                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  Hybrid Receptors With Multiple Transcriptional Regulators

<130>  048536-695001WO

<140>  Herewith
<141>  Herewith

<150>  US 63/165,442
<151>  2021-03-24

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Signal Sequence

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  2
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Myc Tag

<400>  2

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  3
<211>  128
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Lag_17

<400>  3

Met Ala Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Ser 
            20                  25                  30          


Met Ala Ala Met Ser Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ala Gly Ile Ser Arg Ser Ala Gly Ser Ala Val His Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Val Arg Thr Ser Gly Phe Phe Gly Ser Ile Pro Arg Thr 
            100                 105                 110         


Gly Thr Ala Phe Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


<210>  4
<211>  326
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Notch_Core

<400>  4

Ile Leu Asp Tyr Ser Phe Thr Gly Gly Ala Gly Arg Asp Ile Pro Pro 
1               5                   10                  15      


Pro Gln Ile Glu Glu Ala Cys Glu Leu Pro Glu Cys Gln Val Asp Ala 
            20                  25                  30          


Gly Asn Lys Val Cys Asn Leu Gln Cys Asn Asn His Ala Cys Gly Trp 
        35                  40                  45              


Asp Gly Gly Asp Cys Ser Leu Asn Phe Asn Asp Pro Trp Lys Asn Cys 
    50                  55                  60                  


Thr Gln Ser Leu Gln Cys Trp Lys Tyr Phe Ser Asp Gly His Cys Asp 
65                  70                  75                  80  


Ser Gln Cys Asn Ser Ala Gly Cys Leu Phe Asp Gly Phe Asp Cys Gln 
                85                  90                  95      


Leu Thr Glu Gly Gln Cys Asn Pro Leu Tyr Asp Gln Tyr Cys Lys Asp 
            100                 105                 110         


His Phe Ser Asp Gly His Cys Asp Gln Gly Cys Asn Ser Ala Glu Cys 
        115                 120                 125             


Glu Trp Asp Gly Leu Asp Cys Ala Glu His Val Pro Glu Arg Leu Ala 
    130                 135                 140                 


Ala Gly Thr Leu Val Leu Val Val Leu Leu Pro Pro Asp Gln Leu Arg 
145                 150                 155                 160 


Asn Asn Ser Phe His Phe Leu Arg Glu Leu Ser His Val Leu His Thr 
                165                 170                 175     


Asn Val Val Phe Lys Arg Asp Ala Gln Gly Gln Gln Met Ile Phe Pro 
            180                 185                 190         


Tyr Tyr Gly His Glu Glu Glu Leu Arg Lys His Pro Ile Lys Arg Ser 
        195                 200                 205             


Thr Val Gly Trp Ala Thr Ser Ser Leu Leu Pro Gly Thr Ser Gly Gly 
    210                 215                 220                 


Arg Gln Arg Arg Glu Leu Asp Pro Met Asp Ile Arg Gly Ser Ile Val 
225                 230                 235                 240 


Tyr Leu Glu Ile Asp Asn Arg Gln Cys Val Gln Ser Ser Ser Gln Cys 
                245                 250                 255     


Phe Gln Ser Ala Thr Asp Val Ala Ala Phe Leu Gly Ala Leu Ala Ser 
            260                 265                 270         


Leu Gly Ser Leu Asn Ile Pro Tyr Lys Ile Glu Ala Val Lys Ser Glu 
        275                 280                 285             


Pro Val Glu Pro Pro Leu Pro Ser Gln Leu His Leu Met Tyr Val Ala 
    290                 295                 300                 


Ala Ala Ala Phe Val Leu Leu Phe Phe Val Gly Cys Gly Val Leu Leu 
305                 310                 315                 320 


Ser Arg Lys Arg Arg Arg 
                325     


<210>  5
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  CD3-Zeta

<400>  5

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  6
<211>  42
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  4-1BB co-stimulatory domain

<400>  6

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  7
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  CD28 co-stimulatory domain

<400>  7

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  8
<211>  211
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Gal4-VP64

<400>  8

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Ala Ala Ala Gly Gly Ser Gly Gly Ser Gly Gly Ser Asp 
145                 150                 155                 160 


Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp 
                165                 170                 175     


Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp 
            180                 185                 190         


Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met 
        195                 200                 205             


Leu Gly Ser 
    210     


<210>  9
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  GS Linker

<400>  9

Gly Ser Gly Ser Gly Ser Gly Ser 
1               5               


<210>  10
<211>  242
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  CD19 scFv

