                         SEQUENCE LISTING

<110>  Syngenta
       Bortiri, Esteban
       Kelliher, Tim
 
<120>  INDUCIBLE MOSAICISM

<130>  81776-INDYMOS

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  13484
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector 24902


<220>
<221>  promoter
<222>  (521)..(1045)
<223>  35S promoter

<220>
<221>  gene
<222>  (1071)..(2384)
<223>  cGal4VP16GR-n1 GAL4-binding domain-VP16 activation domain-GR (for
       glucocorticoid receptor) fusion (GVG)

<220>
<221>  terminator
<222>  (2396)..(3038)
<223>  PsE9 terminator

<400>  1
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt       60

taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc      120

tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct      180

tccctaatta gctaacccgg gggcgcgccg ggaccgagat tagccttttc aatttcagaa      240

agaatgctaa cccacagatg gttagagagg cttacgcagc aggtctcatc aagacgatct      300

acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca      360

aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa      420

gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga      480

ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa      540

tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac      600

gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact      660

ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa      720

gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga      780

agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca      840

tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca      900

tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct      960

ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat     1020

aaggaagttc atttcatttg gagagaacac gggggactct agtttaaaca atggcagatc     1080

caatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca     1140

agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct     1200

actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa     1260

ggctagaaag actggaacag ctatttctac tgatttttcc taggtcgagc gcccccccga     1320

ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg     1380

ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg     1440

gatttacccc ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg     1500

agcagatgtt taccgatgcc cttggaattg acgagtacgg tggggatcca attcagcaag     1560

ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata gttcctgctg     1620

cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa cccgaggtgt     1680

tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg accacactca     1740

acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg ataccaggct     1800

tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg atgtttctca     1860

tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg ctctgctttg     1920

ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat gaccaatgta     1980

aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat gaagagtatc     2040

tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg aagagccaag     2100

agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc atcgtcaaaa     2160

gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaag cttctggact     2220

ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt ttggataaga     2280

ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag ataccaaaat     2340

attcaaatgg aaatatcaaa aagcttctgt ttcatcaaaa atgacttgag ctcttagctt     2400

tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgcgc     2460

acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt ttttcttgta     2520

ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga     2580

aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt     2640

aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg     2700

caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat     2760

atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca acaaatatat     2820

tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct tgtgttttag     2880

acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc taatcattgc     2940

tttataatta tagttatact catggatttg tagttgagta tgaaaatatt ttttaatgca     3000

ttttatgact tgccaattga ttgacaacat gcatcaataa cctgcaggtc gaagcttgca     3060

tgccggtcga ctctagagga tccgggtgac agccctccga cgggtgacag ccctccgacg     3120

ggtgacagcc ctccgaattc tagaggatcc gggtgacagc cctccgacgg gtgacagccc     3180

tccgacgggt gacagccctc cgaattcgag ctcggtaccc ggggatctgt cgacctcgat     3240

cgagatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac     3300

gctgaagcta gtcgactcta gcctgcgatc gctaaaaaat ggccctgtcc aacaagttca     3360

tcggcgacga catgaagatg acctaccaca tggacggctg cgtgaacggc cactacttca     3420

ccgtgaaggg cgagggcagc ggcaagccct acgagggcac ccagacctcc accttcaagg     3480

tgaccatggc caacggcggc cccctggcct tctccttcga catcctgtcc accgtgttca     3540

tgtacggcaa ccgctgcttc accgcctacc ccaccagcat gcccgactac ttcaagcagg     3600

ccttccccga cggcatgtcc tacgagagaa ccttcaccta cgaggacggc ggcgtggcca     3660

ccgccagctg ggagatcagc ctgaagggca actgcttcga gcacaagtcc accttccacg     3720

gcgtgaactt ccccgccgac ggccccgtga tggccaagaa gaccaccggc tgggacccct     3780

ccttcgagaa gatgaccgtg tgcgacggca tcttgaaggg cgacgtgacc gccttcctga     3840

tgctgcaggg cggcggcaac tacagatgcc agttccacac ctcctacaag accaagaagc     3900

ccgtgaccat gccccccaac cacgtggtgg agcaccgcat cgccagaacc gacctggaca     3960

agggcggcaa cagcgtgcag ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc     4020

