
                                SEQUENCE LISTING

<110> Archer Daniels Midland Company

<120> THERMOSTABLE D-GLUCARATE DEHYDRATASE
  THAT IS RESISTANT TO INHIBITION BY TARTRATE
  

<130> CP.0188.US00

<160> 148

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 1
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 2
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 2
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 3
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 3
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 4
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 4
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Asn Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 5
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 5
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Gly Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Ala Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 6
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 6
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Arg Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 7
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 7
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Arg Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 8
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 8
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Ser Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Ala Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 9
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 9
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Arg Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 10
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 10
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 11
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 11
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Asp Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 12
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 12
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Asn Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Ala Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 13
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 13
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asp Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 14
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 14
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Arg Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 15
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 15
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Ser Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 16
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 16
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Met Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Ile Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asn Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 17
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 17
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile His Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Asp Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 18
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 18
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Val Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Lys Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 19
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 19
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 20
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 20
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Val Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Ile Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 21
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 21
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Ile Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 22
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 22
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Arg Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 23
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 23
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Val Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 24
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 24
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asn Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 25
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 25
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 26
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 26
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 27
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 27
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 28
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 28
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 29
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 29
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Arg Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 30
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 30
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 31
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 31
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 32
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 32
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 33
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 33
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Ala Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 34
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 34
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala His
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 35
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 35
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Arg Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 36
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 36
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 37
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 37
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 38
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 38
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Arg Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 39
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 39
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Ser Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 40
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 40
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Arg Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 41
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 41
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 42
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 42
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 43
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 43
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 44
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 44
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Arg Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Arg Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 45
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 45
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Val Glu Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 46
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 46
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 47
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 47
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Arg Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 48
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 48
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asp Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 49
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 49
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 50
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 50
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Arg Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 51
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 51
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Val Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 52
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 52
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Ala Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Lys Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 53
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 53
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Ala Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 54
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 54
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Ala Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 55
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 55
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 56
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 56
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Arg Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Arg Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 57
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 57
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala His
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Ala Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 58
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 58
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Lys Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 59
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 59
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 60
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 60
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 61
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 61
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 62
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 62
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 63
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 63
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 64
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 64
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 65
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 65
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala His
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Lys Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 66
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 66
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Arg Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Ala Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 67
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 67
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Ala Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 68
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 68
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Asn Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 69
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 69
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Arg Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 70
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 70
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Gln Ser Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 71
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 71
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Lys Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Ala Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 72
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 72
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Arg Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 73
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 73
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 74
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 74
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Asn Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 75
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 75
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Arg Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Arg Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Asn Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 76
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 76
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 77
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 77
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Arg Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Lys Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 78
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 78
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 79
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 79
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Lys Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Ala Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 80
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 80
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Ile Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Lys Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 81
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 81
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Lys Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 82
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 82
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 83
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 83
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Arg Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Ser Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Val Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 84
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 84
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Leu Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Lys Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 85
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 85
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Glu Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Lys Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 86
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 86
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Lys Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Arg Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 87
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 87
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 88
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 88
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Asp Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 89
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 89
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Glu Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Lys Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 90
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 90
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 91
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 91
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Leu
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 92
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 92
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Tyr Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Ala Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 93
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 93
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Ala Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 94
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 94
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 95
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 95
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Lys Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 96
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 96
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Ile Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 97
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 97
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Ala Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 98
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 98
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Ala Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 99
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 99
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Glu Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 100
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 100
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ser Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Val Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 101
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 101
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Glu Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 102
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 102
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Ser Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 103
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 103
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 104
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 104
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Lys Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Ala
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 105
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 105
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 106
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 106
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Arg Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys Asn Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 107
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 107
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu Ala Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 108
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 108
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys Asn Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 109
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 109
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Ser Ser Glu Trp Gly Arg Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 110
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 110
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 111
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 111
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ser Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 112
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 112
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Thr Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 113
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 113
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Glu Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 114
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 114
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 115
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 115
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 116
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 116
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu Ala Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 117
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 117
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Val Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys Asn Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 118
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 118
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 119
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 119
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Thr Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Ala Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 120
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 120
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Thr Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Glu Asp Asp Glu Thr Ser Glu Trp Gly Arg Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 121
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 121
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Arg Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 122
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 122
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Ala Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 123
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 123
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Arg Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ser Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 124
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 124
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Val Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Ala
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 125
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 125
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ser Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 126
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 126
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ser Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu Ala Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys Asn Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 127
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 127
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Glu Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 128
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 128
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu Ala Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Ala
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 129
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 129
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Val Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 130
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 130
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Ala Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Ser Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 131
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 131
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Val Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Glu Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 132
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 132
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Ser Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 133
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 133
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Ala Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 134
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 134
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Ala Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Glu Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 135
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 135
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Asp Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 136
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 136
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Ala Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Arg Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 137
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 137
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Ile Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Arg Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 138
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 138
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Ala Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Glu Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 139
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 139
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Asp Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asp Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 140
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> glucarate dehydratase variant

<400> 140
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Asn Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Thr Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ile Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Glu
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Lys Ile Thr His Met Pro Val Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Arg Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Ile Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Glu Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 141
<211> 443
<212> PRT
<213> Enterococcus sp.