<400>  10

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  11
<211>  299
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Notch_Core_Linking_polypeptide

<400>  11

Ile Leu Asp Tyr Ser Phe Thr Gly Gly Ala Gly Arg Asp Ile Pro Pro 
1               5                   10                  15      


Pro Gln Ile Glu Glu Ala Cys Glu Leu Pro Glu Cys Gln Val Asp Ala 
            20                  25                  30          


Gly Asn Lys Val Cys Asn Leu Gln Cys Asn Asn His Ala Cys Gly Trp 
        35                  40                  45              


Asp Gly Gly Asp Cys Ser Leu Asn Phe Asn Asp Pro Trp Lys Asn Cys 
    50                  55                  60                  


Thr Gln Ser Leu Gln Cys Trp Lys Tyr Phe Ser Asp Gly His Cys Asp 
65                  70                  75                  80  


Ser Gln Cys Asn Ser Ala Gly Cys Leu Phe Asp Gly Phe Asp Cys Gln 
                85                  90                  95      


Leu Thr Glu Gly Gln Cys Asn Pro Leu Tyr Asp Gln Tyr Cys Lys Asp 
            100                 105                 110         


His Phe Ser Asp Gly His Cys Asp Gln Gly Cys Asn Ser Ala Glu Cys 
        115                 120                 125             


Glu Trp Asp Gly Leu Asp Cys Ala Glu His Val Pro Glu Arg Leu Ala 
    130                 135                 140                 


Ala Gly Thr Leu Val Leu Val Val Leu Leu Pro Pro Asp Gln Leu Arg 
145                 150                 155                 160 


Asn Asn Ser Phe His Phe Leu Arg Glu Leu Ser His Val Leu His Thr 
                165                 170                 175     


Asn Val Val Phe Lys Arg Asp Ala Gln Gly Gln Gln Met Ile Phe Pro 
            180                 185                 190         


Tyr Tyr Gly His Glu Glu Glu Leu Arg Lys His Pro Ile Lys Arg Ser 
        195                 200                 205             


Thr Val Gly Trp Ala Thr Ser Ser Leu Leu Pro Gly Thr Ser Gly Gly 
    210                 215                 220                 


Arg Gln Arg Arg Glu Leu Asp Pro Met Asp Ile Arg Gly Ser Ile Val 
225                 230                 235                 240 


Tyr Leu Glu Ile Asp Asn Arg Gln Cys Val Gln Ser Ser Ser Gln Cys 
                245                 250                 255     


Phe Gln Ser Ala Thr Asp Val Ala Ala Phe Leu Gly Ala Leu Ala Ser 
            260                 265                 270         


Leu Gly Ser Leu Asn Ile Pro Tyr Lys Ile Glu Ala Val Lys Ser Glu 
        275                 280                 285             


Pro Val Glu Pro Pro Leu Pro Ser Gln Leu His 
    290                 295                 


<210>  12
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Notch_Core_TMD

<400>  12

Leu Met Tyr Val Ala Ala Ala Ala Phe Val Leu Leu Phe Phe Val Gly 
1               5                   10                  15      


Cys Gly Val Leu Leu 
            20      


<210>  13
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<220>
<221>  MISC_FEATURE
<223>  Cleavage sequence of MMP-9

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Leu, Ile, Val, Phe, Trp, Tyr, Val, Met, or Pro

<400>  13

Pro Xaa Xaa Xaa 
1               


<210>  14
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<220>
<221>  MISC_FEATURE
<223>  Cleavage sequence of MMP-9

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is Leu, Ile, Val, Phe, Trp, Tyr, Val, Met, or Pro

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Ser or Thr

<400>  14

Pro Xaa Xaa Xaa Xaa 
1               5   


<210>  15
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<220>
<221>  MISC_FEATURE
<223>  Cleavage sequence of MMP-9

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Leu or Gln

<400>  15

Pro Xaa Gly Met Thr Ser 
1               5       


<210>  16
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<220>
<221>  MISC_FEATURE
<223>  Cleavage sequence of MMP-9


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Leu or Gln

<400>  16

Pro Xaa Gly Met Thr 
1               5   


<210>  17
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<220>
<221>  MISC_FEATURE
<223>  Cleavage sequence of MMP-9

<400>  17

Glu Asn Leu Tyr Thr Gln Ser 
1               5           


