                         SEQUENCE LISTING

<110>  Novavax, Inc.
 
<120>  CORONAVIRUS VACCINE FORMULATIONS

<130>  NOVV-092/01WO 305047-2992

<150>  US 63/293,468
<151>  2021-12-23

<150>  US 63/292,196
<151>  2021-12-21

<150>  US 63/290,439
<151>  2021-12-16

<150>  US 63/280,395
<151>  2021-11-17

<150>  US 63/195,986
<151>  2021-06-02

<150>  US 63/177,059
<151>  2021-04-20

<150>  US 63/176,825
<151>  2021-04-19

<150>  US 63/164,487
<151>  2021-03-22

<160>  168   

<170>  PatentIn version 3.5

<210>  1
<211>  1273
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  2
<211>  1260
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  2

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  3
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT S PROTEIN

<400>  3

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  4
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT S PROTEIN

<400>  4

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  5
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIGNAL PEPTIDE

<400>  5

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser 
1               5                   10              


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  6

Arg Arg Ala Arg 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  7

Gln Gln Ala Gln 
1               


<210>  8
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  8

Gln Arg Ala Arg 
1               


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  9

Arg Gln Ala Arg 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  10

Arg Arg Ala Gln 
1               


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  11

Gln Gln Ala Arg 
1               


<210>  12
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  12

Arg Gln Ala Gln 
1               


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  13

Gln Arg Ala Gln 
1               


<210>  14
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  14

Asn Asn Ala Asn 
1               


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  15

Asn Arg Ala Arg 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  16

Arg Asn Ala Arg 
1               


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  17

Arg Arg Ala Asn 
1               


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  18

Asn Asn Ala Arg 
1               


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  19

Arg Asn Ala Asn 
1               


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  20

Asn Arg Ala Asn 
1               


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  21

Ala Ala Ala Ala 
1               


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  22

Ala Arg Ala Arg 
1               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  23

Arg Ala Ala Arg 
1               


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  24

Arg Arg Ala Ala 
1               


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  25

Ala Ala Ala Arg 
1               


<210>  26
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  26

Arg Ala Ala Ala 
1               


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  27

Ala Arg Ala Ala 
1               


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  28

Gly Gly Ala Gly 
1               


<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  29

Gly Arg Ala Arg 
1               


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  30

Arg Gly Ala Arg 
1               


<210>  31
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  31

Arg Arg Ala Gly 
1               


<210>  32
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  32

Gly Gly Ala Arg 
1               


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  33

Arg Gly Ala Gly 
1               


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT FURIN CLEAVAGE SITE

<400>  34

Gly Arg Ala Gly 
1               


<210>  35
<211>  3819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  35
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacgcaggg ctcgctctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctt ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tctgaacccg tcctgaaggg cgtgaagctg cactacacc                            3819


<210>  36
<211>  1279
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  36

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr His His  His His His 
    1265                 1270                 1275             


His 
    


<210>  37
<211>  3837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  37
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacgcaggg ctcgctctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctt ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tctgaacccg tcctgaaggg cgtgaagctg cactacaccc accaccacca ccaccac        3837


<210>  38
<211>  1266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  38

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  His His His 
    1250                 1255                 1260             


His His  His 
    1265     


<210>  39
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  39

Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met 
1               5                   10                  15      


Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys 
            20                  25                  30          


Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser 
        35                  40                  45              


Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr 
    50                  55                  60  


<210>  40
<211>  1213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  40

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1205                 1210             


<210>  41
<211>  1200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  41

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  
    1190                 1195                 1200 


<210>  42
<211>  1219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  42

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro His His  His His His 
    1205                 1210                 1215             


His 
    


<210>  43
<211>  3657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  43
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacgcaggg ctcgctctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctc accaccacca ccaccac        3657


<210>  44
<211>  1219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  44

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro His His  His His His 
    1205                 1210                 1215             


His 
    


<210>  45
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  45

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
305                 310                 315             


<210>  46
<211>  955
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  46

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
    210                 215                 220                 


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
225                 230                 235                 240 


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
                245                 250                 255     


Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
            260                 265                 270         


Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
        275                 280                 285             


Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
    290                 295                 300                 


Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
305                 310                 315                 320 


Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
                325                 330                 335     


His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
            340                 345                 350         


Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser 
        355                 360                 365             


Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu 
    370                 375                 380                 


Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe 
385                 390                 395                 400 


Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr 
                405                 410                 415     


Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
            420                 425                 430         


Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala 
        435                 440                 445             


Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe 
    450                 455                 460                 


Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly 
465                 470                 475                 480 


Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
                485                 490                 495     


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
            500                 505                 510         


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
        515                 520                 525             


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
    530                 535                 540                 


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
545                 550                 555                 560 


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
                565                 570                 575     


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
            580                 585                 590         


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
        595                 600                 605             


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
    610                 615                 620                 


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
625                 630                 635                 640 


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
                645                 650                 655     


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
            660                 665                 670         


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
        675                 680                 685             


Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala 
    690                 695                 700                 


Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys 
705                 710                 715                 720 


Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln 
                725                 730                 735     


Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala 
            740                 745                 750         


Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys 
        755                 760                 765             


Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp 
    770                 775                 780                 


Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp 
785                 790                 795                 800 


Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn 
                805                 810                 815     


Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu 
            820                 825                 830         


Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu 
        835                 840                 845             


Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu 
    850                 855                 860                 


Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile 
865                 870                 875                 880 


Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp 
                885                 890                 895     


Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
            900                 905                 910         


Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly 
        915                 920                 925             


Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu 
    930                 935                 940                 


Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr 
945                 950                 955 


<210>  47
<211>  2868
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  47
atgttcgtct tcctggtgct gctgcccctg gtgtccagca tcactaacct gtgccccttc       60

ggagaggtct tcaacgccac ccgcttcgct tccgtgtacg cctggaacag gaagagaatc      120

tcaaactgcg tcgctgacta ctccgtgctg tacaactcag cctccttcag caccttcaag      180

tgctacggcg tgtcaccaac taagctgaac gacctgtgct tcaccaacgt ctacgccgac      240

tccttcgtga tcaggggaga cgaggtcaga cagatcgctc ctggccagac tggaaagatc      300

gccgactaca actacaagct gcccgacgac ttcaccggtt gcgtcatcgc ttggaacagc      360

aacaacctgg actctaaagt gggtggcaac tacaactacc tgtaccgcct gttccgtaag      420

tcaaacctga agccattcga gagggacatc agcactgaaa tctaccaggc cggatctacc      480

ccttgcaacg gtgtcgaggg cttcaactgc tacttccccc tgcagtccta cggtttccag      540

ccaactaacg gtgtgggcta ccagccttac agagtggtcg tgctgagctt cgaactgctc      600

cacgctcctg ctactgtgtg cggtccaaag aagtctacca acctggtcaa gaacaagtgc      660

gtgaacttca acttcaacgg cctgaccgga actggtgtcc tgaccgagag caacaagaag      720

ttcctgccct tccagcagtt cggaagggac atcgctgaca ccactgacgc tgtgcgcgac      780

cctcagaccc tggaaatcct ggacatcact ccatgctcat tcggaggtgt ctccgtgatc      840

acccctggca ccaacacttc taaccaggtc gctgtgctgt accaggacgt caactgcacc      900

gaggtccctg tggccatcca cgctgaccag ctgaccccca cttggcgcgt gtactccacc      960

ggcagcaacg tgttccagac tcgtgctggt tgcctgatcg gcgccgagca cgtgaacaac     1020

agctacgaat gcgacatccc catcggcgct ggaatctgcg cctcttacca gacccagact     1080

aacagcccac gcagggctcg ctctgtggcc tctcagtcaa tcatcgctta caccatgtca     1140

ctgggcgctg aaaactccgt ggcctactct aacaactcaa tcgccatccc caccaacttc     1200

actatcagcg tgaccactga gatcctgcca gtcagcatga ccaagacttc tgtggactgc     1260

actatgtaca tctgcggaga cagcaccgaa tgctctaacc tgctgctgca gtacggctct     1320

ttctgcaccc agctgaaccg tgctctgact ggaatcgccg tggagcagga caagaacact     1380

caggaagtct tcgctcaggt gaagcaaatc tacaagaccc cacctatcaa ggacttcggc     1440

ggattcaact tctcccagat cctgcctgac ccctccaagc caagcaagcg ctctttcatc     1500

gaggacctgc tgttcaacaa ggtcactctg gccgacgctg gattcatcaa gcagtacgga     1560

gactgcctgg gtgacatcgc cgctcgtgac ctgatctgcg ctcagaagtt caacggtctg     1620

accgtgctgc ccccactgct gactgacgaa atgatcgccc agtacactag cgccctgctg     1680

gctggaacca tcacttctgg ttggaccttc ggtgctggcg ccgctctgca gatccctttc     1740

gctatgcaga tggcctaccg tttcaacgga atcggtgtca cccagaacgt gctgtacgag     1800

aaccagaagc tgatcgctaa ccagttcaac tcagccatcg gaaagatcca ggacagcctg     1860

agctctactg cctctgctct gggcaagctg caggacgtcg tgaaccagaa cgcccaggct     1920

ctgaacaccc tggtcaagca gctgtcatcc aacttcggtg ctatcagctc tgtgctgaac     1980

gacatcctgt cccgcctgga caaggtcgag gccgaagtgc agatcgaccg cctgatcact     2040

ggccgtctgc agtcactgca gacctacgtg actcagcagc tgatcagggc cgctgaaatc     2100

agagcctccg ctaacctggc cgctaccaag atgagcgagt gcgtcctggg tcaatctaag     2160

cgtgtggact tctgcggcaa gggataccac ctgatgtcat tccctcagtc tgctccccac     2220

ggtgtggtgt tcctgcacgt cacctacgtg ccagcccagg agaagaactt caccactgcc     2280

cctgctatct gccacgacgg caaggctcac ttccccaggg aaggtgtctt cgtgagcaac     2340

ggcacccact ggttcgtcac tcagagaaac ttctacgagc cacagatcat caccactgac     2400

aacactttcg tgtctggaaa ctgcgacgtg gtcatcggta tcgtcaacaa caccgtgtac     2460

gaccccctgc agccagagct ggactcattc aaggaggaac tggacaagta cttcaagaac     2520

cacacctccc ctgacgtcga cctgggcgac atctcaggaa tcaacgcttc cgtcgtgaac     2580

atccagaagg agatcgaccg cctgaacgaa gtggccaaga acctgaacga aagcctgatc     2640

gacctgcagg agctgggcaa gtacgaacag tacatcaagt ggccttggta catctggctg     2700

ggtttcatcg ctggcctcat cgctatcgtg atggtgacca tcatgctgtg ctgcatgact     2760

tcatgctgct cctgcctgaa gggctgctgc agctgcggat cttgctgcaa gttcgacgag     2820

gacgactctg aacccgtcct gaagggcgtg aagctgcact acacctaa                  2868


<210>  48
<211>  942
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  48

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
1               5                   10                  15      


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
            20                  25                  30          


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
        35                  40                  45              


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
    50                  55                  60                  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
65                  70                  75                  80  


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
                85                  90                  95      


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
            100                 105                 110         


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
        115                 120                 125             


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
    130                 135                 140                 


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
145                 150                 155                 160 


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
                165                 170                 175     


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
            180                 185                 190         


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
        195                 200                 205             


Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser 
    210                 215                 220                 


Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp 
225                 230                 235                 240 


Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile 
                245                 250                 255     


Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn 
            260                 265                 270         


Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu 
        275                 280                 285             


Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val 
    290                 295                 300                 


Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile 
305                 310                 315                 320 


Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly 
                325                 330                 335     


Ala Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg 
            340                 345                 350         


Ala Arg Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu 
        355                 360                 365             


Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro 
    370                 375                 380                 


Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met 
385                 390                 395                 400 


Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr 
                405                 410                 415     


Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu 
            420                 425                 430         


Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln 
        435                 440                 445             


Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
    450                 455                 460                 


Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys 
465                 470                 475                 480 


Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr 
                485                 490                 495     


Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp 
            500                 505                 510         


Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr 
        515                 520                 525             


Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser 
    530                 535                 540                 


Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly 
545                 550                 555                 560 


Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn 
                565                 570                 575     


Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile 
            580                 585                 590         


Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser 
        595                 600                 605             


Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn 
    610                 615                 620                 


Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly 
625                 630                 635                 640 


Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val 
                645                 650                 655     


Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser 
            660                 665                 670         


Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg 
        675                 680                 685             


Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
    690                 695                 700                 


Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser 
705                 710                 715                 720 


Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr 
                725                 730                 735     


Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His 
            740                 745                 750         


Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly 
        755                 760                 765             


Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile 
    770                 775                 780                 


Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly 
785                 790                 795                 800 


Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser 
                805                 810                 815     


Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp 
            820                 825                 830         


Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile 
        835                 840                 845             


Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu 
    850                 855                 860                 


Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys 
865                 870                 875                 880 


Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile 
                885                 890                 895     


Val Met Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys 
            900                 905                 910         


Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp 
        915                 920                 925             


Asp Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr 
    930                 935                 940         


<210>  49
<211>  960
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  49

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
    210                 215                 220                 


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
225                 230                 235                 240 


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
                245                 250                 255     


Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
            260                 265                 270         


Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
        275                 280                 285             


Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
    290                 295                 300                 


Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
305                 310                 315                 320 


Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
                325                 330                 335     


His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
            340                 345                 350         


Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser 
        355                 360                 365             


Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu 
    370                 375                 380                 


Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe 
385                 390                 395                 400 


Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr 
                405                 410                 415     


Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
            420                 425                 430         


Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala 
        435                 440                 445             


Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe 
    450                 455                 460                 


Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly 
465                 470                 475                 480 


Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
                485                 490                 495     


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
            500                 505                 510         


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
        515                 520                 525             


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
    530                 535                 540                 


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
545                 550                 555                 560 


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
                565                 570                 575     


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
            580                 585                 590         


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
        595                 600                 605             


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
    610                 615                 620                 


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
625                 630                 635                 640 


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
                645                 650                 655     


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
            660                 665                 670         


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
        675                 680                 685             


Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala 
    690                 695                 700                 


Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys 
705                 710                 715                 720 


Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln 
                725                 730                 735     


Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala 
            740                 745                 750         


Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys 
        755                 760                 765             


Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp 
    770                 775                 780                 


Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp 
785                 790                 795                 800 


Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn 
                805                 810                 815     


Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu 
            820                 825                 830         


Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu 
        835                 840                 845             


Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu 
    850                 855                 860                 


Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile 
865                 870                 875                 880 


Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp 
                885                 890                 895     


Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
            900                 905                 910         


Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly 
        915                 920                 925             


Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu 
    930                 935                 940                 


Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr His His His His His 
945                 950                 955                 960 


<210>  50
<211>  2883
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  50
atgttcgtct tcctggtgct gctgcccctg gtgtccagca tcactaacct gtgccccttc       60

ggagaggtct tcaacgccac ccgcttcgct tccgtgtacg cctggaacag gaagagaatc      120

tcaaactgcg tcgctgacta ctccgtgctg tacaactcag cctccttcag caccttcaag      180

tgctacggcg tgtcaccaac taagctgaac gacctgtgct tcaccaacgt ctacgccgac      240

tccttcgtga tcaggggaga cgaggtcaga cagatcgctc ctggccagac tggaaagatc      300

gccgactaca actacaagct gcccgacgac ttcaccggtt gcgtcatcgc ttggaacagc      360

aacaacctgg actctaaagt gggtggcaac tacaactacc tgtaccgcct gttccgtaag      420

tcaaacctga agccattcga gagggacatc agcactgaaa tctaccaggc cggatctacc      480

ccttgcaacg gtgtcgaggg cttcaactgc tacttccccc tgcagtccta cggtttccag      540

ccaactaacg gtgtgggcta ccagccttac agagtggtcg tgctgagctt cgaactgctc      600

cacgctcctg ctactgtgtg cggtccaaag aagtctacca acctggtcaa gaacaagtgc      660

gtgaacttca acttcaacgg cctgaccgga actggtgtcc tgaccgagag caacaagaag      720

ttcctgccct tccagcagtt cggaagggac atcgctgaca ccactgacgc tgtgcgcgac      780

cctcagaccc tggaaatcct ggacatcact ccatgctcat tcggaggtgt ctccgtgatc      840

acccctggca ccaacacttc taaccaggtc gctgtgctgt accaggacgt caactgcacc      900

gaggtccctg tggccatcca cgctgaccag ctgaccccca cttggcgcgt gtactccacc      960

ggcagcaacg tgttccagac tcgtgctggt tgcctgatcg gcgccgagca cgtgaacaac     1020

agctacgaat gcgacatccc catcggcgct ggaatctgcg cctcttacca gacccagact     1080

aacagcccac gcagggctcg ctctgtggcc tctcagtcaa tcatcgctta caccatgtca     1140

ctgggcgctg aaaactccgt ggcctactct aacaactcaa tcgccatccc caccaacttc     1200

actatcagcg tgaccactga gatcctgcca gtcagcatga ccaagacttc tgtggactgc     1260

actatgtaca tctgcggaga cagcaccgaa tgctctaacc tgctgctgca gtacggctct     1320

ttctgcaccc agctgaaccg tgctctgact ggaatcgccg tggagcagga caagaacact     1380

caggaagtct tcgctcaggt gaagcaaatc tacaagaccc cacctatcaa ggacttcggc     1440

ggattcaact tctcccagat cctgcctgac ccctccaagc caagcaagcg ctctttcatc     1500

gaggacctgc tgttcaacaa ggtcactctg gccgacgctg gattcatcaa gcagtacgga     1560

gactgcctgg gtgacatcgc cgctcgtgac ctgatctgcg ctcagaagtt caacggtctg     1620

accgtgctgc ccccactgct gactgacgaa atgatcgccc agtacactag cgccctgctg     1680

gctggaacca tcacttctgg ttggaccttc ggtgctggcg ccgctctgca gatccctttc     1740

gctatgcaga tggcctaccg tttcaacgga atcggtgtca cccagaacgt gctgtacgag     1800

aaccagaagc tgatcgctaa ccagttcaac tcagccatcg gaaagatcca ggacagcctg     1860

agctctactg cctctgctct gggcaagctg caggacgtcg tgaaccagaa cgcccaggct     1920

ctgaacaccc tggtcaagca gctgtcatcc aacttcggtg ctatcagctc tgtgctgaac     1980

gacatcctgt cccgcctgga caaggtcgag gccgaagtgc agatcgaccg cctgatcact     2040

ggccgtctgc agtcactgca gacctacgtg actcagcagc tgatcagggc cgctgaaatc     2100

agagcctccg ctaacctggc cgctaccaag atgagcgagt gcgtcctggg tcaatctaag     2160

cgtgtggact tctgcggcaa gggataccac ctgatgtcat tccctcagtc tgctccccac     2220

ggtgtggtgt tcctgcacgt cacctacgtg ccagcccagg agaagaactt caccactgcc     2280

cctgctatct gccacgacgg caaggctcac ttccccaggg aaggtgtctt cgtgagcaac     2340

ggcacccact ggttcgtcac tcagagaaac ttctacgagc cacagatcat caccactgac     2400

aacactttcg tgtctggaaa ctgcgacgtg gtcatcggta tcgtcaacaa caccgtgtac     2460

gaccccctgc agccagagct ggactcattc aaggaggaac tggacaagta cttcaagaac     2520

cacacctccc ctgacgtcga cctgggcgac atctcaggaa tcaacgcttc cgtcgtgaac     2580

atccagaagg agatcgaccg cctgaacgaa gtggccaaga acctgaacga aagcctgatc     2640

gacctgcagg agctgggcaa gtacgaacag tacatcaagt ggccttggta catctggctg     2700

ggtttcatcg ctggcctcat cgctatcgtg atggtgacca tcatgctgtg ctgcatgact     2760

tcatgctgct cctgcctgaa gggctgctgc agctgcggat cttgctgcaa gttcgacgag     2820

gacgactctg aacccgtcct gaagggcgtg aagctgcact acacccacca ccaccaccac     2880

cac                                                                   2883


<210>  51
<211>  947
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  51

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
1               5                   10                  15      


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
            20                  25                  30          


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
        35                  40                  45              


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
    50                  55                  60                  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
65                  70                  75                  80  


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
                85                  90                  95      


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
            100                 105                 110         


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
        115                 120                 125             


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
    130                 135                 140                 


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
145                 150                 155                 160 


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
                165                 170                 175     


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
            180                 185                 190         


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
        195                 200                 205             


Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser 
    210                 215                 220                 


Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp 
225                 230                 235                 240 


Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile 
                245                 250                 255     


Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn 
            260                 265                 270         


Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu 
        275                 280                 285             


Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val 
    290                 295                 300                 


Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile 
305                 310                 315                 320 


Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly 
                325                 330                 335     


Ala Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg 
            340                 345                 350         


Ala Arg Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu 
        355                 360                 365             


Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro 
    370                 375                 380                 


Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met 
385                 390                 395                 400 


Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr 
                405                 410                 415     


Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu 
            420                 425                 430         


Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln 
        435                 440                 445             


Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
    450                 455                 460                 


Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys 
465                 470                 475                 480 


Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr 
                485                 490                 495     


Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp 
            500                 505                 510         


Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr 
        515                 520                 525             


Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser 
    530                 535                 540                 


Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly 
545                 550                 555                 560 


Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn 
                565                 570                 575     


Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile 
            580                 585                 590         


Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser 
        595                 600                 605             


Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn 
    610                 615                 620                 


Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly 
625                 630                 635                 640 


Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val 
                645                 650                 655     


Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser 
            660                 665                 670         


Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg 
        675                 680                 685             


Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
    690                 695                 700                 


Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser 
705                 710                 715                 720 


Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr 
                725                 730                 735     


Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His 
            740                 745                 750         


Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly 
        755                 760                 765             


Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile 
    770                 775                 780                 


Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly 
785                 790                 795                 800 


Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser 
                805                 810                 815     


Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp 
            820                 825                 830         


Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile 
        835                 840                 845             


Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu 
    850                 855                 860                 


Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys 
865                 870                 875                 880 


Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile 
                885                 890                 895     


Val Met Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys 
            900                 905                 910         


Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp 
        915                 920                 925             


Asp Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr His His 
    930                 935                 940                 


His His His 
945         


<210>  52
<211>  901
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  52

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
    210                 215                 220                 


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
225                 230                 235                 240 


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
                245                 250                 255     


Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
            260                 265                 270         


Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
        275                 280                 285             


Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
    290                 295                 300                 


Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
305                 310                 315                 320 


Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
                325                 330                 335     


His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
            340                 345                 350         


Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser 
        355                 360                 365             


Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu 
    370                 375                 380                 


Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe 
385                 390                 395                 400 


Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr 
                405                 410                 415     


Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
            420                 425                 430         


Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala 
        435                 440                 445             


Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe 
    450                 455                 460                 


Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly 
465                 470                 475                 480 


Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
                485                 490                 495     


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
            500                 505                 510         


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
        515                 520                 525             


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
    530                 535                 540                 


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
545                 550                 555                 560 


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
                565                 570                 575     


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
            580                 585                 590         


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
        595                 600                 605             


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
    610                 615                 620                 


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
625                 630                 635                 640 


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
                645                 650                 655     


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
            660                 665                 670         


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
        675                 680                 685             


Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala 
    690                 695                 700                 


Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys 
705                 710                 715                 720 


Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln 
                725                 730                 735     


Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala 
            740                 745                 750         


Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys 
        755                 760                 765             


Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp 
    770                 775                 780                 


Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp 
785                 790                 795                 800 


Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn 
                805                 810                 815     


Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu 
            820                 825                 830         


Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu 
        835                 840                 845             


Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu 
    850                 855                 860                 


Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile 
865                 870                 875                 880 


Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro His 
                885                 890                 895     