<220> 
<223> glucarate dehydratase variant

<400> 141
Met Met Ile Pro Lys Ile Val Lys Met Glu Val Val Pro Val Ala Gly
 1               5                  10                  15
His Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Pro Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Thr Asp Asp Thr Gly Asn Thr Gly Leu
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Arg Gln Thr Leu Glu Asp Cys
    50                  55                  60
Arg Glu Leu Val Val Gly Arg Thr Leu Gly Glu Tyr Lys Asn Ile Val
65                  70                  75                  80
Lys Glu Ile His Glu Arg Tyr Ala Gln Leu Asp Ala Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Leu Arg Thr Thr Val His Val Met Thr Ala Val
            100                 105                 110
Glu Ala Pro Leu Leu Asp Leu Leu Gly Gln His Met Gln Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Glu Gly Gln Val Arg Glu Ser Val Lys Phe Leu
    130                 135                 140
Gly Tyr Leu Phe Tyr Val Ala Asp Arg Asp Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Ala Ser Asp Asn Thr Leu Asp Asp Ser Trp Gly Ala Leu Arg Arg Glu
                165                 170                 175
Glu Ala Met Thr Pro Glu Ala Ile Val Lys Leu Ala Lys Ala Ala Tyr
            180                 185                 190
Glu Arg Tyr Gly Phe Glu Asp Phe Lys Leu Lys Gly Gly Val Leu Ser
        195                 200                 205
Gly Gln Glu Glu Val Gln Thr Val Lys Ala Leu Lys Lys Glu Phe Pro
    210                 215                 220
Asn Ala Arg Ile Thr Leu Asp Pro Asn Gly Gly Trp Leu Leu Asp Asp
225                 230                 235                 240
Ala Ile Ala Leu Cys Lys Asp Leu His Gly Val Leu Ala Tyr Val Glu
                245                 250                 255
Asp Pro Cys Gly Ala Glu Gly Arg Phe Ser Gly Arg Glu Thr Met Ala
            260                 265                 270
Glu Phe Arg Arg Lys Thr Gly Leu Pro Thr Ala Thr Asn Met Val Ala
        275                 280                 285
Thr Asp Trp Arg Glu Met Lys Ala Ala Ile Arg Leu Asp Ser Val Thr
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ser Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Asp Leu Asp Leu Thr Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala His Thr Ala Ala
            340                 345                 350
Ala Ala Pro Gly Glu Val Asn Ala Ala Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Glu Gly Leu Thr Lys Asn Pro His Gln Ile Lys Asn Gly Glu
    370                 375                 380
Ile Thr Ile Pro Arg Asn Ser His Gly Leu Gly Leu Glu Leu Asp Arg
385                 390                 395                 400
Glu Lys Leu Asp Ala Ala His Gln Leu Tyr Leu Lys Glu Gly Leu Gly
                405                 410                 415
Ala Arg Asn Asp Ala Ile Ala Met Gln Tyr Leu Ile Pro Gly Trp Thr
            420                 425                 430
Phe Asp Asn Lys Lys Pro Ala Leu Val Arg Glu
        435                 440

<210> 142
<211> 443
<212> PRT
<213> Oribacterium sp.

