arg Glu Phe Thr Ala Val Ala Val Val Ala Phe Gln 
                325                 330                 335     


Glu Thr Glu Phe Thr Ala Pro Cys Gly Thr Cys Arg Gln Thr Leu Ser 
            340                 345                 350         


Glu Phe Ser Arg Lys Asp Ile Pro Ile Tyr Leu Val Lys Pro Ser Pro 
        355                 360                 365             


Val Arg Val Met Val Thr Ser Leu Phe Gln Leu Leu Pro His Ala Phe 
    370                 375                 380                 


Ser Pro Ser Phe Leu Asn Lys 
385                 390     


<210>  189
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.79; 
       tr|A0A264Z0D4|A0A264Z0D4_9BACI polypeptide sequence

<400>  189

Met Glu Pro Lys Lys Leu Ile Glu Glu Ala Ile Val Ala Ser Lys Gln 
1               5                   10                  15      


Ala Tyr Val Gln Tyr Ser Asn Phe His Val Gly Ala Ala Leu Leu Thr 
            20                  25                  30          


Lys Asp Gly Lys Leu Tyr His Gly Cys Asn Ile Glu Asn Ala Ser Tyr 
        35                  40                  45              


Gly Leu Thr Asn Cys Ala Glu Arg Thr Ala Ile Phe Lys Ala Val Ser 
    50                  55                  60                  


Glu Gly Glu Lys Glu Phe Gln Ala Ile Ala Val Val Gly Asp Thr Glu 
65                  70                  75                  80  


Gly Pro Ile Ser Pro Cys Gly Ala Cys Arg Gln Val Leu Ala Glu Phe 
                85                  90                  95      


Phe Ser Pro Asp Thr Val Val Ile Leu Ala Asn Leu Lys Gly Asp His 
            100                 105                 110         


Val Val Thr Asn Ile Asn Glu Leu Leu Pro Gly Phe Phe Ser Ser Lys 
        115                 120                 125             


Asp Leu Gln Lys Lys Val Lys Asn Cys Phe Glu Lys Asn Ala Leu Gly 
    130                 135                 140                 


Ser Ser Cys Leu Arg Pro Ile 
145                 150     


<210>  190
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pYY-BEM4.80; 
       tr|A0A1L9Q1R3|A0A1L9Q1R3_ASPVE polypeptide sequence

<400>  190

Met Pro Leu Ser Ala Glu Glu Ala Ala Leu Val Glu Thr Ala Thr Ala 
1               5                   10                  15      


Thr Ile Asn Ser Ile Pro Leu Ser Glu Asp Tyr Ser Val Ala Ser Ala 
            20                  25                  30          


Ala Lys Ala Ser Asp Gly Arg Val Phe Thr Gly Val Asn Val Tyr His 
        35                  40                  45              


Phe Thr Gly Gly Pro Cys Ala Glu Leu Val Val Leu Gly Val Ala Ala 
    50                  55                  60                  


Ala Ala Gly Ala Ala Gln Leu Thr His Ile Val Ala Val Ala Asn Glu 
65                  70                  75                  80  


Gln Arg Gly Ile Leu Ser Pro Cys Gly Arg Cys Arg Gln Val Leu Leu 
                85                  90                  95      


Asp Leu Gln Pro Asn Ile Gln Val Ile Val Gly Lys Glu Gly Ser Glu 
            100                 105                 110         


Gln Ser Val Pro Val Ala Gln Leu Leu Pro Phe Ser Tyr Arg Gln Pro 
        115                 120                 125             


Asp Gln His Thr Pro Val Ile Phe Lys Ala Leu Thr Ser Ser Gly Pro 
    130                 135                 140                 


Val Val Val Asp Phe Phe Ala Thr Trp Cys Gly Pro Cys Lys Ala Val 
145                 150                 155                 160 


Ala Pro Val Val Gly Lys Leu Ser Glu Thr Tyr Thr Asp Val Arg Phe 
                165                 170                 175     


Ile Gln Val Asp Val Asp Lys Ala Arg Ser Ile Ser Gln Glu His Asp 
            180                 185                 190         


Ile Arg Ala Met Pro Thr Phe Val Leu Tyr Lys Asp Gly Lys Leu Leu 
        195                 200                 205             


Asp Lys Arg Val Val Gly Gly Asn Met Lys Glu Leu Glu Glu Gln Ile 
    210                 215                 220                 


Lys Ala Ile Ile Ala 
225                 


<210>  191
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  191

Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg 
1               5                   10                  15      


Lys Val 
        


<210>  192
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  192

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  193
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  193

Lys Lys Thr Glu Leu Gln Thr Thr Asn Ala Glu Asn Lys Thr Lys Lys 
1               5                   10                  15      


Leu 
    


<210>  194
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  194

Lys Arg Gly Ile Asn Asp Arg Asn Phe Trp Arg Gly Glu Asn Gly Arg 
1               5                   10                  15      


Lys Thr Arg 
            


<210>  195
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  195

Arg Lys Ser Gly Lys Ile Ala Ala Ile Val Val Lys Arg Pro Arg Lys 
1               5                   10                  15      


<210>  196
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide

<400>  196

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  197
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide

<400>  197

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys 
            20                  25                  30  


<210>  198
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes


<220>
<221>  MISC_FEATURE
<222>  (1)..(1368)
<223>  Exemplary PAM-binding SpCas9 polypeptide sequence

<220>
<221>  MISC_FEATURE
<222>  (5)..(58)
<223>  RuvC domain

<220>
<221>  MISC_FEATURE
<222>  (718)..(764)
<223>  RuvC domain

<220>
<221>  MISC_FEATURE
<222>  (773)..(902)
<223>  HNH domain

<400>  198

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  199
<211>  4104
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(4104)
<223>  wild type spCas9 polynucleotide sequence

<400>  199
atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg       60

atcactgatg attataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc      120

cacagtatca aaaaaaatct tataggggct cttttatttg gcagtggaga gacagcggaa      180

gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt      240

tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga      300

cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga      360

aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa      420

aaattggcag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat      480

atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat      540

gtggacaaac tatttatcca gttggtacaa atctacaatc aattatttga agaaaaccct      600

attaacgcaa gtagagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga      660

cgattagaaa atctcattgc tcagctcccc ggtgagaaga gaaatggctt gtttgggaat      720

ctcattgctt tgtcattggg attgacccct aattttaaat caaattttga tttggcagaa      780

gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg      840

caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt      900

ttactttcag atatcctaag agtaaatagt gaaataacta aggctcccct atcagcttca      960

atgattaagc gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga     1020

caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca     1080

ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta     1140

gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc     1200

aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat     1260

gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt     1320

gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt     1380

cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa     1440

gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa     1500

aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt     1560

tataacgaat tgacaaaggt caaatatgtt actgagggaa tgcgaaaacc agcatttctt     1620

tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc     1680

gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt     1740

tcaggagttg aagatagatt taatgcttca ttaggcgcct accatgattt gctaaaaatt     1800

attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt     1860

ttaacattga ccttatttga agataggggg atgattgagg aaagacttaa aacatatgct     1920

cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga     1980

cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta     2040

gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat     2100

agtttgacat ttaaagaaga tattcaaaaa gcacaggtgt ctggacaagg ccatagttta     2160

catgaacaga ttgctaactt agctggcagt cctgctatta aaaaaggtat tttacagact     2220

gtaaaaattg ttgatgaact ggtcaaagta atggggcata agccagaaaa tatcgttatt     2280

gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg     2340

aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt     2400

gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctacaaaa tggaagagac     2460

atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt     2520

gttccacaaa gtttcattaa agacgattca atagacaata aggtactaac gcgttctgat     2580

aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac     2640

tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg     2700

aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg     2760

gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact     2820

aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa     2880

ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac     2940

catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat     3000

ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg     3060

attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat     3120

atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct     3180

ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc     3240

acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag     3300

acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct     3360

cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat     3420

tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa     3480

gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt     3540

ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat     3600

agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa     3660

aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat     3720

tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag     3780

cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt     3840

ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca     3900

atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc     3960

gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa     4020

gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat     4080

ttgagtcagc taggaggtga ctga                                            4104


<210>  200
<211>  1367
<212>  PRT
<213>  Streptococcus pyogenes


<220>
<221>  MISC_FEATURE
<222>  (1)..(1367)
<223>  spCas9 polypeptide sequence

<400>  200

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Gly Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Ile Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Ala Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Gly Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1100                 1105                 1110             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1145                 1150                 1155             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1160                 1165                 1170             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1175                 1180                 1185             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1190                 1195                 1200             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1205                 1210                 1215             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1220                 1225                 1230             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1235                 1240                 1245             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1250                 1255                 1260             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1265                 1270                 1275             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1280                 1285                 1290             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1295                 1300                 1305             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1325                 1330                 1335             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  201
<211>  4212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: wild-type Cas9 polynucleotide
       sequence


<220>
<221>  misc_feature
<222>  (5)..(58)
<223>  RuvC domain

<220>
<221>  misc_feature
<222>  (718)..(764)
<223>  RuvC domain

<220>
<221>  misc_feature
<222>  (772)..(901)
<223>  HNH domain

<220>
<221>  misc_feature
<222>  (906)..(1101)
<223>  RuvC domain

<400>  201
atggataaaa agtattctat tggtttagac atcggcacta attccgttgg atgggctgtc       60

ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt      120

cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag      180

gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt      240

tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt      300

ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga      360

aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa      420

aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat      480

atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat      540

gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct      600

ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga      660

cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac      720

cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa      780

gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca      840

caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc      900

ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca      960

atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt     1020

cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca     1080

ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta     1140

gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga     1200

aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat     1260

gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt     1320

gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct     1380

cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa     1440

gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag     1500

aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg     1560

tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta     1620

agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca     1680

gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc     1740

tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata     1800

attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg     1860

ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct     1920

cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga     1980

cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc     2040

gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac     2100

tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg     2160

cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca     2220

gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta     2280

atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg     2340

atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct     2400

gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg     2460

gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac     2520

attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg     2580

gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag     2640

aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta     2700

actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag     2760

ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat     2820

acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca     2880

aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac     2940

taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa     3000

tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag     3060

atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct     3120

aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga     3180

cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc     3240

gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg     3300

cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc     3360

gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc     3420

tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc     3480

aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac     3540

ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag     3600

tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt     3660

caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc     3720

cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag     3780

cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc     3840

atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa     3900

cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct     3960

ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag     4020

gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata     4080

gatttgtcac agcttggggg tgacggatcc cccaagaaga agaggaaagt ctcgagcgac     4140

tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac     4200

aaggctgcag ga                                                         4212


<210>  202
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: exemplary catalytically 
       active Cas9 nickase (nCas9) polypeptide sequence

<400>  202

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  203
<211>  4107
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(4107)
<223>  Cas9 from Streptococcus pyogenes (NCBI Ref. Seq.: NC_002737.2) 
       polynucleotide sequence

<400>  203
atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg       60

atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc      120

cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa      180

gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt      240

tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga      300

cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga      360

aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa      420

aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat      480

atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat      540

gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct      600

attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga      660

cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat      720

ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa      780

gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg      840

caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt      900

ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca      960

atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga     1020

caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca     1080

ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta     1140

gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc     1200

aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat     1260

gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt     1320

gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt     1380

cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa     1440

gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa     1500

aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt     1560

tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt     1620

tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc     1680

gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt     1740

tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt     1800

attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt     1860

ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct     1920

cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga     1980

cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta     2040

gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat     2100

agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta     2160

catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact     2220

gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt     2280

attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt     2340

atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct     2400

gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga     2460

gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac     2520

attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct     2580

gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa     2640

aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta     2700

acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa     2760

ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat     2820

actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct     2880

aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat     2940

taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa     3000

tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa     3060

atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct     3120

aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc     3180

cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt     3240

gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta     3300

cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt     3360

gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct     3420

tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt     3480

aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac     3540

tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa     3600

tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta     3660

caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt     3720

cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag     3780

cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt     3840

attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa     3900

ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct     3960

cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa     4020

gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt     4080

gatttgagtc agctaggagg tgactga                                         4107


<210>  204
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of sequence: catalytically inactive Cas9 (dCas9) 
       polypeptide sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(58)
<223>  RuvC domain

<220>
<221>  MISC_FEATURE
<222>  (718)..(764)
<223>  RuvC domain

<220>
<221>  MISC_FEATURE
<222>  (773)..(902)
<223>  HNH domain

<220>
<221>  MISC_FEATURE
<222>  (907)..(1102)
<223>  RuvC domain

<400>  204

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  205
<211>  345
<212>  PRT
<213>  Sulfolobus islandicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(345)
<223>  tr|F0NN87|F0NN87_SULIHCRISPR-associatedCasx protein OS = 
       Sulfolobus islandicus (strain HVE10/4) GN = SiH_0402 PE=4 SV=1); 
       CasX polypeptide sequence

<400>  205

Met Glu Val Pro Leu Tyr Asn Ile Phe Gly Asp Asn Tyr Ile Ile Gln 
1               5                   10                  15      


Val Ala Thr Glu Ala Glu Asn Ser Thr Ile Tyr Asn Asn Lys Val Glu 
            20                  25                  30          


Ile Asp Asp Glu Glu Leu Arg Asn Val Leu Asn Leu Ala Tyr Lys Ile 
        35                  40                  45              


Ala Lys Asn Asn Glu Asp Ala Ala Ala Glu Arg Arg Gly Lys Ala Lys 
    50                  55                  60                  


Lys Lys Lys Gly Glu Glu Gly Glu Thr Thr Thr Ser Asn Ile Ile Leu 
65                  70                  75                  80  


Pro Leu Ser Gly Asn Asp Lys Asn Pro Trp Thr Glu Thr Leu Lys Cys 
                85                  90                  95      


Tyr Asn Phe Pro Thr Thr Val Ala Leu Ser Glu Val Phe Lys Asn Phe 
            100                 105                 110         


Ser Gln Val Lys Glu Cys Glu Glu Val Ser Ala Pro Ser Phe Val Lys 
        115                 120                 125             


Pro Glu Phe Tyr Glu Phe Gly Arg Ser Pro Gly Met Val Glu Arg Thr 
    130                 135                 140                 


Arg Arg Val Lys Leu Glu Val Glu Pro His Tyr Leu Ile Ile Ala Ala 
145                 150                 155                 160 


Ala Gly Trp Val Leu Thr Arg Leu Gly Lys Ala Lys Val Ser Glu Gly 
                165                 170                 175     


Asp Tyr Val Gly Val Asn Val Phe Thr Pro Thr Arg Gly Ile Leu Tyr 
            180                 185                 190         


Ser Leu Ile Gln Asn Val Asn Gly Ile Val Pro Gly Ile Lys Pro Glu 
        195                 200                 205             


Thr Ala Phe Gly Leu Trp Ile Ala Arg Lys Val Val Ser Ser Val Thr 
    210                 215                 220                 


Asn Pro Asn Val Ser Val Val Arg Ile Tyr Thr Ile Ser Asp Ala Val 
225                 230                 235                 240 


Gly Gln Asn Pro Thr Thr Ile Asn Gly Gly Phe Ser Ile Asp Leu Thr 
                245                 250                 255     


Lys Leu Leu Glu Lys Arg Tyr Leu Leu Ser Glu Arg Leu Glu Ala Ile 
            260                 265                 270         


Ala Arg Asn Ala Leu Ser Ile Ser Ser Asn Met Arg Glu Arg Tyr Ile 
        275                 280                 285             


Val Leu Ala Asn Tyr Ile Tyr Glu Tyr Leu Thr Gly Ser Lys Arg Leu 
    290                 295                 300                 


Glu Asp Leu Leu Tyr Phe Ala Asn Arg Asp Leu Ile Met Asn Leu Asn 
305                 310                 315                 320 


Ser Asp Asp Gly Lys Val Arg Asp Leu Lys Leu Ile Ser Ala Tyr Val 
                325                 330                 335     


Asn Gly Glu Leu Ile Arg Gly Glu Gly 
            340                 345 


<210>  206
<211>  345
<212>  PRT
<213>  Sulfolobus islandicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(345)
<223>  tr|F0NH53|F0NH53_SULIR CRISPR associated protein, Casx OS = 
       Sulfolobus islandicus (strain REY15A) GN=SiRe_0771 PE=4 SV=1); 
       CasX polypeptide sequence

<400>  206

Met Glu Val Pro Leu Tyr Asn Ile Phe Gly Asp Asn Tyr Ile Ile Gln 
1               5                   10                  15      


Val Ala Thr Glu Ala Glu Asn Ser Thr Ile Tyr Asn Asn Lys Val Glu 
            20                  25                  30          


Ile Asp Asp Glu Glu Leu Arg Asn Val Leu Asn Leu Ala Tyr Lys Ile 
        35                  40                  45              


Ala Lys Asn Asn Glu Asp Ala Ala Ala Glu Arg Arg Gly Lys Ala Lys 
    50                  55                  60                  


Lys Lys Lys Gly Glu Glu Gly Glu Thr Thr Thr Ser Asn Ile Ile Leu 
65                  70                  75                  80  


Pro Leu Ser Gly Asn Asp Lys Asn Pro Trp Thr Glu Thr Leu Lys Cys 
                85                  90                  95      


Tyr Asn Phe Pro Thr Thr Val Ala Leu Ser Glu Val Phe Lys Asn Phe 
            100                 105                 110         


Ser Gln Val Lys Glu Cys Glu Glu Val Ser Ala Pro Ser Phe Val Lys 
        115                 120                 125             


Pro Glu Phe Tyr Lys Phe Gly Arg Ser Pro Gly Met Val Glu Arg Thr 
    130                 135                 140                 


Arg Arg Val Lys Leu Glu Val Glu Pro His Tyr Leu Ile Met Ala Ala 
145                 150                 155                 160 


Ala Gly Trp Val Leu Thr Arg Leu Gly Lys Ala Lys Val Ser Glu Gly 
                165                 170                 175     


Asp Tyr Val Gly Val Asn Val Phe Thr Pro Thr Arg Gly Ile Leu Tyr 
            180                 185                 190         


Ser Leu Ile Gln Asn Val Asn Gly Ile Val Pro Gly Ile Lys Pro Glu 
        195                 200                 205             


Thr Ala Phe Gly Leu Trp Ile Ala Arg Lys Val Val Ser Ser Val Thr 
    210                 215                 220                 


Asn Pro Asn Val Ser Val Val Ser Ile Tyr Thr Ile Ser Asp Ala Val 
225                 230                 235                 240 


Gly Gln Asn Pro Thr Thr Ile Asn Gly Gly Phe Ser Ile Asp Leu Thr 
                245                 250                 255     


Lys Leu Leu Glu Lys Arg Asp Leu Leu Ser Glu Arg Leu Glu Ala Ile 
            260                 265                 270         


Ala Arg Asn Ala Leu Ser Ile Ser Ser Asn Met Arg Glu Arg Tyr Ile 
        275                 280                 285             


Val Leu Ala Asn Tyr Ile Tyr Glu Tyr Leu Thr Gly Ser Lys Arg Leu 
    290                 295                 300                 


Glu Asp Leu Leu Tyr Phe Ala Asn Arg Asp Leu Ile Met Asn Leu Asn 
305                 310                 315                 320 


Ser Asp Asp Gly Lys Val Arg Asp Leu Lys Leu Ile Ser Ala Tyr Val 
                325                 330                 335     


Asn Gly Glu Leu Ile Arg Gly Glu Gly 
            340                 345 


<210>  207
<211>  987
<212>  PRT
<213>  Deltaproteobacteria


<220>
<221>  MISC_FEATURE
<222>  (1)..(987)
<223>  CasX polypeptide sequence

<400>  207

Met Glu Lys Arg Ile Asn Lys Ile Arg Lys Lys Leu Ser Ala Asp Asn 
1               5                   10                  15      


Ala Thr Lys Pro Val Ser Arg Ser Gly Pro Met Lys Thr Leu Leu Val 
            20                  25                  30          


Arg Val Met Thr Asp Asp Leu Lys Lys Arg Leu Glu Lys Arg Arg Lys 
        35                  40                  45              


Lys Pro Glu Val Met Pro Gln Val Ile Ser Asn Asn Ala Ala Asn Asn 
    50                  55                  60                  


Leu Arg Met Leu Leu Asp Asp Tyr Thr Lys Met Lys Glu Ala Ile Leu 
65                  70                  75                  80  


Gln Val Tyr Trp Gln Glu Phe Lys Asp Asp His Val Gly Leu Met Cys 
                85                  90                  95      


Lys Phe Ala Gln Pro Ala Ser Lys Lys Ile Asp Gln Asn Lys Leu Lys 
            100                 105                 110         


Pro Glu Met Asp Glu Lys Gly Asn Leu Thr Thr Ala Gly Phe Ala Cys 
        115                 120                 125             


Ser Gln Cys Gly Gln Pro Leu Phe Val Tyr Lys Leu Glu Gln Val Ser 
    130                 135                 140                 


Glu Lys Gly Lys Ala Tyr Thr Asn Tyr Phe Gly Arg Cys Asn Val Ala 
145                 150                 155                 160 


Glu His Glu Lys Leu Ile Leu Leu Ala Gln Leu Lys Pro Val Lys Asp 
                165                 170                 175     


Ser Asp Glu Ala Val Thr Tyr Ser Leu Gly Lys Phe Gly Gln Arg Ala 
            180                 185                 190         


Leu Asp Phe Tyr Ser Ile His Val Thr Lys Glu Ser Thr His Pro Val 
        195                 200                 205             


Lys Pro Leu Ala Gln Ile Ala Gly Asn Arg Tyr Ala Ser Gly Pro Val 
    210                 215                 220                 


Gly Lys Ala Leu Ser Asp Ala Cys Met Gly Thr Ile Ala Ser Phe Leu 
225                 230                 235                 240 


Ser Lys Tyr Gln Asp Ile Ile Ile Glu His Gln Lys Val Val Lys Gly 
                245                 250                 255     


Asn Gln Lys Arg Leu Glu Ser Leu Arg Glu Leu Ala Gly Lys Glu Asn 
            260                 265                 270         


Leu Glu Tyr Pro Ser Val Thr Leu Pro Pro Gln Pro His Thr Lys Glu 
        275                 280                 285             


Gly Val Asp Phe Ala Tyr Asn Glu Val Ile Ala Arg Val Arg Met Trp 
    290                 295                 300                 


Val Asn Leu Asn Leu Trp Gln Lys Leu Lys Leu Ser Arg Asp Asp Ala 
305                 310                 315                 320 


Lys Pro Leu Leu Arg Leu Lys Gly Phe Pro Ser Phe Pro Val Val Glu 
                325                 330                 335     


Arg Arg Glu Asn Glu Val Asp Trp Trp Asn Thr Ile Asn Glu Val Lys 
            340                 345                 350         


Lys Leu Ile Asp Ala Lys Arg Asp Met Gly Arg Val Phe Trp Ser Gly 
        355                 360                 365             


Val Thr Ala Glu Lys Arg Asn Thr Ile Leu Glu Gly Tyr Asn Tyr Leu 
    370                 375                 380                 


Pro Asn Glu Asn Asp His Lys Lys Arg Glu Gly Ser Leu Glu Asn Pro 
385                 390                 395                 400 


Lys Lys Pro Ala Lys Arg Gln Phe Gly Asp Leu Leu Leu Tyr Leu Glu 
                405                 410                 415     


Lys Lys Tyr Ala Gly Asp Trp Gly Lys Val Phe Asp Glu Ala Trp Glu 
            420                 425                 430         


Arg Ile Asp Lys Lys Ile Ala Gly Leu Thr Ser His Ile Glu Arg Glu 
        435                 440                 445             


Glu Ala Arg Asn Ala Glu Asp Ala Gln Ser Lys Ala Val Leu Thr Asp 
    450                 455                 460                 


Trp Leu Arg Ala Lys Ala Ser Phe Val Leu Glu Arg Leu Lys Glu Met 
465                 470                 475                 480 


Asp Glu Lys Glu Phe Tyr Ala Cys Glu Ile Gln Leu Gln Lys Trp Tyr 
                485                 490                 495     


Gly Asp Leu Arg Gly Asn Pro Phe Ala Val Glu Ala Glu Asn Arg Val 
            500                 505                 510         


Val Asp Ile Ser Gly Phe Ser Ile Gly Ser Asp Gly His Ser Ile Gln 
        515                 520                 525             


Tyr Arg Asn Leu Leu Ala Trp Lys Tyr Leu Glu Asn Gly Lys Arg Glu 
    530                 535                 540                 


Phe Tyr Leu Leu Met Asn Tyr Gly Lys Lys Gly Arg Ile Arg Phe Thr 
545                 550                 555                 560 


Asp Gly Thr Asp Ile Lys Lys Ser Gly Lys Trp Gln Gly Leu Leu Tyr 
                565                 570                 575     


Gly Gly Gly Lys Ala Lys Val Ile Asp Leu Thr Phe Asp Pro Asp Asp 
            580                 585                 590         


Glu Gln Leu Ile Ile Leu Pro Leu Ala Phe Gly Thr Arg Gln Gly Arg 
        595                 600                 605             


Glu Phe Ile Trp Asn Asp Leu Leu Ser Leu Glu Thr Gly Leu Ile Lys 
    610                 615                 620                 


Leu Ala Asn Gly Arg Val Ile Glu Lys Thr Ile Tyr Asn Lys Lys Ile 
625                 630                 635                 640 


Gly Arg Asp Glu Pro Ala Leu Phe Val Ala Leu Thr Phe Glu Arg Arg 
                645                 650                 655     


Glu Val Val Asp Pro Ser Asn Ile Lys Pro Val Asn Leu Ile Gly Val 
            660                 665                 670         


Ala Arg Gly Glu Asn Ile Pro Ala Val Ile Ala Leu Thr Asp Pro Glu 
        675                 680                 685             


Gly Cys Pro Leu Pro Glu Phe Lys Asp Ser Ser Gly Gly Pro Thr Asp 
    690                 695                 700                 


Ile Leu Arg Ile Gly Glu Gly Tyr Lys Glu Lys Gln Arg Ala Ile Gln 
705                 710                 715                 720 


Ala Ala Lys Glu Val Glu Gln Arg Arg Ala Gly Gly Tyr Ser Arg Lys 
                725                 730                 735     


Phe Ala Ser Lys Ser Arg Asn Leu Ala Asp Asp Met Val Arg Asn Ser 
            740                 745                 750         


Ala Arg Asp Leu Phe Tyr His Ala Val Thr His Asp Ala Val Leu Val 
        755                 760                 765             


Phe Ala Asn Leu Ser Arg Gly Phe Gly Arg Gln Gly Lys Arg Thr Phe 
    770                 775                 780                 


Met Thr Glu Arg Gln Tyr Thr Lys Met Glu Asp Trp Leu Thr Ala Lys 
785                 790                 795                 800 


Leu Ala Tyr Glu Gly Leu Thr Ser Lys Thr Tyr Leu Ser Lys Thr Leu 
                805                 810                 815     


Ala Gln Tyr Thr Ser Lys Thr Cys Ser Asn Cys Gly Phe Thr Ile Thr 
            820                 825                 830         


Tyr Ala Asp Met Asp Val Met Leu Val Arg Leu Lys Lys Thr Ser Asp 
        835                 840                 845             


Gly Trp Ala Thr Thr Leu Asn Asn Lys Glu Leu Lys Ala Glu Tyr Gln 
    850                 855                 860                 


Ile Thr Tyr Tyr Asn Arg Tyr Lys Arg Gln Thr Val Glu Lys Glu Leu 
865                 870                 875                 880 


Ser Ala Glu Leu Asp Arg Leu Ser Glu Glu Ser Gly Asn Asn Asp Ile 
                885                 890                 895     


Ser Lys Trp Thr Lys Gly Arg Arg Asp Glu Ala Leu Phe Leu Leu Lys 
            900                 905                 910         


Lys Arg Phe Ser His Arg Pro Val Gln Glu Gln Phe Val Cys Leu Asp 
        915                 920                 925             


Cys Gly His Glu Val His Ala Ala Glu Gln Ala Ala Leu Asn Ile Ala 
    930                 935                 940                 


Arg Ser Trp Leu Phe Leu Asn Ser Asn Ser Thr Glu Phe Lys Ser Tyr 
945                 950                 955                 960 


Lys Ser Gly Lys Gln Pro Phe Val Gly Ala Trp Gln Ala Phe Tyr Lys 
                965                 970                 975     


Arg Arg Leu Lys Glu Val Trp Lys Pro Asn Ala 
            980                 985         


<210>  208
<211>  1210
<212>  PRT
<213>  Parcubacteria group bacterium


<220>
<221>  MISC_FEATURE
<222>  (1)..(1210)
<223>  APG80656.1 CRISPR-associated protein CasY [uncultured 
       Parcubacteria group bacterium]; CasY polypeptide sequence

<400>  208

Met Ser Lys Arg His Pro Arg Ile Ser Gly Val Lys Gly Tyr Arg Leu 
1               5                   10                  15      


His Ala Gln Arg Leu Glu Tyr Thr Gly Lys Ser Gly Ala Met Arg Thr 
            20                  25                  30          


Ile Lys Tyr Pro Leu Tyr Ser Ser Pro Ser Gly Gly Arg Thr Val Pro 
        35                  40                  45              


Arg Glu Ile Val Ser Ala Ile Asn Asp Asp Tyr Val Gly Leu Tyr Gly 
    50                  55                  60                  


Leu Ser Asn Phe Asp Asp Leu Tyr Asn Ala Glu Lys Arg Asn Glu Glu 
65                  70                  75                  80  


Lys Val Tyr Ser Val Leu Asp Phe Trp Tyr Asp Cys Val Gln Tyr Gly 
                85                  90                  95      


Ala Val Phe Ser Tyr Thr Ala Pro Gly Leu Leu Lys Asn Val Ala Glu 
            100                 105                 110         


Val Arg Gly Gly Ser Tyr Glu Leu Thr Lys Thr Leu Lys Gly Ser His 
        115                 120                 125             


Leu Tyr Asp Glu Leu Gln Ile Asp Lys Val Ile Lys Phe Leu Asn Lys 
    130                 135                 140                 


Lys Glu Ile Ser Arg Ala Asn Gly Ser Leu Asp Lys Leu Lys Lys Asp 
145                 150                 155                 160 


Ile Ile Asp Cys Phe Lys Ala Glu Tyr Arg Glu Arg His Lys Asp Gln 
                165                 170                 175     


Cys Asn Lys Leu Ala Asp Asp Ile Lys Asn Ala Lys Lys Asp Ala Gly 
            180                 185                 190         


Ala Ser Leu Gly Glu Arg Gln Lys Lys Leu Phe Arg Asp Phe Phe Gly 
        195                 200                 205             


Ile Ser Glu Gln Ser Glu Asn Asp Lys Pro Ser Phe Thr Asn Pro Leu 
    210                 215                 220                 


Asn Leu Thr Cys Cys Leu Leu Pro Phe Asp Thr Val Asn Asn Asn Arg 
225                 230                 235                 240 


Asn Arg Gly Glu Val Leu Phe Asn Lys Leu Lys Glu Tyr Ala Gln Lys 
                245                 250                 255     


Leu Asp Lys Asn Glu Gly Ser Leu Glu Met Trp Glu Tyr Ile Gly Ile 
            260                 265                 270         


Gly Asn Ser Gly Thr Ala Phe Ser Asn Phe Leu Gly Glu Gly Phe Leu 
        275                 280                 285             


Gly Arg Leu Arg Glu Asn Lys Ile Thr Glu Leu Lys Lys Ala Met Met 
    290                 295                 300                 


Asp Ile Thr Asp Ala Trp Arg Gly Gln Glu Gln Glu Glu Glu Leu Glu 
305                 310                 315                 320 


Lys Arg Leu Arg Ile Leu Ala Ala Leu Thr Ile Lys Leu Arg Glu Pro 
                325                 330                 335     


Lys Phe Asp Asn His Trp Gly Gly Tyr Arg Ser Asp Ile Asn Gly Lys 
            340                 345                 350         


Leu Ser Ser Trp Leu Gln Asn Tyr Ile Asn Gln Thr Val Lys Ile Lys 
        355                 360                 365             


Glu Asp Leu Lys Gly His Lys Lys Asp Leu Lys Lys Ala Lys Glu Met 
    370                 375                 380                 


Ile Asn Arg Phe Gly Glu Ser Asp Thr Lys Glu Glu Ala Val Val Ser 
385                 390                 395                 400 


Ser Leu Leu Glu Ser Ile Glu Lys Ile Val Pro Asp Asp Ser Ala Asp 
                405                 410                 415     


Asp Glu Lys Pro Asp Ile Pro Ala Ile Ala Ile Tyr Arg Arg Phe Leu 
            420                 425                 430         


Ser Asp Gly Arg Leu Thr Leu Asn Arg Phe Val Gln Arg Glu Asp Val 
        435                 440                 445             


Gln Glu Ala Leu Ile Lys Glu Arg Leu Glu Ala Glu Lys Lys Lys Lys 
    450                 455                 460                 


Pro Lys Lys Arg Lys Lys Lys Ser Asp Ala Glu Asp Glu Lys Glu Thr 
465                 470                 475                 480 


Ile Asp Phe Lys Glu Leu Phe Pro His Leu Ala Lys Pro Leu Lys Leu 
                485                 490                 495     


Val Pro Asn Phe Tyr Gly Asp Ser Lys Arg Glu Leu Tyr Lys Lys Tyr 
            500                 505                 510         


Lys Asn Ala Ala Ile Tyr Thr Asp Ala Leu Trp Lys Ala Val Glu Lys 
        515                 520                 525             


Ile Tyr Lys Ser Ala Phe Ser Ser Ser Leu Lys Asn Ser Phe Phe Asp 
    530                 535                 540                 


Thr Asp Phe Asp Lys Asp Phe Phe Ile Lys Arg Leu Gln Lys Ile Phe 
545                 550                 555                 560 


Ser Val Tyr Arg Arg Phe Asn Thr Asp Lys Trp Lys Pro Ile Val Lys 
                565                 570                 575     


Asn Ser Phe Ala Pro Tyr Cys Asp Ile Val Ser Leu Ala Glu Asn Glu 
            580                 585                 590         


Val Leu Tyr Lys Pro Lys Gln Ser Arg Ser Arg Lys Ser Ala Ala Ile 
        595                 600                 605             


Asp Lys Asn Arg Val Arg Leu Pro Ser Thr Glu Asn Ile Ala Lys Ala 
    610                 615                 620                 


Gly Ile Ala Leu Ala Arg Glu Leu Ser Val Ala Gly Phe Asp Trp Lys 
625                 630                 635                 640 


Asp Leu Leu Lys Lys Glu Glu His Glu Glu Tyr Ile Asp Leu Ile Glu 
                645                 650                 655     


Leu His Lys Thr Ala Leu Ala Leu Leu Leu Ala Val Thr Glu Thr Gln 
            660                 665                 670         


Leu Asp Ile Ser Ala Leu Asp Phe Val Glu Asn Gly Thr Val Lys Asp 
        675                 680                 685             


Phe Met Lys Thr Arg Asp Gly Asn Leu Val Leu Glu Gly Arg Phe Leu 
    690                 695                 700                 


Glu Met Phe Ser Gln Ser Ile Val Phe Ser Glu Leu Arg Gly Leu Ala 
705                 710                 715                 720 


Gly Leu Met Ser Arg Lys Glu Phe Ile Thr Arg Ser Ala Ile Gln Thr 
                725                 730                 735     


Met Asn Gly Lys Gln Ala Glu Leu Leu Tyr Ile Pro His Glu Phe Gln 
            740                 745                 750         


Ser Ala Lys Ile Thr Thr Pro Lys Glu Met Ser Arg Ala Phe Leu Asp 
        755                 760                 765             


Leu Ala Pro Ala Glu Phe Ala Thr Ser Leu Glu Pro Glu Ser Leu Ser 
    770                 775                 780                 


Glu Lys Ser Leu Leu Lys Leu Lys Gln Met Arg Tyr Tyr Pro His Tyr 
785                 790                 795                 800 


Phe Gly Tyr Glu Leu Thr Arg Thr Gly Gln Gly Ile Asp Gly Gly Val 
                805                 810                 815     


Ala Glu Asn Ala Leu Arg Leu Glu Lys Ser Pro Val Lys Lys Arg Glu 
            820                 825                 830         


Ile Lys Cys Lys Gln Tyr Lys Thr Leu Gly Arg Gly Gln Asn Lys Ile 
        835                 840                 845             


Val Leu Tyr Val Arg Ser Ser Tyr Tyr Gln Thr Gln Phe Leu Glu Trp 
    850                 855                 860                 


Phe Leu His Arg Pro Lys Asn Val Gln Thr Asp Val Ala Val Ser Gly 
865                 870                 875                 880 


Ser Phe Leu Ile Asp Glu Lys Lys Val Lys Thr Arg Trp Asn Tyr Asp 
                885                 890                 895     


Ala Leu Thr Val Ala Leu Glu Pro Val Ser Gly Ser Glu Arg Val Phe 
            900                 905                 910         


Val Ser Gln Pro Phe Thr Ile Phe Pro Glu Lys Ser Ala Glu Glu Glu 
        915                 920                 925             


Gly Gln Arg Tyr Leu Gly Ile Asp Ile Gly Glu Tyr Gly Ile Ala Tyr 
    930                 935                 940                 


Thr Ala Leu Glu Ile Thr Gly Asp Ser Ala Lys Ile Leu Asp Gln Asn 
945                 950                 955                 960 


Phe Ile Ser Asp Pro Gln Leu Lys Thr Leu Arg Glu Glu Val Lys Gly 
                965                 970                 975     


Leu Lys Leu Asp Gln Arg Arg Gly Thr Phe Ala Met Pro Ser Thr Lys 
            980                 985                 990         


Ile Ala Arg Ile Arg Glu Ser Leu  Val His Ser Leu Arg  Asn Arg Ile 
        995                 1000                 1005             


His His  Leu Ala Leu Lys His  Lys Ala Lys Ile Val  Tyr Glu Leu 
    1010                 1015                 1020             


Glu Val  Ser Arg Phe Glu Glu  Gly Lys Gln Lys Ile  Lys Lys Val 
    1025                 1030                 1035             


Tyr Ala  Thr Leu Lys Lys Ala  Asp Val Tyr Ser Glu  Ile Asp Ala 
    1040                 1045                 1050             


Asp Lys  Asn Leu Gln Thr Thr  Val Trp Gly Lys Leu  Ala Val Ala 
    1055                 1060                 1065             


Ser Glu  Ile Ser Ala Ser Tyr  Thr Ser Gln Phe Cys  Gly Ala Cys 
    1070                 1075                 1080             


Lys Lys  Leu Trp Arg Ala Glu  Met Gln Val Asp Glu  Thr Ile Thr 
    1085                 1090                 1095             


Thr Gln  Glu Leu Ile Gly Thr  Val Arg Val Ile Lys  Gly Gly Thr 
    1100                 1105                 1110             


Leu Ile  Asp Ala Ile Lys Asp  Phe Met Arg Pro Pro  Ile Phe Asp 
    1115                 1120                 1125             


Glu Asn  Asp Thr Pro Phe Pro  Lys Tyr Arg Asp Phe  Cys Asp Lys 
    1130                 1135                 1140             


His His  Ile Ser Lys Lys Met  Arg Gly Asn Ser Cys  Leu Phe Ile 
    1145                 1150                 1155             


Cys Pro  Phe Cys Arg Ala Asn  Ala Asp Ala Asp Ile  Gln Ala Ser 
    1160                 1165                 1170             


Gln Thr  Ile Ala Leu Leu Arg  Tyr Val Lys Glu Glu  Lys Lys Val 
    1175                 1180                 1185             


Glu Asp  Tyr Phe Glu Arg Phe  Arg Lys Leu Lys Asn  Ile Lys Val 
    1190                 1195                 1200             


Leu Gly  Gln Met Lys Lys Ile  
    1205                 1210 


<210>  209
<211>  1082
<212>  PRT
<213>  Neisseria meningitidis


<220>
<221>  MISC_FEATURE
<222>  (1)..(1082)
<223>  NCBI Reference:  WP_002235162.1; type II CRISPR RNA-guided 
       endonuclease Cas9; Nme1Cas9 polypeptide sequence

<400>  209

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  210
<211>  1082
<212>  PRT
<213>  Neisseria meningitidis


<220>
<221>  MISC_FEATURE
<222>  (1)..(1082)
<223>  NCBI Reference:  WP_002230835; type II CRISPR RNA-guided 
       endonuclease Cas9; Nme2Cas9 polypeptide sequence

<400>  210

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asn Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Ser Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Val Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Val 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Cys Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp His Ile Leu Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Arg Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Lys Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Ala His Lys Asp Thr Leu Arg Ser 
        835                 840                 845             


Ala Lys Arg Phe Val Lys His Asn Glu Lys Ile Ser Val Lys Arg Val 
    850                 855                 860                 


Trp Leu Thr Glu Ile Lys Leu Ala Asp Leu Glu Asn Met Val Asn Tyr 
865                 870                 875                 880 


Lys Asn Gly Arg Glu Ile Glu Leu Tyr Glu Ala Leu Lys Ala Arg Leu 
                885                 890                 895     


Glu Ala Tyr Gly Gly Asn Ala Lys Gln Ala Phe Asp Pro Lys Asp Asn 
            900                 905                 910         


Pro Phe Tyr Lys Lys Gly Gly Gln Leu Val Lys Ala Val Arg Val Glu 
        915                 920                 925             


Lys Thr Gln Glu Ser Gly Val Leu Leu Asn Lys Lys Asn Ala Tyr Thr 
    930                 935                 940                 


Ile Ala Asp Asn Gly Asp Met Val Arg Val Asp Val Phe Cys Lys Val 
945                 950                 955                 960 


Asp Lys Lys Gly Lys Asn Gln Tyr Phe Ile Val Pro Ile Tyr Ala Trp 
                965                 970                 975     


Gln Val Ala Glu Asn Ile Leu Pro Asp Ile Asp Cys Lys Gly Tyr Arg 
            980                 985                 990         


Ile Asp Asp Ser Tyr Thr Phe Cys  Phe Ser Leu His Lys  Tyr Asp Leu 
        995                 1000                 1005             


Ile Ala  Phe Gln Lys Asp Glu  Lys Ser Lys Val Glu  Phe Ala Tyr 
    1010                 1015                 1020             


Tyr Ile  Asn Cys Asp Ser Ser  Asn Gly Arg Phe Tyr  Leu Ala Trp 
    1025                 1030                 1035             


His Asp  Lys Gly Ser Lys Glu  Gln Gln Phe Arg Ile  Ser Thr Gln 
    1040                 1045                 1050             


Asn Leu  Val Leu Ile Gln Lys  Tyr Gln Val Asn Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  211
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  wild type Cpf1 polypeptide sequence

<400>  211

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  212
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 D917A polypeptide sequence

<400>  212

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Ala Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  213
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 E1006A polypeptide sequence

<400>  213

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Ala Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  214
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 D1255A polypeptide sequence

<400>  214

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Ala  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  215
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 D917A/E1006A polypeptide sequence

<400>  215

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Ala Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Ala Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  216
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 D917A/D1255A polypeptide sequence

<400>  216

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Ala Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Ala  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  217
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 E1006A/D1255A polypeptide sequence

<400>  217

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Ala Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Ala  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  218
<211>  1300
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Cpf1 D917A/E1006A/D1255A polypeptide sequence

<400>  218

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Ala Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Ala Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Ala  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  219
<211>  1052
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1052)
<223>  Exemplary SaCas9 polypeptide sequence

<400>  219

Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile Gln 
    50                  55                  60                  


Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His Ser 
65                  70                  75                  80  


Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu Ser 
                85                  90                  95      


Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu Ala 
            100                 105                 110         


Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr Gly 
        115                 120                 125             


Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala Leu 
    130                 135                 140                 


Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys Asp 
145                 150                 155                 160 


Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr Val 
                165                 170                 175     


Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln Leu 
            180                 185                 190         


Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg Arg 
        195                 200                 205             


Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys Asp 
    210                 215                 220                 


Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro 
225                 230                 235                 240 


Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr Asn 
                245                 250                 255     


Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn Glu 
            260                 265                 270         


Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys 
        275                 280                 285             


Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu Val 
    290                 295                 300                 


Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys Pro 
305                 310                 315                 320 


Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr Ala 
                325                 330                 335     


Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala Lys 
            340                 345                 350         


Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu Thr 
        355                 360                 365             


Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser Asn 
    370                 375                 380                 


Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile Asn 
385                 390                 395                 400 


Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala Ile 
                405                 410                 415     


Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln Gln 
            420                 425                 430         


Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro Val 
        435                 440                 445             


Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile Ile 
    450                 455                 460                 


Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg Glu 
465                 470                 475                 480 


Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys Arg 
                485                 490                 495     


Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr Gly 
            500                 505                 510         


Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp Met 
        515                 520                 525             


Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu Asp 
    530                 535                 540                 


Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro Arg 
545                 550                 555                 560 


Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys Gln 
                565                 570                 575     


Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu Ser 
            580                 585                 590         


Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile Leu 
        595                 600                 605             


Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu Tyr 
    610                 615                 620                 


Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp Phe 
625                 630                 635                 640 


Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu Met 
                645                 650                 655     


Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys Val 
            660                 665                 670         


Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp Lys 
        675                 680                 685             


Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp Ala 
    690                 695                 700                 


Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys Leu 
705                 710                 715                 720 


Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys Gln 
                725                 730                 735     


Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu Ile 
            740                 745                 750         


Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp Tyr 
        755                 760                 765             


Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile Asn 
    770                 775                 780                 


Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu Ile 
785                 790                 795                 800 


Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu Lys 
                805                 810                 815     


Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His Asp 
            820                 825                 830         


Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly Asp 
        835                 840                 845             


Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile Lys 
865                 870                 875                 880 


Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp Tyr 
                885                 890                 895     


Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val Lys 
        915                 920                 925             


Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser Lys 
    930                 935                 940                 


Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala Glu 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly Glu 
                965                 970                 975     


Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile Glu 
            980                 985                 990         


Val Asn Met Ile Asp Ile Thr Tyr  Arg Glu Tyr Leu Glu  Asn Met Asn 
        995                 1000                 1005             


Asp Lys  Arg Pro Pro Arg Ile  Ile Lys Thr Ile Ala  Ser Lys Thr 
    1010                 1015                 1020             


Gln Ser  Ile Lys Lys Tyr Ser  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Glu Val  Lys Ser Lys Lys His  Pro Gln Ile Ile Lys  Lys Gly 
    1040                 1045                 1050         


<210>  220
<211>  1052
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1052)
<223>  SaCas9n polypeptide sequence

<400>  220

Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile Gln 
    50                  55                  60                  


Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His Ser 
65                  70                  75                  80  


Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu Ser 
                85                  90                  95      


Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu Ala 
            100                 105                 110         


Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr Gly 
        115                 120                 125             


Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala Leu 
    130                 135                 140                 


Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys Asp 
145                 150                 155                 160 


Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr Val 
                165                 170                 175     


Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln Leu 
            180                 185                 190         


Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg Arg 
        195                 200                 205             


Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys Asp 
    210                 215                 220                 


Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro 
225                 230                 235                 240 


Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr Asn 
                245                 250                 255     


Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn Glu 
            260                 265                 270         


Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys 
        275                 280                 285             


Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu Val 
    290                 295                 300                 


Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys Pro 
305                 310                 315                 320 


Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr Ala 
                325                 330                 335     


Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala Lys 
            340                 345                 350         


Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu Thr 
        355                 360                 365             


Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser Asn 
    370                 375                 380                 


Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile Asn 
385                 390                 395                 400 


Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala Ile 
                405                 410                 415     


Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln Gln 
            420                 425                 430         


Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro Val 
        435                 440                 445             


Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile Ile 
    450                 455                 460                 


Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg Glu 
465                 470                 475                 480 


Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys Arg 
                485                 490                 495     


Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr Gly 
            500                 505                 510         


Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp Met 
        515                 520                 525             


Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu Asp 
    530                 535                 540                 


Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro Arg 
545                 550                 555                 560 


Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys Gln 
                565                 570                 575     


Glu Glu Ala Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu Ser 
            580                 585                 590         


Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile Leu 
        595                 600                 605             


Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu Tyr 
    610                 615                 620                 


Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp Phe 
625                 630                 635                 640 


Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu Met 
                645                 650                 655     


Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys Val 
            660                 665                 670         


Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp Lys 
        675                 680                 685             


Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp Ala 
    690                 695                 700                 


Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys Leu 
705                 710                 715                 720 


Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys Gln 
                725                 730                 735     


Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu Ile 
            740                 745                 750         


Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp Tyr 
        755                 760                 765             


Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile Asn 
    770                 775                 780                 


Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu Ile 
785                 790                 795                 800 


Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu Lys 
                805                 810                 815     


Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His Asp 
            820                 825                 830         


Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly Asp 
        835                 840                 845             


Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile Lys 
865                 870                 875                 880 


Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp Tyr 
                885                 890                 895     


Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val Lys 
        915                 920                 925             


Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser Lys 
    930                 935                 940                 


Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala Glu 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly Glu 
                965                 970                 975     


Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile Glu 
            980                 985                 990         


Val Asn Met Ile Asp Ile Thr Tyr  Arg Glu Tyr Leu Glu  Asn Met Asn 
        995                 1000                 1005             


Asp Lys  Arg Pro Pro Arg Ile  Ile Lys Thr Ile Ala  Ser Lys Thr 
    1010                 1015                 1020             


Gln Ser  Ile Lys Lys Tyr Ser  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Glu Val  Lys Ser Lys Lys His  Pro Gln Ile Ile Lys  Lys Gly 
    1040                 1045                 1050         


<210>  221
<211>  1052
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1052)
<223>  SaKKH Cas9 polypeptide sequence

<400>  221

Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly 
1               5                   10                  15      


Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly Val 
            20                  25                  30          


Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg Ser 
        35                  40                  45              


Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile Gln 
    50                  55                  60                  


Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His Ser 
65                  70                  75                  80  


Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu Ser 
                85                  90                  95      


Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu Ala 
            100                 105                 110         


Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr Gly 
        115                 120                 125             


Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala Leu 
    130                 135                 140                 


Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys Asp 
145                 150                 155                 160 


Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr Val 
                165                 170                 175     


Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln Leu 
            180                 185                 190         


Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg Arg 
        195                 200                 205             


Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys Asp 
    210                 215                 220                 


Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro 
225                 230                 235                 240 


Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr Asn 
                245                 250                 255     


Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn Glu 
            260                 265                 270         


Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys 
        275                 280                 285             


Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu Val 
    290                 295                 300                 


Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys Pro 
305                 310                 315                 320 


Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr Ala 
                325                 330                 335     


Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala Lys 
            340                 345                 350         


Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu Thr 
        355                 360                 365             


Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser Asn 
    370                 375                 380                 


Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile Asn 
385                 390                 395                 400 


Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala Ile 
                405                 410                 415     


Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln Gln 
            420                 425                 430         


Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro Val 
        435                 440                 445             


Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile Ile 
    450                 455                 460                 


Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg Glu 
465                 470                 475                 480 


Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys Arg 
                485                 490                 495     


Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr Gly 
            500                 505                 510         


Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp Met 
        515                 520                 525             


Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu Asp 
    530                 535                 540                 


Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro Arg 
545                 550                 555                 560 


Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys Gln 
                565                 570                 575     


Glu Glu Ala Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu Ser 
            580                 585                 590         


Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile Leu 
        595                 600                 605             


Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu Tyr 
    610                 615                 620                 


Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp Phe 
625                 630                 635                 640 


Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu Met 
                645                 650                 655     


Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys Val 
            660                 665                 670         


Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp Lys 
        675                 680                 685             


Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp Ala 
    690                 695                 700                 


Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys Leu 
705                 710                 715                 720 


Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys Gln 
                725                 730                 735     


Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu Ile 
            740                 745                 750         


Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp Tyr 
        755                 760                 765             


Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Lys Leu Ile Asn 
    770                 775                 780                 


Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu Ile 
785                 790                 795                 800 


Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu Lys 
                805                 810                 815     


Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His Asp 
            820                 825                 830         


Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly Asp 
        835                 840                 845             


Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile Lys 
865                 870                 875                 880 


Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp Tyr 
                885                 890                 895     


Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val Lys 
        915                 920                 925             


Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser Lys 
    930                 935                 940                 


Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala Glu 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Ile Asn Gly Glu 
                965                 970                 975     


Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile Glu 
            980                 985                 990         


Val Asn Met Ile Asp Ile Thr Tyr  Arg Glu Tyr Leu Glu  Asn Met Asn 
        995                 1000                 1005             


Asp Lys  Arg Pro Pro His Ile  Ile Lys Thr Ile Ala  Ser Lys Thr 
    1010                 1015                 1020             


Gln Ser  Ile Lys Lys Tyr Ser  Thr Asp Ile Leu Gly  Asn Leu Tyr 
    1025                 1030                 1035             


Glu Val  Lys Ser Lys Lys His  Pro Gln Ile Ile Lys  Lys Gly 
    1040                 1045                 1050         


<210>  222
<211>  707
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-1 polypeptide sequence

<400>  222

Met Ala Asp Thr Pro Thr Leu Phe Thr Gln Phe Leu Arg His His Leu 
1               5                   10                  15      


Pro Gly Gln Arg Phe Arg Lys Asp Ile Leu Lys Gln Ala Gly Arg Ile 
            20                  25                  30          


Leu Ala Asn Lys Gly Glu Asp Ala Thr Ile Ala Phe Leu Arg Gly Lys 
        35                  40                  45              


Ser Glu Glu Ser Pro Pro Asp Phe Gln Pro Pro Val Lys Cys Pro Ile 
    50                  55                  60                  


Ile Ala Cys Ser Arg Pro Leu Thr Glu Trp Pro Ile Tyr Gln Ala Ser 
65                  70                  75                  80  


Val Ala Ile Gln Gly Tyr Val Tyr Gly Gln Ser Leu Ala Glu Phe Glu 
                85                  90                  95      


Ala Ser Asp Pro Gly Cys Ser Lys Asp Gly Leu Leu Gly Trp Phe Asp 
            100                 105                 110         


Lys Thr Gly Val Cys Thr Asp Tyr Phe Ser Val Gln Gly Leu Asn Leu 
        115                 120                 125             