ccttctgaag taacgcgttt atgatcgttc aaacatttgg caataaagtt tcttaagatt     4080

gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca     4140

tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt     4200

cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa     4260

attatcgcgc gcggtgtcat ctatgttact agatcggacc gctaattagc taagagacac     4320

ttgtgtgatt gagagaaaca ctaatcttgt gaggactgaa gtttggtgat tatttcttgt     4380

gatctgtcga caaaaatatc aaatggggtt tcttttacaa attatttacc taaatgaatc     4440

tgttttgaaa atatttactc cattgggtct atttttttat tacaaagcgt ctccctgaag     4500

ggcgcgttcc ccgtgaaagt gacacgtggc aggacttggg acgtgccctg cgtacaggcg     4560

cgatagttag tgttgttaca gcaggcgcat cgggtcgtgt tggggaccaa ggtacgacag     4620

gtcgcgctgg gtgacccaga cacgacccaa ttgggtcgca ctttatttaa tattttttat     4680

attttgtata ttgtttttat ttaatatatt tttatattat tttatttaat ttttttatat     4740

tttatataat agtttctata ttaaataaat tcttagcatt atgtatgatt ttaaagtcat     4800

aaataatttt ttatattgtt tttatttact atatttttta tattttattt aatatttata     4860

tattaaataa atccttcata ttagaaaaaa taaagaaaat attaaataaa atataaaata     4920

taaaaaagta aaaaatatta aataaaataa tataaaaaat attataaaaa caatataaaa     4980

aatataaaaa tatttaataa aataataaaa aaaatattat tttaaataaa attatttatg     5040

actttaaact ctaaagttga attttaaaaa aatataattt ttttacgatt ttagtaaaaa     5100

aaaaatacaa gccgcacaat acaagtcgcc ttctcaaacc cttcctcacg acattctcgg     5160

accttatgac accgtcacca aaacaatgat ccacgcgata ttaggcgcgt gcaaatcact     5220

ctaatccgaa actagtagac atgggaagca cgagctatac gcgagcgttt caattgccgc     5280

cacgaaagca gagaaggcca gaaacggaac cacggtaaaa tggtaagggt attttcgtaa     5340

acagaagaaa agagttgtag ctataaataa accctctaac ccacggcgca ctatttctct     5400

tcactccttc gttcactctt cttctcttgc ggctagggtt ttagcgcagc ttcttctagg     5460

ttcgttctct tccgccgctc tatggatttt aaaccttcga atcatgttta ttccattgaa     5520

ttatgttgct tgcagtttat attttctgaa tctgtagttg ttgtcttcaa tttatcctat     5580

gctttataga tcaatctttt gtgtgtgtag tacgtaattt ttgttctttt tgcttttcgt     5640

tcaagttgtt gggaataatc ggggtatcat gttttgatat tgtttgtttt cttttttgac     5700

tgcttaataa tttttaagtt ggttttggtt ttggggtttt atgtgcttgt tatattcaaa     5760

tctttgtgat ccagatctta caaaagtttt gggtttaagg atgtttttgg ctgatgatga     5820

atagatctat aaactgttcc ttttaatcga ttcaagctta ggattttact aggcttttgc     5880

gaataaatac gtgacagtaa gctaattatg tccttttttt gtctcaatca tatctgtctg     5940

ggtgtgccat aatttgtgat atgtctatct ggtagaatct tgtgttttat gctttacgat     6000

ttggtatacc tgtttttgaa cttgttgtat gatgggtatt tagatcaccc tatctttttt     6060

atgcttctgg aagttttatg taaatgtcga atatcttaat gttgttgaac ttataatgtt     6120

gtgttgatgt atgtatgatg gttttgacaa cttttttcac tggttctgaa agttttatgt     6180

aaattgcaaa tatgttaatg ttgttgaact tatttttttt ccttcgatgt tgttttgatg     6240

tatgtatgat ggttttcacc gtagtttcta tggctaatat cttaatgttg ttgagcttat     6300

ttttttcctt atatgttgtg ttgatgtatt gtatgatggt tttgacaact tttttagttt     6360

ctttgcagat ttaaggaaga aaaaaaaaat ggctcttgtt gctagaccag tgctttctgc     6420

tagagttgct gcttctagac caagagttgc tgcaagaaag gctgttagag tgtctgctat     6480

gtctcatggt gcttcatcta gaccagctac tgctagaaag tcctctggac tttctggaac     6540

tgttaggatt ccaggcgata agtctatttc ccaccgttct ttcatgttcg gaggacttgc     6600

ttctggtgag actagaatta ctggacttct tgagggcgag gatgttatta acactggaaa     6660

ggctatgcag gctatgggag ctagaattag aaaagagggc gatacctgga ttattgatgg     6720

tgttggaaac ggtggacttc ttgctccaga agctccactt gatttcggaa acgctgctac     6780

tggatgcaga cttactatgg gacttgttgg agtgtacgat ttcgattcca ccttcattgg     6840

agatgcttct cttactaaga ggccaatggg aagagtgctt aacccactta gagagatggg     6900

agttcaggtt aagtctgagg atggtgatag gcttccagtt actcttagag gaccaaagac     6960

tccaacccca attacttaca gagtgccaat ggcttctgct caagttaagt ctgctgttct     7020

tcttgctgga cttaacactc caggtattac cactgtgatc gagccaatta tgaccagaga     7080

tcataccgag aagatgcttc aaggattcgg agctaacctt accgttgaaa ctgatgctga     7140

tggtgttagg accattagac ttgagggaag aggaaagctt accggacaag ttattgatgt     7200

tccaggtgat ccatcttcta ccgctttccc tcttgttgct gctcttttgg ttccaggatc     7260

tgatgtgacc attcttaacg tgctcatgaa cccatctagg accggactta ttcttaccct     7320

tcaagaaatg ggcgctgata ttgaggttat caacccaaga cttgctggtg gtgaagatgt     7380

tgctgatctt agggttaggt cctctactct taagggtgtt actgttccag aagatagggc     7440

tccatccatg attgatgagt acccaattct tgctgtggct gctgctttcg ctgaaggtgc     7500

tactgttatg aacggacttg aggaacttag ggtgaaagag tctgataggc tttctgctgt     7560

tgctaacgga cttaagctta acggtgtgga ttgtgatgag ggtgagactt ctcttgttgt     7620

tagaggtaga ccagatggaa agggacttgg aaacgcttct ggtgcagcag ttgcaactca     7680

tcttgatcat aggatcgcta tgtccttcct tgttatgggt cttgtttctg agaacccagt     7740

taccgttgat gatgctacta tgattgctac cagcttccca gagttcatgg atcttatggc     7800

tggacttgga gctaagattg agctttctga taccaaggct gcctgactaa ttagctaaga     7860

gcttgatccg tcgacctgca gatcgttcaa acatttggca ataaagtttc ttaagattga     7920

atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg     7980

taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc     8040

cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat     8100

tatcgcgcgc ggtgtcatct atgttactag atccctgcag gcccgggggc gcgccctaat     8160

tagctaacgg ccaggatcgc cgcgtgagcc tttagcaact agctagatta attaacgcaa     8220

tctgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc     8280

cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc     8340

atcagaatta attctcatgt ttgacagctt atcatcgact gcacggtgca ccaatgcttc     8400

tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata     8460

attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa     8520

cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc tcgtataatg     8580

tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatgagg gaagcgttga     8640

tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac     8700

cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca     8760

gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt     8820

tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg     8880

tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg     8940

aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca     9000

cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg     9060

taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc     9120

taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg     9180

tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg     9240

atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg     9300

aagctaggca ggcttatctt ggacaagaag atcgcttggc ctcgcgcgca gatcagttgg     9360

aagaatttgt tcactacgtg aaaggcgaga tcaccaaagt agtcggcaaa taaagctcta     9420

gtggatctcc gtacccaggg atctggctcg cggcggacgc acgacgccgg ggcgagacca     9480

taggcgatct cctaaatcaa tagtagctgt aacctcgaag cgtttcactt gtaacaacga     9540

ttgagaattt ttgtcataaa attgaaatac ttggttcgca tttttgtcat ccgcggtcag     9600

ccgcaattct gacgaactgc ccatttagct ggagatgatt gtacatcctt cacgtgaaaa     9660

tttctcaagc gctgtgaaca agggttcaga ttttagattg aaaggtgagc cgttgaaaca     9720

cgttcttctt gtcgatgacg acgtcgctat gcggcatctt attattgaat accttacgat     9780

ccacgccttc aaagtgaccg cggtagccga cagcacccag ttcacaagag tactctcttc     9840

cgcgacggtc gatgtcgtgg ttgttgatct agatttaggt cgtgaagatg ggctcgagat     9900

cgttcgtaat ctggcggcaa agtctgatat tccaatcata attatcagtg gcgaccgcct     9960

tgaggagacg gataaagttg ttgcactcga gctaggagca agtgatttta tcgctaagcc    10020

gttcagtatc agagagtttc tagcacgcat tcgggttgcc ttgcgcgtgc gccccaacgt    10080

tgtccgctcc aaagaccgac ggtctttttg ttttactgac tggacactta atctcaggca    10140

acgtcgcttg atgtccgaag ctggcggtga ggtgaaactt acggcaggtg agttcaatct    10200

tctcctcgcg tttttagaga aaccccgcga cgttctatcg cgcgagcaac ttctcattgc    10260

cagtcgagta cgcgacgagg aggtttatga caggagtata gatgttctca ttttgaggct    10320

gcgccgcaaa cttgaggcag atccgtcaag ccctcaactg ataaaaacag caagaggtgc    10380

cggttatttc tttgacgcgg acgtgcaggt ttcgcacggg gggacgatgg cagcctgagc    10440

caattcccag atccccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa    10500

atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag    10560

cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga    10620

atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc    10680

aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat    10740

agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc    10800

gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc    10860

atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc    10920

atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt    10980

gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta    11040

gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag    11100

aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta    11160

aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tggctgattg gatgtaccgc    11220

gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc    11280

gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa    11340

gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag    11400

ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag    11460

gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc    11520

gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg    11580

gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg    11640

tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca    11700

cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta    11760

aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca    11820

gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct    11880

tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat    11940

ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct    12000

cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa    12060

gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac    12120

ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac    12180

tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct    12240

gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa    12300

actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta    12360

atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg    12420

cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt    12480

tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagctctg    12540

cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct    12600

tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac    12660

tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga    12720

gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat    12780

aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac    12840

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct    12900

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg    12960

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg    13020

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt    13080

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg    13140

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac    13200

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga    13260

aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt    13320

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt    13380

tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga    13440

ttatcaaaaa ggatcttcac ctagatcctt ttgatccgga atta                     13484


<210>  2
<211>  15227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector 25657


<220>
<221>  promoter
<222>  (217)..(1376)
<223>  AtEF1aA1 promoter

<220>
<221>  gene
<222>  (1395)..(6008)
<223>  GR-LbCas12a  Rice codon-optimized cLbCas12a-02 without NLS, 
       adding a glucocorticoid receptor (GR) binding domain at the 
       N-terminus separated by a long linker.