<220> 
<223> glucarate dehydratase variant

<400> 142
Met Ile Pro Thr Ile Thr Lys Met Glu Val Phe Pro Val Ala Gly His
 1               5                  10                  15
Asp Cys Met Glu Leu Asn Leu Ser Gly Ala His Ala Pro Tyr Phe Thr
            20                  25                  30
Arg Asn Ile Val Ile Leu Thr Asp Ser Asn Gly Val Glu Gly Val Gly
        35                  40                  45
Glu Val Pro Gly Gly Gln Lys Ile Thr Asn Ala Leu Glu Ala Val Lys
    50                  55                  60
Asp Leu Val Ile Gly Thr Lys Ile Ser Asp Tyr Lys Asn Thr Leu Asn
65                  70                  75                  80
Lys Val Arg Ala Trp Leu Asp Glu Asn Ile Lys Asp Asp Val Arg Gly
                85                  90                  95
Leu Gln Thr Phe Asp Leu Arg Thr Gly Val His Val Val Thr Ala Ile
            100                 105                 110
Glu Ala Pro Leu Leu Asp Leu Met Gly Lys Phe Leu Glu Val Pro Ala
        115                 120                 125
Ala Ser Leu Met Gly Asp Gly Ile Gln Arg Thr Ser Val Arg Phe Leu
    130                 135                 140
Ser Tyr Leu Phe Tyr Val Gly Asp Arg Lys Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Glu Glu Glu Pro Asp Ser Asp Cys Asp Trp Tyr Arg Leu Arg His Glu
                165                 170                 175
Glu Ala Met Asp Pro Glu His Ile Val Ala Leu Ala Lys Ala Thr His
            180                 185                 190
Glu Lys Tyr Gly Phe Glu Asp Phe Lys Leu Lys Gly Gly Val Leu Thr
        195                 200                 205
Pro Asp Glu Glu Val Lys Ala Val Thr Ala Ile Lys Glu Ala Phe Pro
    210                 215                 220
Asn Ala Arg Val Asp Leu Asp Pro Asn Gly Cys Trp Ser Leu Ala Glu
225                 230                 235                 240
Ala Val Arg Val Ala Pro Ala Leu Lys Lys Val Leu Ala Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Ala Glu Asn Gly Phe Ser Gly Arg Glu Ile Met Ala
            260                 265                 270
Glu Phe Lys Gln Glu Thr Gly Met Pro Thr Ala Thr Asn Met Ile Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Arg His Cys Ile Gln Leu Lys Ser Val Asp
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ser Val Arg
305                 310                 315                 320
Val Gly Gln Leu Cys Asn Asp Phe Gly Leu Met Trp Gly Cys His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Gln Cys Ala Ala
            340                 345                 350
Ala Val Pro Gly Lys Leu Asn Gly Ile Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Glu Gly Arg Glu Arg Leu Thr Lys Asn Pro Met Gln Ile Val Gly Gly
    370                 375                 380
Cys Ile Asn Asp Ile Asp Lys Thr Val Gly Leu Gly Ile Glu Val Asp
385                 390                 395                 400
Arg Glu Gln Ile Met Lys Ala Asn Lys Leu Tyr Val Asp Asn Cys Leu
                405                 410                 415
Gly Ala Arg Asp Asp Ala Ile Gly Met Gln Tyr Leu Ile Pro Gly Trp
            420                 425                 430
Lys Phe Asp Asn Lys Arg Pro Cys Met Ile Arg
        435                 440

<210> 143
<211> 441
<212> PRT
<213> Halomonas sp.