Ile Phe Gln Asn Ala Arg Lys Arg Tyr Ile Gly Val Gln Thr Lys Val 
    130                 135                 140                 


Thr Asn Arg Asn Glu Lys Arg His Lys Lys Leu Lys Arg Ile Asn Ala 
145                 150                 155                 160 


Lys Arg Ile Ala Glu Gly Leu Pro Glu Leu Thr Ser Asp Glu Pro Glu 
                165                 170                 175     


Ser Ala Leu Asp Glu Thr Gly His Leu Ile Asp Pro Pro Gly Leu Asn 
            180                 185                 190         


Thr Asn Ile Tyr Cys Tyr Gln Gln Val Ser Pro Lys Pro Leu Ala Leu 
        195                 200                 205             


Ser Glu Val Asn Gln Leu Pro Thr Ala Tyr Ala Gly Tyr Ser Thr Ser 
    210                 215                 220                 


Gly Asp Asp Pro Ile Gln Pro Met Val Thr Lys Asp Arg Leu Ser Ile 
225                 230                 235                 240 


Ser Lys Gly Gln Pro Gly Tyr Ile Pro Glu His Gln Arg Ala Leu Leu 
                245                 250                 255     


Ser Gln Lys Lys His Arg Arg Met Arg Gly Tyr Gly Leu Lys Ala Arg 
            260                 265                 270         


Ala Leu Leu Val Ile Val Arg Ile Gln Asp Asp Trp Ala Val Ile Asp 
        275                 280                 285             


Leu Arg Ser Leu Leu Arg Asn Ala Tyr Trp Arg Arg Ile Val Gln Thr 
    290                 295                 300                 


Lys Glu Pro Ser Thr Ile Thr Lys Leu Leu Lys Leu Val Thr Gly Asp 
305                 310                 315                 320 


Pro Val Leu Asp Ala Thr Arg Met Val Ala Thr Phe Thr Tyr Lys Pro 
                325                 330                 335     


Gly Ile Val Gln Val Arg Ser Ala Lys Cys Leu Lys Asn Lys Gln Gly 
            340                 345                 350         


Ser Lys Leu Phe Ser Glu Arg Tyr Leu Asn Glu Thr Val Ser Val Thr 
        355                 360                 365             


Ser Ile Asp Leu Gly Ser Asn Asn Leu Val Ala Val Ala Thr Tyr Arg 
    370                 375                 380                 


Leu Val Asn Gly Asn Thr Pro Glu Leu Leu Gln Arg Phe Thr Leu Pro 
385                 390                 395                 400 


Ser His Leu Val Lys Asp Phe Glu Arg Tyr Lys Gln Ala His Asp Thr 
                405                 410                 415     


Leu Glu Asp Ser Ile Gln Lys Thr Ala Val Ala Ser Leu Pro Gln Gly 
            420                 425                 430         


Gln Gln Thr Glu Ile Arg Met Trp Ser Met Tyr Gly Phe Arg Glu Ala 
        435                 440                 445             


Gln Glu Arg Val Cys Gln Glu Leu Gly Leu Ala Asp Gly Ser Ile Pro 
    450                 455                 460                 


Trp Asn Val Met Thr Ala Thr Ser Thr Ile Leu Thr Asp Leu Phe Leu 
465                 470                 475                 480 


Ala Arg Gly Gly Asp Pro Lys Lys Cys Met Phe Thr Ser Glu Pro Lys 
                485                 490                 495     


Lys Lys Lys Asn Ser Lys Gln Val Leu Tyr Lys Ile Arg Asp Arg Ala 
            500                 505                 510         


Trp Ala Lys Met Tyr Arg Thr Leu Leu Ser Lys Glu Thr Arg Glu Ala 
        515                 520                 525             


Trp Asn Lys Ala Leu Trp Gly Leu Lys Arg Gly Ser Pro Asp Tyr Ala 
    530                 535                 540                 


Arg Leu Ser Lys Arg Lys Glu Glu Leu Ala Arg Arg Cys Val Asn Tyr 
545                 550                 555                 560 


Thr Ile Ser Thr Ala Glu Lys Arg Ala Gln Cys Gly Arg Thr Ile Val 
                565                 570                 575     


Ala Leu Glu Asp Leu Asn Ile Gly Phe Phe His Gly Arg Gly Lys Gln 
            580                 585                 590         


Glu Pro Gly Trp Val Gly Leu Phe Thr Arg Lys Lys Glu Asn Arg Trp 
        595                 600                 605             


Leu Met Gln Ala Leu His Lys Ala Phe Leu Glu Leu Ala His His Arg 
    610                 615                 620                 


Gly Tyr His Val Ile Glu Val Asn Pro Ala Tyr Thr Ser Gln Thr Cys 
625                 630                 635                 640 


Pro Val Cys Arg His Cys Asp Pro Asp Asn Arg Asp Gln His Asn Arg 
                645                 650                 655     


Glu Ala Phe His Cys Ile Gly Cys Gly Phe Arg Gly Asn Ala Asp Leu 
            660                 665                 670         


Asp Val Ala Thr His Asn Ile Ala Met Val Ala Ile Thr Gly Glu Ser 
        675                 680                 685             


Leu Lys Arg Ala Arg Gly Ser Val Ala Ser Lys Thr Pro Gln Pro Leu 
    690                 695                 700                 


Ala Ala Glu 
705         


<210>  223
<211>  757
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-2 polypeptide sequence

<400>  223

Met Pro Lys Pro Ala Val Glu Ser Glu Phe Ser Lys Val Leu Lys Lys 
1               5                   10                  15      


His Phe Pro Gly Glu Arg Phe Arg Ser Ser Tyr Met Lys Arg Gly Gly 
            20                  25                  30          


Lys Ile Leu Ala Ala Gln Gly Glu Glu Ala Val Val Ala Tyr Leu Gln 
        35                  40                  45              


Gly Lys Ser Glu Glu Glu Pro Pro Asn Phe Gln Pro Pro Ala Lys Cys 
    50                  55                  60                  


His Val Val Thr Lys Ser Arg Asp Phe Ala Glu Trp Pro Ile Met Lys 
65                  70                  75                  80  


Ala Ser Glu Ala Ile Gln Arg Tyr Ile Tyr Ala Leu Ser Thr Thr Glu 
                85                  90                  95      


Arg Ala Ala Cys Lys Pro Gly Lys Ser Ser Glu Ser His Ala Ala Trp 
            100                 105                 110         


Phe Ala Ala Thr Gly Val Ser Asn His Gly Tyr Ser His Val Gln Gly 
        115                 120                 125             


Leu Asn Leu Ile Phe Asp His Thr Leu Gly Arg Tyr Asp Gly Val Leu 
    130                 135                 140                 


Lys Lys Val Gln Leu Arg Asn Glu Lys Ala Arg Ala Arg Leu Glu Ser 
145                 150                 155                 160 


Ile Asn Ala Ser Arg Ala Asp Glu Gly Leu Pro Glu Ile Lys Ala Glu 
                165                 170                 175     


Glu Glu Glu Val Ala Thr Asn Glu Thr Gly His Leu Leu Gln Pro Pro 
            180                 185                 190         


Gly Ile Asn Pro Ser Phe Tyr Val Tyr Gln Thr Ile Ser Pro Gln Ala 
        195                 200                 205             


Tyr Arg Pro Arg Asp Glu Ile Val Leu Pro Pro Glu Tyr Ala Gly Tyr 
    210                 215                 220                 


Val Arg Asp Pro Asn Ala Pro Ile Pro Leu Gly Val Val Arg Asn Arg 
225                 230                 235                 240 


Cys Asp Ile Gln Lys Gly Cys Pro Gly Tyr Ile Pro Glu Trp Gln Arg 
                245                 250                 255     


Glu Ala Gly Thr Ala Ile Ser Pro Lys Thr Gly Lys Ala Val Thr Val 
            260                 265                 270         


Pro Gly Leu Ser Pro Lys Lys Asn Lys Arg Met Arg Arg Tyr Trp Arg 
        275                 280                 285             


Ser Glu Lys Glu Lys Ala Gln Asp Ala Leu Leu Val Thr Val Arg Ile 
    290                 295                 300                 


Gly Thr Asp Trp Val Val Ile Asp Val Arg Gly Leu Leu Arg Asn Ala 
305                 310                 315                 320 


Arg Trp Arg Thr Ile Ala Pro Lys Asp Ile Ser Leu Asn Ala Leu Leu 
                325                 330                 335     


Asp Leu Phe Thr Gly Asp Pro Val Ile Asp Val Arg Arg Asn Ile Val 
            340                 345                 350         


Thr Phe Thr Tyr Thr Leu Asp Ala Cys Gly Thr Tyr Ala Arg Lys Trp 
        355                 360                 365             


Thr Leu Lys Gly Lys Gln Thr Lys Ala Thr Leu Asp Lys Leu Thr Ala 
    370                 375                 380                 


Thr Gln Thr Val Ala Leu Val Ala Ile Asp Leu Gly Gln Thr Asn Pro 
385                 390                 395                 400 


Ile Ser Ala Gly Ile Ser Arg Val Thr Gln Glu Asn Gly Ala Leu Gln 
                405                 410                 415     


Cys Glu Pro Leu Asp Arg Phe Thr Leu Pro Asp Asp Leu Leu Lys Asp 
            420                 425                 430         


Ile Ser Ala Tyr Arg Ile Ala Trp Asp Arg Asn Glu Glu Glu Leu Arg 
        435                 440                 445             


Ala Arg Ser Val Glu Ala Leu Pro Glu Ala Gln Gln Ala Glu Val Arg 
    450                 455                 460                 


Ala Leu Asp Gly Val Ser Lys Glu Thr Ala Arg Thr Gln Leu Cys Ala 
465                 470                 475                 480 


Asp Phe Gly Leu Asp Pro Lys Arg Leu Pro Trp Asp Lys Met Ser Ser 
                485                 490                 495     


Asn Thr Thr Phe Ile Ser Glu Ala Leu Leu Ser Asn Ser Val Ser Arg 
            500                 505                 510         


Asp Gln Val Phe Phe Thr Pro Ala Pro Lys Lys Gly Ala Lys Lys Lys 
        515                 520                 525             


Ala Pro Val Glu Val Met Arg Lys Asp Arg Thr Trp Ala Arg Ala Tyr 
    530                 535                 540                 


Lys Pro Arg Leu Ser Val Glu Ala Gln Lys Leu Lys Asn Glu Ala Leu 
545                 550                 555                 560 


Trp Ala Leu Lys Arg Thr Ser Pro Glu Tyr Leu Lys Leu Ser Arg Arg 
                565                 570                 575     


Lys Glu Glu Leu Cys Arg Arg Ser Ile Asn Tyr Val Ile Glu Lys Thr 
            580                 585                 590         


Arg Arg Arg Thr Gln Cys Gln Ile Val Ile Pro Val Ile Glu Asp Leu 
        595                 600                 605             


Asn Val Arg Phe Phe His Gly Ser Gly Lys Arg Leu Pro Gly Trp Asp 
    610                 615                 620                 


Asn Phe Phe Thr Ala Lys Lys Glu Asn Arg Trp Phe Ile Gln Gly Leu 
625                 630                 635                 640 


His Lys Ala Phe Ser Asp Leu Arg Thr His Arg Ser Phe Tyr Val Phe 
                645                 650                 655     


Glu Val Arg Pro Glu Arg Thr Ser Ile Thr Cys Pro Lys Cys Gly His 
            660                 665                 670         


Cys Glu Val Gly Asn Arg Asp Gly Glu Ala Phe Gln Cys Leu Ser Cys 
        675                 680                 685             


Gly Lys Thr Cys Asn Ala Asp Leu Asp Val Ala Thr His Asn Leu Thr 
    690                 695                 700                 


Gln Val Ala Leu Thr Gly Lys Thr Met Pro Lys Arg Glu Glu Pro Arg 
705                 710                 715                 720 


Asp Ala Gln Gly Thr Ala Pro Ala Arg Lys Thr Lys Lys Ala Ser Lys 
                725                 730                 735     


Ser Lys Ala Pro Pro Ala Glu Arg Glu Asp Gln Thr Pro Ala Gln Glu 
            740                 745                 750         


Pro Ser Gln Thr Ser 
        755         


<210>  224
<211>  766
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-3 polypeptide sequence

<400>  224

Met Glu Lys Glu Ile Thr Glu Leu Thr Lys Ile Arg Arg Glu Phe Pro 
1               5                   10                  15      


Asn Lys Lys Phe Ser Ser Thr Asp Met Lys Lys Ala Gly Lys Leu Leu 
            20                  25                  30          


Lys Ala Glu Gly Pro Asp Ala Val Arg Asp Phe Leu Asn Ser Cys Gln 
        35                  40                  45              


Glu Ile Ile Gly Asp Phe Lys Pro Pro Val Lys Thr Asn Ile Val Ser 
    50                  55                  60                  


Ile Ser Arg Pro Phe Glu Glu Trp Pro Val Ser Met Val Gly Arg Ala 
65                  70                  75                  80  


Ile Gln Glu Tyr Tyr Phe Ser Leu Thr Lys Glu Glu Leu Glu Ser Val 
                85                  90                  95      


His Pro Gly Thr Ser Ser Glu Asp His Lys Ser Phe Phe Asn Ile Thr 
            100                 105                 110         


Gly Leu Ser Asn Tyr Asn Tyr Thr Ser Val Gln Gly Leu Asn Leu Ile 
        115                 120                 125             


Phe Lys Asn Ala Lys Ala Ile Tyr Asp Gly Thr Leu Val Lys Ala Asn 
    130                 135                 140                 


Asn Lys Asn Lys Lys Leu Glu Lys Lys Phe Asn Glu Ile Asn His Lys 
145                 150                 155                 160 


Arg Ser Leu Glu Gly Leu Pro Ile Ile Thr Pro Asp Phe Glu Glu Pro 
                165                 170                 175     


Phe Asp Glu Asn Gly His Leu Asn Asn Pro Pro Gly Ile Asn Arg Asn 
            180                 185                 190         


Ile Tyr Gly Tyr Gln Gly Cys Ala Ala Lys Val Phe Val Pro Ser Lys 
        195                 200                 205             


His Lys Met Val Ser Leu Pro Lys Glu Tyr Glu Gly Tyr Asn Arg Asp 
    210                 215                 220                 


Pro Asn Leu Ser Leu Ala Gly Phe Arg Asn Arg Leu Glu Ile Pro Glu 
225                 230                 235                 240 


Gly Glu Pro Gly His Val Pro Trp Phe Gln Arg Met Asp Ile Pro Glu 
                245                 250                 255     


Gly Gln Ile Gly His Val Asn Lys Ile Gln Arg Phe Asn Phe Val His 
            260                 265                 270         


Gly Lys Asn Ser Gly Lys Val Lys Phe Ser Asp Lys Thr Gly Arg Val 
        275                 280                 285             


Lys Arg Tyr His His Ser Lys Tyr Lys Asp Ala Thr Lys Pro Tyr Lys 
    290                 295                 300                 


Phe Leu Glu Glu Ser Lys Lys Val Ser Ala Leu Asp Ser Ile Leu Ala 
305                 310                 315                 320 


Ile Ile Thr Ile Gly Asp Asp Trp Val Val Phe Asp Ile Arg Gly Leu 
                325                 330                 335     


Tyr Arg Asn Val Phe Tyr Arg Glu Leu Ala Gln Lys Gly Leu Thr Ala 
            340                 345                 350         


Val Gln Leu Leu Asp Leu Phe Thr Gly Asp Pro Val Ile Asp Pro Lys 
        355                 360                 365             


Lys Gly Val Val Thr Phe Ser Tyr Lys Glu Gly Val Val Pro Val Phe 
    370                 375                 380                 


Ser Gln Lys Ile Val Pro Arg Phe Lys Ser Arg Asp Thr Leu Glu Lys 
385                 390                 395                 400 


Leu Thr Ser Gln Gly Pro Val Ala Leu Leu Ser Val Asp Leu Gly Gln 
                405                 410                 415     


Asn Glu Pro Val Ala Ala Arg Val Cys Ser Leu Lys Asn Ile Asn Asp 
            420                 425                 430         


Lys Ile Thr Leu Asp Asn Ser Cys Arg Ile Ser Phe Leu Asp Asp Tyr 
        435                 440                 445             


Lys Lys Gln Ile Lys Asp Tyr Arg Asp Ser Leu Asp Glu Leu Glu Ile 
    450                 455                 460                 


Lys Ile Arg Leu Glu Ala Ile Asn Ser Leu Glu Thr Asn Gln Gln Val 
465                 470                 475                 480 


Glu Ile Arg Asp Leu Asp Val Phe Ser Ala Asp Arg Ala Lys Ala Asn 
                485                 490                 495     


Thr Val Asp Met Phe Asp Ile Asp Pro Asn Leu Ile Ser Trp Asp Ser 
            500                 505                 510         


Met Ser Asp Ala Arg Val Ser Thr Gln Ile Ser Asp Leu Tyr Leu Lys 
        515                 520                 525             


Asn Gly Gly Asp Glu Ser Arg Val Tyr Phe Glu Ile Asn Asn Lys Arg 
    530                 535                 540                 


Ile Lys Arg Ser Asp Tyr Asn Ile Ser Gln Leu Val Arg Pro Lys Leu 
545                 550                 555                 560 


Ser Asp Ser Thr Arg Lys Asn Leu Asn Asp Ser Ile Trp Lys Leu Lys 
                565                 570                 575     


Arg Thr Ser Glu Glu Tyr Leu Lys Leu Ser Lys Arg Lys Leu Glu Leu 
            580                 585                 590         


Ser Arg Ala Val Val Asn Tyr Thr Ile Arg Gln Ser Lys Leu Leu Ser 
        595                 600                 605             


Gly Ile Asn Asp Ile Val Ile Ile Leu Glu Asp Leu Asp Val Lys Lys 
    610                 615                 620                 


Lys Phe Asn Gly Arg Gly Ile Arg Asp Ile Gly Trp Asp Asn Phe Phe 
625                 630                 635                 640 


Ser Ser Arg Lys Glu Asn Arg Trp Phe Ile Pro Ala Phe His Lys Ala 
                645                 650                 655     


Phe Ser Glu Leu Ser Ser Asn Arg Gly Leu Cys Val Ile Glu Val Asn 
            660                 665                 670         


Pro Ala Trp Thr Ser Ala Thr Cys Pro Asp Cys Gly Phe Cys Ser Lys 
        675                 680                 685             


Glu Asn Arg Asp Gly Ile Asn Phe Thr Cys Arg Lys Cys Gly Val Ser 
    690                 695                 700                 


Tyr His Ala Asp Ile Asp Val Ala Thr Leu Asn Ile Ala Arg Val Ala 
705                 710                 715                 720 


Val Leu Gly Lys Pro Met Ser Gly Pro Ala Asp Arg Glu Arg Leu Gly 
                725                 730                 735     


Asp Thr Lys Lys Pro Arg Val Ala Arg Ser Arg Lys Thr Met Lys Arg 
            740                 745                 750         


Lys Asp Ile Ser Asn Ser Thr Val Glu Ala Met Val Thr Ala 
        755                 760                 765     


<210>  225
<211>  765
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-4 polypeptide sequence

<400>  225

Met Tyr Ser Leu Glu Met Ala Asp Leu Lys Ser Glu Pro Ser Leu Leu 
1               5                   10                  15      


Ala Lys Leu Leu Arg Asp Arg Phe Pro Gly Lys Tyr Trp Leu Pro Lys 
            20                  25                  30          


Tyr Trp Lys Leu Ala Glu Lys Lys Arg Leu Thr Gly Gly Glu Glu Ala 
        35                  40                  45              


Ala Cys Glu Tyr Met Ala Asp Lys Gln Leu Asp Ser Pro Pro Pro Asn 
    50                  55                  60                  


Phe Arg Pro Pro Ala Arg Cys Val Ile Leu Ala Lys Ser Arg Pro Phe 
65                  70                  75                  80  


Glu Asp Trp Pro Val His Arg Val Ala Ser Lys Ala Gln Ser Phe Val 
                85                  90                  95      


Ile Gly Leu Ser Glu Gln Gly Phe Ala Ala Leu Arg Ala Ala Pro Pro 
            100                 105                 110         


Ser Thr Ala Asp Ala Arg Arg Asp Trp Leu Arg Ser His Gly Ala Ser 
        115                 120                 125             


Glu Asp Asp Leu Met Ala Leu Glu Ala Gln Leu Leu Glu Thr Ile Met 
    130                 135                 140                 


Gly Asn Ala Ile Ser Leu His Gly Gly Val Leu Lys Lys Ile Asp Asn 
145                 150                 155                 160 


Ala Asn Val Lys Ala Ala Lys Arg Leu Ser Gly Arg Asn Glu Ala Arg 
                165                 170                 175     


Leu Asn Lys Gly Leu Gln Glu Leu Pro Pro Glu Gln Glu Gly Ser Ala 
            180                 185                 190         


Tyr Gly Ala Asp Gly Leu Leu Val Asn Pro Pro Gly Leu Asn Leu Asn 
        195                 200                 205             


Ile Tyr Cys Arg Lys Ser Cys Cys Pro Lys Pro Val Lys Asn Thr Ala 
    210                 215                 220                 


Arg Phe Val Gly His Tyr Pro Gly Tyr Leu Arg Asp Ser Asp Ser Ile 
225                 230                 235                 240 


Leu Ile Ser Gly Thr Met Asp Arg Leu Thr Ile Ile Glu Gly Met Pro 
                245                 250                 255     


Gly His Ile Pro Ala Trp Gln Arg Glu Gln Gly Leu Val Lys Pro Gly 
            260                 265                 270         


Gly Arg Arg Arg Arg Leu Ser Gly Ser Glu Ser Asn Met Arg Gln Lys 
        275                 280                 285             


Val Asp Pro Ser Thr Gly Pro Arg Arg Ser Thr Arg Ser Gly Thr Val 
    290                 295                 300                 


Asn Arg Ser Asn Gln Arg Thr Gly Arg Asn Gly Asp Pro Leu Leu Val 
305                 310                 315                 320 


Glu Ile Arg Met Lys Glu Asp Trp Val Leu Leu Asp Ala Arg Gly Leu 
                325                 330                 335     


Leu Arg Asn Leu Arg Trp Arg Glu Ser Lys Arg Gly Leu Ser Cys Asp 
            340                 345                 350         


His Glu Asp Leu Ser Leu Ser Gly Leu Leu Ala Leu Phe Ser Gly Asp 
        355                 360                 365             


Pro Val Ile Asp Pro Val Arg Asn Glu Val Val Phe Leu Tyr Gly Glu 
    370                 375                 380                 


Gly Ile Ile Pro Val Arg Ser Thr Lys Pro Val Gly Thr Arg Gln Ser 
385                 390                 395                 400 


Lys Lys Leu Leu Glu Arg Gln Ala Ser Met Gly Pro Leu Thr Leu Ile 
                405                 410                 415     


Ser Cys Asp Leu Gly Gln Thr Asn Leu Ile Ala Gly Arg Ala Ser Ala 
            420                 425                 430         


Ile Ser Leu Thr His Gly Ser Leu Gly Val Arg Ser Ser Val Arg Ile 
        435                 440                 445             


Glu Leu Asp Pro Glu Ile Ile Lys Ser Phe Glu Arg Leu Arg Lys Asp 
    450                 455                 460                 


Ala Asp Arg Leu Glu Thr Glu Ile Leu Thr Ala Ala Lys Glu Thr Leu 
465                 470                 475                 480 


Ser Asp Glu Gln Arg Gly Glu Val Asn Ser His Glu Lys Asp Ser Pro 
                485                 490                 495     


Gln Thr Ala Lys Ala Ser Leu Cys Arg Glu Leu Gly Leu His Pro Pro 
            500                 505                 510         


Ser Leu Pro Trp Gly Gln Met Gly Pro Ser Thr Thr Phe Ile Ala Asp 
        515                 520                 525             


Met Leu Ile Ser His Gly Arg Asp Asp Asp Ala Phe Leu Ser His Gly 
    530                 535                 540                 


Glu Phe Pro Thr Leu Glu Lys Arg Lys Lys Phe Asp Lys Arg Phe Cys 
545                 550                 555                 560 


Leu Glu Ser Arg Pro Leu Leu Ser Ser Glu Thr Arg Lys Ala Leu Asn 
                565                 570                 575     


Glu Ser Leu Trp Glu Val Lys Arg Thr Ser Ser Glu Tyr Ala Arg Leu 
            580                 585                 590         


Ser Gln Arg Lys Lys Glu Met Ala Arg Arg Ala Val Asn Phe Val Val 
        595                 600                 605             


Glu Ile Ser Arg Arg Lys Thr Gly Leu Ser Asn Val Ile Val Asn Ile 
    610                 615                 620                 


Glu Asp Leu Asn Val Arg Ile Phe His Gly Gly Gly Lys Gln Ala Pro 
625                 630                 635                 640 


Gly Trp Asp Gly Phe Phe Arg Pro Lys Ser Glu Asn Arg Trp Phe Ile 
                645                 650                 655     


Gln Ala Ile His Lys Ala Phe Ser Asp Leu Ala Ala His His Gly Ile 
            660                 665                 670         


Pro Val Ile Glu Ser Asp Pro Gln Arg Thr Ser Met Thr Cys Pro Glu 
        675                 680                 685             


Cys Gly His Cys Asp Ser Lys Asn Arg Asn Gly Val Arg Phe Leu Cys 
    690                 695                 700                 


Lys Gly Cys Gly Ala Ser Met Asp Ala Asp Phe Asp Ala Ala Cys Arg 
705                 710                 715                 720 


Asn Leu Glu Arg Val Ala Leu Thr Gly Lys Pro Met Pro Lys Pro Ser 
                725                 730                 735     


Thr Ser Cys Glu Arg Leu Leu Ser Ala Thr Thr Gly Lys Val Cys Ser 
            740                 745                 750         


Asp His Ser Leu Ser His Asp Ala Ile Glu Lys Ala Ser 
        755                 760                 765 


<210>  226
<211>  793
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-5 polypeptide sequence

<400>  226

Met Ser Ser Leu Pro Thr Pro Leu Glu Leu Leu Lys Gln Lys His Ala 
1               5                   10                  15      


Asp Leu Phe Lys Gly Leu Gln Phe Ser Ser Lys Asp Asn Lys Met Ala 
            20                  25                  30          


Gly Lys Val Leu Lys Lys Asp Gly Glu Glu Ala Ala Leu Ala Phe Leu 
        35                  40                  45              


Ser Glu Arg Gly Val Ser Arg Gly Glu Leu Pro Asn Phe Arg Pro Pro 
    50                  55                  60                  


Ala Lys Thr Leu Val Val Ala Gln Ser Arg Pro Phe Glu Glu Phe Pro 
65                  70                  75                  80  


Ile Tyr Arg Val Ser Glu Ala Ile Gln Leu Tyr Val Tyr Ser Leu Ser 
                85                  90                  95      


Val Lys Glu Leu Glu Thr Val Pro Ser Gly Ser Ser Thr Lys Lys Glu 
            100                 105                 110         


His Gln Arg Phe Phe Gln Asp Ser Ser Val Pro Asp Phe Gly Tyr Thr 
        115                 120                 125             


Ser Val Gln Gly Leu Asn Lys Ile Phe Gly Leu Ala Arg Gly Ile Tyr 
    130                 135                 140                 


Leu Gly Val Ile Thr Arg Gly Glu Asn Gln Leu Gln Lys Ala Lys Ser 
145                 150                 155                 160 


Lys His Glu Ala Leu Asn Lys Lys Arg Arg Ala Ser Gly Glu Ala Glu 
                165                 170                 175     


Thr Glu Phe Asp Pro Thr Pro Tyr Glu Tyr Met Thr Pro Glu Arg Lys 
            180                 185                 190         


Leu Ala Lys Pro Pro Gly Val Asn His Ser Ile Met Cys Tyr Val Asp 
        195                 200                 205             


Ile Ser Val Asp Glu Phe Asp Phe Arg Asn Pro Asp Gly Ile Val Leu 
    210                 215                 220                 


Pro Ser Glu Tyr Ala Gly Tyr Cys Arg Glu Ile Asn Thr Ala Ile Glu 
225                 230                 235                 240 


Lys Gly Thr Val Asp Arg Leu Gly His Leu Lys Gly Gly Pro Gly Tyr 
                245                 250                 255     


Ile Pro Gly His Gln Arg Lys Glu Ser Thr Thr Glu Gly Pro Lys Ile 
            260                 265                 270         


Asn Phe Arg Lys Gly Arg Ile Arg Arg Ser Tyr Thr Ala Leu Tyr Ala 
        275                 280                 285             


Lys Arg Asp Ser Arg Arg Val Arg Gln Gly Lys Leu Ala Leu Pro Ser 
    290                 295                 300                 


Tyr Arg His His Met Met Arg Leu Asn Ser Asn Ala Glu Ser Ala Ile 
305                 310                 315                 320 


Leu Ala Val Ile Phe Phe Gly Lys Asp Trp Val Val Phe Asp Leu Arg 
                325                 330                 335     


Gly Leu Leu Arg Asn Val Arg Trp Arg Asn Leu Phe Val Asp Gly Ser 
            340                 345                 350         


Thr Pro Ser Thr Leu Leu Gly Met Phe Gly Asp Pro Val Ile Asp Pro 
        355                 360                 365             


Lys Arg Gly Val Val Ala Phe Cys Tyr Lys Glu Gln Ile Val Pro Val 
    370                 375                 380                 


Val Ser Lys Ser Ile Thr Lys Met Val Lys Ala Pro Glu Leu Leu Asn 
385                 390                 395                 400 


Lys Leu Tyr Leu Lys Ser Glu Asp Pro Leu Val Leu Val Ala Ile Asp 
                405                 410                 415     


Leu Gly Gln Thr Asn Pro Val Gly Val Gly Val Tyr Arg Val Met Asn 
            420                 425                 430         


Ala Ser Leu Asp Tyr Glu Val Val Thr Arg Phe Ala Leu Glu Ser Glu 
        435                 440                 445             


Leu Leu Arg Glu Ile Glu Ser Tyr Arg Gln Arg Thr Asn Ala Phe Glu 
    450                 455                 460                 


Ala Gln Ile Arg Ala Glu Thr Phe Asp Ala Met Thr Ser Glu Glu Gln 
465                 470                 475                 480 


Glu Glu Ile Thr Arg Val Arg Ala Phe Ser Ala Ser Lys Ala Lys Glu 
                485                 490                 495     


Asn Val Cys His Arg Phe Gly Met Pro Val Asp Ala Val Asp Trp Ala 
            500                 505                 510         


Thr Met Gly Ser Asn Thr Ile His Ile Ala Lys Trp Val Met Arg His 
        515                 520                 525             


Gly Asp Pro Ser Leu Val Glu Val Leu Glu Tyr Arg Lys Asp Asn Glu 
    530                 535                 540                 


Ile Lys Leu Asp Lys Asn Gly Val Pro Lys Lys Val Lys Leu Thr Asp 
545                 550                 555                 560 


Lys Arg Ile Ala Asn Leu Thr Ser Ile Arg Leu Arg Phe Ser Gln Glu 
                565                 570                 575     


Thr Ser Lys His Tyr Asn Asp Thr Met Trp Glu Leu Arg Arg Lys His 
            580                 585                 590         


Pro Val Tyr Gln Lys Leu Ser Lys Ser Lys Ala Asp Phe Ser Arg Arg 
        595                 600                 605             


Val Val Asn Ser Ile Ile Arg Arg Val Asn His Leu Val Pro Arg Ala 
    610                 615                 620                 


Arg Ile Val Phe Ile Ile Glu Asp Leu Lys Asn Leu Gly Lys Val Phe 
625                 630                 635                 640 


His Gly Ser Gly Lys Arg Glu Leu Gly Trp Asp Ser Tyr Phe Glu Pro 
                645                 650                 655     


Lys Ser Glu Asn Arg Trp Phe Ile Gln Val Leu His Lys Ala Phe Ser 
            660                 665                 670         


Glu Thr Gly Lys His Lys Gly Tyr Tyr Ile Ile Glu Cys Trp Pro Asn 
        675                 680                 685             


Trp Thr Ser Cys Thr Cys Pro Lys Cys Ser Cys Cys Asp Ser Glu Asn 
    690                 695                 700                 


Arg His Gly Glu Val Phe Arg Cys Leu Ala Cys Gly Tyr Thr Cys Asn 
705                 710                 715                 720 


Thr Asp Phe Gly Thr Ala Pro Asp Asn Leu Val Lys Ile Ala Thr Thr 
                725                 730                 735     


Gly Lys Gly Leu Pro Gly Pro Lys Lys Arg Cys Lys Gly Ser Ser Lys 
            740                 745                 750         


Gly Lys Asn Pro Lys Ile Ala Arg Ser Ser Glu Thr Gly Val Ser Val 
        755                 760                 765             


Thr Glu Ser Gly Ala Pro Lys Val Lys Lys Ser Ser Pro Thr Gln Thr 
    770                 775                 780                 


Ser Gln Ser Ser Ser Gln Ser Ala Pro 
785                 790             


<210>  227
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-6 polypeptide sequence

<400>  227

Met Asn Lys Ile Glu Lys Glu Lys Thr Pro Leu Ala Lys Leu Met Asn 
1               5                   10                  15      


Glu Asn Phe Ala Gly Leu Arg Phe Pro Phe Ala Ile Ile Lys Gln Ala 
            20                  25                  30          


Gly Lys Lys Leu Leu Lys Glu Gly Glu Leu Lys Thr Ile Glu Tyr Met 
        35                  40                  45              


Thr Gly Lys Gly Ser Ile Glu Pro Leu Pro Asn Phe Lys Pro Pro Val 
    50                  55                  60                  


Lys Cys Leu Ile Val Ala Lys Arg Arg Asp Leu Lys Tyr Phe Pro Ile 
65                  70                  75                  80  


Cys Lys Ala Ser Cys Glu Ile Gln Ser Tyr Val Tyr Ser Leu Asn Tyr 
                85                  90                  95      


Lys Asp Phe Met Asp Tyr Phe Ser Thr Pro Met Thr Ser Gln Lys Gln 
            100                 105                 110         


His Glu Glu Phe Phe Lys Lys Ser Gly Leu Asn Ile Glu Tyr Gln Asn 
        115                 120                 125             


Val Ala Gly Leu Asn Leu Ile Phe Asn Asn Val Lys Asn Thr Tyr Asn 
    130                 135                 140                 


Gly Val Ile Leu Lys Val Lys Asn Arg Asn Glu Lys Leu Lys Lys Lys 
145                 150                 155                 160 


Ala Ile Lys Asn Asn Tyr Glu Phe Glu Glu Ile Lys Thr Phe Asn Asp 
                165                 170                 175     


Asp Gly Cys Leu Ile Asn Lys Pro Gly Ile Asn Asn Val Ile Tyr Cys 
            180                 185                 190         


Phe Gln Ser Ile Ser Pro Lys Ile Leu Lys Asn Ile Thr His Leu Pro 
        195                 200                 205             


Lys Glu Tyr Asn Asp Tyr Asp Cys Ser Val Asp Arg Asn Ile Ile Gln 
    210                 215                 220                 


Lys Tyr Val Ser Arg Leu Asp Ile Pro Glu Ser Gln Pro Gly His Val 
225                 230                 235                 240 


Pro Glu Trp Gln Arg Lys Leu Pro Glu Phe Asn Asn Thr Asn Asn Pro 
                245                 250                 255     


Arg Arg Arg Arg Lys Trp Tyr Ser Asn Gly Arg Asn Ile Ser Lys Gly 
            260                 265                 270         


Tyr Ser Val Asp Gln Val Asn Gln Ala Lys Ile Glu Asp Ser Leu Leu 
        275                 280                 285             


Ala Gln Ile Lys Ile Gly Glu Asp Trp Ile Ile Leu Asp Ile Arg Gly 
    290                 295                 300                 


Leu Leu Arg Asp Leu Asn Arg Arg Glu Leu Ile Ser Tyr Lys Asn Lys 
305                 310                 315                 320 


Leu Thr Ile Lys Asp Val Leu Gly Phe Phe Ser Asp Tyr Pro Ile Ile 
                325                 330                 335     


Asp Ile Lys Lys Asn Leu Val Thr Phe Cys Tyr Lys Glu Gly Val Ile 
            340                 345                 350         


Gln Val Val Ser Gln Lys Ser Ile Gly Asn Lys Lys Ser Lys Gln Leu 
        355                 360                 365             


Leu Glu Lys Leu Ile Glu Asn Lys Pro Ile Ala Leu Val Ser Ile Asp 
    370                 375                 380                 


Leu Gly Gln Thr Asn Pro Val Ser Val Lys Ile Ser Lys Leu Asn Lys 
385                 390                 395                 400 


Ile Asn Asn Lys Ile Ser Ile Glu Ser Phe Thr Tyr Arg Phe Leu Asn 
                405                 410                 415     


Glu Glu Ile Leu Lys Glu Ile Glu Lys Tyr Arg Lys Asp Tyr Asp Lys 
            420                 425                 430         


Leu Glu Leu Lys Leu Ile Asn Glu Ala 
        435                 440     


<210>  228
<211>  772
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-7 polypeptide sequence

<400>  228

Met Ser Asn Thr Ala Val Ser Thr Arg Glu His Met Ser Asn Lys Thr 
1               5                   10                  15      


Thr Pro Pro Ser Pro Leu Ser Leu Leu Leu Arg Ala His Phe Pro Gly 
            20                  25                  30          


Leu Lys Phe Glu Ser Gln Asp Tyr Lys Ile Ala Gly Lys Lys Leu Arg 
        35                  40                  45              


Asp Gly Gly Pro Glu Ala Val Ile Ser Tyr Leu Thr Gly Lys Gly Gln 
    50                  55                  60                  


Ala Lys Leu Lys Asp Val Lys Pro Pro Ala Lys Ala Phe Val Ile Ala 
65                  70                  75                  80  


Gln Ser Arg Pro Phe Ile Glu Trp Asp Leu Val Arg Val Ser Arg Gln 
                85                  90                  95      


Ile Gln Glu Lys Ile Phe Gly Ile Pro Ala Thr Lys Gly Arg Pro Lys 
            100                 105                 110         


Gln Asp Gly Leu Ser Glu Thr Ala Phe Asn Glu Ala Val Ala Ser Leu 
        115                 120                 125             


Glu Val Asp Gly Lys Ser Lys Leu Asn Glu Glu Thr Arg Ala Ala Phe 
    130                 135                 140                 


Tyr Glu Val Leu Gly Leu Asp Ala Pro Ser Leu His Ala Gln Ala Gln 
145                 150                 155                 160 


Asn Ala Leu Ile Lys Ser Ala Ile Ser Ile Arg Glu Gly Val Leu Lys 
                165                 170                 175     


Lys Val Glu Asn Arg Asn Glu Lys Asn Leu Ser Lys Thr Lys Arg Arg 
            180                 185                 190         


Lys Glu Ala Gly Glu Glu Ala Thr Phe Val Glu Glu Lys Ala His Asp 
        195                 200                 205             


Glu Arg Gly Tyr Leu Ile His Pro Pro Gly Val Asn Gln Thr Ile Pro 
    210                 215                 220                 


Gly Tyr Gln Ala Val Val Ile Lys Ser Cys Pro Ser Asp Phe Ile Gly 
225                 230                 235                 240 


Leu Pro Ser Gly Cys Leu Ala Lys Glu Ser Ala Glu Ala Leu Thr Asp 
                245                 250                 255     


Tyr Leu Pro His Asp Arg Met Thr Ile Pro Lys Gly Gln Pro Gly Tyr 
            260                 265                 270         


Val Pro Glu Trp Gln His Pro Leu Leu Asn Arg Arg Lys Asn Arg Arg 
        275                 280                 285             


Arg Arg Asp Trp Tyr Ser Ala Ser Leu Asn Lys Pro Lys Ala Thr Cys 
    290                 295                 300                 


Ser Lys Arg Ser Gly Thr Pro Asn Arg Lys Asn Ser Arg Thr Asp Gln 
305                 310                 315                 320 


Ile Gln Ser Gly Arg Phe Lys Gly Ala Ile Pro Val Leu Met Arg Phe 
                325                 330                 335     


Gln Asp Glu Trp Val Ile Ile Asp Ile Arg Gly Leu Leu Arg Asn Ala 
            340                 345                 350         


Arg Tyr Arg Lys Leu Leu Lys Glu Lys Ser Thr Ile Pro Asp Leu Leu 
        355                 360                 365             


Ser Leu Phe Thr Gly Asp Pro Ser Ile Asp Met Arg Gln Gly Val Cys 
    370                 375                 380                 


Thr Phe Ile Tyr Lys Ala Gly Gln Ala Cys Ser Ala Lys Met Val Lys 
385                 390                 395                 400 


Thr Lys Asn Ala Pro Glu Ile Leu Ser Glu Leu Thr Lys Ser Gly Pro 
                405                 410                 415     


Val Val Leu Val Ser Ile Asp Leu Gly Gln Thr Asn Pro Ile Ala Ala 
            420                 425                 430         


Lys Val Ser Arg Val Thr Gln Leu Ser Asp Gly Gln Leu Ser His Glu 
        435                 440                 445             


Thr Leu Leu Arg Glu Leu Leu Ser Asn Asp Ser Ser Asp Gly Lys Glu 
    450                 455                 460                 


Ile Ala Arg Tyr Arg Val Ala Ser Asp Arg Leu Arg Asp Lys Leu Ala 
465                 470                 475                 480 


Asn Leu Ala Val Glu Arg Leu Ser Pro Glu His Lys Ser Glu Ile Leu 
                485                 490                 495     


Arg Ala Lys Asn Asp Thr Pro Ala Leu Cys Lys Ala Arg Val Cys Ala 
            500                 505                 510         


Ala Leu Gly Leu Asn Pro Glu Met Ile Ala Trp Asp Lys Met Thr Pro 
        515                 520                 525             


Tyr Thr Glu Phe Leu Ala Thr Ala Tyr Leu Glu Lys Gly Gly Asp Arg 
    530                 535                 540                 


Lys Val Ala Thr Leu Lys Pro Lys Asn Arg Pro Glu Met Leu Arg Arg 
545                 550                 555                 560 


Asp Ile Lys Phe Lys Gly Thr Glu Gly Val Arg Ile Glu Val Ser Pro 
                565                 570                 575     


Glu Ala Ala Glu Ala Tyr Arg Glu Ala Gln Trp Asp Leu Gln Arg Thr 
            580                 585                 590         


Ser Pro Glu Tyr Leu Arg Leu Ser Thr Trp Lys Gln Glu Leu Thr Lys 
        595                 600                 605             


Arg Ile Leu Asn Gln Leu Arg His Lys Ala Ala Lys Ser Ser Gln Cys 
    610                 615                 620                 


Glu Val Val Val Met Ala Phe Glu Asp Leu Asn Ile Lys Met Met His 
625                 630                 635                 640 


Gly Asn Gly Lys Trp Ala Asp Gly Gly Trp Asp Ala Phe Phe Ile Lys 
                645                 650                 655     


Lys Arg Glu Asn Arg Trp Phe Met Gln Ala Phe His Lys Ser Leu Thr 
            660                 665                 670         


Glu Leu Gly Ala His Lys Gly Val Pro Thr Ile Glu Val Thr Pro His 
        675                 680                 685             


Arg Thr Ser Ile Thr Cys Thr Lys Cys Gly His Cys Asp Lys Ala Asn 
    690                 695                 700                 


Arg Asp Gly Glu Arg Phe Ala Cys Gln Lys Cys Gly Phe Val Ala His 
705                 710                 715                 720 


Ala Asp Leu Glu Ile Ala Thr Asp Asn Ile Glu Arg Val Ala Leu Thr 
                725                 730                 735     


Gly Lys Pro Met Pro Lys Pro Glu Ser Glu Arg Ser Gly Asp Ala Lys 
            740                 745                 750         


Lys Ser Val Gly Ala Arg Lys Ala Ala Phe Lys Pro Glu Glu Asp Ala 
        755                 760                 765             


Glu Ala Ala Glu 
    770         


<210>  229
<211>  717
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-8 polypeptide sequence

<400>  229

Met Ile Lys Pro Thr Val Ser Gln Phe Leu Thr Pro Gly Phe Lys Leu 
1               5                   10                  15      


Ile Arg Asn His Ser Arg Thr Ala Gly Leu Lys Leu Lys Asn Glu Gly 
            20                  25                  30          


Glu Glu Ala Cys Lys Lys Phe Val Arg Glu Asn Glu Ile Pro Lys Asp 
        35                  40                  45              


Glu Cys Pro Asn Phe Gln Gly Gly Pro Ala Ile Ala Asn Ile Ile Ala 
    50                  55                  60                  


Lys Ser Arg Glu Phe Thr Glu Trp Glu Ile Tyr Gln Ser Ser Leu Ala 
65                  70                  75                  80  


Ile Gln Glu Val Ile Phe Thr Leu Pro Lys Asp Lys Leu Pro Glu Pro 
                85                  90                  95      


Ile Leu Lys Glu Glu Trp Arg Ala Gln Trp Leu Ser Glu His Gly Leu 
            100                 105                 110         


Asp Thr Val Pro Tyr Lys Glu Ala Ala Gly Leu Asn Leu Ile Ile Lys 
        115                 120                 125             


Asn Ala Val Asn Thr Tyr Lys Gly Val Gln Val Lys Val Asp Asn Lys 
    130                 135                 140                 


Asn Lys Asn Asn Leu Ala Lys Ile Asn Arg Lys Asn Glu Ile Ala Lys 
145                 150                 155                 160 


Leu Asn Gly Glu Gln Glu Ile Ser Phe Glu Glu Ile Lys Ala Phe Asp 
                165                 170                 175     


Asp Lys Gly Tyr Leu Leu Gln Lys Pro Ser Pro Asn Lys Ser Ile Tyr 
            180                 185                 190         


Cys Tyr Gln Ser Val Ser Pro Lys Pro Phe Ile Thr Ser Lys Tyr His 
        195                 200                 205             


Asn Val Asn Leu Pro Glu Glu Tyr Ile Gly Tyr Tyr Arg Lys Ser Asn 
    210                 215                 220                 


Glu Pro Ile Val Ser Pro Tyr Gln Phe Asp Arg Leu Arg Ile Pro Ile 
225                 230                 235                 240 


Gly Glu Pro Gly Tyr Val Pro Lys Trp Gln Tyr Thr Phe Leu Ser Lys 
                245                 250                 255     


Lys Glu Asn Lys Arg Arg Lys Leu Ser Lys Arg Ile Lys Asn Val Ser 
            260                 265                 270         


Pro Ile Leu Gly Ile Ile Cys Ile Lys Lys Asp Trp Cys Val Phe Asp 
        275                 280                 285             


Met Arg Gly Leu Leu Arg Thr Asn His Trp Lys Lys Tyr His Lys Pro 
    290                 295                 300                 


Thr Asp Ser Ile Asn Asp Leu Phe Asp Tyr Phe Thr Gly Asp Pro Val 
305                 310                 315                 320 


Ile Asp Thr Lys Ala Asn Val Val Arg Phe Arg Tyr Lys Met Glu Asn 
                325                 330                 335     


Gly Ile Val Asn Tyr Lys Pro Val Arg Glu Lys Lys Gly Lys Glu Leu 
            340                 345                 350         


Leu Glu Asn Ile Cys Asp Gln Asn Gly Ser Cys Lys Leu Ala Thr Val 
        355                 360                 365             


Asp Val Gly Gln Asn Asn Pro Val Ala Ile Gly Leu Phe Glu Leu Lys 
    370                 375                 380                 


Lys Val Asn Gly Glu Leu Thr Lys Thr Leu Ile Ser Arg His Pro Thr 
385                 390                 395                 400 


Pro Ile Asp Phe Cys Asn Lys Ile Thr Ala Tyr Arg Glu Arg Tyr Asp 
                405                 410                 415     


Lys Leu Glu Ser Ser Ile Lys Leu Asp Ala Ile Lys Gln Leu Thr Ser 
            420                 425                 430         


Glu Gln Lys Ile Glu Val Asp Asn Tyr Asn Asn Asn Phe Thr Pro Gln 
        435                 440                 445             


Asn Thr Lys Gln Ile Val Cys Ser Lys Leu Asn Ile Asn Pro Asn Asp 
    450                 455                 460                 


Leu Pro Trp Asp Lys Met Ile Ser Gly Thr His Phe Ile Ser Glu Lys 
465                 470                 475                 480 


Ala Gln Val Ser Asn Lys Ser Glu Ile Tyr Phe Thr Ser Thr Asp Lys 
                485                 490                 495     


Gly Lys Thr Lys Asp Val Met Lys Ser Asp Tyr Lys Trp Phe Gln Asp 
            500                 505                 510         


Tyr Lys Pro Lys Leu Ser Lys Glu Val Arg Asp Ala Leu Ser Asp Ile 
        515                 520                 525             


Glu Trp Arg Leu Arg Arg Glu Ser Leu Glu Phe Asn Lys Leu Ser Lys 
    530                 535                 540                 


Ser Arg Glu Gln Asp Ala Arg Gln Leu Ala Asn Trp Ile Ser Ser Met 
545                 550                 555                 560 


Cys Asp Val Ile Gly Ile Glu Asn Leu Val Lys Lys Asn Asn Phe Phe 
                565                 570                 575     


Gly Gly Ser Gly Lys Arg Glu Pro Gly Trp Asp Asn Phe Tyr Lys Pro 
            580                 585                 590         


Lys Lys Glu Asn Arg Trp Trp Ile Asn Ala Ile His Lys Ala Leu Thr 
        595                 600                 605             


Glu Leu Ser Gln Asn Lys Gly Lys Arg Val Ile Leu Leu Pro Ala Met 
    610                 615                 620                 


Arg Thr Ser Ile Thr Cys Pro Lys Cys Lys Tyr Cys Asp Ser Lys Asn 
625                 630                 635                 640 


Arg Asn Gly Glu Lys Phe Asn Cys Leu Lys Cys Gly Ile Glu Leu Asn 
                645                 650                 655     


Ala Asp Ile Asp Val Ala Thr Glu Asn Leu Ala Thr Val Ala Ile Thr 
            660                 665                 670         


Ala Gln Ser Met Pro Lys Pro Thr Cys Glu Arg Ser Gly Asp Ala Lys 
        675                 680                 685             


Lys Pro Val Arg Ala Arg Lys Ala Lys Ala Pro Glu Phe His Asp Lys 
    690                 695                 700                 


Leu Ala Pro Ser Tyr Thr Val Val Leu Arg Glu Ala Val 
705                 710                 715         


<210>  230
<211>  793
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-9 polypeptide sequence

<400>  230

Met Arg Ser Ser Arg Glu Ile Gly Asp Lys Ile Leu Met Arg Gln Pro 
1               5                   10                  15      


Ala Glu Lys Thr Ala Phe Gln Val Phe Arg Gln Glu Val Ile Gly Thr 
            20                  25                  30          


Gln Lys Leu Ser Gly Gly Asp Ala Lys Thr Ala Gly Arg Leu Tyr Lys 
        35                  40                  45              


Gln Gly Lys Met Glu Ala Ala Arg Glu Trp Leu Leu Lys Gly Ala Arg 
    50                  55                  60                  


Asp Asp Val Pro Pro Asn Phe Gln Pro Pro Ala Lys Cys Leu Val Val 
65                  70                  75                  80  


Ala Val Ser His Pro Phe Glu Glu Trp Asp Ile Ser Lys Thr Asn His 
                85                  90                  95      


Asp Val Gln Ala Tyr Ile Tyr Ala Gln Pro Leu Gln Ala Glu Gly His 
            100                 105                 110         


Leu Asn Gly Leu Ser Glu Lys Trp Glu Asp Thr Ser Ala Asp Gln His 
        115                 120                 125             


Lys Leu Trp Phe Glu Lys Thr Gly Val Pro Asp Arg Gly Leu Pro Val 
    130                 135                 140                 


Gln Ala Ile Asn Lys Ile Ala Lys Ala Ala Val Asn Arg Ala Phe Gly 
145                 150                 155                 160 


Val Val Arg Lys Val Glu Asn Arg Asn Glu Lys Arg Arg Ser Arg Asp 
                165                 170                 175     


Asn Arg Ile Ala Glu His Asn Arg Glu Asn Gly Leu Thr Glu Val Val 
            180                 185                 190         


Arg Glu Ala Pro Glu Val Ala Thr Asn Ala Asp Gly Phe Leu Leu His 
        195                 200                 205             


Pro Pro Gly Ile Asp Pro Ser Ile Leu Ser Tyr Ala Ser Val Ser Pro 
    210                 215                 220                 


Val Pro Tyr Asn Ser Ser Lys His Ser Phe Val Arg Leu Pro Glu Glu 
225                 230                 235                 240 


Tyr Gln Ala Tyr Asn Val Glu Pro Asp Ala Pro Ile Pro Gln Phe Val 
                245                 250                 255     


Val Glu Asp Arg Phe Ala Ile Pro Pro Gly Gln Pro Gly Tyr Val Pro 
            260                 265                 270         


Glu Trp Gln Arg Leu Lys Cys Ser Thr Asn Lys His Arg Arg Met Arg 
        275                 280                 285             


Gln Trp Ser Asn Gln Asp Tyr Lys Pro Lys Ala Gly Arg Arg Ala Lys 
    290                 295                 300                 


Pro Leu Glu Phe Gln Ala His Leu Thr Arg Glu Arg Ala Lys Gly Ala 
305                 310                 315                 320 


Leu Leu Val Val Met Arg Ile Lys Glu Asp Trp Val Val Phe Asp Val 
                325                 330                 335     


Arg Gly Leu Leu Arg Asn Val Glu Trp Arg Lys Val Leu Ser Glu Glu 
            340                 345                 350         


Ala Arg Glu Lys Leu Thr Leu Lys Gly Leu Leu Asp Leu Phe Thr Gly 
        355                 360                 365             


Asp Pro Val Ile Asp Thr Lys Arg Gly Ile Val Thr Phe Leu Tyr Lys 
    370                 375                 380                 