<220>
<221>  terminator
<222>  (6017)..(6269)
<223>  tNOS

<220>
<221>  promoter
<222>  (6288)..(7901)
<223>  prGmSAMS

<220>
<221>  misc_feature
<222>  (7914)..(7950)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (7951)..(7994)
<223>  LbCas12a guide RNA targeting AtGL1

<220>
<221>  misc_feature
<222>  (7995)..(8062)
<223>  HDV ribozyme

<220>
<221>  terminator
<222>  (8063)..(8408)
<223>  tAtUbq3

<400>  2
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt       60

taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc      120

tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct      180

tccctaatta gctaacccgg gggcgcgccg ggacccaagc ttgatatcgg aagtttctct      240

cttgagggag gttgctcgtg gaatgggaca catatggttg ttataataaa ccatttccat      300

tgtcatgaga ttttgaggtt aatatatact ttacttgttc attattttat ttggtgtttg      360

aataaatgat ataaatggct cttgataatc tgcattcatt gagatatcaa atatttactc      420

tagagaagag tgtcatatag attgatggtc cacaatcaat gaaatttttg gaagacgaac      480

atgtataacc atttgcttga ataaccttaa ttaaaaggtg tgattaaatg atgtttgtaa      540

catgtagtac taaacattca taaaacacaa ccaacccaag aggtattgag tattcacggc      600

taaacagggg cataatggta atttaaagaa tgatattatt ttatgttaaa ccctaacatt      660

ggtttcggat tcaacgctat aaataaaacc actctcgttg ctgattccat ttatcgttct      720

tattgaccct agccgctaca cacttttctg cgatatctct gaggtaagcg ttaacgtacc      780

cttagatcgt tctttttctt tttcgtctgc tgatcgttgc tcatattatt tcgatgattg      840

ttggattcga tgctctttgt tgattgatcg ttctgaaaat tctgatctgt tgtttagatt      900

ttatcgattg ttaatatcaa cgtttcactg cttctaaacg ataatttatt catgaaacta      960

ttttcccatt ctgatcgatc ttgttttgag attttaattt gttcgattga ttgttggttg     1020

gtggatctat atacgagtga acttgttgat ttgcgtattt aagatgtatg tcgatttgaa     1080

ttgtgattgg gtaattctgg agtagcataa caaatccagt gttccctttt tctaagggta     1140

attctcggat tgtttgcttt atatctcttg aaattgccga tttgattgaa tttagctcgc     1200

ttagctcaga tgatagagca ccacaatttt tgtggtagaa atcggtttga ctccgatagc     1260

ggctttttac tatgattgtt ttgtgttaaa gatgattttc ataatggtta tatatctcta     1320

ctgtttttat tgattcaata tttgattgtt cttttttttg cagatttgtt gaccagggat     1380

cccctaaata gaccatggct attcagcaag ccactgcagg agtctcacaa gacacttcgg     1440

aaaatcctaa caaaacaata gttcctgctg cattaccaca gctcacccct accttggtgt     1500

cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc tctgttccag     1560

attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa gtgattgcag     1620

cagtgaaatg ggcaaaggcg ataccaggct tcagaaactt acacctggat gaccaaatga     1680

ccctgctaca gtactcatgg atgtttctca tggcatttgc cctgggttgg agatcataca     1740

gacaatcaag tggaaacctg ctctgctttg ctcctgatct gattattaat gagcagagaa     1800

tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc tctgaattac     1860

aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg cttctctcct     1920

cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga atgacttata     1980

tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag aactggcaac     2040

ggttttacca actgacaaag cttctggact ccatgcatga ggtggttgag aatctcctta     2100

cctactgctt ccagacattt ttggataaga ccatgagtat tgaattccca gagatgttag     2160

ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaaa aagcttctgt     2220

ttcatcaaaa agggggcggg ggctcaggcg ggggcgggag cggcggcggg ggctctgggg     2280

gcggcggcag cggcgggggc ggcagcgggg gcggcgggtc gatgtcaaag ctcgagaaat     2340

tcaccaactg ttattcgttg agcaaaacac tgcggtttaa agcgattcca gtcggcaaga     2400

ctcaagagaa tatagacaat aagcggctgt tggtggaaga tgaaaagcgc gcggaagact     2460

acaaaggggt gaagaagttg ttggacagat actacctctc ttttatcaat gatgtcttgc     2520

actcaatcaa attgaagaat ctgaacaact acatctccct cttcagaaag aaaacaagga     2580

cagaaaagga gaataaggaa cttgaaaatt tggagatcaa tctgaggaaa gagatcgcga     2640

aagcctttaa aggcaacgaa ggatacaaaa gtctgttcaa gaaggatata attgagacaa     2700

ttttgccaga gttcctcgat gacaaggacg agattgcgct ggtcaattcg ttcaacggat     2760

tcacaacagc attcacaggc ttctttgata atcgggaaaa tatgttctct gaggaggcaa     2820

agtccacttc tattgcgttc aggtgtatca atgagaatct cactaggtac atttccaaca     2880

tggatatctt tgagaaggtt gacgcaattt ttgacaagca cgaagttcag gagattaagg     2940

agaagatcct caattccgat tatgacgttg aggacttctt cgagggtgag ttttttaatt     3000

tcgtgctcac tcaagagggt atcgacgtgt ataatgcgat catcggtggg ttcgtgactg     3060

agtccggtga aaagattaag ggattgaacg agtatatcaa cctttacaac caaaagacga     3120

aacagaagct gccaaagttc aagcctcttt acaaacaggt tctttcagac cgcgagtcac     3180

tctcgttcta tggggagggc tacacttcgg atgaggaagt cctggaggtg ttcaggaata     3240

ctctcaataa gaattcggag attttctctt ctataaaaaa actggaaaag ttgtttaaga     3300

attttgacga atactctagc gccggcatat ttgtgaaaaa cggcccggcc atatcaacga     3360

taagtaaaga tatcttcggc gaatggaacg tgatcagaga caaatggaac gcggagtatg     3420

acgatattca cctgaagaag aaggctgtcg taacggagaa gtacgaggat gatcgcagga     3480

aaagcttcaa aaagatcgga agtttcagcc tggaacagtt gcaggagtat gctgacgccg     3540

atcttagcgt cgtcgagaag ttgaaggaga taatcatcca aaaggtcgac gagatatata     3600

aagtctatgg atcaagtgaa aaactgttcg acgccgactt cgttttggag aagtccctga     3660

agaagaacga cgctgttgtt gccattatga aggatctgct cgacagcgtg aagagtttcg     3720

agaactatat taaggctttt ttcggggagg ggaaggagac taacagagat gagtccttct     3780

acggagactt cgtcctcgcg tacgatatac tccttaaggt agaccacatc tacgacgcaa     3840

tcagaaatta cgtgacacaa aagccgtaca gcaaggacaa gttcaaactc tacttccaga     3900

acccccagtt catgggcggc tgggacaagg acaaggaaac ggattacagg gctacgatcc     3960

tgaggtatgg ttcaaaatac tacttggcga ttatggacaa gaagtacgcc aagtgtctcc     4020

agaagattga caaagacgat gtcaatggca attatgagaa gatcaactac aagctgcttc     4080

cgggtccgaa caagatgctc ccaaaggttt tcttcagcaa gaaatggatg gcctactata     4140

acccaagcga ggacatccag aagatttata agaacggtac gttcaagaag ggcgacatgt     4200

tcaatcttaa cgactgtcac aagctgatcg acttcttcaa agactcaatt agccggtacc     4260

caaagtggtc taacgcctat gacttcaact tttcggaaac cgagaagtac aaggatatag     4320

ccggatttta tagagaggtg gaagagcagg gctacaaggt gtcattcgag tccgccagca     4380

agaaggaagt ggacaagctc gtggaagagg gtaagctcta catgttccag atttataata     4440

aagactttag cgataagagc cacgggacac ctaatctcca cacaatgtat ttcaagctgc     4500

tcttcgacga gaataaccac ggccaaatca ggttgtcagg aggggctgaa ctcttcatgc     4560

ggcgcgctag ccttaagaag gaggagcttg tagtccaccc tgcgaatagt ccaattgcga     4620

ataagaaccc ggacaatcct aaaaagacta caacattgag ctacgacgtg tacaaggata     4680

agaggttttc cgaggatcag tacgagctcc acatcccgat tgcgatcaac aagtgcccaa     4740

agaatatttt caagataaac acagaggtgc gtgtactcct gaagcatgac gacaatcctt     4800

acgtcattgg gattgatcgg ggcgagagga acctcctcta tattgtggtg gtggacggga     4860

aggggaacat agtcgaacag tactccctta acgaaataat taacaatttc aacggcatcc     4920

gtatcaagac cgactaccat tcgttgctgg acaagaagga gaaggagaga tttgaggcgc     4980