<220> 
<223> glucarate dehydratase variant

<400> 143
Met Phe Pro Thr Ile Lys Lys Met Arg Val Val Pro Val Ala Gly Tyr
 1               5                  10                  15
Asp Gly Phe Leu Leu Asn Leu Ser Gly Gly His Ala Pro Trp Phe Ile
            20                  25                  30
Arg Cys Val Leu Ile Leu Glu Asp Asp Ala Gly Asn Lys Gly Val Gly
        35                  40                  45
Glu Ile Pro Ser Ser Asp Gly Ile Ile Lys Gly Leu Glu Gln Cys Arg
    50                  55                  60
Ser Leu Val Glu Gly Ser Gln Val Asn Asn Val Lys Gln Thr Leu Asn
65                  70                  75                  80
Gln Ala Arg Gln Ile Leu Ala Gln Asn Gly Arg Glu Glu Arg Gly Arg
                85                  90                  95
Gln Thr Phe Asp Leu Arg Val Ala Val His Val Ile Thr Ala Ile Glu
            100                 105                 110
Ser Ala Leu Phe Asp Leu Phe Gly Gln Ala Leu Gly Met Pro Val Ala
        115                 120                 125
Asp Leu Leu Gly Gln Tyr Gly Arg Gln Arg Asp Glu Val Glu Ala Leu
    130                 135                 140
Gly Tyr Leu Phe Leu Leu Gly Asp Pro Glu Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Pro Lys Ala Glu Asn Pro Lys Asp Thr Trp Asp Glu Val Arg Tyr Lys
                165                 170                 175
Glu Ala Leu Thr Pro Asp Ala Val Ala Asn Leu Ala Lys Ala Ala Tyr
            180                 185                 190
Asp Arg Tyr Gly Phe Lys Asp Phe Lys Leu Lys Gly Gly Val Leu Arg
        195                 200                 205
Gly Glu Glu Glu Ala Asp Cys Ile Arg Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Glu Ala Arg Leu Thr Leu Asp Pro Asn Gly Ala Trp Lys Leu Asp Glu
225                 230                 235                 240
Ala Val Arg Val Leu Asp Pro Ile Lys His Leu Leu Ser Tyr Ala Glu
                245                 250                 255
Asp Pro Cys Gly Gln Glu Glu Ser Tyr Ser Gly Arg Glu Thr Met Ala
            260                 265                 270
Glu Phe Lys Lys Arg Thr Gly Leu Pro Thr Ala Thr Asn Met Ile Ala
        275                 280                 285
Thr Asp Phe Lys Gln Leu Gln Tyr Ala Val Gln Leu Asn Ser Val Asp
    290                 295                 300
Ile Pro Leu Ala Asp Cys His Phe Trp Thr Met Gln Gly Ala Val Met
305                 310                 315                 320
Val Gly Glu Leu Cys Asn Glu Trp Gly Met Thr Trp Gly Ser His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Met Thr His Val Ala Ala
            340                 345                 350
Ala Cys Pro Gly Glu Ile Thr Ala Ile Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Arg Ile Thr Lys Glu Pro Phe Gln Ile Arg Asp Gly Lys
    370                 375                 380
Leu Thr Val Pro Lys Thr Pro Gly Leu Gly Val Glu Leu Asp Glu Glu
385                 390                 395                 400
Lys Leu Lys Glu Ala His Ala Leu Tyr Lys Ser Leu Asp Val Thr Gln
                405                 410                 415
Arg Asn Asp Ala Met Ala Met Gln Phe Leu Ile Pro Gly Trp Glu Phe
            420                 425                 430
Asp Pro Lys Lys Pro Ala Leu Val Arg
        435                 440

<210> 144
<211> 444
<212> PRT
<213> F. plautii

<220> 
<223> glucarate dehydratase variant

<400> 144
Met Ala Asn Thr Pro Lys Val Ala Ala Met Glu Val Tyr Pro Val Ala
 1               5                  10                  15
Gly Lys Asp Cys Met Glu Leu Asn Leu Ser Gly Ala His Ala Pro Tyr
            20                  25                  30
Phe Thr Arg Asn Ile Val Val Leu Thr Asp Asp Arg Gly Asn Thr Gly
        35                  40                  45
Ile Gly Glu Val Pro Gly Gly Gln Lys Ile Thr Arg Ala Leu Glu Asn
    50                  55                  60
Val Lys His Leu Val Glu Gly Thr Ser Val Ala Asp Tyr Lys Met Thr
65                  70                  75                  80
Leu Arg Arg Leu Asn Glu Trp Leu Thr Ala Asn Ile Lys Asp Asp Val
                85                  90                  95
Arg Gly Gln Gln Thr Phe Asp Leu Arg Thr Gly Val His Val Val Thr
            100                 105                 110
Ala Val Glu Thr Pro Leu Leu Asp Leu Leu Gly Gln Tyr Leu Glu Val
        115                 120                 125
Pro Met Ala Ala Leu Leu Gly Asp Gly Ile Gln Arg Asp Arg Val Arg
    130                 135                 140
Phe Leu Ser Tyr Leu Phe Tyr Val Gly Asp Arg Lys Lys Thr Asp Leu
145                 150                 155                 160
Pro Tyr His Ser Glu Pro Asp Ser Asp Cys Asp Trp Tyr Arg Leu Arg
                165                 170                 175
His Glu Glu Ala Leu Thr Pro Asp Ala Ile Val Ala Leu Ala Arg Ala
            180                 185                 190
Thr Tyr Glu Lys Tyr Gly Phe Glu Asp Phe Lys Leu Lys Gly Gly Val
        195                 200                 205
Leu Glu Gly Arg Glu Glu Leu Lys Ala Val Gln Ala Leu Lys Glu Ala
    210                 215                 220
Phe Pro Lys Ala Arg Ile Thr Leu Asp Pro Asn Gly Gly Trp Leu Leu
225                 230                 235                 240
Lys Asp Ala Leu Ala Leu Ala Pro Glu Leu Lys Lys Val Leu Ala Tyr
                245                 250                 255
Cys Glu Asp Pro Cys Gly Ala Glu Gly Arg Phe Ser Gly Arg Glu Ile
            260                 265                 270
Met Ala Glu Phe Arg Gln Glu Ser Gly Met Pro Thr Ala Thr Asn Met
        275                 280                 285
Ile Asp Thr Asp Trp Arg Glu Met Arg His Cys Ile Ala Leu Arg Ser
    290                 295                 300
Val Asp Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Ala Gly Ser
305                 310                 315                 320
Val Arg Val Gly Gln Met Cys Asn Asp Phe Gly Leu Met Trp Gly Cys
                325                 330                 335
His Ser Asn Asn His Phe Asp Ile Ser Leu Ala Met Met Thr Gln Cys
            340                 345                 350
Ala Ala Ala Val Pro Gly Lys Leu Asn Gly Ile Asp Thr His Trp Ile
        355                 360                 365
Trp Gln Glu Gly Leu Glu Arg Leu Thr Arg Ala Pro Leu Gln Ile Val
    370                 375                 380
Asp Gly Cys Ile Asn Leu Pro Glu Arg Pro Gly Leu Gly Val Glu Leu
385                 390                 395                 400
Asp Arg Glu Gln Leu Gln Lys Ala His Gln Leu Tyr Leu Asp Asn Cys
                405                 410                 415
Leu Gly Gly Arg Asp Asp Ala Val Gly Met Gln Tyr Leu Ile Pro Gly
            420                 425                 430
Trp Thr Phe Asp Pro Lys Arg Pro Cys Leu Val Arg
        435                 440