Ala Glu Ile Thr Lys Ile Leu Ser Lys Arg Thr Val Lys Thr Lys Asn 
385                 390                 395                 400 


Ala Arg Asp Leu Leu Leu Arg Leu Thr Glu Pro Gly Glu Asp Gly Leu 
                405                 410                 415     


Arg Arg Glu Val Gly Leu Val Ala Val Asp Leu Gly Gln Thr His Pro 
            420                 425                 430         


Ile Ala Ala Ala Ile Tyr Arg Ile Gly Arg Thr Ser Ala Gly Ala Leu 
        435                 440                 445             


Glu Ser Thr Val Leu His Arg Gln Gly Leu Arg Glu Asp Gln Lys Glu 
    450                 455                 460                 


Lys Leu Lys Glu Tyr Arg Lys Arg His Thr Ala Leu Asp Ser Arg Leu 
465                 470                 475                 480 


Arg Lys Glu Ala Phe Glu Thr Leu Ser Val Glu Gln Gln Lys Glu Ile 
                485                 490                 495     


Val Thr Val Ser Gly Ser Gly Ala Gln Ile Thr Lys Asp Lys Val Cys 
            500                 505                 510         


Asn Tyr Leu Gly Val Asp Pro Ser Thr Leu Pro Trp Glu Lys Met Gly 
        515                 520                 525             


Ser Tyr Thr His Phe Ile Ser Asp Asp Phe Leu Arg Arg Gly Gly Asp 
    530                 535                 540                 


Pro Asn Ile Val His Phe Asp Arg Gln Pro Lys Lys Gly Lys Val Ser 
545                 550                 555                 560 


Lys Lys Ser Gln Arg Ile Lys Arg Ser Asp Ser Gln Trp Val Gly Arg 
                565                 570                 575     


Met Arg Pro Arg Leu Ser Gln Glu Thr Ala Lys Ala Arg Met Glu Ala 
            580                 585                 590         


Asp Trp Ala Ala Gln Asn Glu Asn Glu Glu Tyr Lys Arg Leu Ala Arg 
        595                 600                 605             


Ser Lys Gln Glu Leu Ala Arg Trp Cys Val Asn Thr Leu Leu Gln Asn 
    610                 615                 620                 


Thr Arg Cys Ile Thr Gln Cys Asp Glu Ile Val Val Val Ile Glu Asp 
625                 630                 635                 640 


Leu Asn Val Lys Ser Leu His Gly Lys Gly Ala Arg Glu Pro Gly Trp 
                645                 650                 655     


Asp Asn Phe Phe Thr Pro Lys Thr Glu Asn Arg Trp Phe Ile Gln Ile 
            660                 665                 670         


Leu His Lys Thr Phe Ser Glu Leu Pro Lys His Arg Gly Glu His Val 
        675                 680                 685             


Ile Glu Gly Cys Pro Leu Arg Thr Ser Ile Thr Cys Pro Ala Cys Ser 
    690                 695                 700                 


Tyr Cys Asp Lys Asn Ser Arg Asn Gly Glu Lys Phe Val Cys Val Ala 
705                 710                 715                 720 


Cys Gly Ala Thr Phe His Ala Asp Phe Glu Val Ala Thr Tyr Asn Leu 
                725                 730                 735     


Val Arg Leu Ala Thr Thr Gly Met Pro Met Pro Lys Ser Leu Glu Arg 
            740                 745                 750         


Gln Gly Gly Gly Glu Lys Ala Gly Gly Ala Arg Lys Ala Arg Lys Lys 
        755                 760                 765             


Ala Lys Gln Val Glu Lys Ile Val Val Gln Ala Asn Ala Asn Val Thr 
    770                 775                 780                 


Met Asn Gly Ala Ser Leu His Ser Pro 
785                 790             


<210>  231
<211>  812
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Casphi-10 polypeptide 
       sequence

<400>  231

Met Asp Met Leu Asp Thr Glu Thr Asn Tyr Ala Thr Glu Thr Pro Ala 
1               5                   10                  15      


Gln Gln Gln Asp Tyr Ser Pro Lys Pro Pro Lys Lys Ala Gln Arg Ala 
            20                  25                  30          


Pro Lys Gly Phe Ser Lys Lys Ala Arg Pro Glu Lys Lys Pro Pro Lys 
        35                  40                  45              


Pro Ile Thr Leu Phe Thr Gln Lys His Phe Ser Gly Val Arg Phe Leu 
    50                  55                  60                  


Lys Arg Val Ile Arg Asp Ala Ser Lys Ile Leu Lys Leu Ser Glu Ser 
65                  70                  75                  80  


Arg Thr Ile Thr Phe Leu Glu Gln Ala Ile Glu Arg Asp Gly Ser Ala 
                85                  90                  95      


Pro Pro Asp Val Thr Pro Pro Val His Asn Thr Ile Met Ala Val Thr 
            100                 105                 110         


Arg Pro Phe Glu Glu Trp Pro Glu Val Ile Leu Ser Lys Ala Leu Gln 
        115                 120                 125             


Lys His Cys Tyr Ala Leu Thr Lys Lys Ile Lys Ile Lys Thr Trp Pro 
    130                 135                 140                 


Lys Lys Gly Pro Gly Lys Lys Cys Leu Ala Ala Trp Ser Ala Arg Thr 
145                 150                 155                 160 


Lys Ile Pro Leu Ile Pro Gly Gln Val Gln Ala Thr Asn Gly Leu Phe 
                165                 170                 175     


Asp Arg Ile Gly Ser Ile Tyr Asp Gly Val Glu Lys Lys Val Thr Asn 
            180                 185                 190         


Arg Asn Ala Asn Lys Lys Leu Glu Tyr Asp Glu Ala Ile Lys Glu Gly 
        195                 200                 205             


Arg Asn Pro Ala Val Pro Glu Tyr Glu Thr Ala Tyr Asn Ile Asp Gly 
    210                 215                 220                 


Thr Leu Ile Asn Lys Pro Gly Tyr Asn Pro Asn Leu Tyr Ile Thr Gln 
225                 230                 235                 240 


Ser Arg Thr Pro Arg Leu Ile Thr Glu Ala Asp Arg Pro Leu Val Glu 
                245                 250                 255     


Lys Ile Leu Trp Gln Met Val Glu Lys Lys Thr Gln Ser Arg Asn Gln 
            260                 265                 270         


Ala Arg Arg Ala Arg Leu Glu Lys Ala Ala His Leu Gln Gly Leu Pro 
        275                 280                 285             


Val Pro Lys Phe Val Pro Glu Lys Val Asp Arg Ser Gln Lys Ile Glu 
    290                 295                 300                 


Ile Arg Ile Ile Asp Pro Leu Asp Lys Ile Glu Pro Tyr Met Pro Gln 
305                 310                 315                 320 


Asp Arg Met Ala Ile Lys Ala Ser Gln Asp Gly His Val Pro Tyr Trp 
                325                 330                 335     


Gln Arg Pro Phe Leu Ser Lys Arg Arg Asn Arg Arg Val Arg Ala Gly 
            340                 345                 350         


Trp Gly Lys Gln Val Ser Ser Ile Gln Ala Trp Leu Thr Gly Ala Leu 
        355                 360                 365             


Leu Val Ile Val Arg Leu Gly Asn Glu Ala Phe Leu Ala Asp Ile Arg 
    370                 375                 380                 


Gly Ala Leu Arg Asn Ala Gln Trp Arg Lys Leu Leu Lys Pro Asp Ala 
385                 390                 395                 400 


Thr Tyr Gln Ser Leu Phe Asn Leu Phe Thr Gly Asp Pro Val Val Asn 
                405                 410                 415     


Thr Arg Thr Asn His Leu Thr Met Ala Tyr Arg Glu Gly Val Val Asn 
            420                 425                 430         


Ile Val Lys Ser Arg Ser Phe Lys Gly Arg Gln Thr Arg Glu His Leu 
        435                 440                 445             


Leu Thr Leu Leu Gly Gln Gly Lys Thr Val Ala Gly Val Ser Phe Asp 
    450                 455                 460                 


Leu Gly Gln Lys His Ala Ala Gly Leu Leu Ala Ala His Phe Gly Leu 
465                 470                 475                 480 


Gly Glu Asp Gly Asn Pro Val Phe Thr Pro Ile Gln Ala Cys Phe Leu 
                485                 490                 495     


Pro Gln Arg Tyr Leu Asp Ser Leu Thr Asn Tyr Arg Asn Arg Tyr Asp 
            500                 505                 510         


Ala Leu Thr Leu Asp Met Arg Arg Gln Ser Leu Leu Ala Leu Thr Pro 
        515                 520                 525             


Ala Gln Gln Gln Glu Phe Ala Asp Ala Gln Arg Asp Pro Gly Gly Gln 
    530                 535                 540                 


Ala Lys Arg Ala Cys Cys Leu Lys Leu Asn Leu Asn Pro Asp Glu Ile 
545                 550                 555                 560 


Arg Trp Asp Leu Val Ser Gly Ile Ser Thr Met Ile Ser Asp Leu Tyr 
                565                 570                 575     


Ile Glu Arg Gly Gly Asp Pro Arg Asp Val His Gln Gln Val Glu Thr 
            580                 585                 590         


Lys Pro Lys Gly Lys Arg Lys Ser Glu Ile Arg Ile Leu Lys Ile Arg 
        595                 600                 605             


Asp Gly Lys Trp Ala Tyr Asp Phe Arg Pro Lys Ile Ala Asp Glu Thr 
    610                 615                 620                 


Arg Lys Ala Gln Arg Glu Gln Leu Trp Lys Leu Gln Lys Ala Ser Ser 
625                 630                 635                 640 


Glu Phe Glu Arg Leu Ser Arg Tyr Lys Ile Asn Ile Ala Arg Ala Ile 
                645                 650                 655     


Ala Asn Trp Ala Leu Gln Trp Gly Arg Glu Leu Ser Gly Cys Asp Ile 
            660                 665                 670         


Val Ile Pro Val Leu Glu Asp Leu Asn Val Gly Ser Lys Phe Phe Asp 
        675                 680                 685             


Gly Lys Gly Lys Trp Leu Leu Gly Trp Asp Asn Arg Phe Thr Pro Lys 
    690                 695                 700                 


Lys Glu Asn Arg Trp Phe Ile Lys Val Leu His Lys Ala Val Ala Glu 
705                 710                 715                 720 


Leu Ala Pro His Arg Gly Val Pro Val Tyr Glu Val Met Pro His Arg 
                725                 730                 735     


Thr Ser Met Thr Cys Pro Ala Cys His Tyr Cys His Pro Thr Asn Arg 
            740                 745                 750         


Glu Gly Asp Arg Phe Glu Cys Gln Ser Cys His Val Val Lys Asn Thr 
        755                 760                 765             


Asp Arg Asp Val Ala Pro Tyr Asn Ile Leu Arg Val Ala Val Glu Gly 
    770                 775                 780                 


Lys Thr Leu Asp Arg Trp Gln Ala Glu Lys Lys Pro Gln Ala Glu Pro 
785                 790                 795                 800 


Asp Arg Pro Met Ile Leu Ile Asp Asn Gln Glu Ser 
                805                 810         


<210>  232
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  232

Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210>  233
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  233
gaacacaaag catagactgc ggg                                               23


<210>  234
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  234
gtcatcttag tcattacctg agg                                               23


<210>  235
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  235
gagtccgagc agaagaagaa ggg                                               23


<210>  236
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  236
gctcccatca catcaaccgg tgg                                               23


<210>  237
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  237
ggcccagact gagcacgtga tgg                                               23


<210>  238
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  238
gtattactat tattatctga ga                                                22


<210>  239
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  239
gtgggactga tcccttaatg tg                                                22


<210>  240
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  240
gaccaggtca gcaaacatgt t                                                 21


<210>  241
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  241
gactcagcgc ccctgccggg cc                                                22


<210>  242
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  242
gccacagtgg gaggggacat g                                                 21


<210>  243
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  243
gcccagcaat tcactgtgaa g                                                 21


<210>  244
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  244
gcccagctcc agcctctgat g                                                 21


<210>  245
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  245
ggtcgaccct tggtatccat g                                                 21


<210>  246
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  246
ggtcgtagcc agtccgaacc c                                                 21


<210>  247
<211>  1129
<212>  PRT
<213>  Alicyclobacillus acido-terrestris


<220>
<221>  MISC_FEATURE
<222>  (1)..(1129)
<223>  Cas12b/C2c1; sp|T0D7A2|C2C1_ALIAG CRISPR-associated endonuclease 
       C2c1 OS = Alicyclobacillus acido-terrestris (strain ATCC 49025 / 
       DSM 3922/ CIP 106132 / NCIMB 13137/GD3B) GN=c2c1 PE=1 SV=1; 
       Cas12b/C2c1 polypeptide sequence

<400>  247

Met Ala Val Lys Ser Ile Lys Val Lys Leu Arg Leu Asp Asp Met Pro 
1               5                   10                  15      


Glu Ile Arg Ala Gly Leu Trp Lys Leu His Lys Glu Val Asn Ala Gly 
            20                  25                  30          


Val Arg Tyr Tyr Thr Glu Trp Leu Ser Leu Leu Arg Gln Glu Asn Leu 
        35                  40                  45              


Tyr Arg Arg Ser Pro Asn Gly Asp Gly Glu Gln Glu Cys Asp Lys Thr 
    50                  55                  60                  


Ala Glu Glu Cys Lys Ala Glu Leu Leu Glu Arg Leu Arg Ala Arg Gln 
65                  70                  75                  80  


Val Glu Asn Gly His Arg Gly Pro Ala Gly Ser Asp Asp Glu Leu Leu 
                85                  90                  95      


Gln Leu Ala Arg Gln Leu Tyr Glu Leu Leu Val Pro Gln Ala Ile Gly 
            100                 105                 110         


Ala Lys Gly Asp Ala Gln Gln Ile Ala Arg Lys Phe Leu Ser Pro Leu 
        115                 120                 125             


Ala Asp Lys Asp Ala Val Gly Gly Leu Gly Ile Ala Lys Ala Gly Asn 
    130                 135                 140                 


Lys Pro Arg Trp Val Arg Met Arg Glu Ala Gly Glu Pro Gly Trp Glu 
145                 150                 155                 160 


Glu Glu Lys Glu Lys Ala Glu Thr Arg Lys Ser Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Leu Arg Ala Leu Ala Asp Phe Gly Leu Lys Pro Leu Met Arg 
            180                 185                 190         


Val Tyr Thr Asp Ser Glu Met Ser Ser Val Glu Trp Lys Pro Leu Arg 
        195                 200                 205             


Lys Gly Gln Ala Val Arg Thr Trp Asp Arg Asp Met Phe Gln Gln Ala 
    210                 215                 220                 


Ile Glu Arg Met Met Ser Trp Glu Ser Trp Asn Gln Arg Val Gly Gln 
225                 230                 235                 240 


Glu Tyr Ala Lys Leu Val Glu Gln Lys Asn Arg Phe Glu Gln Lys Asn 
                245                 250                 255     


Phe Val Gly Gln Glu His Leu Val His Leu Val Asn Gln Leu Gln Gln 
            260                 265                 270         


Asp Met Lys Glu Ala Ser Pro Gly Leu Glu Ser Lys Glu Gln Thr Ala 
        275                 280                 285             


His Tyr Val Thr Gly Arg Ala Leu Arg Gly Ser Asp Lys Val Phe Glu 
    290                 295                 300                 


Lys Trp Gly Lys Leu Ala Pro Asp Ala Pro Phe Asp Leu Tyr Asp Ala 
305                 310                 315                 320 


Glu Ile Lys Asn Val Gln Arg Arg Asn Thr Arg Arg Phe Gly Ser His 
                325                 330                 335     


Asp Leu Phe Ala Lys Leu Ala Glu Pro Glu Tyr Gln Ala Leu Trp Arg 
            340                 345                 350         


Glu Asp Ala Ser Phe Leu Thr Arg Tyr Ala Val Tyr Asn Ser Ile Leu 
        355                 360                 365             


Arg Lys Leu Asn His Ala Lys Met Phe Ala Thr Phe Thr Leu Pro Asp 
    370                 375                 380                 


Ala Thr Ala His Pro Ile Trp Thr Arg Phe Asp Lys Leu Gly Gly Asn 
385                 390                 395                 400 


Leu His Gln Tyr Thr Phe Leu Phe Asn Glu Phe Gly Glu Arg Arg His 
                405                 410                 415     


Ala Ile Arg Phe His Lys Leu Leu Lys Val Glu Asn Gly Val Ala Arg 
            420                 425                 430         


Glu Val Asp Asp Val Thr Val Pro Ile Ser Met Ser Glu Gln Leu Asp 
        435                 440                 445             


Asn Leu Leu Pro Arg Asp Pro Asn Glu Pro Ile Ala Leu Tyr Phe Arg 
    450                 455                 460                 


Asp Tyr Gly Ala Glu Gln His Phe Thr Gly Glu Phe Gly Gly Ala Lys 
465                 470                 475                 480 


Ile Gln Cys Arg Arg Asp Gln Leu Ala His Met His Arg Arg Arg Gly 
                485                 490                 495     


Ala Arg Asp Val Tyr Leu Asn Val Ser Val Arg Val Gln Ser Gln Ser 
            500                 505                 510         


Glu Ala Arg Gly Glu Arg Arg Pro Pro Tyr Ala Ala Val Phe Arg Leu 
        515                 520                 525             


Val Gly Asp Asn His Arg Ala Phe Val His Phe Asp Lys Leu Ser Asp 
    530                 535                 540                 


Tyr Leu Ala Glu His Pro Asp Asp Gly Lys Leu Gly Ser Glu Gly Leu 
545                 550                 555                 560 


Leu Ser Gly Leu Arg Val Met Ser Val Asp Leu Gly Leu Arg Thr Ser 
                565                 570                 575     


Ala Ser Ile Ser Val Phe Arg Val Ala Arg Lys Asp Glu Leu Lys Pro 
            580                 585                 590         


Asn Ser Lys Gly Arg Val Pro Phe Phe Phe Pro Ile Lys Gly Asn Asp 
        595                 600                 605             


Asn Leu Val Ala Val His Glu Arg Ser Gln Leu Leu Lys Leu Pro Gly 
    610                 615                 620                 


Glu Thr Glu Ser Lys Asp Leu Arg Ala Ile Arg Glu Glu Arg Gln Arg 
625                 630                 635                 640 


Thr Leu Arg Gln Leu Arg Thr Gln Leu Ala Tyr Leu Arg Leu Leu Val 
                645                 650                 655     


Arg Cys Gly Ser Glu Asp Val Gly Arg Arg Glu Arg Ser Trp Ala Lys 
            660                 665                 670         


Leu Ile Glu Gln Pro Val Asp Ala Ala Asn His Met Thr Pro Asp Trp 
        675                 680                 685             


Arg Glu Ala Phe Glu Asn Glu Leu Gln Lys Leu Lys Ser Leu His Gly 
    690                 695                 700                 


Ile Cys Ser Asp Lys Glu Trp Met Asp Ala Val Tyr Glu Ser Val Arg 
705                 710                 715                 720 


Arg Val Trp Arg His Met Gly Lys Gln Val Arg Asp Trp Arg Lys Asp 
                725                 730                 735     


Val Arg Ser Gly Glu Arg Pro Lys Ile Arg Gly Tyr Ala Lys Asp Val 
            740                 745                 750         


Val Gly Gly Asn Ser Ile Glu Gln Ile Glu Tyr Leu Glu Arg Gln Tyr 
        755                 760                 765             


Lys Phe Leu Lys Ser Trp Ser Phe Phe Gly Lys Val Ser Gly Gln Val 
    770                 775                 780                 


Ile Arg Ala Glu Lys Gly Ser Arg Phe Ala Ile Thr Leu Arg Glu His 
785                 790                 795                 800 


Ile Asp His Ala Lys Glu Asp Arg Leu Lys Lys Leu Ala Asp Arg Ile 
                805                 810                 815     


Ile Met Glu Ala Leu Gly Tyr Val Tyr Ala Leu Asp Glu Arg Gly Lys 
            820                 825                 830         


Gly Lys Trp Val Ala Lys Tyr Pro Pro Cys Gln Leu Ile Leu Leu Glu 
        835                 840                 845             


Glu Leu Ser Glu Tyr Gln Phe Asn Asn Asp Arg Pro Pro Ser Glu Asn 
    850                 855                 860                 


Asn Gln Leu Met Gln Trp Ser His Arg Gly Val Phe Gln Glu Leu Ile 
865                 870                 875                 880 


Asn Gln Ala Gln Val His Asp Leu Leu Val Gly Thr Met Tyr Ala Ala 
                885                 890                 895     


Phe Ser Ser Arg Phe Asp Ala Arg Thr Gly Ala Pro Gly Ile Arg Cys 
            900                 905                 910         


Arg Arg Val Pro Ala Arg Cys Thr Gln Glu His Asn Pro Glu Pro Phe 
        915                 920                 925             


Pro Trp Trp Leu Asn Lys Phe Val Val Glu His Thr Leu Asp Ala Cys 
    930                 935                 940                 


Pro Leu Arg Ala Asp Asp Leu Ile Pro Thr Gly Glu Gly Glu Ile Phe 
945                 950                 955                 960 


Val Ser Pro Phe Ser Ala Glu Glu Gly Asp Phe His Gln Ile His Ala 
                965                 970                 975     


Asp Leu Asn Ala Ala Gln Asn Leu Gln Gln Arg Leu Trp Ser Asp Phe 
            980                 985                 990         


Asp Ile Ser Gln Ile Arg Leu Arg  Cys Asp Trp Gly Glu  Val Asp Gly 
        995                 1000                 1005             


Glu Leu  Val Leu Ile Pro Arg  Leu Thr Gly Lys Arg  Thr Ala Asp 
    1010                 1015                 1020             


Ser Tyr  Ser Asn Lys Val Phe  Tyr Thr Asn Thr Gly  Val Thr Tyr 
    1025                 1030                 1035             


Tyr Glu  Arg Glu Arg Gly Lys  Lys Arg Arg Lys Val  Phe Ala Gln 
    1040                 1045                 1050             


Glu Lys  Leu Ser Glu Glu Glu  Ala Glu Leu Leu Val  Glu Ala Asp 
    1055                 1060                 1065             


Glu Ala  Arg Glu Lys Ser Val  Val Leu Met Arg Asp  Pro Ser Gly 
    1070                 1075                 1080             


Ile Ile  Asn Arg Gly Asn Trp  Thr Arg Gln Lys Glu  Phe Trp Ser 
    1085                 1090                 1095             


Met Val  Asn Gln Arg Ile Glu  Gly Tyr Leu Val Lys  Gln Ile Arg 
    1100                 1105                 1110             


Ser Arg  Val Pro Leu Gln Asp  Ser Ala Cys Glu Asn  Thr Gly Asp 
    1115                 1120                 1125             


Ile 
    


<210>  248
<211>  1368
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1368)
<223>  high fidelity Cas9 polypeptide sequence

<400>  248

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Ala Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Ala Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Ala Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Ala Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  249
<211>  1368
<212>  PRT
<213>  S. pyogenes


<220>
<221>  MISC_FEATURE
<222>  (1)..(1368)
<223>  PAM-binding SpEQR Cas9 polypeptide sequence

<400>  249

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Val Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Glu  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Gln  Tyr Arg Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  250
<211>  1368
<212>  PRT
<213>  S. pyogenes


<220>
<221>  MISC_FEATURE
<222>  (1)..(1368)
<223>  PAM-binding SpVQR Cas9 polypeptide sequence

<400>  250

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Val  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Gln  Tyr Arg Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  251
<211>  1368
<212>  PRT
<213>  S. pyogenes


<220>
<221>  MISC_FEATURE
<222>  (1)..(1368)
<223>  SpVQR Cas9 polypeptide sequence

<400>  251

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Val  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Arg 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Glu  Tyr Arg Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  252
<211>  1359
<212>  PRT
<213>  Streptococcus macacae


<220>
<221>  MISC_FEATURE
<222>  (1)..(1359)
<223>  SpyMacCas9 polypeptide sequence

<400>  252

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Gly Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Ile Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Ala Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Gly Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 


His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Ile Gln  Thr Val Gly Gln Asn  Gly Gly Leu Phe Asp  Asp Asn Pro 
    1100                 1105                 1110             


Lys Ser  Pro Leu Glu Val Thr  Pro Ser Lys Leu Val  Pro Leu Lys 
    1115                 1120                 1125             


Lys Glu  Leu Asn Pro Lys Lys  Tyr Gly Gly Tyr Gln  Lys Pro Thr 
    1130                 1135                 1140             


Thr Ala  Tyr Pro Val Leu Leu  Ile Thr Asp Thr Lys  Gln Leu Ile 
    1145                 1150                 1155             


Pro Ile  Ser Val Met Asn Lys  Lys Gln Phe Glu Gln  Asn Pro Val 
    1160                 1165                 1170             


Lys Phe  Leu Arg Asp Arg Gly  Tyr Gln Gln Val Gly  Lys Asn Asp 
    1175                 1180                 1185             


Phe Ile  Lys Leu Pro Lys Tyr  Thr Leu Val Asp Ile  Gly Asp Gly 
    1190                 1195                 1200             


Ile Lys  Arg Leu Trp Ala Ser  Ser Lys Glu Ile His  Lys Gly Asn 
    1205                 1210                 1215             


Gln Leu  Val Val Ser Lys Lys  Ser Gln Ile Leu Leu  Tyr His Ala 
    1220                 1225                 1230             


His His  Leu Asp Ser Asp Leu  Ser Asn Asp Tyr Leu  Gln Asn His 
    1235                 1240                 1245             


Asn Gln  Gln Phe Asp Val Leu  Phe Asn Glu Ile Ile  Ser Phe Ser 
    1250                 1255                 1260             


Lys Lys  Cys Lys Leu Gly Lys  Glu His Ile Gln Lys  Ile Glu Asn 
    1265                 1270                 1275             


Val Tyr  Ser Asn Lys Lys Asn  Ser Ala Ser Ile Glu  Glu Leu Ala 
    1280                 1285                 1290             


Glu Ser  Phe Ile Lys Leu Leu  Gly Phe Thr Gln Leu  Gly Ala Thr 
    1295                 1300                 1305             


Ser Pro  Phe Asn Phe Leu Gly  Val Lys Leu Asn Gln  Lys Gln Tyr 
    1310                 1315                 1320             


Lys Gly  Lys Lys Asp Tyr Ile  Leu Pro Cys Thr Glu  Gly Thr Leu 
    1325                 1330                 1335             


Ile Arg  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Val Asp Leu 
    1340                 1345                 1350             


Ser Lys  Ile Gly Glu Asp 
    1355                 


<210>  253
<211>  1410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: CP5 polypeptide sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(341)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (342)..(362)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (363)..(1388)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1389)..(1410)
<223>  Bipartite nuclear localization sequence

<400>  253

Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met 
1               5                   10                  15      


Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys 
            20                  25                  30          


Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp 
        35                  40                  45              


Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln 
    50                  55                  60                  


Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys 
65                  70                  75                  80  


Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys 
                85                  90                  95      


Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Met Gln Pro Thr Val 
            100                 105                 110         


Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly Lys Ser Lys 
        115                 120                 125             


Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg 
    130                 135                 140                 


Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr 
145                 150                 155                 160 


Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu 
                165                 170                 175     


Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Lys Phe 
            180                 185                 190         


Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe 
        195                 200                 205             


Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp 
    210                 215                 220                 


Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu Asp 
225                 230                 235                 240 


Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala 
                245                 250                 255     


Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp 
            260                 265                 270         


Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu 
        275                 280                 285             


Thr Asn Leu Gly Ala Pro Arg Ala Phe Lys Tyr Phe Asp Thr Thr Ile 
    290                 295                 300                 


Ala Arg Lys Glu Tyr Arg Ser Thr Lys Glu Val Leu Asp Ala Thr Leu 
305                 310                 315                 320 


Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser 
                325                 330                 335     


Gln Leu Gly Gly Asp Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         


Ser Gly Gly Ser Gly Gly Ser Gly Gly Met Asp Lys Lys Tyr Ser Ile 
        355                 360                 365             


Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp 
    370                 375                 380                 


Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp 
385                 390                 395                 400 


Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser 
                405                 410                 415     


Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg 
            420                 425                 430         


Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser 
        435                 440                 445             


Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu 
    450                 455                 460                 


Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe 
465                 470                 475                 480 


Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile 
                485                 490                 495     


Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu 
            500                 505                 510         


Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His 
        515                 520                 525             


Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys 
    530                 535                 540                 


Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn 
545                 550                 555                 560 


Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg 
                565                 570                 575     


Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly 
            580                 585                 590         


Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly 
        595                 600                 605             


Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys 
    610                 615                 620                 


Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu 
625                 630                 635                 640 


Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn 
                645                 650                 655     


Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu 
            660                 665                 670         


Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu 
        675                 680                 685             


His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu 
    690                 695                 700                 


Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr 
705                 710                 715                 720 


Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe 
                725                 730                 735     


Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val 
            740                 745                 750         


Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn 
        755                 760                 765             


Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu 
    770                 775                 780                 


Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys 
785                 790                 795                 800 


Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
                805                 810                 815     


Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu 
            820                 825                 830         


Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser 
        835                 840                 845             


Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro 
    850                 855                 860                 


Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr 
865                 870                 875                 880 


Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg 
                885                 890                 895     


Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu 
            900                 905                 910         


Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp 
        915                 920                 925             


Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val 
    930                 935                 940                 


Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys 
945                 950                 955                 960 


Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile 
                965                 970                 975     


Leu Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met 
            980                 985                 990         


Ile Glu Glu Arg Leu Lys Thr Tyr  Ala His Leu Phe Asp  Asp Lys Val 
        995                 1000                 1005             


Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr Gly Trp  Gly Arg Leu 
    1010                 1015                 1020             


Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp Lys Gln  Ser Gly Lys 
    1025                 1030                 1035             


Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly Phe Ala  Asn Arg Asn 
    1040                 1045                 1050             


Phe Met  Gln Leu Ile His Asp  Asp Ser Leu Thr Phe  Lys Glu Asp 
    1055                 1060                 1065             


Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly Asp Ser  Leu His Glu 
    1070                 1075                 1080             


His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala Ile Lys  Lys Gly Ile 
    1085                 1090                 1095             


Leu Gln  Thr Val Lys Val Val  Asp Glu Leu Val Lys  Val Met Gly 
    1100                 1105                 1110             


Arg His  Lys Pro Glu Asn Ile  Val Ile Glu Met Ala  Arg Glu Asn 
    1115                 1120                 1125             


Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser Arg Glu  Arg Met Lys 
    1130                 1135                 1140             


Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly Ser Gln  Ile Leu Lys 
    1145                 1150                 1155             


Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn Glu  Lys Leu Tyr 
    1160                 1165                 1170             


Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  Asp Gln Glu 
    1175                 1180                 1185             


Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His Ile Val 
    1190                 1195                 1200             


Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val Leu 
    1205                 1210                 1215             


Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
    1220                 1225                 1230             


Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu 
    1235                 1240                 1245             


Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys 
    1250                 1255                 1260             


Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile 
    1265                 1270                 1275             


Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile Thr Lys  His Val Ala 
    1280                 1285                 1290             


Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp 
    1295                 1300                 1305             


Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu 
    1310                 1315                 1320             


Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe Tyr Lys  Val Arg Glu 
    1325                 1330                 1335             


Ile Asn  Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
    1340                 1345                 1350             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1355                 1360                 1365             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1370                 1375                 1380             


Ala Lys  Ser Glu Gln Glu Gly  Ala Asp Lys Arg Thr  Ala Asp Gly 
    1385                 1390                 1395             


Ser Glu  Phe Glu Ser Pro Lys  Lys Lys Arg Lys Val  
    1400                 1405                 1410 


<210>  254
<211>  1302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12c1 polypeptide sequence

<400>  254

Met Gln Thr Lys Lys Thr His Leu His Leu Ile Ser Ala Lys Ala Ser 
1               5                   10                  15      


Arg Lys Tyr Arg Arg Thr Ile Ala Cys Leu Ser Asp Thr Ala Lys Lys 
            20                  25                  30          


Asp Leu Glu Arg Arg Lys Gln Ser Gly Ala Ala Asp Pro Ala Gln Glu 
        35                  40                  45              


Leu Ser Cys Leu Lys Thr Ile Lys Phe Lys Leu Glu Val Pro Glu Gly 
    50                  55                  60                  


Ser Lys Leu Pro Ser Phe Asp Arg Ile Ser Gln Ile Tyr Asn Ala Leu 
65                  70                  75                  80  


Glu Thr Ile Glu Lys Gly Ser Leu Ser Tyr Leu Leu Phe Ala Leu Ile 
                85                  90                  95      


Leu Ser Gly Phe Arg Ile Phe Pro Asn Ser Ser Ala Ala Lys Thr Phe 
            100                 105                 110         


Ala Ser Ser Ser Cys Tyr Lys Asn Asp Gln Phe Ala Ser Gln Ile Lys 
        115                 120                 125             


Glu Ile Phe Gly Glu Met Val Lys Asn Phe Ile Pro Ser Glu Leu Glu 
    130                 135                 140                 


Ser Ile Leu Lys Lys Gly Arg Arg Lys Asn Asn Lys Asp Trp Thr Glu 
145                 150                 155                 160 


Glu Asn Ile Lys Arg Val Leu Asn Ser Glu Phe Gly Arg Lys Asn Ser 
                165                 170                 175     


Glu Gly Ser Ser Ala Leu Phe Asp Ser Phe Leu Ser Lys Phe Ser Gln 
            180                 185                 190         


Glu Leu Phe Arg Lys Phe Asp Ser Trp Asn Glu Val Asn Lys Lys Tyr 
        195                 200                 205             


Leu Glu Ala Ala Glu Leu Leu Asp Ser Met Leu Ala Ser Tyr Gly Pro 
    210                 215                 220                 


Phe Asp Ser Val Cys Lys Met Ile Gly Asp Ser Asp Ser Arg Asn Ser 
225                 230                 235                 240 


Leu Pro Asp Lys Ser Thr Ile Ala Phe Thr Asn Asn Ala Glu Ile Thr 
                245                 250                 255     


Val Asp Ile Glu Ser Ser Val Met Pro Tyr Met Ala Ile Ala Ala Leu 
            260                 265                 270         


Leu Arg Glu Tyr Arg Gln Ser Lys Ser Lys Ala Ala Pro Val Ala Tyr 
        275                 280                 285             


Val Gln Ser His Leu Thr Thr Thr Asn Gly Asn Gly Leu Ser Trp Phe 
    290                 295                 300                 


Phe Lys Phe Gly Leu Asp Leu Ile Arg Lys Ala Pro Val Ser Ser Lys 
305                 310                 315                 320 


Gln Ser Thr Ser Asp Gly Ser Lys Ser Leu Gln Glu Leu Phe Ser Val 
                325                 330                 335     


Pro Asp Asp Lys Leu Asp Gly Leu Lys Phe Ile Lys Glu Ala Cys Glu 
            340                 345                 350         


Ala Leu Pro Glu Ala Ser Leu Leu Cys Gly Glu Lys Gly Glu Leu Leu 
        355                 360                 365             


Gly Tyr Gln Asp Phe Arg Thr Ser Phe Ala Gly His Ile Asp Ser Trp 
    370                 375                 380                 


Val Ala Asn Tyr Val Asn Arg Leu Phe Glu Leu Ile Glu Leu Val Asn 
385                 390                 395                 400 


Gln Leu Pro Glu Ser Ile Lys Leu Pro Ser Ile Leu Thr Gln Lys Asn 
                405                 410                 415     


His Asn Leu Val Ala Ser Leu Gly Leu Gln Glu Ala Glu Val Ser His 
            420                 425                 430         


Ser Leu Glu Leu Phe Glu Gly Leu Val Lys Asn Val Arg Gln Thr Leu 
        435                 440                 445             


Lys Lys Leu Ala Gly Ile Asp Ile Ser Ser Ser Pro Asn Glu Gln Asp 
    450                 455                 460                 


Ile Lys Glu Phe Tyr Ala Phe Ser Asp Val Leu Asn Arg Leu Gly Ser 
465                 470                 475                 480 


Ile Arg Asn Gln Ile Glu Asn Ala Val Gln Thr Ala Lys Lys Asp Lys 
                485                 490                 495     


Ile Asp Leu Glu Ser Ala Ile Glu Trp Lys Glu Trp Lys Lys Leu Lys 
            500                 505                 510         


Lys Leu Pro Lys Leu Asn Gly Leu Gly Gly Gly Val Pro Lys Gln Gln 
        515                 520                 525             


Glu Leu Leu Asp Lys Ala Leu Glu Ser Val Lys Gln Ile Arg His Tyr 
    530                 535                 540                 


Gln Arg Ile Asp Phe Glu Arg Val Ile Gln Trp Ala Val Asn Glu His 
545                 550                 555                 560 


Cys Leu Glu Thr Val Pro Lys Phe Leu Val Asp Ala Glu Lys Lys Lys 
                565                 570                 575     


Ile Asn Lys Glu Ser Ser Thr Asp Phe Ala Ala Lys Glu Asn Ala Val 
            580                 585                 590         


Arg Phe Leu Leu Glu Gly Ile Gly Ala Ala Ala Arg Gly Lys Thr Asp 
        595                 600                 605             


Ser Val Ser Lys Ala Ala Tyr Asn Trp Phe Val Val Asn Asn Phe Leu 
    610                 615                 620                 


Ala Lys Lys Asp Leu Asn Arg Tyr Phe Ile Asn Cys Gln Gly Cys Ile 
625                 630                 635                 640 


Tyr Lys Pro Pro Tyr Ser Lys Arg Arg Ser Leu Ala Phe Ala Leu Arg 
                645                 650                 655     


Ser Asp Asn Lys Asp Thr Ile Glu Val Val Trp Glu Lys Phe Glu Thr 
            660                 665                 670         


Phe Tyr Lys Glu Ile Ser Lys Glu Ile Glu Lys Phe Asn Ile Phe Ser 
        675                 680                 685             


Gln Glu Phe Gln Thr Phe Leu His Leu Glu Asn Leu Arg Met Lys Leu 
    690                 695                 700                 


Leu Leu Arg Arg Ile Gln Lys Pro Ile Pro Ala Glu Ile Ala Phe Phe 
705                 710                 715                 720 


Ser Leu Pro Gln Glu Tyr Tyr Asp Ser Leu Pro Pro Asn Val Ala Phe 
                725                 730                 735     


Leu Ala Leu Asn Gln Glu Ile Thr Pro Ser Glu Tyr Ile Thr Gln Phe 
            740                 745                 750         


Asn Leu Tyr Ser Ser Phe Leu Asn Gly Asn Leu Ile Leu Leu Arg Arg 
        755                 760                 765             


Ser Arg Ser Tyr Leu Arg Ala Lys Phe Ser Trp Val Gly Asn Ser Lys 
    770                 775                 780                 


Leu Ile Tyr Ala Ala Lys Glu Ala Arg Leu Trp Lys Ile Pro Asn Ala 
785                 790                 795                 800 


Tyr Trp Lys Ser Asp Glu Trp Lys Met Ile Leu Asp Ser Asn Val Leu 
                805                 810                 815     


Val Phe Asp Lys Ala Gly Asn Val Leu Pro Ala Pro Thr Leu Lys Lys 
            820                 825                 830         


Val Cys Glu Arg Glu Gly Asp Leu Arg Leu Phe Tyr Pro Leu Leu Arg 
        835                 840                 845             


Gln Leu Pro His Asp Trp Cys Tyr Arg Asn Pro Phe Val Lys Ser Val 
    850                 855                 860                 


Gly Arg Glu Lys Asn Val Ile Glu Val Asn Lys Glu Gly Glu Pro Lys 
865                 870                 875                 880 


Val Ala Ser Ala Leu Pro Gly Ser Leu Phe Arg Leu Ile Gly Pro Ala 
                885                 890                 895     


Pro Phe Lys Ser Leu Leu Asp Asp Cys Phe Phe Asn Pro Leu Asp Lys 
            900                 905                 910         


Asp Leu Arg Glu Cys Met Leu Ile Val Asp Gln Glu Ile Ser Gln Lys 
        915                 920                 925             


Val Glu Ala Gln Lys Val Glu Ala Ser Leu Glu Ser Cys Thr Tyr Ser 
    930                 935                 940                 


Ile Ala Val Pro Ile Arg Tyr His Leu Glu Glu Pro Lys Val Ser Asn 
945                 950                 955                 960 


Gln Phe Glu Asn Val Leu Ala Ile Asp Gln Gly Glu Ala Gly Leu Ala 
                965                 970                 975     


Tyr Ala Val Phe Ser Leu Lys Ser Ile Gly Glu Ala Glu Thr Lys Pro 
            980                 985                 990         


Ile Ala Val Gly Thr Ile Arg Ile  Pro Ser Ile Arg Arg  Leu Ile His 
        995                 1000                 1005             


Ser Val  Ser Thr Tyr Arg Lys  Lys Lys Gln Arg Leu  Gln Asn Phe 
    1010                 1015                 1020             


Lys Gln  Asn Tyr Asp Ser Thr  Ala Phe Ile Met Arg  Glu Asn Val 
    1025                 1030                 1035             


Thr Gly  Asp Val Cys Ala Lys  Ile Val Gly Leu Met  Lys Glu Phe 
    1040                 1045                 1050             


Asn Ala  Phe Pro Val Leu Glu  Tyr Asp Val Lys Asn  Leu Glu Ser 
    1055                 1060                 1065             


Gly Ser  Arg Gln Leu Ser Ala  Val Tyr Lys Ala Val  Asn Ser His 
    1070                 1075                 1080             


Phe Leu  Tyr Phe Lys Glu Pro  Gly Arg Asp Ala Leu  Arg Lys Gln 
    1085                 1090                 1095             


Leu Trp  Tyr Gly Gly Asp Ser  Trp Thr Ile Asp Gly  Ile Glu Ile 
    1100                 1105                 1110             


Val Thr  Arg Glu Arg Lys Glu  Asp Gly Lys Glu Gly  Val Glu Lys 
    1115                 1120                 1125             


Ile Val  Pro Leu Lys Val Phe  Pro Gly Arg Ser Val  Ser Ala Arg 
    1130                 1135                 1140             


Phe Thr  Ser Lys Thr Cys Ser  Cys Cys Gly Arg Asn  Val Phe Asp 
    1145                 1150                 1155             


Trp Leu  Phe Thr Glu Lys Lys  Ala Lys Thr Asn Lys  Lys Phe Asn 
    1160                 1165                 1170             


Val Asn  Ser Lys Gly Glu Leu  Thr Thr Ala Asp Gly  Val Ile Gln 
    1175                 1180                 1185             


Leu Phe  Glu Ala Asp Arg Ser  Lys Gly Pro Lys Phe  Tyr Ala Arg 
    1190                 1195                 1200             


Arg Lys  Glu Arg Thr Pro Leu  Thr Lys Pro Ile Ala  Lys Gly Ser 
    1205                 1210                 1215             


Tyr Ser  Leu Glu Glu Ile Glu  Arg Arg Val Arg Thr  Asn Leu Arg 
    1220                 1225                 1230             


Arg Ala  Pro Lys Ser Lys Gln  Ser Arg Asp Thr Ser  Gln Ser Gln 
    1235                 1240                 1245             


Tyr Phe  Cys Val Tyr Lys Asp  Cys Ala Leu His Phe  Ser Gly Met 
    1250                 1255                 1260             


Gln Ala  Asp Glu Asn Ala Ala  Ile Asn Ile Gly Arg  Arg Phe Leu 
    1265                 1270                 1275             


Thr Ala  Leu Arg Lys Asn Arg  Arg Ser Asp Phe Pro  Ser Asn Val 
    1280                 1285                 1290             


Lys Ile  Ser Asp Arg Leu Leu  Asp Asn 
    1295                 1300         


<210>  255
<211>  1218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12c2 polypeptide sequence

<400>  255

Met Thr Lys His Ser Ile Pro Leu His Ala Phe Arg Asn Ser Gly Ala 
1               5                   10                  15      


Asp Ala Arg Lys Trp Lys Gly Arg Ile Ala Leu Leu Ala Lys Arg Gly 
            20                  25                  30          


Lys Glu Thr Met Arg Thr Leu Gln Phe Pro Leu Glu Met Ser Glu Pro 
        35                  40                  45              


Glu Ala Ala Ala Ile Asn Thr Thr Pro Phe Ala Val Ala Tyr Asn Ala 
    50                  55                  60                  


Ile Glu Gly Thr Gly Lys Gly Thr Leu Phe Asp Tyr Trp Ala Lys Leu 
65                  70                  75                  80  


His Leu Ala Gly Phe Arg Phe Phe Pro Ser Gly Gly Ala Ala Thr Ile 
                85                  90                  95      


Phe Arg Gln Gln Ala Val Phe Glu Asp Ala Ser Trp Asn Ala Ala Phe 
            100                 105                 110         


Cys Gln Gln Ser Gly Lys Asp Trp Pro Trp Leu Val Pro Ser Lys Leu 
        115                 120                 125             


Tyr Glu Arg Phe Thr Lys Ala Pro Arg Glu Val Ala Lys Lys Asp Gly 
    130                 135                 140                 


Ser Lys Lys Ser Ile Glu Phe Thr Gln Glu Asn Val Ala Asn Glu Ser 
145                 150                 155                 160 


His Val Ser Leu Val Gly Ala Ser Ile Thr Asp Lys Thr Pro Glu Asp 
                165                 170                 175     


Gln Lys Glu Phe Phe Leu Lys Met Ala Gly Ala Leu Ala Glu Lys Phe 
            180                 185                 190         


Asp Ser Trp Lys Ser Ala Asn Glu Asp Arg Ile Val Ala Met Lys Val 
        195                 200                 205             


Ile Asp Glu Phe Leu Lys Ser Glu Gly Leu His Leu Pro Ser Leu Glu 
    210                 215                 220                 


Asn Ile Ala Val Lys Cys Ser Val Glu Thr Lys Pro Asp Asn Ala Thr 
225                 230                 235                 240 


Val Ala Trp His Asp Ala Pro Met Ser Gly Val Gln Asn Leu Ala Ile 
                245                 250                 255     


Gly Val Phe Ala Thr Cys Ala Ser Arg Ile Asp Asn Ile Tyr Asp Leu 
            260                 265                 270         


Asn Gly Gly Lys Leu Ser Lys Leu Ile Gln Glu Ser Ala Thr Thr Pro 
        275                 280                 285             


Asn Val Thr Ala Leu Ser Trp Leu Phe Gly Lys Gly Leu Glu Tyr Phe 
    290                 295                 300                 


Arg Thr Thr Asp Ile Asp Thr Ile Met Gln Asp Phe Asn Ile Pro Ala 
305                 310                 315                 320 


Ser Ala Lys Glu Ser Ile Lys Pro Leu Val Glu Ser Ala Gln Ala Ile 
                325                 330                 335     


Pro Thr Met Thr Val Leu Gly Lys Lys Asn Tyr Ala Pro Phe Arg Pro 
            340                 345                 350         


Asn Phe Gly Gly Lys Ile Asp Ser Trp Ile Ala Asn Tyr Ala Ser Arg 
        355                 360                 365             


Leu Met Leu Leu Asn Asp Ile Leu Glu Gln Ile Glu Pro Gly Phe Glu 
    370                 375                 380                 


Leu Pro Gln Ala Leu Leu Asp Asn Glu Thr Leu Met Ser Gly Ile Asp 
385                 390                 395                 400 


Met Thr Gly Asp Glu Leu Lys Glu Leu Ile Glu Ala Val Tyr Ala Trp 
                405                 410                 415     


Val Asp Ala Ala Lys Gln Gly Leu Ala Thr Leu Leu Gly Arg Gly Gly 
            420                 425                 430         


Asn Val Asp Asp Ala Val Gln Thr Phe Glu Gln Phe Ser Ala Met Met 
        435                 440                 445             


Asp Thr Leu Asn Gly Thr Leu Asn Thr Ile Ser Ala Arg Tyr Val Arg 
    450                 455                 460                 


Ala Val Glu Met Ala Gly Lys Asp Glu Ala Arg Leu Glu Lys Leu Ile 
465                 470                 475                 480 


Glu Cys Lys Phe Asp Ile Pro Lys Trp Cys Lys Ser Val Pro Lys Leu 
                485                 490                 495     


Val Gly Ile Ser Gly Gly Leu Pro Lys Val Glu Glu Glu Ile Lys Val 
            500                 505                 510         


Met Asn Ala Ala Phe Lys Asp Val Arg Ala Arg Met Phe Val Arg Phe 
        515                 520                 525             


Glu Glu Ile Ala Ala Tyr Val Ala Ser Lys Gly Ala Gly Met Asp Val 
    530                 535                 540                 


Tyr Asp Ala Leu Glu Lys Arg Glu Leu Glu Gln Ile Lys Lys Leu Lys 
545                 550                 555                 560 


Ser Ala Val Pro Glu Arg Ala His Ile Gln Ala Tyr Arg Ala Val Leu 
                565                 570                 575     


His Arg Ile Gly Arg Ala Val Gln Asn Cys Ser Glu Lys Thr Lys Gln 
            580                 585                 590         


Leu Phe Ser Ser Lys Val Ile Glu Met Gly Val Phe Lys Asn Pro Ser 
        595                 600                 605             


His Leu Asn Asn Phe Ile Phe Asn Gln Lys Gly Ala Ile Tyr Arg Ser 
    610                 615                 620                 


Pro Phe Asp Arg Ser Arg His Ala Pro Tyr Gln Leu His Ala Asp Lys 
625                 630                 635                 640 


Leu Leu Lys Asn Asp Trp Leu Glu Leu Leu Ala Glu Ile Ser Ala Thr 
                645                 650                 655     


Leu Met Ala Ser Glu Ser Thr Glu Gln Met Glu Asp Ala Leu Arg Leu 
            660                 665                 670         


Glu Arg Thr Arg Leu Gln Leu Gln Leu Ser Gly Leu Pro Asp Trp Glu 
        675                 680                 685             


Tyr Pro Ala Ser Leu Ala Lys Pro Asp Ile Glu Val Glu Ile Gln Thr 
    690                 695                 700                 


Ala Leu Lys Met Gln Leu Ala Lys Asp Thr Val Thr Ser Asp Val Leu 
705                 710                 715                 720 


Gln Arg Ala Phe Asn Leu Tyr Ser Ser Val Leu Ser Gly Leu Thr Phe 
                725                 730                 735     


Lys Leu Leu Arg Arg Ser Phe Ser Leu Lys Met Arg Phe Ser Val Ala 
            740                 745                 750         


Asp Thr Thr Gln Leu Ile Tyr Val Pro Lys Val Cys Asp Trp Ala Ile 
        755                 760                 765             


Pro Lys Gln Tyr Leu Gln Ala Glu Gly Glu Ile Gly Ile Ala Ala Arg 
    770                 775                 780                 


Val Val Thr Glu Ser Ser Pro Ala Lys Met Val Thr Glu Val Glu Met 
785                 790                 795                 800 


Lys Glu Pro Lys Ala Leu Gly His Phe Met Gln Gln Ala Pro His Asp 
                805                 810                 815     


Trp Tyr Phe Asp Ala Ser Leu Gly Gly Thr Gln Val Ala Gly Arg Ile 
            820                 825                 830         


Val Glu Lys Gly Lys Glu Val Gly Lys Glu Arg Lys Leu Val Gly Tyr 
        835                 840                 845             


Arg Met Arg Gly Asn Ser Ala Tyr Lys Thr Val Leu Asp Lys Ser Leu 
    850                 855                 860                 


Val Gly Asn Thr Glu Leu Ser Gln Cys Ser Met Ile Ile Glu Ile Pro 
865                 870                 875                 880 


Tyr Thr Gln Thr Val Asp Ala Asp Phe Arg Ala Gln Val Gln Ala Gly 
                885                 890                 895     


Leu Pro Lys Val Ser Ile Asn Leu Pro Val Lys Glu Thr Ile Thr Ala 
            900                 905                 910         


Ser Asn Lys Asp Glu Gln Met Leu Phe Asp Arg Phe Val Ala Ile Asp 
        915                 920                 925             


Leu Gly Glu Arg Gly Leu Gly Tyr Ala Val Phe Asp Ala Lys Thr Leu 
    930                 935                 940                 


Glu Leu Gln Glu Ser Gly His Arg Pro Ile Lys Ala Ile Thr Asn Leu 
945                 950                 955                 960 


Leu Asn Arg Thr His His Tyr Glu Gln Arg Pro Asn Gln Arg Gln Lys 
                965                 970                 975     


Phe Gln Ala Lys Phe Asn Val Asn Leu Ser Glu Leu Arg Glu Asn Thr 
            980                 985                 990         


Val Gly Asp Val Cys His Gln Ile  Asn Arg Ile Cys Ala  Tyr Tyr Asn 
        995                 1000                 1005             


Ala Phe  Pro Val Leu Glu Tyr  Met Val Pro Asp Arg  Leu Asp Lys 
    1010                 1015                 1020             


Gln Leu  Lys Ser Val Tyr Glu  Ser Val Thr Asn Arg  Tyr Ile Trp 
    1025                 1030                 1035             


Ser Ser  Thr Asp Ala His Lys  Ser Ala Arg Val Gln  Phe Trp Leu 
    1040                 1045                 1050             


Gly Gly  Glu Thr Trp Glu His  Pro Tyr Leu Lys Ser  Ala Lys Asp 
    1055                 1060                 1065             


Lys Lys  Pro Leu Val Leu Ser  Pro Gly Arg Gly Ala  Ser Gly Lys 
    1070                 1075                 1080             


Gly Thr  Ser Gln Thr Cys Ser  Cys Cys Gly Arg Asn  Pro Phe Asp 
    1085                 1090                 1095             


Leu Ile  Lys Asp Met Lys Pro  Arg Ala Lys Ile Ala  Val Val Asp 
    1100                 1105                 1110             


Gly Lys  Ala Lys Leu Glu Asn  Ser Glu Leu Lys Leu  Phe Glu Arg 
    1115                 1120                 1125             


Asn Leu  Glu Ser Lys Asp Asp  Met Leu Ala Arg Arg  His Arg Asn 
    1130                 1135                 1140             