ggcaaaattg gacaagtatc gagaacatca aggaactcaa agcaggttat atctctcaag     5040

ttgtgcataa gatatgcgag ctggttgaga agtatgacgc agtgatcgct cttgaggacc     5100

tcaactcggg ctttaagaat tctagagtta aagtggagaa gcaggtctat caaaagttcg     5160

agaagatgct tatagataag ctcaactaca tggtcgataa gaaatcgaac ccatgtgcca     5220

ccggcggcgc actcaaaggt taccaaataa caaacaaatt cgagtccttc aaatcgatga     5280

gtactcagaa tgggttcata ttttatatac cggcgtggct tacgtctaag atcgacccgt     5340

caactggttt tgtcaacctg ttgaagacga aatacacgtc cattgccgat tcaaaaaagt     5400

tcatatctag ttttgatcgt attatgtacg tcccagagga agatcttttc gagtttgctc     5460

tcgactacaa aaacttttcg cgcaccgatg cggattacat taaaaaatgg aaactctatt     5520

cgtacggcaa cagaatcagg atttttcgca accctaagaa gaataacgtc tttgattggg     5580

aggaagtttg cttgactagc gcgtacaagg agctctttaa taagtatggc attaactacc     5640

aacagggtga tatcagagca ctgctttgcg aacaatctga caaggctttc tactcatcct     5700

tcatggcttt gatgagcctg atgctccaga tgagaaattc aattacaggc agaaccgacg     5760

tggatttctt gatctccccg gttaaaaatt ctgatggcat cttttacgat agcaggaact     5820

atgaagcgca agagaatgcg attctgccaa aaaatgcaga cgccaacggt gcctataaca     5880

tcgccaggaa agtcctgtgg gcgatcggcc agttcaaaaa ggccgaagac gaaaaattgg     5940

acaaggtcaa aatcgctatc agcaacaaag agtggctgga gtatgctcag acatccgtaa     6000

agcattgatt aattaagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc     6060

tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat     6120

aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca     6180

attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc     6240

gcgcgcggtg tcatctatgt tactagatct tcgaattaac tagctagtcc accctcacaa     6300

aaaaggttat tgcctaatta atagtaggga ttgaagaagt atcaataatc aataagaggg     6360

acatttggat tgacacaaac tattagtata aaaaaaaaat gtaaaactaa atggacaaca     6420

atctaagaaa gcgagtatgt gttgtactcg gaaaacataa cataatgtga ttttgattgt     6480

ggaaactgaa aacaataaca attaagtttt atttatctct ttgcctaaca atttttttaa     6540

ttactttttt tatagttttt atgatgaaga taattaatat tttttacaaa tattatattt     6600

tcctttttac catctttgaa gataattgct tttttttcca atttagacaa atatttgagt     6660

atcaatgata ttccttttta atccattatt gcaatttaaa agaccatcaa tgattacatt     6720

ttcaatcaaa tgactttatt agtcttcact ttacatcgat ttaaatcaaa ctaataattt     6780

tgtatggact aaatctctga acatttttat atttacaaca tattttaaca tttattaaat     6840

aattagtatt taatactatt agtagaataa tgggagtagc aggagggagg cactgagaga     6900

atagagatgg catggaagta agcaatcaag tcaaaatcag agttggccaa ccccaaaggc     6960

tgtagtaggt aagcatggcc cattttagtt tttacattca tctctcattt tcacctcaac     7020

ggttcagatt caatctgact ccccgatctc agccgtggat tcaaatgcca cctcaggcac     7080

atgcaattcc aaatggatga acctaaccca caatctaatc ttgttactta ggggcttttc     7140

cgtcattaaa tgacaccacc tacccccttc tccctataaa tggcaactca atccccccct     7200

tagaactcgc agcgcttgat ttgaggccag gcaagcccca ctcaaccacc acacctctcc     7260

tcgttcacgc tacccctttc tgctcttctt ctacctttca aggtactctt ctttccctct     7320

gttgctgcaa ccttctcttt ctttaagatt gcctcaattt cggatcttgc acctctgggt     7380

tgctttgctt tgctttttcc tctactgggt tgatttctgt ttccctaaac cggtttagac     7440

gaatgtgaac actacttctt ttgtttaatt actctggaat acgtgttagg ctttcagatc     7500

tagttgaaat cgtattgcac ttttaggggg agtttggatt tctaataaga aattgacctt     7560

ttgctgagaa ttggttcggt gattagaggg tttccgtaaa tttttgaagt tttacatgct     7620

tgtatctgtt tatttttgtt tctcacatct attattgtta ggtgaaggaa attatgtatt     7680

gagagtctgt ctgatactaa atataaacac ctcaataggg gctctaacac tgattttatc     7740

atttgctgct tgtgtgtatg gttaaagaaa ggcaattgtg ttttaatttt ctgcaagctt     7800

tcgtttgctg aattttatgc atatattttc ctcccttttg tgaacttcct ttttgtagtt     7860

ctaattccat ttttggtgtc tgcagtttta aaagtataaa gggatccaaa ttactgatga     7920

gtccgtgagg acgaaacgag taagctcgtc taatttctac taagtgtaga tccacatctc     7980

tttagcccta tcaaggccgg catggtccca gcctcctcgc tggcgccggc tgggcaacat     8040

gcttcggcat ggcgaatggg acagcttttt gtgatctgat gataagtggt tggttcgtgt     8100

ctcatgcact tgggaggtga tctatttcac ctggtgtagt ttgtgtttcc gtcagttgga     8160

aaaacttatc cctatcgatt tcgttttcat tttctgcttt tcttttatgt accttcgttt     8220

gggcttgtaa cgggcctttg tatttcaact ctcaataata atccaagtgc atgttaaaca     8280

atttgtcatc tgtttcggct ttgatatact actggtgaag atgggccgta ctactgcatc     8340

acaacgaaaa ataataataa gatgaaaaac ttgaagtgga aaaaaaaaac ttgaatgttc     8400

actactacgg accgctagtg catgcactga ctaattagct aactggcaga caaagtggca     8460

gacatactgt cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt     8520

ctgacaaagg ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga     8580

aaaactgtgc agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt     8640

gggggtccac catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct     8700

tacgaaataa gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt     8760

agcccctccc tcattgttaa gggagcaaga cctaaaccat gattgaacaa gatggattgc     8820

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga     8880

caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt     8940

ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat     9000

cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg     9060

gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg     9120

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc     9180

cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga     9240

tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag     9300

ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc     9360

acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg     9420

actgtggccg gctgggtgtg gcagaccgct atcaggacat agcgttggct acccgtgata     9480

ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg     9540

ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgatgagagc     9600

tctagatccc cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg     9660

aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat     9720

gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc     9780

ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa     9840

ttatcgcgcg cggtgtcatc tatgttacta gatcattagc ctgcaggccc gggttagtct     9900

aattagctaa cggccaggat cgccgcgtga gcctttagca actagctaga gcgatcgccg     9960

caatctgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc    10020

agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag    10080

cccatcagaa ttaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc    10140

ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc    10200

ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca    10260

taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcc ggctcgtata    10320

atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaccatg agggaagcgt    10380

tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg    10440

aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac    10500

acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag    10560

ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg    10620

ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc    10680

gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag    10740

ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct    10800

tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg    10860

cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa    10920

atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga    10980

aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac    11040

ttgaagctag gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt    11100

tggaagaatt tgttcactac gtgaaaggcg agatcaccaa agtagtcggc aaataaagct    11160

ctagtggatc tccgtaccca gggatctggc tcgcggcgga cgcacgacgc cggggcgaga    11220

ccataggcga tctcctaaat caatagtagc tgtaacctcg aagcgtttca cttgtaacaa    11280

cgattgagaa tttttgtcat aaaattgaaa tacttggttc gcatttttgt catccgcggt    11340

cagccgcaat tctgacgaac tgcccattta gctggagatg attgtacatc cttcacgtga    11400

aaatttctca agcgctgtga acaagggttc agattttaga ttgaaaggtg agccgttgaa    11460

acacgttctt cttgtcgatg acgacgtcgc tatgcggcat cttattattg aataccttac    11520

gatccacgcc ttcaaagtga ccgcggtagc cgacagcacc cagttcacaa gagtactctc    11580

ttccgcgacg gtcgatgtcg tggttgttga tctagattta ggtcgtgaag atgggctcga    11640

gatcgttcgt aatctggcgg caaagtctga tattccaatc ataattatca gtggcgaccg    11700

ccttgaggag acagataaag ttgttgcact cgagctagga gcaagtgatt ttatcgctaa    11760

gccgttcagt atcagagagt ttctagcacg cattcgggtt gccttgcgcg tgcgccccaa    11820

cgttgtccgc tccaaagacc gacggtcttt ttgttttact gactggacac ttaatctcag    11880

gcaacgtcgc ttgatgtccg aagctggcgg tgaggtgaaa cttacggcag gtgagttcaa    11940

tcttctcctc gcgtttttag agaaaccccg cgacgttcta tcgcgcgagc aacttctcat    12000

tgccagtcga gtacgcgacg aggaggttta tgacaggagt atagatgttc tcattttgag    12060

gctgcgccgc aaacttgagg cagatccgtc aagccctcaa ctgataaaaa cagcaagagg    12120

tgccggttat ttctttgacg cggacgtgca ggtttcgcac ggggggacga tggcagcctg    12180

agccaattcc cagatccccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta    12240

caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc    12300

cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat    12360

cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg    12420

cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc    12480

gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct    12540

ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc    12600

ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa    12660

tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac    12720

gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg    12780

gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc    12840

aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc    12900

gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctggctga ttggatgtac    12960

cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg    13020

atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca    13080

gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag    13140

aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg    13200

aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag    13260

ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca    13320

ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag    13380

ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg    13440

tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct    13500

ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc    13560

acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc    13620

gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc    13680

aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg ctgaggtctg    13740

cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag    13800

aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg    13860

aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc    13920

aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc    13980

tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat    14040

gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct    14100

gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt    14160

ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa    14220

ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct    14280

ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc    14340

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct    14400

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg    14460

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc    14520

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga    14580

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct    14640

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg    14700

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag    14760

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat    14820

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac    14880

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac    14940

tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc    15000

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt    15060

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc    15120

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg    15180

agattatcaa aaaggatctt cacctagatc cttttgatcc ggaatta                  15227


<210>  3
<211>  13770
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vector 25765


<220>
<221>  promoter
<222>  (217)..(1376)
<223>  prAtEF1aA1

<220>
<221>  gene
<222>  (1395)..(3857)
<223>  AlcR Arabidopsis-optimized alcohol receptor gene (AlcR) from 
       Aspergillus nidulans