<210> 145
<211> 431
<212> PRT
<213> Streptococcus Sp.CS113

<220> 
<223> glucarate dehydratase variant

<400> 145
Met Thr Arg Asp Leu Thr Ile Thr Ala Val Arg Leu Thr Pro Ile Leu
 1               5                  10                  15
Val Ala Asp Pro Pro Leu Leu Asn Thr Gln Gly Val His Gln Pro Tyr
            20                  25                  30
Thr Pro Arg Leu Ile Val Glu Val Glu Thr Ala Asp Gly Val Thr Gly
        35                  40                  45
Val Gly Glu Thr Tyr Gly Asp Ala Lys Tyr Leu Glu Leu Ala Arg Pro
    50                  55                  60
Phe Ala Asp Lys Leu Val Gly Arg Gln Val Ser Asp Leu Asn Gly Leu
65                  70                  75                  80
Phe Thr Leu Ala Asp Glu Val Ala Val Asp Ser Ser Arg Val Ser Gly
                85                  90                  95
Gln Val Asp Val Gly Gly Leu Arg Gly Val Gln Thr Ala Asp Lys Leu
            100                 105                 110
Arg Leu Ser Val Val Ser Gly Phe Glu Val Ala Cys Leu Asp Ala Leu
        115                 120                 125
Gly Lys Ala Leu Gly Leu Pro Val His Ala Leu Leu Gly Gly Lys Val
    130                 135                 140
Arg Asp Ala Val Glu Tyr Ser Ala Tyr Leu Phe Tyr Lys Trp Ser Asp
145                 150                 155                 160
His Pro Glu Gly Val Ala Ser Glu Lys Asp Asp Trp Gly Ala Ala Val
                165                 170                 175
Asp Pro Ala Gly Val Val Ala Gln Ala Arg Ala Phe Thr Glu Arg Tyr
            180                 185                 190
Gly Phe Thr Ser Phe Lys Leu Lys Gly Gly Val Phe Pro Pro Asp Glu
        195                 200                 205
Glu Ile Ala Ala Val Lys Ala Leu Ala Glu Ala Phe Pro Gly His Pro
    210                 215                 220
Leu Arg Leu Asp Pro Asn Gly Ala Trp Ser Val Glu Thr Ser Leu Lys
225                 230                 235                 240
Val Ala Ala Glu Leu Gly Asp Val Leu Glu Tyr Leu Glu Asp Pro Ala
                245                 250                 255
Leu Gly Thr Pro Ala Met Ala Glu Val Ala Ala Arg Thr Gly Val Pro
            260                 265                 270
Leu Ala Thr Asn Met Cys Val Thr Thr Phe Ala Glu Ile Gln Glu Ala
        275                 280                 285
Phe Thr Lys Gly Ala Val Gln Val Val Leu Ser Asp His His Tyr Trp
    290                 295                 300
Gly Gly Leu Arg Asn Thr Gln Gln Leu Ala Ala Val Cys Arg Thr Phe
305                 310                 315                 320
Gly Val Gly Val Ser Met His Ser Asn Thr His Leu Gly Ile Ser Leu
                325                 330                 335
Ala Ala Met Thr His Val Ala Ala Thr Val Pro Asp Leu His His Ala
            340                 345                 350
Cys Asp Ser His Tyr Pro Trp Gln Ser Glu Asp Val Leu Thr Glu Arg
        355                 360                 365
Leu Thr Phe Asp Gly Gly Lys Val Ala Val Ser Asp Ala Pro Gly Leu
    370                 375                 380
Gly Val Glu Leu Asp Arg Glu Arg Leu Ala Phe Leu His Arg Arg Trp
385                 390                 395                 400
Leu Asp Asp Asp Gly Thr Leu Arg Asp Arg Asp Asp Ala Ala Ala Met
                405                 410                 415
Arg Val Ala Asp Pro Glu Trp Val Thr Pro Ser Val Pro Arg Trp
            420                 425                 430