Glu Arg  Ala Gly Met Glu Gln  Pro Leu Thr Pro Gly  Asn Tyr Thr 
    1145                 1150                 1155             


Val Asp  Glu Ile Lys Ala Leu  Leu Arg Ala Asn Leu  Arg Arg Ala 
    1160                 1165                 1170             


Pro Lys  Asn Arg Arg Thr Lys  Asp Thr Thr Val Ser  Glu Tyr His 
    1175                 1180                 1185             


Cys Val  Phe Ser Asp Cys Gly  Lys Thr Met His Ala  Asp Glu Asn 
    1190                 1195                 1200             


Ala Ala  Val Asn Ile Gly Gly  Lys Phe Ile Ala Asp  Ile Glu Lys 
    1205                 1210                 1215             


<210>  256
<211>  1252
<212>  PRT
<213>  Oleiphilus sp. HI0009


<220>
<221>  MISC_FEATURE
<222>  (1)..(1252)
<223>  OspCas12c polypeptide sequence

<400>  256

Met Thr Lys Leu Arg His Arg Gln Lys Lys Leu Thr His Asp Trp Ala 
1               5                   10                  15      


Gly Ser Lys Lys Arg Glu Val Leu Gly Ser Asn Gly Lys Leu Gln Asn 
            20                  25                  30          


Pro Leu Leu Met Pro Val Lys Lys Gly Gln Val Thr Glu Phe Arg Lys 
        35                  40                  45              


Ala Phe Ser Ala Tyr Ala Arg Ala Thr Lys Gly Glu Met Thr Asp Gly 
    50                  55                  60                  


Arg Lys Asn Met Phe Thr His Ser Phe Glu Pro Phe Lys Thr Lys Pro 
65                  70                  75                  80  


Ser Leu His Gln Cys Glu Leu Ala Asp Lys Ala Tyr Gln Ser Leu His 
                85                  90                  95      


Ser Tyr Leu Pro Gly Ser Leu Ala His Phe Leu Leu Ser Ala His Ala 
            100                 105                 110         


Leu Gly Phe Arg Ile Phe Ser Lys Ser Gly Glu Ala Thr Ala Phe Gln 
        115                 120                 125             


Ala Ser Ser Lys Ile Glu Ala Tyr Glu Ser Lys Leu Ala Ser Glu Leu 
    130                 135                 140                 


Ala Cys Val Asp Leu Ser Ile Gln Asn Leu Thr Ile Ser Thr Leu Phe 
145                 150                 155                 160 


Asn Ala Leu Thr Thr Ser Val Arg Gly Lys Gly Glu Glu Thr Ser Ala 
                165                 170                 175     


Asp Pro Leu Ile Ala Arg Phe Tyr Thr Leu Leu Thr Gly Lys Pro Leu 
            180                 185                 190         


Ser Arg Asp Thr Gln Gly Pro Glu Arg Asp Leu Ala Glu Val Ile Ser 
        195                 200                 205             


Arg Lys Ile Ala Ser Ser Phe Gly Thr Trp Lys Glu Met Thr Ala Asn 
    210                 215                 220                 


Pro Leu Gln Ser Leu Gln Phe Phe Glu Glu Glu Leu His Ala Leu Asp 
225                 230                 235                 240 


Ala Asn Val Ser Leu Ser Pro Ala Phe Asp Val Leu Ile Lys Met Asn 
                245                 250                 255     


Asp Leu Gln Gly Asp Leu Lys Asn Arg Thr Ile Val Phe Asp Pro Asp 
            260                 265                 270         


Ala Pro Val Phe Glu Tyr Asn Ala Glu Asp Pro Ala Asp Ile Ile Ile 
        275                 280                 285             


Lys Leu Thr Ala Arg Tyr Ala Lys Glu Ala Val Ile Lys Asn Gln Asn 
    290                 295                 300                 


Val Gly Asn Tyr Val Lys Asn Ala Ile Thr Thr Thr Asn Ala Asn Gly 
305                 310                 315                 320 


Leu Gly Trp Leu Leu Asn Lys Gly Leu Ser Leu Leu Pro Val Ser Thr 
                325                 330                 335     


Asp Asp Glu Leu Leu Glu Phe Ile Gly Val Glu Arg Ser His Pro Ser 
            340                 345                 350         


Cys His Ala Leu Ile Glu Leu Ile Ala Gln Leu Glu Ala Pro Glu Leu 
        355                 360                 365             


Phe Glu Lys Asn Val Phe Ser Asp Thr Arg Ser Glu Val Gln Gly Met 
    370                 375                 380                 


Ile Asp Ser Ala Val Ser Asn His Ile Ala Arg Leu Ser Ser Ser Arg 
385                 390                 395                 400 


Asn Ser Leu Ser Met Asp Ser Glu Glu Leu Glu Arg Leu Ile Lys Ser 
                405                 410                 415     


Phe Gln Ile His Thr Pro His Cys Ser Leu Phe Ile Gly Ala Gln Ser 
            420                 425                 430         


Leu Ser Gln Gln Leu Glu Ser Leu Pro Glu Ala Leu Gln Ser Gly Val 
        435                 440                 445             


Asn Ser Ala Asp Ile Leu Leu Gly Ser Thr Gln Tyr Met Leu Thr Asn 
    450                 455                 460                 


Ser Leu Val Glu Glu Ser Ile Ala Thr Tyr Gln Arg Thr Leu Asn Arg 
465                 470                 475                 480 


Ile Asn Tyr Leu Ser Gly Val Ala Gly Gln Ile Asn Gly Ala Ile Lys 
                485                 490                 495     


Arg Lys Ala Ile Asp Gly Glu Lys Ile His Leu Pro Ala Ala Trp Ser 
            500                 505                 510         


Glu Leu Ile Ser Leu Pro Phe Ile Gly Gln Pro Val Ile Asp Val Glu 
        515                 520                 525             


Ser Asp Leu Ala His Leu Lys Asn Gln Tyr Gln Thr Leu Ser Asn Glu 
    530                 535                 540                 


Phe Asp Thr Leu Ile Ser Ala Leu Gln Lys Asn Phe Asp Leu Asn Phe 
545                 550                 555                 560 


Asn Lys Ala Leu Leu Asn Arg Thr Gln His Phe Glu Ala Met Cys Arg 
                565                 570                 575     


Ser Thr Lys Lys Asn Ala Leu Ser Lys Pro Glu Ile Val Ser Tyr Arg 
            580                 585                 590         


Asp Leu Leu Ala Arg Leu Thr Ser Cys Leu Tyr Arg Gly Ser Leu Val 
        595                 600                 605             


Leu Arg Arg Ala Gly Ile Glu Val Leu Lys Lys His Lys Ile Phe Glu 
    610                 615                 620                 


Ser Asn Ser Glu Leu Arg Glu His Val His Glu Arg Lys His Phe Val 
625                 630                 635                 640 


Phe Val Ser Pro Leu Asp Arg Lys Ala Lys Lys Leu Leu Arg Leu Thr 
                645                 650                 655     


Asp Ser Arg Pro Asp Leu Leu His Val Ile Asp Glu Ile Leu Gln His 
            660                 665                 670         


Asp Asn Leu Glu Asn Lys Asp Arg Glu Ser Leu Trp Leu Val Arg Ser 
        675                 680                 685             


Gly Tyr Leu Leu Ala Gly Leu Pro Asp Gln Leu Ser Ser Ser Phe Ile 
    690                 695                 700                 


Asn Leu Pro Ile Ile Thr Gln Lys Gly Asp Arg Arg Leu Ile Asp Leu 
705                 710                 715                 720 


Ile Gln Tyr Asp Gln Ile Asn Arg Asp Ala Phe Val Met Leu Val Thr 
                725                 730                 735     


Ser Ala Phe Lys Ser Asn Leu Ser Gly Leu Gln Tyr Arg Ala Asn Lys 
            740                 745                 750         


Gln Ser Phe Val Val Thr Arg Thr Leu Ser Pro Tyr Leu Gly Ser Lys 
        755                 760                 765             


Leu Val Tyr Val Pro Lys Asp Lys Asp Trp Leu Val Pro Ser Gln Met 
    770                 775                 780                 


Phe Glu Gly Arg Phe Ala Asp Ile Leu Gln Ser Asp Tyr Met Val Trp 
785                 790                 795                 800 


Lys Asp Ala Gly Arg Leu Cys Val Ile Asp Thr Ala Lys His Leu Ser 
                805                 810                 815     


Asn Ile Lys Lys Ser Val Phe Ser Ser Glu Glu Val Leu Ala Phe Leu 
            820                 825                 830         


Arg Glu Leu Pro His Arg Thr Phe Ile Gln Thr Glu Val Arg Gly Leu 
        835                 840                 845             


Gly Val Asn Val Asp Gly Ile Ala Phe Asn Asn Gly Asp Ile Pro Ser 
    850                 855                 860                 


Leu Lys Thr Phe Ser Asn Cys Val Gln Val Lys Val Ser Arg Thr Asn 
865                 870                 875                 880 


Thr Ser Leu Val Gln Thr Leu Asn Arg Trp Phe Glu Gly Gly Lys Val 
                885                 890                 895     


Ser Pro Pro Ser Ile Gln Phe Glu Arg Ala Tyr Tyr Lys Lys Asp Asp 
            900                 905                 910         


Gln Ile His Glu Asp Ala Ala Lys Arg Lys Ile Arg Phe Gln Met Pro 
        915                 920                 925             


Ala Thr Glu Leu Val His Ala Ser Asp Asp Ala Gly Trp Thr Pro Ser 
    930                 935                 940                 


Tyr Leu Leu Gly Ile Asp Pro Gly Glu Tyr Gly Met Gly Leu Ser Leu 
945                 950                 955                 960 


Val Ser Ile Asn Asn Gly Glu Val Leu Asp Ser Gly Phe Ile His Ile 
                965                 970                 975     


Asn Ser Leu Ile Asn Phe Ala Ser Lys Lys Ser Asn His Gln Thr Lys 
            980                 985                 990         


Val Val Pro Arg Gln Gln Tyr Lys  Ser Pro Tyr Ala Asn  Tyr Leu Glu 
        995                 1000                 1005             


Gln Ser  Lys Asp Ser Ala Ala  Gly Asp Ile Ala His  Ile Leu Asp 
    1010                 1015                 1020             


Arg Leu  Ile Tyr Lys Leu Asn  Ala Leu Pro Val Phe  Glu Ala Leu 
    1025                 1030                 1035             


Ser Gly  Asn Ser Gln Ser Ala  Ala Asp Gln Val Trp  Thr Lys Val 
    1040                 1045                 1050             


Leu Ser  Phe Tyr Thr Trp Gly  Asp Asn Asp Ala Gln  Asn Ser Ile 
    1055                 1060                 1065             


Arg Lys  Gln His Trp Phe Gly  Ala Ser His Trp Asp  Ile Lys Gly 
    1070                 1075                 1080             


Met Leu  Arg Gln Pro Pro Thr  Glu Lys Lys Pro Lys  Pro Tyr Ile 
    1085                 1090                 1095             


Ala Phe  Pro Gly Ser Gln Val  Ser Ser Tyr Gly Asn  Ser Gln Arg 
    1100                 1105                 1110             


Cys Ser  Cys Cys Gly Arg Asn  Pro Ile Glu Gln Leu  Arg Glu Met 
    1115                 1120                 1125             


Ala Lys  Asp Thr Ser Ile Lys  Glu Leu Lys Ile Arg  Asn Ser Glu 
    1130                 1135                 1140             


Ile Gln  Leu Phe Asp Gly Thr  Ile Lys Leu Phe Asn  Pro Asp Pro 
    1145                 1150                 1155             


Ser Thr  Val Ile Glu Arg Arg  Arg His Asn Leu Gly  Pro Ser Arg 
    1160                 1165                 1170             


Ile Pro  Val Ala Asp Arg Thr  Phe Lys Asn Ile Ser  Pro Ser Ser 
    1175                 1180                 1185             


Leu Glu  Phe Lys Glu Leu Ile  Thr Ile Val Ser Arg  Ser Ile Arg 
    1190                 1195                 1200             


His Ser  Pro Glu Phe Ile Ala  Lys Lys Arg Gly Ile  Gly Ser Glu 
    1205                 1210                 1215             


Tyr Phe  Cys Ala Tyr Ser Asp  Cys Asn Ser Ser Leu  Asn Ser Glu 
    1220                 1225                 1230             


Ala Asn  Ala Ala Ala Asn Val  Ala Gln Lys Phe Gln  Lys Gln Leu 
    1235                 1240                 1245             


Phe Phe  Glu Leu 
    1250         


<210>  257
<211>  767
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12g1 polypeptide sequence

<400>  257

Met Ala Gln Ala Ser Ser Thr Pro Ala Val Ser Pro Arg Pro Arg Pro 
1               5                   10                  15      


Arg Tyr Arg Glu Glu Arg Thr Leu Val Arg Lys Leu Leu Pro Arg Pro 
            20                  25                  30          


Gly Gln Ser Lys Gln Glu Phe Arg Glu Asn Val Lys Lys Leu Arg Lys 
        35                  40                  45              


Ala Phe Leu Gln Phe Asn Ala Asp Val Ser Gly Val Cys Gln Trp Ala 
    50                  55                  60                  


Ile Gln Phe Arg Pro Arg Tyr Gly Lys Pro Ala Glu Pro Thr Glu Thr 
65                  70                  75                  80  


Phe Trp Lys Phe Phe Leu Glu Pro Glu Thr Ser Leu Pro Pro Asn Asp 
                85                  90                  95      


Ser Arg Ser Pro Glu Phe Arg Arg Leu Gln Ala Phe Glu Ala Ala Ala 
            100                 105                 110         


Gly Ile Asn Gly Ala Ala Ala Leu Asp Asp Pro Ala Phe Thr Asn Glu 
        115                 120                 125             


Leu Arg Asp Ser Ile Leu Ala Val Ala Ser Arg Pro Lys Thr Lys Glu 
    130                 135                 140                 


Ala Gln Arg Leu Phe Ser Arg Leu Lys Asp Tyr Gln Pro Ala His Arg 
145                 150                 155                 160 


Met Ile Leu Ala Lys Val Ala Ala Glu Trp Ile Glu Ser Arg Tyr Arg 
                165                 170                 175     


Arg Ala His Gln Asn Trp Glu Arg Asn Tyr Glu Glu Trp Lys Lys Glu 
            180                 185                 190         


Lys Gln Glu Trp Glu Gln Asn His Pro Glu Leu Thr Pro Glu Ile Arg 
        195                 200                 205             


Glu Ala Phe Asn Gln Ile Phe Gln Gln Leu Glu Val Lys Glu Lys Arg 
    210                 215                 220                 


Val Arg Ile Cys Pro Ala Ala Arg Leu Leu Gln Asn Lys Asp Asn Cys 
225                 230                 235                 240 


Gln Tyr Ala Gly Lys Asn Lys His Ser Val Leu Cys Asn Gln Phe Asn 
                245                 250                 255     


Glu Phe Lys Lys Asn His Leu Gln Gly Lys Ala Ile Lys Phe Phe Tyr 
            260                 265                 270         


Lys Asp Ala Glu Lys Tyr Leu Arg Cys Gly Leu Gln Ser Leu Lys Pro 
        275                 280                 285             


Asn Val Gln Gly Pro Phe Arg Glu Asp Trp Asn Lys Tyr Leu Arg Tyr 
    290                 295                 300                 


Met Asn Leu Lys Glu Glu Thr Leu Arg Gly Lys Asn Gly Gly Arg Leu 
305                 310                 315                 320 


Pro His Cys Lys Asn Leu Gly Gln Glu Cys Glu Phe Asn Pro His Thr 
                325                 330                 335     


Ala Leu Cys Lys Gln Tyr Gln Gln Gln Leu Ser Ser Arg Pro Asp Leu 
            340                 345                 350         


Val Gln His Asp Glu Leu Tyr Arg Lys Trp Arg Arg Glu Tyr Trp Arg 
        355                 360                 365             


Glu Pro Arg Lys Pro Val Phe Arg Tyr Pro Ser Val Lys Arg His Ser 
    370                 375                 380                 


Ile Ala Lys Ile Phe Gly Glu Asn Tyr Phe Gln Ala Asp Phe Lys Asn 
385                 390                 395                 400 


Ser Val Val Gly Leu Arg Leu Asp Ser Met Pro Ala Gly Gln Tyr Leu 
                405                 410                 415     


Glu Phe Ala Phe Ala Pro Trp Pro Arg Asn Tyr Arg Pro Gln Pro Gly 
            420                 425                 430         


Glu Thr Glu Ile Ser Ser Val His Leu His Phe Val Gly Thr Arg Pro 
        435                 440                 445             


Arg Ile Gly Phe Arg Phe Arg Val Pro His Lys Arg Ser Arg Phe Asp 
    450                 455                 460                 


Cys Thr Gln Glu Glu Leu Asp Glu Leu Arg Ser Arg Thr Phe Pro Arg 
465                 470                 475                 480 


Lys Ala Gln Asp Gln Lys Phe Leu Glu Ala Ala Arg Lys Arg Leu Leu 
                485                 490                 495     


Glu Thr Phe Pro Gly Asn Ala Glu Gln Glu Leu Arg Leu Leu Ala Val 
            500                 505                 510         


Asp Leu Gly Thr Asp Ser Ala Arg Ala Ala Phe Phe Ile Gly Lys Thr 
        515                 520                 525             


Phe Gln Gln Ala Phe Pro Leu Lys Ile Val Lys Ile Glu Lys Leu Tyr 
    530                 535                 540                 


Glu Gln Trp Pro Asn Gln Lys Gln Ala Gly Asp Arg Arg Asp Ala Ser 
545                 550                 555                 560 


Ser Lys Gln Pro Arg Pro Gly Leu Ser Arg Asp His Val Gly Arg His 
                565                 570                 575     


Leu Gln Lys Met Arg Ala Gln Ala Ser Glu Ile Ala Gln Lys Arg Gln 
            580                 585                 590         


Glu Leu Thr Gly Thr Pro Ala Pro Glu Thr Thr Thr Asp Gln Ala Ala 
        595                 600                 605             


Lys Lys Ala Thr Leu Gln Pro Phe Asp Leu Arg Gly Leu Thr Val His 
    610                 615                 620                 


Thr Ala Arg Met Ile Arg Asp Trp Ala Arg Leu Asn Ala Arg Gln Ile 
625                 630                 635                 640 


Ile Gln Leu Ala Glu Glu Asn Gln Val Asp Leu Ile Val Leu Glu Ser 
                645                 650                 655     


Leu Arg Gly Phe Arg Pro Pro Gly Tyr Glu Asn Leu Asp Gln Glu Lys 
            660                 665                 670         


Lys Arg Arg Val Ala Phe Phe Ala His Gly Arg Ile Arg Arg Lys Val 
        675                 680                 685             


Thr Glu Lys Ala Val Glu Arg Gly Met Arg Val Val Thr Val Pro Tyr 
    690                 695                 700                 


Leu Ala Ser Ser Lys Val Cys Ala Glu Cys Arg Lys Lys Gln Lys Asp 
705                 710                 715                 720 


Asn Lys Gln Trp Glu Lys Asn Lys Lys Arg Gly Leu Phe Lys Cys Glu 
                725                 730                 735     


Gly Cys Gly Ser Gln Ala Gln Val Asp Glu Asn Ala Ala Arg Val Leu 
            740                 745                 750         


Gly Arg Val Phe Trp Gly Glu Ile Glu Leu Pro Thr Ala Ile Pro 
        755                 760                 765         


<210>  258
<211>  870
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12h1 polypeptide sequence

<400>  258

Met Lys Val His Glu Ile Pro Arg Ser Gln Leu Leu Lys Ile Lys Gln 
1               5                   10                  15      


Tyr Glu Gly Ser Phe Val Glu Trp Tyr Arg Asp Leu Gln Glu Asp Arg 
            20                  25                  30          


Lys Lys Phe Ala Ser Leu Leu Phe Arg Trp Ala Ala Phe Gly Tyr Ala 
        35                  40                  45              


Ala Arg Glu Asp Asp Gly Ala Thr Tyr Ile Ser Pro Ser Gln Ala Leu 
    50                  55                  60                  


Leu Glu Arg Arg Leu Leu Leu Gly Asp Ala Glu Asp Val Ala Ile Lys 
65                  70                  75                  80  


Phe Leu Asp Val Leu Phe Lys Gly Gly Ala Pro Ser Ser Ser Cys Tyr 
                85                  90                  95      


Ser Leu Phe Tyr Glu Asp Phe Ala Leu Arg Asp Lys Ala Lys Tyr Ser 
            100                 105                 110         


Gly Ala Lys Arg Glu Phe Ile Glu Gly Leu Ala Thr Met Pro Leu Asp 
        115                 120                 125             


Lys Ile Ile Glu Arg Ile Arg Gln Asp Glu Gln Leu Ser Lys Ile Pro 
    130                 135                 140                 


Ala Glu Glu Trp Leu Ile Leu Gly Ala Glu Tyr Ser Pro Glu Glu Ile 
145                 150                 155                 160 


Trp Glu Gln Val Ala Pro Arg Ile Val Asn Val Asp Arg Ser Leu Gly 
                165                 170                 175     


Lys Gln Leu Arg Glu Arg Leu Gly Ile Lys Cys Arg Arg Pro His Asp 
            180                 185                 190         


Ala Gly Tyr Cys Lys Ile Leu Met Glu Val Val Ala Arg Gln Leu Arg 
        195                 200                 205             


Ser His Asn Glu Thr Tyr His Glu Tyr Leu Asn Gln Thr His Glu Met 
    210                 215                 220                 


Lys Thr Lys Val Ala Asn Asn Leu Thr Asn Glu Phe Asp Leu Val Cys 
225                 230                 235                 240 


Glu Phe Ala Glu Val Leu Glu Glu Lys Asn Tyr Gly Leu Gly Trp Tyr 
                245                 250                 255     


Val Leu Trp Gln Gly Val Lys Gln Ala Leu Lys Glu Gln Lys Lys Pro 
            260                 265                 270         


Thr Lys Ile Gln Ile Ala Val Asp Gln Leu Arg Gln Pro Lys Phe Ala 
        275                 280                 285             


Gly Leu Leu Thr Ala Lys Trp Arg Ala Leu Lys Gly Ala Tyr Asp Thr 
    290                 295                 300                 


Trp Lys Leu Lys Lys Arg Leu Glu Lys Arg Lys Ala Phe Pro Tyr Met 
305                 310                 315                 320 


Pro Asn Trp Asp Asn Asp Tyr Gln Ile Pro Val Gly Leu Thr Gly Leu 
                325                 330                 335     


Gly Val Phe Thr Leu Glu Val Lys Arg Thr Glu Val Val Val Asp Leu 
            340                 345                 350         


Lys Glu His Gly Lys Leu Phe Cys Ser His Ser His Tyr Phe Gly Asp 
        355                 360                 365             


Leu Thr Ala Glu Lys His Pro Ser Arg Tyr His Leu Lys Phe Arg His 
    370                 375                 380                 


Lys Leu Lys Leu Arg Lys Arg Asp Ser Arg Val Glu Pro Thr Ile Gly 
385                 390                 395                 400 


Pro Trp Ile Glu Ala Ala Leu Arg Glu Ile Thr Ile Gln Lys Lys Pro 
                405                 410                 415     


Asn Gly Val Phe Tyr Leu Gly Leu Pro Tyr Ala Leu Ser His Gly Ile 
            420                 425                 430         


Asp Asn Phe Gln Ile Ala Lys Arg Phe Phe Ser Ala Ala Lys Pro Asp 
        435                 440                 445             


Lys Glu Val Ile Asn Gly Leu Pro Ser Glu Met Val Val Gly Ala Ala 
    450                 455                 460                 


Asp Leu Asn Leu Ser Asn Ile Val Ala Pro Val Lys Ala Arg Ile Gly 
465                 470                 475                 480 


Lys Gly Leu Glu Gly Pro Leu His Ala Leu Asp Tyr Gly Tyr Gly Glu 
                485                 490                 495     


Leu Ile Asp Gly Pro Lys Ile Leu Thr Pro Asp Gly Pro Arg Cys Gly 
            500                 505                 510         


Glu Leu Ile Ser Leu Lys Arg Asp Ile Val Glu Ile Lys Ser Ala Ile 
        515                 520                 525             


Lys Glu Phe Lys Ala Cys Gln Arg Glu Gly Leu Thr Met Ser Glu Glu 
    530                 535                 540                 


Thr Thr Thr Trp Leu Ser Glu Val Glu Ser Pro Ser Asp Ser Pro Arg 
545                 550                 555                 560 


Cys Met Ile Gln Ser Arg Ile Ala Asp Thr Ser Arg Arg Leu Asn Ser 
                565                 570                 575     


Phe Lys Tyr Gln Met Asn Lys Glu Gly Tyr Gln Asp Leu Ala Glu Ala 
            580                 585                 590         


Leu Arg Leu Leu Asp Ala Met Asp Ser Tyr Asn Ser Leu Leu Glu Ser 
        595                 600                 605             


Tyr Gln Arg Met His Leu Ser Pro Gly Glu Gln Ser Pro Lys Glu Ala 
    610                 615                 620                 


Lys Phe Asp Thr Lys Arg Ala Ser Phe Arg Asp Leu Leu Arg Arg Arg 
625                 630                 635                 640 


Val Ala His Thr Ile Val Glu Tyr Phe Asp Asp Cys Asp Ile Val Phe 
                645                 650                 655     


Phe Glu Asp Leu Asp Gly Pro Ser Asp Ser Asp Ser Arg Asn Asn Ala 
            660                 665                 670         


Leu Val Lys Leu Leu Ser Pro Arg Thr Leu Leu Leu Tyr Ile Arg Gln 
        675                 680                 685             


Ala Leu Glu Lys Arg Gly Ile Gly Met Val Glu Val Ala Lys Asp Gly 
    690                 695                 700                 


Thr Ser Gln Asn Asn Pro Ile Ser Gly His Val Gly Trp Arg Asn Lys 
705                 710                 715                 720 


Gln Asn Lys Ser Glu Ile Tyr Phe Tyr Glu Asp Lys Glu Leu Leu Val 
                725                 730                 735     


Met Asp Ala Asp Glu Val Gly Ala Met Asn Ile Leu Cys Arg Gly Leu 
            740                 745                 750         


Asn His Ser Val Cys Pro Tyr Ser Phe Val Thr Lys Ala Pro Glu Lys 
        755                 760                 765             


Lys Asn Asp Glu Lys Lys Glu Gly Asp Tyr Gly Lys Arg Val Lys Arg 
    770                 775                 780                 


Phe Leu Lys Asp Arg Tyr Gly Ser Ser Asn Val Arg Phe Leu Val Ala 
785                 790                 795                 800 


Ser Met Gly Phe Val Thr Val Thr Thr Lys Arg Pro Lys Asp Ala Leu 
                805                 810                 815     


Val Gly Lys Arg Leu Tyr Tyr His Gly Gly Glu Leu Val Thr His Asp 
            820                 825                 830         


Leu His Asn Arg Met Lys Asp Glu Ile Lys Tyr Leu Val Glu Lys Glu 
        835                 840                 845             


Val Leu Ala Arg Arg Val Ser Leu Ser Asp Ser Thr Ile Lys Ser Tyr 
    850                 855                 860                 


Lys Ser Phe Ala His Val 
865                 870 


<210>  259
<211>  1093
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12i1 polypeptide sequence

<400>  259

Met Ser Asn Lys Glu Lys Asn Ala Ser Glu Thr Arg Lys Ala Tyr Thr 
1               5                   10                  15      


Thr Lys Met Ile Pro Arg Ser His Asp Arg Met Lys Leu Leu Gly Asn 
            20                  25                  30          


Phe Met Asp Tyr Leu Met Asp Gly Thr Pro Ile Phe Phe Glu Leu Trp 
        35                  40                  45              


Asn Gln Phe Gly Gly Gly Ile Asp Arg Asp Ile Ile Ser Gly Thr Ala 
    50                  55                  60                  


Asn Lys Asp Lys Ile Ser Asp Asp Leu Leu Leu Ala Val Asn Trp Phe 
65                  70                  75                  80  


Lys Val Met Pro Ile Asn Ser Lys Pro Gln Gly Val Ser Pro Ser Asn 
                85                  90                  95      


Leu Ala Asn Leu Phe Gln Gln Tyr Ser Gly Ser Glu Pro Asp Ile Gln 
            100                 105                 110         


Ala Gln Glu Tyr Phe Ala Ser Asn Phe Asp Thr Glu Lys His Gln Trp 
        115                 120                 125             


Lys Asp Met Arg Val Glu Tyr Glu Arg Leu Leu Ala Glu Leu Gln Leu 
    130                 135                 140                 


Ser Arg Ser Asp Met His His Asp Leu Lys Leu Met Tyr Lys Glu Lys 
145                 150                 155                 160 


Cys Ile Gly Leu Ser Leu Ser Thr Ala His Tyr Ile Thr Ser Val Met 
                165                 170                 175     


Phe Gly Thr Gly Ala Lys Asn Asn Arg Gln Thr Lys His Gln Phe Tyr 
            180                 185                 190         


Ser Lys Val Ile Gln Leu Leu Glu Glu Ser Thr Gln Ile Asn Ser Val 
        195                 200                 205             


Glu Gln Leu Ala Ser Ile Ile Leu Lys Ala Gly Asp Cys Asp Ser Tyr 
    210                 215                 220                 


Arg Lys Leu Arg Ile Arg Cys Ser Arg Lys Gly Ala Thr Pro Ser Ile 
225                 230                 235                 240 


Leu Lys Ile Val Gln Asp Tyr Glu Leu Gly Thr Asn His Asp Asp Glu 
                245                 250                 255     


Val Asn Val Pro Ser Leu Ile Ala Asn Leu Lys Glu Lys Leu Gly Arg 
            260                 265                 270         


Phe Glu Tyr Glu Cys Glu Trp Lys Cys Met Glu Lys Ile Lys Ala Phe 
        275                 280                 285             


Leu Ala Ser Lys Val Gly Pro Tyr Tyr Leu Gly Ser Tyr Ser Ala Met 
    290                 295                 300                 


Leu Glu Asn Ala Leu Ser Pro Ile Lys Gly Met Thr Thr Lys Asn Cys 
305                 310                 315                 320 


Lys Phe Val Leu Lys Gln Ile Asp Ala Lys Asn Asp Ile Lys Tyr Glu 
                325                 330                 335     


Asn Glu Pro Phe Gly Lys Ile Val Glu Gly Phe Phe Asp Ser Pro Tyr 
            340                 345                 350         


Phe Glu Ser Asp Thr Asn Val Lys Trp Val Leu His Pro His His Ile 
        355                 360                 365             


Gly Glu Ser Asn Ile Lys Thr Leu Trp Glu Asp Leu Asn Ala Ile His 
    370                 375                 380                 


Ser Lys Tyr Glu Glu Asp Ile Ala Ser Leu Ser Glu Asp Lys Lys Glu 
385                 390                 395                 400 


Lys Arg Ile Lys Val Tyr Gln Gly Asp Val Cys Gln Thr Ile Asn Thr 
                405                 410                 415     


Tyr Cys Glu Glu Val Gly Lys Glu Ala Lys Thr Pro Leu Val Gln Leu 
            420                 425                 430         


Leu Arg Tyr Leu Tyr Ser Arg Lys Asp Asp Ile Ala Val Asp Lys Ile 
        435                 440                 445             


Ile Asp Gly Ile Thr Phe Leu Ser Lys Lys His Lys Val Glu Lys Gln 
    450                 455                 460                 


Lys Ile Asn Pro Val Ile Gln Lys Tyr Pro Ser Phe Asn Phe Gly Asn 
465                 470                 475                 480 


Asn Ser Lys Leu Leu Gly Lys Ile Ile Ser Pro Lys Asp Lys Leu Lys 
                485                 490                 495     


His Asn Leu Lys Cys Asn Arg Asn Gln Val Asp Asn Tyr Ile Trp Ile 
            500                 505                 510         


Glu Ile Lys Val Leu Asn Thr Lys Thr Met Arg Trp Glu Lys His His 
        515                 520                 525             


Tyr Ala Leu Ser Ser Thr Arg Phe Leu Glu Glu Val Tyr Tyr Pro Ala 
    530                 535                 540                 


Thr Ser Glu Asn Pro Pro Asp Ala Leu Ala Ala Arg Phe Arg Thr Lys 
545                 550                 555                 560 


Thr Asn Gly Tyr Glu Gly Lys Pro Ala Leu Ser Ala Glu Gln Ile Glu 
                565                 570                 575     


Gln Ile Arg Ser Ala Pro Val Gly Leu Arg Lys Val Lys Lys Arg Gln 
            580                 585                 590         


Met Arg Leu Glu Ala Ala Arg Gln Gln Asn Leu Leu Pro Arg Tyr Thr 
        595                 600                 605             


Trp Gly Lys Asp Phe Asn Ile Asn Ile Cys Lys Arg Gly Asn Asn Phe 
    610                 615                 620                 


Glu Val Thr Leu Ala Thr Lys Val Lys Lys Lys Lys Glu Lys Asn Tyr 
625                 630                 635                 640 


Lys Val Val Leu Gly Tyr Asp Ala Asn Ile Val Arg Lys Asn Thr Tyr 
                645                 650                 655     


Ala Ala Ile Glu Ala His Ala Asn Gly Asp Gly Val Ile Asp Tyr Asn 
            660                 665                 670         


Asp Leu Pro Val Lys Pro Ile Glu Ser Gly Phe Val Thr Val Glu Ser 
        675                 680                 685             


Gln Val Arg Asp Lys Ser Tyr Asp Gln Leu Ser Tyr Asn Gly Val Lys 
    690                 695                 700                 


Leu Leu Tyr Cys Lys Pro His Val Glu Ser Arg Arg Ser Phe Leu Glu 
705                 710                 715                 720 


Lys Tyr Arg Asn Gly Thr Met Lys Asp Asn Arg Gly Asn Asn Ile Gln 
                725                 730                 735     


Ile Asp Phe Met Lys Asp Phe Glu Ala Ile Ala Asp Asp Glu Thr Ser 
            740                 745                 750         


Leu Tyr Tyr Phe Asn Met Lys Tyr Cys Lys Leu Leu Gln Ser Ser Ile 
        755                 760                 765             


Arg Asn His Ser Ser Gln Ala Lys Glu Tyr Arg Glu Glu Ile Phe Glu 
    770                 775                 780                 


Leu Leu Arg Asp Gly Lys Leu Ser Val Leu Lys Leu Ser Ser Leu Ser 
785                 790                 795                 800 


Asn Leu Ser Phe Val Met Phe Lys Val Ala Lys Ser Leu Ile Gly Thr 
                805                 810                 815     


Tyr Phe Gly His Leu Leu Lys Lys Pro Lys Asn Ser Lys Ser Asp Val 
            820                 825                 830         


Lys Ala Pro Pro Ile Thr Asp Glu Asp Lys Gln Lys Ala Asp Pro Glu 
        835                 840                 845             


Met Phe Ala Leu Arg Leu Ala Leu Glu Glu Lys Arg Leu Asn Lys Val 
    850                 855                 860                 


Lys Ser Lys Lys Glu Val Ile Ala Asn Lys Ile Val Ala Lys Ala Leu 
865                 870                 875                 880 


Glu Leu Arg Asp Lys Tyr Gly Pro Val Leu Ile Lys Gly Glu Asn Ile 
                885                 890                 895     


Ser Asp Thr Thr Lys Lys Gly Lys Lys Ser Ser Thr Asn Ser Phe Leu 
            900                 905                 910         


Met Asp Trp Leu Ala Arg Gly Val Ala Asn Lys Val Lys Glu Met Val 
        915                 920                 925             


Met Met His Gln Gly Leu Glu Phe Val Glu Val Asn Pro Asn Phe Thr 
    930                 935                 940                 


Ser His Gln Asp Pro Phe Val His Lys Asn Pro Glu Asn Thr Phe Arg 
945                 950                 955                 960 


Ala Arg Tyr Ser Arg Cys Thr Pro Ser Glu Leu Thr Glu Lys Asn Arg 
                965                 970                 975     


Lys Glu Ile Leu Ser Phe Leu Ser Asp Lys Pro Ser Lys Arg Pro Thr 
            980                 985                 990         


Asn Ala Tyr Tyr Asn Glu Gly Ala  Met Ala Phe Leu Ala  Thr Tyr Gly 
        995                 1000                 1005             


Leu Lys  Lys Asn Asp Val Leu  Gly Val Ser Leu Glu  Lys Phe Lys 
    1010                 1015                 1020             


Gln Ile  Met Ala Asn Ile Leu  His Gln Arg Ser Glu  Asp Gln Leu 
    1025                 1030                 1035             


Leu Phe  Pro Ser Arg Gly Gly  Met Phe Tyr Leu Ala  Thr Tyr Lys 
    1040                 1045                 1050             


Leu Asp  Ala Asp Ala Thr Ser  Val Asn Trp Asn Gly  Lys Gln Phe 
    1055                 1060                 1065             


Trp Val  Cys Asn Ala Asp Leu  Val Ala Ala Tyr Asn  Val Gly Leu 
    1070                 1075                 1080             


Val Asp  Ile Gln Lys Asp Phe  Lys Lys Lys 
    1085                 1090             


<210>  260
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cas12i2 polypeptide sequence

<400>  260

Met Ser Ser Ala Ile Lys Ser Tyr Lys Ser Val Leu Arg Pro Asn Glu 
1               5                   10                  15      


Arg Lys Asn Gln Leu Leu Lys Ser Thr Ile Gln Cys Leu Glu Asp Gly 
            20                  25                  30          


Ser Ala Phe Phe Phe Lys Met Leu Gln Gly Leu Phe Gly Gly Ile Thr 
        35                  40                  45              


Pro Glu Ile Val Arg Phe Ser Thr Glu Gln Glu Lys Gln Gln Gln Asp 
    50                  55                  60                  


Ile Ala Leu Trp Cys Ala Val Asn Trp Phe Arg Pro Val Ser Gln Asp 
65                  70                  75                  80  


Ser Leu Thr His Thr Ile Ala Ser Asp Asn Leu Val Glu Lys Phe Glu 
                85                  90                  95      


Glu Tyr Tyr Gly Gly Thr Ala Ser Asp Ala Ile Lys Gln Tyr Phe Ser 
            100                 105                 110         


Ala Ser Ile Gly Glu Ser Tyr Tyr Trp Asn Asp Cys Arg Gln Gln Tyr 
        115                 120                 125             


Tyr Asp Leu Cys Arg Glu Leu Gly Val Glu Val Ser Asp Leu Thr His 
    130                 135                 140                 


Asp Leu Glu Ile Leu Cys Arg Glu Lys Cys Leu Ala Val Ala Thr Glu 
145                 150                 155                 160 


Ser Asn Gln Asn Asn Ser Ile Ile Ser Val Leu Phe Gly Thr Gly Glu 
                165                 170                 175     


Lys Glu Asp Arg Ser Val Lys Leu Arg Ile Thr Lys Lys Ile Leu Glu 
            180                 185                 190         


Ala Ile Ser Asn Leu Lys Glu Ile Pro Lys Asn Val Ala Pro Ile Gln 
        195                 200                 205             


Glu Ile Ile Leu Asn Val Ala Lys Ala Thr Lys Glu Thr Phe Arg Gln 
    210                 215                 220                 


Val Tyr Ala Gly Asn Leu Gly Ala Pro Ser Thr Leu Glu Lys Phe Ile 
225                 230                 235                 240 


Ala Lys Asp Gly Gln Lys Glu Phe Asp Leu Lys Lys Leu Gln Thr Asp 
                245                 250                 255     


Leu Lys Lys Val Ile Arg Gly Lys Ser Lys Glu Arg Asp Trp Cys Cys 
            260                 265                 270         


Gln Glu Glu Leu Arg Ser Tyr Val Glu Gln Asn Thr Ile Gln Tyr Asp 
        275                 280                 285             


Leu Trp Ala Trp Gly Glu Met Phe Asn Lys Ala His Thr Ala Leu Lys 
    290                 295                 300                 


Ile Lys Ser Thr Arg Asn Tyr Asn Phe Ala Lys Gln Arg Leu Glu Gln 
305                 310                 315                 320 


Phe Lys Glu Ile Gln Ser Leu Asn Asn Leu Leu Val Val Lys Lys Leu 
                325                 330                 335     


Asn Asp Phe Phe Asp Ser Glu Phe Phe Ser Gly Glu Glu Thr Tyr Thr 
            340                 345                 350         


Ile Cys Val His His Leu Gly Gly Lys Asp Leu Ser Lys Leu Tyr Lys 
        355                 360                 365             


Ala Trp Glu Asp Asp Pro Ala Asp Pro Glu Asn Ala Ile Val Val Leu 
    370                 375                 380                 


Cys Asp Asp Leu Lys Asn Asn Phe Lys Lys Glu Pro Ile Arg Asn Ile 
385                 390                 395                 400 


Leu Arg Tyr Ile Phe Thr Ile Arg Gln Glu Cys Ser Ala Gln Asp Ile 
                405                 410                 415     


Leu Ala Ala Ala Lys Tyr Asn Gln Gln Leu Asp Arg Tyr Lys Ser Gln 
            420                 425                 430         


Lys Ala Asn Pro Ser Val Leu Gly Asn Gln Gly Phe Thr Trp Thr Asn 
        435                 440                 445             


Ala Val Ile Leu Pro Glu Lys Ala Gln Arg Asn Asp Arg Pro Asn Ser 
    450                 455                 460                 


Leu Asp Leu Arg Ile Trp Leu Tyr Leu Lys Leu Arg His Pro Asp Gly 
465                 470                 475                 480 


Arg Trp Lys Lys His His Ile Pro Phe Tyr Asp Thr Arg Phe Phe Gln 
                485                 490                 495     


Glu Ile Tyr Ala Ala Gly Asn Ser Pro Val Asp Thr Cys Gln Phe Arg 
            500                 505                 510         


Thr Pro Arg Phe Gly Tyr His Leu Pro Lys Leu Thr Asp Gln Thr Ala 
        515                 520                 525             


Ile Arg Val Asn Lys Lys His Val Lys Ala Ala Lys Thr Glu Ala Arg 
    530                 535                 540                 


Ile Arg Leu Ala Ile Gln Gln Gly Thr Leu Pro Val Ser Asn Leu Lys 
545                 550                 555                 560 


Ile Thr Glu Ile Ser Ala Thr Ile Asn Ser Lys Gly Gln Val Arg Ile 
                565                 570                 575     


Pro Val Lys Phe Asp Val Gly Arg Gln Lys Gly Thr Leu Gln Ile Gly 
            580                 585                 590         


Asp Arg Phe Cys Gly Tyr Asp Gln Asn Gln Thr Ala Ser His Ala Tyr 
        595                 600                 605             


Ser Leu Trp Glu Val Val Lys Glu Gly Gln Tyr His Lys Glu Leu Gly 
    610                 615                 620                 


Cys Phe Val Arg Phe Ile Ser Ser Gly Asp Ile Val Ser Ile Thr Glu 
625                 630                 635                 640 


Asn Arg Gly Asn Gln Phe Asp Gln Leu Ser Tyr Glu Gly Leu Ala Tyr 
                645                 650                 655     


Pro Gln Tyr Ala Asp Trp Arg Lys Lys Ala Ser Lys Phe Val Ser Leu 
            660                 665                 670         


Trp Gln Ile Thr Lys Lys Asn Lys Lys Lys Glu Ile Val Thr Val Glu 
        675                 680                 685             


Ala Lys Glu Lys Phe Asp Ala Ile Cys Lys Tyr Gln Pro Arg Leu Tyr 
    690                 695                 700                 


Lys Phe Asn Lys Glu Tyr Ala Tyr Leu Leu Arg Asp Ile Val Arg Gly 
705                 710                 715                 720 


Lys Ser Leu Val Glu Leu Gln Gln Ile Arg Gln Glu Ile Phe Arg Phe 
                725                 730                 735     


Ile Glu Gln Asp Cys Gly Val Thr Arg Leu Gly Ser Leu Ser Leu Ser 
            740                 745                 750         


Thr Leu Glu Thr Val Lys Ala Val Lys Gly Ile Ile Tyr Ser Tyr Phe 
        755                 760                 765             


Ser Thr Ala Leu Asn Ala Ser Lys Asn Asn Pro Ile Ser Asp Glu Gln 
    770                 775                 780                 


Arg Lys Glu Phe Asp Pro Glu Leu Phe Ala Leu Leu Glu Lys Leu Glu 
785                 790                 795                 800 


Leu Ile Arg Thr Arg Lys Lys Lys Gln Lys Val Glu Arg Ile Ala Asn 
                805                 810                 815     


Ser Leu Ile Gln Thr Cys Leu Glu Asn Asn Ile Lys Phe Ile Arg Gly 
            820                 825                 830         


Glu Gly Asp Leu Ser Thr Thr Asn Asn Ala Thr Lys Lys Lys Ala Asn 
        835                 840                 845             


Ser Arg Ser Met Asp Trp Leu Ala Arg Gly Val Phe Asn Lys Ile Arg 
    850                 855                 860                 


Gln Leu Ala Pro Met His Asn Ile Thr Leu Phe Gly Cys Gly Ser Leu 
865                 870                 875                 880 


Tyr Thr Ser His Gln Asp Pro Leu Val His Arg Asn Pro Asp Lys Ala 
                885                 890                 895     


Met Lys Cys Arg Trp Ala Ala Ile Pro Val Lys Asp Ile Gly Asp Trp 
            900                 905                 910         


Val Leu Arg Lys Leu Ser Gln Asn Leu Arg Ala Lys Asn Ile Gly Thr 
        915                 920                 925             


Gly Glu Tyr Tyr His Gln Gly Val Lys Glu Phe Leu Ser His Tyr Glu 
    930                 935                 940                 


Leu Gln Asp Leu Glu Glu Glu Leu Leu Lys Trp Arg Ser Asp Arg Lys 
945                 950                 955                 960 


Ser Asn Ile Pro Cys Trp Val Leu Gln Asn Arg Leu Ala Glu Lys Leu 
                965                 970                 975     


Gly Asn Lys Glu Ala Val Val Tyr Ile Pro Val Arg Gly Gly Arg Ile 
            980                 985                 990         


Tyr Phe Ala Thr His Lys Val Ala  Thr Gly Ala Val Ser  Ile Val Phe 
        995                 1000                 1005             


Asp Gln  Lys Gln Val Trp Val  Cys Asn Ala Asp His  Val Ala Ala 
    1010                 1015                 1020             


Ala Asn  Ile Ala Leu Thr Val  Lys Gly Ile Gly Glu  Gln Ser Ser 
    1025                 1030                 1035             


Asp Glu  Glu Asn Pro Asp Gly  Ser Arg Ile Lys Leu  Gln Leu Thr 
    1040                 1045                 1050             


Ser 
    


<210>  261
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  261

Gly Gly Gly Ser 
1               


<210>  262
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  262

Gly Gly Gly Gly Ser 
1               5   


<210>  263
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  263

Glu Ala Ala Ala Lys 
1               5   


<210>  264
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  264

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


<210>  265
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: linker peptide sequence

<400>  265

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  266
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  266

Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
1               5                   10                  15      


Glu Ser Ser Gly 
            20  


<210>  267
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  267
ggaggctctg gaggaagc                                                     18


<210>  268
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  268
ggctcttctg gatctgaaac acctggcaca agcgagagcg ccacccctga gagctctggc       60


<210>  269
<211>  1129
<212>  PRT
<213>  Alicyclobacillus acidiphilus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1129)
<223>  AacCas12b polypeptide sequence

<400>  269

Met Ala Val Lys Ser Met Lys Val Lys Leu Arg Leu Asp Asn Met Pro 
1               5                   10                  15      


Glu Ile Arg Ala Gly Leu Trp Lys Leu His Thr Glu Val Asn Ala Gly 
            20                  25                  30          


Val Arg Tyr Tyr Thr Glu Trp Leu Ser Leu Leu Arg Gln Glu Asn Leu 
        35                  40                  45              


Tyr Arg Arg Ser Pro Asn Gly Asp Gly Glu Gln Glu Cys Tyr Lys Thr 
    50                  55                  60                  


Ala Glu Glu Cys Lys Ala Glu Leu Leu Glu Arg Leu Arg Ala Arg Gln 
65                  70                  75                  80  


Val Glu Asn Gly His Cys Gly Pro Ala Gly Ser Asp Asp Glu Leu Leu 
                85                  90                  95      


Gln Leu Ala Arg Gln Leu Tyr Glu Leu Leu Val Pro Gln Ala Ile Gly 
            100                 105                 110         


Ala Lys Gly Asp Ala Gln Gln Ile Ala Arg Lys Phe Leu Ser Pro Leu 
        115                 120                 125             


Ala Asp Lys Asp Ala Val Gly Gly Leu Gly Ile Ala Lys Ala Gly Asn 
    130                 135                 140                 


Lys Pro Arg Trp Val Arg Met Arg Glu Ala Gly Glu Pro Gly Trp Glu 
145                 150                 155                 160 


Glu Glu Lys Ala Lys Ala Glu Ala Arg Lys Ser Thr Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Leu Arg Ala Leu Ala Asp Phe Gly Leu Lys Pro Leu Met Arg 
            180                 185                 190         


Val Tyr Thr Asp Ser Asp Met Ser Ser Val Gln Trp Lys Pro Leu Arg 
        195                 200                 205             


Lys Gly Gln Ala Val Arg Thr Trp Asp Arg Asp Met Phe Gln Gln Ala 
    210                 215                 220                 


Ile Glu Arg Met Met Ser Trp Glu Ser Trp Asn Gln Arg Val Gly Glu 
225                 230                 235                 240 


Ala Tyr Ala Lys Leu Val Glu Gln Lys Ser Arg Phe Glu Gln Lys Asn 
                245                 250                 255     


Phe Val Gly Gln Glu His Leu Val Gln Leu Val Asn Gln Leu Gln Gln 
            260                 265                 270         


Asp Met Lys Glu Ala Ser His Gly Leu Glu Ser Lys Glu Gln Thr Ala 
        275                 280                 285             


His Tyr Leu Thr Gly Arg Ala Leu Arg Gly Ser Asp Lys Val Phe Glu 
    290                 295                 300                 


Lys Trp Glu Lys Leu Asp Pro Asp Ala Pro Phe Asp Leu Tyr Asp Thr 
305                 310                 315                 320 


Glu Ile Lys Asn Val Gln Arg Arg Asn Thr Arg Arg Phe Gly Ser His 
                325                 330                 335     


Asp Leu Phe Ala Lys Leu Ala Glu Pro Lys Tyr Gln Ala Leu Trp Arg 
            340                 345                 350         


Glu Asp Ala Ser Phe Leu Thr Arg Tyr Ala Val Tyr Asn Ser Ile Val 
        355                 360                 365             


Arg Lys Leu Asn His Ala Lys Met Phe Ala Thr Phe Thr Leu Pro Asp 
    370                 375                 380                 


Ala Thr Ala His Pro Ile Trp Thr Arg Phe Asp Lys Leu Gly Gly Asn 
385                 390                 395                 400 


Leu His Gln Tyr Thr Phe Leu Phe Asn Glu Phe Gly Glu Gly Arg His 
                405                 410                 415     


Ala Ile Arg Phe Gln Lys Leu Leu Thr Val Glu Asp Gly Val Ala Lys 
            420                 425                 430         


Glu Val Asp Asp Val Thr Val Pro Ile Ser Met Ser Ala Gln Leu Asp 
        435                 440                 445             


Asp Leu Leu Pro Arg Asp Pro His Glu Leu Val Ala Leu Tyr Phe Gln 
    450                 455                 460                 


Asp Tyr Gly Ala Glu Gln His Leu Ala Gly Glu Phe Gly Gly Ala Lys 
465                 470                 475                 480 


Ile Gln Tyr Arg Arg Asp Gln Leu Asn His Leu His Ala Arg Arg Gly 
                485                 490                 495     


Ala Arg Asp Val Tyr Leu Asn Leu Ser Val Arg Val Gln Ser Gln Ser 
            500                 505                 510         


Glu Ala Arg Gly Glu Arg Arg Pro Pro Tyr Ala Ala Val Phe Arg Leu 
        515                 520                 525             


Val Gly Asp Asn His Arg Ala Phe Val His Phe Asp Lys Leu Ser Asp 
    530                 535                 540                 


Tyr Leu Ala Glu His Pro Asp Asp Gly Lys Leu Gly Ser Glu Gly Leu 
545                 550                 555                 560 


Leu Ser Gly Leu Arg Val Met Ser Val Asp Leu Gly Leu Arg Thr Ser 
                565                 570                 575     


Ala Ser Ile Ser Val Phe Arg Val Ala Arg Lys Asp Glu Leu Lys Pro 
            580                 585                 590         


Asn Ser Glu Gly Arg Val Pro Phe Cys Phe Pro Ile Glu Gly Asn Glu 
        595                 600                 605             


Asn Leu Val Ala Val His Glu Arg Ser Gln Leu Leu Lys Leu Pro Gly 
    610                 615                 620                 


Glu Thr Glu Ser Lys Asp Leu Arg Ala Ile Arg Glu Glu Arg Gln Arg 
625                 630                 635                 640 


Thr Leu Arg Gln Leu Arg Thr Gln Leu Ala Tyr Leu Arg Leu Leu Val 
                645                 650                 655     


Arg Cys Gly Ser Glu Asp Val Gly Arg Arg Glu Arg Ser Trp Ala Lys 
            660                 665                 670         


Leu Ile Glu Gln Pro Met Asp Ala Asn Gln Met Thr Pro Asp Trp Arg 
        675                 680                 685             


Glu Ala Phe Glu Asp Glu Leu Gln Lys Leu Lys Ser Leu Tyr Gly Ile 
    690                 695                 700                 


Cys Gly Asp Arg Glu Trp Thr Glu Ala Val Tyr Glu Ser Val Arg Arg 
705                 710                 715                 720 


Val Trp Arg His Met Gly Lys Gln Val Arg Asp Trp Arg Lys Asp Val 
                725                 730                 735     