<220>
<221>  terminator
<222>  (3869)..(4121)
<223>  tNOS

<220>
<221>  promoter
<222>  (4128)..(4463)
<223>  prAlcA

<220>
<221>  exon
<222>  (4482)..(5096)
<223>  Gus

<220>
<221>  Intron
<222>  (5097)..(5505)
<223>  iAtBAF60

<220>
<221>  exon
<222>  (5506)..(6702)
<223>  Gus

<220>
<221>  terminator
<222>  (6711)..(6950)
<223>  tPI2

<400>  3
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt       60

taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc      120

tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct      180

tccctaatta gctaacccgg gggcgcgccg ggacccaagc ttgatatcgg aagtttctct      240

cttgagggag gttgctcgtg gaatgggaca catatggttg ttataataaa ccatttccat      300

tgtcatgaga ttttgaggtt aatatatact ttacttgttc attattttat ttggtgtttg      360

aataaatgat ataaatggct cttgataatc tgcattcatt gagatatcaa atatttactc      420

tagagaagag tgtcatatag attgatggtc cacaatcaat gaaatttttg gaagacgaac      480

atgtataacc atttgcttga ataaccttaa ttaaaaggtg tgattaaatg atgtttgtaa      540

catgtagtac taaacattca taaaacacaa ccaacccaag aggtattgag tattcacggc      600

taaacagggg cataatggta atttaaagaa tgatattatt ttatgttaaa ccctaacatt      660

ggtttcggat tcaacgctat aaataaaacc actctcgttg ctgattccat ttatcgttct      720

tattgaccct agccgctaca cacttttctg cgatatctct gaggtaagcg ttaacgtacc      780

cttagatcgt tctttttctt tttcgtctgc tgatcgttgc tcatattatt tcgatgattg      840

ttggattcga tgctctttgt tgattgatcg ttctgaaaat tctgatctgt tgtttagatt      900

ttatcgattg ttaatatcaa cgtttcactg cttctaaacg ataatttatt catgaaacta      960

ttttcccatt ctgatcgatc ttgttttgag attttaattt gttcgattga ttgttggttg     1020

gtggatctat atacgagtga acttgttgat ttgcgtattt aagatgtatg tcgatttgaa     1080

ttgtgattgg gtaattctgg agtagcataa caaatccagt gttccctttt tctaagggta     1140

attctcggat tgtttgcttt atatctcttg aaattgccga tttgattgaa tttagctcgc     1200

ttagctcaga tgatagagca ccacaatttt tgtggtagaa atcggtttga ctccgatagc     1260

ggctttttac tatgattgtt ttgtgttaaa gatgattttc ataatggtta tatatctcta     1320

ctgtttttat tgattcaata tttgattgtt cttttttttg cagatttgtt gaccagggat     1380

cccctaaata gaccatggct gatactcgaa gaagacagaa tcattcttgt gatccatgcc     1440

gcaaaggtaa acgtcggtgt gacgcacctg aaaacagaaa tgaagcaaac gagaatggat     1500

gggtgagctg ttcaaactgt aagagatgga acaaagattg cactttcaac tggttatcgt     1560

cccagagaag caaggcgaaa ggtgcagctc cacgggctcg aaccaagaaa gctagaactg     1620

ccaccacgac atctgaacca tcaacatctg cagcaaccat tccaacacca gagtcggaca     1680

accatgacgc tcctccggtg ataaactctc acgatgccct ccctagttgg acccagggac     1740

tattatcgca tcctggggac ctttttgact tttctcatag tgccattccg gcgaatgcag     1800

aagatgcagc caatgttcaa tcggatgctc catttccatg ggatcttgca attccaggtg     1860

atttctcaat gggacagcag cttgagaaac ctctctcccc tttgtcattc caagctgtcc     1920

tattacctcc tcattcccca aacacagatg atcttataag agagctagaa gagcaaacca     1980

ctgatcctga ttcagttacg gacacaaaca gtgtgcaaca agttgctcaa gatggatcct     2040

tgtggtcaga caggcaaagt ccgttactac ctgaaaattc tctctgcatg gcttcggact     2100

caaccgctcg tagatatgca cggagtacaa tgacaaaaaa cttgatgcgt atctaccatg     2160

attccatgga gaacgctctt tcttgctggc tcactgaaca caattgtcct tattctgatc     2220

aaatctctta tctcccacct aagcaaaggg ctgaatgggg tcctaattgg agcaatagga     2280

tgtgtataag ggtttgtaga cttgatcgtg taagtacgtc actgagaggc agagcattat     2340

cagcagaaga agacaaagct gctgctagag cccttcatct ggcgattgtt gcattcgcat     2400

ctcaatggac gcaacacgct caacgcggag ctggtttgaa tgtcccggct gacattgctg     2460

ccgacgaaag atcaatcagg agaaacgctt ggaatgaggc aagacacgcg ttgcagcata     2520

ctactgggat cccttctttc cgagtgattt tcgctaatat catcttcagc ctgactcaga     2580

gtgtactgga tgatgatgaa caacatggaa tgggtgcgcg attggacaag cttttggaga     2640

atgatggtgc accggttttc ttggaaactg ctaataggca attgtacaca tttcgtcaca     2700

agtttgccag aatgcaacgg agaggcaagg cttttaatcg cttacccggt ggttccgtcg     2760

cttccacttt tgctggaata tttgagaccc caactccatc tagtgagtct cctcaacttg     2820

atccagttgt tgcatctgag gaacatcgta gcactctatc gctcatgttc tggcttggaa     2880

taatgtttga tacactttca gctgcaatgt accagagacc attagtggta tctgatgagg     2940

attcacagat atccagtgct tctcccccga gacgaggcgc cgagactcct atcaatttag     3000

actgttggga acctccacgt caagtccctt cgaatcaaga aaagtctgat gtttggggag     3060

atttgtttct cagaacatca gactctttac cagatcatga atcacacaca cagatttctc     3120

agccagctgc aaggtggcct tgcacctatg agcaggctgc ggccgcattg agctcagcga     3180

cacctgttaa ggttttgctc tacaggagag tgactcaact gcaaacactt ctatacaggg     3240

gggcgtctcc agcgaggctt gaagctgcga tccaaagaac cctttacgtg tataatcact     3300

ggacagctaa gtatcaacct ttcatgcagg attgtgttgc aaatcatgag ttgttgccca     3360

gccggattca gtcatggtac gtcatccttg acggccattg gcatcttgct gctatgttgc     3420

ttgctgatgt gttagaaagc atagatagag attcgtatag cgatataaac cacattgatt     3480

tggttacgaa gttgcgtctc gataatgctc tagccgtctc tgcccttgcg agatcttcct     3540

tgcgtggtca agagcttgat ccggggaaag catcaccaat gtataggcat ttccatgata     3600

gtctaacgga agtggctttt cttgtcgagc cgtggactgt agtacttatt cactcttttg     3660

caaaggcagc ttatattctc ttagattgtt tggatctcga cggacaagga aacgcactgg     3720

cgggctacct gcagctccgt cagaactgca actattgcat ccgagctctt cagtttttag     3780

gtagaaaatc cgatatggca gctctagtag ctaaagacct cgagaggggt ttaaacggaa     3840

aagttgatag ctttctgtga ttaattaaga tcgttcaaac atttggcaat aaagtttctt     3900

aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt     3960

taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat     4020

tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta     4080

ggataaatta tcgcgcgcgg tgtcatctat gttactagat ctccggatcg atagttgtga     4140

tagttcccac ttgtccgtcc gcatcggcat ccgcagctcg ggatagttcc gacctaggat     4200

tggatgcatg cggaaccgca cgagggcggg gcggaaattg acacaccact cctctccacg     4260

caccgttcaa gaggtacgcg tatagagccg tatagagcag agacggagca ctttctggta     4320

ctgtccgcac gggatgtccg cacggagagc cacaaacgag cggggccccg tacgtgctct     4380

cctaccccag gatcgcatcc ccgcatagct gaacatctat ataaagaccc ccaaggttct     4440

cagtctcacc aacatcatca accggatccc ctaaatagac c atg gta cgt cct gta     4496
                                              Met Val Arg Pro Val         
                                              1               5           

gaa acc cca acc cgt gaa atc aaa aaa ctc gac ggc ctg tgg gca ttc       4544
Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp Gly Leu Trp Ala Phe           
                10                  15                  20                

agt ctg gat cgc gaa aac tgt gga att gat cag cgt tgg tgg gaa agc       4592
Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln Arg Trp Trp Glu Ser           
            25                  30                  35                    

gcg tta caa gaa agc cgg gca att gct gtg cca ggc agt ttt aac gat       4640
Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro Gly Ser Phe Asn Asp           
        40                  45                  50                        

cag ttc gcc gat gca gat att cgt aat tat gcg ggc aac gtc tgg tat       4688
Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala Gly Asn Val Trp Tyr           
    55                  60                  65                            

cag cgc gaa gtc ttt ata ccg aaa ggt tgg gca ggc cag cgt atc gtg       4736
Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala Gly Gln Arg Ile Val           
70                  75                  80                  85            

ctg cgt ttc gat gcg gtc act cat tac ggc aaa gtg tgg gtc aat aat       4784
Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys Val Trp Val Asn Asn           
                90                  95                  100               

cag gaa gtg atg gag cat cag ggc ggc tat acg cca ttt gaa gcc gat       4832
Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr Pro Phe Glu Ala Asp           
            105                 110                 115                   

gtc acg ccg tat gtt att gcc ggg aaa agt gta cgt atc acc gtt tgt       4880
Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val Arg Ile Thr Val Cys           
        120                 125                 130                       

gtg aac aac gaa ctg aac tgg cag act atc ccg ccg gga atg gtg att       4928
Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro Pro Gly Met Val Ile           
    135                 140                 145                           

acc gac gaa aac ggc aag aaa aag cag tct tac ttc cat gat ttc ttt       4976
Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr Phe His Asp Phe Phe           
150                 155                 160                 165           

aac tat gcc gga atc cat cgc agc gta atg ctc tac acc acg ccg aac       5024
Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu Tyr Thr Thr Pro Asn           
                170                 175                 180               

acc tgg gtg gac gat atc acc gtg gtg acg cat gtc gcg caa gac tgt       5072
Thr Trp Val Asp Asp Ile Thr Val Val Thr His Val Ala Gln Asp Cys           
            185                 190                 195                   

aac cac gcg tct gtt gac tgg cag gtaccaagct gcgaatcttc gtttttttaa      5126
Asn His Ala Ser Val Asp Trp Gln                                           
        200                 205                                           

ggaattctcg atctttatgg tgtataggct ctgggttttc tgttttttgt atctcttagg     5186

attttgtaaa ttccagatct ttctatggcc acttagtagt atatttcaaa aattctccaa     5246

tcgagttctt cattcgcatt ttcagtcatt ttctcttcga cgttgttttt aagcctgggt     5306

attactccta tttagttgaa ctctgcagca atcttagaaa attagggttt tgaggtttcg     5366

atttctctag gtaaccgatc tattgcattc atctgaattt ctgcatatat gtcttagatt     5426

tctgataagc ttacgatacg ttaggtgtaa ttgaagttta tttttcaaga gtgttatttt     5486

ttgtttctga atttttcag gtg gtg gcc aat ggt gat gtc agc gtt gaa ctg      5538
                     Val Val Ala Asn Gly Asp Val Ser Val Glu Leu          
                                     210                 215              

cgt gat gcg gat caa cag gtg gtt gca act gga caa ggc act agc ggg       5586
Arg Asp Ala Asp Gln Gln Val Val Ala Thr Gly Gln Gly Thr Ser Gly           
            220                 225                 230                   

act ttg caa gtg gtg aat ccg cac ctc tgg caa ccg ggt gaa ggt tat       5634
Thr Leu Gln Val Val Asn Pro His Leu Trp Gln Pro Gly Glu Gly Tyr           
        235                 240                 245                       

ctc tat gaa ctg tgc gtc aca gcc aaa agc cag aca gag tgt gat atc       5682
Leu Tyr Glu Leu Cys Val Thr Ala Lys Ser Gln Thr Glu Cys Asp Ile           
    250                 255                 260                           

tac ccg ctt cgc gtc ggc atc cgg tca gtg gca gtg aag ggc gaa cag       5730
Tyr Pro Leu Arg Val Gly Ile Arg Ser Val Ala Val Lys Gly Glu Gln           
265                 270                 275                 280           

ttc ctg att aac cac aaa ccg ttc tac ttt act ggc ttt ggt cgt cat       5778
Phe Leu Ile Asn His Lys Pro Phe Tyr Phe Thr Gly Phe Gly Arg His           
                285                 290                 295               

gaa gat gcg gac ttg cgt ggc aaa gga ttc gat aac gtg ctg atg gtg       5826
Glu Asp Ala Asp Leu Arg Gly Lys Gly Phe Asp Asn Val Leu Met Val           
            300                 305                 310                   

cac gac cac gca tta atg gac tgg att ggg gcc aac tcc tac cgt acc       5874
His Asp His Ala Leu Met Asp Trp Ile Gly Ala Asn Ser Tyr Arg Thr           
        315                 320                 325                       