His His His His His 
            900     


<210>  53
<211>  2706
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  53
atgttcgtct tcctggtgct gctgcccctg gtgtccagca tcactaacct gtgccccttc       60

ggagaggtct tcaacgccac ccgcttcgct tccgtgtacg cctggaacag gaagagaatc      120

tcaaactgcg tcgctgacta ctccgtgctg tacaactcag cctccttcag caccttcaag      180

tgctacggcg tgtcaccaac taagctgaac gacctgtgct tcaccaacgt ctacgccgac      240

tccttcgtga tcaggggaga cgaggtcaga cagatcgctc ctggccagac tggaaagatc      300

gccgactaca actacaagct gcccgacgac ttcaccggtt gcgtcatcgc ttggaacagc      360

aacaacctgg actctaaagt gggtggcaac tacaactacc tgtaccgcct gttccgtaag      420

tcaaacctga agccattcga gagggacatc agcactgaaa tctaccaggc cggatctacc      480

ccttgcaacg gtgtcgaggg cttcaactgc tacttccccc tgcagtccta cggtttccag      540

ccaactaacg gtgtgggcta ccagccttac agagtggtcg tgctgagctt cgaactgctc      600

cacgctcctg ctactgtgtg cggtccaaag aagtctacca acctggtcaa gaacaagtgc      660

gtgaacttca acttcaacgg cctgaccgga actggtgtcc tgaccgagag caacaagaag      720

ttcctgccct tccagcagtt cggaagggac atcgctgaca ccactgacgc tgtgcgcgac      780

cctcagaccc tggaaatcct ggacatcact ccatgctcat tcggaggtgt ctccgtgatc      840

acccctggca ccaacacttc taaccaggtc gctgtgctgt accaggacgt caactgcacc      900

gaggtccctg tggccatcca cgctgaccag ctgaccccca cttggcgcgt gtactccacc      960

ggcagcaacg tgttccagac tcgtgctggt tgcctgatcg gcgccgagca cgtgaacaac     1020

agctacgaat gcgacatccc catcggcgct ggaatctgcg cctcttacca gacccagact     1080

aacagcccac gcagggctcg ctctgtggcc tctcagtcaa tcatcgctta caccatgtca     1140

ctgggcgctg aaaactccgt ggcctactct aacaactcaa tcgccatccc caccaacttc     1200

actatcagcg tgaccactga gatcctgcca gtcagcatga ccaagacttc tgtggactgc     1260

actatgtaca tctgcggaga cagcaccgaa tgctctaacc tgctgctgca gtacggctct     1320

ttctgcaccc agctgaaccg tgctctgact ggaatcgccg tggagcagga caagaacact     1380

caggaagtct tcgctcaggt gaagcaaatc tacaagaccc cacctatcaa ggacttcggc     1440

ggattcaact tctcccagat cctgcctgac ccctccaagc caagcaagcg ctctttcatc     1500

gaggacctgc tgttcaacaa ggtcactctg gccgacgctg gattcatcaa gcagtacgga     1560

gactgcctgg gtgacatcgc cgctcgtgac ctgatctgcg ctcagaagtt caacggtctg     1620

accgtgctgc ccccactgct gactgacgaa atgatcgccc agtacactag cgccctgctg     1680

gctggaacca tcacttctgg ttggaccttc ggtgctggcg ccgctctgca gatccctttc     1740

gctatgcaga tggcctaccg tttcaacgga atcggtgtca cccagaacgt gctgtacgag     1800

aaccagaagc tgatcgctaa ccagttcaac tcagccatcg gaaagatcca ggacagcctg     1860

agctctactg cctctgctct gggcaagctg caggacgtcg tgaaccagaa cgcccaggct     1920

ctgaacaccc tggtcaagca gctgtcatcc aacttcggtg ctatcagctc tgtgctgaac     1980

gacatcctgt cccgcctgga caaggtcgag gccgaagtgc agatcgaccg cctgatcact     2040

ggccgtctgc agtcactgca gacctacgtg actcagcagc tgatcagggc cgctgaaatc     2100

agagcctccg ctaacctggc cgctaccaag atgagcgagt gcgtcctggg tcaatctaag     2160

cgtgtggact tctgcggcaa gggataccac ctgatgtcat tccctcagtc tgctccccac     2220

ggtgtggtgt tcctgcacgt cacctacgtg ccagcccagg agaagaactt caccactgcc     2280

cctgctatct gccacgacgg caaggctcac ttccccaggg aaggtgtctt cgtgagcaac     2340

ggcacccact ggttcgtcac tcagagaaac ttctacgagc cacagatcat caccactgac     2400

aacactttcg tgtctggaaa ctgcgacgtg gtcatcggta tcgtcaacaa caccgtgtac     2460

gaccccctgc agccagagct ggactcattc aaggaggaac tggacaagta cttcaagaac     2520

cacacctccc ctgacgtcga cctgggcgac atctcaggaa tcaacgcttc cgtcgtgaac     2580

atccagaagg agatcgaccg cctgaacgaa gtggccaaga acctgaacga aagcctgatc     2640

gacctgcagg agctgggcaa gtacgaacag tacatcaagt ggcctcacca ccaccaccac     2700

caccac                                                                2706


<210>  54
<211>  888
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  54

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
1               5                   10                  15      


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
            20                  25                  30          


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
        35                  40                  45              


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
    50                  55                  60                  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
65                  70                  75                  80  


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
                85                  90                  95      


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
            100                 105                 110         


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
        115                 120                 125             


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
    130                 135                 140                 


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
145                 150                 155                 160 


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
                165                 170                 175     


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
            180                 185                 190         


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
        195                 200                 205             


Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser 
    210                 215                 220                 


Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp 
225                 230                 235                 240 


Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile 
                245                 250                 255     


Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn 
            260                 265                 270         


Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu 
        275                 280                 285             


Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val 
    290                 295                 300                 


Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile 
305                 310                 315                 320 


Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly 
                325                 330                 335     


Ala Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg 
            340                 345                 350         


Ala Arg Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu 
        355                 360                 365             


Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro 
    370                 375                 380                 


Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met 
385                 390                 395                 400 


Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr 
                405                 410                 415     


Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu 
            420                 425                 430         


Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln 
        435                 440                 445             


Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
    450                 455                 460                 


Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys 
465                 470                 475                 480 


Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr 
                485                 490                 495     


Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp 
            500                 505                 510         


Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr 
        515                 520                 525             


Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser 
    530                 535                 540                 


Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly 
545                 550                 555                 560 


Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn 
                565                 570                 575     


Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile 
            580                 585                 590         


Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser 
        595                 600                 605             


Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn 
    610                 615                 620                 


Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly 
625                 630                 635                 640 


Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val 
                645                 650                 655     


Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser 
            660                 665                 670         


Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg 
        675                 680                 685             


Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
    690                 695                 700                 


Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser 
705                 710                 715                 720 


Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr 
                725                 730                 735     


Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His 
            740                 745                 750         


Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly 
        755                 760                 765             


Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile 
    770                 775                 780                 


Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly 
785                 790                 795                 800 


Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser 
                805                 810                 815     


Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp 
            820                 825                 830         


Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile 
        835                 840                 845             


Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu 
    850                 855                 860                 


Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys 
865                 870                 875                 880 


Trp Pro His His His His His His 
                885             


<210>  55
<211>  3819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  55
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacagcagg ctcagtctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctt ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tctgaacccg tcctgaaggg cgtgaagctg cactacacc                            3819


<210>  56
<211>  1279
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  56

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr His His  His His His 
    1265                 1270                 1275             


His 
    


<210>  57
<211>  3837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  57
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacagcagg ctcagtctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctt ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tctgaacccg tcctgaaggg cgtgaagctg cactacaccc accaccacca ccaccac        3837


<210>  58
<211>  1266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  58

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  His His His 
    1250                 1255                 1260             


His His  His 
    1265     


<210>  59
<211>  1219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  59

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro His His  His His His 
    1205                 1210                 1215             


His 
    


<210>  60
<211>  3657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  60
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacagcagg ctcagtctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctc accaccacca ccaccac        3657


<210>  61
<211>  1205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  61

Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
1               5                   10                  15      


Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser 
            20                  25                  30          


Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val 
        35                  40                  45              


Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg 
    50                  55                  60                  


Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser 
65                  70                  75                  80  


Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu 
                85                  90                  95      


Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val 
            100                 105                 110         


Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly 
        115                 120                 125             


Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg 
    130                 135                 140                 


Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro 
145                 150                 155                 160 


Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg 
                165                 170                 175     


Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys 
            180                 185                 190         


His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala 
        195                 200                 205             


Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe 
    210                 215                 220                 


Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser 
225                 230                 235                 240 


Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
                245                 250                 255     


Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr 
            260                 265                 270         


Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr 
        275                 280                 285             


Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe 
    290                 295                 300                 


Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
305                 310                 315                 320 


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
                325                 330                 335     


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
            340                 345                 350         


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
        355                 360                 365             


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
    370                 375                 380                 


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
385                 390                 395                 400 


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
                405                 410                 415     


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
            420                 425                 430         


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        435                 440                 445             


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    450                 455                 460                 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
465                 470                 475                 480 


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
        515                 520                 525             


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
    530                 535                 540                 


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
545                 550                 555                 560 


Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
                565                 570                 575     


Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
            580                 585                 590         


Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
        595                 600                 605             


Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
    610                 615                 620                 


Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 


His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     


Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser 
            660                 665                 670         


Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu 
        675                 680                 685             


Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe 
    690                 695                 700                 


Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr 
705                 710                 715                 720 


Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
                725                 730                 735     


Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala 
            740                 745                 750         


Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe 
        755                 760                 765             


Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly 
    770                 775                 780                 


Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
785                 790                 795                 800 


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
                805                 810                 815     


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
            820                 825                 830         


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
        835                 840                 845             


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
    850                 855                 860                 


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
865                 870                 875                 880 


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
                885                 890                 895     


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
            900                 905                 910         


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
        915                 920                 925             


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
    930                 935                 940                 


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
945                 950                 955                 960 


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
                965                 970                 975     


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
            980                 985                 990         


Tyr Val Thr Gln Gln Leu Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala 
        995                 1000                 1005             


Asn Leu  Ala Ala Thr Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser 
    1010                 1015                 1020             


Lys Arg  Val Asp Phe Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe 
    1025                 1030                 1035             


Pro Gln  Ser Ala Pro His Gly  Val Val Phe Leu His  Val Thr Tyr 
    1040                 1045                 1050             


Val Pro  Ala Gln Glu Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys 
    1055                 1060                 1065             


His Asp  Gly Lys Ala His Phe  Pro Arg Glu Gly Val  Phe Val Ser 
    1070                 1075                 1080             


Asn Gly  Thr His Trp Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro 
    1085                 1090                 1095             


Gln Ile  Ile Thr Thr Asp Asn  Thr Phe Val Ser Gly  Asn Cys Asp 
    1100                 1105                 1110             


Val Val  Ile Gly Ile Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln 
    1115                 1120                 1125             


Pro Glu  Leu Asp Ser Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys 
    1130                 1135                 1140             


Asn His  Thr Ser Pro Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile 
    1145                 1150                 1155             


Asn Ala  Ser Val Val Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn 
    1160                 1165                 1170             


Glu Val  Ala Lys Asn Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu 
    1175                 1180                 1185             


Leu Gly  Lys Tyr Glu Gln Tyr  Ile Lys Trp Pro His  His His His 
    1190                 1195                 1200             


His His  
    1205 


<210>  62
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  62

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile 
    690                 695                 700                 


Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser 
705                 710                 715                 720 


Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser 
                725                 730                 735     


Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln 
            740                 745                 750         


Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr 
        755                 760                 765             


Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile 
    770                 775                 780                 


Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser 
785                 790                 795                 800 


Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val 
                805                 810                 815     


Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly 
            820                 825                 830         


Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu 
        835                 840                 845             


Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr 
    850                 855                 860                 


Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala 
865                 870                 875                 880 


Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe 
                885                 890                 895     


Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu 
            900                 905                 910         


Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu 
        915                 920                 925             


Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln 
    930                 935                 940                 


Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe 
945                 950                 955                 960 


Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys 
                965                 970                 975     


Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln 
            980                 985                 990         


Ser Leu Gln Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
        995                 1000                 1005             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1010                 1015                 1020             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1025                 1030                 1035             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1040                 1045                 1050             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1055                 1060                 1065             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1070                 1075                 1080             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1085                 1090                 1095             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1100                 1105                 1110             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1115                 1120                 1125             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1130                 1135                 1140             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1145                 1150                 1155             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1160                 1165                 1170             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1175                 1180                 1185             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1190                 1195                 1200             


Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val 
    1205                 1210                 1215             


Met Val  Thr Ile Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys 
    1220                 1225                 1230             


Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu 
    1235                 1240                 1245             


Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255                 1260             


<210>  63
<211>  1250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  63

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser 
        675                 680                 685             


Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu 
    690                 695                 700                 


Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys 
705                 710                 715                 720 


Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe 
                725                 730                 735     


Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp 
            740                 745                 750         


Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr 
        755                 760                 765             


Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro 
    770                 775                 780                 


Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe 
785                 790                 795                 800 


Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp 
                805                 810                 815     


Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
            820                 825                 830         


Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala 
        835                 840                 845             


Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr 
    850                 855                 860                 


Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
865                 870                 875                 880 


Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn 
                885                 890                 895     


Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln 
            900                 905                 910         


Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val 
        915                 920                 925             


Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser 
    930                 935                 940                 


Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg 
945                 950                 955                 960 


Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly 
                965                 970                 975     


Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala 
            980                 985                 990         


Ala Glu Ile Arg Ala Ser Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu 
        995                 1000                 1005             


Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly 
    1010                 1015                 1020             


Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala Pro His  Gly Val Val 
    1025                 1030                 1035             


Phe Leu  His Val Thr Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr 
    1040                 1045                 1050             


Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys Ala His  Phe Pro Arg 
    1055                 1060                 1065             


Glu Gly  Val Phe Val Ser Asn  Gly Thr His Trp Phe  Val Thr Gln 
    1070                 1075                 1080             


Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe 
    1085                 1090                 1095             


Val Ser  Gly Asn Cys Asp Val  Val Ile Gly Ile Val  Asn Asn Thr 
    1100                 1105                 1110             


Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu 
    1115                 1120                 1125             


Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser Pro Asp  Val Asp Leu 
    1130                 1135                 1140             


Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys 
    1145                 1150                 1155             


Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser 
    1160                 1165                 1170             


Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys 
    1175                 1180                 1185             


Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala 
    1190                 1195                 1200             


Ile Val  Met Val Thr Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys 
    1205                 1210                 1215             


Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe 
    1220                 1225                 1230             


Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys Gly Val  Lys Leu His 
    1235                 1240                 1245             


Tyr Thr  
    1250 


<210>  64
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  64

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Glu Ile Leu Pro Val Ser 
705                 710                 715                 720 


Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser 
                725                 730                 735     


Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln 
            740                 745                 750         


Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr 
        755                 760                 765             


Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile 
    770                 775                 780                 


Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser 
785                 790                 795                 800 


Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val 
                805                 810                 815     


Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly 
            820                 825                 830         


Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu 
        835                 840                 845             


Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr 
    850                 855                 860                 


Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala 
865                 870                 875                 880 


Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe 
                885                 890                 895     


Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu 
            900                 905                 910         


Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu 
        915                 920                 925             


Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln 
    930                 935                 940                 


Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe 
945                 950                 955                 960 


Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys 
                965                 970                 975     


Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln 
            980                 985                 990         


Ser Leu Gln Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
        995                 1000                 1005             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1010                 1015                 1020             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1025                 1030                 1035             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1040                 1045                 1050             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1055                 1060                 1065             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1070                 1075                 1080             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1085                 1090                 1095             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1100                 1105                 1110             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1115                 1120                 1125             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1130                 1135                 1140             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1145                 1150                 1155             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1160                 1165                 1170             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1175                 1180                 1185             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1190                 1195                 1200             


Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val 
    1205                 1210                 1215             


Met Val  Thr Ile Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys 
    1220                 1225                 1230             


Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu 
    1235                 1240                 1245             


Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255                 1260             


<210>  65
<211>  1250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  65

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Glu Ile Leu 
    690                 695                 700                 


Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys 
705                 710                 715                 720 


Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe 
                725                 730                 735     


Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp 
            740                 745                 750         


Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr 
        755                 760                 765             


Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro 
    770                 775                 780                 


Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe 
785                 790                 795                 800 


Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp 
                805                 810                 815     


Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
            820                 825                 830         


Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala 
        835                 840                 845             


Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr 
    850                 855                 860                 


Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
865                 870                 875                 880 


Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn 
                885                 890                 895     


Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln 
            900                 905                 910         


Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val 
        915                 920                 925             


Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser 
    930                 935                 940                 


Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg 
945                 950                 955                 960 


Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly 
                965                 970                 975     


Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala 
            980                 985                 990         


Ala Glu Ile Arg Ala Ser Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu 
        995                 1000                 1005             


Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly 
    1010                 1015                 1020             


Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala Pro His  Gly Val Val 
    1025                 1030                 1035             


Phe Leu  His Val Thr Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr 
    1040                 1045                 1050             


Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys Ala His  Phe Pro Arg 
    1055                 1060                 1065             


Glu Gly  Val Phe Val Ser Asn  Gly Thr His Trp Phe  Val Thr Gln 
    1070                 1075                 1080             


Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe 
    1085                 1090                 1095             


Val Ser  Gly Asn Cys Asp Val  Val Ile Gly Ile Val  Asn Asn Thr 
    1100                 1105                 1110             


Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu 
    1115                 1120                 1125             


Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser Pro Asp  Val Asp Leu 
    1130                 1135                 1140             


Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys 
    1145                 1150                 1155             


Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser 
    1160                 1165                 1170             


Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys 
    1175                 1180                 1185             


Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala 
    1190                 1195                 1200             


Ile Val  Met Val Thr Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys 
    1205                 1210                 1215             


Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe 
    1220                 1225                 1230             


Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys Gly Val  Lys Leu His 
    1235                 1240                 1245             


Tyr Thr  
    1250 


<210>  66
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  66

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly 
            820                 825                 830         


Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu 
        835                 840                 845             


Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr 
    850                 855                 860                 


Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala 
865                 870                 875                 880 


Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe 
                885                 890                 895     


Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu 
            900                 905                 910         


Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu 
        915                 920                 925             


Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln 
    930                 935                 940                 


Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe 
945                 950                 955                 960 


Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys 
                965                 970                 975     


Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln 
            980                 985                 990         


Ser Leu Gln Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
        995                 1000                 1005             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1010                 1015                 1020             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1025                 1030                 1035             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1040                 1045                 1050             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1055                 1060                 1065             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1070                 1075                 1080             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1085                 1090                 1095             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1100                 1105                 1110             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1115                 1120                 1125             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1130                 1135                 1140             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1145                 1150                 1155             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1160                 1165                 1170             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1175                 1180                 1185             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1190                 1195                 1200             


Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val 
    1205                 1210                 1215             


Met Val  Thr Ile Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys 
    1220                 1225                 1230             


Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu 
    1235                 1240                 1245             


Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255                 1260             


<210>  67
<211>  1250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  67

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp 
                805                 810                 815     


Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
            820                 825                 830         


Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala 
        835                 840                 845             


Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr 
    850                 855                 860                 


Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
865                 870                 875                 880 


Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn 
                885                 890                 895     


Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln 
            900                 905                 910         


Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val 
        915                 920                 925             


Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser 
    930                 935                 940                 


Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg 
945                 950                 955                 960 


Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly 
                965                 970                 975     


Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala 
            980                 985                 990         


Ala Glu Ile Arg Ala Ser Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu 
        995                 1000                 1005             


Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly 
    1010                 1015                 1020             


Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala Pro His  Gly Val Val 
    1025                 1030                 1035             


Phe Leu  His Val Thr Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr 
    1040                 1045                 1050             


Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys Ala His  Phe Pro Arg 
    1055                 1060                 1065             


Glu Gly  Val Phe Val Ser Asn  Gly Thr His Trp Phe  Val Thr Gln 
    1070                 1075                 1080             


Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe 
    1085                 1090                 1095             


Val Ser  Gly Asn Cys Asp Val  Val Ile Gly Ile Val  Asn Asn Thr 
    1100                 1105                 1110             


Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu 
    1115                 1120                 1125             


Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser Pro Asp  Val Asp Leu 
    1130                 1135                 1140             


Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys 
    1145                 1150                 1155             


Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser 
    1160                 1165                 1170             


Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys 
    1175                 1180                 1185             


Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala 
    1190                 1195                 1200             


Ile Val  Met Val Thr Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys 
    1205                 1210                 1215             


Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe 
    1220                 1225                 1230             


Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys Gly Val  Lys Leu His 
    1235                 1240                 1245             


Tyr Thr  
    1250 


<210>  68
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Foldon

<400>  68

Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala 
1               5                   10                  15      


Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
            20                  25                  30      


<210>  69
<211>  202
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  69

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys 
        195                 200         


<210>  70
<211>  201
<212>  PRT
<213>  Betacoronavirus Severe acute respiratory syndrome-related coronavirus

<400>  70

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Lys Phe Pro Ser Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Ser Asn Val Tyr Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Met Gly Cys Val Leu Ala Trp Asn Thr Arg 
            100                 105                 110         


Asn Ile Asp Ala Thr Ser Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr 
        115                 120                 125             


Leu Arg His Gly Lys Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val 
    130                 135                 140                 


Pro Phe Ser Pro Asp Gly Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys 
145                 150                 155                 160 


Tyr Trp Pro Leu Asn Asp Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly 
                165                 170                 175     


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala 
            180                 185                 190         


Pro Ala Thr Val Cys Gly Pro Lys Leu 
        195                 200     


<210>  71
<211>  239
<212>  PRT
<213>  Betacoronavirus Middle East respiratory syndrome-related coronavirus

<400>  71

Phe Glu Ala Lys Pro Ser Gly Ser Val Val Ala Glu Gly Val Glu Cys 
1               5                   10                  15      


Asp Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe 
            20                  25                  30          


Lys Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu 
        35                  40                  45              


Ser Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala 
    50                  55                  60                  


Ala Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser 
65                  70                  75                  80  


Tyr Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro 
                85                  90                  95      


Ile Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu 
            100                 105                 110         


Ile Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu 
        115                 120                 125             


Lys Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg 
    130                 135                 140                 


Thr Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val 
145                 150                 155                 160 


Ser Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys 
                165                 170                 175     


Gln Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser 
            180                 185                 190         


Thr Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val 
        195                 200                 205             


Gln Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala 
    210                 215                 220                 


Asn Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr 
225                 230                 235                 


<210>  72
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  72

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
    210                 215                 220                 


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu 
225                 230                 235                 240 


Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala 
                245                 250                 255     


Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp 
            260                 265                 270         


Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr 
        275                 280                 285             


Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr 
    290                 295                 300                 


Glu Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe 
305                 310                 315                 320 


Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile 
                325                 330                 335     


Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe 
            340                 345                 350         


Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu 
        355                 360                 365             


Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu 
    370                 375                 380                 


Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn 
385                 390                 395                 400 


Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser 
                405                 410                 415     


Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg 
            420                 425                 430         


Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr 
        435                 440                 445             


Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe 
    450                 455                 460                 


Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly 
465                 470                 475                 480 


Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu 
                485                 490                 495     


His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Ala Ile Gly Gly 
            500                 505                 510         


Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp 
        515                 520                 525             


Gly Glu Trp Val Leu Leu Ser Thr Phe Leu His His His His His His 
    530                 535                 540                 


<210>  73
<211>  531
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  73

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys 
        195                 200                 205             


Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val 
    210                 215                 220                 


Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg 
225                 230                 235                 240 


Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu 
                245                 250                 255     


Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr 
            260                 265                 270         


Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val 
        275                 280                 285             


Asn Cys Thr Glu Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    290                 295                 300                 


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
305                 310                 315                 320 


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                325                 330                 335     


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            340                 345                 350         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        355                 360                 365             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    370                 375                 380                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
385                 390                 395                 400 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                405                 410                 415     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            420                 425                 430         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        435                 440                 445             


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    450                 455                 460                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
465                 470                 475                 480 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Ala 
                485                 490                 495     


Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val 
            500                 505                 510         


Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu His His His 
        515                 520                 525             


His His His 
    530     


<210>  74
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  74

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
    210                 215                 220                 


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu 
225                 230                 235                 240 


Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala 
                245                 250                 255     


Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp 
            260                 265                 270         


Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr 
        275                 280                 285             


Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr 
    290                 295                 300                 


Glu Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe 
305                 310                 315                 320 


Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile 
                325                 330                 335     


Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe 
            340                 345                 350         


Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu 
        355                 360                 365             


Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu 
    370                 375                 380                 


Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn 
385                 390                 395                 400 


Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser 
                405                 410                 415     


Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg 
            420                 425                 430         


Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr 
        435                 440                 445             


Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe 
    450                 455                 460                 


Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly 
465                 470                 475                 480 


Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu 
                485                 490                 495     


His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Ala Ile Gly Gly 
            500                 505                 510         


Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp 
        515                 520                 525             


Gly Glu Trp Val Leu Leu Ser Thr Phe Leu His His His His His His 
    530                 535                 540                 


<210>  75
<211>  531
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  75

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys 
        195                 200                 205             


Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val 
    210                 215                 220                 


Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg 
225                 230                 235                 240 


Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu 
                245                 250                 255     


Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr 
            260                 265                 270         


Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val 
        275                 280                 285             


Asn Cys Thr Glu Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    290                 295                 300                 


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
305                 310                 315                 320 


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                325                 330                 335     


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            340                 345                 350         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        355                 360                 365             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    370                 375                 380                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
385                 390                 395                 400 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                405                 410                 415     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            420                 425                 430         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        435                 440                 445             


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    450                 455                 460                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
465                 470                 475                 480 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Ala 
                485                 490                 495     


Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val 
            500                 505                 510         


Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu His His His 
        515                 520                 525             


His His His 
    530     


<210>  76
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  76

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile Thr Asn Leu 
225                 230                 235                 240 


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser Val Tyr 
                245                 250                 255     


Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
            260                 265                 270         


Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
        275                 280                 285             


Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala Asp Ser 
    290                 295                 300                 


Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly Gln Thr 
305                 310                 315                 320 


Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Met Gly 
                325                 330                 335     


Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser Thr Gly 
            340                 345                 350         


Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu Arg Pro 
        355                 360                 365             


Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly Lys Pro 
    370                 375                 380                 


Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp Tyr Gly 
385                 390                 395                 400 


Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val Val Val 
                405                 410                 415     


Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly Pro Lys 
            420                 425                 430         


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
        435                 440                 445             


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
    450                 455                 460                 


His His His His His His 
465                 470 


<210>  77
<211>  464
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  77

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile Thr Asn Leu 
225                 230                 235                 240 


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser Val Tyr 
                245                 250                 255     


Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
            260                 265                 270         


Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
        275                 280                 285             


Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala Asp Ser 
    290                 295                 300                 


Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly Gln Thr 
305                 310                 315                 320 


Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Met Gly 
                325                 330                 335     


Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser Thr Gly 
            340                 345                 350         


Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu Arg Pro 
        355                 360                 365             


Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly Lys Pro 
    370                 375                 380                 


Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp Tyr Gly 
385                 390                 395                 400 


Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val Val Val 
                405                 410                 415     


Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly Pro Lys 
            420                 425                 430         


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
        435                 440                 445             


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
    450                 455                 460                 


<210>  78
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  78

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly 
        195                 200                 205             


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile 
    210                 215                 220                 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro 
225                 230                 235                 240 


Ser Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp 
                245                 250                 255     


Tyr Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr 
            260                 265                 270         


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
        275                 280                 285             


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
    290                 295                 300                 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
305                 310                 315                 320 


Phe Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
                325                 330                 335     


Ser Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys 
            340                 345                 350         


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
        355                 360                 365             


Gly Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn 
    370                 375                 380                 


Asp Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg 
385                 390                 395                 400 


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
                405                 410                 415     


Gly Pro Lys Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg 
            420                 425                 430         


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
        435                 440                 445             


Thr Phe Leu His His His His His His 
    450                 455         


<210>  79
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  79

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly 
        195                 200                 205             


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile 
    210                 215                 220                 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro 
225                 230                 235                 240 


Ser Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp 
                245                 250                 255     


Tyr Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr 
            260                 265                 270         


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
        275                 280                 285             


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
    290                 295                 300                 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
305                 310                 315                 320 


Phe Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
                325                 330                 335     


Ser Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys 
            340                 345                 350         


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
        355                 360                 365             


Gly Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn 
    370                 375                 380                 


Asp Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg 
385                 390                 395                 400 