Arg Ser Gly Glu Arg Pro Lys Ile Arg Gly Tyr Gln Lys Asp Val Val 
            740                 745                 750         


Gly Gly Asn Ser Ile Glu Gln Ile Glu Tyr Leu Glu Arg Gln Tyr Lys 
        755                 760                 765             


Phe Leu Lys Ser Trp Ser Phe Phe Gly Lys Val Ser Gly Gln Val Ile 
    770                 775                 780                 


Arg Ala Glu Lys Gly Ser Arg Phe Ala Ile Thr Leu Arg Glu His Ile 
785                 790                 795                 800 


Asp His Ala Lys Glu Asp Arg Leu Lys Lys Leu Ala Asp Arg Ile Ile 
                805                 810                 815     


Met Glu Ala Leu Gly Tyr Val Tyr Ala Leu Asp Asp Glu Arg Gly Lys 
            820                 825                 830         


Gly Lys Trp Val Ala Lys Tyr Pro Pro Cys Gln Leu Ile Leu Leu Glu 
        835                 840                 845             


Glu Leu Ser Glu Tyr Gln Phe Asn Asn Asp Arg Pro Pro Ser Glu Asn 
    850                 855                 860                 


Asn Gln Leu Met Gln Trp Ser His Arg Gly Val Phe Gln Glu Leu Leu 
865                 870                 875                 880 


Asn Gln Ala Gln Val His Asp Leu Leu Val Gly Thr Met Tyr Ala Ala 
                885                 890                 895     


Phe Ser Ser Arg Phe Asp Ala Arg Thr Gly Ala Pro Gly Ile Arg Cys 
            900                 905                 910         


Arg Arg Val Pro Ala Arg Cys Ala Arg Glu Gln Asn Pro Glu Pro Phe 
        915                 920                 925             


Pro Trp Trp Leu Asn Lys Phe Val Ala Glu His Lys Leu Asp Gly Cys 
    930                 935                 940                 


Pro Leu Arg Ala Asp Asp Leu Ile Pro Thr Gly Glu Gly Glu Phe Phe 
945                 950                 955                 960 


Val Ser Pro Phe Ser Ala Glu Glu Gly Asp Phe His Gln Ile His Ala 
                965                 970                 975     


Asp Leu Asn Ala Ala Gln Asn Leu Gln Arg Arg Leu Trp Ser Asp Phe 
            980                 985                 990         


Asp Ile Ser Gln Ile Arg Leu Arg  Cys Asp Trp Gly Glu  Val Asp Gly 
        995                 1000                 1005             


Glu Pro  Val Leu Ile Pro Arg  Thr Thr Gly Lys Arg  Thr Ala Asp 
    1010                 1015                 1020             


Ser Tyr  Gly Asn Lys Val Phe  Tyr Thr Lys Thr Gly  Val Thr Tyr 
    1025                 1030                 1035             


Tyr Glu  Arg Glu Arg Gly Lys  Lys Arg Arg Lys Val  Phe Ala Gln 
    1040                 1045                 1050             


Glu Glu  Leu Ser Glu Glu Glu  Ala Glu Leu Leu Val  Glu Ala Asp 
    1055                 1060                 1065             


Glu Ala  Arg Glu Lys Ser Val  Val Leu Met Arg Asp  Pro Ser Gly 
    1070                 1075                 1080             


Ile Ile  Asn Arg Gly Asp Trp  Thr Arg Gln Lys Glu  Phe Trp Ser 
    1085                 1090                 1095             


Met Val  Asn Gln Arg Ile Glu  Gly Tyr Leu Val Lys  Gln Ile Arg 
    1100                 1105                 1110             


Ser Arg  Val Arg Leu Gln Glu  Ser Ala Cys Glu Asn  Thr Gly Asp 
    1115                 1120                 1125             


Ile 
    


<210>  270
<211>  1140
<212>  PRT
<213>  Bacillus hisashii


<220>
<221>  MISC_FEATURE
<222>  (1)..(1140)
<223>  BhCas12b polypeptide sequence

<400>  270

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
            20                  25                  30          


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
        35                  40                  45              


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
    50                  55                  60                  


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
65                  70                  75                  80  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
                85                  90                  95      


Ser Phe Thr His Glu Val Asp Lys Asp Glu Val Phe Asn Ile Leu Arg 
            100                 105                 110         


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
        115                 120                 125             


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
    130                 135                 140                 


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
145                 150                 155                 160 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
                165                 170                 175     


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
            180                 185                 190         


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Tyr Thr Asp 
        195                 200                 205             


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
    210                 215                 220                 


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
225                 230                 235                 240 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
                245                 250                 255     


Tyr Glu Lys Val Glu Lys Glu Tyr Lys Thr Leu Glu Glu Arg Ile Lys 
            260                 265                 270         


Glu Asp Ile Gln Ala Leu Lys Ala Leu Glu Gln Tyr Glu Lys Glu Arg 
        275                 280                 285             


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
    290                 295                 300                 


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
305                 310                 315                 320 


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
                325                 330                 335     


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
            340                 345                 350         


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
        355                 360                 365             


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
    370                 375                 380                 


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
385                 390                 395                 400 


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
                405                 410                 415     


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
            420                 425                 430         


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
        435                 440                 445             


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
    450                 455                 460                 


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
465                 470                 475                 480 


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
                485                 490                 495     


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
            500                 505                 510         


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
        515                 520                 525             


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
    530                 535                 540                 


Ile His Arg Asp Asp Phe Pro Lys Val Val Asn Phe Lys Pro Lys Glu 
545                 550                 555                 560 


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
                565                 570                 575     


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
            580                 585                 590         


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
        595                 600                 605             


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
    610                 615                 620                 


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
625                 630                 635                 640 


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
                645                 650                 655     


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
            660                 665                 670         


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
        675                 680                 685             


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
    690                 695                 700                 


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
705                 710                 715                 720 


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
                725                 730                 735     


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
            740                 745                 750         


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
        755                 760                 765             


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
    770                 775                 780                 


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
785                 790                 795                 800 


Asn Ala Leu Lys Glu Asp Arg Leu Lys Lys Met Ala Asn Thr Ile Ile 
                805                 810                 815     


Met His Ala Leu Gly Tyr Cys Tyr Asp Val Arg Lys Lys Lys Trp Gln 
            820                 825                 830         


Ala Lys Asn Pro Ala Cys Gln Ile Ile Leu Phe Glu Asp Leu Ser Asn 
        835                 840                 845             


Tyr Asn Pro Tyr Glu Glu Arg Ser Arg Phe Glu Asn Ser Lys Leu Met 
    850                 855                 860                 


Lys Trp Ser Arg Arg Glu Ile Pro Arg Gln Val Ala Leu Gln Gly Glu 
865                 870                 875                 880 


Ile Tyr Gly Leu Gln Val Gly Glu Val Gly Ala Gln Phe Ser Ser Arg 
                885                 890                 895     


Phe His Ala Lys Thr Gly Ser Pro Gly Ile Arg Cys Ser Val Val Thr 
            900                 905                 910         


Lys Glu Lys Leu Gln Asp Asn Arg Phe Phe Lys Asn Leu Gln Arg Glu 
        915                 920                 925             


Gly Arg Leu Thr Leu Asp Lys Ile Ala Val Leu Lys Glu Gly Asp Leu 
    930                 935                 940                 


Tyr Pro Asp Lys Gly Gly Glu Lys Phe Ile Ser Leu Ser Lys Asp Arg 
945                 950                 955                 960 


Lys Cys Val Thr Thr His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln 
                965                 970                 975     


Lys Arg Phe Trp Thr Arg Thr His Gly Phe Tyr Lys Val Tyr Cys Lys 
            980                 985                 990         


Ala Tyr Gln Val Asp Gly Gln Thr  Val Tyr Ile Pro Glu  Ser Lys Asp 
        995                 1000                 1005             


Gln Lys  Gln Lys Ile Ile Glu  Glu Phe Gly Glu Gly  Tyr Phe Ile 
    1010                 1015                 1020             


Leu Lys  Asp Gly Val Tyr Glu  Trp Val Asn Ala Gly  Lys Leu Lys 
    1025                 1030                 1035             


Ile Lys  Lys Gly Ser Ser Lys  Gln Ser Ser Ser Glu  Leu Val Asp 
    1040                 1045                 1050             


Ser Asp  Ile Leu Lys Asp Ser  Phe Asp Leu Ala Ser  Glu Leu Lys 
    1055                 1060                 1065             


Gly Glu  Lys Leu Met Leu Tyr  Arg Asp Pro Ser Gly  Asn Val Phe 
    1070                 1075                 1080             


Pro Ser  Asp Lys Trp Met Ala  Ala Gly Val Phe Phe  Gly Lys Leu 
    1085                 1090                 1095             


Glu Arg  Ile Leu Ile Ser Lys  Leu Thr Asn Gln Tyr  Ser Ile Ser 
    1100                 1105                 1110             


Thr Ile  Glu Asp Asp Ser Ser  Lys Gln Ser Met Lys  Arg Pro Ala 
    1115                 1120                 1125             


Ala Thr  Lys Lys Ala Gly Gln  Ala Lys Lys Lys Lys  
    1130                 1135                 1140 


<210>  271
<211>  1108
<212>  PRT
<213>  Bacillus thermoamylovorans


<220>
<221>  MISC_FEATURE
<222>  (1)..(1108)
<223>  BTCas12b.BTCas12b polypeptide sequence

<400>  271

Met Ala Thr Arg Ser Phe Ile Leu Lys Ile Glu Pro Asn Glu Glu Val 
1               5                   10                  15      


Lys Lys Gly Leu Trp Lys Thr His Glu Val Leu Asn His Gly Ile Ala 
            20                  25                  30          


Tyr Tyr Met Asn Ile Leu Lys Leu Ile Arg Gln Glu Ala Ile Tyr Glu 
        35                  40                  45              


His His Glu Gln Asp Pro Lys Asn Pro Lys Lys Val Ser Lys Ala Glu 
    50                  55                  60                  


Ile Gln Ala Glu Leu Trp Asp Phe Val Leu Lys Met Gln Lys Cys Asn 
65                  70                  75                  80  


Ser Phe Thr His Glu Val Asp Lys Asp Val Val Phe Asn Ile Leu Arg 
                85                  90                  95      


Glu Leu Tyr Glu Glu Leu Val Pro Ser Ser Val Glu Lys Lys Gly Glu 
            100                 105                 110         


Ala Asn Gln Leu Ser Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn 
        115                 120                 125             


Ser Gln Ser Gly Lys Gly Thr Ala Ser Ser Gly Arg Lys Pro Arg Trp 
    130                 135                 140                 


Tyr Asn Leu Lys Ile Ala Gly Asp Pro Ser Trp Glu Glu Glu Lys Lys 
145                 150                 155                 160 


Lys Trp Glu Glu Asp Lys Lys Lys Asp Pro Leu Ala Lys Ile Leu Gly 
                165                 170                 175     


Lys Leu Ala Glu Tyr Gly Leu Ile Pro Leu Phe Ile Pro Phe Thr Asp 
            180                 185                 190         


Ser Asn Glu Pro Ile Val Lys Glu Ile Lys Trp Met Glu Lys Ser Arg 
        195                 200                 205             


Asn Gln Ser Val Arg Arg Leu Asp Lys Asp Met Phe Ile Gln Ala Leu 
    210                 215                 220                 


Glu Arg Phe Leu Ser Trp Glu Ser Trp Asn Leu Lys Val Lys Glu Glu 
225                 230                 235                 240 


Tyr Glu Lys Val Glu Lys Glu His Lys Thr Leu Glu Glu Arg Ile Lys 
                245                 250                 255     


Glu Asp Ile Gln Ala Phe Lys Ser Leu Glu Gln Tyr Glu Lys Glu Arg 
            260                 265                 270         


Gln Glu Gln Leu Leu Arg Asp Thr Leu Asn Thr Asn Glu Tyr Arg Leu 
        275                 280                 285             


Ser Lys Arg Gly Leu Arg Gly Trp Arg Glu Ile Ile Gln Lys Trp Leu 
    290                 295                 300                 


Lys Met Asp Glu Asn Glu Pro Ser Glu Lys Tyr Leu Glu Val Phe Lys 
305                 310                 315                 320 


Asp Tyr Gln Arg Lys His Pro Arg Glu Ala Gly Asp Tyr Ser Val Tyr 
                325                 330                 335     


Glu Phe Leu Ser Lys Lys Glu Asn His Phe Ile Trp Arg Asn His Pro 
            340                 345                 350         


Glu Tyr Pro Tyr Leu Tyr Ala Thr Phe Cys Glu Ile Asp Lys Lys Lys 
        355                 360                 365             


Lys Asp Ala Lys Gln Gln Ala Thr Phe Thr Leu Ala Asp Pro Ile Asn 
    370                 375                 380                 


His Pro Leu Trp Val Arg Phe Glu Glu Arg Ser Gly Ser Asn Leu Asn 
385                 390                 395                 400 


Lys Tyr Arg Ile Leu Thr Glu Gln Leu His Thr Glu Lys Leu Lys Lys 
                405                 410                 415     


Lys Leu Thr Val Gln Leu Asp Arg Leu Ile Tyr Pro Thr Glu Ser Gly 
            420                 425                 430         


Gly Trp Glu Glu Lys Gly Lys Val Asp Ile Val Leu Leu Pro Ser Arg 
        435                 440                 445             


Gln Phe Tyr Asn Gln Ile Phe Leu Asp Ile Glu Glu Lys Gly Lys His 
    450                 455                 460                 


Ala Phe Thr Tyr Lys Asp Glu Ser Ile Lys Phe Pro Leu Lys Gly Thr 
465                 470                 475                 480 


Leu Gly Gly Ala Arg Val Gln Phe Asp Arg Asp His Leu Arg Arg Tyr 
                485                 490                 495     


Pro His Lys Val Glu Ser Gly Asn Val Gly Arg Ile Tyr Phe Asn Met 
            500                 505                 510         


Thr Val Asn Ile Glu Pro Thr Glu Ser Pro Val Ser Lys Ser Leu Lys 
        515                 520                 525             


Ile His Arg Asp Asp Phe Pro Lys Phe Val Asn Phe Lys Pro Lys Glu 
    530                 535                 540                 


Leu Thr Glu Trp Ile Lys Asp Ser Lys Gly Lys Lys Leu Lys Ser Gly 
545                 550                 555                 560 


Ile Glu Ser Leu Glu Ile Gly Leu Arg Val Met Ser Ile Asp Leu Gly 
                565                 570                 575     


Gln Arg Gln Ala Ala Ala Ala Ser Ile Phe Glu Val Val Asp Gln Lys 
            580                 585                 590         


Pro Asp Ile Glu Gly Lys Leu Phe Phe Pro Ile Lys Gly Thr Glu Leu 
        595                 600                 605             


Tyr Ala Val His Arg Ala Ser Phe Asn Ile Lys Leu Pro Gly Glu Thr 
    610                 615                 620                 


Leu Val Lys Ser Arg Glu Val Leu Arg Lys Ala Arg Glu Asp Asn Leu 
625                 630                 635                 640 


Lys Leu Met Asn Gln Lys Leu Asn Phe Leu Arg Asn Val Leu His Phe 
                645                 650                 655     


Gln Gln Phe Glu Asp Ile Thr Glu Arg Glu Lys Arg Val Thr Lys Trp 
            660                 665                 670         


Ile Ser Arg Gln Glu Asn Ser Asp Val Pro Leu Val Tyr Gln Asp Glu 
        675                 680                 685             


Leu Ile Gln Ile Arg Glu Leu Met Tyr Lys Pro Tyr Lys Asp Trp Val 
    690                 695                 700                 


Ala Phe Leu Lys Gln Leu His Lys Arg Leu Glu Val Glu Ile Gly Lys 
705                 710                 715                 720 


Glu Val Lys His Trp Arg Lys Ser Leu Ser Asp Gly Arg Lys Gly Leu 
                725                 730                 735     


Tyr Gly Ile Ser Leu Lys Asn Ile Asp Glu Ile Asp Arg Thr Arg Lys 
            740                 745                 750         


Phe Leu Leu Arg Trp Ser Leu Arg Pro Thr Glu Pro Gly Glu Val Arg 
        755                 760                 765             


Arg Leu Glu Pro Gly Gln Arg Phe Ala Ile Asp Gln Leu Asn His Leu 
    770                 775                 780                 


Asn Ala Leu Lys Glu Asp Arg Leu Lys Lys Met Ala Asn Thr Ile Ile 
785                 790                 795                 800 


Met His Ala Leu Gly Tyr Cys Tyr Asp Val Arg Lys Lys Lys Trp Gln 
                805                 810                 815     


Ala Lys Asn Pro Ala Cys Gln Ile Ile Leu Phe Glu Asp Leu Ser Asn 
            820                 825                 830         


Tyr Asn Pro Tyr Glu Glu Arg Ser Arg Phe Glu Asn Ser Lys Leu Met 
        835                 840                 845             


Lys Trp Ser Arg Arg Glu Ile Pro Arg Gln Val Ala Leu Gln Gly Glu 
    850                 855                 860                 


Ile Tyr Gly Leu Gln Val Gly Glu Val Gly Ala Gln Phe Ser Ser Arg 
865                 870                 875                 880 


Phe His Ala Lys Thr Gly Ser Pro Gly Ile Arg Cys Ser Val Val Thr 
                885                 890                 895     


Lys Glu Lys Leu Gln Asp Asn Arg Phe Phe Lys Asn Leu Gln Arg Glu 
            900                 905                 910         


Gly Arg Leu Thr Leu Asp Lys Ile Ala Val Leu Lys Glu Gly Asp Leu 
        915                 920                 925             


Tyr Pro Asp Lys Gly Gly Glu Lys Phe Ile Ser Leu Ser Lys Asp Arg 
    930                 935                 940                 


Lys Leu Val Thr Thr His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln 
945                 950                 955                 960 


Lys Arg Phe Trp Thr Arg Thr His Gly Phe Tyr Lys Val Tyr Cys Lys 
                965                 970                 975     


Ala Tyr Gln Val Asp Gly Gln Thr Val Tyr Ile Pro Glu Ser Lys Asp 
            980                 985                 990         


Gln Lys Gln Lys Ile Ile Glu Glu  Phe Gly Glu Gly Tyr  Phe Ile Leu 
        995                 1000                 1005             


Lys Asp  Gly Val Tyr Glu Trp  Gly Asn Ala Gly Lys  Leu Lys Ile 
    1010                 1015                 1020             


Lys Lys  Gly Ser Ser Lys Gln  Ser Ser Ser Glu Leu  Val Asp Ser 
    1025                 1030                 1035             


Asp Ile  Leu Lys Asp Ser Phe  Asp Leu Ala Ser Glu  Leu Lys Gly 
    1040                 1045                 1050             


Glu Lys  Leu Met Leu Tyr Arg  Asp Pro Ser Gly Asn  Val Phe Pro 
    1055                 1060                 1065             


Ser Asp  Lys Trp Met Ala Ala  Gly Val Phe Phe Gly  Lys Leu Glu 
    1070                 1075                 1080             


Arg Ile  Leu Ile Ser Lys Leu  Thr Asn Gln Tyr Ser  Ile Ser Thr 
    1085                 1090                 1095             


Ile Glu  Asp Asp Ser Ser Lys  Gln Ser Met 
    1100                 1105             


<210>  272
<211>  1112
<212>  PRT
<213>  Bacillus sp. V3-13


<220>
<221>  MISC_FEATURE
<222>  (1)..(1112)
<223>  BvCas12b (Bacillus sp. V3-13) polypeptide sequence

<400>  272

Met Ala Ile Arg Ser Ile Lys Leu Lys Met Lys Thr Asn Ser Gly Thr 
1               5                   10                  15      


Asp Ser Ile Tyr Leu Arg Lys Ala Leu Trp Arg Thr His Gln Leu Ile 
            20                  25                  30          


Asn Glu Gly Ile Ala Tyr Tyr Met Asn Leu Leu Thr Leu Tyr Arg Gln 
        35                  40                  45              


Glu Ala Ile Gly Asp Lys Thr Lys Glu Ala Tyr Gln Ala Glu Leu Ile 
    50                  55                  60                  


Asn Ile Ile Arg Asn Gln Gln Arg Asn Asn Gly Ser Ser Glu Glu His 
65                  70                  75                  80  


Gly Ser Asp Gln Glu Ile Leu Ala Leu Leu Arg Gln Leu Tyr Glu Leu 
                85                  90                  95      


Ile Ile Pro Ser Ser Ile Gly Glu Ser Gly Asp Ala Asn Gln Leu Gly 
            100                 105                 110         


Asn Lys Phe Leu Tyr Pro Leu Val Asp Pro Asn Ser Gln Ser Gly Lys 
        115                 120                 125             


Gly Thr Ser Asn Ala Gly Arg Lys Pro Arg Trp Lys Arg Leu Lys Glu 
    130                 135                 140                 


Glu Gly Asn Pro Asp Trp Glu Leu Glu Lys Lys Lys Asp Glu Glu Arg 
145                 150                 155                 160 


Lys Ala Lys Asp Pro Thr Val Lys Ile Phe Asp Asn Leu Asn Lys Tyr 
                165                 170                 175     


Gly Leu Leu Pro Leu Phe Pro Leu Phe Thr Asn Ile Gln Lys Asp Ile 
            180                 185                 190         


Glu Trp Leu Pro Leu Gly Lys Arg Gln Ser Val Arg Lys Trp Asp Lys 
        195                 200                 205             


Asp Met Phe Ile Gln Ala Ile Glu Arg Leu Leu Ser Trp Glu Ser Trp 
    210                 215                 220                 


Asn Arg Arg Val Ala Asp Glu Tyr Lys Gln Leu Lys Glu Lys Thr Glu 
225                 230                 235                 240 


Ser Tyr Tyr Lys Glu His Leu Thr Gly Gly Glu Glu Trp Ile Glu Lys 
                245                 250                 255     


Ile Arg Lys Phe Glu Lys Glu Arg Asn Met Glu Leu Glu Lys Asn Ala 
            260                 265                 270         


Phe Ala Pro Asn Asp Gly Tyr Phe Ile Thr Ser Arg Gln Ile Arg Gly 
        275                 280                 285             


Trp Asp Arg Val Tyr Glu Lys Trp Ser Lys Leu Pro Glu Ser Ala Ser 
    290                 295                 300                 


Pro Glu Glu Leu Trp Lys Val Val Ala Glu Gln Gln Asn Lys Met Ser 
305                 310                 315                 320 


Glu Gly Phe Gly Asp Pro Lys Val Phe Ser Phe Leu Ala Asn Arg Glu 
                325                 330                 335     


Asn Arg Asp Ile Trp Arg Gly His Ser Glu Arg Ile Tyr His Ile Ala 
            340                 345                 350         


Ala Tyr Asn Gly Leu Gln Lys Lys Leu Ser Arg Thr Lys Glu Gln Ala 
        355                 360                 365             


Thr Phe Thr Leu Pro Asp Ala Ile Glu His Pro Leu Trp Ile Arg Tyr 
    370                 375                 380                 


Glu Ser Pro Gly Gly Thr Asn Leu Asn Leu Phe Lys Leu Glu Glu Lys 
385                 390                 395                 400 


Gln Lys Lys Asn Tyr Tyr Val Thr Leu Ser Lys Ile Ile Trp Pro Ser 
                405                 410                 415     


Glu Glu Lys Trp Ile Glu Lys Glu Asn Ile Glu Ile Pro Leu Ala Pro 
            420                 425                 430         


Ser Ile Gln Phe Asn Arg Gln Ile Lys Leu Lys Gln His Val Lys Gly 
        435                 440                 445             


Lys Gln Glu Ile Ser Phe Ser Asp Tyr Ser Ser Arg Ile Ser Leu Asp 
    450                 455                 460                 


Gly Val Leu Gly Gly Ser Arg Ile Gln Phe Asn Arg Lys Tyr Ile Lys 
465                 470                 475                 480 


Asn His Lys Glu Leu Leu Gly Glu Gly Asp Ile Gly Pro Val Phe Phe 
                485                 490                 495     


Asn Leu Val Val Asp Val Ala Pro Leu Gln Glu Thr Arg Asn Gly Arg 
            500                 505                 510         


Leu Gln Ser Pro Ile Gly Lys Ala Leu Lys Val Ile Ser Ser Asp Phe 
        515                 520                 525             


Ser Lys Val Ile Asp Tyr Lys Pro Lys Glu Leu Met Asp Trp Met Asn 
    530                 535                 540                 


Thr Gly Ser Ala Ser Asn Ser Phe Gly Val Ala Ser Leu Leu Glu Gly 
545                 550                 555                 560 


Met Arg Val Met Ser Ile Asp Met Gly Gln Arg Thr Ser Ala Ser Val 
                565                 570                 575     


Ser Ile Phe Glu Val Val Lys Glu Leu Pro Lys Asp Gln Glu Gln Lys 
            580                 585                 590         


Leu Phe Tyr Ser Ile Asn Asp Thr Glu Leu Phe Ala Ile His Lys Arg 
        595                 600                 605             


Ser Phe Leu Leu Asn Leu Pro Gly Glu Val Val Thr Lys Asn Asn Lys 
    610                 615                 620                 


Gln Gln Arg Gln Glu Arg Arg Lys Lys Arg Gln Phe Val Arg Ser Gln 
625                 630                 635                 640 


Ile Arg Met Leu Ala Asn Val Leu Arg Leu Glu Thr Lys Lys Thr Pro 
                645                 650                 655     


Asp Glu Arg Lys Lys Ala Ile His Lys Leu Met Glu Ile Val Gln Ser 
            660                 665                 670         


Tyr Asp Ser Trp Thr Ala Ser Gln Lys Glu Val Trp Glu Lys Glu Leu 
        675                 680                 685             


Asn Leu Leu Thr Asn Met Ala Ala Phe Asn Asp Glu Ile Trp Lys Glu 
    690                 695                 700                 


Ser Leu Val Glu Leu His His Arg Ile Glu Pro Tyr Val Gly Gln Ile 
705                 710                 715                 720 


Val Ser Lys Trp Arg Lys Gly Leu Ser Glu Gly Arg Lys Asn Leu Ala 
                725                 730                 735     


Gly Ile Ser Met Trp Asn Ile Asp Glu Leu Glu Asp Thr Arg Arg Leu 
            740                 745                 750         


Leu Ile Ser Trp Ser Lys Arg Ser Arg Thr Pro Gly Glu Ala Asn Arg 
        755                 760                 765             


Ile Glu Thr Asp Glu Pro Phe Gly Ser Ser Leu Leu Gln His Ile Gln 
    770                 775                 780                 


Asn Val Lys Asp Asp Arg Leu Lys Gln Met Ala Asn Leu Ile Ile Met 
785                 790                 795                 800 


Thr Ala Leu Gly Phe Lys Tyr Asp Lys Glu Glu Lys Asp Arg Tyr Lys 
                805                 810                 815     


Arg Trp Lys Glu Thr Tyr Pro Ala Cys Gln Ile Ile Leu Phe Glu Asn 
            820                 825                 830         


Leu Asn Arg Tyr Leu Phe Asn Leu Asp Arg Ser Arg Arg Glu Asn Ser 
        835                 840                 845             


Arg Leu Met Lys Trp Ala His Arg Ser Ile Pro Arg Thr Val Ser Met 
    850                 855                 860                 


Gln Gly Glu Met Phe Gly Leu Gln Val Gly Asp Val Arg Ser Glu Tyr 
865                 870                 875                 880 


Ser Ser Arg Phe His Ala Lys Thr Gly Ala Pro Gly Ile Arg Cys His 
                885                 890                 895     


Ala Leu Thr Glu Glu Asp Leu Lys Ala Gly Ser Asn Thr Leu Lys Arg 
            900                 905                 910         


Leu Ile Glu Asp Gly Phe Ile Asn Glu Ser Glu Leu Ala Tyr Leu Lys 
        915                 920                 925             


Lys Gly Asp Ile Ile Pro Ser Gln Gly Gly Glu Leu Phe Val Thr Leu 
    930                 935                 940                 


Ser Lys Arg Tyr Lys Lys Asp Ser Asp Asn Asn Glu Leu Thr Val Ile 
945                 950                 955                 960 


His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln Lys Arg Phe Trp Gln 
                965                 970                 975     


Gln Asn Ser Glu Val Tyr Arg Val Pro Cys Gln Leu Ala Arg Met Gly 
            980                 985                 990         


Glu Asp Lys Leu Tyr Ile Pro Lys  Ser Gln Thr Glu Thr  Ile Lys Lys 
        995                 1000                 1005             


Tyr Phe  Gly Lys Gly Ser Phe  Val Lys Asn Asn Thr  Glu Gln Glu 
    1010                 1015                 1020             


Val Tyr  Lys Trp Glu Lys Ser  Glu Lys Met Lys Ile  Lys Thr Asp 
    1025                 1030                 1035             


Thr Thr  Phe Asp Leu Gln Asp  Leu Asp Gly Phe Glu  Asp Ile Ser 
    1040                 1045                 1050             


Lys Thr  Ile Glu Leu Ala Gln  Glu Gln Gln Lys Lys  Tyr Leu Thr 
    1055                 1060                 1065             


Met Phe  Arg Asp Pro Ser Gly  Tyr Phe Phe Asn Asn  Glu Thr Trp 
    1070                 1075                 1080             


Arg Pro  Gln Lys Glu Tyr Trp  Ser Ile Val Asn Asn  Ile Ile Lys 
    1085                 1090                 1095             


Ser Cys  Leu Lys Lys Lys Ile  Leu Ser Asn Lys Val  Glu Leu 
    1100                 1105                 1110         


<210>  273
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 5'UTR oligonucleotide 
       sequence

<400>  273
gggaaataag agagaaaaga agagtaagaa gaaatataag agccacc                     47


<210>  274
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 3'UTR (TriLink standard UTR) 
       polynucleotide sequence

<400>  274
gctggagcct cggtggccat gcttcttgcc ccttgggcct ccccccagcc cctcctcccc       60

ttcctgcacc cgtacccccg tggtctttga ataaagtctg a                          101


<210>  275
<211>  3420
<212>  DNA
<213>  Bacillus hisashii


<220>
<221>  misc_feature
<222>  (1)..(3420)
<223>  bhCas12b (V4) polynucleotide sequence

<400>  275
atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgccaccaga       60

tccttcatcc tgaagatcga gcccaacgag gaagtgaaga aaggcctctg gaaaacccac      120

gaggtgctga accacggaat cgcctactac atgaatatcc tgaagctgat ccggcaagag      180

gccatctacg agcaccacga gcaggacccc aagaatccca agaaggtgtc caaggccgag      240

atccaggccg agctgtggga tttcgtgctg aagatgcaga agtgcaacag cttcacacac      300

gaggtggaca aggacgaggt gttcaacatc ctgagagagc tgtacgagga actggtgccc      360

agcagcgtgg aaaagaaggg cgaagccaac cagctgagca acaagtttct gtaccctctg      420

gtggacccca acagccagtc tggaaaggga acagccagca gcggcagaaa gcccagatgg      480

tacaacctga agattgccgg cgatccctcc tgggaagaag agaagaagaa gtgggaagaa      540

gataagaaaa aggacccgct ggccaagatc ctgggcaagc tggctgagta cggactgatc      600

cctctgttca tcccctacac cgacagcaac gagcccatcg tgaaagaaat caagtggatg      660

gaaaagtccc ggaaccagag cgtgcggcgg ctggataagg acatgttcat tcaggccctg      720

gaacggttcc tgagctggga gagctggaac ctgaaagtga aagaggaata cgagaaggtc      780

gagaaagagt acaagaccct ggaagagagg atcaaagagg acatccaggc tctgaaggct      840

ctggaacagt atgagaaaga gcggcaagaa cagctgctgc gggacaccct gaacaccaac      900

gagtaccggc tgagcaagag aggccttaga ggctggcggg aaatcatcca gaaatggctg      960

aaaatggacg agaacgagcc ctccgagaag tacctggaag tgttcaagga ctaccagcgg     1020

aagcacccta gagaggccgg cgattacagc gtgtacgagt tcctgtccaa gaaagagaac     1080

cacttcatct ggcggaatca ccctgagtac ccctacctgt acgccacctt ctgcgagatc     1140

gacaagaaaa agaaggacgc caagcagcag gccaccttca cactggccga tcctatcaat     1200

caccctctgt gggtccgatt cgaggaaaga agcggcagca acctgaacaa gtacagaatc     1260

ctgaccgagc agctgcacac cgagaagctg aagaaaaagc tgacagtgca gctggaccgg     1320

ctgatctacc ctacagaatc tggcggctgg gaagagaagg gcaaagtgga cattgtgctg     1380

ctgcccagcc ggcagttcta caaccagatc ttcctggaca tcgaggaaaa gggcaagcac     1440

gccttcacct acaaggatga gagcatcaag ttccctctga agggcacact cggcggagcc     1500

agagtgcagt tcgacagaga tcacctgaga agataccctc acaaggtgga aagcggcaac     1560

gtgggcagaa tctacttcaa catgaccgtg aacatcgagc ctacagagtc cccagtgtcc     1620

aagtctctga agatccaccg ggacgacttc cccaaggtgg tcaacttcaa gcccaaagaa     1680

ctgaccgagt ggatcaagga cagcaagggc aagaaactga agtccggcat cgagtccctg     1740

gaaatcggcc tgagagtgat gagcatcgac ctgggacaga gacaggccgc tgccgcctct     1800

attttcgagg tggtggatca gaagcccgac atcgaaggca agctgttttt cccaatcaag     1860

ggcaccgagc tgtatgccgt gcacagagcc agcttcaaca tcaagctgcc cggcgagaca     1920

ctggtcaaga gcagagaagt gctgcggaag gccagagagg acaatctgaa actgatgaac     1980

cagaagctca acttcctgcg gaacgtgctg cacttccagc agttcgagga catcaccgag     2040

agagagaagc gggtcaccaa gtggatcagc agacaagaga acagcgacgt gcccctggtg     2100

taccaggatg agctgatcca gatccgcgag ctgatgtaca agccttacaa ggactgggtc     2160

gccttcctga agcagctcca caagagactg gaagtcgaga tcggcaaaga agtgaagcac     2220

tggcggaagt ccctgagcga cggaagaaag ggcctgtacg gcatctccct gaagaacatc     2280

gacgagatcg atcggacccg gaagttcctg ctgagatggt ccctgaggcc taccgaacct     2340

ggcgaagtgc gtagactgga acccggccag agattcgcca tcgaccagct gaatcacctg     2400

aacgccctga aagaagatcg gctgaagaag atggccaaca ccatcatcat gcacgccctg     2460

ggctactgct acgacgtgcg gaagaagaaa tggcaggcta agaaccccgc ctgccagatc     2520

atcctgttcg aggatctgag caactacaac ccctacgagg aaaggtcccg cttcgagaac     2580

agcaagctca tgaagtggtc cagacgcgag atccccagac aggttgcact gcagggcgag     2640

atctatggcc tgcaagtggg agaagtgggc gctcagttca gcagcagatt ccacgccaag     2700

acaggcagcc ctggcatcag atgtagcgtc gtgaccaaag agaagctgca ggacaatcgg     2760

ttcttcaaga atctgcagag agagggcaga ctgaccctgg acaaaatcgc cgtgctgaaa     2820

gagggcgatc tgtacccaga caaaggcggc gagaagttca tcagcctgag caaggatcgg     2880

aagtgcgtga ccacacacgc cgacatcaac gccgctcaga acctgcagaa gcggttctgg     2940

acaagaaccc acggcttcta caaggtgtac tgcaaggcct accaggtgga cggccagacc     3000

gtgtacatcc ctgagagcaa ggaccagaag cagaagatca tcgaagagtt cggcgagggc     3060

tacttcattc tgaaggacgg ggtgtacgaa tgggtcaacg ccggcaagct gaaaatcaag     3120

aagggcagct ccaagcagag cagcagcgag ctggtggata gcgacatcct gaaagacagc     3180

ttcgacctgg cctccgagct gaaaggcgaa aagctgatgc tgtacaggga ccccagcggc     3240

aatgtgttcc ccagcgacaa atggatggcc gctggcgtgt tcttcggaaa gctggaacgc     3300

atcctgatca gcaagctgac caaccagtac tccatcagca ccatcgagga cgacagcagc     3360

aagcagtcta tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag     3420


<210>  276
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  276

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala 
    


<210>  277
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  277
atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc c                51


<210>  278
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 101 Cas9 TadAins 1015 
       polypeptide sequence

<400>  278

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Gly Ser Ser Gly Ser  Glu Thr Pro 
    1010                 1015                 1020             


Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser Ser Gly  Ser Glu Val 
    1025                 1030                 1035             


Glu Phe  Ser His Glu Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala 
    1040                 1045                 1050             


Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro Val Gly  Ala Val Leu 
    1055                 1060                 1065             


Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile 
    1070                 1075                 1080             


Gly Leu  His Asp Pro Thr Ala  His Ala Glu Ile Met  Ala Leu Arg 
    1085                 1090                 1095             


Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg Leu Ile  Asp Ala Thr 
    1100                 1105                 1110             


Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met Cys Ala  Gly Ala Met 
    1115                 1120                 1125             


Ile His  Ser Arg Ile Gly Arg  Val Val Phe Gly Val  Arg Asn Ala 
    1130                 1135                 1140             


Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp Val Leu  His Tyr Pro 
    1145                 1150                 1155             


Gly Met  Asn His Arg Val Glu  Ile Thr Glu Gly Ile  Leu Ala Asp 
    1160                 1165                 1170             


Glu Cys  Ala Ala Leu Leu Cys  Tyr Phe Phe Arg Met  Pro Arg Gln 
    1175                 1180                 1185             


Val Phe  Asn Ala Gln Lys Lys  Ala Gln Ser Ser Thr  Asp Tyr Asp 
    1190                 1195                 1200             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  279
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 102 Cas9 TadAins 1022 
       polypeptide sequence

<400>  279

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Gly 
    1010                 1015                 1020             


Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro 
    1025                 1030                 1035             


Glu Ser  Ser Gly Ser Glu Val  Glu Phe Ser His Glu  Tyr Trp Met 
    1040                 1045                 1050             


Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu 
    1055                 1060                 1065             


Val Pro  Val Gly Ala Val Leu  Val Leu Asn Asn Arg  Val Ile Gly 
    1070                 1075                 1080             


Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His Asp Pro  Thr Ala His 
    1085                 1090                 1095             


Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn 
    1100                 1105                 1110             


Tyr Arg  Leu Ile Asp Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys 
    1115                 1120                 1125             


Val Met  Cys Ala Gly Ala Met  Ile His Ser Arg Ile  Gly Arg Val 
    1130                 1135                 1140             


Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu 
    1145                 1150                 1155             


Met Asp  Val Leu His Tyr Pro  Gly Met Asn His Arg  Val Glu Ile 
    1160                 1165                 1170             


Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr 
    1175                 1180                 1185             


Phe Phe  Arg Met Pro Arg Gln  Val Phe Asn Ala Gln  Lys Lys Ala 
    1190                 1195                 1200             


Gln Ser  Ser Thr Asp Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  280
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 103 Cas9 TadAins 1029 
       polypeptide sequence

<400>  280

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Ser Ser Gly Ser Glu  Thr Pro Gly 
    1025                 1030                 1035             


Thr Ser  Glu Ser Ala Thr Pro  Glu Ser Ser Gly Ser  Glu Val Glu 
    1040                 1045                 1050             


Phe Ser  His Glu Tyr Trp Met  Arg His Ala Leu Thr  Leu Ala Lys 
    1055                 1060                 1065             


Arg Ala  Arg Asp Glu Arg Glu  Val Pro Val Gly Ala  Val Leu Val 
    1070                 1075                 1080             


Leu Asn  Asn Arg Val Ile Gly  Glu Gly Trp Asn Arg  Ala Ile Gly 
    1085                 1090                 1095             


Leu His  Asp Pro Thr Ala His  Ala Glu Ile Met Ala  Leu Arg Gln 
    1100                 1105                 1110             


Gly Gly  Leu Val Met Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu 
    1115                 1120                 1125             


Tyr Val  Thr Phe Glu Pro Cys  Val Met Cys Ala Gly  Ala Met Ile 
    1130                 1135                 1140             


His Ser  Arg Ile Gly Arg Val  Val Phe Gly Val Arg  Asn Ala Lys 
    1145                 1150                 1155             


Thr Gly  Ala Ala Gly Ser Leu  Met Asp Val Leu His  Tyr Pro Gly 
    1160                 1165                 1170             


Met Asn  His Arg Val Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu 
    1175                 1180                 1185             


Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val 
    1190                 1195                 1200             


Phe Asn  Ala Gln Lys Lys Ala  Gln Ser Ser Thr Asp  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  281
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 103 Cas9 TadAins 1040 
       polypeptide sequence

<400>  281

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Gly Ser Ser Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser 
    1040                 1045                 1050             


Ala Thr  Pro Glu Ser Ser Gly  Ser Glu Val Glu Phe  Ser His Glu 
    1055                 1060                 1065             


Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala Lys Arg  Ala Arg Asp 
    1070                 1075                 1080             


Glu Arg  Glu Val Pro Val Gly  Ala Val Leu Val Leu  Asn Asn Arg 
    1085                 1090                 1095             


Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile Gly Leu  His Asp Pro 
    1100                 1105                 1110             


Thr Ala  His Ala Glu Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val 
    1115                 1120                 1125             


Met Gln  Asn Tyr Arg Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe 
    1130                 1135                 1140             


Glu Pro  Cys Val Met Cys Ala  Gly Ala Met Ile His  Ser Arg Ile 
    1145                 1150                 1155             


Gly Arg  Val Val Phe Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala 
    1160                 1165                 1170             


Gly Ser  Leu Met Asp Val Leu  His Tyr Pro Gly Met  Asn His Arg 
    1175                 1180                 1185             


Val Glu  Ile Thr Glu Gly Ile  Leu Ala Asp Glu Cys  Ala Ala Leu 
    1190                 1195                 1200             


Leu Cys  Tyr Phe Phe Arg Met  Pro Arg Gln Val Phe  Asn Ala Gln 
    1205                 1210                 1215             


Lys Lys  Ala Gln Ser Ser Thr  Asp Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  282
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 105 Cas9 TadAins 1068 
       polypeptide sequence

<400>  282

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr 
    1070                 1075                 1080             


Pro Glu  Ser Ser Gly Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp 
    1085                 1090                 1095             


Met Arg  His Ala Leu Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg 
    1100                 1105                 1110             


Glu Val  Pro Val Gly Ala Val  Leu Val Leu Asn Asn  Arg Val Ile 
    1115                 1120                 1125             


Gly Glu  Gly Trp Asn Arg Ala  Ile Gly Leu His Asp  Pro Thr Ala 
    1130                 1135                 1140             


His Ala  Glu Ile Met Ala Leu  Arg Gln Gly Gly Leu  Val Met Gln 
    1145                 1150                 1155             


Asn Tyr  Arg Leu Ile Asp Ala  Thr Leu Tyr Val Thr  Phe Glu Pro 
    1160                 1165                 1170             


Cys Val  Met Cys Ala Gly Ala  Met Ile His Ser Arg  Ile Gly Arg 
    1175                 1180                 1185             


Val Val  Phe Gly Val Arg Asn  Ala Lys Thr Gly Ala  Ala Gly Ser 
    1190                 1195                 1200             


Leu Met  Asp Val Leu His Tyr  Pro Gly Met Asn His  Arg Val Glu 
    1205                 1210                 1215             


Ile Thr  Glu Gly Ile Leu Ala  Asp Glu Cys Ala Ala  Leu Leu Cys 
    1220                 1225                 1230             


Tyr Phe  Phe Arg Met Pro Arg  Gln Val Phe Asn Ala  Gln Lys Lys 
    1235                 1240                 1245             


Ala Gln  Ser Ser Thr Asp Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  283
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 106 Cas9 TadAins 1247 
       polypeptide sequence

<400>  283

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Gly 
    1235                 1240                 1245             


Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro 
    1250                 1255                 1260             


Glu Ser  Ser Gly Ser Glu Val  Glu Phe Ser His Glu  Tyr Trp Met 
    1265                 1270                 1275             


Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu 
    1280                 1285                 1290             


Val Pro  Val Gly Ala Val Leu  Val Leu Asn Asn Arg  Val Ile Gly 
    1295                 1300                 1305             


Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His Asp Pro  Thr Ala His 
    1310                 1315                 1320             


Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn 
    1325                 1330                 1335             


Tyr Arg  Leu Ile Asp Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys 
    1340                 1345                 1350             


Val Met  Cys Ala Gly Ala Met  Ile His Ser Arg Ile  Gly Arg Val 
    1355                 1360                 1365             


Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu 
    1370                 1375                 1380             


Met Asp  Val Leu His Tyr Pro  Gly Met Asn His Arg  Val Glu Ile 
    1385                 1390                 1395             


Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr 
    1400                 1405                 1410             


Phe Phe  Arg Met Pro Arg Gln  Val Phe Asn Ala Gln  Lys Lys Ala 
    1415                 1420                 1425             


Gln Ser  Ser Thr Asp Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  284
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 107 Cas9 TadAins 1054 
       polypeptide sequence

<400>  284

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Ser Ser Gly Ser Glu  Thr Pro Gly Thr Ser  Glu Ser Ala 
    1055                 1060                 1065             


Thr Pro  Glu Ser Ser Gly Ser  Glu Val Glu Phe Ser  His Glu Tyr 
    1070                 1075                 1080             


Trp Met  Arg His Ala Leu Thr  Leu Ala Lys Arg Ala  Arg Asp Glu 
    1085                 1090                 1095             


Arg Glu  Val Pro Val Gly Ala  Val Leu Val Leu Asn  Asn Arg Val 
    1100                 1105                 1110             


Ile Gly  Glu Gly Trp Asn Arg  Ala Ile Gly Leu His  Asp Pro Thr 
    1115                 1120                 1125             


Ala His  Ala Glu Ile Met Ala  Leu Arg Gln Gly Gly  Leu Val Met 
    1130                 1135                 1140             


Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu Tyr Val  Thr Phe Glu 
    1145                 1150                 1155             


Pro Cys  Val Met Cys Ala Gly  Ala Met Ile His Ser  Arg Ile Gly 
    1160                 1165                 1170             


Arg Val  Val Phe Gly Val Arg  Asn Ala Lys Thr Gly  Ala Ala Gly 
    1175                 1180                 1185             


Ser Leu  Met Asp Val Leu His  Tyr Pro Gly Met Asn  His Arg Val 
    1190                 1195                 1200             


Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu 
    1205                 1210                 1215             


Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val Phe Asn  Ala Gln Lys 
    1220                 1225                 1230             


Lys Ala  Gln Ser Ser Thr Asp  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  285
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 108 Cas9 TadAins 1026 
       polypeptide sequence

<400>  285

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gly Ser Ser Gly  Ser Glu Thr Pro Gly  Thr Ser Glu 
    1025                 1030                 1035             


Ser Ala  Thr Pro Glu Ser Ser  Gly Ser Glu Val Glu  Phe Ser His 
    1040                 1045                 1050             


Glu Tyr  Trp Met Arg His Ala  Leu Thr Leu Ala Lys  Arg Ala Arg 
    1055                 1060                 1065             


Asp Glu  Arg Glu Val Pro Val  Gly Ala Val Leu Val  Leu Asn Asn 
    1070                 1075                 1080             


Arg Val  Ile Gly Glu Gly Trp  Asn Arg Ala Ile Gly  Leu His Asp 
    1085                 1090                 1095             


Pro Thr  Ala His Ala Glu Ile  Met Ala Leu Arg Gln  Gly Gly Leu 
    1100                 1105                 1110             


Val Met  Gln Asn Tyr Arg Leu  Ile Asp Ala Thr Leu  Tyr Val Thr 
    1115                 1120                 1125             


Phe Glu  Pro Cys Val Met Cys  Ala Gly Ala Met Ile  His Ser Arg 
    1130                 1135                 1140             


Ile Gly  Arg Val Val Phe Gly  Val Arg Asn Ala Lys  Thr Gly Ala 
    1145                 1150                 1155             


Ala Gly  Ser Leu Met Asp Val  Leu His Tyr Pro Gly  Met Asn His 
    1160                 1165                 1170             


Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala Asp Glu  Cys Ala Ala 
    1175                 1180                 1185             


Leu Leu  Cys Tyr Phe Phe Arg  Met Pro Arg Gln Val  Phe Asn Ala 
    1190                 1195                 1200             


Gln Lys  Lys Ala Gln Ser Ser  Thr Asp Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  286
<211>  1540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 109 Cas9 TadAins 768 
       polypeptide sequence

<400>  286

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
    770                 775                 780                 


Glu Ser Ser Gly Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg 
785                 790                 795                 800 


His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro 
                805                 810                 815     


Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp 
            820                 825                 830         


Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met 
        835                 840                 845             


Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp 
    850                 855                 860                 


Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala 
865                 870                 875                 880 


Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala 
                885                 890                 895     


Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly 
            900                 905                 910         


Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys 
        915                 920                 925             


Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg Thr Thr Gln Lys 
    930                 935                 940                 


Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile 
945                 950                 955                 960 


Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr 
                965                 970                 975     


Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg 
            980                 985                 990         


Asp Met Tyr Val Asp Gln Glu Leu  Asp Ile Asn Arg Leu  Ser Asp Tyr 
        995                 1000                 1005             


Asp Val  Asp His Ile Val Pro  Gln Ser Phe Leu Lys  Asp Asp Ser 
    1010                 1015                 1020             


Ile Asp  Asn Lys Val Leu Thr  Arg Ser Asp Lys Asn  Arg Gly Lys 
    1025                 1030                 1035             


Ser Asp  Asn Val Pro Ser Glu  Glu Val Val Lys Lys  Met Lys Asn 
    1040                 1045                 1050             


Tyr Trp  Arg Gln Leu Leu Asn  Ala Lys Leu Ile Thr  Gln Arg Lys 
    1055                 1060                 1065             


Phe Asp  Asn Leu Thr Lys Ala  Glu Arg Gly Gly Leu  Ser Glu Leu 
    1070                 1075                 1080             


Asp Lys  Ala Gly Phe Ile Lys  Arg Gln Leu Val Glu  Thr Arg Gln 
    1085                 1090                 1095             


Ile Thr  Lys His Val Ala Gln  Ile Leu Asp Ser Arg  Met Asn Thr 
    1100                 1105                 1110             


Lys Tyr  Asp Glu Asn Asp Lys  Leu Ile Arg Glu Val  Lys Val Ile 
    1115                 1120                 1125             


Thr Leu  Lys Ser Lys Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln 
    1130                 1135                 1140             


Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr His His  Ala His Asp 
    1145                 1150                 1155             


Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr 
    1160                 1165                 1170             


Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr 
    1175                 1180                 1185             


Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys 
    1190                 1195                 1200             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1205                 1210                 1215             


Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro 
    1220                 1225                 1230             


Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys 
    1235                 1240                 1245             


Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val Leu Ser  Met Pro Gln 
    1250                 1255                 1260             


Val Asn  Ile Val Lys Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser 
    1265                 1270                 1275             


Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala 
    1280                 1285                 1290             


Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1295                 1300                 1305             


Pro Thr  Val Ala Tyr Ser Val  Leu Val Val Ala Lys  Val Glu Lys 
    1310                 1315                 1320             


Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile 
    1325                 1330                 1335             


Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe 
    1340                 1345                 1350             


Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile 
    1355                 1360                 1365             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys 
    1370                 1375                 1380             


Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu 
    1385                 1390                 1395             


Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His 
    1400                 1405                 1410             


Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln 
    1415                 1420                 1425             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1430                 1435                 1440             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1445                 1450                 1455             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1460                 1465                 1470             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1475                 1480                 1485             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1490                 1495                 1500             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1505                 1510                 1515             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1520                 1525                 1530             


Leu Ser  Gln Leu Gly Gly Asp  
    1535                 1540 


<210>  287
<211>  1540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.1 Cas9 TadAins 1250 
       polypeptide sequence

<400>  287

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Glu  Thr Pro Gly Thr Ser  Glu Ser Ala 
    1250                 1255                 1260             


Thr Pro  Glu Ser Ser Gly Ser  Glu Val Glu Phe Ser  His Glu Tyr 
    1265                 1270                 1275             


Trp Met  Arg His Ala Leu Thr  Leu Ala Lys Arg Ala  Arg Asp Glu 
    1280                 1285                 1290             


Arg Glu  Val Pro Val Gly Ala  Val Leu Val Leu Asn  Asn Arg Val 
    1295                 1300                 1305             


Ile Gly  Glu Gly Trp Asn Arg  Ala Ile Gly Leu His  Asp Pro Thr 
    1310                 1315                 1320             


Ala His  Ala Glu Ile Met Ala  Leu Arg Gln Gly Gly  Leu Val Met 
    1325                 1330                 1335             


Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu Tyr Val  Thr Phe Glu 
    1340                 1345                 1350             


Pro Cys  Val Met Cys Ala Gly  Ala Met Ile His Ser  Arg Ile Gly 
    1355                 1360                 1365             


Arg Val  Val Phe Gly Val Arg  Asn Ala Lys Thr Gly  Ala Ala Gly 
    1370                 1375                 1380             


Ser Leu  Met Asp Val Leu His  Tyr Pro Gly Met Asn  His Arg Val 
    1385                 1390                 1395             


Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu 
    1400                 1405                 1410             


Cys Tyr  Phe Phe Arg Met Pro  Arg Glu Asp Asn Glu  Gln Lys Gln 
    1415                 1420                 1425             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1430                 1435                 1440             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1445                 1450                 1455             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1460                 1465                 1470             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1475                 1480                 1485             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1490                 1495                 1500             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1505                 1510                 1515             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1520                 1525                 1530             


Leu Ser  Gln Leu Gly Gly Asp  
    1535                 1540 


<210>  288
<211>  1543
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.2 Cas9 TadAins 1250 
       polypeptide sequence

<400>  288

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser 
    1250                 1255                 1260             


Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Ser Glu Val  Glu Phe Ser 
    1265                 1270                 1275             


His Glu  Tyr Trp Met Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala 
    1280                 1285                 1290             


Arg Asp  Glu Arg Glu Val Pro  Val Gly Ala Val Leu  Val Leu Asn 
    1295                 1300                 1305             