<210> 146
<211> 442
<212> PRT
<213> L. pentous

<220> 
<223> glucarate dehydratase variant

<400> 146
Met Ser Ile Pro Lys Ile Val Asp Met Leu Val Tyr Pro Val Ala Gly
 1               5                  10                  15
Tyr Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Gly Ala Phe Phe
            20                  25                  30
Thr Arg Asn Ile Val Ile Leu Lys Ala Asp Asn Gly Gln Val Gly Val
        35                  40                  45
Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Glu Thr Leu Asn Ala Ser
    50                  55                  60
Lys Asp Leu Val Ile Asn Glu Ser Ile Gly Asn Tyr Lys Ala Ala Leu
65                  70                  75                  80
Gln Lys Ile Arg Ala Lys Phe Ser Tyr Leu Asp Gln Gly Gly Arg Gly
                85                  90                  95
Asn Gln Thr Phe Asp Gln Arg Ile Met Val His Ala Leu Thr Ala Ile
            100                 105                 110
Glu Thr Ala Phe Leu Asp Leu Leu Gly Lys His Phe His Val Pro Val
        115                 120                 125
Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Glu Lys Val Gly Val Leu
    130                 135                 140
Gly Tyr Leu Phe Trp Val Gly Asp Ser Ser Lys Thr Asp Leu Pro Tyr
145                 150                 155                 160
Leu Gln Asp Asp Asp Glu Thr Ser Glu Trp Gly Lys Leu Arg Arg Lys
                165                 170                 175
Pro Val Met Asp Ala Asp Ala Leu Val Lys Leu Ala Gln Ala Ala Tyr
            180                 185                 190
Asp Lys Tyr Gly Phe Lys Thr Phe Lys Leu Lys Gly Gly Val Phe Asp
        195                 200                 205
Gly Glu Thr Glu Val Ala Ala Ile Lys Ala Leu His Glu Ala Phe Pro
    210                 215                 220
Asp Ala Lys Leu Asp Leu Asp Pro Asn Gly Ala Trp Ser Leu Lys Glu
225                 230                 235                 240
Ala Leu His Tyr Ala Asp Glu Leu Lys Gly Val Leu His Tyr Ile Glu
                245                 250                 255
Asp Pro Cys Gly Gly Glu Asn Gly Phe Ser Gly Arg Glu Ile Leu Ser
            260                 265                 270
Glu Phe Gln Arg Ile Thr His Met Pro Thr Ala Thr Asn Met Val Asp
        275                 280                 285
Thr Asp Trp Arg Gln Met Ser His Ala Ile Ala Leu Asn Ala Val Ser
    290                 295                 300
Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Glu Gly Ala Val Arg
305                 310                 315                 320
Val Ala Gln Leu Cys His Glu Phe Asn Leu Asn Trp Gly Val His Ser
                325                 330                 335
Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Ala Asn Ala Ala Ala
            340                 345                 350
Ala Ala Pro Gly Asn Val Tyr Asp Val Asp Thr His Trp Ile Trp Gln
        355                 360                 365
Asp Gly Gln Asn Leu Thr Lys His Pro Tyr Glu Ile Lys Asn Gly Gln
    370                 375                 380
Leu Thr Val Pro Arg Thr Asn Glu Gly Leu Gly Val Glu Val Asp Met
385                 390                 395                 400
Asp Ala Ile Glu Arg Ala His Gln Leu Tyr Val Asp Asn Asn Leu Gly
                405                 410                 415
Ala Arg Asp Asp Ala Lys Ala Met Gln Tyr Leu Ile Pro Asn Trp Gln
            420                 425                 430
Phe Asp Ala Lys Arg Pro Ala Leu Val Arg
        435                 440