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
                405                 410                 415     


Gly Pro Lys Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg 
            420                 425                 430         


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
        435                 440                 445             


Thr Phe Leu 
    450     


<210>  80
<211>  726
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  80

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Ser Gly Gly Gly Ser Phe Glu Ala Lys Pro Ser Gly Ser 
225                 230                 235                 240 


Val Val Ala Glu Gly Val Glu Cys Asp Phe Ser Pro Leu Leu Ser Gly 
                245                 250                 255     


Thr Pro Pro Gln Val Tyr Asn Phe Lys Arg Leu Val Phe Thr Asn Cys 
            260                 265                 270         


Asn Tyr Asn Leu Thr Lys Leu Leu Ser Leu Phe Ser Val Asn Asp Phe 
        275                 280                 285             


Thr Cys Ser Gln Ile Ser Pro Ala Ala Ile Ala Ser Asn Cys Tyr Ser 
    290                 295                 300                 


Ser Leu Ile Leu Asp Tyr Phe Ser Tyr Pro Leu Ser Met Lys Ser Asp 
305                 310                 315                 320 


Leu Ser Val Ser Ser Ala Gly Pro Ile Ser Gln Phe Asn Tyr Lys Gln 
                325                 330                 335     


Ser Phe Ser Asn Pro Thr Cys Leu Ile Leu Ala Thr Val Pro His Asn 
            340                 345                 350         


Leu Thr Thr Ile Thr Lys Pro Leu Lys Tyr Ser Tyr Ile Asn Lys Cys 
        355                 360                 365             


Ser Arg Leu Leu Ser Asp Asp Arg Thr Glu Val Pro Gln Leu Val Asn 
    370                 375                 380                 


Ala Asn Gln Tyr Ser Pro Cys Val Ser Ile Val Pro Ser Thr Val Trp 
385                 390                 395                 400 


Glu Asp Gly Asp Tyr Tyr Arg Lys Gln Leu Ser Pro Leu Glu Gly Gly 
                405                 410                 415     


Gly Trp Leu Val Ala Ser Gly Ser Thr Val Ala Met Thr Glu Gln Leu 
            420                 425                 430         


Gln Met Gly Phe Gly Ile Thr Val Gln Tyr Gly Thr Asp Thr Asn Ser 
        435                 440                 445             


Val Cys Pro Lys Leu Glu Phe Ala Asn Asp Thr Lys Ile Ala Ser Gln 
    450                 455                 460                 


Leu Gly Asn Cys Val Glu Tyr Ser Gly Gly Gly Ser Gly Gly Gly Ser 
465                 470                 475                 480 


Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile Thr Asn Leu 
                485                 490                 495     


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser Val Tyr 
            500                 505                 510         


Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
        515                 520                 525             


Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
    530                 535                 540                 


Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala Asp Ser 
545                 550                 555                 560 


Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly Gln Thr 
                565                 570                 575     


Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Met Gly 
            580                 585                 590         


Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser Thr Gly 
        595                 600                 605             


Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu Arg Pro 
    610                 615                 620                 


Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly Lys Pro 
625                 630                 635                 640 


Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp Tyr Gly 
                645                 650                 655     


Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val Val Val 
            660                 665                 670         


Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly Pro Lys 
        675                 680                 685             


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
    690                 695                 700                 


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
705                 710                 715                 720 


His His His His His His 
                725     


<210>  81
<211>  720
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  81

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Arg Phe Pro 
1               5                   10                  15      


Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
            20                  25                  30          


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
        35                  40                  45              


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
    50                  55                  60                  


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
65                  70                  75                  80  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
                85                  90                  95      


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
            100                 105                 110         


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
        115                 120                 125             


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
    130                 135                 140                 


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
145                 150                 155                 160 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
                165                 170                 175     


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
            180                 185                 190         


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
        195                 200                 205             


Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Ser Gly Gly Gly Ser Phe Glu Ala Lys Pro Ser Gly Ser 
225                 230                 235                 240 


Val Val Ala Glu Gly Val Glu Cys Asp Phe Ser Pro Leu Leu Ser Gly 
                245                 250                 255     


Thr Pro Pro Gln Val Tyr Asn Phe Lys Arg Leu Val Phe Thr Asn Cys 
            260                 265                 270         


Asn Tyr Asn Leu Thr Lys Leu Leu Ser Leu Phe Ser Val Asn Asp Phe 
        275                 280                 285             


Thr Cys Ser Gln Ile Ser Pro Ala Ala Ile Ala Ser Asn Cys Tyr Ser 
    290                 295                 300                 


Ser Leu Ile Leu Asp Tyr Phe Ser Tyr Pro Leu Ser Met Lys Ser Asp 
305                 310                 315                 320 


Leu Ser Val Ser Ser Ala Gly Pro Ile Ser Gln Phe Asn Tyr Lys Gln 
                325                 330                 335     


Ser Phe Ser Asn Pro Thr Cys Leu Ile Leu Ala Thr Val Pro His Asn 
            340                 345                 350         


Leu Thr Thr Ile Thr Lys Pro Leu Lys Tyr Ser Tyr Ile Asn Lys Cys 
        355                 360                 365             


Ser Arg Leu Leu Ser Asp Asp Arg Thr Glu Val Pro Gln Leu Val Asn 
    370                 375                 380                 


Ala Asn Gln Tyr Ser Pro Cys Val Ser Ile Val Pro Ser Thr Val Trp 
385                 390                 395                 400 


Glu Asp Gly Asp Tyr Tyr Arg Lys Gln Leu Ser Pro Leu Glu Gly Gly 
                405                 410                 415     


Gly Trp Leu Val Ala Ser Gly Ser Thr Val Ala Met Thr Glu Gln Leu 
            420                 425                 430         


Gln Met Gly Phe Gly Ile Thr Val Gln Tyr Gly Thr Asp Thr Asn Ser 
        435                 440                 445             


Val Cys Pro Lys Leu Glu Phe Ala Asn Asp Thr Lys Ile Ala Ser Gln 
    450                 455                 460                 


Leu Gly Asn Cys Val Glu Tyr Ser Gly Gly Gly Ser Gly Gly Gly Ser 
465                 470                 475                 480 


Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile Thr Asn Leu 
                485                 490                 495     


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser Val Tyr 
            500                 505                 510         


Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
        515                 520                 525             


Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
    530                 535                 540                 


Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala Asp Ser 
545                 550                 555                 560 


Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly Gln Thr 
                565                 570                 575     


Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Met Gly 
            580                 585                 590         


Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser Thr Gly 
        595                 600                 605             


Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu Arg Pro 
    610                 615                 620                 


Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly Lys Pro 
625                 630                 635                 640 


Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp Tyr Gly 
                645                 650                 655     


Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val Val Val 
            660                 665                 670         


Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly Pro Lys 
        675                 680                 685             


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
    690                 695                 700                 


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
705                 710                 715                 720 


<210>  82
<211>  713
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  82

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly 
        195                 200                 205             


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Phe Glu Ala Lys Pro 
    210                 215                 220                 


Ser Gly Ser Val Val Ala Glu Gly Val Glu Cys Asp Phe Ser Pro Leu 
225                 230                 235                 240 


Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys Arg Leu Val Phe 
                245                 250                 255     


Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser Leu Phe Ser Val 
            260                 265                 270         


Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala Ile Ala Ser Asn 
        275                 280                 285             


Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr Pro Leu Ser Met 
    290                 295                 300                 


Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile Ser Gln Phe Asn 
305                 310                 315                 320 


Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile Leu Ala Thr Val 
                325                 330                 335     


Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys Tyr Ser Tyr Ile 
            340                 345                 350         


Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg Thr Glu Val Pro Gln 
        355                 360                 365             


Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser Ile Val Pro Ser 
    370                 375                 380                 


Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln Leu Ser Pro Leu 
385                 390                 395                 400 


Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr Val Ala Met Thr 
                405                 410                 415     


Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln Tyr Gly Thr Asp 
            420                 425                 430         


Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn Asp Thr Lys Ile 
        435                 440                 445             


Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Gly Gly Gly Ser Gly 
    450                 455                 460                 


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile 
465                 470                 475                 480 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro 
                485                 490                 495     


Ser Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp 
            500                 505                 510         


Tyr Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr 
        515                 520                 525             


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
    530                 535                 540                 


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
545                 550                 555                 560 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                565                 570                 575     


Phe Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
            580                 585                 590         


Ser Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys 
        595                 600                 605             


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
    610                 615                 620                 


Gly Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn 
625                 630                 635                 640 


Asp Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg 
                645                 650                 655     


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
            660                 665                 670         


Gly Pro Lys Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg 
        675                 680                 685             


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
    690                 695                 700                 


Thr Phe Leu His His His His His His 
705                 710             


<210>  83
<211>  707
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  83

Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
1               5                   10                  15      


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
            20                  25                  30          


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
        35                  40                  45              


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
    50                  55                  60                  


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
65                  70                  75                  80  


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
                85                  90                  95      


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
            100                 105                 110         


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
        115                 120                 125             


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
    130                 135                 140                 


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
145                 150                 155                 160 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
                165                 170                 175     


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
            180                 185                 190         


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Gly Gly Gly Ser Gly 
        195                 200                 205             


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Phe Glu Ala Lys Pro 
    210                 215                 220                 


Ser Gly Ser Val Val Ala Glu Gly Val Glu Cys Asp Phe Ser Pro Leu 
225                 230                 235                 240 


Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys Arg Leu Val Phe 
                245                 250                 255     


Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser Leu Phe Ser Val 
            260                 265                 270         


Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala Ile Ala Ser Asn 
        275                 280                 285             


Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr Pro Leu Ser Met 
    290                 295                 300                 


Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile Ser Gln Phe Asn 
305                 310                 315                 320 


Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile Leu Ala Thr Val 
                325                 330                 335     


Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys Tyr Ser Tyr Ile 
            340                 345                 350         


Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg Thr Glu Val Pro Gln 
        355                 360                 365             


Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser Ile Val Pro Ser 
    370                 375                 380                 


Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln Leu Ser Pro Leu 
385                 390                 395                 400 


Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr Val Ala Met Thr 
                405                 410                 415     


Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln Tyr Gly Thr Asp 
            420                 425                 430         


Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn Asp Thr Lys Ile 
        435                 440                 445             


Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Gly Gly Gly Ser Gly 
    450                 455                 460                 


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Arg Phe Pro Asn Ile 
465                 470                 475                 480 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro 
                485                 490                 495     


Ser Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp 
            500                 505                 510         


Tyr Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr 
        515                 520                 525             


Gly Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr 
    530                 535                 540                 


Ala Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro 
545                 550                 555                 560 


Gly Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                565                 570                 575     


Phe Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr 
            580                 585                 590         


Ser Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys 
        595                 600                 605             


Leu Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp 
    610                 615                 620                 


Gly Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn 
625                 630                 635                 640 


Asp Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg 
                645                 650                 655     


Val Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys 
            660                 665                 670         


Gly Pro Lys Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg 
        675                 680                 685             


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
    690                 695                 700                 


Thr Phe Leu 
705         


<210>  84
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  84

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  85
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  85

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  86
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  86

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  87
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  87

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  88
<211>  1219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  88

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro His His  His His His 
    1205                 1210                 1215             


His 
    


<210>  89
<211>  1206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  89

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  His His His 
    1190                 1195                 1200             


His His  His 
    1205     


<210>  90
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  90

Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  91
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  91

Ser Gly Gly Gly 
1               


<210>  92
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  92

Gly Gly Gly Ser 
1               


<210>  93
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  93

Gly Gly Gly Gly Ser 
1               5   


<210>  94
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  94

Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly 
1               5                   10                  15      


Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro 
            20                  25                  30          


Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg 
        35                  40                  45              


Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly 
    50                  55                  60                  


Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala 
65                  70                  75                  80  


Val Leu Tyr Gln Asp Val Asn Cys Thr Glu 
                85                  90  


<210>  95
<211>  3639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  95
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacgcaggg ctcgctctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcct                            3639


<210>  96
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  96
atgttcgtct tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcactc gcggtgtgta ctaccctgac      120

aaggtcttcc gttcttcagt gctgcactca actcaggacc tgttcctgcc cttcttctcc      180

aacgtcacct ggttccacgc catccacgtg tccggcacca acggcactaa gcgcttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atccgtggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtc tgcgaattcc agttctgcaa cgacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt acagcaagca caccccaatc aacctggtgc gcgacctgcc tcagggtttc      660

tctgctctgg agccactggt ggacctgcct atcggcatca acatcacccg cttccagact      720

ctgctggctc tgcaccgttc ctacctgact ccaggcgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggctacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggaa ccatcactga cgccgtcgac tgcgctctgg accctctgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggcatctacc agacttcaaa cttcagggtg      960

cagcccaccg aatccatcgt cagattccct aacatcacta acctgtgccc cttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga acaggaagag aatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggcgtgtcac caactaagct gaacgacctg tgcttcacca acgtctacgc cgactccttc     1200

gtgatcaggg gagacgaggt cagacagatc gctcctggcc agactggaaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggttgcgtca tcgcttggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtacc gcctgttccg taagtcaaac     1380

ctgaagccat tcgagaggga catcagcact gaaatctacc aggccggatc taccccttgc     1440

aacggtgtcg agggcttcaa ctgctacttc cccctgcagt cctacggttt ccagccaact     1500

aacggtgtgg gctaccagcc ttacagagtg gtcgtgctga gcttcgaact gctccacgct     1560

cctgctactg tgtgcggtcc aaagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccactg acgctgtgcg cgaccctcag     1740

accctggaaa tcctggacat cactccatgc tcattcggag gtgtctccgt gatcacccct     1800

ggcaccaaca cttctaacca ggtcgctgtg ctgtaccagg acgtcaactg caccgaggtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttggc gcgtgtactc caccggcagc     1920

aacgtgttcc agactcgtgc tggttgcctg atcggcgccg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccacagcagg ctcagtctgt ggcctctcag tcaatcatcg cttacaccat gtcactgggc     2100

gctgaaaact ccgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga accgtgctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagccaagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggattca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg tctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gcccagtaca ctagcgccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggcgccgctc tgcagatccc tttcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggaaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggcaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggtgctatca gctctgtgct gaacgacatc     2940

ctgtcccgcc tggaccctcc cgaggccgaa gtgcagatcg accgcctgat cactggccgt     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatca gggccgctga aatcagagcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggtcaatc taagcgtgtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtctgctcc ccacggtgtg     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc agggaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagag aaacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc attcaaggag gaactggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg cgacatctca ggaatcaacg cttccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggcc aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggcctt ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tctgaacccg tcctgaaggg cgtgaagctg cactacacct aa                        3822


<210>  97
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Furin Cleavage Site

<400>  97

Gly Ser Ala Ser 
1               


<210>  98
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRV3C Cleavage Site

<400>  98

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  99
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep-tag

<400>  99

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  100
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6X-His Tag

<400>  100

His His His His His His 
1               5       


<210>  101
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Tag


<220>
<221>  SITE
<222>  (6)..(6)
<223>  May be present or absent

<220>
<221>  SITE
<222>  (7)..(7)
<223>  May be present or absent

<220>
<221>  SITE
<222>  (8)..(8)
<223>  May be present or absent

<220>
<221>  SITE
<222>  (9)..(9)
<223>  May be present or absent

<220>
<221>  SITE
<222>  (10)..(10)
<223>  May be present or absent

<400>  101

His His His His His His His His His His 
1               5                   10  


<210>  102
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8X-His Tag

<400>  102

His His His His His His His His 
1               5               


<210>  103
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOLDON

<400>  103

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Ser Pro Ala 
            20                  25                  30  


<210>  104
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  104

Glu Asp Leu Leu Phe Asn Lys Val Thr Leu 
1               5                   10  


<210>  105
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  105

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly 
            820                 825                 830         


Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu 
        835                 840                 845             


Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr 
    850                 855                 860                 


Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala 
865                 870                 875                 880 


Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe 
                885                 890                 895     


Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu 
            900                 905                 910         


Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu 
        915                 920                 925             


Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln 
    930                 935                 940                 


Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe 
945                 950                 955                 960 


Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro 
                965                 970                 975     


Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln 
            980                 985                 990         


Ser Leu Gln Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
        995                 1000                 1005             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1010                 1015                 1020             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1025                 1030                 1035             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1040                 1045                 1050             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1055                 1060                 1065             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1070                 1075                 1080             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1085                 1090                 1095             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1100                 1105                 1110             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1115                 1120                 1125             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1130                 1135                 1140             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1145                 1150                 1155             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1160                 1165                 1170             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1175                 1180                 1185             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1190                 1195                 1200             


Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val 
    1205                 1210                 1215             


Met Val  Thr Ile Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys 
    1220                 1225                 1230             


Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu 
    1235                 1240                 1245             


Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255                 1260             


<210>  106
<211>  1250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  106

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp 
                805                 810                 815     


Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
            820                 825                 830         


Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala 
        835                 840                 845             


Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr 
    850                 855                 860                 


Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
865                 870                 875                 880 


Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn 
                885                 890                 895     


Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln 
            900                 905                 910         


Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val 
        915                 920                 925             


Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser 
    930                 935                 940                 


Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg 
945                 950                 955                 960 


Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly 
                965                 970                 975     


Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala 
            980                 985                 990         


Ala Glu Ile Arg Ala Ser Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu 
        995                 1000                 1005             


Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly 
    1010                 1015                 1020             


Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala Pro His  Gly Val Val 
    1025                 1030                 1035             


Phe Leu  His Val Thr Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr 
    1040                 1045                 1050             


Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys Ala His  Phe Pro Arg 
    1055                 1060                 1065             


Glu Gly  Val Phe Val Ser Asn  Gly Thr His Trp Phe  Val Thr Gln 
    1070                 1075                 1080             


Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe 
    1085                 1090                 1095             


Val Ser  Gly Asn Cys Asp Val  Val Ile Gly Ile Val  Asn Asn Thr 
    1100                 1105                 1110             


Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu 
    1115                 1120                 1125             


Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser Pro Asp  Val Asp Leu 
    1130                 1135                 1140             


Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys 
    1145                 1150                 1155             


Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser 
    1160                 1165                 1170             


Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys 
    1175                 1180                 1185             


Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala 
    1190                 1195                 1200             


Ile Val  Met Val Thr Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys 
    1205                 1210                 1215             


Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe 
    1220                 1225                 1230             


Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys Gly Val  Lys Leu His 
    1235                 1240                 1245             


Tyr Thr  
    1250 


<210>  107
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  107

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly 
            820                 825                 830         


Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu 
        835                 840                 845             


Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr 
    850                 855                 860                 


Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala 
865                 870                 875                 880 


Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe 
                885                 890                 895     


Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu 
            900                 905                 910         


Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu 
        915                 920                 925             


Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln 
    930                 935                 940                 


Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe 
945                 950                 955                 960 


Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys 
                965                 970                 975     


Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln 
            980                 985                 990         


Ser Leu Gln Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
        995                 1000                 1005             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1010                 1015                 1020             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1025                 1030                 1035             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1040                 1045                 1050             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1055                 1060                 1065             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1070                 1075                 1080             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1085                 1090                 1095             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1100                 1105                 1110             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1115                 1120                 1125             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1130                 1135                 1140             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1145                 1150                 1155             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1160                 1165                 1170             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1175                 1180                 1185             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro 
    1190                 1195                 1200             


Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val 
    1205                 1210                 1215             


Met Val  Thr Ile Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys 
    1220                 1225                 1230             


Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu 
    1235                 1240                 1245             


Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255                 1260             


<210>  108
<211>  1250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RECOMBINANT SPIKE PROTEIN

<400>  108

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp 
                805                 810                 815     


Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
            820                 825                 830         


Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala 
        835                 840                 845             


Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr 
    850                 855                 860                 


Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala 
865                 870                 875                 880 


Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn 
                885                 890                 895     


Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln 
            900                 905                 910         


Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val 
        915                 920                 925             


Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser 
    930                 935                 940                 


Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg 
945                 950                 955                 960 


Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly 
                965                 970                 975     


Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala 
            980                 985                 990         


Ala Glu Ile Arg Ala Ser Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu 
        995                 1000                 1005             


Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly 
    1010                 1015                 1020             


Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala Pro His  Gly Val Val 
    1025                 1030                 1035             


Phe Leu  His Val Thr Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr 
    1040                 1045                 1050             


Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys Ala His  Phe Pro Arg 
    1055                 1060                 1065             


Glu Gly  Val Phe Val Ser Asn  Gly Thr His Trp Phe  Val Thr Gln 
    1070                 1075                 1080             


Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe 
    1085                 1090                 1095             


Val Ser  Gly Asn Cys Asp Val  Val Ile Gly Ile Val  Asn Asn Thr 
    1100                 1105                 1110             


Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu 
    1115                 1120                 1125             


Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser Pro Asp  Val Asp Leu 
    1130                 1135                 1140             


Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys 
    1145                 1150                 1155             


Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser 
    1160                 1165                 1170             


Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys 
    1175                 1180                 1185             


Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala 
    1190                 1195                 1200             


Ile Val  Met Val Thr Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys 
    1205                 1210                 1215             


Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe 
    1220                 1225                 1230             


Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys Gly Val  Lys Leu His 
    1235                 1240                 1245             


Tyr Thr  
    1250 


<210>  109
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant CoV S polypeptide

<400>  109

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  110
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant CoV S polypeptide

<400>  110

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  111
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Furin Cleavage Site

<400>  111

Gly Ser Gly Ala 
1               


<210>  112
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 spike protein

<400>  112

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  113
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 spike protein

<400>  113

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  114
<211>  1257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 spike protein

<400>  114

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe 
    50                  55                  60                  


Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr 
65                  70                  75                  80  


Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp 
                85                  90                  95      


Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val 
            100                 105                 110         


Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val 
        115                 120                 125             


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
    130                 135                 140                 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
145                 150                 155                 160 


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
                165                 170                 175     


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
            180                 185                 190         


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
        195                 200                 205             


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
    210                 215                 220                 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
225                 230                 235                 240 


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
                245                 250                 255     


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
            260                 265                 270         


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
        275                 280                 285             


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
    290                 295                 300                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
305                 310                 315                 320 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                325                 330                 335     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            340                 345                 350         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        355                 360                 365             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    370                 375                 380                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
385                 390                 395                 400 


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                405                 410                 415     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            420                 425                 430         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        435                 440                 445             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    450                 455                 460                 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
465                 470                 475                 480 


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                485                 490                 495     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            500                 505                 510         


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
        515                 520                 525             


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
    530                 535                 540                 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val 
545                 550                 555                 560 


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
                565                 570                 575     


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
            580                 585                 590         


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
        595                 600                 605             


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
    610                 615                 620                 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
625                 630                 635                 640 


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
                645                 650                 655     


Ser Tyr Gln Thr Gln Thr Asn Ser His Gln Gln Ala Gln Ser Val Ala 
            660                 665                 670         


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
        675                 680                 685             


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile 
    690                 695                 700                 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
705                 710                 715                 720 


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
                725                 730                 735     


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
            740                 745                 750         


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
        755                 760                 765             


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
    770                 775                 780                 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
785                 790                 795                 800 


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
                805                 810                 815     


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
            820                 825                 830         


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
        835                 840                 845             


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
    850                 855                 860                 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
865                 870                 875                 880 


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
                885                 890                 895     


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
            900                 905                 910         


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
        915                 920                 925             


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
    930                 935                 940                 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
945                 950                 955                 960 


Leu Asn Asp Ile Leu Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
                965                 970                 975     


Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
            980                 985                 990         


Thr Gln Gln Leu Ile Arg Ala Ala  Glu Ile Arg Ala Ser  Ala Asn Leu 
        995                 1000                 1005             


Ala Ala  Thr Lys Met Ser Glu  Cys Val Leu Gly Gln  Ser Lys Arg 
    1010                 1015                 1020             


Val Asp  Phe Cys Gly Lys Gly  Tyr His Leu Met Ser  Phe Pro Gln 
    1025                 1030                 1035             


Ser Ala  Pro His Gly Val Val  Phe Leu His Val Thr  Tyr Val Pro 
    1040                 1045                 1050             


Ala Gln  Glu Lys Asn Phe Thr  Thr Ala Pro Ala Ile  Cys His Asp 
    1055                 1060                 1065             


Gly Lys  Ala His Phe Pro Arg  Glu Gly Val Phe Val  Ser Asn Gly 
    1070                 1075                 1080             


Thr His  Trp Phe Val Thr Gln  Arg Asn Phe Tyr Glu  Pro Gln Ile 
    1085                 1090                 1095             


Ile Thr  Thr His Asn Thr Phe  Val Ser Gly Asn Cys  Asp Val Val 
    1100                 1105                 1110             


Ile Gly  Ile Val Asn Asn Thr  Val Tyr Asp Pro Leu  Gln Pro Glu 
    1115                 1120                 1125             


Leu Asp  Ser Phe Lys Glu Glu  Leu Asp Lys Tyr Phe  Lys Asn His 
    1130                 1135                 1140             


Thr Ser  Pro Asp Val Asp Leu  Gly Asp Ile Ser Gly  Ile Asn Ala 
    1145                 1150                 1155             


Ser Val  Val Asn Ile Gln Lys  Glu Ile Asp Arg Leu  Asn Glu Val 
    1160                 1165                 1170             


Ala Lys  Asn Leu Asn Glu Ser  Leu Ile Asp Leu Gln  Glu Leu Gly 
    1175                 1180                 1185             


Lys Tyr  Glu Gln Tyr Ile Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly 
    1190                 1195                 1200             


Phe Ile  Ala Gly Leu Ile Ala  Ile Val Met Val Thr  Ile Met Leu 
    1205                 1210                 1215             


Cys Cys  Met Thr Ser Cys Cys  Ser Cys Leu Lys Gly  Cys Cys Ser 
    1220                 1225                 1230             


Cys Gly  Ser Cys Cys Lys Phe  Asp Glu Asp Asp Ser  Glu Pro Val 
    1235                 1240                 1245             


Leu Lys  Gly Val Lys Leu His  Tyr Thr 
    1250                 1255         


<210>  115
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 spike protein

<400>  115

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Ala Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu His Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  116
<211>  196
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  116

Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
1               5                   10                  15      


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
            20                  25                  30          


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
        35                  40                  45              


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
    50                  55                  60                  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
65                  70                  75                  80  


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
                85                  90                  95      


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
            100                 105                 110         


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        115                 120                 125             


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    130                 135                 140                 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
145                 150                 155                 160 


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                165                 170                 175     


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
            180                 185                 190         


Pro Lys Lys Ser 
        195     


<210>  117
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  117

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ile 
1               5                   10              


<210>  118
<211>  292
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  118

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser 
    290         


<210>  119
<211>  83
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  119

Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr 
1               5                   10                  15      


Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys 
            20                  25                  30          


Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln 
        35                  40                  45              


Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln 
    50                  55                  60                  


Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu 
65                  70                  75                  80  


Ser Arg Leu 
            


<210>  120
<211>  588
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  120

Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
1               5                   10                  15      


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
            20                  25                  30          


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
        35                  40                  45              


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
    50                  55                  60                  


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
65                  70                  75                  80  


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
                85                  90                  95      


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
            100                 105                 110         


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
        115                 120                 125             


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
    130                 135                 140                 


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
145                 150                 155                 160 


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
                165                 170                 175     


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
            180                 185                 190         


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
        195                 200                 205             


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
    210                 215                 220                 


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
225                 230                 235                 240 


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
                245                 250                 255     


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
            260                 265                 270         


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
        275                 280                 285             


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
    290                 295                 300                 


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
305                 310                 315                 320 


Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser 
                325                 330                 335     


Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser 
            340                 345                 350         


Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro 
        355                 360                 365             


Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro 
    370                 375                 380                 


Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly 
385                 390                 395                 400 


Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His 
                405                 410                 415     


Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr 
            420                 425                 430         


Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val 
        435                 440                 445             


Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys 
    450                 455                 460                 


Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp 
465                 470                 475                 480 


Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys 
                485                 490                 495     


Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu 
            500                 505                 510         


Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro 
        515                 520                 525             


Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met 
    530                 535                 540                 


Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys 
545                 550                 555                 560 


Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser 
                565                 570                 575     


Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr 
            580                 585             


<210>  121
<211>  672
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  121

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
            660                 665                 670         


<210>  122
<211>  140
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  122

Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
1               5                   10                  15      


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
            20                  25                  30          


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
        35                  40                  45              


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
    50                  55                  60                  


Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro 
65                  70                  75                  80  


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
                85                  90                  95      


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
            100                 105                 110         


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
        115                 120                 125             


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro 
    130                 135                 140 


<210>  123
<211>  24
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  123

Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met 
1               5                   10                  15      


Val Thr Ile Met Leu Cys Cys Met 
            20                  


<210>  124
<211>  36
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  124

Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys 
1               5                   10                  15      


Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu Lys Gly Val Lys 
            20                  25                  30          


Leu His Tyr Thr 
        35      


<210>  125
<211>  51
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  125

Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn 
1               5                   10                  15      


Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn 
            20                  25                  30          


Glu Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile 
        35                  40                  45              


Lys Trp Pro 
    50      


<210>  126
<211>  197
<212>  PRT
<213>  Betacoronavirus severe acute respiratory syndrome coronavirus 2

<400>  126

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro 
        195         


<210>  127
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  inactivated furin cleavage site

<400>  127

Gly Gly Gly 
1           


<210>  128
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  inactivated furin cleavage site

<400>  128

Gly Gly Gly Gly 
1               


<210>  129
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  inactivated furin cleavage site

<400>  129

Gly Gly Gly Gly Gly 
1               5   


<210>  130
<211>  1272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  130

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn 
785                 790                 795                 800 


Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe 
                805                 810                 815     


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
            820                 825                 830         


Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu 
        835                 840                 845             


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
    850                 855                 860                 


Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr 
865                 870                 875                 880 


Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
                885                 890                 895     


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
            900                 905                 910         


Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser 
        915                 920                 925             


Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 
    930                 935                 940                 


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
945                 950                 955                 960 


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
                965                 970                 975     


Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile 
            980                 985                 990         


Asp Arg Leu Ile Thr Gly Arg Leu  Gln Ser Leu Gln Thr  Tyr Val Thr 
        995                 1000                 1005             


Gln Gln  Leu Ile Arg Ala Ala  Glu Ile Arg Ala Ser  Ala Asn Leu 
    1010                 1015                 1020             


Ala Ala  Thr Lys Met Ser Glu  Cys Val Leu Gly Gln  Ser Lys Arg 
    1025                 1030                 1035             


Val Asp  Phe Cys Gly Lys Gly  Tyr His Leu Met Ser  Phe Pro Gln 
    1040                 1045                 1050             


Ser Ala  Pro His Gly Val Val  Phe Leu His Val Thr  Tyr Val Pro 
    1055                 1060                 1065             


Ala Gln  Glu Lys Asn Phe Thr  Thr Ala Pro Ala Ile  Cys His Asp 
    1070                 1075                 1080             


Gly Lys  Ala His Phe Pro Arg  Glu Gly Val Phe Val  Ser Asn Gly 
    1085                 1090                 1095             


Thr His  Trp Phe Val Thr Gln  Arg Asn Phe Tyr Glu  Pro Gln Ile 
    1100                 1105                 1110             


Ile Thr  Thr Asp Asn Thr Phe  Val Ser Gly Asn Cys  Asp Val Val 
    1115                 1120                 1125             


Ile Gly  Ile Val Asn Asn Thr  Val Tyr Asp Pro Leu  Gln Pro Glu 
    1130                 1135                 1140             


Leu Asp  Ser Phe Lys Glu Glu  Leu Asp Lys Tyr Phe  Lys Asn His 
    1145                 1150                 1155             


Thr Ser  Pro Asp Val Asp Leu  Gly Asp Ile Ser Gly  Ile Asn Ala 
    1160                 1165                 1170             


Ser Val  Val Asn Ile Gln Lys  Glu Ile Asp Arg Leu  Asn Glu Val 
    1175                 1180                 1185             


Ala Lys  Asn Leu Asn Glu Ser  Leu Ile Asp Leu Gln  Glu Leu Gly 
    1190                 1195                 1200             


Lys Tyr  Glu Gln Tyr Ile Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly 
    1205                 1210                 1215             


Phe Ile  Ala Gly Leu Ile Ala  Ile Val Met Val Thr  Ile Met Leu 
    1220                 1225                 1230             


Cys Cys  Met Thr Ser Cys Cys  Ser Cys Leu Lys Gly  Cys Cys Ser 
    1235                 1240                 1245             


Cys Gly  Ser Cys Cys Lys Phe  Asp Glu Asp Asp Ser  Glu Pro Val 
    1250                 1255                 1260             


Leu Lys  Gly Val Lys Leu His  Tyr Thr 
    1265                 1270         


<210>  131
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  131
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcacta ggggtgtgta ctacccagac      120

aaggtcttcc gctcctcagt gctgcactca actcaggacc tgttcctgcc tttcttctcc      180

aacgtcacct ggttccacgc tatccacgtg tccggcacca acggcactaa gaggttcgcc      240

aaccccgtgc tgccattcaa cgacggagtc tacttcgctt caaccgagaa gtccaacatc      300

atcagaggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcta ccaacgtggt catcaaggtg tgcgaattcc agttctgtaa cgaccccttc      420

ctgggtgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagattt acagcaagca cacccccatc aacctggtcc gtggcctgcc acagggattc      660

tctgctctgg agcccctggt ggacctgcca atcggcatca acatcaccag gttccagact      720

ctgcacgccc tgcacagatc ctacctgact cccggagact cttcatccgg ttggaccgct      780

ggcgctgccg cttactacgt gggttacctg cagccacgca ccttcctgct gaagtacaac      840

gaaaacggca ccatcactga cgccgtcgac tgcgctctgg acccactgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggaatctacc agacttcaaa cttccgcgtg      960

cagcctaccg aatccatcgt ccgtttccca aacatcacta acctgtgccc tttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga accgcaagcg tatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggagtgtcac ccactaagct gaacgacctg tgcttcacca acgtctacgc tgactccttc     1200

gtgatccgcg gtgacgaggt ccgtcagatc gctccaggcc agactggaaa catcgccgac     1260

tacaactaca agctgcctga cgacttcacc ggttgcgtca tcgcctggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtaca ggctgttcag aaagtcaaac     1380

ctgaagccct tcgagcgcga catcagcact gaaatctacc aggctggttc taccccatgc     1440

aacggtgtca agggcttcaa ctgctacttc cctctgcagt cctacggctt ccagcccact     1500

tacggtgtgg gctaccagcc ataccgtgtg gtcgtgctga gcttcgagct gctgcacgcc     1560

cctgctactg tgtgcggacc caagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggtctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cctttccagc agttcggtcg cgacatcgct gacaccactg acgccgtgcg tgaccctcag     1740

accctggaaa tcctggacat cactccctgc tcattcggag gtgtctccgt gatcactccc     1800

ggaaccaaca cttctaacca ggtcgctgtg ctgtaccagg gtgtgaactg caccgaagtc     1860

ccagtggcca tccacgctga ccagctgacc cctacttgga gggtgtactc caccggcagc     1920

aacgtcttcc agactagagc tggatgcctg atcggtgccg agcacgtcaa caacagctac     1980

gaatgcgaca tccctatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccccagcagg ctcagtctgt ggcctctcag tcaatcatcg cctacaccat gtcactgggt     2100

gtcgaaaact ccgtggctta ctctaacaac tcaatcgcca tccctaccaa cttcactatc     2160

agcgtgacca ctgagatcct gcccgtcagc atgaccaaga cttctgtgga ctgcactatg     2220

tacatctgcg gcgacagcac cgaatgctct aacctgctgc tgcagtacgg ttctttctgc     2280

acccagctga accgcgctct gactggcatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag accccaccta tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc agacccttcc aagcccagca agaggtcttt catcgaggac     2460

ctgctgttca acaaggtcac tctggccgac gctggtttca tcaagcagta cggcgactgc     2520

ctgggagaca tcgctgctcg cgacctgatc tgcgcccaga agttcaacgg cctgaccgtg     2580

ctgcccccac tgctgactga cgaaatgatc gctcagtaca ctagcgccct gctggctggc     2640

accatcactt caggttggac tttcggtgct ggtgctgctc tgcagatccc attcgctatg     2700

cagatggcct accgtttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactcagcc atcggcaaga tccaggacag cctgagctct     2820

actgcctctg ctctgggaaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggcgccatca gctctgtgct gaacgacatc     2940

ctgtccaggc tggaccctcc cgaggctgaa gtccagatcg acaggctgat cactggaaga     3000

ctgcagtcac tgcagaccta cgtgactcag cagctgatcc gcgccgctga aatccgtgcc     3060

tccgctaacc tggccgctac caagatgagc gagtgcgtcc tgggccagtc taagagagtg     3120

gacttctgcg gcaagggata ccacctgatg tcattcccac agtccgcccc tcacggagtc     3180

gtgttcctgc acgtcaccta cgtgcccgct caggagaaga acttcaccac tgccccagct     3240

atctgccacg acggcaaggc tcacttccct cgcgaaggag tcttcgtgag caacggcacc     3300

cactggttcg tcactcagcg taacttctac gagccccaga tcatcaccac tgacaacact     3360

ttcgtgtctg gcaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgaccct     3420

ctgcagcccg agctggactc attcaaggaa gagctggaca agtacttcaa gaaccacacc     3480

tcccctgacg tcgacctggg tgacatctca ggcatcaacg cctccgtcgt gaacatccag     3540

aaggagatcg accgcctgaa cgaagtggct aagaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggccat ggtacatctg gctgggattc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggttg ctgcagctgc ggctcttgct gcaagttcga cgaggacgac     3780

tctgaacctg tcctgaaggg agtgaagctg cactacacct aa                        3822


<210>  132
<211>  1259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  132

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Ala Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu His Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
785                 790                 795                 800 


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
                805                 810                 815     


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
            820                 825                 830         


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
        835                 840                 845             


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
    850                 855                 860                 


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
865                 870                 875                 880 


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
                885                 890                 895     


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
            900                 905                 910         


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
        915                 920                 925             


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
    930                 935                 940                 


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
945                 950                 955                 960 


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu 
                965                 970                 975     


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
            980                 985                 990         


Tyr Val Thr Gln Gln Leu Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala 
        995                 1000                 1005             


Asn Leu  Ala Ala Thr Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser 
    1010                 1015                 1020             


Lys Arg  Val Asp Phe Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe 
    1025                 1030                 1035             


Pro Gln  Ser Ala Pro His Gly  Val Val Phe Leu His  Val Thr Tyr 
    1040                 1045                 1050             


Val Pro  Ala Gln Glu Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys 
    1055                 1060                 1065             


His Asp  Gly Lys Ala His Phe  Pro Arg Glu Gly Val  Phe Val Ser 
    1070                 1075                 1080             


Asn Gly  Thr His Trp Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro 
    1085                 1090                 1095             


Gln Ile  Ile Thr Thr Asp Asn  Thr Phe Val Ser Gly  Asn Cys Asp 
    1100                 1105                 1110             


Val Val  Ile Gly Ile Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln 
    1115                 1120                 1125             


Pro Glu  Leu Asp Ser Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys 
    1130                 1135                 1140             


Asn His  Thr Ser Pro Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile 
    1145                 1150                 1155             


Asn Ala  Ser Val Val Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn 
    1160                 1165                 1170             


Glu Val  Ala Lys Asn Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu 
    1175                 1180                 1185             


Leu Gly  Lys Tyr Glu Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp 
    1190                 1195                 1200             


Leu Gly  Phe Ile Ala Gly Leu  Ile Ala Ile Val Met  Val Thr Ile 
    1205                 1210                 1215             


Met Leu  Cys Cys Met Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys 
    1220                 1225                 1230             


Cys Ser  Cys Gly Ser Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu 
    1235                 1240                 1245             


Pro Val  Leu Lys Gly Val Lys  Leu His Tyr Thr 
    1250                 1255                 


<210>  133
<211>  1258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  133

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Gly Ser Val 
            660                 665                 670         


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn 
        675                 680                 685             


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
    690                 695                 700                 


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
705                 710                 715                 720 


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
                725                 730                 735     


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
            740                 745                 750         


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
        755                 760                 765             


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
    770                 775                 780                 


Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg 
785                 790                 795                 800 


Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
                805                 810                 815     


Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg 
            820                 825                 830         


Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro 
        835                 840                 845             


Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala 
    850                 855                 860                 


Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln 
865                 870                 875                 880 


Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val 
                885                 890                 895     


Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe 
            900                 905                 910         


Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser 
        915                 920                 925             


Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu 
    930                 935                 940                 


Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
945                 950                 955                 960 


Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val 
                965                 970                 975     


Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr 
            980                 985                 990         


Val Thr Gln Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
        995                 1000                 1005             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1010                 1015                 1020             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1025                 1030                 1035             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1040                 1045                 1050             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1055                 1060                 1065             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1070                 1075                 1080             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1085                 1090                 1095             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1100                 1105                 1110             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1115                 1120                 1125             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1130                 1135                 1140             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1145                 1150                 1155             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1160                 1165                 1170             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1175                 1180                 1185             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1190                 1195                 1200             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1205                 1210                 1215             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1220                 1225                 1230             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1235                 1240                 1245             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255             


<210>  134
<211>  1271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  134

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Gly Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
785                 790                 795                 800 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
                805                 810                 815     


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
            820                 825                 830         


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
        835                 840                 845             


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
    850                 855                 860                 


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
865                 870                 875                 880 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
                885                 890                 895     


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
            900                 905                 910         


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
        915                 920                 925             


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
    930                 935                 940                 


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
945                 950                 955                 960 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
                965                 970                 975     


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
            980                 985                 990         


Arg Leu Ile Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
        995                 1000                 1005             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1010                 1015                 1020             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1025                 1030                 1035             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1040                 1045                 1050             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1055                 1060                 1065             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1070                 1075                 1080             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1085                 1090                 1095             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1100                 1105                 1110             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1115                 1120                 1125             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1130                 1135                 1140             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1145                 1150                 1155             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1160                 1165                 1170             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1175                 1180                 1185             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1190                 1195                 1200             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1205                 1210                 1215             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1220                 1225                 1230             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1235                 1240                 1245             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1250                 1255                 1260             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1265                 1270     


<210>  135
<211>  3813
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  135

Ala Thr Gly Thr Thr Cys Gly Thr Cys Thr Thr Cys Cys Thr Gly Gly 
1               5                   10                  15      


Thr Gly Cys Thr Gly Cys Thr Gly Cys Cys Thr Cys Thr Gly Gly Thr 
            20                  25                  30          


Gly Thr Cys Cys Ala Gly Cys Cys Ala Gly Thr Gly Cys Gly Thr Gly 
        35                  40                  45              


Ala Ala Cys Cys Thr Gly Ala Cys Cys Ala Cys Thr Ala Gly Gly Ala 
    50                  55                  60                  


Cys Thr Cys Ala Gly Cys Thr Gly Cys Cys Thr Cys Cys Cys Gly Cys 
65                  70                  75                  80  


Thr Thr Ala Cys Ala Cys Cys Ala Ala Cys Ala Gly Cys Thr Thr Cys 
                85                  90                  95      


Ala Cys Thr Ala Gly Gly Gly Gly Thr Gly Thr Cys Thr Ala Cys Thr 
            100                 105                 110         


Ala Cys Cys Cys Thr Gly Ala Cys Ala Ala Gly Gly Thr Cys Thr Thr 
        115                 120                 125             


Cys Cys Gly Cys Thr Cys Cys Thr Cys Ala Gly Thr Gly Cys Thr Gly 
    130                 135                 140                 


Cys Ala Cys Thr Cys Thr Ala Cys Thr Cys Ala Gly Gly Ala Cys Cys 
145                 150                 155                 160 


Thr Gly Thr Thr Cys Cys Thr Gly Cys Cys Cys Thr Thr Cys Thr Thr 
                165                 170                 175     


Cys Thr Cys Ala Ala Ala Cys Gly Thr Gly Ala Cys Cys Thr Gly Gly 
            180                 185                 190         


Thr Thr Cys Cys Ala Cys Gly Cys Cys Ala Thr Cys Thr Cys Cys Gly 
        195                 200                 205             


Gly Cys Ala Cys Cys Ala Ala Cys Gly Gly Cys Ala Cys Thr Ala Ala 
    210                 215                 220                 


Gly Ala Gly Gly Thr Thr Cys Gly Ala Cys Ala Ala Cys Cys Cys Cys 
225                 230                 235                 240 


Gly Thr Gly Cys Thr Gly Cys Cys Ala Thr Thr Cys Ala Ala Cys Gly 
                245                 250                 255     


Ala Cys Gly Gly Cys Gly Thr Cys Thr Ala Cys Thr Thr Cys Gly Cys 
            260                 265                 270         


Thr Thr Cys Ala Ala Cys Cys Gly Ala Gly Ala Ala Gly Thr Cys Cys 
        275                 280                 285             


Ala Ala Cys Ala Thr Cys Ala Thr Cys Ala Gly Ala Gly Gly Ala Thr 
    290                 295                 300                 


Gly Gly Ala Thr Cys Thr Thr Cys Gly Gly Cys Ala Cys Cys Ala Cys 
305                 310                 315                 320 


Thr Cys Thr Gly Gly Ala Cys Ala Gly Cys Ala Ala Gly Ala Cys Thr 
                325                 330                 335     


Cys Ala Gly Thr Cys Thr Cys Thr Gly Cys Thr Gly Ala Thr Cys Gly 
            340                 345                 350         


Thr Cys Ala Ala Cys Ala Ala Cys Gly Cys Cys Ala Cys Cys Ala Ala 
        355                 360                 365             


Cys Gly Thr Gly Gly Thr Cys Ala Thr Cys Ala Ala Gly Gly Thr Gly 
    370                 375                 380                 


Thr Gly Cys Gly Ala Ala Thr Thr Cys Cys Ala Gly Thr Thr Cys Thr 
385                 390                 395                 400 


Gly Cys Ala Ala Cys Gly Ala Cys Cys Cys Ala Thr Thr Cys Cys Thr 
                405                 410                 415     


Gly Gly Gly Thr Gly Thr Gly Thr Ala Cys Cys Ala Cys Ala Ala Gly 
            420                 425                 430         


Ala Ala Cys Ala Ala Cys Ala Ala Gly Thr Cys Ala Thr Gly Gly Ala 
        435                 440                 445             


Thr Gly Gly Ala Gly Thr Cys Cys Gly Ala Ala Thr Thr Cys Cys Gly 
    450                 455                 460                 


Cys Gly Thr Cys Thr Ala Cys Thr Cys Cys Ala Gly Cys Gly Cys Thr 
465                 470                 475                 480 


Ala Ala Cys Ala Ala Cys Thr Gly Cys Ala Cys Thr Thr Thr Cys Gly 
                485                 490                 495     


Ala Gly Thr Ala Cys Gly Thr Gly Ala Gly Cys Cys Ala Gly Cys Cys 
            500                 505                 510         


Thr Thr Thr Cys Cys Thr Gly Ala Thr Gly Gly Ala Cys Cys Thr Gly 
        515                 520                 525             


Gly Ala Ala Gly Gly Ala Ala Ala Gly Cys Ala Gly Gly Gly Thr Ala 
    530                 535                 540                 


Ala Cys Thr Thr Cys Ala Ala Gly Ala Ala Cys Cys Thr Gly Cys Gly 
545                 550                 555                 560 


Cys Gly Ala Gly Thr Thr Cys Gly Thr Gly Thr Thr Cys Ala Ala Gly 
                565                 570                 575     


Ala Ala Cys Ala Thr Cys Gly Ala Cys Gly Gly Ala Thr Ala Cys Thr 
            580                 585                 590         


Thr Cys Ala Ala Gly Ala Thr Thr Thr Ala Cys Ala Gly Cys Ala Ala 
        595                 600                 605             


Gly Cys Ala Cys Ala Cys Cys Cys Cys Cys Ala Thr Cys Ala Ala Cys 
    610                 615                 620                 


Cys Thr Gly Gly Thr Cys Cys Gly Thr Gly Ala Cys Cys Thr Gly Cys 
625                 630                 635                 640 


Cys Ala Cys Ala Gly Gly Gly Thr Thr Thr Cys Thr Cys Thr Gly Cys 
                645                 650                 655     


Thr Cys Thr Gly Gly Ala Gly Cys Cys Cys Cys Thr Gly Gly Thr Gly 
            660                 665                 670         


Gly Ala Cys Cys Thr Gly Cys Cys Ala Ala Thr Cys Gly Gly Cys Ala 
        675                 680                 685             


Thr Cys Ala Ala Cys Ala Thr Cys Ala Cys Cys Ala Gly Gly Thr Thr 
    690                 695                 700                 


Cys Cys Ala Gly Ala Cys Thr Cys Thr Gly Cys Thr Gly Gly Cys Thr 
705                 710                 715                 720 


Cys Thr Gly Cys Ala Cys Cys Gly Cys Thr Cys Cys Thr Ala Cys Cys 
                725                 730                 735     


Thr Gly Ala Cys Thr Cys Cys Ala Gly Gly Thr Gly Ala Cys Thr Cys 
            740                 745                 750         


Ala Thr Cys Thr Thr Cys Thr Gly Gly Ala Thr Gly Gly Ala Cys Thr 
        755                 760                 765             


Gly Cys Thr Gly Gly Ala Gly Cys Thr Gly Cys Thr Gly Cys Thr Thr 
    770                 775                 780                 


Ala Cys Thr Ala Cys Gly Thr Gly Gly Gly Thr Thr Ala Cys Cys Thr 
785                 790                 795                 800 


Gly Cys Ala Gly Cys Cys Thr Cys Gly Cys Ala Cys Cys Thr Thr Cys 
                805                 810                 815     


Cys Thr Gly Cys Thr Gly Ala Ala Gly Thr Ala Cys Ala Ala Cys Gly 
            820                 825                 830         


Ala Ala Ala Ala Cys Gly Gly Cys Ala Cys Cys Ala Thr Cys Ala Cys 
        835                 840                 845             


Thr Gly Ala Cys Gly Cys Cys Gly Thr Cys Gly Ala Cys Thr Gly Cys 
    850                 855                 860                 


Gly Cys Thr Cys Thr Gly Gly Ala Cys Cys Cys Thr Cys Thr Gly Thr 
865                 870                 875                 880 


Cys Ala Gly Ala Ala Ala Cys Cys Ala Ala Gly Thr Gly Cys Ala Cys 
                885                 890                 895     


Thr Cys Thr Gly Ala Ala Gly Thr Cys Cys Thr Thr Cys Ala Cys Cys 
            900                 905                 910         


Gly Thr Gly Gly Ala Gly Ala Ala Gly Gly Gly Thr Ala Thr Cys Thr 
        915                 920                 925             


Ala Cys Cys Ala Gly Ala Cys Thr Ala Gly Cys Ala Ala Cys Thr Thr 
    930                 935                 940                 


Cys Cys Gly Cys Gly Thr Gly Cys Ala Gly Cys Cys Cys Ala Cys Cys 
945                 950                 955                 960 


Gly Ala Ala Thr Cys Thr Ala Thr Cys Gly Thr Cys Cys Gly Thr Thr 
                965                 970                 975     


Thr Cys Cys Cys Ala Ala Ala Cys Ala Thr Cys Ala Cys Thr Ala Ala 
            980                 985                 990         


Cys Cys Thr Gly Thr Gly Cys Cys  Cys Thr Thr Thr Cys  Gly Gly Cys 
        995                 1000                 1005             


Gly Ala  Gly Gly Thr Cys Thr  Thr Cys Ala Ala Cys  Gly Cys Cys 
    1010                 1015                 1020             


Ala Cys  Cys Cys Gly Cys Thr  Thr Cys Gly Cys Thr  Thr Cys Cys 
    1025                 1030                 1035             