Asn Arg  Val Ile Gly Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His 
    1310                 1315                 1320             


Asp Pro  Thr Ala His Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly 
    1325                 1330                 1335             


Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp Ala Thr  Leu Tyr Val 
    1340                 1345                 1350             


Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly Ala Met  Ile His Ser 
    1355                 1360                 1365             


Arg Ile  Gly Arg Val Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly 
    1370                 1375                 1380             


Ala Ala  Gly Ser Leu Met Asp  Val Leu His Tyr Pro  Gly Met Asn 
    1385                 1390                 1395             


His Arg  Val Glu Ile Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala 
    1400                 1405                 1410             


Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro Arg Glu  Asp Asn Glu 
    1415                 1420                 1425             


Gln Lys  Gln Leu Phe Val Glu  Gln His Lys His Tyr  Leu Asp Glu 
    1430                 1435                 1440             


Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys Arg Val  Ile Leu Ala 
    1445                 1450                 1455             


Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala Tyr Asn  Lys His Arg 
    1460                 1465                 1470             


Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn Ile Ile  His Leu Phe 
    1475                 1480                 1485             


Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala Phe Lys  Tyr Phe Asp 
    1490                 1495                 1500             


Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu 
    1505                 1510                 1515             


Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr 
    1520                 1525                 1530             


Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1535                 1540             


<210>  289
<211>  1546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.3 Cas9 TadAins 1250 
       polypeptide sequence

<400>  289

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser 
    1250                 1255                 1260             


Glu Ser  Ala Thr Pro Glu Ser  Gly Ser Ser Ser Gly  Ser Glu Val 
    1265                 1270                 1275             


Glu Phe  Ser His Glu Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala 
    1280                 1285                 1290             


Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro Val Gly  Ala Val Leu 
    1295                 1300                 1305             


Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile 
    1310                 1315                 1320             


Gly Leu  His Asp Pro Thr Ala  His Ala Glu Ile Met  Ala Leu Arg 
    1325                 1330                 1335             


Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg Leu Ile  Asp Ala Thr 
    1340                 1345                 1350             


Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met Cys Ala  Gly Ala Met 
    1355                 1360                 1365             


Ile His  Ser Arg Ile Gly Arg  Val Val Phe Gly Val  Arg Asn Ala 
    1370                 1375                 1380             


Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp Val Leu  His Tyr Pro 
    1385                 1390                 1395             


Gly Met  Asn His Arg Val Glu  Ile Thr Glu Gly Ile  Leu Ala Asp 
    1400                 1405                 1410             


Glu Cys  Ala Ala Leu Leu Cys  Tyr Phe Phe Arg Met  Pro Arg Glu 
    1415                 1420                 1425             


Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1430                 1435                 1440             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1445                 1450                 1455             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1460                 1465                 1470             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1475                 1480                 1485             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1490                 1495                 1500             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1505                 1510                 1515             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1520                 1525                 1530             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540                 1545     


<210>  290
<211>  1546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.4 Cas9 TadAins 1250 
       polypeptide sequence

<400>  290

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser 
    1250                 1255                 1260             


Glu Ser  Ala Thr Pro Glu Ser  Gly Ser Ser Ser Gly  Ser Glu Val 
    1265                 1270                 1275             


Glu Phe  Ser His Glu Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala 
    1280                 1285                 1290             


Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro Val Gly  Ala Val Leu 
    1295                 1300                 1305             


Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile 
    1310                 1315                 1320             


Gly Leu  His Asp Pro Thr Ala  His Ala Glu Ile Met  Ala Leu Arg 
    1325                 1330                 1335             


Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg Leu Ile  Asp Ala Thr 
    1340                 1345                 1350             


Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met Cys Ala  Gly Ala Met 
    1355                 1360                 1365             


Ile His  Ser Arg Ile Gly Arg  Val Val Phe Gly Val  Arg Asn Ala 
    1370                 1375                 1380             


Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp Val Leu  His Tyr Pro 
    1385                 1390                 1395             


Gly Met  Asn His Arg Val Glu  Ile Thr Glu Gly Ile  Leu Ala Asp 
    1400                 1405                 1410             


Glu Cys  Ala Ala Leu Leu Cys  Tyr Phe Phe Arg Met  Arg Arg Glu 
    1415                 1420                 1425             


Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1430                 1435                 1440             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1445                 1450                 1455             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1460                 1465                 1470             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1475                 1480                 1485             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1490                 1495                 1500             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1505                 1510                 1515             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1520                 1525                 1530             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540                 1545     


<210>  291
<211>  1546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.5 Cas9 TadAins 1249 
       polypeptide sequence

<400>  291

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Gly Ser  Ser Gly Ser Ser Gly  Ser Glu Thr Pro Gly  Thr Ser Glu 
    1250                 1255                 1260             


Ser Ala  Thr Pro Glu Ser Gly  Ser Ser Ser Gly Ser  Glu Val Glu 
    1265                 1270                 1275             


Phe Ser  His Glu Tyr Trp Met  Arg His Ala Leu Thr  Leu Ala Lys 
    1280                 1285                 1290             


Arg Ala  Arg Asp Glu Arg Glu  Val Pro Val Gly Ala  Val Leu Val 
    1295                 1300                 1305             


Leu Asn  Asn Arg Val Ile Gly  Glu Gly Trp Asn Arg  Ala Ile Gly 
    1310                 1315                 1320             


Leu His  Asp Pro Thr Ala His  Ala Glu Ile Met Ala  Leu Arg Gln 
    1325                 1330                 1335             


Gly Gly  Leu Val Met Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu 
    1340                 1345                 1350             


Tyr Val  Thr Phe Glu Pro Cys  Val Met Cys Ala Gly  Ala Met Ile 
    1355                 1360                 1365             


His Ser  Arg Ile Gly Arg Val  Val Phe Gly Val Arg  Asn Ala Lys 
    1370                 1375                 1380             


Thr Gly  Ala Ala Gly Ser Leu  Met Asp Val Leu His  Tyr Pro Gly 
    1385                 1390                 1395             


Met Asn  His Arg Val Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu 
    1400                 1405                 1410             


Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg Met Arg  Arg Pro Glu 
    1415                 1420                 1425             


Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1430                 1435                 1440             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1445                 1450                 1455             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1460                 1465                 1470             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1475                 1480                 1485             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1490                 1495                 1500             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1505                 1510                 1515             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1520                 1525                 1530             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540                 1545     


<210>  292
<211>  1551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.5 Cas9 TadAins delta 
       59-66 1250 polypeptide sequence

<400>  292

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser 
    1250                 1255                 1260             


Glu Ser  Ala Thr Pro Glu Ser  Gly Ser Ser Gly Ser  Glu Val Glu 
    1265                 1270                 1275             


Phe Ser  His Glu Tyr Trp Met  Arg His Ala Leu Thr  Leu Ala Lys 
    1280                 1285                 1290             


Arg Ala  Arg Asp Glu Arg Glu  Val Pro Val Gly Ala  Val Leu Val 
    1295                 1300                 1305             


Leu Asn  Asn Arg Val Ile Gly  Glu Gly Trp Asn Arg  Ala His Ala 
    1310                 1315                 1320             


Glu Ile  Met Ala Leu Arg Gln  Gly Gly Leu Val Met  Gln Asn Tyr 
    1325                 1330                 1335             


Arg Leu  Ile Asp Ala Thr Leu  Tyr Val Thr Phe Glu  Pro Cys Val 
    1340                 1345                 1350             


Met Cys  Ala Gly Ala Met Ile  His Ser Arg Ile Gly  Arg Val Val 
    1355                 1360                 1365             


Phe Gly  Val Arg Asn Ala Lys  Thr Gly Ala Ala Gly  Ser Leu Met 
    1370                 1375                 1380             


Asp Val  Leu His Tyr Pro Gly  Met Asn His Arg Val  Glu Ile Thr 
    1385                 1390                 1395             


Glu Gly  Ile Leu Ala Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe 
    1400                 1405                 1410             


Phe Arg  Met Pro Arg Gln Val  Phe Asn Ala Gln Lys  Lys Ala Gln 
    1415                 1420                 1425             


Ser Ser  Thr Asp Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1430                 1435                 1440             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1445                 1450                 1455             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1460                 1465                 1470             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1475                 1480                 1485             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1490                 1495                 1500             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1505                 1510                 1515             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1520                 1525                 1530             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1535                 1540                 1545             


Gly Gly  Asp 
    1550     


<210>  293
<211>  1546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.6 Cas9 TadAins 1251 
       polypeptide sequence

<400>  293

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Gly Ser Ser Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr 
    1250                 1255                 1260             


Ser Glu  Ser Ala Thr Pro Glu  Ser Gly Ser Ser Ser  Gly Ser Glu 
    1265                 1270                 1275             


Val Glu  Phe Ser His Glu Tyr  Trp Met Arg His Ala  Leu Thr Leu 
    1280                 1285                 1290             


Ala Lys  Arg Ala Arg Asp Glu  Arg Glu Val Pro Val  Gly Ala Val 
    1295                 1300                 1305             


Leu Val  Leu Asn Asn Arg Val  Ile Gly Glu Gly Trp  Asn Arg Ala 
    1310                 1315                 1320             


Ile Gly  Leu His Asp Pro Thr  Ala His Ala Glu Ile  Met Ala Leu 
    1325                 1330                 1335             


Arg Gln  Gly Gly Leu Val Met  Gln Asn Tyr Arg Leu  Ile Asp Ala 
    1340                 1345                 1350             


Thr Leu  Tyr Val Thr Phe Glu  Pro Cys Val Met Cys  Ala Gly Ala 
    1355                 1360                 1365             


Met Ile  His Ser Arg Ile Gly  Arg Val Val Phe Gly  Val Arg Asn 
    1370                 1375                 1380             


Ala Lys  Thr Gly Ala Ala Gly  Ser Leu Met Asp Val  Leu His Tyr 
    1385                 1390                 1395             


Pro Gly  Met Asn His Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala 
    1400                 1405                 1410             


Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe Phe Arg  Met Arg Arg 
    1415                 1420                 1425             


Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1430                 1435                 1440             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1445                 1450                 1455             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1460                 1465                 1470             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1475                 1480                 1485             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1490                 1495                 1500             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1505                 1510                 1515             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1520                 1525                 1530             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540                 1545     


<210>  294
<211>  1546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.7 Cas9 TadAins 1252 
       polypeptide sequence

<400>  294

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Gly Ser Ser Gly  Ser Ser Gly Ser Glu  Thr Pro Gly 
    1250                 1255                 1260             


Thr Ser  Glu Ser Ala Thr Pro  Glu Ser Gly Ser Ser  Ser Gly Ser 
    1265                 1270                 1275             


Glu Val  Glu Phe Ser His Glu  Tyr Trp Met Arg His  Ala Leu Thr 
    1280                 1285                 1290             


Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu Val Pro  Val Gly Ala 
    1295                 1300                 1305             


Val Leu  Val Leu Asn Asn Arg  Val Ile Gly Glu Gly  Trp Asn Arg 
    1310                 1315                 1320             


Ala Ile  Gly Leu His Asp Pro  Thr Ala His Ala Glu  Ile Met Ala 
    1325                 1330                 1335             


Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp 
    1340                 1345                 1350             


Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly 
    1355                 1360                 1365             


Ala Met  Ile His Ser Arg Ile  Gly Arg Val Val Phe  Gly Val Arg 
    1370                 1375                 1380             


Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu Met Asp  Val Leu His 
    1385                 1390                 1395             


Tyr Pro  Gly Met Asn His Arg  Val Glu Ile Thr Glu  Gly Ile Leu 
    1400                 1405                 1410             


Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Arg 
    1415                 1420                 1425             


Arg Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1430                 1435                 1440             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1445                 1450                 1455             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1460                 1465                 1470             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1475                 1480                 1485             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1490                 1495                 1500             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1505                 1510                 1515             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1520                 1525                 1530             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540                 1545     


<210>  295
<211>  1533
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 110.8 Cas9 TadAins delta 
       59-66 C-truncate 1250 polypeptide sequence

<400>  295

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Gly  Ser Ser Gly Ser Glu  Thr Pro Gly Thr Ser  Glu Ser Ala 
    1250                 1255                 1260             


Thr Pro  Glu Ser Ser Gly Ser  Glu Val Glu Phe Ser  His Glu Tyr 
    1265                 1270                 1275             


Trp Met  Arg His Ala Leu Thr  Leu Ala Lys Arg Ala  Arg Asp Glu 
    1280                 1285                 1290             


Arg Glu  Val Pro Val Gly Ala  Val Leu Val Leu Asn  Asn Arg Val 
    1295                 1300                 1305             


Ile Gly  Glu Gly Trp Asn Arg  Ala His Ala Glu Ile  Met Ala Leu 
    1310                 1315                 1320             


Arg Gln  Gly Gly Leu Val Met  Gln Asn Tyr Arg Leu  Ile Asp Ala 
    1325                 1330                 1335             


Thr Leu  Tyr Val Thr Phe Glu  Pro Cys Val Met Cys  Ala Gly Ala 
    1340                 1345                 1350             


Met Ile  His Ser Arg Ile Gly  Arg Val Val Phe Gly  Val Arg Asn 
    1355                 1360                 1365             


Ala Lys  Thr Gly Ala Ala Gly  Ser Leu Met Asp Val  Leu His Tyr 
    1370                 1375                 1380             


Pro Gly  Met Asn His Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala 
    1385                 1390                 1395             


Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe Phe Arg  Met Pro Arg 
    1400                 1405                 1410             


Gln Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1415                 1420                 1425             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1430                 1435                 1440             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1445                 1450                 1455             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1460                 1465                 1470             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1475                 1480                 1485             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1490                 1495                 1500             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1505                 1510                 1515             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1520                 1525                 1530             


<210>  296
<211>  1549
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 111.1 Cas9 TadAins 997 
       polypeptide sequence

<400>  296

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ser His Glu  Tyr Trp Met Arg His  Ala Leu Thr 
        995                 1000                 1005             


Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu Val Pro  Val Gly Ala 
    1010                 1015                 1020             


Val Leu  Val Leu Asn Asn Arg  Val Ile Gly Glu Gly  Trp Asn Arg 
    1025                 1030                 1035             


Ala Ile  Gly Leu His Asp Pro  Thr Ala His Ala Glu  Ile Met Ala 
    1040                 1045                 1050             


Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp 
    1055                 1060                 1065             


Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly 
    1070                 1075                 1080             


Ala Met  Ile His Ser Arg Ile  Gly Arg Val Val Phe  Gly Val Arg 
    1085                 1090                 1095             


Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu Met Asp  Val Leu His 
    1100                 1105                 1110             


Tyr Pro  Gly Met Asn His Arg  Val Glu Ile Thr Glu  Gly Ile Leu 
    1115                 1120                 1125             


Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro 
    1130                 1135                 1140             


Arg Gln  Val Phe Asn Ala Gln  Lys Lys Ala Gln Ser  Ser Thr Asp 
    1145                 1150                 1155             


Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr 
    1160                 1165                 1170             


Pro Glu  Ser Ser Gly Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1175                 1180                 1185             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1190                 1195                 1200             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1205                 1210                 1215             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1220                 1225                 1230             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1235                 1240                 1245             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1250                 1255                 1260             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1265                 1270                 1275             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1280                 1285                 1290             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1295                 1300                 1305             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1310                 1315                 1320             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1325                 1330                 1335             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1340                 1345                 1350             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1355                 1360                 1365             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1370                 1375                 1380             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1385                 1390                 1395             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1400                 1405                 1410             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1415                 1420                 1425             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1430                 1435                 1440             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1445                 1450                 1455             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1460                 1465                 1470             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1475                 1480                 1485             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1490                 1495                 1500             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1505                 1510                 1515             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1520                 1525                 1530             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1535                 1540                 1545             


Asp 
    


<210>  297
<211>  1555
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 111.2 Cas9 TadAins 997 
       polypeptide sequence

<400>  297

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ser His Glu  Tyr Trp Met Arg His  Ala Leu Thr 
        995                 1000                 1005             


Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu Val Pro  Val Gly Ala 
    1010                 1015                 1020             


Val Leu  Val Leu Asn Asn Arg  Val Ile Gly Glu Gly  Trp Asn Arg 
    1025                 1030                 1035             


Ala Ile  Gly Leu His Asp Pro  Thr Ala His Ala Glu  Ile Met Ala 
    1040                 1045                 1050             


Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp 
    1055                 1060                 1065             


Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly 
    1070                 1075                 1080             


Ala Met  Ile His Ser Arg Ile  Gly Arg Val Val Phe  Gly Val Arg 
    1085                 1090                 1095             


Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu Met Asp  Val Leu His 
    1100                 1105                 1110             


Tyr Pro  Gly Met Asn His Arg  Val Glu Ile Thr Glu  Gly Ile Leu 
    1115                 1120                 1125             


Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro 
    1130                 1135                 1140             


Arg Gln  Val Phe Asn Ala Gln  Lys Lys Ala Gln Ser  Ser Thr Asp 
    1145                 1150                 1155             


Gly Ser  Ser Gly Ser Ser Gly  Ser Glu Thr Pro Gly  Thr Ser Glu 
    1160                 1165                 1170             


Ser Ala  Thr Pro Glu Ser Ser  Gly Gly Ser Ser Ile  Lys Lys Tyr 
    1175                 1180                 1185             


Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr 
    1190                 1195                 1200             


Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys 
    1205                 1210                 1215             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1220                 1225                 1230             


Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro 
    1235                 1240                 1245             


Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys 
    1250                 1255                 1260             


Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val Leu Ser  Met Pro Gln 
    1265                 1270                 1275             


Val Asn  Ile Val Lys Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser 
    1280                 1285                 1290             


Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala 
    1295                 1300                 1305             


Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1310                 1315                 1320             


Pro Thr  Val Ala Tyr Ser Val  Leu Val Val Ala Lys  Val Glu Lys 
    1325                 1330                 1335             


Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile 
    1340                 1345                 1350             


Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe 
    1355                 1360                 1365             


Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile 
    1370                 1375                 1380             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys 
    1385                 1390                 1395             


Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu 
    1400                 1405                 1410             


Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His 
    1415                 1420                 1425             


Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln 
    1430                 1435                 1440             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1445                 1450                 1455             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1460                 1465                 1470             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1475                 1480                 1485             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1490                 1495                 1500             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1505                 1510                 1515             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1520                 1525                 1530             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1535                 1540                 1545             


Leu Ser  Gln Leu Gly Gly Asp  
    1550                 1555 


<210>  298
<211>  1421
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 112 delta HNH TadA 
       polypeptide sequence

<400>  298

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Glu Val Glu Phe Ser His Glu 
785                 790                 795                 800 


Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu 
                805                 810                 815     


Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile 
            820                 825                 830         


Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His 
        835                 840                 845             


Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr 
    850                 855                 860                 


Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met 
865                 870                 875                 880 


Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly 
                885                 890                 895     


Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu 
            900                 905                 910         


His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu 
        915                 920                 925             


Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg 
    930                 935                 940                 


Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Gly Gly 
945                 950                 955                 960 


Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
                965                 970                 975     


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met 
            980                 985                 990         


Asn Thr Lys Tyr Asp Glu Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val 
        995                 1000                 1005             


Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe 
    1010                 1015                 1020             


Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn Tyr His  His Ala His 
    1025                 1030                 1035             


Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys 
    1040                 1045                 1050             


Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1055                 1060                 1065             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1070                 1075                 1080             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1085                 1090                 1095             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1100                 1105                 1110             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1115                 1120                 1125             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1130                 1135                 1140             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1145                 1150                 1155             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1160                 1165                 1170             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1175                 1180                 1185             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1190                 1195                 1200             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1205                 1210                 1215             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1220                 1225                 1230             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1235                 1240                 1245             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1250                 1255                 1260             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1265                 1270                 1275             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1280                 1285                 1290             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1295                 1300                 1305             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1310                 1315                 1320             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1325                 1330                 1335             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1340                 1345                 1350             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1355                 1360                 1365             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1370                 1375                 1380             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1385                 1390                 1395             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1400                 1405                 1410             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1415                 1420     


<210>  299
<211>  1552
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 113 N-term single TadA helix 
       trunc 165-end polypeptide sequence

<400>  299

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Ser Gly Gly Ser Ser Gly Gly 
145                 150                 155                 160 


Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
                165                 170                 175     


Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly 
            180                 185                 190         


Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
        195                 200                 205             


Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
    210                 215                 220                 


His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
225                 230                 235                 240 


Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
                245                 250                 255     


Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
            260                 265                 270         


Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
        275                 280                 285             


Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
    290                 295                 300                 


Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
305                 310                 315                 320 


His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
                325                 330                 335     


Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
            340                 345                 350         


Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
        355                 360                 365             


Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
    370                 375                 380                 


Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
385                 390                 395                 400 


Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
                405                 410                 415     


Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
            420                 425                 430         


Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
        435                 440                 445             


Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
    450                 455                 460                 


Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
465                 470                 475                 480 


Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
                485                 490                 495     


Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
            500                 505                 510         


His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
        515                 520                 525             


Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
    530                 535                 540                 


Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
545                 550                 555                 560 


Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
                565                 570                 575     


Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
            580                 585                 590         


Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
        595                 600                 605             


Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
    610                 615                 620                 


Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
625                 630                 635                 640 


Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
                645                 650                 655     


Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
            660                 665                 670         


Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn 
        675                 680                 685             


Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
    690                 695                 700                 


Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
705                 710                 715                 720 


Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
                725                 730                 735     


Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
            740                 745                 750         


Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
        755                 760                 765             


Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
    770                 775                 780                 


Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
785                 790                 795                 800 


Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile 
                805                 810                 815     


Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met 
            820                 825                 830         


Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg 
        835                 840                 845             


Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu 
    850                 855                 860                 


Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu 
865                 870                 875                 880 


Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln 
                885                 890                 895     


Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala 
            900                 905                 910         


Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val 
        915                 920                 925             


Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val 
    930                 935                 940                 


Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn 
945                 950                 955                 960 


Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly 
                965                 970                 975     


Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn 
            980                 985                 990         


Glu Lys Leu Tyr Leu Tyr Tyr Leu  Gln Asn Gly Arg Asp  Met Tyr Val 
        995                 1000                 1005             


Asp Gln  Glu Leu Asp Ile Asn  Arg Leu Ser Asp Tyr  Asp Val Asp 
    1010                 1015                 1020             


His Ile  Val Pro Gln Ser Phe  Leu Lys Asp Asp Ser  Ile Asp Asn 
    1025                 1030                 1035             


Lys Val  Leu Thr Arg Ser Asp  Lys Asn Arg Gly Lys  Ser Asp Asn 
    1040                 1045                 1050             


Val Pro  Ser Glu Glu Val Val  Lys Lys Met Lys Asn  Tyr Trp Arg 
    1055                 1060                 1065             


Gln Leu  Leu Asn Ala Lys Leu  Ile Thr Gln Arg Lys  Phe Asp Asn 
    1070                 1075                 1080             


Leu Thr  Lys Ala Glu Arg Gly  Gly Leu Ser Glu Leu  Asp Lys Ala 
    1085                 1090                 1095             


Gly Phe  Ile Lys Arg Gln Leu  Val Glu Thr Arg Gln  Ile Thr Lys 
    1100                 1105                 1110             


His Val  Ala Gln Ile Leu Asp  Ser Arg Met Asn Thr  Lys Tyr Asp 
    1115                 1120                 1125             


Glu Asn  Asp Lys Leu Ile Arg  Glu Val Lys Val Ile  Thr Leu Lys 
    1130                 1135                 1140             


Ser Lys  Leu Val Ser Asp Phe  Arg Lys Asp Phe Gln  Phe Tyr Lys 
    1145                 1150                 1155             


Val Arg  Glu Ile Asn Asn Tyr  His His Ala His Asp  Ala Tyr Leu 
    1160                 1165                 1170             


Asn Ala  Val Val Gly Thr Ala  Leu Ile Lys Lys Tyr  Pro Lys Leu 
    1175                 1180                 1185             


Glu Ser  Glu Phe Val Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg 
    1190                 1195                 1200             


Lys Met  Ile Ala Lys Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala 
    1205                 1210                 1215             


Lys Tyr  Phe Phe Tyr Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu 
    1220                 1225                 1230             


Ile Thr  Leu Ala Asn Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu 
    1235                 1240                 1245             


Thr Asn  Gly Glu Thr Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp 
    1250                 1255                 1260             


Phe Ala  Thr Val Arg Lys Val  Leu Ser Met Pro Gln  Val Asn Ile 
    1265                 1270                 1275             


Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser 
    1280                 1285                 1290             


Ile Leu  Pro Lys Arg Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys 
    1295                 1300                 1305             


Asp Trp  Asp Pro Lys Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val 
    1310                 1315                 1320             


Ala Tyr  Ser Val Leu Val Val  Ala Lys Val Glu Lys  Gly Lys Ser 
    1325                 1330                 1335             


Lys Lys  Leu Lys Ser Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met 
    1340                 1345                 1350             


Glu Arg  Ser Ser Phe Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala 
    1355                 1360                 1365             


Lys Gly  Tyr Lys Glu Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro 
    1370                 1375                 1380             


Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu 
    1385                 1390                 1395             


Ala Ser  Ala Gly Glu Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro 
    1400                 1405                 1410             


Ser Lys  Tyr Val Asn Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys 
    1415                 1420                 1425             


Leu Lys  Gly Ser Pro Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val 
    1430                 1435                 1440             


Glu Gln  His Lys His Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser 
    1445                 1450                 1455             


Glu Phe  Ser Lys Arg Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys 
    1460                 1465                 1470             


Val Leu  Ser Ala Tyr Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu 
    1475                 1480                 1485             


Gln Ala  Glu Asn Ile Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly 
    1490                 1495                 1500             


Ala Pro  Ala Ala Phe Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys 
    1505                 1510                 1515             


Arg Tyr  Thr Ser Thr Lys Glu  Val Leu Asp Ala Thr  Leu Ile His 
    1520                 1525                 1530             


Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln 
    1535                 1540                 1545             


Leu Gly  Gly Asp 
    1550         


<210>  300
<211>  1545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 114 N-term single TadA helix 
       trunc 165-end delta 59-65 polypeptide sequence

<400>  300

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Thr 
        35                  40                  45              


Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln 
    50                  55                  60                  


Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys 
65                  70                  75                  80  


Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val 
                85                  90                  95      


Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp 
            100                 105                 110         


Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly 
        115                 120                 125             


Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met 
    130                 135                 140                 


Pro Arg Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro 
145                 150                 155                 160 


Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly 
                165                 170                 175     


Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser 
            180                 185                 190         


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        195                 200                 205             


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    210                 215                 220                 


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
225                 230                 235                 240 


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                245                 250                 255     


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            260                 265                 270         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        275                 280                 285             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    290                 295                 300                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
305                 310                 315                 320 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                325                 330                 335     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            340                 345                 350         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        355                 360                 365             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    370                 375                 380                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
385                 390                 395                 400 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                405                 410                 415     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            420                 425                 430         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        435                 440                 445             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    450                 455                 460                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
465                 470                 475                 480 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                485                 490                 495     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            500                 505                 510         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        515                 520                 525             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    530                 535                 540                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
545                 550                 555                 560 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                565                 570                 575     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            580                 585                 590         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        595                 600                 605             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    610                 615                 620                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
625                 630                 635                 640 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                645                 650                 655     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            660                 665                 670         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        675                 680                 685             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    690                 695                 700                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
705                 710                 715                 720 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                725                 730                 735     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            740                 745                 750         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        755                 760                 765             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    770                 775                 780                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
785                 790                 795                 800 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                805                 810                 815     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            820                 825                 830         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        835                 840                 845             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    850                 855                 860                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
865                 870                 875                 880 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                885                 890                 895     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            900                 905                 910         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        915                 920                 925             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    930                 935                 940                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
945                 950                 955                 960 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                965                 970                 975     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            980                 985                 990         


Leu Gln Asn Gly Arg Asp Met Tyr  Val Asp Gln Glu Leu  Asp Ile Asn 
        995                 1000                 1005             


Arg Leu  Ser Asp Tyr Asp Val  Asp His Ile Val Pro  Gln Ser Phe 
    1010                 1015                 1020             


Leu Lys  Asp Asp Ser Ile Asp  Asn Lys Val Leu Thr  Arg Ser Asp 
    1025                 1030                 1035             


Lys Asn  Arg Gly Lys Ser Asp  Asn Val Pro Ser Glu  Glu Val Val 
    1040                 1045                 1050             


Lys Lys  Met Lys Asn Tyr Trp  Arg Gln Leu Leu Asn  Ala Lys Leu 
    1055                 1060                 1065             


Ile Thr  Gln Arg Lys Phe Asp  Asn Leu Thr Lys Ala  Glu Arg Gly 
    1070                 1075                 1080             


Gly Leu  Ser Glu Leu Asp Lys  Ala Gly Phe Ile Lys  Arg Gln Leu 
    1085                 1090                 1095             


Val Glu  Thr Arg Gln Ile Thr  Lys His Val Ala Gln  Ile Leu Asp 
    1100                 1105                 1110             


Ser Arg  Met Asn Thr Lys Tyr  Asp Glu Asn Asp Lys  Leu Ile Arg 
    1115                 1120                 1125             


Glu Val  Lys Val Ile Thr Leu  Lys Ser Lys Leu Val  Ser Asp Phe 
    1130                 1135                 1140             


Arg Lys  Asp Phe Gln Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr 
    1145                 1150                 1155             


His His  Ala His Asp Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala 
    1160                 1165                 1170             


Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly 
    1175                 1180                 1185             


Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu 
    1190                 1195                 1200             


Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn 
    1205                 1210                 1215             


Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu 
    1220                 1225                 1230             


Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu 
    1235                 1240                 1245             


Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val 
    1250                 1255                 1260             


Leu Ser  Met Pro Gln Val Asn  Ile Val Lys Lys Thr  Glu Val Gln 
    1265                 1270                 1275             


Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser 
    1280                 1285                 1290             


Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr 
    1295                 1300                 1305             


Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr Ser Val  Leu Val Val 
    1310                 1315                 1320             


Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys 
    1325                 1330                 1335             


Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys 
    1340                 1345                 1350             


Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys 
    1355                 1360                 1365             


Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu 
    1370                 1375                 1380             


Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln 
    1385                 1390                 1395             


Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu 
    1400                 1405                 1410             


Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp 
    1415                 1420                 1425             


Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln His Lys  His Tyr Leu 
    1430                 1435                 1440             


Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile 
    1445                 1450                 1455             


Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys 
    1460                 1465                 1470             


His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His 
    1475                 1480                 1485             


Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr 
    1490                 1495                 1500             


Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu 
    1505                 1510                 1515             


Val Leu  Asp Ala Thr Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr 
    1520                 1525                 1530             


Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly Gly Asp  
    1535                 1540                 1545 


<210>  301
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 115.1 Cas9 TadAins1004 
       polypeptide sequence

<400>  301

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Gly Ser  Ser Gly Ser 
        995                 1000                 1005             


Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr Pro Glu  Ser Ser Gly 
    1010                 1015                 1020             


Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp Met Arg  His Ala Leu 
    1025                 1030                 1035             


Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg Glu Val  Pro Val Gly 
    1040                 1045                 1050             


Ala Val  Leu Val Leu Asn Asn  Arg Val Ile Gly Glu  Gly Trp Asn 
    1055                 1060                 1065             


Arg Ala  Ile Gly Leu His Asp  Pro Thr Ala His Ala  Glu Ile Met 
    1070                 1075                 1080             


Ala Leu  Arg Gln Gly Gly Leu  Val Met Gln Asn Tyr  Arg Leu Ile 
    1085                 1090                 1095             


Asp Ala  Thr Leu Tyr Val Thr  Phe Glu Pro Cys Val  Met Cys Ala 
    1100                 1105                 1110             


Gly Ala  Met Ile His Ser Arg  Ile Gly Arg Val Val  Phe Gly Val 
    1115                 1120                 1125             


Arg Asn  Ala Lys Thr Gly Ala  Ala Gly Ser Leu Met  Asp Val Leu 
    1130                 1135                 1140             


His Tyr  Pro Gly Met Asn His  Arg Val Glu Ile Thr  Glu Gly Ile 
    1145                 1150                 1155             


Leu Ala  Asp Glu Cys Ala Ala  Leu Leu Cys Tyr Phe  Phe Arg Met 
    1160                 1165                 1170             


Pro Arg  Gln Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  302
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 115.2 Cas9 TadAins1005 
       polypeptide sequence

<400>  302

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Gly  Ser Ser Gly 
        995                 1000                 1005             


Ser Glu  Thr Pro Gly Thr Ser  Glu Ser Ala Thr Pro  Glu Ser Ser 
    1010                 1015                 1020             


Gly Ser  Glu Val Glu Phe Ser  His Glu Tyr Trp Met  Arg His Ala 
    1025                 1030                 1035             


Leu Thr  Leu Ala Lys Arg Ala  Arg Asp Glu Arg Glu  Val Pro Val 
    1040                 1045                 1050             


Gly Ala  Val Leu Val Leu Asn  Asn Arg Val Ile Gly  Glu Gly Trp 
    1055                 1060                 1065             


Asn Arg  Ala Ile Gly Leu His  Asp Pro Thr Ala His  Ala Glu Ile 
    1070                 1075                 1080             


Met Ala  Leu Arg Gln Gly Gly  Leu Val Met Gln Asn  Tyr Arg Leu 
    1085                 1090                 1095             


Ile Asp  Ala Thr Leu Tyr Val  Thr Phe Glu Pro Cys  Val Met Cys 
    1100                 1105                 1110             


Ala Gly  Ala Met Ile His Ser  Arg Ile Gly Arg Val  Val Phe Gly 
    1115                 1120                 1125             


Val Arg  Asn Ala Lys Thr Gly  Ala Ala Gly Ser Leu  Met Asp Val 
    1130                 1135                 1140             


Leu His  Tyr Pro Gly Met Asn  His Arg Val Glu Ile  Thr Glu Gly 
    1145                 1150                 1155             


Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg 
    1160                 1165                 1170             


Met Pro  Arg Gln Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  303
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 115.3 Cas9 TadAins1006 
       polypeptide sequence

<400>  303

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Gly Ser Ser 
        995                 1000                 1005             


Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser 
    1010                 1015                 1020             


Ser Gly  Ser Glu Val Glu Phe  Ser His Glu Tyr Trp  Met Arg His 
    1025                 1030                 1035             


Ala Leu  Thr Leu Ala Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro 
    1040                 1045                 1050             


Val Gly  Ala Val Leu Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly 
    1055                 1060                 1065             


Trp Asn  Arg Ala Ile Gly Leu  His Asp Pro Thr Ala  His Ala Glu 
    1070                 1075                 1080             


Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg 
    1085                 1090                 1095             


Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met 
    1100                 1105                 1110             


Cys Ala  Gly Ala Met Ile His  Ser Arg Ile Gly Arg  Val Val Phe 
    1115                 1120                 1125             


Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp 
    1130                 1135                 1140             


Val Leu  His Tyr Pro Gly Met  Asn His Arg Val Glu  Ile Thr Glu 
    1145                 1150                 1155             


Gly Ile  Leu Ala Asp Glu Cys  Ala Ala Leu Leu Cys  Tyr Phe Phe 
    1160                 1165                 1170             


Arg Met  Pro Arg Gln Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  304
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 115.4 Cas9 TadAins1007 
       polypeptide sequence

<400>  304

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Gly Ser 
        995                 1000                 1005             


Ser Gly  Ser Glu Thr Pro Gly  Thr Ser Glu Ser Ala  Thr Pro Glu 
    1010                 1015                 1020             


Ser Ser  Gly Ser Glu Val Glu  Phe Ser His Glu Tyr  Trp Met Arg 
    1025                 1030                 1035             


His Ala  Leu Thr Leu Ala Lys  Arg Ala Arg Asp Glu  Arg Glu Val 
    1040                 1045                 1050             


Pro Val  Gly Ala Val Leu Val  Leu Asn Asn Arg Val  Ile Gly Glu 
    1055                 1060                 1065             


Gly Trp  Asn Arg Ala Ile Gly  Leu His Asp Pro Thr  Ala His Ala 
    1070                 1075                 1080             


Glu Ile  Met Ala Leu Arg Gln  Gly Gly Leu Val Met  Gln Asn Tyr 
    1085                 1090                 1095             


Arg Leu  Ile Asp Ala Thr Leu  Tyr Val Thr Phe Glu  Pro Cys Val 
    1100                 1105                 1110             


Met Cys  Ala Gly Ala Met Ile  His Ser Arg Ile Gly  Arg Val Val 
    1115                 1120                 1125             


Phe Gly  Val Arg Asn Ala Lys  Thr Gly Ala Ala Gly  Ser Leu Met 
    1130                 1135                 1140             


Asp Val  Leu His Tyr Pro Gly  Met Asn His Arg Val  Glu Ile Thr 
    1145                 1150                 1155             


Glu Gly  Ile Leu Ala Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe 
    1160                 1165                 1170             


Phe Arg  Met Pro Arg Gln Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  305
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 116.1 Cas9 TadAins C-term 
       truncate2 792 polypeptide sequence

<400>  305

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Gly Ser Ser Gly Ser Glu Thr Pro 
785                 790                 795                 800 


Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Ser Glu Val Glu 
                805                 810                 815     


Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg 
            820                 825                 830         


Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn 
        835                 840                 845             


Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp 
    850                 855                 860                 


Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val 
865                 870                 875                 880 


Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu 
                885                 890                 895     


Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg 
            900                 905                 910         


Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu 
        915                 920                 925             


Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr 
    930                 935                 940                 


Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe 
945                 950                 955                 960 


Arg Met Pro Arg Gln Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn 
                965                 970                 975     


Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly 
            980                 985                 990         


Arg Asp Met Tyr Val Asp Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp 
        995                 1000                 1005             


Tyr Asp  Val Asp His Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp 
    1010                 1015                 1020             


Ser Ile  Asp Asn Lys Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly 
    1025                 1030                 1035             


Lys Ser  Asp Asn Val Pro Ser  Glu Glu Val Val Lys  Lys Met Lys 
    1040                 1045                 1050             


Asn Tyr  Trp Arg Gln Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg 
    1055                 1060                 1065             


Lys Phe  Asp Asn Leu Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu 
    1070                 1075                 1080             


Leu Asp  Lys Ala Gly Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg 
    1085                 1090                 1095             


Gln Ile  Thr Lys His Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn 
    1100                 1105                 1110             


Thr Lys  Tyr Asp Glu Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val 
    1115                 1120                 1125             


Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe 
    1130                 1135                 1140             


Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn Tyr His  His Ala His 
    1145                 1150                 1155             


Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys 
    1160                 1165                 1170             


Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  306
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 116.2 Cas9 TadAins C-term 
       truncate2 791 polypeptide sequence

<400>  306

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Ser Gly Ser Glu Thr Pro Gly 
785                 790                 795                 800 


Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Ser Glu Val Glu Phe 
                805                 810                 815     


Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala 
            820                 825                 830         


Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn 
        835                 840                 845             


Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro 
    850                 855                 860                 


Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met 
865                 870                 875                 880 


Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro 
                885                 890                 895     


Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val 
            900                 905                 910         


Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met 
        915                 920                 925             


Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu 
    930                 935                 940                 


Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg 
945                 950                 955                 960 


Met Pro Arg Gln Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn 
                965                 970                 975     


Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly 
            980                 985                 990         


Arg Asp Met Tyr Val Asp Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp 
        995                 1000                 1005             


Tyr Asp  Val Asp His Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp 
    1010                 1015                 1020             


Ser Ile  Asp Asn Lys Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly 
    1025                 1030                 1035             


Lys Ser  Asp Asn Val Pro Ser  Glu Glu Val Val Lys  Lys Met Lys 
    1040                 1045                 1050             


Asn Tyr  Trp Arg Gln Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg 
    1055                 1060                 1065             


Lys Phe  Asp Asn Leu Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu 
    1070                 1075                 1080             


Leu Asp  Lys Ala Gly Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg 
    1085                 1090                 1095             


Gln Ile  Thr Lys His Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn 
    1100                 1105                 1110             


Thr Lys  Tyr Asp Glu Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val 
    1115                 1120                 1125             


Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe 
    1130                 1135                 1140             


Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn Tyr His  His Ala His 
    1145                 1150                 1155             


Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys 
    1160                 1165                 1170             


Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  307
<211>  1541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 116.3 Cas9 TadAins C-term 
       truncate2 790 polypeptide sequence

<400>  307

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
785                 790                 795                 800 


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Ser Glu Val Glu Phe Ser 
                805                 810                 815     


His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg 
            820                 825                 830         


Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg 
        835                 840                 845             


Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr 
    850                 855                 860                 


Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln 
865                 870                 875                 880 


Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys 
                885                 890                 895     


Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val 
            900                 905                 910         


Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp 
        915                 920                 925             


Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly 
    930                 935                 940                 


Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met 
945                 950                 955                 960 


Pro Arg Gln Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn 
                965                 970                 975     


Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly 
            980                 985                 990         


Arg Asp Met Tyr Val Asp Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp 
        995                 1000                 1005             


Tyr Asp  Val Asp His Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp 
    1010                 1015                 1020             


Ser Ile  Asp Asn Lys Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly 
    1025                 1030                 1035             


Lys Ser  Asp Asn Val Pro Ser  Glu Glu Val Val Lys  Lys Met Lys 
    1040                 1045                 1050             


Asn Tyr  Trp Arg Gln Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg 
    1055                 1060                 1065             


Lys Phe  Asp Asn Leu Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu 
    1070                 1075                 1080             


Leu Asp  Lys Ala Gly Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg 
    1085                 1090                 1095             


Gln Ile  Thr Lys His Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn 
    1100                 1105                 1110             


Thr Lys  Tyr Asp Glu Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val 
    1115                 1120                 1125             


Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe 
    1130                 1135                 1140             


Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn Tyr His  His Ala His 
    1145                 1150                 1155             


Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys 
    1160                 1165                 1170             


Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1175                 1180                 1185             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly 
    1190                 1195                 1200             


Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe 
    1205                 1210                 1215             


Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg 
    1220                 1225                 1230             


Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp 
    1235                 1240                 1245             


Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys Val Leu  Ser Met Pro 
    1250                 1255                 1260             


Gln Val  Asn Ile Val Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe 
    1265                 1270                 1275             


Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile 
    1280                 1285                 1290             


Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp 
    1295                 1300                 1305             


Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  Lys Val Glu 
    1310                 1315                 1320             


Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly 
    1325                 1330                 1335             


Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp 
    1340                 1345                 1350             


Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
    1355                 1360                 1365             


Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg 
    1370                 1375                 1380             


Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu 
    1385                 1390                 1395             


Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser 
    1400                 1405                 1410             


His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys 
    1415                 1420                 1425             


Gln Leu  Phe Val Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile 
    1430                 1435                 1440             


Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala 
    1445                 1450                 1455             


Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys 
    1460                 1465                 1470             


Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu 
    1475                 1480                 1485             


Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr 
    1490                 1495                 1500             


Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala 
    1505                 1510                 1515             


Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile 
    1520                 1525                 1530             


Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1535                 1540     


<210>  308
<211>  1484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 117 Cas9 delta 1017-1069 
       polypeptide sequence

<400>  308

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Ser Ser Gly Ser  Glu Val Glu 
    1010                 1015                 1020             


Phe Ser  His Glu Tyr Trp Met  Arg His Ala Leu Thr  Leu Ala Lys 
    1025                 1030                 1035             


Arg Ala  Arg Asp Glu Arg Glu  Val Pro Val Gly Ala  Val Leu Val 
    1040                 1045                 1050             


Leu Asn  Asn Arg Val Ile Gly  Glu Gly Trp Asn Arg  Ala Ile Gly 
    1055                 1060                 1065             


Leu His  Asp Pro Thr Ala His  Ala Glu Ile Met Ala  Leu Arg Gln 
    1070                 1075                 1080             


Gly Gly  Leu Val Met Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu 
    1085                 1090                 1095             


Tyr Val  Thr Phe Glu Pro Cys  Val Met Cys Ala Gly  Ala Met Ile 
    1100                 1105                 1110             


His Ser  Arg Ile Gly Arg Val  Val Phe Gly Val Arg  Asn Ala Lys 
    1115                 1120                 1125             


Thr Gly  Ala Ala Gly Ser Leu  Met Asp Val Leu His  Tyr Pro Gly 
    1130                 1135                 1140             


Met Asn  His Arg Val Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu 
    1145                 1150                 1155             


Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val 
    1160                 1165                 1170             


Phe Asn  Ala Gln Lys Lys Ala  Gln Ser Ser Thr Asp  Gly Glu Ile 
    1175                 1180                 1185             


Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu 
    1190                 1195                 1200             


Ser Met  Pro Gln Val Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr 
    1205                 1210                 1215             


Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp 
    1220                 1225                 1230             


Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly 
    1235                 1240                 1245             


Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser Val Leu  Val Val Ala 
    1250                 1255                 1260             


Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu 
    1265                 1270                 1275             


Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn 
    1280                 1285                 1290             


Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
    1295                 1300                 1305             


Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu 
    1310                 1315                 1320             


Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys 
    1325                 1330                 1335             


Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr 
    1340                 1345                 1350             


Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn 
    1355                 1360                 1365             


Glu Gln  Lys Gln Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp 
    1370                 1375                 1380             


Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu 
    1385                 1390                 1395             


Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His 
    1400                 1405                 1410             


Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu 
    1415                 1420                 1425             


Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe 
    1430                 1435                 1440             


Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val 
    1445                 1450                 1455             


Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1460                 1465                 1470             


Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1475                 1480                 


<210>  309
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 118 Cas9 TadA-CP116ins 1067 
       polypeptide sequence

<400>  309

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Met Asn 
    1055                 1060                 1065             


His Arg  Val Glu Ile Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala 
    1070                 1075                 1080             


Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro Arg Gln  Val Phe Asn 
    1085                 1090                 1095             


Ala Gln  Lys Lys Ala Gln Ser  Ser Thr Asp Gly Ser  Ser Gly Ser 
    1100                 1105                 1110             


Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr Pro Glu  Ser Ser Gly 
    1115                 1120                 1125             


Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp Met Arg  His Ala Leu 
    1130                 1135                 1140             


Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg Glu Val  Pro Val Gly 
    1145                 1150                 1155             


Ala Val  Leu Val Leu Asn Asn  Arg Val Ile Gly Glu  Gly Trp Asn 
    1160                 1165                 1170             


Arg Ala  Ile Gly Leu His Asp  Pro Thr Ala His Ala  Glu Ile Met 
    1175                 1180                 1185             


Ala Leu  Arg Gln Gly Gly Leu  Val Met Gln Asn Tyr  Arg Leu Ile 
    1190                 1195                 1200             


Asp Ala  Thr Leu Tyr Val Thr  Phe Glu Pro Cys Val  Met Cys Ala 
    1205                 1210                 1215             


Gly Ala  Met Ile His Ser Arg  Ile Gly Arg Val Val  Phe Gly Val 
    1220                 1225                 1230             


Arg Asn  Ala Lys Thr Gly Ala  Ala Gly Ser Leu Met  Asp Val Leu 
    1235                 1240                 1245             


His Tyr  Pro Gly Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  310
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 119 Cas9 TadAins 701 
       polypeptide sequence

<400>  310

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Gly Ser Ser 
    690                 695                 700                 


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
705                 710                 715                 720 


Gly Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
                725                 730                 735     


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            740                 745                 750         


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        755                 760                 765             


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    770                 775                 780                 


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
785                 790                 795                 800 


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                805                 810                 815     


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            820                 825                 830         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        835                 840                 845             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    850                 855                 860                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
865                 870                 875                 880 


Lys Ala Gln Ser Ser Thr Asp Leu Thr Phe Lys Glu Asp Ile Gln Lys 
                885                 890                 895     


Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn 
            900                 905                 910         


Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys 
        915                 920                 925             


Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn 
    930                 935                 940                 


Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln 
945                 950                 955                 960 


Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu 
                965                 970                 975     


Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu 
            980                 985                 990         


Gln Asn Glu Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met 
        995                 1000                 1005             


Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp 
    1010                 1015                 1020             


Val Asp  His Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile 
    1025                 1030                 1035             


Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser 
    1040                 1045                 1050             


Asp Asn  Val Pro Ser Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr 
    1055                 1060                 1065             


Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe 
    1070                 1075                 1080             


Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp 
    1085                 1090                 1095             


Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile 
    1100                 1105                 1110             


Thr Lys  His Val Ala Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys 
    1115                 1120                 1125             


Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr 
    1130                 1135                 1140             


Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe 
    1145                 1150                 1155             


Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  His Asp Ala 
    1160                 1165                 1170             


Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro 
    1175                 1180                 1185             


Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1190                 1195                 1200             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  311
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 120 Cas9 TadACP136ins 1248 
       polypeptide sequence

<400>  311

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Met Asn  His Arg Val Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu 
    1250                 1255                 1260             


Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val 
    1265                 1270                 1275             


Phe Asn  Ala Gln Lys Lys Ala  Gln Ser Ser Thr Asp  Gly Ser Ser 
    1280                 1285                 1290             


Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser 
    1295                 1300                 1305             


Ser Gly  Ser Glu Val Glu Phe  Ser His Glu Tyr Trp  Met Arg His 
    1310                 1315                 1320             


Ala Leu  Thr Leu Ala Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro 
    1325                 1330                 1335             


Val Gly  Ala Val Leu Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly 
    1340                 1345                 1350             


Trp Asn  Arg Ala Ile Gly Leu  His Asp Pro Thr Ala  His Ala Glu 
    1355                 1360                 1365             


Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg 
    1370                 1375                 1380             


Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met 
    1385                 1390                 1395             


Cys Ala  Gly Ala Met Ile His  Ser Arg Ile Gly Arg  Val Val Phe 
    1400                 1405                 1410             


Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp 
    1415                 1420                 1425             


Val Leu  His Tyr Pro Gly Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  312
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 121 Cas9 TadACP136ins 1052 
       polypeptide sequence

<400>  312

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Met Asn  His Arg Val Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu 
    1055                 1060                 1065             


Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val 
    1070                 1075                 1080             


Phe Asn  Ala Gln Lys Lys Ala  Gln Ser Ser Thr Asp  Gly Ser Ser 
    1085                 1090                 1095             


Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser 
    1100                 1105                 1110             


Ser Gly  Ser Glu Val Glu Phe  Ser His Glu Tyr Trp  Met Arg His 
    1115                 1120                 1125             


Ala Leu  Thr Leu Ala Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro 
    1130                 1135                 1140             


Val Gly  Ala Val Leu Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly 
    1145                 1150                 1155             


Trp Asn  Arg Ala Ile Gly Leu  His Asp Pro Thr Ala  His Ala Glu 
    1160                 1165                 1170             


Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg 
    1175                 1180                 1185             


Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met 
    1190                 1195                 1200             


Cys Ala  Gly Ala Met Ile His  Ser Arg Ile Gly Arg  Val Val Phe 
    1205                 1210                 1215             


Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp 
    1220                 1225                 1230             


Val Leu  His Tyr Pro Gly Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  313
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 122 Cas9 TadACP136ins 1041 
       polypeptide sequence

<400>  313

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Met Asn His Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala 
    1040                 1045                 1050             


Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe Phe Arg  Met Pro Arg 
    1055                 1060                 1065             


Gln Val  Phe Asn Ala Gln Lys  Lys Ala Gln Ser Ser  Thr Asp Gly 
    1070                 1075                 1080             


Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro 
    1085                 1090                 1095             


Glu Ser  Ser Gly Ser Glu Val  Glu Phe Ser His Glu  Tyr Trp Met 
    1100                 1105                 1110             


Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu 
    1115                 1120                 1125             


Val Pro  Val Gly Ala Val Leu  Val Leu Asn Asn Arg  Val Ile Gly 
    1130                 1135                 1140             


Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His Asp Pro  Thr Ala His 
    1145                 1150                 1155             


Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn 
    1160                 1165                 1170             


Tyr Arg  Leu Ile Asp Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys 
    1175                 1180                 1185             


Val Met  Cys Ala Gly Ala Met  Ile His Ser Arg Ile  Gly Arg Val 
    1190                 1195                 1200             


Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu 
    1205                 1210                 1215             


Met Asp  Val Leu His Tyr Pro  Gly Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  314
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 123 Cas9 TadACP139ins 1299 
       polypeptide sequence

<400>  314

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Met Asn  His Arg Val Glu Ile  Thr Glu Gly 
    1295                 1300                 1305             


Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu Cys Tyr  Phe Phe Arg 
    1310                 1315                 1320             


Met Pro  Arg Gln Val Phe Asn  Ala Gln Lys Lys Ala  Gln Ser Ser 
    1325                 1330                 1335             


Thr Asp  Gly Ser Ser Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser 
    1340                 1345                 1350             


Ala Thr  Pro Glu Ser Ser Gly  Ser Glu Val Glu Phe  Ser His Glu 
    1355                 1360                 1365             


Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala Lys Arg  Ala Arg Asp 
    1370                 1375                 1380             


Glu Arg  Glu Val Pro Val Gly  Ala Val Leu Val Leu  Asn Asn Arg 
    1385                 1390                 1395             


Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile Gly Leu  His Asp Pro 
    1400                 1405                 1410             


Thr Ala  His Ala Glu Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val 
    1415                 1420                 1425             


Met Gln  Asn Tyr Arg Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe 
    1430                 1435                 1440             


Glu Pro  Cys Val Met Cys Ala  Gly Ala Met Ile His  Ser Arg Ile 
    1445                 1450                 1455             


Gly Arg  Val Val Phe Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala 
    1460                 1465                 1470             


Gly Ser  Leu Met Asp Val Leu  His Tyr Pro Gly Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  315
<211>  1454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 124 Cas9 delta 792-872 
       TadAins polypeptide sequence

<400>  315

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Glu Val Glu Phe Ser His Glu 
785                 790                 795                 800 


Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu 
                805                 810                 815     


Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile 
            820                 825                 830         


Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His 
        835                 840                 845             


Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr 
    850                 855                 860                 


Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met 
865                 870                 875                 880 


Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly 
                885                 890                 895     


Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu 
            900                 905                 910         


His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu 
        915                 920                 925             


Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg 
    930                 935                 940                 


Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Glu Glu 
945                 950                 955                 960 


Val Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys 
                965                 970                 975     


Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly 
            980                 985                 990         


Gly Leu Ser Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val 
        995                 1000                 1005             


Glu Thr  Arg Gln Ile Thr Lys  His Val Ala Gln Ile  Leu Asp Ser 
    1010                 1015                 1020             


Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
    1025                 1030                 1035             


Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg 
    1040                 1045                 1050             


Lys Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His 
    1055                 1060                 1065             


His Ala  His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu 
    1070                 1075                 1080             


Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp 
    1085                 1090                 1095             


Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln 
    1100                 1105                 1110             


Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile 
    1115                 1120                 1125             


Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile 
    1130                 1135                 1140             


Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile 
    1145                 1150                 1155             


Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu 
    1160                 1165                 1170             


Ser Met  Pro Gln Val Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr 
    1175                 1180                 1185             


Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp 
    1190                 1195                 1200             


Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly 
    1205                 1210                 1215             


Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser Val Leu  Val Val Ala 
    1220                 1225                 1230             


Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu 
    1235                 1240                 1245             


Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn 
    1250                 1255                 1260             


Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
    1265                 1270                 1275             


Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu 
    1280                 1285                 1290             


Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys 
    1295                 1300                 1305             


Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr 
    1310                 1315                 1320             


Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn 
    1325                 1330                 1335             


Glu Gln  Lys Gln Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp 
    1340                 1345                 1350             


Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu 
    1355                 1360                 1365             


Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His 
    1370                 1375                 1380             


Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu 
    1385                 1390                 1395             


Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe 
    1400                 1405                 1410             


Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val 
    1415                 1420                 1425             


Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1430                 1435                 1440             


Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1445                 1450                 


<210>  316
<211>  1420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 125 Cas9 delta 792-906 
       TadAins polypeptide sequence

<400>  316

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Glu Val Glu Phe Ser His Glu 
785                 790                 795                 800 


Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu 
                805                 810                 815     


Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile 
            820                 825                 830         


Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His 
        835                 840                 845             


Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr 
    850                 855                 860                 


Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met 
865                 870                 875                 880 


Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly 
                885                 890                 895     


Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu 
            900                 905                 910         


His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu 
        915                 920                 925             


Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg 
    930                 935                 940                 


Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Gly Leu 
945                 950                 955                 960 


Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr 
                965                 970                 975     


Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn 
            980                 985                 990         


Thr Lys Tyr Asp Glu Asn Asp Lys  Leu Ile Arg Glu Val  Lys Val Ile 
        995                 1000                 1005             


Thr Leu  Lys Ser Lys Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln 
    1010                 1015                 1020             


Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr His His  Ala His Asp 
    1025                 1030                 1035             


Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr 
    1040                 1045                 1050             


Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr 
    1055                 1060                 1065             


Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys 
    1070                 1075                 1080             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1085                 1090                 1095             


Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro 
    1100                 1105                 1110             


Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys 
    1115                 1120                 1125             


Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val Leu Ser  Met Pro Gln 
    1130                 1135                 1140             


Val Asn  Ile Val Lys Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser 
    1145                 1150                 1155             


Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala 
    1160                 1165                 1170             


Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1175                 1180                 1185             


Pro Thr  Val Ala Tyr Ser Val  Leu Val Val Ala Lys  Val Glu Lys 
    1190                 1195                 1200             


Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile 
    1205                 1210                 1215             


Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe 
    1220                 1225                 1230             


Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile 
    1235                 1240                 1245             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys 
    1250                 1255                 1260             


Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu 
    1265                 1270                 1275             


Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His 
    1280                 1285                 1290             


Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln 
    1295                 1300                 1305             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1310                 1315                 1320             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1325                 1330                 1335             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1340                 1345                 1350             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1355                 1360                 1365             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1370                 1375                 1380             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1385                 1390                 1395             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1400                 1405                 1410             


Leu Ser  Gln Leu Gly Gly Asp  
    1415                 1420 


<210>  317
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 126 TadA CP65ins 1003 
       polypeptide sequence

<400>  317

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Thr Ala  His Ala Glu 
        995                 1000                 1005             


Ile Met  Ala Leu Arg Gln Gly  Gly Leu Val Met Gln  Asn Tyr Arg 
    1010                 1015                 1020             


Leu Ile  Asp Ala Thr Leu Tyr  Val Thr Phe Glu Pro  Cys Val Met 
    1025                 1030                 1035             


Cys Ala  Gly Ala Met Ile His  Ser Arg Ile Gly Arg  Val Val Phe 
    1040                 1045                 1050             


Gly Val  Arg Asn Ala Lys Thr  Gly Ala Ala Gly Ser  Leu Met Asp 
    1055                 1060                 1065             


Val Leu  His Tyr Pro Gly Met  Asn His Arg Val Glu  Ile Thr Glu 
    1070                 1075                 1080             


Gly Ile  Leu Ala Asp Glu Cys  Ala Ala Leu Leu Cys  Tyr Phe Phe 
    1085                 1090                 1095             


Arg Met  Pro Arg Gln Val Phe  Asn Ala Gln Lys Lys  Ala Gln Ser 
    1100                 1105                 1110             


Ser Thr  Asp Gly Ser Ser Gly  Ser Glu Thr Pro Gly  Thr Ser Glu 
    1115                 1120                 1125             


Ser Ala  Thr Pro Glu Ser Ser  Gly Ser Glu Val Glu  Phe Ser His 
    1130                 1135                 1140             


Glu Tyr  Trp Met Arg His Ala  Leu Thr Leu Ala Lys  Arg Ala Arg 
    1145                 1150                 1155             


Asp Glu  Arg Glu Val Pro Val  Gly Ala Val Leu Val  Leu Asn Asn 
    1160                 1165                 1170             


Arg Val  Ile Gly Glu Gly Trp  Asn Arg Ala Ile Gly  Leu His Asp 
    1175                 1180                 1185             


Pro Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1190                 1195                 1200             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  318
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 127 TadA CP65ins 1016 
       polypeptide sequence

<400>  318

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Thr Ala His Ala Glu  Ile Met Ala 
    1010                 1015                 1020             


Leu Arg  Gln Gly Gly Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp 
    1025                 1030                 1035             


Ala Thr  Leu Tyr Val Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly 
    1040                 1045                 1050             


Ala Met  Ile His Ser Arg Ile  Gly Arg Val Val Phe  Gly Val Arg 
    1055                 1060                 1065             


Asn Ala  Lys Thr Gly Ala Ala  Gly Ser Leu Met Asp  Val Leu His 
    1070                 1075                 1080             


Tyr Pro  Gly Met Asn His Arg  Val Glu Ile Thr Glu  Gly Ile Leu 
    1085                 1090                 1095             


Ala Asp  Glu Cys Ala Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro 
    1100                 1105                 1110             


Arg Gln  Val Phe Asn Ala Gln  Lys Lys Ala Gln Ser  Ser Thr Asp 
    1115                 1120                 1125             


Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr 
    1130                 1135                 1140             


Pro Glu  Ser Ser Gly Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp 
    1145                 1150                 1155             


Met Arg  His Ala Leu Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg 
    1160                 1165                 1170             


Glu Val  Pro Val Gly Ala Val  Leu Val Leu Asn Asn  Arg Val Ile 
    1175                 1180                 1185             


Gly Glu  Gly Trp Asn Arg Ala  Ile Gly Leu His Asp  Pro Tyr Asp 
    1190                 1195                 1200             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  319
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 128 TadA CP65ins 1022 
       polypeptide sequence

<400>  319

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Thr 
    1010                 1015                 1020             


Ala His  Ala Glu Ile Met Ala  Leu Arg Gln Gly Gly  Leu Val Met 
    1025                 1030                 1035             


Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu Tyr Val  Thr Phe Glu 
    1040                 1045                 1050             


Pro Cys  Val Met Cys Ala Gly  Ala Met Ile His Ser  Arg Ile Gly 
    1055                 1060                 1065             


Arg Val  Val Phe Gly Val Arg  Asn Ala Lys Thr Gly  Ala Ala Gly 
    1070                 1075                 1080             


Ser Leu  Met Asp Val Leu His  Tyr Pro Gly Met Asn  His Arg Val 
    1085                 1090                 1095             


Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu 
    1100                 1105                 1110             


Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val Phe Asn  Ala Gln Lys 
    1115                 1120                 1125             


Lys Ala  Gln Ser Ser Thr Asp  Gly Ser Ser Gly Ser  Glu Thr Pro 
    1130                 1135                 1140             


Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser Ser Gly  Ser Glu Val 
    1145                 1150                 1155             


Glu Phe  Ser His Glu Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala 
    1160                 1165                 1170             


Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro Val Gly  Ala Val Leu 
    1175                 1180                 1185             


Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile 
    1190                 1195                 1200             


Gly Leu  His Asp Pro Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  320
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 129 TadA CP65ins 1029 
       polypeptide sequence

<400>  320

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Thr  Ala His Ala Glu Ile  Met Ala Leu 
    1025                 1030                 1035             


Arg Gln  Gly Gly Leu Val Met  Gln Asn Tyr Arg Leu  Ile Asp Ala 
    1040                 1045                 1050             


Thr Leu  Tyr Val Thr Phe Glu  Pro Cys Val Met Cys  Ala Gly Ala 
    1055                 1060                 1065             


Met Ile  His Ser Arg Ile Gly  Arg Val Val Phe Gly  Val Arg Asn 
    1070                 1075                 1080             


Ala Lys  Thr Gly Ala Ala Gly  Ser Leu Met Asp Val  Leu His Tyr 
    1085                 1090                 1095             


Pro Gly  Met Asn His Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala 
    1100                 1105                 1110             


Asp Glu  Cys Ala Ala Leu Leu  Cys Tyr Phe Phe Arg  Met Pro Arg 
    1115                 1120                 1125             


Gln Val  Phe Asn Ala Gln Lys  Lys Ala Gln Ser Ser  Thr Asp Gly 
    1130                 1135                 1140             


Ser Ser  Gly Ser Glu Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro 
    1145                 1150                 1155             


Glu Ser  Ser Gly Ser Glu Val  Glu Phe Ser His Glu  Tyr Trp Met 
    1160                 1165                 1170             


Arg His  Ala Leu Thr Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu 
    1175                 1180                 1185             


Val Pro  Val Gly Ala Val Leu  Val Leu Asn Asn Arg  Val Ile Gly 
    1190                 1195                 1200             


Glu Gly  Trp Asn Arg Ala Ile  Gly Leu His Asp Pro  Gly Lys Ala 
    1205                 1210                 1215             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  321
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 130 TadA CP65ins 1041 
       polypeptide sequence

<400>  321

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Thr Ala His Ala Glu  Ile Met Ala Leu Arg  Gln Gly Gly 
    1040                 1045                 1050             


Leu Val  Met Gln Asn Tyr Arg  Leu Ile Asp Ala Thr  Leu Tyr Val 
    1055                 1060                 1065             


Thr Phe  Glu Pro Cys Val Met  Cys Ala Gly Ala Met  Ile His Ser 
    1070                 1075                 1080             


Arg Ile  Gly Arg Val Val Phe  Gly Val Arg Asn Ala  Lys Thr Gly 
    1085                 1090                 1095             


Ala Ala  Gly Ser Leu Met Asp  Val Leu His Tyr Pro  Gly Met Asn 
    1100                 1105                 1110             


His Arg  Val Glu Ile Thr Glu  Gly Ile Leu Ala Asp  Glu Cys Ala 
    1115                 1120                 1125             


Ala Leu  Leu Cys Tyr Phe Phe  Arg Met Pro Arg Gln  Val Phe Asn 
    1130                 1135                 1140             


Ala Gln  Lys Lys Ala Gln Ser  Ser Thr Asp Gly Ser  Ser Gly Ser 
    1145                 1150                 1155             


Glu Thr  Pro Gly Thr Ser Glu  Ser Ala Thr Pro Glu  Ser Ser Gly 
    1160                 1165                 1170             


Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp Met Arg  His Ala Leu 
    1175                 1180                 1185             


Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg Glu Val  Pro Val Gly 
    1190                 1195                 1200             


Ala Val  Leu Val Leu Asn Asn  Arg Val Ile Gly Glu  Gly Trp Asn 
    1205                 1210                 1215             


Arg Ala  Ile Gly Leu His Asp  Pro Asn Ile Met Asn  Phe Phe Lys 
    1220                 1225                 1230             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  322
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 131 TadA CP65ins 1054 
       polypeptide sequence

<400>  322

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Thr  Ala His Ala Glu Ile  Met Ala Leu Arg Gln  Gly Gly Leu 
    1055                 1060                 1065             


Val Met  Gln Asn Tyr Arg Leu  Ile Asp Ala Thr Leu  Tyr Val Thr 
    1070                 1075                 1080             


Phe Glu  Pro Cys Val Met Cys  Ala Gly Ala Met Ile  His Ser Arg 
    1085                 1090                 1095             


Ile Gly  Arg Val Val Phe Gly  Val Arg Asn Ala Lys  Thr Gly Ala 
    1100                 1105                 1110             


Ala Gly  Ser Leu Met Asp Val  Leu His Tyr Pro Gly  Met Asn His 
    1115                 1120                 1125             


Arg Val  Glu Ile Thr Glu Gly  Ile Leu Ala Asp Glu  Cys Ala Ala 
    1130                 1135                 1140             


Leu Leu  Cys Tyr Phe Phe Arg  Met Pro Arg Gln Val  Phe Asn Ala 
    1145                 1150                 1155             


Gln Lys  Lys Ala Gln Ser Ser  Thr Asp Gly Ser Ser  Gly Ser Glu 
    1160                 1165                 1170             


Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly Ser 
    1175                 1180                 1185             


Glu Val  Glu Phe Ser His Glu  Tyr Trp Met Arg His  Ala Leu Thr 
    1190                 1195                 1200             


Leu Ala  Lys Arg Ala Arg Asp  Glu Arg Glu Val Pro  Val Gly Ala 
    1205                 1210                 1215             


Val Leu  Val Leu Asn Asn Arg  Val Ile Gly Glu Gly  Trp Asn Arg 
    1220                 1225                 1230             


Ala Ile  Gly Leu His Asp Pro  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1235                 1240                 1245             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1250                 1255                 1260             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1265                 1270                 1275             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1280                 1285                 1290             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1295                 1300                 1305             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1310                 1315                 1320             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1325                 1330                 1335             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1340                 1345                 1350             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1355                 1360                 1365             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1370                 1375                 1380             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1385                 1390                 1395             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1400                 1405                 1410             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1415                 1420                 1425             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  323
<211>  1554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 132 TadA CP65ins 1246 
       polypeptide sequence

<400>  323

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Thr 
    1235                 1240                 1245             


Ala His  Ala Glu Ile Met Ala  Leu Arg Gln Gly Gly  Leu Val Met 
    1250                 1255                 1260             


Gln Asn  Tyr Arg Leu Ile Asp  Ala Thr Leu Tyr Val  Thr Phe Glu 
    1265                 1270                 1275             


Pro Cys  Val Met Cys Ala Gly  Ala Met Ile His Ser  Arg Ile Gly 
    1280                 1285                 1290             


Arg Val  Val Phe Gly Val Arg  Asn Ala Lys Thr Gly  Ala Ala Gly 
    1295                 1300                 1305             


Ser Leu  Met Asp Val Leu His  Tyr Pro Gly Met Asn  His Arg Val 
    1310                 1315                 1320             


Glu Ile  Thr Glu Gly Ile Leu  Ala Asp Glu Cys Ala  Ala Leu Leu 
    1325                 1330                 1335             


Cys Tyr  Phe Phe Arg Met Pro  Arg Gln Val Phe Asn  Ala Gln Lys 
    1340                 1345                 1350             


Lys Ala  Gln Ser Ser Thr Asp  Gly Ser Ser Gly Ser  Glu Thr Pro 
    1355                 1360                 1365             


Gly Thr  Ser Glu Ser Ala Thr  Pro Glu Ser Ser Gly  Ser Glu Val 
    1370                 1375                 1380             


Glu Phe  Ser His Glu Tyr Trp  Met Arg His Ala Leu  Thr Leu Ala 
    1385                 1390                 1395             


Lys Arg  Ala Arg Asp Glu Arg  Glu Val Pro Val Gly  Ala Val Leu 
    1400                 1405                 1410             


Val Leu  Asn Asn Arg Val Ile  Gly Glu Gly Trp Asn  Arg Ala Ile 
    1415                 1420                 1425             


Gly Leu  His Asp Pro Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1430                 1435                 1440             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1445                 1450                 1455             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1460                 1465                 1470             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1475                 1480                 1485             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1490                 1495                 1500             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1505                 1510                 1515             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1520                 1525                 1530             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1535                 1540                 1545             


Ser Gln  Leu Gly Gly Asp 
    1550                 


<210>  324
<211>  160
<212>  PRT
<213>  Staphylococcus aureus


<220>
<221>  MISC_FEATURE
<222>  (1)..(160)
<223>  TadA polypeptide sequence

<400>  324

Met Gly Ser His Met Thr Asn Asp Ile Tyr Phe Met Thr Leu Ala Ile 
1               5                   10                  15      


Glu Glu Ala Lys Lys Ala Ala Gln Leu Gly Glu Val Pro Ile Gly Ala 
            20                  25                  30          


Ile Ile Thr Lys Asp Asp Glu Val Ile Ala Arg Ala His Asn Leu Arg 
        35                  40                  45              


Glu Thr Leu Gln Gln Pro Thr Ala His Ala Glu His Ile Ala Ile Glu 
    50                  55                  60                  


Arg Ala Ala Lys Val Leu Gly Ser Trp Arg Leu Glu Gly Cys Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Thr Ile Val Met 
                85                  90                  95      


Ser Arg Ile Pro Arg Val Val Tyr Gly Ala Asp Asp Pro Lys Gly Gly 
            100                 105                 110         


Cys Ser Gly Ser Leu Met Asn Leu Leu Gln Gln Ser Asn Phe Asn His 
        115                 120                 125             


Arg Ala Ile Val Asp Lys Gly Val Leu Lys Glu Ala Cys Ser Thr Leu 
    130                 135                 140                 


Leu Thr Thr Phe Phe Lys Asn Leu Arg Ala Asn Lys Lys Ser Thr Asn 
145                 150                 155                 160 


<210>  325
<211>  161
<212>  PRT
<213>  Bacillus subtilis


<220>
<221>  MISC_FEATURE
<222>  (1)..(161)
<223>  TadA polypeptide sequence

<400>  325

Met Thr Gln Asp Glu Leu Tyr Met Lys Glu Ala Ile Lys Glu Ala Lys 
1               5                   10                  15      


Lys Ala Glu Glu Lys Gly Glu Val Pro Ile Gly Ala Val Leu Val Ile 
            20                  25                  30          


Asn Gly Glu Ile Ile Ala Arg Ala His Asn Leu Arg Glu Thr Glu Gln 
        35                  40                  45              


Arg Ser Ile Ala His Ala Glu Met Leu Val Ile Asp Glu Ala Cys Lys 
    50                  55                  60                  


Ala Leu Gly Thr Trp Arg Leu Glu Gly Ala Thr Leu Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Pro Met Cys Ala Gly Ala Val Val Leu Ser Arg Val Glu 
                85                  90                  95      


Lys Val Val Phe Gly Ala Phe Asp Pro Lys Gly Gly Cys Ser Gly Thr 
            100                 105                 110         


Leu Met Asn Leu Leu Gln Glu Glu Arg Phe Asn His Gln Ala Glu Val 
        115                 120                 125             


Val Ser Gly Val Leu Glu Glu Glu Cys Gly Gly Met Leu Ser Ala Phe 
    130                 135                 140                 


Phe Arg Glu Leu Arg Lys Lys Lys Lys Ala Ala Arg Lys Asn Leu Ser 
145                 150                 155                 160 


Glu 
    


<210>  326
<211>  183
<212>  PRT
<213>  Salmonella typhimurium


<220>
<221>  MISC_FEATURE
<222>  (1)..(183)
<223>  TadA polypeptide sequence

<400>  326

Met Pro Pro Ala Phe Ile Thr Gly Val Thr Ser Leu Ser Asp Val Glu 
1               5                   10                  15      


Leu Asp His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg 
            20                  25                  30          


Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val His Asn 
        35                  40                  45              


His Arg Val Ile Gly Glu Gly Trp Asn Arg Pro Ile Gly Arg His Asp 
    50                  55                  60                  


Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val 
65                  70                  75                  80  


Leu Gln Asn Tyr Arg Leu Leu Asp Thr Thr Leu Tyr Val Thr Leu Glu 
                85                  90                  95      


Pro Cys Val Met Cys Ala Gly Ala Met Val His Ser Arg Ile Gly Arg 
            100                 105                 110         


Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly Ala Ala Gly Ser Leu 
        115                 120                 125             


Ile Asp Val Leu His His Pro Gly Met Asn His Arg Val Glu Ile Ile 
    130                 135                 140                 


Glu Gly Val Leu Arg Asp Glu Cys Ala Thr Leu Leu Ser Asp Phe Phe 
145                 150                 155                 160 


Arg Met Arg Arg Gln Glu Ile Lys Ala Leu Lys Lys Ala Asp Arg Ala 
                165                 170                 175     


Glu Gly Ala Gly Pro Ala Val 
            180             


<210>  327
<211>  164
<212>  PRT
<213>  Shewanella putrefaciens


<220>
<221>  MISC_FEATURE
<222>  (1)..(164)
<223>  TadA polypeptide sequence

<400>  327

Met Asp Glu Tyr Trp Met Gln Val Ala Met Gln Met Ala Glu Lys Ala 
1               5                   10                  15      


Glu Ala Ala Gly Glu Val Pro Val Gly Ala Val Leu Val Lys Asp Gly 
            20                  25                  30          


Gln Gln Ile Ala Thr Gly Tyr Asn Leu Ser Ile Ser Gln His Asp Pro 
        35                  40                  45              


Thr Ala His Ala Glu Ile Leu Cys Leu Arg Ser Ala Gly Lys Lys Leu 
    50                  55                  60                  


Glu Asn Tyr Arg Leu Leu Asp Ala Thr Leu Tyr Ile Thr Leu Glu Pro 
65                  70                  75                  80  


Cys Ala Met Cys Ala Gly Ala Met Val His Ser Arg Ile Ala Arg Val 
                85                  90                  95      


Val Tyr Gly Ala Arg Asp Glu Lys Thr Gly Ala Ala Gly Thr Val Val 
            100                 105                 110         


Asn Leu Leu Gln His Pro Ala Phe Asn His Gln Val Glu Val Thr Ser 
        115                 120                 125             


Gly Val Leu Ala Glu Ala Cys Ser Ala Gln Leu Ser Arg Phe Phe Lys 
    130                 135                 140                 


Arg Arg Arg Asp Glu Lys Lys Ala Leu Lys Leu Ala Gln Arg Ala Gln 
145                 150                 155                 160 


Gln Gly Ile Glu 
                


<210>  328
<211>  173
<212>  PRT
<213>  Haemophilus influenzae


<220>
<221>  MISC_FEATURE
<222>  (1)..(173)
<223>  TadA polypeptide sequence

<400>  328

Met Asp Ala Ala Lys Val Arg Ser Glu Phe Asp Glu Lys Met Met Arg 
1               5                   10                  15      


Tyr Ala Leu Glu Leu Ala Asp Lys Ala Glu Ala Leu Gly Glu Ile Pro 
            20                  25                  30          


Val Gly Ala Val Leu Val Asp Asp Ala Arg Asn Ile Ile Gly Glu Gly 
        35                  40                  45              


Trp Asn Leu Ser Ile Val Gln Ser Asp Pro Thr Ala His Ala Glu Ile 
    50                  55                  60                  


Ile Ala Leu Arg Asn Gly Ala Lys Asn Ile Gln Asn Tyr Arg Leu Leu 
65                  70                  75                  80  


Asn Ser Thr Leu Tyr Val Thr Leu Glu Pro Cys Thr Met Cys Ala Gly 
                85                  90                  95      


Ala Ile Leu His Ser Arg Ile Lys Arg Leu Val Phe Gly Ala Ser Asp 
            100                 105                 110         


Tyr Lys Thr Gly Ala Ile Gly Ser Arg Phe His Phe Phe Asp Asp Tyr 
        115                 120                 125             


Lys Met Asn His Thr Leu Glu Ile Thr Ser Gly Val Leu Ala Glu Glu 
    130                 135                 140                 


Cys Ser Gln Lys Leu Ser Thr Phe Phe Gln Lys Arg Arg Glu Glu Lys 
145                 150                 155                 160 


Lys Ile Glu Lys Ala Leu Leu Lys Ser Leu Ser Asp Lys 
                165                 170             


<210>  329
<211>  161
<212>  PRT
<213>  Caulobacter crescentus


<220>
<221>  MISC_FEATURE
<222>  (1)..(161)
<223>  TadA polypeptide sequence

<400>  329

Met Arg Thr Asp Glu Ser Glu Asp Gln Asp His Arg Met Met Arg Leu 
1               5                   10                  15      


Ala Leu Asp Ala Ala Arg Ala Ala Ala Glu Ala Gly Glu Thr Pro Val 
            20                  25                  30          


Gly Ala Val Ile Leu Asp Pro Ser Thr Gly Glu Val Ile Ala Thr Ala 
        35                  40                  45              


Gly Asn Gly Pro Ile Ala Ala His Asp Pro Thr Ala His Ala Glu Ile 
    50                  55                  60                  


Ala Ala Met Arg Ala Ala Ala Ala Lys Leu Gly Asn Tyr Arg Leu Thr 
65                  70                  75                  80  


Asp Leu Thr Leu Val Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly 
                85                  90                  95      


Ala Ile Ser His Ala Arg Ile Gly Arg Val Val Phe Gly Ala Asp Asp 
            100                 105                 110         


Pro Lys Gly Gly Ala Val Val His Gly Pro Lys Phe Phe Ala Gln Pro 
        115                 120                 125             


Thr Cys His Trp Arg Pro Glu Val Thr Gly Gly Val Leu Ala Asp Glu 
    130                 135                 140                 


Ser Ala Asp Leu Leu Arg Gly Phe Phe Arg Ala Arg Arg Lys Ala Lys 
145                 150                 155                 160 


Ile 
    


<210>  330
<211>  179
<212>  PRT
<213>  Geobacter sulfurreducens


<220>
<221>  MISC_FEATURE
<222>  (1)..(179)
<223>  TadA polypeptide sequence

<400>  330

Met Ser Ser Leu Lys Lys Thr Pro Ile Arg Asp Asp Ala Tyr Trp Met 
1               5                   10                  15      


Gly Lys Ala Ile Arg Glu Ala Ala Lys Ala Ala Ala Arg Asp Glu Val 
            20                  25                  30          


Pro Ile Gly Ala Val Ile Val Arg Asp Gly Ala Val Ile Gly Arg Gly 
        35                  40                  45              


His Asn Leu Arg Glu Gly Ser Asn Asp Pro Ser Ala His Ala Glu Met 
    50                  55                  60                  


Ile Ala Ile Arg Gln Ala Ala Arg Arg Ser Ala Asn Trp Arg Leu Thr 
65                  70                  75                  80  


Gly Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Leu Met Cys Met Gly 
                85                  90                  95      


Ala Ile Ile Leu Ala Arg Leu Glu Arg Val Val Phe Gly Cys Tyr Asp 
            100                 105                 110         


Pro Lys Gly Gly Ala Ala Gly Ser Leu Tyr Asp Leu Ser Ala Asp Pro 
        115                 120                 125             


Arg Leu Asn His Gln Val Arg Leu Ser Pro Gly Val Cys Gln Glu Glu 
    130                 135                 140                 


Cys Gly Thr Met Leu Ser Asp Phe Phe Arg Asp Leu Arg Arg Arg Lys 
145                 150                 155                 160 


Lys Ala Lys Ala Thr Pro Ala Leu Phe Ile Asp Glu Arg Lys Val Pro 
                165                 170                 175     


Pro Glu Pro 
            


<210>  331
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: TadA*8 polypeptide sequence

<400>  331

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Thr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp 
                165         


<210>  332
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: gRNA scaffold polynucleotide 
       sequence

<400>  332
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


<210>  333
<211>  125
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: gRNA scaffold polynucleotide 
       sequence

<400>  333
guuuuuguac ucucaagauu uaaguaacug uacaacgaaa cuuacacagu uacuuaaauc       60

uugcagaagc uacaaagaua aggcuucaug ccgaaaucaa cacccuguca uuuuauggca      120

gggug                                                                  125


<210>  334
<211>  76
<212>  RNA
<213>  S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(76)
<223>  S. pyogenes gRNA scaffold ologonucleotide sequence

<400>  334
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugc                                                       76


<210>  335
<211>  84
<212>  RNA
<213>  S. aureus


<220>
<221>  misc_feature
<222>  (1)..(84)
<223>  S. aureus gRNA scaffold oligonucleotide sequence

<400>  335
guuuuaguac ucuguaauga aaauuacaga aucuacuaaa acaaggcaaa augccguguu       60

uaucucguca acuuguuggc gaga                                              84


<210>  336
<211>  97
<212>  RNA
<213>  Bacillus hisashii


<220>
<221>  misc_feature
<222>  (1)..(97)
<223>  BhCas12b gRNA scaffold oligonucleotide sequence

<400>  336
guucugucuu uuggucagga caaccgucua gcuauaagug cugcagggug ugagaaacuc       60

cuauugcugg acgaugucuc uuacgaggca uuagcac                                97


<210>  337
<211>  88
<212>  RNA
<213>  Bacillus sp.


<220>
<221>  misc_feature
<222>  (1)..(88)
<223>  BvCas12b gRNA scaffold oligonucleotide sequence

<400>  337
gaccuauagg gucaaugaau cugugcgugu gccauaagua auuaaaaauu acccaccaca       60

ggagcaccug aaaacaggug cuuggcac                                          88


<210>  338
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: gRNA scaffold nucleotide 
       sequence; oligonucleotide sequence


<220>
<221>  modified_base
<222>  (78)..(80)
<223>  um

<220>
<221>  misc_feature
<222>  (78)..(80)
<223>  In embodiments, a nucleotide(s) is linked to the preceding 5' 
       nucleotide by a 3' phosphorothioate internucleotide liknage

<400>  338
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

gggaccgagu cggugcuuuu                                                   80


<210>  339
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: gRNA scaffold nucleotide 
       sequence


<220>
<221>  misc_feature
<222>  (78)..(80)
<223>  In embodiments, a nucleotide(s) is linked to the preceding 5' 
       nucleotide by a 3' phosphorothioate internucleotide liknage

<220>
<221>  modified_base
<222>  (78)..(80)
<223>  um

<400>  339
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


<210>  340
<211>  117
<212>  RNA
<213>  Bacillus hisashii


<220>
<221>  misc_feature
<222>  (1)..(117)
<223>  BhCas12b gRNA scaffold + guide sequence; oligonucleotide sequence

<220>
<221>  misc_feature
<222>  (1)..(97)
<223>  BhCas12b sgRNA scaffold

<400>  340
guucugucuu uuggucagga caaccgucua gcuauaagug cugcagggug ugagaaacuc       60

cuauugcugg acgaugucuc uuacgaggca uuagcacnnn nnnnnnnnnn nnnnnnn         117


<210>  341
<211>  108
<212>  RNA
<213>  Bacillus sp.


<220>
<221>  misc_feature
<222>  (1)..(108)
<223>  BvCas12b gRNA scaffold + guide sequence; oligonucleotide sequence

<220>
<221>  misc_feature
<222>  (1)..(88)
<223>  BvCas12b gRNA scaffold

<400>  341
gaccuauagg gucaaugaau cugugcgugu gccauaagua auuaaaaauu acccaccaca       60

ggagcaccug aaaacaggug cuuggcacnn nnnnnnnnnn nnnnnnnn                   108


<210>  342
<211>  119
<212>  RNA
<213>  Alicyclobacillus acidiphilus


<220>
<221>  misc_feature
<222>  (1)..(119)
<223>  AaCas12b gRNA scaffold + guide sequence; oligonucleotide sequence

<220>
<221>  misc_feature
<222>  (1)..(99)
<223>  AaCas12b gRNA scaffold

<400>  342
gucuaaagga cagaauuuuu caacgggugu gccaauggcc acuuuccagg uggcaaagcc       60

cguugaacuu cucaaaaaga acgaucugag aaguggcacn nnnnnnnnnn nnnnnnnnn       119


<210>  343
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  343

Pro Lys Lys Lys Arg Lys Val Glu Gly Ala Asp Lys Arg Thr Ala Asp 
1               5                   10                  15      


Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg Lys Val 
            20                  25                  


<210>  344
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  344

Arg Lys Ser Gly Lys Ile Ala Ala Ile Val Val Lys Arg Pro Arg Lys 
1               5                   10                  15      


Pro Lys Lys Lys Arg Lys Val 
            20              


<210>  345
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  345

Ser Gly Gly Ser Ser Gly Gly Ser 
1               5               


<210>  346
<211>  1609
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pNMG-B335 
       ABE8.1_Y147T_CP5_NGC PAM_monomer polypeptide sequence

<400>  346

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Thr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Glu Ile Gly Lys Ala Thr Ala Lys Tyr 
        195                 200                 205             


Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu 
    210                 215                 220                 


Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu 
225                 230                 235                 240 


Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg 
                245                 250                 255     


Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val 
            260                 265                 270         


Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser 
        275                 280                 285             


Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly 
    290                 295                 300                 


Gly Phe Met Gln Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys 
305                 310                 315                 320 


Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu 
                325                 330                 335     


Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp 
            340                 345                 350         


Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile 
        355                 360                 365             


Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg 
    370                 375                 380                 


Met Leu Ala Ser Ala Lys Phe Leu Gln Lys Gly Asn Glu Leu Ala Leu 
385                 390                 395                 400 


Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys 
                405                 410                 415     


Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu 
            420                 425                 430         


Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe 
        435                 440                 445             


Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser 
    450                 455                 460                 


Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn 
465                 470                 475                 480 


Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Arg Ala Phe 
                485                 490                 495     


Lys Tyr Phe Asp Thr Thr Ile Ala Arg Lys Glu Tyr Arg Ser Thr Lys 
            500                 505                 510         


Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        515                 520                 525             


Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Gly Gly Ser Gly 
    530                 535                 540                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
545                 550                 555                 560 


Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
                565                 570                 575     


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            580                 585                 590         


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        595                 600                 605             


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    610                 615                 620                 


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
625                 630                 635                 640 


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                645                 650                 655     


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            660                 665                 670         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        675                 680                 685             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    690                 695                 700                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
705                 710                 715                 720 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                725                 730                 735     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            740                 745                 750         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        755                 760                 765             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    770                 775                 780                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
785                 790                 795                 800 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                805                 810                 815     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            820                 825                 830         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        835                 840                 845             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    850                 855                 860                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
865                 870                 875                 880 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                885                 890                 895     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            900                 905                 910         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        915                 920                 925             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    930                 935                 940                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
945                 950                 955                 960 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                965                 970                 975     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            980                 985                 990         


Leu Lys Asp Asn Arg Glu Lys Ile  Glu Lys Ile Leu Thr  Phe Arg Ile 
        995                 1000                 1005             


Pro Tyr  Tyr Val Gly Pro Leu  Ala Arg Gly Asn Ser  Arg Phe Ala 
    1010                 1015                 1020             


Trp Met  Thr Arg Lys Ser Glu  Glu Thr Ile Thr Pro  Trp Asn Phe 
    1025                 1030                 1035             


Glu Glu  Val Val Asp Lys Gly  Ala Ser Ala Gln Ser  Phe Ile Glu 
    1040                 1045                 1050             


Arg Met  Thr Asn Phe Asp Lys  Asn Leu Pro Asn Glu  Lys Val Leu 
    1055                 1060                 1065             


Pro Lys  His Ser Leu Leu Tyr  Glu Tyr Phe Thr Val  Tyr Asn Glu 
    1070                 1075                 1080             


Leu Thr  Lys Val Lys Tyr Val  Thr Glu Gly Met Arg  Lys Pro Ala 
    1085                 1090                 1095             


Phe Leu  Ser Gly Glu Gln Lys  Lys Ala Ile Val Asp  Leu Leu Phe 
    1100                 1105                 1110             


Lys Thr  Asn Arg Lys Val Thr  Val Lys Gln Leu Lys  Glu Asp Tyr 
    1115                 1120                 1125             


Phe Lys  Lys Ile Glu Cys Phe  Asp Ser Val Glu Ile  Ser Gly Val 
    1130                 1135                 1140             


Glu Asp  Arg Phe Asn Ala Ser  Leu Gly Thr Tyr His  Asp Leu Leu 
    1145                 1150                 1155             


Lys Ile  Ile Lys Asp Lys Asp  Phe Leu Asp Asn Glu  Glu Asn Glu 
    1160                 1165                 1170             


Asp Ile  Leu Glu Asp Ile Val  Leu Thr Leu Thr Leu  Phe Glu Asp 
    1175                 1180                 1185             


Arg Glu  Met Ile Glu Glu Arg  Leu Lys Thr Tyr Ala  His Leu Phe 
    1190                 1195                 1200             


Asp Asp  Lys Val Met Lys Gln  Leu Lys Arg Arg Arg  Tyr Thr Gly 
    1205                 1210                 1215             


Trp Gly  Arg Leu Ser Arg Lys  Leu Ile Asn Gly Ile  Arg Asp Lys 
    1220                 1225                 1230             


Gln Ser  Gly Lys Thr Ile Leu  Asp Phe Leu Lys Ser  Asp Gly Phe 
    1235                 1240                 1245             


Ala Asn  Arg Asn Phe Met Gln  Leu Ile His Asp Asp  Ser Leu Thr 
    1250                 1255                 1260             


Phe Lys  Glu Asp Ile Gln Lys  Ala Gln Val Ser Gly  Gln Gly Asp 
    1265                 1270                 1275             


Ser Leu  His Glu His Ile Ala  Asn Leu Ala Gly Ser  Pro Ala Ile 
    1280                 1285                 1290             


Lys Lys  Gly Ile Leu Gln Thr  Val Lys Val Val Asp  Glu Leu Val 
    1295                 1300                 1305             


Lys Val  Met Gly Arg His Lys  Pro Glu Asn Ile Val  Ile Glu Met 
    1310                 1315                 1320             


Ala Arg  Glu Asn Gln Thr Thr  Gln Lys Gly Gln Lys  Asn Ser Arg 
    1325                 1330                 1335             


Glu Arg  Met Lys Arg Ile Glu  Glu Gly Ile Lys Glu  Leu Gly Ser 
    1340                 1345                 1350             


Gln Ile  Leu Lys Glu His Pro  Val Glu Asn Thr Gln  Leu Gln Asn 
    1355                 1360                 1365             


Glu Lys  Leu Tyr Leu Tyr Tyr  Leu Gln Asn Gly Arg  Asp Met Tyr 
    1370                 1375                 1380             


Val Asp  Gln Glu Leu Asp Ile  Asn Arg Leu Ser Asp  Tyr Asp Val 
    1385                 1390                 1395             


Asp His  Ile Val Pro Gln Ser  Phe Leu Lys Asp Asp  Ser Ile Asp 
    1400                 1405                 1410             


Asn Lys  Val Leu Thr Arg Ser  Asp Lys Asn Arg Gly  Lys Ser Asp 
    1415                 1420                 1425             


Asn Val  Pro Ser Glu Glu Val  Val Lys Lys Met Lys  Asn Tyr Trp 
    1430                 1435                 1440             


Arg Gln  Leu Leu Asn Ala Lys  Leu Ile Thr Gln Arg  Lys Phe Asp 
    1445                 1450                 1455             


Asn Leu  Thr Lys Ala Glu Arg  Gly Gly Leu Ser Glu  Leu Asp Lys 
    1460                 1465                 1470             


Ala Gly  Phe Ile Lys Arg Gln  Leu Val Glu Thr Arg  Gln Ile Thr 
    1475                 1480                 1485             


Lys His  Val Ala Gln Ile Leu  Asp Ser Arg Met Asn  Thr Lys Tyr 
    1490                 1495                 1500             


Asp Glu  Asn Asp Lys Leu Ile  Arg Glu Val Lys Val  Ile Thr Leu 
    1505                 1510                 1515             


Lys Ser  Lys Leu Val Ser Asp  Phe Arg Lys Asp Phe  Gln Phe Tyr 
    1520                 1525                 1530             


Lys Val  Arg Glu Ile Asn Asn  Tyr His His Ala His  Asp Ala Tyr 
    1535                 1540                 1545             


Leu Asn  Ala Val Val Gly Thr  Ala Leu Ile Lys Lys  Tyr Pro Lys 
    1550                 1555                 1560             


Leu Glu  Ser Glu Phe Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val 
    1565                 1570                 1575             


Arg Lys  Met Ile Ala Lys Ser  Glu Gln Glu Gly Ala  Asp Lys Arg 
    1580                 1585                 1590             


Thr Ala  Asp Gly Ser Glu Phe  Glu Ser Pro Lys Lys  Lys Arg Lys 
    1595                 1600                 1605             


Val 
    


<210>  347
<211>  1807
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: pNMG-357_ABE8.14 with NGC PAM
       CP5 polypeptide sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(174)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (175)..(198)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (199)..(365)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (366)..(397)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (398)..(738)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (739)..(759)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (760)..(1785)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1786)..(1807)
<223>  Bipartite nuclear localization sequence

<400>  347

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Gly Gly Ser Ser Gly Gly Ser Ser Gly 
                165                 170                 175     


Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly 
            180                 185                 190         


Gly Ser Ser Gly Gly Ser Met Ser Glu Val Glu Phe Ser His Glu Tyr 
        195                 200                 205             


Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg 
    210                 215                 220                 


Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly 
225                 230                 235                 240 


Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala 
                245                 250                 255     


Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg 
            260                 265                 270         


Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys 
        275                 280                 285             


Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val 
    290                 295                 300                 


Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His 
305                 310                 315                 320 


Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala 
                325                 330                 335     


Asp Glu Cys Ala Ala Leu Leu Cys Thr Phe Phe Arg Met Pro Arg Gln 
            340                 345                 350         


Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly 
        355                 360                 365             


Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
    370                 375                 380                 


Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Glu Ile Gly 
385                 390                 395                 400 


Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe 
                405                 410                 415     


Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu 
            420                 425                 430         


Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg 
        435                 440                 445             


Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile 
    450                 455                 460                 


Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile 
465                 470                 475                 480 


Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp 
                485                 490                 495     


Asp Pro Lys Lys Tyr Gly Gly Phe Met Gln Pro Thr Val Ala Tyr Ser 
            500                 505                 510         


Val Leu Val Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys 
        515                 520                 525             


Ser Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe 
    530                 535                 540                 


Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val 
545                 550                 555                 560 


Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu 
                565                 570                 575     


Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Lys Phe Leu Gln Lys 
            580                 585                 590         


Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu 
        595                 600                 605             


Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln 
    610                 615                 620                 


Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile 
625                 630                 635                 640 


Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn 
                645                 650                 655     


Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile 
            660                 665                 670         


Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn Leu 
        675                 680                 685             


Gly Ala Pro Arg Ala Phe Lys Tyr Phe Asp Thr Thr Ile Ala Arg Lys 
    690                 695                 700                 


Glu Tyr Arg Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln 
705                 710                 715                 720 


Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly 
                725                 730                 735     


Gly Asp Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            740                 745                 750         


Ser Gly Gly Ser Gly Gly Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        755                 760                 765             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    770                 775                 780                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
785                 790                 795                 800 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                805                 810                 815     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            820                 825                 830         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        835                 840                 845             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    850                 855                 860                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
865                 870                 875                 880 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                885                 890                 895     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            900                 905                 910         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        915                 920                 925             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    930                 935                 940                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
945                 950                 955                 960 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                965                 970                 975     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            980                 985                 990         


Asn Gly Leu Phe Gly Asn Leu Ile  Ala Leu Ser Leu Gly  Leu Thr Pro 
        995                 1000                 1005             


Asn Phe  Lys Ser Asn Phe Asp  Leu Ala Glu Asp Ala  Lys Leu Gln 
    1010                 1015                 1020             


Leu Ser  Lys Asp Thr Tyr Asp  Asp Asp Leu Asp Asn  Leu Leu Ala 
    1025                 1030                 1035             


Gln Ile  Gly Asp Gln Tyr Ala  Asp Leu Phe Leu Ala  Ala Lys Asn 
    1040                 1045                 1050             


Leu Ser  Asp Ala Ile Leu Leu  Ser Asp Ile Leu Arg  Val Asn Thr 
    1055                 1060                 1065             


Glu Ile  Thr Lys Ala Pro Leu  Ser Ala Ser Met Ile  Lys Arg Tyr 
    1070                 1075                 1080             


Asp Glu  His His Gln Asp Leu  Thr Leu Leu Lys Ala  Leu Val Arg 
    1085                 1090                 1095             


Gln Gln  Leu Pro Glu Lys Tyr  Lys Glu Ile Phe Phe  Asp Gln Ser 
    1100                 1105                 1110             


Lys Asn  Gly Tyr Ala Gly Tyr  Ile Asp Gly Gly Ala  Ser Gln Glu 
    1115                 1120                 1125             


Glu Phe  Tyr Lys Phe Ile Lys  Pro Ile Leu Glu Lys  Met Asp Gly 
    1130                 1135                 1140             


Thr Glu  Glu Leu Leu Val Lys  Leu Asn Arg Glu Asp  Leu Leu Arg 
    1145                 1150                 1155             


Lys Gln  Arg Thr Phe Asp Asn  Gly Ser Ile Pro His  Gln Ile His 
    1160                 1165                 1170             


Leu Gly  Glu Leu His Ala Ile  Leu Arg Arg Gln Glu  Asp Phe Tyr 
    1175                 1180                 1185             


Pro Phe  Leu Lys Asp Asn Arg  Glu Lys Ile Glu Lys  Ile Leu Thr 
    1190                 1195                 1200             


Phe Arg  Ile Pro Tyr Tyr Val  Gly Pro Leu Ala Arg  Gly Asn Ser 
    1205                 1210                 1215             


Arg Phe  Ala Trp Met Thr Arg  Lys Ser Glu Glu Thr  Ile Thr Pro 
    1220                 1225                 1230             


Trp Asn  Phe Glu Glu Val Val  Asp Lys Gly Ala Ser  Ala Gln Ser 
    1235                 1240                 1245             


Phe Ile  Glu Arg Met Thr Asn  Phe Asp Lys Asn Leu  Pro Asn Glu 
    1250                 1255                 1260             


Lys Val  Leu Pro Lys His Ser  Leu Leu Tyr Glu Tyr  Phe Thr Val 
    1265                 1270                 1275             


Tyr Asn  Glu Leu Thr Lys Val  Lys Tyr Val Thr Glu  Gly Met Arg 
    1280                 1285                 1290             


Lys Pro  Ala Phe Leu Ser Gly  Glu Gln Lys Lys Ala  Ile Val Asp 
    1295                 1300                 1305             


Leu Leu  Phe Lys Thr Asn Arg  Lys Val Thr Val Lys  Gln Leu Lys 
    1310                 1315                 1320             


Glu Asp  Tyr Phe Lys Lys Ile  Glu Cys Phe Asp Ser  Val Glu Ile 
    1325                 1330                 1335             


Ser Gly  Val Glu Asp Arg Phe  Asn Ala Ser Leu Gly  Thr Tyr His 
    1340                 1345                 1350             


Asp Leu  Leu Lys Ile Ile Lys  Asp Lys Asp Phe Leu  Asp Asn Glu 
    1355                 1360                 1365             


Glu Asn  Glu Asp Ile Leu Glu  Asp Ile Val Leu Thr  Leu Thr Leu 
    1370                 1375                 1380             


Phe Glu  Asp Arg Glu Met Ile  Glu Glu Arg Leu Lys  Thr Tyr Ala 
    1385                 1390                 1395             


His Leu  Phe Asp Asp Lys Val  Met Lys Gln Leu Lys  Arg Arg Arg 
    1400                 1405                 1410             


Tyr Thr  Gly Trp Gly Arg Leu  Ser Arg Lys Leu Ile  Asn Gly Ile 
    1415                 1420                 1425             


Arg Asp  Lys Gln Ser Gly Lys  Thr Ile Leu Asp Phe  Leu Lys Ser 
    1430                 1435                 1440             


Asp Gly  Phe Ala Asn Arg Asn  Phe Met Gln Leu Ile  His Asp Asp 
    1445                 1450                 1455             


Ser Leu  Thr Phe Lys Glu Asp  Ile Gln Lys Ala Gln  Val Ser Gly 
    1460                 1465                 1470             


Gln Gly  Asp Ser Leu His Glu  His Ile Ala Asn Leu  Ala Gly Ser 
    1475                 1480                 1485             


Pro Ala  Ile Lys Lys Gly Ile  Leu Gln Thr Val Lys  Val Val Asp 
    1490                 1495                 1500             


Glu Leu  Val Lys Val Met Gly  Arg His Lys Pro Glu  Asn Ile Val 
    1505                 1510                 1515             


Ile Glu  Met Ala Arg Glu Asn  Gln Thr Thr Gln Lys  Gly Gln Lys 
    1520                 1525                 1530             


Asn Ser  Arg Glu Arg Met Lys  Arg Ile Glu Glu Gly  Ile Lys Glu 
    1535                 1540                 1545             


Leu Gly  Ser Gln Ile Leu Lys  Glu His Pro Val Glu  Asn Thr Gln 
    1550                 1555                 1560             


Leu Gln  Asn Glu Lys Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg 
    1565                 1570                 1575             


Asp Met  Tyr Val Asp Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp 
    1580                 1585                 1590             


Tyr Asp  Val Asp His Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp 
    1595                 1600                 1605             


Ser Ile  Asp Asn Lys Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly 
    1610                 1615                 1620             


Lys Ser  Asp Asn Val Pro Ser  Glu Glu Val Val Lys  Lys Met Lys 
    1625                 1630                 1635             


Asn Tyr  Trp Arg Gln Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg 
    1640                 1645                 1650             


Lys Phe  Asp Asn Leu Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu 
    1655                 1660                 1665             


Leu Asp  Lys Ala Gly Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg 
    1670                 1675                 1680             


Gln Ile  Thr Lys His Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn 
    1685                 1690                 1695             


Thr Lys  Tyr Asp Glu Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val 
    1700                 1705                 1710             


Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe 
    1715                 1720                 1725             


Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn Tyr His  His Ala His 
    1730                 1735                 1740             


Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys 
    1745                 1750                 1755             


Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val 
    1760                 1765                 1770             


Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser Glu Gln  Glu Gly Ala 
    1775                 1780                 1785             


Asp Lys  Arg Thr Ala Asp Gly  Ser Glu Phe Glu Ser  Pro Lys Lys 
    1790                 1795                 1800             


Lys Arg  Lys Val 
    1805         


<210>  348
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.8-m polypeptide sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(1566)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1567)..(1588)
<223>  Bipartite nuclear localization sequence

<400>  348

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1520                 1525                 1530             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  349
<211>  1786
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.8-d polypeptide sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(365)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (366)..(397)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (398)..(1764)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1765)..(1786)
<223>  Bipartite nuclear localization sequence

<400>  349

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr 
        195                 200                 205             


Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg 
    210                 215                 220                 


Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly 
225                 230                 235                 240 


Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala 
                245                 250                 255     


Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg 
            260                 265                 270         


Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys 
        275                 280                 285             


Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val 
    290                 295                 300                 


Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His 
305                 310                 315                 320 


His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala 
                325                 330                 335     


Asp Glu Cys Ala Ala Leu Leu Cys Arg Phe Phe Arg Met Pro Arg Arg 
            340                 345                 350         


Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly 
        355                 360                 365             


Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
    370                 375                 380                 


Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys 
385                 390                 395                 400 


Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val 
                405                 410                 415     


Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly 
            420                 425                 430         


Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu 
        435                 440                 445             


Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala 
    450                 455                 460                 


Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu 
465                 470                 475                 480 


Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg 
                485                 490                 495     


Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His 
            500                 505                 510         


Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr 
        515                 520                 525             


Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys 
    530                 535                 540                 


Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe 
545                 550                 555                 560 


Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp 
                565                 570                 575     


Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe 
            580                 585                 590         


Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu 
        595                 600                 605             


Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln 
    610                 615                 620                 


Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu 
625                 630                 635                 640 


Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu 
                645                 650                 655     


Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp 
            660                 665                 670         


Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala 
        675                 680                 685             


Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val 
    690                 695                 700                 


Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg 
705                 710                 715                 720 


Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg 
                725                 730                 735     


Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys 
            740                 745                 750         


Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe 
        755                 760                 765             


Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu 
    770                 775                 780                 


Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr 
785                 790                 795                 800 


Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His 
                805                 810                 815     


Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn 
            820                 825                 830         


Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val 
        835                 840                 845             


Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys 
    850                 855                 860                 


Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys 
865                 870                 875                 880 


Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys 
                885                 890                 895     


Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu 
            900                 905                 910         


Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu 
        915                 920                 925             


Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile 
    930                 935                 940                 


Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu 
945                 950                 955                 960 


Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile 
                965                 970                 975     


Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp 
            980                 985                 990         


Leu Leu Lys Ile Ile Lys Asp Lys  Asp Phe Leu Asp Asn  Glu Glu Asn 
        995                 1000                 1005             


Glu Asp  Ile Leu Glu Asp Ile  Val Leu Thr Leu Thr  Leu Phe Glu 
    1010                 1015                 1020             


Asp Arg  Glu Met Ile Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu 
    1025                 1030                 1035             


Phe Asp  Asp Lys Val Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr 
    1040                 1045                 1050             


Gly Trp  Gly Arg Leu Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp 
    1055                 1060                 1065             


Lys Gln  Ser Gly Lys Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly 
    1070                 1075                 1080             


Phe Ala  Asn Arg Asn Phe Met  Gln Leu Ile His Asp  Asp Ser Leu 
    1085                 1090                 1095             


Thr Phe  Lys Glu Asp Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly 
    1100                 1105                 1110             


Asp Ser  Leu His Glu His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala 
    1115                 1120                 1125             


Ile Lys  Lys Gly Ile Leu Gln  Thr Val Lys Val Val  Asp Glu Leu 
    1130                 1135                 1140             


Val Lys  Val Met Gly Arg His  Lys Pro Glu Asn Ile  Val Ile Glu 
    1145                 1150                 1155             


Met Ala  Arg Glu Asn Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser 
    1160                 1165                 1170             


Arg Glu  Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
    1175                 1180                 1185             


Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln 
    1190                 1195                 1200             


Asn Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met 
    1205                 1210                 1215             


Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp 
    1220                 1225                 1230             


Val Asp  His Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile 
    1235                 1240                 1245             


Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser 
    1250                 1255                 1260             


Asp Asn  Val Pro Ser Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr 
    1265                 1270                 1275             


Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe 
    1280                 1285                 1290             


Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp 
    1295                 1300                 1305             


Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile 
    1310                 1315                 1320             


Thr Lys  His Val Ala Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys 
    1325                 1330                 1335             


Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr 
    1340                 1345                 1350             


Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe 
    1355                 1360                 1365             


Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  His Asp Ala 
    1370                 1375                 1380             


Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro 
    1385                 1390                 1395             


Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1400                 1405                 1410             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1415                 1420                 1425             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1430                 1435                 1440             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1445                 1450                 1455             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1460                 1465                 1470             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1475                 1480                 1485             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1490                 1495                 1500             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1505                 1510                 1515             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1520                 1525                 1530             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1535                 1540                 1545             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1550                 1555                 1560             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1565                 1570                 1575             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1580                 1585                 1590             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1595                 1600                 1605             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1610                 1615                 1620             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1625                 1630                 1635             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1640                 1645                 1650             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1655                 1660                 1665             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1670                 1675                 1680             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1685                 1690                 1695             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1700                 1705                 1710             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1715                 1720                 1725             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1730                 1735                 1740             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1745                 1750                 1755             


Ser Gln  Leu Gly Gly Asp Glu  Gly Ala Asp Lys Arg  Thr Ala Asp 
    1760                 1765                 1770             


Gly Ser  Glu Phe Glu Ser Pro  Lys Lys Lys Arg Lys  Val 
    1775                 1780                 1785     


<210>  350
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.13-m polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(1566)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1567)..(1588)
<223>  Bipartite nuclear localization sequence

<400>  350

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1520                 1525                 1530             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  351
<211>  1786
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.13-d polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(365)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (366)..(397)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (398)..(1764)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1765)..(1786)
<223>  Bipartite nuclear localization sequence

<400>  351

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr 
        195                 200                 205             


Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg 
    210                 215                 220                 


Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly 
225                 230                 235                 240 


Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala 
                245                 250                 255     


Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg 
            260                 265                 270         


Leu Tyr Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys 
        275                 280                 285             


Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val 
    290                 295                 300                 


Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His 
305                 310                 315                 320 


His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala 
                325                 330                 335     


Asp Glu Cys Ala Ala Leu Leu Cys Arg Phe Phe Arg Met Pro Arg Arg 
            340                 345                 350         


Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly 
        355                 360                 365             


Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
    370                 375                 380                 


Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys 
385                 390                 395                 400 


Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val 
                405                 410                 415     


Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly 
            420                 425                 430         


Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu 
        435                 440                 445             


Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala 
    450                 455                 460                 


Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu 
465                 470                 475                 480 


Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg 
                485                 490                 495     


Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His 
            500                 505                 510         


Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr 
        515                 520                 525             


Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys 
    530                 535                 540                 


Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe 
545                 550                 555                 560 


Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp 
                565                 570                 575     


Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe 
            580                 585                 590         


Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu 
        595                 600                 605             


Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln 
    610                 615                 620                 


Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu 
625                 630                 635                 640 


Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu 
                645                 650                 655     


Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp 
            660                 665                 670         


Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala 
        675                 680                 685             


Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val 
    690                 695                 700                 


Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg 
705                 710                 715                 720 


Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg 
                725                 730                 735     


Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys 
            740                 745                 750         


Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe 
        755                 760                 765             


Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu 
    770                 775                 780                 


Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr 
785                 790                 795                 800 


Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His 
                805                 810                 815     


Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn 
            820                 825                 830         


Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val 
        835                 840                 845             


Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys 
    850                 855                 860                 


Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys 
865                 870                 875                 880 


Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys 
                885                 890                 895     


Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu 
            900                 905                 910         


Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu 
        915                 920                 925             


Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile 
    930                 935                 940                 


Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu 
945                 950                 955                 960 


Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile 
                965                 970                 975     


Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp 
            980                 985                 990         


Leu Leu Lys Ile Ile Lys Asp Lys  Asp Phe Leu Asp Asn  Glu Glu Asn 
        995                 1000                 1005             


Glu Asp  Ile Leu Glu Asp Ile  Val Leu Thr Leu Thr  Leu Phe Glu 
    1010                 1015                 1020             


Asp Arg  Glu Met Ile Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu 
    1025                 1030                 1035             


Phe Asp  Asp Lys Val Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr 
    1040                 1045                 1050             


Gly Trp  Gly Arg Leu Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp 
    1055                 1060                 1065             


Lys Gln  Ser Gly Lys Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly 
    1070                 1075                 1080             


Phe Ala  Asn Arg Asn Phe Met  Gln Leu Ile His Asp  Asp Ser Leu 
    1085                 1090                 1095             


Thr Phe  Lys Glu Asp Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly 
    1100                 1105                 1110             


Asp Ser  Leu His Glu His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala 
    1115                 1120                 1125             


Ile Lys  Lys Gly Ile Leu Gln  Thr Val Lys Val Val  Asp Glu Leu 
    1130                 1135                 1140             


Val Lys  Val Met Gly Arg His  Lys Pro Glu Asn Ile  Val Ile Glu 
    1145                 1150                 1155             


Met Ala  Arg Glu Asn Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser 
    1160                 1165                 1170             


Arg Glu  Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
    1175                 1180                 1185             


Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln 
    1190                 1195                 1200             


Asn Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met 
    1205                 1210                 1215             


Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp 
    1220                 1225                 1230             


Val Asp  His Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile 
    1235                 1240                 1245             


Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser 
    1250                 1255                 1260             


Asp Asn  Val Pro Ser Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr 
    1265                 1270                 1275             


Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe 
    1280                 1285                 1290             


Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp 
    1295                 1300                 1305             


Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile 
    1310                 1315                 1320             


Thr Lys  His Val Ala Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys 
    1325                 1330                 1335             


Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr 
    1340                 1345                 1350             


Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe 
    1355                 1360                 1365             


Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  His Asp Ala 
    1370                 1375                 1380             


Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro 
    1385                 1390                 1395             


Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1400                 1405                 1410             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1415                 1420                 1425             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1430                 1435                 1440             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1445                 1450                 1455             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1460                 1465                 1470             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1475                 1480                 1485             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1490                 1495                 1500             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1505                 1510                 1515             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1520                 1525                 1530             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1535                 1540                 1545             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1550                 1555                 1560             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1565                 1570                 1575             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1580                 1585                 1590             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1595                 1600                 1605             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1610                 1615                 1620             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1625                 1630                 1635             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1640                 1645                 1650             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1655                 1660                 1665             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1670                 1675                 1680             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1685                 1690                 1695             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1700                 1705                 1710             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1715                 1720                 1725             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1730                 1735                 1740             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1745                 1750                 1755             


Ser Gln  Leu Gly Gly Asp Glu  Gly Ala Asp Lys Arg  Thr Ala Asp 
    1760                 1765                 1770             


Gly Ser  Glu Phe Glu Ser Pro  Lys Lys Lys Arg Lys  Val 
    1775                 1780                 1785     


<210>  352
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.17-m polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(1566)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1567)..(1588)
<223>  Bipartite nuclear localization sequence

<400>  352

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Ser Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1520                 1525                 1530             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  353
<211>  1786
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.17-d polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(365)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (366)..(397)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (398)..(1764)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1765)..(1786)
<223>  Bipartite nuclear localization sequence

<400>  353

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr 
        195                 200                 205             


Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg 
    210                 215                 220                 


Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly 
225                 230                 235                 240 


Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala 
                245                 250                 255     


Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg 
            260                 265                 270         


Leu Ile Asp Ala Thr Leu Tyr Ser Thr Phe Glu Pro Cys Val Met Cys 
        275                 280                 285             


Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val 
    290                 295                 300                 


Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His 
305                 310                 315                 320 


Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala 
                325                 330                 335     


Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg Arg 
            340                 345                 350         


Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly 
        355                 360                 365             


Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
    370                 375                 380                 


Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys 
385                 390                 395                 400 


Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val 
                405                 410                 415     


Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly 
            420                 425                 430         


Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu 
        435                 440                 445             


Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala 
    450                 455                 460                 


Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu 
465                 470                 475                 480 


Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg 
                485                 490                 495     


Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His 
            500                 505                 510         


Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr 
        515                 520                 525             


Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys 
    530                 535                 540                 


Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe 
545                 550                 555                 560 


Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp 
                565                 570                 575     


Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe 
            580                 585                 590         


Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu 
        595                 600                 605             


Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln 
    610                 615                 620                 


Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu 
625                 630                 635                 640 


Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu 
                645                 650                 655     


Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp 
            660                 665                 670         


Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala 
        675                 680                 685             


Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val 
    690                 695                 700                 


Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg 
705                 710                 715                 720 


Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg 
                725                 730                 735     


Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys 
            740                 745                 750         


Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe 
        755                 760                 765             


Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu 
    770                 775                 780                 


Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr 
785                 790                 795                 800 


Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His 
                805                 810                 815     


Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn 
            820                 825                 830         


Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val 
        835                 840                 845             


Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys 
    850                 855                 860                 


Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys 
865                 870                 875                 880 


Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys 
                885                 890                 895     


Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu 
            900                 905                 910         


Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu 
        915                 920                 925             


Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile 
    930                 935                 940                 


Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu 
945                 950                 955                 960 


Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile 
                965                 970                 975     


Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp 
            980                 985                 990         


Leu Leu Lys Ile Ile Lys Asp Lys  Asp Phe Leu Asp Asn  Glu Glu Asn 
        995                 1000                 1005             


Glu Asp  Ile Leu Glu Asp Ile  Val Leu Thr Leu Thr  Leu Phe Glu 
    1010                 1015                 1020             


Asp Arg  Glu Met Ile Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu 
    1025                 1030                 1035             


Phe Asp  Asp Lys Val Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr 
    1040                 1045                 1050             


Gly Trp  Gly Arg Leu Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp 
    1055                 1060                 1065             


Lys Gln  Ser Gly Lys Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly 
    1070                 1075                 1080             


Phe Ala  Asn Arg Asn Phe Met  Gln Leu Ile His Asp  Asp Ser Leu 
    1085                 1090                 1095             


Thr Phe  Lys Glu Asp Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly 
    1100                 1105                 1110             


Asp Ser  Leu His Glu His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala 
    1115                 1120                 1125             


Ile Lys  Lys Gly Ile Leu Gln  Thr Val Lys Val Val  Asp Glu Leu 
    1130                 1135                 1140             


Val Lys  Val Met Gly Arg His  Lys Pro Glu Asn Ile  Val Ile Glu 
    1145                 1150                 1155             


Met Ala  Arg Glu Asn Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser 
    1160                 1165                 1170             


Arg Glu  Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
    1175                 1180                 1185             


Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln 
    1190                 1195                 1200             


Asn Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met 
    1205                 1210                 1215             


Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp 
    1220                 1225                 1230             


Val Asp  His Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile 
    1235                 1240                 1245             


Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser 
    1250                 1255                 1260             


Asp Asn  Val Pro Ser Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr 
    1265                 1270                 1275             


Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe 
    1280                 1285                 1290             


Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp 
    1295                 1300                 1305             


Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile 
    1310                 1315                 1320             


Thr Lys  His Val Ala Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys 
    1325                 1330                 1335             


Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr 
    1340                 1345                 1350             


Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe 
    1355                 1360                 1365             


Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  His Asp Ala 
    1370                 1375                 1380             


Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro 
    1385                 1390                 1395             


Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1400                 1405                 1410             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1415                 1420                 1425             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1430                 1435                 1440             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1445                 1450                 1455             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1460                 1465                 1470             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1475                 1480                 1485             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1490                 1495                 1500             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1505                 1510                 1515             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1520                 1525                 1530             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1535                 1540                 1545             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1550                 1555                 1560             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1565                 1570                 1575             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1580                 1585                 1590             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1595                 1600                 1605             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1610                 1615                 1620             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1625                 1630                 1635             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1640                 1645                 1650             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1655                 1660                 1665             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1670                 1675                 1680             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1685                 1690                 1695             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1700                 1705                 1710             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1715                 1720                 1725             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1730                 1735                 1740             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1745                 1750                 1755             


Ser Gln  Leu Gly Gly Asp Glu  Gly Ala Asp Lys Arg  Thr Ala Asp 
    1760                 1765                 1770             


Gly Ser  Glu Phe Glu Ser Pro  Lys Lys Lys Arg Lys  Val 
    1775                 1780                 1785     


<210>  354
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.20-m polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminse sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(1566)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1567)..(1588)
<223>  Bipartite nuclear localization sequence

<400>  354

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Ser Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Gly Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg 
    1520                 1525                 1530             


Tyr Thr  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  355
<211>  1786
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: ABE8.20-d polypeptide 
       sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(167)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (168)..(199)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (200)..(365)
<223>  Adenosine deaminase sequence

<220>
<221>  MISC_FEATURE
<222>  (366)..(397)
<223>  Linker sequence

<220>
<221>  MISC_FEATURE
<222>  (398)..(1764)
<223>  Sequence derived from Cas9

<220>
<221>  MISC_FEATURE
<222>  (1765)..(1786)
<223>  Bipartite nuclear localization sequence

<400>  355

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr 
        195                 200                 205             


Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg 
    210                 215                 220                 


Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly 
225                 230                 235                 240 


Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala 
                245                 250                 255     


Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg 
            260                 265                 270         


Leu Tyr Asp Ala Thr Leu Tyr Ser Thr Phe Glu Pro Cys Val Met Cys 
        275                 280                 285             


Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val 
    290                 295                 300                 


Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His 
305                 310                 315                 320 


His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala 
                325                 330                 335     


Asp Glu Cys Ala Ala Leu Leu Cys Arg Phe Phe Arg Met Pro Arg Arg 
            340                 345                 350         


Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly 
        355                 360                 365             


Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
    370                 375                 380                 


Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys 
385                 390                 395                 400 


Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val 
                405                 410                 415     


Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly 
            420                 425                 430         


Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu 
        435                 440                 445             


Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala 
    450                 455                 460                 


Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu 
465                 470                 475                 480 


Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg 
                485                 490                 495     


Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His 
            500                 505                 510         


Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr 
        515                 520                 525             


Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys 
    530                 535                 540                 


Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe 
545                 550                 555                 560 


Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp 
                565                 570                 575     


Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe 
            580                 585                 590         


Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu 
        595                 600                 605             


Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln 
    610                 615                 620                 


Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu 
625                 630                 635                 640 


Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu 
                645                 650                 655     


Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp 
            660                 665                 670         


Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala 
        675                 680                 685             


Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val 
    690                 695                 700                 


Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg 
705                 710                 715                 720 


Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg 
                725                 730                 735     


Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys 
            740                 745                 750         


Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe 
        755                 760                 765             


Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu 
    770                 775                 780                 


Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr 
785                 790                 795                 800 


Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His 
                805                 810                 815     


Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn 
            820                 825                 830         


Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val 
        835                 840                 845             


Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys 
    850                 855                 860                 


Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys 
865                 870                 875                 880 


Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys 
                885                 890                 895     


Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu 
            900                 905                 910         


Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu 
        915                 920                 925             


Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile 
    930                 935                 940                 


Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu 
945                 950                 955                 960 


Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile 
                965                 970                 975     


Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp 
            980                 985                 990         


Leu Leu Lys Ile Ile Lys Asp Lys  Asp Phe Leu Asp Asn  Glu Glu Asn 
        995                 1000                 1005             


Glu Asp  Ile Leu Glu Asp Ile  Val Leu Thr Leu Thr  Leu Phe Glu 
    1010                 1015                 1020             


Asp Arg  Glu Met Ile Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu 
    1025                 1030                 1035             


Phe Asp  Asp Lys Val Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr 
    1040                 1045                 1050             


Gly Trp  Gly Arg Leu Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp 
    1055                 1060                 1065             


Lys Gln  Ser Gly Lys Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly 
    1070                 1075                 1080             


Phe Ala  Asn Arg Asn Phe Met  Gln Leu Ile His Asp  Asp Ser Leu 
    1085                 1090                 1095             


Thr Phe  Lys Glu Asp Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly 
    1100                 1105                 1110             


Asp Ser  Leu His Glu His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala 
    1115                 1120                 1125             


Ile Lys  Lys Gly Ile Leu Gln  Thr Val Lys Val Val  Asp Glu Leu 
    1130                 1135                 1140             


Val Lys  Val Met Gly Arg His  Lys Pro Glu Asn Ile  Val Ile Glu 
    1145                 1150                 1155             


Met Ala  Arg Glu Asn Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser 
    1160                 1165                 1170             


Arg Glu  Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
    1175                 1180                 1185             


Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln 
    1190                 1195                 1200             


Asn Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met 
    1205                 1210                 1215             


Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp 
    1220                 1225                 1230             


Val Asp  His Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile 
    1235                 1240                 1245             


Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser 
    1250                 1255                 1260             


Asp Asn  Val Pro Ser Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr 
    1265                 1270                 1275             


Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe 
    1280                 1285                 1290             


Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp 
    1295                 1300                 1305             


Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile 
    1310                 1315                 1320             


Thr Lys  His Val Ala Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys 
    1325                 1330                 1335             


Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr 
    1340                 1345                 1350             


Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe 
    1355                 1360                 1365             


Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  His Asp Ala 
    1370                 1375                 1380             


Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro 
    1385                 1390                 1395             


Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp 
    1400                 1405                 1410             


Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
    1415                 1420                 1425             


Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys 
    1430                 1435                 1440             


Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu 
    1445                 1450                 1455             


Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly 
    1460                 1465                 1470             


Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val 
    1475                 1480                 1485             


Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys 
    1490                 1495                 1500             


Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg 
    1505                 1510                 1515             


Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro 
    1520                 1525                 1530             


Thr Val  Ala Tyr Ser Val Leu  Val Val Ala Lys Val  Glu Lys Gly 
    1535                 1540                 1545             


Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr 
    1550                 1555                 1560             


Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu 
    1565                 1570                 1575             


Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys 
    1580                 1585                 1590             


Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg 
    1595                 1600                 1605             


Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala 
    1610                 1615                 1620             


Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr 
    1625                 1630                 1635             


Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu 
    1640                 1645                 1650             


Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
    1655                 1660                 1665             


Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu 
    1670                 1675                 1680             


Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile 
    1685                 1690                 1695             


Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn 
    1700                 1705                 1710             


Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp 
    1715                 1720                 1725             


Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu 
    1730                 1735                 1740             


Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu 
    1745                 1750                 1755             


Ser Gln  Leu Gly Gly Asp Glu  Gly Ala Asp Lys Arg  Thr Ala Asp 
    1760                 1765                 1770             


Gly Ser  Glu Phe Glu Ser Pro  Lys Lys Lys Arg Lys  Val 
    1775                 1780                 1785     


<210>  356
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 01. monoABE8.1_bpNLS + Y147T 
       polypeptide sequence

<400>  356

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Thr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  357
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 02. monoABE8.1_bpNLS + Y147R 
       polypeptide sequence

<400>  357

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  358
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 03. monoABE8.1_bpNLS + Q154S 
       polypeptide sequence

<400>  358

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Ser Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  359
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 04. monoABE8.1_bpNLS + Y123H 
       polypeptide sequence

<400>  359

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  360
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 05. monoABE8.1_bpNLS + V82S 
       polypeptide sequence

<400>  360

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Ser Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  361
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 06. monoABE8.1_bpNLS + T166R 
       polypeptide sequence

<400>  361

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Arg Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  362
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 07. monoABE8.1_bpNLS + Q154R 
       polypeptide sequence

<400>  362

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  363
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 08. monoABE8.1_bpNLS + 
       Y147R_Q154R_Y123H polypeptide sequence

<400>  363

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  364
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 09. monoABE8.1_bpNLS + 
       Y147R_Q154R_I76Y polypeptide sequence

<400>  364

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  365
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 10. monoABE8.1_bpNLS + 
       Y147R_Q154R_T166R polypeptide sequence

<400>  365

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Arg Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  366
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 11. monoABE8.1_bpNLS + 
       Y147T_Q154R polypeptide sequence

<400>  366

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Thr Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  367
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 12. monoABE8.1_bpNLS + 
       Y147T_Q154S polypeptide sequence

<400>  367

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Thr Phe Phe Arg Met Pro Arg Ser Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  368
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 13. monoABE8.1_bpNLS + 
       H123Y123H_Y147R_Q154R_I76Y polypeptide sequence

<400>  368

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Tyr Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Arg Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  369
<211>  1588
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: 14. monoABE8.1_bpNLS + V82S +
       Q154R polypeptide sequence

<400>  369

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala 
        35                  40                  45              


Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Ser Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Cys Tyr Phe Phe Arg Met Pro Arg Arg Val Phe Asn Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser 
            180                 185                 190         


Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
        195                 200                 205             


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
    210                 215                 220                 


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
225                 230                 235                 240 


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
                245                 250                 255     


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
            260                 265                 270         


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
        275                 280                 285             


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
    290                 295                 300                 


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
305                 310                 315                 320 


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
                325                 330                 335     


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
            340                 345                 350         


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
        355                 360                 365             


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
    370                 375                 380                 


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
385                 390                 395                 400 


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
                405                 410                 415     


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
            420                 425                 430         


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
        435                 440                 445             


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
    450                 455                 460                 


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
465                 470                 475                 480 


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
                485                 490                 495     


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
            500                 505                 510         


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
        515                 520                 525             


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
    530                 535                 540                 


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
545                 550                 555                 560 


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
                565                 570                 575     


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
            580                 585                 590         


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
        595                 600                 605             


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
    610                 615                 620                 


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
625                 630                 635                 640 


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
                645                 650                 655     


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
            660                 665                 670         


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
        675                 680                 685             


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
    690                 695                 700                 


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
705                 710                 715                 720 


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
                725                 730                 735     


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
            740                 745                 750         


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
        755                 760                 765             


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
    770                 775                 780                 


Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys 
785                 790                 795                 800 


Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp 
                805                 810                 815     


Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu 
            820                 825                 830         


Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln 
        835                 840                 845             


Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu 
    850                 855                 860                 


Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe 
865                 870                 875                 880 


Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His 
                885                 890                 895     


Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser 
            900                 905                 910         


Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser 
        915                 920                 925             


Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu 
    930                 935                 940                 


Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu 
945                 950                 955                 960 


Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg 
                965                 970                 975     


Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln 
            980                 985                 990         


Ile Leu Lys Glu His Pro Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys 
        995                 1000                 1005             


Leu Tyr  Leu Tyr Tyr Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp 
    1010                 1015                 1020             


Gln Glu  Leu Asp Ile Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His 
    1025                 1030                 1035             


Ile Val  Pro Gln Ser Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys 
    1040                 1045                 1050             


Val Leu  Thr Arg Ser Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val 
    1055                 1060                 1065             


Pro Ser  Glu Glu Val Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln 
    1070                 1075                 1080             


Leu Leu  Asn Ala Lys Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu 
    1085                 1090                 1095             


Thr Lys  Ala Glu Arg Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly 
    1100                 1105                 1110             


Phe Ile  Lys Arg Gln Leu Val  Glu Thr Arg Gln Ile  Thr Lys His 
    1115                 1120                 1125             


Val Ala  Gln Ile Leu Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu 
    1130                 1135                 1140             


Asn Asp  Lys Leu Ile Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser 
    1145                 1150                 1155             


Lys Leu  Val Ser Asp Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val 
    1160                 1165                 1170             


Arg Glu  Ile Asn Asn Tyr His  His Ala His Asp Ala  Tyr Leu Asn 
    1175                 1180                 1185             


Ala Val  Val Gly Thr Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu 
    1190                 1195                 1200             


Ser Glu  Phe Val Tyr Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys 
    1205                 1210                 1215             


Met Ile  Ala Lys Ser Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys 
    1220                 1225                 1230             


Tyr Phe  Phe Tyr Ser Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile 
    1235                 1240                 1245             


Thr Leu  Ala Asn Gly Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr 
    1250                 1255                 1260             


Asn Gly  Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe 
    1265                 1270                 1275             


Ala Thr  Val Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val 
    1280                 1285                 1290             


Lys Lys  Thr Glu Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile 
    1295                 1300                 1305             


Leu Pro  Lys Arg Asn Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp 
    1310                 1315                 1320             


Trp Asp  Pro Lys Lys Tyr Gly  Gly Phe Val Ser Pro  Thr Val Ala 
    1325                 1330                 1335             


Tyr Ser  Val Leu Val Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys 
    1340                 1345                 1350             


Lys Leu  Lys Ser Val Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu 
    1355                 1360                 1365             


Arg Ser  Ser Phe Glu Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys 
    1370                 1375                 1380             


Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys 
    1385                 1390                 1395             


Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala 
    1400                 1405                 1410             


Ser Ala  Arg Glu Leu Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser 
    1415                 1420                 1425             


Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu 
    1430                 1435                 1440             


Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu 
    1445                 1450                 1455             


Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
    1460                 1465                 1470             


Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val 
    1475                 1480                 1485             


Leu Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln 
    1490                 1495                 1500             


Ala Glu  Asn Ile Ile His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala 
    1505                 1510                 1515             


Pro Ala  Ala Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Gln 
    1520                 1525                 1530             


Tyr Arg  Ser Thr Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln 
    1535                 1540                 1545             


Ser Ile  Thr Gly Leu Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu 
    1550                 1555                 1560             


Gly Gly  Asp Glu Gly Ala Asp  Lys Arg Thr Ala Asp  Gly Ser Glu 
    1565                 1570                 1575             


Phe Glu  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210>  370
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  370

Ser Gly Gly Ser 
1               


<210>  371
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  371

Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala 
1               5                   10                  15      


Thr Pro Glu Ser Ser Gly Gly Ser 
            20                  


<210>  372
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  372

Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
            20                  25                  30          


<210>  373
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  373

Gly Gly Ser Gly Gly Ser Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser 
1               5                   10                  15      


Thr Glu Glu Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly 
            20                  25                  30          


Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Ser Pro Ala Gly 
        35                  40                  45              


Ser Pro Thr Ser Thr Glu Glu Gly Thr Ser Thr Glu Pro Ser Glu Gly 
    50                  55                  60                  


Ser Ala Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
65                  70                  75                  80  


Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala 
                85                  90                  95      


Thr Ser Gly Gly Ser Gly Gly Ser 
            100                 


<210>  374
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  374

Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      


Ser Glu Ser Ala Thr Pro Glu Ser 
            20                  


<210>  375
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  375

Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
            20                  25                  30          


Ser Gly Gly Ser Ser Gly Gly Ser 
        35                  40  


<210>  376
<211>  64
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  376

Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
            20                  25                  30          


Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
        35                  40                  45              


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
    50                  55                  60                  


<210>  377
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polypeptide 
       sequence

<400>  377

Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly Thr Ser 
1               5                   10                  15      


Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Thr Ser Thr Glu Pro Ser 
            20                  25                  30          


Glu Gly Ser Ala Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu 
        35                  40                  45              


Glu Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser 
    50                  55                  60                  


Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Glu Ser Ala Thr 
65                  70                  75                  80  


Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser 
                85                  90          


<210>  378
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  378

Pro Ala Pro Ala Pro 
1               5   


<210>  379
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  379

Pro Ala Pro Ala Pro Ala 
1               5       


<210>  380
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  380

Pro Ala Pro Ala Pro Ala Pro 
1               5           


<210>  381
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  381

Pro Ala Pro Ala Pro Ala Pro Ala 
1               5               


<210>  382
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  382

Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   


<210>  383
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  383

Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   10                  15  


<210>  384
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic peptide sequence

<400>  384

Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala 
1               5                   10                  15      


Pro Ala Pro Ala Pro 
            20      


<210>  385
<211>  4
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  TXXH motif peptide sequence

<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa is any amino acid

<400>  385

Thr Xaa Xaa His 
1               


<210>  386
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  386
gcacccaggg gttctgcaga gcagg                                             25


<210>  387
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  387
gcattccact ccgtccgcct c                                                 21


<210>  388
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  388
gctgcaaggg ttggccaggc t                                                 21


<210>  389
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence

<400>  389
ggagccagag accagtgggc a                                                 21


<210>  390
<211>  1081
<212>  PRT
<213>  Neisseria meningitidis

<400>  390

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu 
            20                  25                  30          


Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Cys Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Asn Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Ser Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Thr Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Ala Tyr Ala Asp Glu Glu Asp Trp Thr Val Ile Asp 
            980                 985                 990         


Glu Ser Phe Arg Phe Lys Phe Val  Leu Tyr Ser Asn Asp  Leu Ile Lys 
        995                 1000                 1005             


Val Gln  Leu Lys Lys Asp Ser  Phe Leu Gly Tyr Phe  Ser Gly Leu 
    1010                 1015                 1020             


Asp Arg  Ala Thr Gly Ala Ile  Ser Leu Arg Glu His  Asp Leu Glu 
    1025                 1030                 1035             


Lys Ser  Lys Gly Lys Asp Gly  Met His Arg Ile Gly  Val Lys Thr 
    1040                 1045                 1050             


Ala Leu  Ser Phe Gln Lys Tyr  Gln Ile Asp Glu Met  Gly Lys Glu 
    1055                 1060                 1065             


Ile Arg  Pro Cys Arg Leu Lys  Lys Arg Pro Pro Val  Arg 
    1070                 1075                 1080     


<210>  391
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Telomerase Ku Binding Motif 
       nucleotide sequence

<400>  391
uucuugucgu acuuauagau cgcuacguua uuucaauuuu gaaaaucuga guccugggag       60

ugcgga                                                                  66


<210>  392
<211>  1094
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Telomerase Ku Heterodimer 
       polypeptide sequence

<400>  392

Met Ser Gly Trp Glu Ser Tyr Tyr Lys Thr Glu Gly Asp Glu Glu Ala 
1               5                   10                  15      


Glu Glu Glu Gln Glu Glu Asn Leu Glu Ala Ser Gly Asp Tyr Lys Tyr 
            20                  25                  30          


Ser Gly Arg Asp Ser Leu Ile Phe Leu Val Asp Ala Ser Lys Ala Met 
        35                  40                  45              


Phe Glu Ser Gln Ser Glu Asp Glu Leu Thr Pro Phe Asp Met Ser Ile 
    50                  55                  60                  


Gln Cys Ile Gln Ser Val Tyr Ile Ser Lys Ile Ile Ser Ser Asp Arg 
65                  70                  75                  80  


Asp Leu Leu Ala Val Val Phe Tyr Gly Thr Glu Lys Asp Lys Asn Ser 
                85                  90                  95      


Val Asn Phe Lys Asn Ile Tyr Val Leu Gln Glu Leu Asp Asn Pro Gly 
            100                 105                 110         


Ala Lys Arg Ile Leu Glu Leu Asp Gln Phe Lys Gly Gln Gln Gly Gln 
        115                 120                 125             


Lys Arg Phe Gln Asp Met Met Gly His Gly Ser Asp Tyr Ser Leu Ser 
    130                 135                 140                 


Glu Val Leu Trp Val Cys Ala Asn Leu Phe Ser Asp Val Gln Phe Lys 
145                 150                 155                 160 


Met Ser His Lys Arg Ile Met Leu Phe Thr Asn Glu Asp Asn Pro His 
                165                 170                 175     


Gly Asn Asp Ser Ala Lys Ala Ser Arg Ala Arg Thr Lys Ala Gly Asp 
            180                 185                 190         


Leu Arg Asp Thr Gly Ile Phe Leu Asp Leu Met His Leu Lys Lys Pro 
        195                 200                 205             


Gly Gly Phe Asp Ile Ser Leu Phe Tyr Arg Asp Ile Ile Ser Ile Ala 
    210                 215                 220                 


Glu Asp Glu Asp Leu Arg Val His Phe Glu Glu Ser Ser Lys Leu Glu 
225                 230                 235                 240 


Asp Leu Leu Arg Lys Val Arg Ala Lys Glu Thr Arg Lys Arg Ala Leu 
                245                 250                 255     


Ser Arg Leu Lys Leu Lys Leu Asn Lys Asp Ile Val Ile Ser Val Gly 
            260                 265                 270         


Ile Tyr Asn Leu Val Gln Lys Ala Leu Lys Pro Pro Pro Ile Lys Leu 
        275                 280                 285             


Tyr Arg Glu Thr Asn Glu Pro Val Lys Thr Lys Thr Arg Thr Phe Asn 
    290                 295                 300                 


Thr Ser Thr Gly Gly Leu Leu Leu Pro Ser Asp Thr Lys Arg Ser Gln 
305                 310                 315                 320 


Ile Tyr Gly Ser Arg Gln Ile Ile Leu Glu Lys Glu Glu Thr Glu Glu 
                325                 330                 335     


Leu Lys Arg Phe Asp Asp Pro Gly Leu Met Leu Met Gly Phe Lys Pro 
            340                 345                 350         


Leu Val Leu Leu Lys Lys His His Tyr Leu Arg Pro Ser Leu Phe Val 
        355                 360                 365             


Tyr Pro Glu Glu Ser Leu Val Ile Gly Ser Ser Thr Leu Phe Ser Ala 
    370                 375                 380                 


Leu Leu Ile Lys Cys Leu Glu Lys Glu Val Ala Ala Leu Cys Arg Tyr 
385                 390                 395                 400 


Thr Pro Arg Arg Asn Ile Pro Pro Tyr Phe Val Ala Leu Val Pro Gln 
                405                 410                 415     


Glu Glu Glu Leu Asp Asp Gln Lys Ile Gln Val Thr Pro Pro Gly Phe 
            420                 425                 430         


Gln Leu Val Phe Leu Pro Phe Ala Asp Asp Lys Arg Lys Met Pro Phe 
        435                 440                 445             


Thr Glu Lys Ile Met Ala Thr Pro Glu Gln Val Gly Lys Met Lys Ala 
    450                 455                 460                 


Ile Val Glu Lys Leu Arg Phe Thr Tyr Arg Ser Asp Ser Phe Glu Asn 
465                 470                 475                 480 


Pro Val Leu Gln Gln His Phe Arg Asn Leu Glu Ala Leu Ala Leu Asp 
                485                 490                 495     


Leu Met Glu Pro Glu Gln Ala Val Asp Leu Thr Leu Pro Lys Val Glu 
            500                 505                 510         


Ala Met Asn Lys Arg Leu Gly Ser Leu Val Asp Glu Phe Lys Glu Leu 
        515                 520                 525             


Val Tyr Pro Pro Asp Tyr Asn Pro Glu Gly Lys Val Thr Lys Arg Lys 
    530                 535                 540                 


His Asp Asn Glu Gly Ser Gly Ser Lys Arg Pro Lys Val Glu Tyr Ser 
545                 550                 555                 560 


Glu Glu Glu Leu Lys Thr His Ile Ser Lys Gly Thr Leu Gly Lys Phe 
                565                 570                 575     


Thr Val Pro Met Leu Lys Glu Ala Cys Arg Ala Tyr Gly Leu Lys Ser 
            580                 585                 590         


Gly Leu Lys Lys Gln Glu Leu Leu Glu Ala Leu Thr Lys His Phe Gln 
        595                 600                 605             


Asp Met Val Arg Ser Gly Asn Lys Ala Ala Val Val Leu Cys Met Asp 
    610                 615                 620                 


Val Gly Phe Thr Met Ser Asn Ser Ile Pro Gly Ile Glu Ser Pro Phe 
625                 630                 635                 640 


Glu Gln Ala Lys Lys Val Ile Thr Met Phe Val Gln Arg Gln Val Phe 
                645                 650                 655     


Ala Glu Asn Lys Asp Glu Ile Ala Leu Val Leu Phe Gly Thr Asp Gly 
            660                 665                 670         


Thr Asp Asn Pro Leu Ser Gly Gly Asp Gln Tyr Gln Asn Ile Thr Val 
        675                 680                 685             


His Arg His Leu Met Leu Pro Asp Phe Asp Leu Leu Glu Asp Ile Glu 
    690                 695                 700                 


Ser Lys Ile Gln Pro Gly Ser Gln Gln Ala Asp Phe Leu Asp Ala Leu 
705                 710                 715                 720 


Ile Val Ser Met Asp Val Ile Gln His Glu Thr Ile Gly Lys Lys Phe 
                725                 730                 735     


Glu Lys Arg His Ile Glu Ile Phe Thr Asp Leu Ser Ser Arg Phe Ser 
            740                 745                 750         


Lys Ser Gln Leu Asp Ile Ile Ile His Ser Leu Lys Lys Cys Asp Ile 
        755                 760                 765             


Ser Glu Arg His Ser Ile His Trp Pro Cys Arg Leu Thr Ile Gly Ser 
    770                 775                 780                 


Asn Leu Ser Ile Arg Ile Ala Ala Tyr Lys Ser Ile Leu Gln Glu Arg 
785                 790                 795                 800 


Val Lys Lys Thr Trp Thr Val Val Asp Ala Lys Thr Leu Lys Lys Glu 
                805                 810                 815     


Asp Ile Gln Lys Glu Thr Val Tyr Cys Leu Asn Asp Asp Asp Glu Thr 
            820                 825                 830         


Glu Val Leu Lys Glu Asp Ile Ile Gln Gly Phe Arg Tyr Gly Ser Asp 
        835                 840                 845             


Ile Val Pro Phe Ser Lys Val Asp Glu Glu Gln Met Lys Tyr Lys Ser 
    850                 855                 860                 


Glu Gly Lys Cys Phe Ser Val Leu Gly Phe Cys Lys Ser Ser Gln Val 
865                 870                 875                 880 


Gln Arg Arg Phe Phe Met Gly Asn Gln Val Leu Lys Val Phe Ala Ala 
                885                 890                 895     


Arg Asp Asp Glu Ala Ala Ala Val Ala Leu Ser Ser Leu Ile His Ala 
            900                 905                 910         


Leu Asp Asp Leu Asp Met Val Ala Ile Val Arg Tyr Ala Tyr Asp Lys 
        915                 920                 925             


Arg Ala Asn Pro Gln Val Gly Val Ala Phe Pro His Ile Lys His Asn 
    930                 935                 940                 


Tyr Glu Cys Leu Val Tyr Val Gln Leu Pro Phe Met Glu Asp Leu Arg 
945                 950                 955                 960 


Gln Tyr Met Phe Ser Ser Leu Lys Asn Ser Lys Lys Tyr Ala Pro Thr 
                965                 970                 975     


Glu Ala Gln Leu Asn Ala Val Asp Ala Leu Ile Asp Ser Met Ser Leu 
            980                 985                 990         


Ala Lys Lys Asp Glu Lys Thr Asp  Thr Leu Glu Asp Leu  Phe Pro Thr 
        995                 1000                 1005             


Thr Lys  Ile Pro Asn Pro Arg  Phe Gln Arg Leu Phe  Gln Cys Leu 
    1010                 1015                 1020             


Leu His  Arg Ala Leu His Pro  Arg Glu Pro Leu Pro  Pro Ile Gln 
    1025                 1030                 1035             


Gln His  Ile Trp Asn Met Leu  Asn Pro Pro Ala Glu  Val Thr Thr 
    1040                 1045                 1050             


Lys Ser  Gln Ile Pro Leu Ser  Lys Ile Lys Thr Leu  Phe Pro Leu 
    1055                 1060                 1065             


Ile Glu  Ala Lys Lys Lys Asp  Gln Val Thr Ala Gln  Glu Ile Phe 
    1070                 1075                 1080             


Gln Asp  Asn His Glu Asp Gly  Pro Thr Ala Lys 
    1085                 1090                 


<210>  393
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Telomerase Sm7 consensus site
       (single stranded) nucleotide sequence

<400>  393
aauuuuugga                                                              10


<210>  394
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: MS2 coat protein polypeptide 
       sequence

<400>  394

Gly Ser Val Ile Asp Val Ser Ser Gln Arg Val Asn Val Gln Arg Pro 
1               5                   10                  15      


Leu Asp Ala Leu Gly Asn Ser Leu Asn Ser Pro Val Ile Ile Lys Leu 
            20                  25                  30          


Lys Gly Asp Arg Glu Phe Arg Gly Val Leu Lys Ser Phe Asp Leu His 
        35                  40                  45              


Met Asn Leu Val Leu Asn Asp Ala Glu Glu Leu Glu Asp Gly Glu Val 
    50                  55                  60                  


Thr Arg Arg Leu Gly Thr Val Leu Ile Arg Gly Asp Asn Ile Val Tyr 
65                  70                  75                  80  


Ile Ser Pro 
            


<210>  395
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: PP7 phage operator stem loop 
       nucleotide sequence

<400>  395
auaaggaguu uauauggaaa cccuua                                            26


<210>  396
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: PP7 coat protein (PCP) 
       polypeptide sequence

<400>  396

Met Ser Lys Thr Ile Val Leu Ser Val Gly Glu Ala Thr Arg Thr Leu 
1               5                   10                  15      


Thr Glu Ile Gln Ser Thr Ala Asp Arg Gln Ile Phe Glu Glu Lys Val 
            20                  25                  30          


Gly Pro Leu Val Gly Arg Leu Arg Leu Thr Ala Ser Leu Arg Gln Asn 
        35                  40                  45              


Gly Ala Lys Thr Ala Tyr Arg Val Asn Leu Lys Leu Asp Gln Ala Asp 
    50                  55                  60                  


Val Val Asp Cys Ser Thr Ser Val Cys Gly Glu Leu Pro Lys Val Arg 
65                  70                  75                  80  


Tyr Thr Gln Val Trp Ser His Asp Val Thr Ile Val Ala Asn Ser Thr 
                85                  90                  95      


Glu Ala Ser Arg Lys Ser Leu Tyr Asp Leu Thr Lys Ser Leu Val Ala 
            100                 105                 110         


Thr Ser Gln Val Glu Asp Leu Val Val Asn Leu Val Pro Leu Gly Arg 
        115                 120                 125             


<210>  397
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: SfMu Com stem loop nucleotide
       sequence

<400>  397
cugaaugccu gcgagcauc                                                    19


<210>  398
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: SfMu Com binding protein 
       polypeptide sequence

<400>  398

Met Lys Ser Ile Arg Cys Lys Asn Cys Asn Lys Leu Leu Phe Lys Ala 
1               5                   10                  15      


Asp Ser Phe Asp His Ile Glu Ile Arg Cys Pro Arg Cys Lys Arg His 
            20                  25                  30          


Ile Ile Met Leu Asn Ala Cys Glu His Pro Thr Glu Lys His Cys Gly 
        35                  40                  45              


Lys Arg Glu Lys Ile Thr His Ser Asp Glu Thr Val Arg Tyr 
    50                  55                  60          


<210>  399
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: leucine zipper polypeptide 
       sequence

<400>  399

Leu Glu Ile Arg Ala Ala Phe Leu Arg Gln Arg Asn Thr Ala Leu Arg 
1               5                   10                  15      


Thr Glu Val Ala Glu Leu Glu Gln Glu Val Gln Arg Leu Glu Asn Glu 
            20                  25                  30          


Val Ser Gln Tyr Glu Thr Arg Tyr Gly Pro Leu Gly Gly Gly Lys 
        35                  40                  45          


<210>  400
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: leucine zipper polypeptide 
       sequence

<400>  400

Leu Glu Ile Glu Ala Ala Phe Leu Glu Arg Glu Asn Thr Ala Leu Glu 
1               5                   10                  15      


Thr Arg Val Ala Glu Leu Arg Gln Arg Val Gln Arg Leu Arg Asn Arg 
            20                  25                  30          


Val Ser Gln Tyr Arg Thr Arg Tyr Gly Pro Leu Gly Gly Gly Lys 
        35                  40                  45          


<210>  401
<211>  306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: DnaE Intein-N nucleotide 
       sequence

<400>  401
tgcctgtcat acgaaaccga gatactgaca gtagaatatg gccttctgcc aatcgggaag       60

attgtggaga aacggataga atgcacagtt tactctgtcg ataacaatgg taacatttat      120

actcagccag ttgcccagtg gcacgaccgg ggagagcagg aagtattcga atactgtctg      180

gaggatggaa gtctcattag ggccactaag gaccacaaat ttatgacagt cgatggccag      240

atgctgccta tagacgaaat ctttgagcga gagttggacc tcatgcgagt tgacaacctt      300

cctaat                                                                 306


<210>  402
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: DnaE Intein-N polypeptide 
       sequence

<400>  402

Cys Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Cys Thr Val Tyr Ser 
            20                  25                  30          


Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His 
        35                  40                  45              


Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser 
    50                  55                  60                  


Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg 
                85                  90                  95      


Val Asp Asn Leu Pro Asn 
            100         


<210>  403
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: DnaE Intein-C nucleotide 
       sequence

<400>  403
atgatcaaga tagctacaag gaagtatctt ggcaaacaaa acgtttatga tattggagtc       60

gaaagagatc acaactttgc tctgaagaac ggattcatag cttctaat                   108


<210>  404
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Intein-C polypeptide sequence

<400>  404

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Asp Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala Ser Asn 
        35      


<210>  405
<211>  303
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cfa-N nucleotide sequence

<400>  405
tgcctgtctt atgataccga gatacttacc gttgaatatg gcttcttgcc tattggaaag       60

attgtcgaag agagaattga atgcacagta tatactgtag acaagaatgg tttcgtttac      120

acacagccca ttgctcaatg gcacaatcgc ggcgaacaag aagtatttga gtactgtctc      180

gaggatggaa gcatcatacg agcaactaaa gatcataaat tcatgaccac tgacgggcag      240

atgttgccaa tagatgagat attcgagcgg ggcttggatc tcaaacaagt ggatggattg      300

cca                                                                    303


<210>  406
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cfa-N polypeptide sequence

<400>  406

Cys Leu Ser Tyr Asp Thr Glu Ile Leu Thr Val Glu Tyr Gly Phe Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Glu Arg Ile Glu Cys Thr Val Tyr Thr 
            20                  25                  30          


Val Asp Lys Asn Gly Phe Val Tyr Thr Gln Pro Ile Ala Gln Trp His 
        35                  40                  45              


Asn Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser 
    50                  55                  60                  


Ile Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Thr Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Gly Leu Asp Leu Lys Gln 
                85                  90                  95      


Val Asp Gly Leu Pro 
            100     


<210>  407
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cfa-C nucleotide sequence

<400>  407
atgaagagga ctgccgatgg atcagagttt gaatctccca agaagaagag gaaagtaaag       60

ataatatctc gaaaaagtct tggtacccaa aatgtctatg atattggagt ggagaaagat      120

cacaacttcc ttctcaagaa cggtctcgta gccagcaac                             159


<210>  408
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: Cfa-C polypeptide sequence

<400>  408

Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val Lys Ile Ile Ser Arg Lys Ser Leu Gly Thr Gln Asn Val 
            20                  25                  30          


Tyr Asp Ile Gly Val Glu Lys Asp His Asn Phe Leu Leu Lys Asn Gly 
        35                  40                  45              


Leu Val Ala Ser Asn 
    50              


<210>  409
<211>  8745
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polynucleotide 
       sequence

<400>  409
aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc       60

gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta      120

gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg      180

ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga      240

cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat      300

gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa      360

gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca      420

tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca      480

tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat      540

ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg      600

actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac      660

ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg      720

cggccgctaa tacgactcac tatagggaga gccgccacca tgtccgaagt cgagttttcc      780

catgagtact ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag      840

gtgcccgtgg gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg      900

gcaatcggac tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg      960

cttgtgatgc agaattatcg acttatcgat gcgacgctgt actcgacgtt tgaaccttgc     1020

gtaatgtgcg cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc     1080

aacgccaaga cgggtgccgc aggttcactg atggacgtgc tgcatcatcc aggcatgaac     1140

caccgggtag aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgtcgt     1200

ttttttcgca tgcccaggcg ggtctttaac gcccagaaaa aagcacaatc ctctactgac     1260

tctggtggtt cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc     1320

acacccgaaa gttctggtgg ttcttctggt ggttctgaca agaagtacag catcggcctg     1380

gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc     1440

aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga     1500

gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga     1560

agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag     1620

atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag     1680

gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac     1740

gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc     1800

gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg     1860

atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg     1920

cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag     1980

gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg     2040

cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc     2100

cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac     2160

acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg     2220

tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac     2280

accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac     2340

caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag     2400

attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag     2460

gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg     2520

ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc     2580

atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt     2640

tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc     2700

tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc     2760

gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag     2820

agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc     2880

aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac     2940

gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg     3000

gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc     3060

aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc     3120

tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat     3180

gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga     3240

gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag     3300

cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc     3360

atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc     3420

aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag     3480

aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc     3540

agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa     3600

gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc     3660

acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa     3720

gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag     3780

aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac     3840

atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac     3900

gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac     3960

gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc     4020

aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc     4080

gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag     4140

cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg     4200

atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat     4260

ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg     4320

aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg     4380

tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc     4440

ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag     4500

attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc     4560

ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg     4620

ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct     4680

atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag     4740

aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg     4800

gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg     4860

gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa     4920

gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc     4980

cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc     5040

tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc     5100

gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc     5160

atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa     5220

gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc     5280

atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc     5340

accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac     5400

cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct     5460

ggtggaagcg gaggatctgg cggcagcacc aatctgagcg acatcatcga gaaagagaca     5520

ggcaagcagc tggtcatcca agagtccatc ctgatgctgc ctgaagaggt ggaagaagtg     5580

atcggcaaca agcccgagtc cgacatcctg gtgcacaccg cctacgatga gagcaccgac     5640

gagaacgtga tgctgctgac ctctgacgcc cctgagtaca agccttgggc tctcgtgatc     5700

caggacagca acggcgagaa caagatcaag atgctgagcg gcggctctgg tggctctggc     5760

ggatctacaa acctgtccga tattattgag aaagaaaccg ggaaacagct cgtgattcaa     5820

gagtctattc tcatgctccc ggaagaagtc gaggaagtca ttggaaacaa gcctgagagc     5880

gatattctgg tccatacagc ctacgacgag tctaccgatg agaatgtcat gctcctcacc     5940

agcgacgctc ccgagtataa gccatgggca cttgtcattc aggactccaa tggggaaaac     6000

aaaatcaaaa tgctcccaaa gaaaaaacgc aaggtggagg gagctgataa gcgcaccgcc     6060

gatggttccg agttcgaaag ccccaagaag aagaggaaag tctaaccggt catcatcacc     6120

atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat     6180

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     6240

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     6300

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     6360

gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt     6420

cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt     6480

atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg     6540

cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg     6600

gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc     6660

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     6720

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     6780

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     6840

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     6900

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     6960

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     7020

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     7080

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     7140

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     7200

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     7260

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     7320

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     7380

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     7440

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     7500

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     7560

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     7620

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     7680

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     7740

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     7800

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     7860

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     7920

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     7980

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     8040

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     8100

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     8160

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     8220

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     8280

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     8340

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     8400

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     8460

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     8520

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     8580

catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc     8640

tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc     8700

ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aattt                     8745


<210>  410
<211>  8745
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic polynucleotide 
       sequence

<400>  410
aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc       60

gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta      120

gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg      180

ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga      240

cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat      300

gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa      360

gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca      420

tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca      480

tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat      540

ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg      600

actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac      660

ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg      720

cggccgctaa tacgactcac tatagggaga gccgccacca tgtccgaagt cgagttttcc      780

catgagtact ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag      840

gtgcccgtgg gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg      900

gcaatcggac tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg      960

cttgtgatgc agaattatcg actttatgat gcgacgctgt actcgacgtt tgaaccttgc     1020

gtaatgtgcg cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc     1080

aacgccaaga cgggtgccgc aggttcactg atggacgtgc tgcatcatcc aggcatgaac     1140

caccgggtag aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgtcgt     1200

ttttttcgca tgcccaggcg tgtctttaac gcccagaaaa aagcacaatc ctctactgac     1260

tctggtggtt cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc     1320

acacccgaaa gttctggtgg ttcttctggt ggttctgaca agaagtacag catcggcctg     1380

gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc     1440

aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga     1500

gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga     1560

agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag     1620

atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag     1680

gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac     1740

gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc     1800

gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg     1860

atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg     1920

cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag     1980

gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg     2040

cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc     2100

cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac     2160

acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg     2220

tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac     2280

accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac     2340

caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag     2400

attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag     2460

gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg     2520

ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc     2580

atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt     2640

tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc     2700

tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc     2760

gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag     2820

agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc     2880

aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac     2940

gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg     3000

gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc     3060

aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc     3120

tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat     3180

gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga     3240

gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag     3300

cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc     3360

atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc     3420

aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag     3480

aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc     3540

agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa     3600

gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc     3660

acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa     3720

gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag     3780

aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac     3840

atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac     3900

gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac     3960

gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc     4020

aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc     4080

gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag     4140

cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg     4200

atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat     4260

ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg     4320

aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg     4380

tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc     4440

ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag     4500

attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc     4560

ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg     4620

ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct     4680

atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag     4740

aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg     4800

gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg     4860

gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa     4920

gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc     4980

cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc     5040

tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc     5100

gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc     5160

atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa     5220

gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc     5280

atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc     5340

accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac     5400

cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct     5460

ggtggaagcg gaggatctgg cggcagcacc aatctgagcg acatcatcga gaaagagaca     5520

ggcaagcagc tggtcatcca agagtccatc ctgatgctgc ctgaagaggt ggaagaagtg     5580

atcggcaaca agcccgagtc cgacatcctg gtgcacaccg cctacgatga gagcaccgac     5640

gagaacgtga tgctgctgac ctctgacgcc cctgagtaca agccttgggc tctcgtgatc     5700

caggacagca acggcgagaa caagatcaag atgctgagcg gcggctctgg tggctctggc     5760

ggatctacaa acctgtccga tattattgag aaagaaaccg ggaaacagct cgtgattcaa     5820

gagtctattc tcatgctccc ggaagaagtc gaggaagtca ttggaaacaa gcctgagagc     5880

gatattctgg tccatacagc ctacgacgag tctaccgatg agaatgtcat gctcctcacc     5940

agcgacgctc ccgagtataa gccatgggca cttgtcattc aggactccaa tggggaaaac     6000

aaaatcaaaa tgctcccaaa gaaaaaacgc aaggtggagg gagctgataa gcgcaccgcc     6060

gatggttccg agttcgaaag ccccaagaag aagaggaaag tctaaccggt catcatcacc     6120

atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat     6180

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     6240

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     6300

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     6360

gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt     6420

cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt     6480

atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg     6540

cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg     6600

gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc     6660

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     6720

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     6780

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     6840

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     6900

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     6960

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     7020

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     7080

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     7140

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     7200

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     7260

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     7320

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     7380

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     7440

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     7500

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     7560

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     7620

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     7680

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     7740

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     7800

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     7860

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     7920

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     7980

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     8040

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     8100

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     8160

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     8220

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     8280

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     8340

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     8400

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     8460

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     8520

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     8580

catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc     8640

tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc     8700

ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aattt                     8745


<210>  411
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of artificial sequence: synthetic oligonucleotide 
       sequence


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is 2'-deoxy-8-azanebularine

<400>  411
gctcggctnc gga                                                          13