<210> 147
<211> 452
<212> PRT
<213> Th. carboxydivorans

<220> 
<223> glucarate dehydratase variant

<400> 147
Met Asn Asn Glu Thr Thr Lys Asn Gly Ile Ser Gly Ser Pro Val Val
 1               5                  10                  15
Thr Glu Leu His Val Ile Pro Val Ala Gly Arg Asp Ser Met Leu Leu
            20                  25                  30
Asn Leu Ser Gly Ala His Gly Pro Phe Phe Thr Arg Asn Ile Val Ile
        35                  40                  45
Leu Lys Asp Ser Ala Gly His Thr Gly Val Gly Glu Val Pro Gly Gly
    50                  55                  60
Glu Lys Ile Arg Gln Thr Ile Glu Asp Ala Arg Pro Leu Val Val Gly
65                  70                  75                  80
Gln Pro Ile Gly Lys Tyr Asn Asn Ile Leu Thr Thr Val Arg Gln Arg
                85                  90                  95
Phe Ala Asp Arg Asp Ala Gly Gly Arg Gly Leu Gln Thr Phe Asp Leu
            100                 105                 110
Arg Ile Thr Ile His Ala Val Thr Ala Leu Glu Ala Ala Leu Leu Asp
        115                 120                 125
Leu Leu Gly Gln Phe Leu Gly Val Pro Val Ala Ala Leu Leu Gly Glu
    130                 135                 140
Gly Gln Gln Arg Asp Glu Val Glu Met Leu Gly Tyr Leu Phe Tyr Ile
145                 150                 155                 160
Gly Asp Arg Lys Lys Thr Asp Leu Pro Tyr Tyr Ser Ala Pro Glu Ala
                165                 170                 175
Lys Asp Asn Trp Leu Arg Ile Arg His Glu Glu Ala Met Thr Pro Gln
            180                 185                 190
Ala Ile Val Glu Leu Ala Glu Ala Ala Tyr Glu Arg Tyr Gly Phe Asn
        195                 200                 205
Asp Phe Lys Leu Lys Gly Gly Val Leu Arg Gly Glu Glu Glu Met Glu
    210                 215                 220
Ala Val Ile Ala Leu Ala Lys Arg Phe Ser Lys Ala Arg Ile Thr Ile
225                 230                 235                 240
Asp Pro Asn Gly Ala Trp Ser Leu Glu Glu Ala Ile Lys Leu Cys Arg
                245                 250                 255
Gly Lys His Asp Val Leu Ala Tyr Ala Glu Asp Pro Cys Gly Ala Glu
            260                 265                 270
Asn Gly Tyr Ser Gly Arg Glu Ile Met Ala Glu Phe Arg Arg Ala Thr
        275                 280                 285
Gly Ile Pro Thr Ala Thr Asn Met Ile Ala Thr Asp Trp Arg Gln Met
    290                 295                 300
Gly His Ala Ile Gln Leu His Ser Ile Asp Ile Pro Leu Ala Asp Pro
305                 310                 315                 320
His Phe Trp Thr Met Gln Gly Ser Val Arg Val Ala Gln Met Cys His
                325                 330                 335
Glu Trp Gly Leu Thr Trp Gly Ser His Ser Asn Asn His Phe Asp Ile
            340                 345                 350
Ser Leu Ala Met Phe Thr His Val Gly Ala Ala Ala Pro Gly Lys Ile
        355                 360                 365
Thr Ala Leu Asp Thr His Trp Ile Trp Gln Glu Gly Ile Glu Arg Leu
    370                 375                 380
Thr Lys Glu Pro Leu Lys Ile Val Asn Gly Lys Val Thr Val Pro Asp
385                 390                 395                 400
Lys Pro Gly Leu Gly Ile Glu Val Asp Met Glu Gln Ile Glu Lys Ala
                405                 410                 415
His Gln Ile Tyr Lys Lys Leu Gly Leu Gly Ala Arg Asp Asp Thr Val
            420                 425                 430
Ala Met Gln Tyr Leu Ile Pro Gly Trp Lys Phe Asp Asn Lys Arg Pro
        435                 440                 445
Cys Leu Val Arg
    450