Gly Thr  Gly Thr Ala Cys Gly  Cys Cys Thr Gly Gly  Ala Ala Cys 
    1040                 1045                 1050             


Cys Gly  Cys Ala Ala Gly Cys  Gly Thr Ala Thr Cys  Thr Cys Ala 
    1055                 1060                 1065             


Ala Ala  Cys Thr Gly Cys Gly  Thr Cys Gly Cys Thr  Gly Ala Cys 
    1070                 1075                 1080             


Thr Ala  Cys Thr Cys Cys Gly  Thr Gly Cys Thr Gly  Thr Ala Cys 
    1085                 1090                 1095             


Ala Ala  Cys Thr Cys Ala Gly  Cys Cys Thr Cys Cys  Thr Thr Cys 
    1100                 1105                 1110             


Ala Gly  Cys Ala Cys Cys Thr  Thr Cys Ala Ala Gly  Thr Gly Cys 
    1115                 1120                 1125             


Thr Ala  Cys Gly Gly Thr Gly  Thr Gly Thr Cys Ala  Cys Cys Cys 
    1130                 1135                 1140             


Ala Cys  Thr Ala Ala Gly Cys  Thr Gly Ala Ala Cys  Gly Ala Cys 
    1145                 1150                 1155             


Cys Thr  Gly Thr Gly Cys Thr  Thr Cys Ala Cys Cys  Ala Ala Cys 
    1160                 1165                 1170             


Gly Thr  Cys Thr Ala Cys Gly  Cys Cys Gly Ala Cys  Thr Cys Cys 
    1175                 1180                 1185             


Thr Thr  Cys Gly Thr Gly Ala  Thr Cys Cys Gly Cys  Gly Gly Cys 
    1190                 1195                 1200             


Gly Ala  Cys Gly Ala Gly Gly  Thr Cys Cys Gly Thr  Cys Ala Gly 
    1205                 1210                 1215             


Ala Thr  Cys Gly Cys Thr Cys  Cys Ala Gly Gly Cys  Cys Ala Gly 
    1220                 1225                 1230             


Ala Cys  Thr Gly Gly Ala Ala  Ala Gly Ala Thr Cys  Gly Cys Cys 
    1235                 1240                 1245             


Gly Ala  Cys Thr Ala Cys Ala  Ala Cys Thr Ala Cys  Ala Ala Gly 
    1250                 1255                 1260             


Cys Thr  Gly Cys Cys Thr Gly  Ala Cys Gly Ala Cys  Thr Thr Cys 
    1265                 1270                 1275             


Ala Cys  Cys Gly Gly Cys Thr  Gly Cys Gly Thr Cys  Ala Thr Cys 
    1280                 1285                 1290             


Gly Cys  Thr Thr Gly Gly Ala  Ala Cys Ala Gly Cys  Ala Ala Cys 
    1295                 1300                 1305             


Ala Ala  Cys Cys Thr Gly Gly  Ala Cys Thr Cys Thr  Ala Ala Ala 
    1310                 1315                 1320             


Gly Thr  Gly Gly Gly Thr Gly  Gly Cys Ala Ala Cys  Thr Ala Cys 
    1325                 1330                 1335             


Ala Ala  Cys Thr Ala Cys Cys  Thr Gly Thr Ala Cys  Ala Gly Gly 
    1340                 1345                 1350             


Cys Thr  Gly Thr Thr Cys Ala  Gly Ala Ala Ala Gly  Ala Gly Cys 
    1355                 1360                 1365             


Ala Ala  Cys Cys Thr Gly Ala  Ala Gly Cys Cys Cys  Thr Thr Cys 
    1370                 1375                 1380             


Gly Ala  Gly Cys Gly Cys Gly  Ala Cys Ala Thr Cys  Ala Gly Cys 
    1385                 1390                 1395             


Ala Cys  Thr Gly Ala Ala Ala  Thr Cys Thr Ala Cys  Cys Ala Gly 
    1400                 1405                 1410             


Gly Cys  Thr Gly Gly Cys Thr  Cys Thr Ala Cys Cys  Cys Cys Ala 
    1415                 1420                 1425             


Thr Gly  Cys Ala Ala Cys Gly  Gly Thr Gly Thr Cys  Gly Ala Gly 
    1430                 1435                 1440             


Gly Gly  Cys Thr Thr Cys Ala  Ala Cys Thr Gly Cys  Thr Ala Cys 
    1445                 1450                 1455             


Thr Thr  Cys Cys Cys Thr Cys  Thr Gly Cys Ala Gly  Thr Cys Thr 
    1460                 1465                 1470             


Thr Ala  Cys Gly Gly Ala Thr  Thr Cys Cys Ala Gly  Cys Cys Cys 
    1475                 1480                 1485             


Ala Cys  Thr Thr Ala Cys Gly  Gly Thr Gly Thr Gly  Gly Gly Cys 
    1490                 1495                 1500             


Thr Ala  Cys Cys Ala Gly Cys  Cys Ala Thr Ala Cys  Cys Gly Thr 
    1505                 1510                 1515             


Gly Thr  Gly Gly Thr Cys Gly  Thr Gly Cys Thr Gly  Ala Gly Cys 
    1520                 1525                 1530             


Thr Thr  Cys Gly Ala Ala Cys  Thr Gly Cys Thr Gly  Cys Ala Cys 
    1535                 1540                 1545             


Gly Cys  Cys Cys Cys Thr Gly  Cys Thr Ala Cys Thr  Gly Thr Gly 
    1550                 1555                 1560             


Thr Gly  Cys Gly Gly Ala Cys  Cys Cys Ala Ala Gly  Ala Ala Gly 
    1565                 1570                 1575             


Thr Cys  Thr Ala Cys Cys Ala  Ala Cys Cys Thr Gly  Gly Thr Cys 
    1580                 1585                 1590             


Ala Ala  Gly Ala Ala Cys Ala  Ala Gly Thr Gly Cys  Gly Thr Gly 
    1595                 1600                 1605             


Ala Ala  Cys Thr Thr Cys Ala  Ala Cys Thr Thr Cys  Ala Ala Cys 
    1610                 1615                 1620             


Gly Gly  Thr Cys Thr Gly Ala  Cys Cys Gly Gly Ala  Ala Cys Thr 
    1625                 1630                 1635             


Gly Gly  Thr Gly Thr Cys Cys  Thr Gly Ala Cys Cys  Gly Ala Gly 
    1640                 1645                 1650             


Ala Gly  Cys Ala Ala Cys Ala  Ala Gly Ala Ala Gly  Thr Thr Cys 
    1655                 1660                 1665             


Cys Thr  Gly Cys Cys Cys Thr  Thr Cys Cys Ala Gly  Cys Ala Gly 
    1670                 1675                 1680             


Thr Thr  Cys Gly Gly Thr Cys  Gly Cys Gly Ala Cys  Ala Thr Cys 
    1685                 1690                 1695             


Gly Ala  Cys Gly Ala Cys Ala  Cys Cys Ala Cys Thr  Gly Ala Cys 
    1700                 1705                 1710             


Gly Cys  Cys Gly Thr Gly Cys  Gly Thr Gly Ala Cys  Cys Cys Thr 
    1715                 1720                 1725             


Cys Ala  Gly Ala Cys Cys Cys  Thr Gly Gly Ala Ala  Ala Thr Cys 
    1730                 1735                 1740             


Cys Thr  Gly Gly Ala Cys Ala  Thr Cys Ala Cys Thr  Cys Cys Cys 
    1745                 1750                 1755             


Thr Gly  Cys Thr Cys Ala Thr  Thr Cys Gly Gly Ala  Gly Gly Thr 
    1760                 1765                 1770             


Gly Thr  Cys Thr Cys Cys Gly  Thr Gly Ala Thr Cys  Ala Cys Thr 
    1775                 1780                 1785             


Cys Cys  Ala Gly Gly Ala Ala  Cys Cys Ala Ala Cys  Ala Cys Thr 
    1790                 1795                 1800             


Thr Cys  Thr Ala Ala Cys Cys  Ala Gly Gly Thr Cys  Gly Cys Thr 
    1805                 1810                 1815             


Gly Thr  Gly Cys Thr Gly Thr  Ala Cys Cys Ala Gly  Gly Gly Thr 
    1820                 1825                 1830             


Gly Thr  Cys Ala Ala Cys Thr  Gly Cys Ala Cys Cys  Gly Ala Gly 
    1835                 1840                 1845             


Gly Thr  Cys Cys Cys Ala Gly  Thr Gly Gly Cys Cys  Ala Thr Cys 
    1850                 1855                 1860             


Cys Ala  Cys Gly Cys Thr Gly  Ala Cys Cys Ala Gly  Cys Thr Gly 
    1865                 1870                 1875             


Ala Cys  Cys Cys Cys Thr Ala  Cys Thr Thr Gly Gly  Ala Gly Gly 
    1880                 1885                 1890             


Gly Thr  Gly Thr Ala Cys Thr  Cys Cys Ala Cys Cys  Gly Gly Cys 
    1895                 1900                 1905             


Ala Gly  Cys Ala Ala Cys Gly  Thr Gly Thr Thr Cys  Cys Ala Gly 
    1910                 1915                 1920             


Ala Cys  Thr Ala Gly Ala Gly  Cys Thr Gly Gly Thr  Thr Gly Cys 
    1925                 1930                 1935             


Cys Thr  Gly Ala Thr Cys Gly  Gly Cys Gly Cys Cys  Gly Ala Gly 
    1940                 1945                 1950             


Cys Ala  Cys Gly Thr Gly Ala  Ala Cys Ala Ala Cys  Ala Gly Cys 
    1955                 1960                 1965             


Thr Ala  Cys Gly Ala Ala Thr  Gly Cys Gly Ala Cys  Ala Thr Cys 
    1970                 1975                 1980             


Cys Cys  Ala Ala Thr Cys Gly  Gly Cys Gly Cys Thr  Gly Gly Ala 
    1985                 1990                 1995             


Ala Thr  Cys Thr Gly Cys Gly  Cys Cys Thr Cys Thr  Thr Ala Cys 
    2000                 2005                 2010             


Cys Ala  Gly Ala Cys Cys Cys  Ala Gly Ala Cys Thr  Ala Ala Cys 
    2015                 2020                 2025             


Ala Gly  Cys Cys Ala Cys Cys  Ala Gly Cys Ala Gly  Gly Cys Thr 
    2030                 2035                 2040             


Cys Ala  Gly Thr Cys Thr Gly  Thr Cys Gly Cys Cys  Thr Cys Thr 
    2045                 2050                 2055             


Cys Ala  Gly Thr Cys Ala Ala  Thr Cys Ala Thr Cys  Gly Cys Thr 
    2060                 2065                 2070             


Thr Ala  Cys Ala Cys Thr Ala  Thr Gly Thr Cys Ala  Cys Thr Gly 
    2075                 2080                 2085             


Gly Gly  Cys Gly Cys Thr Gly  Ala Ala Ala Ala Cys  Thr Cys Cys 
    2090                 2095                 2100             


Gly Thr  Gly Gly Cys Cys Thr  Ala Cys Thr Cys Thr  Ala Ala Cys 
    2105                 2110                 2115             


Ala Ala  Cys Thr Cys Ala Ala  Thr Cys Gly Cys Cys  Ala Thr Cys 
    2120                 2125                 2130             


Cys Cys  Thr Ala Thr Cys Ala  Ala Cys Thr Thr Cys  Ala Cys Cys 
    2135                 2140                 2145             


Ala Thr  Cys Thr Cys Ala Gly  Thr Gly Ala Cys Cys  Ala Cys Thr 
    2150                 2155                 2160             


Gly Ala  Gly Ala Thr Cys Cys  Thr Gly Cys Cys Cys  Gly Thr Cys 
    2165                 2170                 2175             


Ala Gly  Cys Ala Thr Gly Ala  Cys Cys Ala Ala Gly  Ala Cys Thr 
    2180                 2185                 2190             


Thr Cys  Thr Gly Thr Gly Gly  Ala Cys Thr Gly Cys  Ala Cys Thr 
    2195                 2200                 2205             


Ala Thr  Gly Thr Ala Cys Ala  Thr Cys Thr Gly Cys  Gly Gly Ala 
    2210                 2215                 2220             


Gly Ala  Cys Ala Gly Cys Ala  Cys Cys Gly Ala Ala  Thr Gly Cys 
    2225                 2230                 2235             


Thr Cys  Thr Ala Ala Cys Cys  Thr Gly Cys Thr Gly  Cys Thr Gly 
    2240                 2245                 2250             


Cys Ala  Gly Thr Ala Cys Gly  Gly Cys Thr Cys Cys  Thr Thr Cys 
    2255                 2260                 2265             


Thr Gly  Cys Ala Cys Cys Cys  Ala Gly Cys Thr Gly  Ala Ala Cys 
    2270                 2275                 2280             


Cys Gly  Cys Gly Cys Thr Cys  Thr Gly Ala Cys Thr  Gly Gly Ala 
    2285                 2290                 2295             


Ala Thr  Cys Gly Cys Cys Gly  Thr Gly Gly Ala Gly  Cys Ala Gly 
    2300                 2305                 2310             


Gly Ala  Cys Ala Ala Gly Ala  Ala Cys Ala Cys Thr  Cys Ala Gly 
    2315                 2320                 2325             


Gly Ala  Ala Gly Thr Cys Thr  Thr Cys Gly Cys Thr  Cys Ala Gly 
    2330                 2335                 2340             


Gly Thr  Gly Ala Ala Gly Cys  Ala Ala Ala Thr Cys  Thr Ala Cys 
    2345                 2350                 2355             


Ala Ala  Gly Ala Cys Cys Cys  Cys Ala Cys Cys Thr  Ala Thr Cys 
    2360                 2365                 2370             


Ala Ala  Gly Gly Ala Cys Thr  Thr Cys Gly Gly Cys  Gly Gly Ala 
    2375                 2380                 2385             


Thr Thr  Cys Ala Ala Cys Thr  Thr Cys Thr Cys Cys  Cys Ala Gly 
    2390                 2395                 2400             


Ala Thr  Cys Cys Thr Gly Cys  Cys Ala Gly Ala Cys  Cys Cys Thr 
    2405                 2410                 2415             


Thr Cys  Cys Ala Ala Gly Cys  Cys Ala Ala Gly Cys  Ala Ala Gly 
    2420                 2425                 2430             


Ala Gly  Gly Thr Cys Thr Thr  Thr Cys Ala Thr Cys  Gly Ala Gly 
    2435                 2440                 2445             


Gly Ala  Cys Cys Thr Gly Cys  Thr Gly Thr Thr Cys  Ala Ala Cys 
    2450                 2455                 2460             


Ala Ala  Gly Gly Thr Cys Ala  Cys Cys Cys Thr Gly  Gly Cys Cys 
    2465                 2470                 2475             


Gly Ala  Cys Gly Cys Thr Gly  Gly Cys Thr Thr Cys  Ala Thr Cys 
    2480                 2485                 2490             


Ala Ala  Gly Cys Ala Gly Thr  Ala Cys Gly Gly Ala  Gly Ala Cys 
    2495                 2500                 2505             


Thr Gly  Cys Cys Thr Gly Gly  Gly Thr Gly Ala Cys  Ala Thr Cys 
    2510                 2515                 2520             


Gly Cys  Cys Gly Cys Thr Cys  Gly Cys Gly Ala Cys  Cys Thr Gly 
    2525                 2530                 2535             


Ala Thr  Cys Thr Gly Cys Gly  Cys Thr Cys Ala Gly  Ala Ala Gly 
    2540                 2545                 2550             


Thr Thr  Cys Ala Ala Cys Gly  Gly Ala Cys Thr Gly  Ala Cys Cys 
    2555                 2560                 2565             


Gly Thr  Gly Cys Thr Gly Cys  Cys Cys Cys Cys Ala  Cys Thr Gly 
    2570                 2575                 2580             


Cys Thr  Gly Ala Cys Thr Gly  Ala Cys Gly Ala Ala  Ala Thr Gly 
    2585                 2590                 2595             


Ala Thr  Cys Gly Cys Cys Cys  Ala Gly Thr Ala Cys  Ala Cys Thr 
    2600                 2605                 2610             


Thr Cys  Ala Gly Cys Cys Cys  Thr Gly Cys Thr Gly  Gly Cys Thr 
    2615                 2620                 2625             


Gly Gly  Ala Ala Cys Cys Ala  Thr Cys Ala Cys Thr  Thr Cys Thr 
    2630                 2635                 2640             


Gly Gly  Thr Thr Gly Gly Ala  Cys Cys Thr Thr Cys  Gly Gly Thr 
    2645                 2650                 2655             


Gly Cys  Thr Gly Gly Thr Gly  Cys Thr Gly Cys Thr  Cys Thr Gly 
    2660                 2665                 2670             


Cys Ala  Gly Ala Thr Cys Cys  Cys Thr Thr Thr Cys  Gly Cys Thr 
    2675                 2680                 2685             


Ala Thr  Gly Cys Ala Gly Ala  Thr Gly Gly Cys Cys  Thr Ala Cys 
    2690                 2695                 2700             


Cys Gly  Thr Thr Thr Cys Ala  Ala Cys Gly Gly Ala  Ala Thr Cys 
    2705                 2710                 2715             


Gly Gly  Thr Gly Thr Cys Ala  Cys Cys Cys Ala Gly  Ala Ala Cys 
    2720                 2725                 2730             


Gly Thr  Gly Cys Thr Gly Thr  Ala Cys Gly Ala Gly  Ala Ala Cys 
    2735                 2740                 2745             


Cys Ala  Gly Ala Ala Gly Cys  Thr Gly Ala Thr Cys  Gly Cys Thr 
    2750                 2755                 2760             


Ala Ala  Cys Cys Ala Gly Thr  Thr Cys Ala Ala Cys  Thr Cys Cys 
    2765                 2770                 2775             


Gly Cys  Cys Ala Thr Cys Gly  Gly Cys Ala Ala Gly  Ala Thr Cys 
    2780                 2785                 2790             


Cys Ala  Gly Gly Ala Cys Ala  Gly Cys Cys Thr Gly  Ala Gly Cys 
    2795                 2800                 2805             


Thr Cys  Thr Ala Cys Thr Gly  Cys Cys Thr Cys Thr  Gly Cys Thr 
    2810                 2815                 2820             


Cys Thr  Gly Gly Gly Ala Ala  Ala Gly Cys Thr Gly  Cys Ala Gly 
    2825                 2830                 2835             


Gly Ala  Cys Gly Thr Cys Gly  Thr Gly Ala Ala Cys  Cys Ala Gly 
    2840                 2845                 2850             


Ala Ala  Cys Gly Cys Cys Cys  Ala Gly Gly Cys Thr  Cys Thr Gly 
    2855                 2860                 2865             


Ala Ala  Cys Ala Cys Cys Cys  Thr Gly Gly Thr Cys  Ala Ala Gly 
    2870                 2875                 2880             


Cys Ala  Gly Cys Thr Gly Thr  Cys Ala Thr Cys Cys  Ala Ala Cys 
    2885                 2890                 2895             


Thr Thr  Cys Gly Gly Ala Gly  Cys Cys Ala Thr Cys  Ala Gly Cys 
    2900                 2905                 2910             


Thr Cys  Thr Gly Thr Gly Cys  Thr Gly Ala Ala Cys  Gly Ala Cys 
    2915                 2920                 2925             


Ala Thr  Cys Cys Thr Cys Gly  Cys Thr Ala Gly Gly  Cys Thr Gly 
    2930                 2935                 2940             


Gly Ala  Cys Cys Cys Thr Cys  Cys Cys Gly Ala Gly  Gly Cys Thr 
    2945                 2950                 2955             


Gly Ala  Ala Gly Thr Cys Cys  Ala Gly Ala Thr Cys  Gly Ala Cys 
    2960                 2965                 2970             


Ala Gly  Gly Cys Thr Gly Ala  Thr Cys Ala Cys Thr  Gly Gly Thr 
    2975                 2980                 2985             


Ala Gly  Ala Cys Thr Gly Cys  Ala Gly Thr Cys Ala  Cys Thr Gly 
    2990                 2995                 3000             


Cys Ala  Gly Ala Cys Cys Thr  Ala Cys Gly Thr Gly  Ala Cys Thr 
    3005                 3010                 3015             


Cys Ala  Gly Cys Ala Gly Cys  Thr Gly Ala Thr Cys  Cys Gly Cys 
    3020                 3025                 3030             


Gly Cys  Cys Gly Cys Thr Gly  Ala Ala Ala Thr Cys  Cys Gly Thr 
    3035                 3040                 3045             


Gly Cys  Cys Thr Cys Cys Gly  Cys Thr Ala Ala Cys  Cys Thr Gly 
    3050                 3055                 3060             


Gly Cys  Cys Gly Cys Thr Ala  Cys Cys Ala Ala Gly  Ala Thr Gly 
    3065                 3070                 3075             


Ala Gly  Cys Gly Ala Gly Thr  Gly Cys Gly Thr Cys  Cys Thr Gly 
    3080                 3085                 3090             


Gly Gly  Ala Cys Ala Gly Thr  Cys Thr Ala Ala Gly  Ala Gly Ala 
    3095                 3100                 3105             


Gly Thr  Gly Gly Ala Cys Thr  Thr Cys Thr Gly Cys  Gly Gly Cys 
    3110                 3115                 3120             


Ala Ala  Gly Gly Gly Ala Thr  Ala Cys Cys Ala Cys  Cys Thr Gly 
    3125                 3130                 3135             


Ala Thr  Gly Thr Cys Ala Thr  Thr Cys Cys Cys Ala  Cys Ala Gly 
    3140                 3145                 3150             


Thr Cys  Cys Gly Cys Thr Cys  Cys Thr Cys Ala Cys  Gly Gly Thr 
    3155                 3160                 3165             


Gly Thr  Cys Gly Thr Gly Thr  Thr Cys Cys Thr Gly  Cys Ala Cys 
    3170                 3175                 3180             


Gly Thr  Cys Ala Cys Cys Thr  Ala Cys Gly Thr Gly  Cys Cys Cys 
    3185                 3190                 3195             


Gly Cys  Cys Cys Ala Gly Gly  Ala Gly Ala Ala Gly  Ala Ala Cys 
    3200                 3205                 3210             


Thr Thr  Cys Ala Cys Cys Ala  Cys Thr Gly Cys Cys  Cys Cys Ala 
    3215                 3220                 3225             


Gly Cys  Thr Ala Thr Cys Thr  Gly Cys Cys Ala Cys  Gly Ala Cys 
    3230                 3235                 3240             


Gly Gly  Cys Ala Ala Gly Gly  Cys Thr Cys Ala Cys  Thr Thr Cys 
    3245                 3250                 3255             


Cys Cys  Cys Cys Gly Cys Gly  Ala Ala Gly Gly Thr  Gly Thr Cys 
    3260                 3265                 3270             


Thr Thr  Cys Gly Thr Gly Thr  Cys Ala Ala Ala Cys  Gly Gly Cys 
    3275                 3280                 3285             


Ala Cys  Cys Cys Ala Cys Thr  Gly Gly Thr Thr Cys  Gly Thr Cys 
    3290                 3295                 3300             


Ala Cys  Thr Cys Ala Gly Cys  Gly Thr Ala Ala Cys  Thr Thr Cys 
    3305                 3310                 3315             


Thr Ala  Cys Gly Ala Gly Cys  Cys Ala Cys Ala Gly  Ala Thr Cys 
    3320                 3325                 3330             


Ala Thr  Cys Ala Cys Cys Ala  Cys Thr Cys Ala Cys  Ala Ala Cys 
    3335                 3340                 3345             


Ala Cys  Thr Thr Thr Cys Gly  Thr Gly Thr Cys Cys  Gly Gly Ala 
    3350                 3355                 3360             


Ala Ala  Cys Thr Gly Cys Gly  Ala Cys Gly Thr Gly  Gly Thr Cys 
    3365                 3370                 3375             


Ala Thr  Cys Gly Gly Thr Ala  Thr Cys Gly Thr Cys  Ala Ala Cys 
    3380                 3385                 3390             


Ala Ala  Cys Ala Cys Cys Gly  Thr Gly Thr Ala Cys  Gly Ala Cys 
    3395                 3400                 3405             


Cys Cys  Thr Cys Thr Gly Cys  Ala Gly Cys Cys Cys  Gly Ala Gly 
    3410                 3415                 3420             


Cys Thr  Gly Gly Ala Cys Thr  Cys Ala Thr Thr Cys  Ala Ala Gly 
    3425                 3430                 3435             


Gly Ala  Ala Gly Ala Gly Cys  Thr Gly Gly Ala Cys  Ala Ala Gly 
    3440                 3445                 3450             


Thr Ala  Cys Thr Thr Cys Ala  Ala Gly Ala Ala Cys  Cys Ala Cys 
    3455                 3460                 3465             


Ala Cys  Cys Thr Cys Cys Cys  Cys Thr Gly Ala Cys  Gly Thr Cys 
    3470                 3475                 3480             


Gly Ala  Cys Cys Thr Gly Gly  Gly Cys Gly Ala Cys  Ala Thr Cys 
    3485                 3490                 3495             


Thr Cys  Ala Gly Gly Ala Ala  Thr Cys Ala Ala Cys  Gly Cys Thr 
    3500                 3505                 3510             


Thr Cys  Cys Gly Thr Cys Gly  Thr Gly Ala Ala Cys  Ala Thr Cys 
    3515                 3520                 3525             


Cys Ala  Gly Ala Ala Gly Gly  Ala Gly Ala Thr Cys  Gly Ala Cys 
    3530                 3535                 3540             


Cys Gly  Cys Cys Thr Gly Ala  Ala Cys Gly Ala Ala  Gly Thr Gly 
    3545                 3550                 3555             


Gly Cys  Cys Ala Ala Gly Ala  Ala Cys Cys Thr Gly  Ala Ala Cys 
    3560                 3565                 3570             