<210> 148
<211> 444
<212> PRT
<213> Streptococcus Sp. OK885

<220> 
<223> glucarate dehydratase variant

<400> 148
Met Ser Lys Ser Gln Pro Thr Val Thr Ala Phe Ser Val Tyr Pro Val
 1               5                  10                  15
Ala Gly Arg Asp Ser Met Glu Leu Asn Leu Ser Gly Ala His Gly Pro
            20                  25                  30
Tyr Phe Thr Arg Asn Val Val Val Leu Thr Asp Ser Glu Gly Arg Thr
        35                  40                  45
Gly Leu Gly Glu Val Pro Gly Gly Glu Lys Ile Thr Arg Thr Leu Arg
    50                  55                  60
Asp Ala Glu Ser Leu Val Val Gly Ala Lys Val Gly Asp Tyr Lys Arg
65                  70                  75                  80
Val Leu Arg Glu Ile Gly Asp Arg Phe Ala Asp Arg Asp Ala Gly Gly
                85                  90                  95
Arg Gly Ala Gln Thr Phe Asp Leu Arg Thr Thr Val His Ala Val Thr
            100                 105                 110
Ala Val Glu Ser Ala Leu Leu Asp Leu Leu Gly Gln His Leu Asp Val
        115                 120                 125
Pro Val Ala Ala Leu Leu Gly Asp Gly Gln Gln Arg Asp Ala Val Arg
    130                 135                 140
Val Leu Gly Tyr Leu Phe Tyr Val Gly Asp Pro Gly Arg Thr Asp Leu
145                 150                 155                 160
Glu Tyr Val Arg Glu Pro Asp Ala Ala Glu Asp Trp Tyr Arg Ile Arg
                165                 170                 175
His Glu Glu Ala Leu Ser Pro Glu Ala Ile Val Arg Gln Ala Glu Ala
            180                 185                 190
Thr His Asp Leu Tyr Gly Phe Arg Asp Phe Lys Leu Lys Gly Gly Val
        195                 200                 205
Leu Ala Gly Ala Glu Glu Val Lys Ala Val Arg Ala Leu Lys Asp Arg
    210                 215                 220
Phe Pro Glu Ala Arg Ile Thr Leu Asp Pro Asn Gly Ala Trp Ser Leu
225                 230                 235                 240
Arg Glu Ala Val Glu Leu Cys Thr Pro Leu Val Gly Thr Leu Ala Tyr
                245                 250                 255
Ala Glu Asp Pro Cys Gly Ala Glu Gly Gly Tyr Ser Gly Arg Glu Ile
            260                 265                 270
Leu Ala Glu Phe Arg Arg Ala Thr Gly Leu Pro Thr Ala Thr Asn Met
        275                 280                 285
Ile Ala Thr Asp Trp Arg Gln Leu Thr His Ala Leu Ala Leu Gln Ser
    290                 295                 300
Val Ser Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ser
305                 310                 315                 320
Val Arg Val Ala Gln Leu Cys Asn Ala Met Gly Leu Thr Trp Gly Cys
                325                 330                 335
His Ser Asn Asn His Phe Asp Ile Ser Leu Ala Met Val Thr His Cys
            340                 345                 350
Gly Ala Ala Ala Pro Gly Ala Tyr Asn Ala Leu Asp Thr His Trp Ile
        355                 360                 365
Trp Gln Glu Gly Leu Glu Arg Leu Thr Val Glu Pro Pro Arg Ile Val
    370                 375                 380
Asp Gly Glu Ile Ala Val Pro Asp Ala Pro Gly Leu Gly Val Arg Leu
385                 390                 395                 400
Asp Arg Glu Arg Leu Leu Ala Ala His Glu Leu Tyr Gln Glu Lys Ala
                405                 410                 415
Leu Gly Ala Arg Asp Asp Ala Val Gly Met Arg Tyr Leu Val Pro Gly
            420                 425                 430
Trp Glu Phe Asp Ser Lys Arg Pro Cys Leu Val Arg
        435                 440