Gly Ala  Ala Thr Cys Ala Cys  Thr Gly Ala Thr Cys  Gly Ala Cys 
    3575                 3580                 3585             


Cys Thr  Gly Cys Ala Gly Gly  Ala Gly Cys Thr Gly  Gly Gly Cys 
    3590                 3595                 3600             


Ala Ala  Gly Thr Ala Cys Gly  Ala Ala Cys Ala Gly  Thr Ala Cys 
    3605                 3610                 3615             


Ala Thr  Cys Ala Ala Gly Thr  Gly Gly Cys Cys Thr  Thr Gly Gly 
    3620                 3625                 3630             


Thr Ala  Cys Ala Thr Cys Thr  Gly Gly Cys Thr Gly  Gly Gly Thr 
    3635                 3640                 3645             


Thr Thr  Cys Ala Thr Cys Gly  Cys Thr Gly Gly Cys  Cys Thr Cys 
    3650                 3655                 3660             


Ala Thr  Cys Gly Cys Thr Ala  Thr Cys Gly Thr Gly  Ala Thr Gly 
    3665                 3670                 3675             


Gly Thr  Gly Ala Cys Cys Ala  Thr Cys Ala Thr Gly  Cys Thr Gly 
    3680                 3685                 3690             


Thr Gly  Cys Thr Gly Cys Ala  Thr Gly Ala Cys Thr  Thr Cys Ala 
    3695                 3700                 3705             


Thr Gly  Cys Thr Gly Cys Thr  Cys Cys Thr Gly Cys  Cys Thr Gly 
    3710                 3715                 3720             


Ala Ala  Gly Gly Gly Cys Thr  Gly Cys Thr Gly Cys  Ala Gly Cys 
    3725                 3730                 3735             


Thr Gly  Cys Gly Gly Ala Thr  Cys Thr Thr Gly Cys  Thr Gly Cys 
    3740                 3745                 3750             


Ala Ala  Gly Thr Thr Cys Gly  Ala Cys Gly Ala Gly  Gly Ala Cys 
    3755                 3760                 3765             


Gly Ala  Cys Thr Cys Cys Gly  Ala Ala Cys Cys Cys  Gly Thr Cys 
    3770                 3775                 3780             


Cys Thr  Gly Ala Ala Gly Gly  Gly Ala Gly Thr Gly  Ala Ala Gly 
    3785                 3790                 3795             


Cys Thr  Gly Cys Ala Cys Thr  Ala Cys Ala Cys Cys  Thr Ala Ala 
    3800                 3805                 3810             


<210>  136
<211>  1270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  136

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 
65                  70                  75                  80  


Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 
                85                  90                  95      


Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 
            100                 105                 110         


Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 
        115                 120                 125             


Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr His Lys 
    130                 135                 140                 


Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala 
145                 150                 155                 160 


Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu 
                165                 170                 175     


Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys 
            180                 185                 190         


Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn 
        195                 200                 205             


Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val 
    210                 215                 220                 


Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser His Gln Gln Ala Gln Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr His  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  137
<211>  1268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  137

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 
65                  70                  75                  80  


Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 
                85                  90                  95      


Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 
            100                 105                 110         


Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 
        115                 120                 125             


Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr His Lys 
    130                 135                 140                 


Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala 
145                 150                 155                 160 


Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu 
                165                 170                 175     


Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys 
            180                 185                 190         


Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn 
        195                 200                 205             


Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val 
    210                 215                 220                 


Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser His Gly Gly Ser Val Ala Ser Gln Ser Ile Ile 
        675                 680                 685             


Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn 
    690                 695                 700                 


Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr Thr Glu 
705                 710                 715                 720 


Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr 
                725                 730                 735     


Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly 
            740                 745                 750         


Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu 
        755                 760                 765             


Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr 
    770                 775                 780                 


Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile 
785                 790                 795                 800 


Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu 
                805                 810                 815     


Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr 
            820                 825                 830         


Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln 
        835                 840                 845             


Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met 
    850                 855                 860                 


Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly 
865                 870                 875                 880 


Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln 
                885                 890                 895     


Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr 
            900                 905                 910         


Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys 
        915                 920                 925             


Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln 
    930                 935                 940                 


Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln 
945                 950                 955                 960 


Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu 
                965                 970                 975     


Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile 
            980                 985                 990         


Thr Gly Arg Leu Gln Ser Leu Gln  Thr Tyr Val Thr Gln  Gln Leu Ile 
        995                 1000                 1005             


Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys 
    1010                 1015                 1020             


Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys 
    1025                 1030                 1035             


Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro Gln Ser  Ala Pro His 
    1040                 1045                 1050             


Gly Val  Val Phe Leu His Val  Thr Tyr Val Pro Ala  Gln Glu Lys 
    1055                 1060                 1065             


Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His Asp Gly  Lys Ala His 
    1070                 1075                 1080             


Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn Gly Thr  His Trp Phe 
    1085                 1090                 1095             


Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr His 
    1100                 1105                 1110             


Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val Val Ile  Gly Ile Val 
    1115                 1120                 1125             


Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe 
    1130                 1135                 1140             


Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp 
    1145                 1150                 1155             


Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn 
    1160                 1165                 1170             


Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu 
    1175                 1180                 1185             


Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln 
    1190                 1195                 1200             


Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu Gly Phe  Ile Ala Gly 
    1205                 1210                 1215             


Leu Ile  Ala Ile Val Met Val  Thr Ile Met Leu Cys  Cys Met Thr 
    1220                 1225                 1230             


Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys Ser Cys  Gly Ser Cys 
    1235                 1240                 1245             


Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro Val Leu  Lys Gly Val 
    1250                 1255                 1260             


Lys Leu  His Tyr Thr 
    1265             


<210>  138
<211>  1255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  138

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe 
    50                  55                  60                  


Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr 
65                  70                  75                  80  


Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp 
                85                  90                  95      


Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val 
            100                 105                 110         


Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val 
        115                 120                 125             


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
    130                 135                 140                 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
145                 150                 155                 160 


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
                165                 170                 175     


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
            180                 185                 190         


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
        195                 200                 205             


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
    210                 215                 220                 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
225                 230                 235                 240 


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
                245                 250                 255     


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
            260                 265                 270         


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
        275                 280                 285             


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
    290                 295                 300                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
305                 310                 315                 320 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                325                 330                 335     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            340                 345                 350         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        355                 360                 365             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    370                 375                 380                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
385                 390                 395                 400 


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                405                 410                 415     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            420                 425                 430         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        435                 440                 445             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    450                 455                 460                 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
465                 470                 475                 480 


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                485                 490                 495     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            500                 505                 510         


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
        515                 520                 525             


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
    530                 535                 540                 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val 
545                 550                 555                 560 


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
                565                 570                 575     


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
            580                 585                 590         


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
        595                 600                 605             


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
    610                 615                 620                 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
625                 630                 635                 640 


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
                645                 650                 655     


Ser Tyr Gln Thr Gln Thr Asn Ser His Gly Gly Ser Val Ala Ser Gln 
            660                 665                 670         


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala 
        675                 680                 685             


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val 
    690                 695                 700                 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
705                 710                 715                 720 


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
                725                 730                 735     


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
            740                 745                 750         


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
        755                 760                 765             


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
    770                 775                 780                 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
785                 790                 795                 800 


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
                805                 810                 815     


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
            820                 825                 830         


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
        835                 840                 845             


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
    850                 855                 860                 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
865                 870                 875                 880 


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
                885                 890                 895     


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
            900                 905                 910         


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
        915                 920                 925             


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
    930                 935                 940                 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
945                 950                 955                 960 


Asp Ile Leu Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
                965                 970                 975     


Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln 
            980                 985                 990         


Gln Leu Ile Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
        995                 1000                 1005             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1010                 1015                 1020             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1025                 1030                 1035             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1040                 1045                 1050             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1055                 1060                 1065             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1070                 1075                 1080             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1085                 1090                 1095             


Thr His  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1100                 1105                 1110             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1115                 1120                 1125             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1130                 1135                 1140             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1145                 1150                 1155             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1160                 1165                 1170             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1175                 1180                 1185             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1190                 1195                 1200             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1205                 1210                 1215             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1220                 1225                 1230             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1235                 1240                 1245             


Gly Val  Lys Leu His Tyr Thr  
    1250                 1255 


<210>  139
<211>  3807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  139
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac agcttcacta ggggtgtcta ctaccctgac      120

aaggtcttcc gctcctcagt gctgcactct actcaggacc tgttcctgcc cttcttctca      180

aacgtgacct ggttccacgc catctccggc accaacggca ctaagaggtt cgacaacccc      240

gtgctgccat tcaacgacgg cgtctacttc gcttcaaccg agaagtccaa catcatcaga      300

ggatggatct tcggcaccac tctggacagc aagactcagt ctctgctgat cgtcaacaac      360

gccaccaacg tggtcatcaa ggtgtgcgaa ttccagttct gcaacgaccc attcctgggt      420

gtgtaccaca agaacaacaa gtcatggatg gagtccgaat tccgcgtcta ctccagcgct      480

aacaactgca ctttcgagta cgtgagccag cctttcctga tggacctgga aggaaagcag      540

ggtaacttca agaacctgcg cgagttcgtg ttcaagaaca tcgacggata cttcaagatt      600

tacagcaagc acacccccat caacctggtc cgtgacctgc cacagggttt ctctgctctg      660

gagcccctgg tggacctgcc aatcggcatc aacatcacca ggttccagac tctgctggct      720

ctgcaccgct cctacctgac tccaggtgac tcatcttctg gatggactgc tggagctgct      780

gcttactacg tgggttacct gcagcctcgc accttcctgc tgaagtacaa cgaaaacggc      840

accatcactg acgccgtcga ctgcgctctg gaccctctgt cagaaaccaa gtgcactctg      900

aagtccttca ccgtggagaa gggtatctac cagactagca acttccgcgt gcagcccacc      960

gaatctatcg tccgtttccc aaacatcact aacctgtgcc ctttcggcga ggtcttcaac     1020

gccacccgct tcgcttccgt gtacgcctgg aaccgcaagc gtatctcaaa ctgcgtcgct     1080

gactactccg tgctgtacaa ctcagcctcc ttcagcacct tcaagtgcta cggtgtgtca     1140

cccactaagc tgaacgacct gtgcttcacc aacgtctacg ccgactcctt cgtgatccgc     1200

ggcgacgagg tccgtcagat cgctccaggc cagactggaa agatcgccga ctacaactac     1260

aagctgcctg acgacttcac cggctgcgtc atcgcttgga acagcaacaa cctggactct     1320

aaagtgggtg gcaactacaa ctacctgtac aggctgttca gaaagagcaa cctgaagccc     1380

ttcgagcgcg acatcagcac tgaaatctac caggctggct ctaccccatg caacggtgtc     1440

gagggcttca actgctactt ccctctgcag tcttacggat tccagcccac ttacggtgtg     1500

ggctaccagc cataccgtgt ggtcgtgctg agcttcgaac tgctgcacgc ccctgctact     1560

gtgtgcggac ccaagaagtc taccaacctg gtcaagaaca agtgcgtgaa cttcaacttc     1620

aacggtctga ccggaactgg tgtcctgacc gagagcaaca agaagttcct gcccttccag     1680

cagttcggtc gcgacatcga cgacaccact gacgccgtgc gtgaccctca gaccctggaa     1740

atcctggaca tcactccctg ctcattcgga ggtgtctccg tgatcactcc aggaaccaac     1800

acttctaacc aggtcgctgt gctgtaccag ggtgtcaact gcaccgaggt cccagtggcc     1860

atccacgctg accagctgac ccctacttgg agggtgtact ccaccggcag caacgtgttc     1920

cagactagag ctggttgcct gatcggcgcc gagcacgtga acaacagcta cgaatgcgac     1980

atcccaatcg gcgctggaat ctgcgcctct taccagaccc agactaacag ccacggtggt     2040

tctgtcgcct ctcagtcaat catcgcttac actatgtcac tgggcgctga aaactccgtg     2100

gcctactcta acaactcaat cgccatccct atcaacttca ccatctcagt gaccactgag     2160

atcctgcccg tcagcatgac caagacttct gtggactgca ctatgtacat ctgcggagac     2220

agcaccgaat gctctaacct gctgctgcag tacggctcct tctgcaccca gctgaaccgc     2280

gctctgactg gaatcgccgt ggagcaggac aagaacactc aggaagtctt cgctcaggtg     2340

aagcaaatct acaagacccc acctatcaag gacttcggcg gattcaactt ctcccagatc     2400

ctgccagacc cttccaagcc aagcaagagg tctttcatcg aggacctgct gttcaacaag     2460

gtcaccctgg ccgacgctgg cttcatcaag cagtacggag actgcctggg tgacatcgcc     2520

gctcgcgacc tgatctgcgc tcagaagttc aacggactga ccgtgctgcc cccactgctg     2580

actgacgaaa tgatcgccca gtacacttca gccctgctgg ctggaaccat cacttctggt     2640

tggaccttcg gtgctggtgc tgctctgcag atccctttcg ctatgcagat ggcctaccgt     2700

ttcaacggaa tcggtgtcac ccagaacgtg ctgtacgaga accagaagct gatcgctaac     2760

cagttcaact ccgccatcgg caagatccag gacagcctga gctctactgc ctctgctctg     2820

ggaaagctgc aggacgtcgt gaaccagaac gcccaggctc tgaacaccct ggtcaagcag     2880

ctgtcatcca acttcggagc catcagctct gtgctgaacg acatcctcgc taggctggac     2940

cctcccgagg ctgaagtcca gatcgacagg ctgatcactg gtagactgca gtcactgcag     3000

acctacgtga ctcagcagct gatccgcgcc gctgaaatcc gtgcctccgc taacctggcc     3060

gctaccaaga tgagcgagtg cgtcctggga cagtctaaga gagtggactt ctgcggcaag     3120

ggataccacc tgatgtcatt cccacagtcc gctcctcacg gtgtcgtgtt cctgcacgtc     3180

acctacgtgc ccgcccagga gaagaacttc accactgccc cagctatctg ccacgacggc     3240

aaggctcact tcccccgcga aggtgtcttc gtgtcaaacg gcacccactg gttcgtcact     3300

cagcgtaact tctacgagcc acagatcatc accactcaca acactttcgt gtccggaaac     3360

tgcgacgtgg tcatcggtat cgtcaacaac accgtgtacg accctctgca gcccgagctg     3420

gactcattca aggaagagct ggacaagtac ttcaagaacc acacctcccc tgacgtcgac     3480

ctgggcgaca tctcaggaat caacgcttcc gtcgtgaaca tccagaagga gatcgaccgc     3540

ctgaacgaag tggccaagaa cctgaacgaa tcactgatcg acctgcagga gctgggcaag     3600

tacgaacagt acatcaagtg gccttggtac atctggctgg gtttcatcgc tggcctcatc     3660

gctatcgtga tggtgaccat catgctgtgc tgcatgactt catgctgctc ctgcctgaag     3720

ggctgctgca gctgcggatc ttgctgcaag ttcgacgagg acgactccga acccgtcctg     3780

aagggagtga agctgcacta cacctaa                                         3807


<210>  140
<211>  1270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  140

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Gly Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  141
<211>  1257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  141

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Ala Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu His Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Gly Ser Val 
            660                 665                 670         


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn 
        675                 680                 685             


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
    690                 695                 700                 


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
705                 710                 715                 720 


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
                725                 730                 735     


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
            740                 745                 750         


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
        755                 760                 765             


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
    770                 775                 780                 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
785                 790                 795                 800 


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
                805                 810                 815     


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
            820                 825                 830         


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
        835                 840                 845             


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
    850                 855                 860                 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
865                 870                 875                 880 


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
                885                 890                 895     


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
            900                 905                 910         


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
        915                 920                 925             


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
    930                 935                 940                 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
945                 950                 955                 960 


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
                965                 970                 975     


Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
            980                 985                 990         


Thr Gln Gln Leu Ile Arg Ala Ala  Glu Ile Arg Ala Ser  Ala Asn Leu 
        995                 1000                 1005             


Ala Ala  Thr Lys Met Ser Glu  Cys Val Leu Gly Gln  Ser Lys Arg 
    1010                 1015                 1020             


Val Asp  Phe Cys Gly Lys Gly  Tyr His Leu Met Ser  Phe Pro Gln 
    1025                 1030                 1035             


Ser Ala  Pro His Gly Val Val  Phe Leu His Val Thr  Tyr Val Pro 
    1040                 1045                 1050             


Ala Gln  Glu Lys Asn Phe Thr  Thr Ala Pro Ala Ile  Cys His Asp 
    1055                 1060                 1065             


Gly Lys  Ala His Phe Pro Arg  Glu Gly Val Phe Val  Ser Asn Gly 
    1070                 1075                 1080             


Thr His  Trp Phe Val Thr Gln  Arg Asn Phe Tyr Glu  Pro Gln Ile 
    1085                 1090                 1095             


Ile Thr  Thr Asp Asn Thr Phe  Val Ser Gly Asn Cys  Asp Val Val 
    1100                 1105                 1110             


Ile Gly  Ile Val Asn Asn Thr  Val Tyr Asp Pro Leu  Gln Pro Glu 
    1115                 1120                 1125             


Leu Asp  Ser Phe Lys Glu Glu  Leu Asp Lys Tyr Phe  Lys Asn His 
    1130                 1135                 1140             


Thr Ser  Pro Asp Val Asp Leu  Gly Asp Ile Ser Gly  Ile Asn Ala 
    1145                 1150                 1155             


Ser Val  Val Asn Ile Gln Lys  Glu Ile Asp Arg Leu  Asn Glu Val 
    1160                 1165                 1170             


Ala Lys  Asn Leu Asn Glu Ser  Leu Ile Asp Leu Gln  Glu Leu Gly 
    1175                 1180                 1185             


Lys Tyr  Glu Gln Tyr Ile Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly 
    1190                 1195                 1200             


Phe Ile  Ala Gly Leu Ile Ala  Ile Val Met Val Thr  Ile Met Leu 
    1205                 1210                 1215             


Cys Cys  Met Thr Ser Cys Cys  Ser Cys Leu Lys Gly  Cys Cys Ser 
    1220                 1225                 1230             


Cys Gly  Ser Cys Cys Lys Phe  Asp Glu Asp Asp Ser  Glu Pro Val 
    1235                 1240                 1245             


Leu Lys  Gly Val Lys Leu His  Tyr Thr 
    1250                 1255         


<210>  142
<211>  3816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  142
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cctgaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcacta ggggtgtgta ctacccagac      120

aaggtcttcc gctcctcagt gctgcactca actcaggacc tgttcctgcc tttcttctcc      180

aacgtcacct ggttccacgc tatccacgtg tccggcacca acggcactaa gaggttcgcc      240

aaccccgtgc tgccattcaa cgacggagtc tacttcgctt caaccgagaa gtccaacatc      300

atcagaggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcta ccaacgtggt catcaaggtg tgcgaattcc agttctgtaa cgaccccttc      420

ctgggtgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagattt acagcaagca cacccccatc aacctggtcc gtggcctgcc acagggattc      660

tctgctctgg agcccctggt ggacctgcca atcggcatca acatcaccag gttccagact      720

ctgcacgccc tgcacagatc ctacctgact cccggagact cttcatccgg ttggaccgct      780

ggcgctgccg cttactacgt gggttacctg cagccacgca ccttcctgct gaagtacaac      840

gaaaacggca ccatcactga cgccgtcgac tgcgctctgg acccactgtc agaaaccaag      900

tgcactctga agtccttcac cgtggagaag ggaatctacc agacttcaaa cttccgcgtg      960

cagcctaccg aatccatcgt ccgtttccca aacatcacta acctgtgccc tttcggagag     1020

gtcttcaacg ccacccgctt cgcttccgtg tacgcctgga accgcaagcg tatctcaaac     1080

tgcgtcgctg actactccgt gctgtacaac tcagcctcct tcagcacctt caagtgctac     1140

ggagtgtcac ccactaagct gaacgacctg tgcttcacca acgtctacgc tgactccttc     1200

gtgatccgcg gtgacgaggt ccgtcagatc gctccaggcc agactggaaa catcgccgac     1260

tacaactaca agctgcctga cgacttcacc ggttgcgtca tcgcctggaa cagcaacaac     1320

ctggactcta aagtgggtgg caactacaac tacctgtaca ggctgttcag aaagtcaaac     1380

ctgaagccct tcgagcgcga catcagcact gaaatctacc aggctggttc taccccatgc     1440

aacggtgtca agggcttcaa ctgctacttc cctctgcagt cctacggctt ccagcccact     1500

tacggtgtgg gctaccagcc ataccgtgtg gtcgtgctga gcttcgagct gctgcacgcc     1560

cctgctactg tgtgcggacc caagaagtct accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggtctgac cggaactggt gtcctgaccg agagcaacaa gaagttcctg     1680

cctttccagc agttcggtcg cgacatcgct gacaccactg acgccgtgcg tgaccctcag     1740

accctggaaa tcctggacat cactccctgc tcattcggag gtgtctccgt gatcactccc     1800

ggaaccaaca cttctaacca ggtcgctgtg ctgtaccagg gtgtgaactg caccgaagtc     1860

ccagtggcca tccacgctga ccagctgacc cctacttgga gggtgtactc caccggcagc     1920

aacgtcttcc agactagagc tggatgcctg atcggtgccg agcacgtcaa caacagctac     1980

gaatgcgaca tccctatcgg cgctggaatc tgcgcctctt accagaccca gactaacagc     2040

ccaggtggtt ctgtggcctc tcagtcaatc atcgcctaca ccatgtcact gggtgtcgaa     2100

aactccgtgg cttactctaa caactcaatc gccatcccta ccaacttcac tatcagcgtg     2160

accactgaga tcctgcccgt cagcatgacc aagacttctg tggactgcac tatgtacatc     2220

tgcggcgaca gcaccgaatg ctctaacctg ctgctgcagt acggttcttt ctgcacccag     2280

ctgaaccgcg ctctgactgg catcgccgtg gagcaggaca agaacactca ggaagtcttc     2340

gctcaggtga agcaaatcta caagacccca cctatcaagg acttcggcgg attcaacttc     2400

tcccagatcc tgccagaccc ttccaagccc agcaagaggt ctttcatcga ggacctgctg     2460

ttcaacaagg tcactctggc cgacgctggt ttcatcaagc agtacggcga ctgcctggga     2520

gacatcgctg ctcgcgacct gatctgcgcc cagaagttca acggcctgac cgtgctgccc     2580

ccactgctga ctgacgaaat gatcgctcag tacactagcg ccctgctggc tggcaccatc     2640

acttcaggtt ggactttcgg tgctggtgct gctctgcaga tcccattcgc tatgcagatg     2700

gcctaccgtt tcaacggaat cggtgtcacc cagaacgtgc tgtacgagaa ccagaagctg     2760

atcgctaacc agttcaactc agccatcggc aagatccagg acagcctgag ctctactgcc     2820

tctgctctgg gaaagctgca ggacgtcgtg aaccagaacg cccaggctct gaacaccctg     2880

gtcaagcagc tgtcatccaa cttcggcgcc atcagctctg tgctgaacga catcctgtcc     2940

aggctggacc ctcccgaggc tgaagtccag atcgacaggc tgatcactgg aagactgcag     3000

tcactgcaga cctacgtgac tcagcagctg atccgcgccg ctgaaatccg tgcctccgct     3060

aacctggccg ctaccaagat gagcgagtgc gtcctgggcc agtctaagag agtggacttc     3120

tgcggcaagg gataccacct gatgtcattc ccacagtccg cccctcacgg agtcgtgttc     3180

ctgcacgtca cctacgtgcc cgctcaggag aagaacttca ccactgcccc agctatctgc     3240

cacgacggca aggctcactt ccctcgcgaa ggagtcttcg tgagcaacgg cacccactgg     3300

ttcgtcactc agcgtaactt ctacgagccc cagatcatca ccactgacaa cactttcgtg     3360

tctggcaact gcgacgtggt catcggtatc gtcaacaaca ccgtgtacga ccctctgcag     3420

cccgagctgg actcattcaa ggaagagctg gacaagtact tcaagaacca cacctcccct     3480

gacgtcgacc tgggtgacat ctcaggcatc aacgcctccg tcgtgaacat ccagaaggag     3540

atcgaccgcc tgaacgaagt ggctaagaac ctgaacgaaa gcctgatcga cctgcaggag     3600

ctgggcaagt acgaacagta catcaagtgg ccatggtaca tctggctggg attcatcgct     3660

ggcctcatcg ctatcgtgat ggtgaccatc atgctgtgct gcatgacttc atgctgctcc     3720

tgcctgaagg gttgctgcag ctgcggctct tgctgcaagt tcgacgagga cgactctgaa     3780

cctgtcctga agggagtgaa gctgcactac acctaa                               3816


<210>  143
<211>  1269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  143

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn Phe Ser Gln 
785                 790                 795                 800 


Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp 
                805                 810                 815     


Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln 
            820                 825                 830         


Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala 
        835                 840                 845             


Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu 
    850                 855                 860                 


Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser 
865                 870                 875                 880 


Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met 
                885                 890                 895     


Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu 
            900                 905                 910         


Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly 
        915                 920                 925             


Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu 
    930                 935                 940                 


Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys 
945                 950                 955                 960 


Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile 
                965                 970                 975     


Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu 
            980                 985                 990         


Ile Thr Gly Arg Leu Gln Ser Leu  Gln Thr Tyr Val Thr  Gln Gln Leu 
        995                 1000                 1005             


Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala Asn Leu  Ala Ala Thr 
    1010                 1015                 1020             


Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser Lys Arg  Val Asp Phe 
    1025                 1030                 1035             


Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe Pro Gln  Ser Ala Pro 
    1040                 1045                 1050             


His Gly  Val Val Phe Leu His  Val Thr Tyr Val Pro  Ala Gln Glu 
    1055                 1060                 1065             


Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys His Asp  Gly Lys Ala 
    1070                 1075                 1080             


His Phe  Pro Arg Glu Gly Val  Phe Val Ser Asn Gly  Thr His Trp 
    1085                 1090                 1095             


Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro Gln Ile  Ile Thr Thr 
    1100                 1105                 1110             


Asp Asn  Thr Phe Val Ser Gly  Asn Cys Asp Val Val  Ile Gly Ile 
    1115                 1120                 1125             


Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln Pro Glu  Leu Asp Ser 
    1130                 1135                 1140             


Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys Asn His  Thr Ser Pro 
    1145                 1150                 1155             


Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile Asn Ala  Ser Val Val 
    1160                 1165                 1170             


Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn Glu Val  Ala Lys Asn 
    1175                 1180                 1185             


Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu Leu Gly  Lys Tyr Glu 
    1190                 1195                 1200             


Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp Leu Gly  Phe Ile Ala 
    1205                 1210                 1215             


Gly Leu  Ile Ala Ile Val Met  Val Thr Ile Met Leu  Cys Cys Met 
    1220                 1225                 1230             


Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys Cys Ser  Cys Gly Ser 
    1235                 1240                 1245             


Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu Pro Val  Leu Lys Gly 
    1250                 1255                 1260             


Val Lys  Leu His Tyr Thr 
    1265                 


<210>  144
<211>  1256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  144

Gln Cys Val Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Ala Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
225                 230                 235                 240 


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
                245                 250                 255     


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
            260                 265                 270         


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
        275                 280                 285             


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
    290                 295                 300                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
305                 310                 315                 320 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                325                 330                 335     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            340                 345                 350         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        355                 360                 365             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    370                 375                 380                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
385                 390                 395                 400 


Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                405                 410                 415     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            420                 425                 430         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        435                 440                 445             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    450                 455                 460                 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
465                 470                 475                 480 


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                485                 490                 495     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            500                 505                 510         


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
        515                 520                 525             


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
    530                 535                 540                 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
545                 550                 555                 560 


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
                565                 570                 575     


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
            580                 585                 590         


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
        595                 600                 605             


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
    610                 615                 620                 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
625                 630                 635                 640 


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
                645                 650                 655     


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
            660                 665                 670         


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser 
        675                 680                 685             


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
    690                 695                 700                 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
705                 710                 715                 720 


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
                725                 730                 735     


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
            740                 745                 750         


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
        755                 760                 765             


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn 
    770                 775                 780                 


Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe 
785                 790                 795                 800 


Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 
                805                 810                 815     


Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu 
            820                 825                 830         


Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 
        835                 840                 845             


Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr 
    850                 855                 860                 


Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 
865                 870                 875                 880 


Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 
                885                 890                 895     


Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser 
            900                 905                 910         


Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 
        915                 920                 925             


Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 
    930                 935                 940                 


Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 
945                 950                 955                 960 


Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile 
                965                 970                 975     


Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 
            980                 985                 990         


Gln Gln Leu Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
        995                 1000                 1005             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1010                 1015                 1020             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1025                 1030                 1035             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1040                 1045                 1050             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1055                 1060                 1065             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1070                 1075                 1080             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1085                 1090                 1095             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1100                 1105                 1110             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1115                 1120                 1125             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1130                 1135                 1140             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1145                 1150                 1155             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1160                 1165                 1170             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1175                 1180                 1185             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1190                 1195                 1200             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1205                 1210                 1215             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1220                 1225                 1230             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1235                 1240                 1245             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1250                 1255     


<210>  145
<211>  3812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  145
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cttcaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcacta ggggtgtgta ctacccagac      120

aaggtcttcc gctcctcagt gctgcactca actcaggacc tgttcctgcc tttcttctcc      180

aacgtcacct ggttccacgc tatccacgtg tccggcacca acggcactaa gaggttcgcc      240

aaccccgtgc tgccattcaa cgacggagtc tacttcgctt caaccgagaa gtccaacatc      300

atcagaggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcta ccaacgtggt catcaaggtg tgcgaattcc agttctgtaa cgaccccttc      420

ctgggtgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagattt acagcaagca cacccccatc aacctggtcc gtggcctgcc acagggattc      660

tctgctctgg agcccctggt ggacctgcca atcggcatca acatcaccag gttccagact      720

ctgcacagat cctacctgac tcccggagac tcttcatccg gttggaccgc tggcgctgcc      780

gcttactacg tgggttacct gcagccacgc accttcctgc tgaagtacaa cgaaaacggc      840

accatcactg acgccgtcga ctgcgctctg gacccactgt cagaaaccaa gtgcactctg      900

aagtccttca ccgtggagaa gggaatctac cagacttcaa acttccgcgt gcagcctacc      960

gaatccatcg tccgtttccc aaacatcact aacctgtgcc ctttcggaga ggtcttcaac     1020

gccacccgct tcgcttccgt gtacgcctgg aaccgcaagc gtatctcaaa ctgcgtcgct     1080

gactactccg tgctgtacaa ctcagcctcc ttcagcacct tcaagtgcta cggagtgtca     1140

cccactaagc tgaacgacct gtgcttcacc aacgtctacg ctgactcctt cgtgatccgc     1200

ggtgacgagg tccgtcagat cgctccaggc cagactggaa acatcgccga ctacaactac     1260

aagctgcctg acgacttcac cggttgcgtc atcgcctgga acagcaacaa cctggactct     1320

aaagtgggtg gcaactacaa ctacctgtac aggctgttca gaaagtcaaa cctgaagccc     1380

ttcgagcgcg acatcagcac tgaaatctac caggctggtt ctaccccatg caacggtgtc     1440

aagggcttca actgctactt ccctctgcag tcctacggct tccagcccac ttacggtgtg     1500

ggctaccagc cataccgtgt ggtcgtgctg agcttcgagc tgctgcacgc ccctgctact     1560

gtgtgcggac ccaagaagtc taccaacctg gtcaagaaca agtgcgtgaa cttcaacttc     1620

aacggtctga ccggaactgg tgtcctgacc gagagcaaca agaagttcct gcctttccag     1680

cagttcggtc gcgacatcgc tgacaccact gacgccgtgc gtgaccctca gaccctggaa     1740

atcctggaca tcactccctg ctcattcgga ggtgtctccg tgatcactcc cggaaccaac     1800

acttctaacc aggtcgctgt gctgtaccag ggtgtgaact gcaccgaagt cccagtggcc     1860

atccacgctg accagctgac ccctacttgg agggtgtact ccaccggcag caacgtcttc     1920

cagactagag ctggatgcct gatcggtgcc gagcacgtca acaacagcta cgaatgcgac     1980

atccctatcg gcgctggaat ctgcgcctct taccagaccc agactaacag cccccagcag     2040

gctcagtctg tggcctctca gtcaatcatc gcctacacca tgtcactggg tgtcgaaaac     2100

tccgtggctt actctaacaa ctcaatcgcc atccctacca acttcactat cagcgtgacc     2160

actgagatcc tgcccgtcag catgaccaag acttctgtgg actgcactat gtacatctgc     2220

ggcgacagca ccgaatgctc taacctgctg ctgcagtacg gttctttctg cacccagctg     2280

aaccgcgctc tgactggcat cgccgtggag caggacaaga acactcagga agtcttcgct     2340

caggtgaagc aaatctacaa gaccccacct atcaaggact tcggcggatt caacttctcc     2400

cagatcctgc cagacccttc caagcccagc aagaggtctt tcatcgagga cctgctgttc     2460

aacaaggtca ctctggccga cgctggtttc atcaagcagt acggcgactg cctgggagac     2520

atcgctgctc gcgacctgat ctgcgcccag aagttcaacg gcctgaccgt gctgccccca     2580

ctgctgactg acgaaatgat cgctcagtac actagcgccc tgctggctgg caccatcact     2640

tcaggttgga ctttcggtgc tggtgctgct ctgcagatcc cattcgctat gcagatggcc     2700

taccgtttca acggaatcgg tgtcacccag aacgtgctgt acgagaacca gaagctgatc     2760

gctaaccagt tcaactcagc catcggcaag atccaggaca gcctgagctc tactgcctct     2820

gctctgggaa agctgcagga cgtcgtgaac cagaacgccc aggctctgaa caccctggtc     2880

aagcagctgt catccaactt cggcgccatc agctctgtgc tgaacgacat cctgtccagg     2940

ctggaccctc ccgaggctga agtccagatc gacaggctga tcactggaag actgcagtca     3000

ctgcagacct acgtgactca gcagctgatc cgcgccgctg aaatccgtgc ctccgctaac     3060

ctggccgcta ccaagatgag cgagtgcgtc ctgggccagt ctaagagagt ggacttctgc     3120

ggcaagggat accacctgat gtcattccca cagtccgccc ctcacggagt cgtgttcctg     3180

cacgtcacct acgtgcccgc tcaggagaag aacttcacca ctgccccagc tatctgccac     3240

gacggcaagg ctcacttccc tcgcgaagga gtcttcgtga gcaacggcac ccactggttc     3300

gtcactcagc gtaacttcta cgagccccag atcatcacca ctgacaacac tttcgtgtct     3360

ggcaactgcg acgtggtcat cggtatcgtc aacaacaccg tgtacgaccc tctgcagccc     3420

gagctggact cattcaagga agagctggac aagtacttca agaaccacac ctcccctgac     3480

gtcgacctgg gtgacatctc aggcatcaac gcctccgtcg tgaacatcca gaaggagatc     3540

gaccgcctga acgaagtggc taagaacctg aacgaaagcc tgatcgacct gcaggagctg     3600

ggcaagtacg aacagtacat caagtggcca tggtacatct ggctgggatt catcgctggc     3660

ctcatcgcta tcgtgatggt gaccatcatg ctgtgctgca tgacttcatg ctgctcctgc     3720

ctgaagggtt gctgcagctg cggctcttgc tgcaagttcg acgaggacga ctctgaacct     3780

tcctgaaggg agtgaagctg cactacacct aa                                   3812


<210>  146
<211>  1267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  146

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser Pro Gly Gly Ser Val Ala Ser Gln Ser Ile Ile 
        675                 680                 685             


Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala Tyr Ser Asn 
    690                 695                 700                 


Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu 
705                 710                 715                 720 


Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr 
                725                 730                 735     


Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly 
            740                 745                 750         


Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu 
        755                 760                 765             


Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr 
    770                 775                 780                 


Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn Phe Ser Gln Ile Leu 
785                 790                 795                 800 


Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu 
                805                 810                 815     


Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly 
            820                 825                 830         


Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys 
        835                 840                 845             


Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile 
    850                 855                 860                 


Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp 
865                 870                 875                 880 


Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met 
                885                 890                 895     


Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu 
            900                 905                 910         


Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile 
        915                 920                 925             


Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp 
    930                 935                 940                 


Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu 
945                 950                 955                 960 


Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser 
                965                 970                 975     


Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr 
            980                 985                 990         


Gly Arg Leu Gln Ser Leu Gln Thr  Tyr Val Thr Gln Gln  Leu Ile Arg 
        995                 1000                 1005             


Ala Ala  Glu Ile Arg Ala Ser  Ala Asn Leu Ala Ala  Thr Lys Met 
    1010                 1015                 1020             


Ser Glu  Cys Val Leu Gly Gln  Ser Lys Arg Val Asp  Phe Cys Gly 
    1025                 1030                 1035             


Lys Gly  Tyr His Leu Met Ser  Phe Pro Gln Ser Ala  Pro His Gly 
    1040                 1045                 1050             


Val Val  Phe Leu His Val Thr  Tyr Val Pro Ala Gln  Glu Lys Asn 
    1055                 1060                 1065             


Phe Thr  Thr Ala Pro Ala Ile  Cys His Asp Gly Lys  Ala His Phe 
    1070                 1075                 1080             


Pro Arg  Glu Gly Val Phe Val  Ser Asn Gly Thr His  Trp Phe Val 
    1085                 1090                 1095             


Thr Gln  Arg Asn Phe Tyr Glu  Pro Gln Ile Ile Thr  Thr Asp Asn 
    1100                 1105                 1110             


Thr Phe  Val Ser Gly Asn Cys  Asp Val Val Ile Gly  Ile Val Asn 
    1115                 1120                 1125             


Asn Thr  Val Tyr Asp Pro Leu  Gln Pro Glu Leu Asp  Ser Phe Lys 
    1130                 1135                 1140             


Glu Glu  Leu Asp Lys Tyr Phe  Lys Asn His Thr Ser  Pro Asp Val 
    1145                 1150                 1155             


Asp Leu  Gly Asp Ile Ser Gly  Ile Asn Ala Ser Val  Val Asn Ile 
    1160                 1165                 1170             


Gln Lys  Glu Ile Asp Arg Leu  Asn Glu Val Ala Lys  Asn Leu Asn 
    1175                 1180                 1185             


Glu Ser  Leu Ile Asp Leu Gln  Glu Leu Gly Lys Tyr  Glu Gln Tyr 
    1190                 1195                 1200             


Ile Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly Phe Ile  Ala Gly Leu 
    1205                 1210                 1215             


Ile Ala  Ile Val Met Val Thr  Ile Met Leu Cys Cys  Met Thr Ser 
    1220                 1225                 1230             


Cys Cys  Ser Cys Leu Lys Gly  Cys Cys Ser Cys Gly  Ser Cys Cys 
    1235                 1240                 1245             


Lys Phe  Asp Glu Asp Asp Ser  Glu Pro Val Leu Lys  Gly Val Lys 
    1250                 1255                 1260             


Leu His  Tyr Thr 
    1265         


<210>  147
<211>  1254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  147

Gln Cys Val Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Ala Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
225                 230                 235                 240 


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
                245                 250                 255     


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
            260                 265                 270         


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
        275                 280                 285             


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
    290                 295                 300                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
305                 310                 315                 320 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                325                 330                 335     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            340                 345                 350         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        355                 360                 365             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    370                 375                 380                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
385                 390                 395                 400 


Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                405                 410                 415     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            420                 425                 430         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        435                 440                 445             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    450                 455                 460                 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
465                 470                 475                 480 


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                485                 490                 495     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            500                 505                 510         


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
        515                 520                 525             


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
    530                 535                 540                 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
545                 550                 555                 560 


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
                565                 570                 575     


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
            580                 585                 590         


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
        595                 600                 605             


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
    610                 615                 620                 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
625                 630                 635                 640 


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
                645                 650                 655     


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Gly Ser Val Ala Ser Gln 
            660                 665                 670         


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala 
        675                 680                 685             


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
    690                 695                 700                 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
705                 710                 715                 720 


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
                725                 730                 735     


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
            740                 745                 750         


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
        755                 760                 765             


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Asn Phe Ser 
    770                 775                 780                 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
785                 790                 795                 800 


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
                805                 810                 815     


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
            820                 825                 830         


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
        835                 840                 845             


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
    850                 855                 860                 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
865                 870                 875                 880 


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
                885                 890                 895     


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
            900                 905                 910         


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
        915                 920                 925             


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
    930                 935                 940                 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
945                 950                 955                 960 


Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg 
                965                 970                 975     


Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln 
            980                 985                 990         


Leu Ile Arg Ala Ala Glu Ile Arg  Ala Ser Ala Asn Leu  Ala Ala Thr 
        995                 1000                 1005             


Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser Lys Arg  Val Asp Phe 
    1010                 1015                 1020             


Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe Pro Gln  Ser Ala Pro 
    1025                 1030                 1035             


His Gly  Val Val Phe Leu His  Val Thr Tyr Val Pro  Ala Gln Glu 
    1040                 1045                 1050             


Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys His Asp  Gly Lys Ala 
    1055                 1060                 1065             


His Phe  Pro Arg Glu Gly Val  Phe Val Ser Asn Gly  Thr His Trp 
    1070                 1075                 1080             


Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro Gln Ile  Ile Thr Thr 
    1085                 1090                 1095             


Asp Asn  Thr Phe Val Ser Gly  Asn Cys Asp Val Val  Ile Gly Ile 
    1100                 1105                 1110             


Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln Pro Glu  Leu Asp Ser 
    1115                 1120                 1125             


Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys Asn His  Thr Ser Pro 
    1130                 1135                 1140             


Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile Asn Ala  Ser Val Val 
    1145                 1150                 1155             


Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn Glu Val  Ala Lys Asn 
    1160                 1165                 1170             


Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu Leu Gly  Lys Tyr Glu 
    1175                 1180                 1185             


Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp Leu Gly  Phe Ile Ala 
    1190                 1195                 1200             


Gly Leu  Ile Ala Ile Val Met  Val Thr Ile Met Leu  Cys Cys Met 
    1205                 1210                 1215             


Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys Cys Ser  Cys Gly Ser 
    1220                 1225                 1230             


Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu Pro Val  Leu Lys Gly 
    1235                 1240                 1245             


Val Lys  Leu His Tyr Thr 
    1250                 


<210>  148
<211>  3804
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  148
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cttcaccact       60

aggactcagc tgcctcccgc ttacaccaac tcattcacta ggggtgtgta ctacccagac      120

aaggtcttcc gctcctcagt gctgcactca actcaggacc tgttcctgcc tttcttctcc      180

aacgtcacct ggttccacgc tatccacgtg tccggcacca acggcactaa gaggttcgcc      240

aaccccgtgc tgccattcaa cgacggagtc tacttcgctt caaccgagaa gtccaacatc      300

atcagaggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcta ccaacgtggt catcaaggtg tgcgaattcc agttctgtaa cgaccccttc      420

ctgggtgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagattt acagcaagca cacccccatc aacctggtcc gtggcctgcc acagggattc      660

tctgctctgg agcccctggt ggacctgcca atcggcatca acatcaccag gttccagact      720

ctgcacagat cctacctgac tcccggagac tcttcatccg gttggaccgc tggcgctgcc      780

gcttactacg tgggttacct gcagccacgc accttcctgc tgaagtacaa cgaaaacggc      840

accatcactg acgccgtcga ctgcgctctg gacccactgt cagaaaccaa gtgcactctg      900

aagtccttca ccgtggagaa gggaatctac cagacttcaa acttccgcgt gcagcctacc      960

gaatccatcg tccgtttccc aaacatcact aacctgtgcc ctttcggaga ggtcttcaac     1020

gccacccgct tcgcttccgt gtacgcctgg aaccgcaagc gtatctcaaa ctgcgtcgct     1080

gactactccg tgctgtacaa ctcagcctcc ttcagcacct tcaagtgcta cggagtgtca     1140

cccactaagc tgaacgacct gtgcttcacc aacgtctacg ctgactcctt cgtgatccgc     1200

ggtgacgagg tccgtcagat cgctccaggc cagactggaa acatcgccga ctacaactac     1260

aagctgcctg acgacttcac cggttgcgtc atcgcctgga acagcaacaa cctggactct     1320

aaagtgggtg gcaactacaa ctacctgtac aggctgttca gaaagtcaaa cctgaagccc     1380

ttcgagcgcg acatcagcac tgaaatctac caggctggtt ctaccccatg caacggtgtc     1440

aagggcttca actgctactt ccctctgcag tcctacggct tccagcccac ttacggtgtg     1500

ggctaccagc cataccgtgt ggtcgtgctg agcttcgagc tgctgcacgc ccctgctact     1560

gtgtgcggac ccaagaagtc taccaacctg gtcaagaaca agtgcgtgaa cttcaacttc     1620

aacggtctga ccggaactgg tgtcctgacc gagagcaaca agaagttcct gcctttccag     1680

cagttcggtc gcgacatcgc tgacaccact gacgccgtgc gtgaccctca gaccctggaa     1740

atcctggaca tcactccctg ctcattcgga ggtgtctccg tgatcactcc cggaaccaac     1800

acttctaacc aggtcgctgt gctgtaccag ggtgtgaact gcaccgaagt cccagtggcc     1860

atccacgctg accagctgac ccctacttgg agggtgtact ccaccggcag caacgtcttc     1920

cagactagag ctggatgcct gatcggtgcc gagcacgtca acaacagcta cgaatgcgac     1980

atccctatcg gcgctggaat ctgcgcctct taccagaccc agactaacag cccaggtggt     2040

tctgtggcct ctcagtcaat catcgcctac accatgtcac tgggtgtcga aaactccgtg     2100

gcttactcta acaactcaat cgccatccct accaacttca ctatcagcgt gaccactgag     2160

atcctgcccg tcagcatgac caagacttct gtggactgca ctatgtacat ctgcggcgac     2220

agcaccgaat gctctaacct gctgctgcag tacggttctt tctgcaccca gctgaaccgc     2280

gctctgactg gcatcgccgt ggagcaggac aagaacactc aggaagtctt cgctcaggtg     2340

aagcaaatct acaagacccc acctatcaag gacttcggcg gattcaactt ctcccagatc     2400

ctgccagacc cttccaagcc cagcaagagg tctttcatcg aggacctgct gttcaacaag     2460

gtcactctgg ccgacgctgg tttcatcaag cagtacggcg actgcctggg agacatcgct     2520

gctcgcgacc tgatctgcgc ccagaagttc aacggcctga ccgtgctgcc cccactgctg     2580

actgacgaaa tgatcgctca gtacactagc gccctgctgg ctggcaccat cacttcaggt     2640

tggactttcg gtgctggtgc tgctctgcag atcccattcg ctatgcagat ggcctaccgt     2700

ttcaacggaa tcggtgtcac ccagaacgtg ctgtacgaga accagaagct gatcgctaac     2760

cagttcaact cagccatcgg caagatccag gacagcctga gctctactgc ctctgctctg     2820

ggaaagctgc aggacgtcgt gaaccagaac gcccaggctc tgaacaccct ggtcaagcag     2880

ctgtcatcca acttcggcgc catcagctct gtgctgaacg acatcctgtc caggctggac     2940

cctcccgagg ctgaagtcca gatcgacagg ctgatcactg gaagactgca gtcactgcag     3000

acctacgtga ctcagcagct gatccgcgcc gctgaaatcc gtgcctccgc taacctggcc     3060

gctaccaaga tgagcgagtg cgtcctgggc cagtctaaga gagtggactt ctgcggcaag     3120

ggataccacc tgatgtcatt cccacagtcc gcccctcacg gagtcgtgtt cctgcacgtc     3180

acctacgtgc ccgctcagga gaagaacttc accactgccc cagctatctg ccacgacggc     3240

aaggctcact tccctcgcga aggagtcttc gtgagcaacg gcacccactg gttcgtcact     3300

cagcgtaact tctacgagcc ccagatcatc accactgaca acactttcgt gtctggcaac     3360

tgcgacgtgg tcatcggtat cgtcaacaac accgtgtacg accctctgca gcccgagctg     3420

gactcattca aggaagagct ggacaagtac ttcaagaacc acacctcccc tgacgtcgac     3480

ctgggtgaca tctcaggcat caacgcctcc gtcgtgaaca tccagaagga gatcgaccgc     3540

ctgaacgaag tggctaagaa cctgaacgaa agcctgatcg acctgcagga gctgggcaag     3600

tacgaacagt acatcaagtg gccatggtac atctggctgg gattcatcgc tggcctcatc     3660

gctatcgtga tggtgaccat catgctgtgc tgcatgactt catgctgctc ctgcctgaag     3720

ggttgctgca gctgcggctc ttgctgcaag ttcgacgagg acgactctga acctgtcctg     3780

aagggagtga agctgcacta cacc                                            3804


<210>  149
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  149

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Ser Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Tyr Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Ile Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Phe Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  150
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  150
atgttcgtct tcctggtgct gctgcctctg gtgtccagcc agtgcgtgaa cttcaccaac       60

cgcactcagc tgcctagcgc ttacaccaac tctttcacta ggggtgtgta ctaccccgac      120

aaggtcttcc gctcctcagt gctgcactct actcaggacc tgttcctgcc cttcttctct      180

aacgtcacct ggttccacgc catccacgtg agcggcacca acggcactaa gaggttcgac      240

aacccagtgc tgcctttcaa cgacggtgtc tacttcgctt caaccgagaa gtccaacatc      300

atcagaggat ggatcttcgg caccactctg gacagcaaga ctcagtctct gctgatcgtc      360

aacaacgcca ccaacgtggt catcaaggtg tgcgaattcc agttctgcaa ctacccattc      420

ctgggcgtct actaccacaa gaacaacaag tcatggatgg agtccgaatt ccgcgtctac      480

tccagcgcta acaactgcac tttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggaaagcagg gtaacttcaa gaacctgtca gagttcgtgt tcaagaacat cgacggatac      600

ttcaagattt actccaagca caccccaatc aacctggtcc gtgacctgcc tcagggtttc      660

tcagctctgg agccactggt ggacctgcct atcggcatca acatcaccag gttccagact      720

ctgctggctc tgcacagatc ctacctgact ccaggtgact catcttctgg atggactgct      780

ggagctgctg cttactacgt gggttacctg cagcctcgca ccttcctgct gaagtacaac      840

gaaaacggca ccatcactga cgccgtcgac tgcgctctgg accctctgag cgaaaccaag      900

tgcactctga agtctttcac cgtggagaag ggtatctacc agactagcaa cttccgcgtg      960

cagcccaccg aatctatcgt ccgtttccct aacatcacta acctgtgccc cttcggcgag     1020

gtcttcaacg ccacccgttt cgcttccgtg tacgcctgga accgcaagcg tatcagcaac     1080

tgcgtcgctg actactctgt gctgtacaac tcagcctcct tcagcacttt caagtgctac     1140

ggtgtgagcc ctactaagct gaacgacctg tgcttcacca acgtctacgc cgactctttc     1200

gtgatccgcg gcgacgaggt ccgtcagatc gctccaggcc agaccggaac tatcgccgac     1260

tacaactaca agctgcctga cgacttcacc ggctgcgtca tcgcttggaa ctcaaacaac     1320

ctggactcca aagtgggtgg caactacaac tacctgtaca ggctgttcag aaagtcaaac     1380

ctgaagccct tcgagaggga catctcaact gaaatctacc aggccggctc caccccatgc     1440

aacggtgtca agggcttcaa ctgctacttc cccctgcagt cctacggatt ccagccaact     1500

tacggtgtgg gctaccagcc ttacagagtg gtcgtgctgt cattcgaact cctccacgct     1560

cctgctactg tgtgcggacc aaagaagtcc accaacctgg tcaagaacaa gtgcgtgaac     1620

ttcaacttca acggtctgac cggaactggt gtcctgaccg agtcaaacaa gaagttcctg     1680

cccttccagc agttcggtcg cgacatcgct gacactaccg acgccgtgcg tgacccccag     1740

accctggaaa tcctggacat cactccatgc agcttcggag gtgtctctgt gatcactcca     1800

ggaaccaaca cttccaacca ggtcgctgtg ctgtaccagg gtgtcaactg caccgaagtc     1860

cctgtggcca tccacgctga ccagctgacc cccacttgga gggtgtactc caccggcagc     1920

aacgtcttcc agactagagc tggttgcctg atcggcgccg agtacgtgaa caactcatac     1980

gaatgcgaca tccctatcgg cgctggaatc tgcgcctcct accagaccca gactaactca     2040

ccccagcagg ctcagtccgt cgcctctcag tcaatcatcg cttacaccat gagcctgggc     2100

gctgaaaact ctgtggccta ctctaacaac tcaatcgcca tccccaccaa cttcactatc     2160

agcgtgacca ctgagatcct gccagtctca atgaccaaga cttccgtgga ctgcactatg     2220

tacatctgcg gagacagcac cgaatgctct aacctgctgc tgcagtacgg ctctttctgc     2280

acccagctga acagggctct gactggaatc gccgtggagc aggacaagaa cactcaggaa     2340

gtcttcgctc aggtgaagca aatctacaag acccctccca tcaaggactt cggcggattc     2400

aacttctccc agatcctgcc tgacccctcc aagcccagca agcgctcttt catcgaggac     2460

ctgctgttca acaaggtcac cctggccgac gctggcttca tcaagcagta cggagactgc     2520

ctgggtgaca tcgccgctcg tgacctgatc tgcgctcaga agttcaacgg actgaccgtg     2580

ctgccacctc tgctgactga cgaaatgatc gcccagtaca cttcagccct gctggctgga     2640

accatcactt ctggttggac cttcggtgct ggtgctgctc tgcagatccc attcgctatg     2700

cagatggcct accgcttcaa cggaatcggt gtcacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caacagcgcc atcggcaaga tccaggactc actgagctct     2820

actgcctccg ctctgggaaa gctgcaggac gtcgtgaacc agaacgccca ggctctgaac     2880

accctggtca agcagctgtc atccaacttc ggagctatca gctctgtgct gaacgacatc     2940

ctctcccgcc tggaccctcc tgaggctgaa gtccagatcg acaggctgat cactggtaga     3000

ctgcagagcc tgcagaccta cgtgactcag cagctgatcc gcgccgctga aatccgtgcc     3060

tctgctaacc tggctgctat caagatgtca gagtgcgtcc tgggacagtc caagagagtg     3120

gacttctgcg gcaagggata ccacctgatg tcattccctc agtccgctcc ccacggtgtc     3180

gtgttcctgc acgtcaccta cgtgccagcc caggagaaga acttcaccac tgcccctgct     3240

atctgccacg acggcaaggc tcacttcccc cgcgaaggtg tcttcgtgag caacggcacc     3300

cactggttcg tcactcagcg taacttctac gagccacaga tcatcaccac tgacaacact     3360

ttcgtgtctg gaaactgcga cgtggtcatc ggtatcgtca acaacaccgt gtacgacccc     3420

ctgcagccag agctggacag cttcaaggaa gagctggaca agtacttcaa gaaccacacc     3480

tctcccgacg tggacctggg cgacatcagc ggaatcaacg cttctttcgt caacatccag     3540

aaggagatcg accgtctgaa cgaagtggcc aagaacctga acgaatcact gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aagtggccat ggtacatctg gctgggtttc     3660

atcgctggcc tcatcgctat cgtgatggtg accatcatgc tgtgctgcat gacttcatgc     3720

tgctcctgcc tgaagggctg ctgcagctgc ggatcttgct gcaagttcga cgaggacgac     3780

tccgaacctg tcctgaaggg agtgaagctg cactacacct aa                        3822


<210>  151
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  151

Gln Cys Val Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Ser Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu Tyr Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Ile Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Phe Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  152
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  152

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ile Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Cys Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Pro Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  153
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  153

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Cys Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Pro Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  154
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal signal peptide

<400>  154

Met Phe Val Phe Phe Val Leu Leu Pro Leu Val Ser Ser 
1               5                   10              


<210>  155
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  155

Met Phe Val Phe Phe Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Gly Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  156
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  156

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Ile Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Gly 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  157
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  157

Met Phe Val Phe Phe Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Gly Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Asn Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  158
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified coronavirus S polypeptide

<400>  158

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Ile Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Gly 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Asn 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  159
<211>  1271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  159

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Gly Val Tyr Ser Ser 
145                 150                 155                 160 


Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp 
                165                 170                 175     


Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe 
            180                 185                 190         


Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
        195                 200                 205             


Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu 
    210                 215                 220                 


Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu 
225                 230                 235                 240 


Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp 
                245                 250                 255     


Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr 
            260                 265                 270         


Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp 
        275                 280                 285             


Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe 
    290                 295                 300                 


Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro 
305                 310                 315                 320 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
                325                 330                 335     


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
            340                 345                 350         


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
        355                 360                 365             


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
    370                 375                 380                 


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
385                 390                 395                 400 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
                405                 410                 415     


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
            420                 425                 430         


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
        435                 440                 445             


Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
    450                 455                 460                 


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys Asn Gly 
465                 470                 475                 480 


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
                485                 490                 495     


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
            500                 505                 510         


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
        515                 520                 525             


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu 
    530                 535                 540                 


Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe 
545                 550                 555                 560 


Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp 
                565                 570                 575     


Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly 
            580                 585                 590         


Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val 
        595                 600                 605             


Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala 
    610                 615                 620                 


Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val 
625                 630                 635                 640 


Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn 
                645                 650                 655     


Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr 
            660                 665                 670         


Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
785                 790                 795                 800 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
                805                 810                 815     


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
            820                 825                 830         


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
        835                 840                 845             


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
    850                 855                 860                 


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
865                 870                 875                 880 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
                885                 890                 895     


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
            900                 905                 910         


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
        915                 920                 925             


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
    930                 935                 940                 


Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
945                 950                 955                 960 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
                965                 970                 975     


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
            980                 985                 990         


Arg Leu Ile Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
        995                 1000                 1005             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1010                 1015                 1020             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1025                 1030                 1035             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1040                 1045                 1050             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1055                 1060                 1065             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1070                 1075                 1080             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1085                 1090                 1095             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1100                 1105                 1110             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1115                 1120                 1125             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1130                 1135                 1140             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1145                 1150                 1155             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1160                 1165                 1170             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1175                 1180                 1185             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1190                 1195                 1200             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1205                 1210                 1215             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1220                 1225                 1230             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1235                 1240                 1245             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1250                 1255                 1260             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1265                 1270     


<210>  160
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  160

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Lys Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  His Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  161
<211>  1271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  161

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Gly Val Tyr Ser Ser 
145                 150                 155                 160 


Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp 
                165                 170                 175     


Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe 
            180                 185                 190         


Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
        195                 200                 205             


Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu 
    210                 215                 220                 


Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu 
225                 230                 235                 240 


Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Ile 
                245                 250                 255     


Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr 
            260                 265                 270         


Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp 
        275                 280                 285             


Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe 
    290                 295                 300                 


Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro 
305                 310                 315                 320 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
                325                 330                 335     


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
            340                 345                 350         


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
        355                 360                 365             


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
    370                 375                 380                 


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
385                 390                 395                 400 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile 
                405                 410                 415     


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
            420                 425                 430         


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
        435                 440                 445             


Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
    450                 455                 460                 


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys Asn Gly 
465                 470                 475                 480 


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
                485                 490                 495     


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
            500                 505                 510         


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
        515                 520                 525             


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu 
    530                 535                 540                 


Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe 
545                 550                 555                 560 


Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp 
                565                 570                 575     


Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly 
            580                 585                 590         


Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val 
        595                 600                 605             


Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala 
    610                 615                 620                 


Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val 
625                 630                 635                 640 


Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn 
                645                 650                 655     


Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr 
            660                 665                 670         


Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
785                 790                 795                 800 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
                805                 810                 815     


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
            820                 825                 830         


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
        835                 840                 845             


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
    850                 855                 860                 


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
865                 870                 875                 880 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
                885                 890                 895     


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
            900                 905                 910         


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
        915                 920                 925             


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
    930                 935                 940                 


Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
945                 950                 955                 960 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
                965                 970                 975     


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
            980                 985                 990         


Arg Leu Ile Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
        995                 1000                 1005             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1010                 1015                 1020             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1025                 1030                 1035             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1040                 1045                 1050             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1055                 1060                 1065             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1070                 1075                 1080             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1085                 1090                 1095             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1100                 1105                 1110             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1115                 1120                 1125             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1130                 1135                 1140             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1145                 1150                 1155             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1160                 1165                 1170             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1175                 1180                 1185             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1190                 1195                 1200             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1205                 1210                 1215             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1220                 1225                 1230             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1235                 1240                 1245             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1250                 1255                 1260             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1265                 1270     


<210>  162
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  162

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Ser 
    130                 135                 140                 


Asn His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser His Gln Gln Ala Gln Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  163
<211>  1274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  163

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Thr 
    130                 135                 140                 


Ser Asn His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val 
145                 150                 155                 160 


Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe 
                165                 170                 175     


Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu 
            180                 185                 190         


Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His 
        195                 200                 205             


Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu 
    210                 215                 220                 


Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln 
225                 230                 235                 240 


Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
                245                 250                 255     


Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln 
            260                 265                 270         


Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp 
        275                 280                 285             


Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
    290                 295                 300                 


Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
305                 310                 315                 320 


Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
                325                 330                 335     


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Ala Ser Val Tyr 
            340                 345                 350         


Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
        355                 360                 365             


Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
    370                 375                 380                 


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
385                 390                 395                 400 


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
                405                 410                 415     


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
            420                 425                 430         


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
        435                 440                 445             


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
    450                 455                 460                 


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
465                 470                 475                 480 


Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
                485                 490                 495     


Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
            500                 505                 510         


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
        515                 520                 525             


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
    530                 535                 540                 


Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe 
545                 550                 555                 560 


Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala 
                565                 570                 575     


Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser 
            580                 585                 590         


Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln 
        595                 600                 605             


Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala 
    610                 615                 620                 


Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly 
625                 630                 635                 640 


Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His 
                645                 650                 655     


Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys 
            660                 665                 670         


Ala Ser Tyr Gln Thr Gln Thr Asn Ser His Gln Gln Ala Gln Ser Val 
        675                 680                 685             


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn 
    690                 695                 700                 


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
705                 710                 715                 720 


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
                725                 730                 735     


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
            740                 745                 750         


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
        755                 760                 765             


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
    770                 775                 780                 


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
785                 790                 795                 800 


Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg 
                805                 810                 815     


Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
            820                 825                 830         


Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg 
        835                 840                 845             


Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro 
    850                 855                 860                 


Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala 
865                 870                 875                 880 


Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln 
                885                 890                 895     


Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val 
            900                 905                 910         


Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe 
        915                 920                 925             


Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser 
    930                 935                 940                 


Ala Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu 
945                 950                 955                 960 


Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
                965                 970                 975     


Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val 
            980                 985                 990         


Gln Ile Asp Arg Leu Ile Thr Gly  Arg Leu Gln Ser Leu  Gln Thr Tyr 
        995                 1000                 1005             


Val Thr  Gln Gln Leu Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala 
    1010                 1015                 1020             


Asn Leu  Ala Ala Thr Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser 
    1025                 1030                 1035             


Lys Arg  Val Asp Phe Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe 
    1040                 1045                 1050             


Pro Gln  Ser Ala Pro His Gly  Val Val Phe Leu His  Val Thr Tyr 
    1055                 1060                 1065             


Val Pro  Ala Gln Glu Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys 
    1070                 1075                 1080             


His Asp  Gly Lys Ala His Phe  Pro Arg Glu Gly Val  Phe Val Ser 
    1085                 1090                 1095             


Asn Gly  Thr His Trp Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro 
    1100                 1105                 1110             


Gln Ile  Ile Thr Thr Asp Asn  Thr Phe Val Ser Gly  Asn Cys Asp 
    1115                 1120                 1125             


Val Val  Ile Gly Ile Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln 
    1130                 1135                 1140             


Pro Glu  Leu Asp Ser Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys 
    1145                 1150                 1155             


Asn His  Thr Ser Pro Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile 
    1160                 1165                 1170             


Asn Ala  Ser Val Val Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn 
    1175                 1180                 1185             


Glu Val  Ala Lys Asn Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu 
    1190                 1195                 1200             


Leu Gly  Lys Tyr Glu Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp 
    1205                 1210                 1215             


Leu Gly  Phe Ile Ala Gly Leu  Ile Ala Ile Val Met  Val Thr Ile 
    1220                 1225                 1230             


Met Leu  Cys Cys Met Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys 
    1235                 1240                 1245             


Cys Ser  Cys Gly Ser Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu 
    1250                 1255                 1260             


Pro Val  Leu Lys Gly Val Lys  Leu His Tyr Thr 
    1265                 1270                 


<210>  164
<211>  1258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  164

Gln Cys Val Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Asp Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Gly Val 
    130                 135                 140                 


Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe 
145                 150                 155                 160 


Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu 
                165                 170                 175     


Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His 
            180                 185                 190         


Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu 
        195                 200                 205             


Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln 
    210                 215                 220                 


Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
225                 230                 235                 240 


Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln 
                245                 250                 255     


Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp 
            260                 265                 270         


Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
        275                 280                 285             


Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
    290                 295                 300                 


Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
305                 310                 315                 320 


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
                325                 330                 335     


Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
            340                 345                 350         


Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
        355                 360                 365             


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
    370                 375                 380                 


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
385                 390                 395                 400 


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
                405                 410                 415     


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
            420                 425                 430         


Asn Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
        435                 440                 445             


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro 
    450                 455                 460                 


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
465                 470                 475                 480 


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
                485                 490                 495     


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
            500                 505                 510         


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
        515                 520                 525             


Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe 
    530                 535                 540                 


Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala 
545                 550                 555                 560 


Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser 
                565                 570                 575     


Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln 
            580                 585                 590         


Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala 
        595                 600                 605             


Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly 
    610                 615                 620                 


Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His 
625                 630                 635                 640 


Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys 
                645                 650                 655     


Ala Ser Tyr Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln Ser Val 
            660                 665                 670         


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn 
        675                 680                 685             


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
    690                 695                 700                 


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
705                 710                 715                 720 


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
                725                 730                 735     


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
            740                 745                 750         


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
        755                 760                 765             


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
    770                 775                 780                 


Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg 
785                 790                 795                 800 


Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
                805                 810                 815     


Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg 
            820                 825                 830         


Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro 
        835                 840                 845             


Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala 
    850                 855                 860                 


Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln 
865                 870                 875                 880 


Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val 
                885                 890                 895     


Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe 
            900                 905                 910         


Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser 
        915                 920                 925             


Ala Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu 
    930                 935                 940                 


Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
945                 950                 955                 960 


Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val 
                965                 970                 975     


Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr 
            980                 985                 990         


Val Thr Gln Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
        995                 1000                 1005             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1010                 1015                 1020             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1025                 1030                 1035             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1040                 1045                 1050             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1055                 1060                 1065             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1070                 1075                 1080             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1085                 1090                 1095             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1100                 1105                 1110             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1115                 1120                 1125             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1130                 1135                 1140             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1145                 1150                 1155             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1160                 1165                 1170             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1175                 1180                 1185             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1190                 1195                 1200             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1205                 1210                 1215             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1220                 1225                 1230             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1235                 1240                 1245             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255             


<210>  165
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  165

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Ile Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Asp Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Lys Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala His Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  166
<211>  1258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  166

Gln Cys Val Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Asp Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Gly Val 
    130                 135                 140                 


Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe 
145                 150                 155                 160 


Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu 
                165                 170                 175     


Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His 
            180                 185                 190         


Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu 
        195                 200                 205             


Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln 
    210                 215                 220                 


Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
225                 230                 235                 240 


Ser Gly Ile Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln 
                245                 250                 255     


Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp 
            260                 265                 270         


Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
        275                 280                 285             


Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
    290                 295                 300                 


Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
305                 310                 315                 320 


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
                325                 330                 335     


Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
            340                 345                 350         


Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
        355                 360                 365             


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
    370                 375                 380                 


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
385                 390                 395                 400 


Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
                405                 410                 415     


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
            420                 425                 430         


Asn Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
        435                 440                 445             


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro 
    450                 455                 460                 


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
465                 470                 475                 480 


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
                485                 490                 495     


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
            500                 505                 510         


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
        515                 520                 525             


Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe 
    530                 535                 540                 


Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala 
545                 550                 555                 560 


Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser 
                565                 570                 575     


Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln 
            580                 585                 590         


Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala 
        595                 600                 605             


Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly 
    610                 615                 620                 


Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His 
625                 630                 635                 640 


Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys 
                645                 650                 655     


Ala Ser Tyr Gln Thr Gln Thr Asn Ser Arg Gln Gln Ala Gln Ser Val 
            660                 665                 670         


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn 
        675                 680                 685             


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
    690                 695                 700                 


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
705                 710                 715                 720 


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
                725                 730                 735     


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
            740                 745                 750         


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
        755                 760                 765             


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
    770                 775                 780                 


Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg 
785                 790                 795                 800 


Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
                805                 810                 815     


Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg 
            820                 825                 830         


Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro 
        835                 840                 845             


Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala 
    850                 855                 860                 


Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln 
865                 870                 875                 880 


Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val 
                885                 890                 895     


Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe 
            900                 905                 910         


Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser 
        915                 920                 925             


Ala Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu 
    930                 935                 940                 


Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
945                 950                 955                 960 


Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val 
                965                 970                 975     


Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr 
            980                 985                 990         


Val Thr Gln Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
        995                 1000                 1005             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1010                 1015                 1020             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1025                 1030                 1035             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1040                 1045                 1050             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1055                 1060                 1065             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1070                 1075                 1080             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1085                 1090                 1095             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1100                 1105                 1110             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1115                 1120                 1125             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1130                 1135                 1140             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1145                 1150                 1155             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1160                 1165                 1170             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1175                 1180                 1185             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1190                 1195                 1200             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1205                 1210                 1215             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1220                 1225                 1230             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1235                 1240                 1245             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1250                 1255             


<210>  167
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  167

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Ile Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Ser Asn His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe 
    130                 135                 140                 


Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln 
145                 150                 155                 160 


Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu 
                165                 170                 175     


Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser 
            180                 185                 190         


Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser 
        195                 200                 205             


Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg 
    210                 215                 220                 


Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp 
225                 230                 235                 240 


Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr 
                245                 250                 255     


Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
            260                 265                 270         


Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys 
        275                 280                 285             


Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn 
    290                 295                 300                 


Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
305                 310                 315                 320 


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Ala Ser 
                325                 330                 335     


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            340                 345                 350         


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        355                 360                 365             


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    370                 375                 380                 


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
385                 390                 395                 400 


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                405                 410                 415     


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            420                 425                 430         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        435                 440                 445             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    450                 455                 460                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
465                 470                 475                 480 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                485                 490                 495     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            500                 505                 510         


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
        515                 520                 525             


Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys 
    530                 535                 540                 


Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro 
                565                 570                 575     


Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser 
            580                 585                 590         


Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro 
        595                 600                 605             


Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser 
    610                 615                 620                 


Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala 
625                 630                 635                 640 


Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly 
                645                 650                 655     


Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser His Gln Gln Ala Gln 
            660                 665                 670         


Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala 
        675                 680                 685             


Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn 
    690                 695                 700                 


Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys 
705                 710                 715                 720 


Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys 
                725                 730                 735     


Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
            740                 745                 750         


Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val 
        755                 760                 765             


Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe 
    770                 775                 780                 


Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser 
785                 790                 795                 800 


Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala 
                805                 810                 815     


Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala 
            820                 825                 830         


Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu 
        835                 840                 845             


Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu 
    850                 855                 860                 


Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala 
865                 870                 875                 880 


Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile 
                885                 890                 895     


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            900                 905                 910         


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        915                 920                 925             


Ala Ser Ala Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln 
    930                 935                 940                 


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
945                 950                 955                 960 


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala 
                965                 970                 975     


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            980                 985                 990         


Thr Tyr Val Thr Gln Gln Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser 
        995                 1000                 1005             


Ala Asn  Leu Ala Ala Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln 
    1010                 1015                 1020             


Ser Lys  Arg Val Asp Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser 
    1025                 1030                 1035             


Phe Pro  Gln Ser Ala Pro His  Gly Val Val Phe Leu  His Val Thr 
    1040                 1045                 1050             


Tyr Val  Pro Ala Gln Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile 
    1055                 1060                 1065             


Cys His  Asp Gly Lys Ala His  Phe Pro Arg Glu Gly  Val Phe Val 
    1070                 1075                 1080             


Ser Asn  Gly Thr His Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu 
    1085                 1090                 1095             


Pro Gln  Ile Ile Thr Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys 
    1100                 1105                 1110             


Asp Val  Val Ile Gly Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu 
    1115                 1120                 1125             


Gln Pro  Glu Leu Asp Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe 
    1130                 1135                 1140             


Lys Asn  His Thr Ser Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly 
    1145                 1150                 1155             


Ile Asn  Ala Ser Val Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu 
    1160                 1165                 1170             


Asn Glu  Val Ala Lys Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln 
    1175                 1180                 1185             


Glu Leu  Gly Lys Tyr Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile 
    1190                 1195                 1200             


Trp Leu  Gly Phe Ile Ala Gly  Leu Ile Ala Ile Val  Met Val Thr 
    1205                 1210                 1215             


Ile Met  Leu Cys Cys Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly 
    1220                 1225                 1230             


Cys Cys  Ser Cys Gly Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser 
    1235                 1240                 1245             


Glu Pro  Val Leu Lys Gly Val  Lys Leu His Tyr Thr  
    1250                 1255                 1260 


<210>  168
<211>  1261
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SARS-CoV-2 S Polypeptide

<400>  168

Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr 
1               5                   10                  15      


Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
            20                  25                  30          


Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn 
        35                  40                  45              


Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys 
    50                  55                  60                  


Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala 
65                  70                  75                  80  


Ser Ile Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr 
                85                  90                  95      


Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn 
            100                 105                 110         


Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu 
        115                 120                 125             


Gly Val Thr Ser Asn His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu 
    130                 135                 140                 


Phe Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser 
145                 150                 155                 160 


Gln Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn 
                165                 170                 175     


Leu Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr 
            180                 185                 190         


Ser Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe 
        195                 200                 205             


Ser Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr 
    210                 215                 220                 


Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly 
225                 230                 235                 240 


Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly 
                245                 250                 255     


Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr 
            260                 265                 270         


Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys 
        275                 280                 285             


Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser 
    290                 295                 300                 


Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile 
305                 310                 315                 320 


Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Ala 
                325                 330                 335     


Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            340                 345                 350         


Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        355                 360                 365             


Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    370                 375                 380                 


Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
385                 390                 395                 400 


Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                405                 410                 415     


Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            420                 425                 430         


Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        435                 440                 445             


Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    450                 455                 460                 


Ser Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu 
465                 470                 475                 480 


Gln Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr 
                485                 490                 495     


Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            500                 505                 510         


Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn 
        515                 520                 525             


Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn 
    530                 535                 540                 


Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr 
545                 550                 555                 560 


Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr 
                565                 570                 575     


Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr 
            580                 585                 590         


Ser Asn Gln Val Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val 
        595                 600                 605             


Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr 
    610                 615                 620                 


Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly 
625                 630                 635                 640 


Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala 
                645                 650                 655     


Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser His Gln Gln Ala 
            660                 665                 670         


Gln Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly 
        675                 680                 685             


Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr 
    690                 695                 700                 


Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr 
705                 710                 715                 720 


Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu 
                725                 730                 735     


Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn 
            740                 745                 750         


Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu 
        755                 760                 765             


Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp 
    770                 775                 780                 


Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro 
785                 790                 795                 800 


Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu 
                805                 810                 815     


Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile 
            820                 825                 830         


Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val 
        835                 840                 845             


Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala 
    850                 855                 860                 


Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala 
865                 870                 875                 880 


Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly 
                885                 890                 895     


Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala 
            900                 905                 910         


Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser 
        915                 920                 925             


Thr Ala Ser Ala Leu Gly Lys Leu Gln Asn Val Val Asn Gln Asn Ala 
    930                 935                 940                 


Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala 
945                 950                 955                 960 


Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu 
                965                 970                 975     


Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu 
            980                 985                 990         


Gln Thr Tyr Val Thr Gln Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala 
        995                 1000                 1005             


Ser Ala  Asn Leu Ala Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly 
    1010                 1015                 1020             


Gln Ser  Lys Arg Val Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met 
    1025                 1030                 1035             


Ser Phe  Pro Gln Ser Ala Pro  His Gly Val Val Phe  Leu His Val 
    1040                 1045                 1050             


Thr Tyr  Val Pro Ala Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala 
    1055                 1060                 1065             


Ile Cys  His Asp Gly Lys Ala  His Phe Pro Arg Glu  Gly Val Phe 
    1070                 1075                 1080             


Val Ser  Asn Gly Thr His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr 
    1085                 1090                 1095             


Glu Pro  Gln Ile Ile Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn 
    1100                 1105                 1110             


Cys Asp  Val Val Ile Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro 
    1115                 1120                 1125             


Leu Gln  Pro Glu Leu Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr 
    1130                 1135                 1140             


Phe Lys  Asn His Thr Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser 
    1145                 1150                 1155             


Gly Ile  Asn Ala Ser Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg 
    1160                 1165                 1170             


Leu Asn  Glu Val Ala Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu 
    1175                 1180                 1185             


Gln Glu  Leu Gly Lys Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr 
    1190                 1195                 1200             


Ile Trp  Leu Gly Phe Ile Ala  Gly Leu Ile Ala Ile  Val Met Val 
    1205                 1210                 1215             


Thr Ile  Met Leu Cys Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys 
    1220                 1225                 1230             


Gly Cys  Cys Ser Cys Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp 
    1235                 1240                 1245             


Ser Glu  Pro Val Leu Lys Gly  Val Lys Leu His Tyr  Thr 
    1250                 1255                 1260     