tcg cat tac cct tac gct gaa gag atg ctc gac tgg gca gat gaa cat       5922
Ser His Tyr Pro Tyr Ala Glu Glu Met Leu Asp Trp Ala Asp Glu His           
    330                 335                 340                           

ggc atc gtg gtg att gat gaa act gct gct gtc ggc ttt aac ctc tct       5970
Gly Ile Val Val Ile Asp Glu Thr Ala Ala Val Gly Phe Asn Leu Ser           
345                 350                 355                 360           

tta ggc att ggt ttc gaa gcg ggc aac aag ccg aaa gaa ctg tac agc       6018
Leu Gly Ile Gly Phe Glu Ala Gly Asn Lys Pro Lys Glu Leu Tyr Ser           
                365                 370                 375               

gaa gag gca gtc aac ggg gaa act cag caa gcg cac tta cag gcg att       6066
Glu Glu Ala Val Asn Gly Glu Thr Gln Gln Ala His Leu Gln Ala Ile           
            380                 385                 390                   

aaa gag ctg ata gcg cgt gac aaa aac cac cca agc gtg gtg atg tgg       6114
Lys Glu Leu Ile Ala Arg Asp Lys Asn His Pro Ser Val Val Met Trp           
        395                 400                 405                       

agt att gcc aac gaa ccg gat acc cgt ccg caa ggt gca cgg gaa tat       6162
Ser Ile Ala Asn Glu Pro Asp Thr Arg Pro Gln Gly Ala Arg Glu Tyr           
    410                 415                 420                           

ttc gcg cca ctg gcg gaa gca acg cgt aaa ctc gac ccg acg cgt ccg       6210
Phe Ala Pro Leu Ala Glu Ala Thr Arg Lys Leu Asp Pro Thr Arg Pro           
425                 430                 435                 440           

atc acc tgc gtc aat gta atg ttc tgc gac gct cac acc gat acc atc       6258
Ile Thr Cys Val Asn Val Met Phe Cys Asp Ala His Thr Asp Thr Ile           
                445                 450                 455               

agc gat ctc ttt gat gtg ctg tgc ctg aac cgt tat tac gga tgg tat       6306
Ser Asp Leu Phe Asp Val Leu Cys Leu Asn Arg Tyr Tyr Gly Trp Tyr           
            460                 465                 470                   

gtc caa agc ggc gat ttg gaa acg gca gag aag gta ctg gaa aaa gaa       6354
Val Gln Ser Gly Asp Leu Glu Thr Ala Glu Lys Val Leu Glu Lys Glu           
        475                 480                 485                       

ctt ctg gcc tgg cag gag aaa ctg cat cag ccg att atc atc acc gaa       6402
Leu Leu Ala Trp Gln Glu Lys Leu His Gln Pro Ile Ile Ile Thr Glu           
    490                 495                 500                           

tac ggc gtg gat acg tta gcc ggg ctg cac tca atg tac acc gac atg       6450
Tyr Gly Val Asp Thr Leu Ala Gly Leu His Ser Met Tyr Thr Asp Met           
505                 510                 515                 520           

tgg agt gaa gag tat cag tgt gca tgg ctg gat atg tat cac cgc gtc       6498
Trp Ser Glu Glu Tyr Gln Cys Ala Trp Leu Asp Met Tyr His Arg Val           
                525                 530                 535               

ttt gat cgc gtc agc gcc gtc gtc ggt gaa cag gta tgg aat ttc gcc       6546
Phe Asp Arg Val Ser Ala Val Val Gly Glu Gln Val Trp Asn Phe Ala           
            540                 545                 550                   

gat ttt gcg acc tcg caa ggc ata ttg cgc gtt ggc ggt aac aag aaa       6594
Asp Phe Ala Thr Ser Gln Gly Ile Leu Arg Val Gly Gly Asn Lys Lys           
        555                 560                 565                       

ggg atc ttc act cgc gac cgc aaa ccg aag tcg gcg gct ttt ctg ctg       6642
Gly Ile Phe Thr Arg Asp Arg Lys Pro Lys Ser Ala Ala Phe Leu Leu           
    570                 575                 580                           

caa aaa cgc tgg act ggc atg aac ttc ggt gaa aaa ccg cag cag gga       6690
Gln Lys Arg Trp Thr Gly Met Asn Phe Gly Glu Lys Pro Gln Gln Gly           
585                 590                 595                 600           

ggc aaa caa tga ttaattaaga ccctagactt gtccatcttc tggattggcc           6742
Gly Lys Gln                                                               
                                                                          

aacttaatta atgtatgaaa taaaaggatg cacacatagt gacatgctaa tcactataat     6802

gtgggcatca aagttgtgtg ttatgtgtaa ttactaatta tctgaataag agaaagagat     6862

catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag     6922

atgcatttta ttaaccaatt ccatataccg gaccgctagt gcatgcactg actaattagc     6982

taactggcag acaaagtggc agacatactg tcccacaaat gaagatggaa tctgtaaaag     7042

aaaacgcgtg aaataatgcg tctgacaaag gttaggtcgg ctgcctttaa tcaataccaa     7102

agtggtccct accacgatgg aaaaactgtg cagtcggttt ggctttttct gacgaacaaa     7162

taagattcgt ggccgacagg tgggggtcca ccatgtgaag gcatcttcag actccaataa     7222

tggagcaatg acgtaagggc ttacgaaata agtaagggta gtttgggaaa tgtccactca     7282

cccgtcagtc tataaatact tagcccctcc ctcattgtta agggagcaag acctaaacca     7342

tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg     7402

gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag     7462

cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc     7522

aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc     7582

tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg     7642

atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc     7702

ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca     7762

tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag     7822

agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg     7882

gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg     7942

gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcagaccgc tatcaggaca     8002

tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc     8062

tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg     8122

acgagttctt ctgatgagag ctctagatcc ccgaatttcc ccgatcgttc aaacatttgg     8182

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt     8242

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga     8302

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata     8362

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcattag     8422

cctgcaggcc cgggttagtc taattagcta acggccagga tcgccgcgtg agcctttagc     8482

aactagctag agcgatcgcc gcaatctgtt attaagttgt ctaagcgtca atttgtttac     8542

accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa     8602

atcaccactc gatacaggca gcccatcaga attaattctc atgtttgaca gcttatcatc     8662

gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct     8722

gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata     8782

atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca     8842

attaatcatc cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga     8902

aacagaccat gagggaagcg ttgatcgccg aagtatcgac tcaactatca gaggtagttg     8962

gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag     9022

tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc     9082

ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg     9142

gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc     9202

cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc     9262

ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag     9322

caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc     9382

ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg     9442

actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag     9502

taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg     9562

cccagtatca gcccgtcata cttgaagcta ggcaggctta tcttggacaa gaagatcgct     9622

tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca     9682

aagtagtcgg caaataaagc tctagtggat ctccgtaccc agggatctgg ctcgcggcgg     9742

acgcacgacg ccggggcgag accataggcg atctcctaaa tcaatagtag ctgtaacctc     9802

gaagcgtttc acttgtaaca acgattgaga atttttgtca taaaattgaa atacttggtt     9862

cgcatttttg tcatccgcgg tcagccgcaa ttctgacgaa ctgcccattt agctggagat     9922

gattgtacat ccttcacgtg aaaatttctc aagcgctgtg aacaagggtt cagattttag     9982

attgaaaggt gagccgttga aacacgttct tcttgtcgat gacgacgtcg ctatgcggca    10042

tcttattatt gaatacctta cgatccacgc cttcaaagtg accgcggtag ccgacagcac    10102

ccagttcaca agagtactct cttccgcgac ggtcgatgtc gtggttgttg atctagattt    10162

aggtcgtgaa gatgggctcg agatcgttcg taatctggcg gcaaagtctg atattccaat    10222

cataattatc agtggcgacc gccttgagga gacagataaa gttgttgcac tcgagctagg    10282

agcaagtgat tttatcgcta agccgttcag tatcagagag tttctagcac gcattcgggt    10342

tgccttgcgc gtgcgcccca acgttgtccg ctccaaagac cgacggtctt tttgttttac    10402

tgactggaca cttaatctca ggcaacgtcg cttgatgtcc gaagctggcg gtgaggtgaa    10462

acttacggca ggtgagttca atcttctcct cgcgttttta gagaaacccc gcgacgttct    10522

atcgcgcgag caacttctca ttgccagtcg agtacgcgac gaggaggttt atgacaggag    10582

tatagatgtt ctcattttga ggctgcgccg caaacttgag gcagatccgt caagccctca    10642

actgataaaa acagcaagag gtgccggtta tttctttgac gcggacgtgc aggtttcgca    10702

cggggggacg atggcagcct gagccaattc ccagatcccc gaggaatcgg cgtgagcggt    10762

cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag    10822

ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa    10882

tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt    10942

gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg    11002

ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg    11062

tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta    11122

gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg    11182

gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc    11242

ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc    11302

ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc    11362

atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc    11422

ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc    11482

gagctggctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg    11542

gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca    11602

cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt    11662

ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat    11722

gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg    11782

cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg    11842

ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc    11902

acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca    11962

aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc    12022

gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt    12082

gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg    12142

ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa    12202

atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc    12262

gaccgccggc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga    12322

atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag    12382

gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga    12442

agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt    12502

cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga    12562

aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat    12622

atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga    12682

tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta    12742

atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat    12802

ccggtgagaa tggcaaaagc tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt    12862

ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg    12922

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg    12982

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag    13042

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga    13102

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct    13162

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc    13222

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg    13282

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc    13342

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca    13402

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag    13462

ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct    13522

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc    13582

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga    13642

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca    13702

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgatc    13762

cggaatta                                                             13770


<210>  4
<211>  17492
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vector 25881


<220>
<221>  promoter
<222>  (217)..(1376)
<223>  prAtEF1aA1

<220>
<221>  gene
<222>  (1395)..(3860)
<223>  AlcR transcription factor from Aspergillus nidulans optimized for
       expression in dicots.

<220>
<221>  terminator
<222>  (3869)..(4121)
<223>  tNOS

<220>
<221>  promoter
<222>  (4128)..(4463)
<223>  prAlcA

<220>
<221>  gene
<222>  (4482)..(8264)
<223>  LbCas12a This Cas12a is a rice codon-optimized version from 
       Lachnospiraceae bacterium ND2006

<220>
<221>  terminator
<222>  (8272)..(8511)
<223>  tPI2

<220>
<221>  promoter
<222>  (8550)..(10163)
<223>  prGmSAMS

<220>
<221>  misc_feature
<222>  (10176)..(10212)
<223>  Hammerhead ribozyme

<220>
<221>  misc_feature
<222>  (10213)..(10256)
<223>  LbCas12a guide RNA targeting AtGl1

<220>
<221>  misc_feature
<222>  (10257)..(10324)
<223>  HDV ribozyme

<400>  4
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt       60

taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc      120

tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct      180

tccctaatta gctaacccgg gggcgcgccg ggacccaagc ttgatatcgg aagtttctct      240

cttgagggag gttgctcgtg gaatgggaca catatggttg ttataataaa ccatttccat      300

tgtcatgaga ttttgaggtt aatatatact ttacttgttc attattttat ttggtgtttg      360

aataaatgat ataaatggct cttgataatc tgcattcatt gagatatcaa atatttactc      420

tagagaagag tgtcatatag attgatggtc cacaatcaat gaaatttttg gaagacgaac      480

atgtataacc atttgcttga ataaccttaa ttaaaaggtg tgattaaatg atgtttgtaa      540

catgtagtac taaacattca taaaacacaa ccaacccaag aggtattgag tattcacggc      600

taaacagggg cataatggta atttaaagaa tgatattatt ttatgttaaa ccctaacatt      660

ggtttcggat tcaacgctat aaataaaacc actctcgttg ctgattccat ttatcgttct      720

tattgaccct agccgctaca cacttttctg cgatatctct gaggtaagcg ttaacgtacc      780

cttagatcgt tctttttctt tttcgtctgc tgatcgttgc tcatattatt tcgatgattg      840

ttggattcga tgctctttgt tgattgatcg ttctgaaaat tctgatctgt tgtttagatt      900

ttatcgattg ttaatatcaa cgtttcactg cttctaaacg ataatttatt catgaaacta      960

ttttcccatt ctgatcgatc ttgttttgag attttaattt gttcgattga ttgttggttg     1020

gtggatctat atacgagtga acttgttgat ttgcgtattt aagatgtatg tcgatttgaa     1080

ttgtgattgg gtaattctgg agtagcataa caaatccagt gttccctttt tctaagggta     1140

attctcggat tgtttgcttt atatctcttg aaattgccga tttgattgaa tttagctcgc     1200

ttagctcaga tgatagagca ccacaatttt tgtggtagaa atcggtttga ctccgatagc     1260

ggctttttac tatgattgtt ttgtgttaaa gatgattttc ataatggtta tatatctcta     1320

ctgtttttat tgattcaata tttgattgtt cttttttttg cagatttgtt gaccagggat     1380

cccctaaata gaccatggct gatactcgaa gaagacagaa tcattcttgt gatccatgcc     1440

gcaaaggtaa acgtcggtgt gacgcacctg aaaacagaaa tgaagcaaac gagaatggat     1500

gggtgagctg ttcaaactgt aagagatgga acaaagattg cactttcaac tggttatcgt     1560

cccagagaag caaggcgaaa ggtgcagctc cacgggctcg aaccaagaaa gctagaactg     1620

ccaccacgac atctgaacca tcaacatctg cagcaaccat tccaacacca gagtcggaca     1680

accatgacgc tcctccggtg ataaactctc acgatgccct ccctagttgg acccagggac     1740

tattatcgca tcctggggac ctttttgact tttctcatag tgccattccg gcgaatgcag     1800

aagatgcagc caatgttcaa tcggatgctc catttccatg ggatcttgca attccaggtg     1860

atttctcaat gggacagcag cttgagaaac ctctctcccc tttgtcattc caagctgtcc     1920

tattacctcc tcattcccca aacacagatg atcttataag agagctagaa gagcaaacca     1980

ctgatcctga ttcagttacg gacacaaaca gtgtgcaaca agttgctcaa gatggatcct     2040

tgtggtcaga caggcaaagt ccgttactac ctgaaaattc tctctgcatg gcttcggact     2100

caaccgctcg tagatatgca cggagtacaa tgacaaaaaa cttgatgcgt atctaccatg     2160

attccatgga gaacgctctt tcttgctggc tcactgaaca caattgtcct tattctgatc     2220

aaatctctta tctcccacct aagcaaaggg ctgaatgggg tcctaattgg agcaatagga     2280

tgtgtataag ggtttgtaga cttgatcgtg taagtacgtc actgagaggc agagcattat     2340

cagcagaaga agacaaagct gctgctagag cccttcatct ggcgattgtt gcattcgcat     2400

ctcaatggac gcaacacgct caacgcggag ctggtttgaa tgtcccggct gacattgctg     2460

ccgacgaaag atcaatcagg agaaacgctt ggaatgaggc aagacacgcg ttgcagcata     2520

ctactgggat cccttctttc cgagtgattt tcgctaatat catcttcagc ctgactcaga     2580

gtgtactgga tgatgatgaa caacatggaa tgggtgcgcg attggacaag cttttggaga     2640

atgatggtgc accggttttc ttggaaactg ctaataggca attgtacaca tttcgtcaca     2700

agtttgccag aatgcaacgg agaggcaagg cttttaatcg cttacccggt ggttccgtcg     2760

cttccacttt tgctggaata tttgagaccc caactccatc tagtgagtct cctcaacttg     2820

atccagttgt tgcatctgag gaacatcgta gcactctatc gctcatgttc tggcttggaa     2880

taatgtttga tacactttca gctgcaatgt accagagacc attagtggta tctgatgagg     2940

attcacagat atccagtgct tctcccccga gacgaggcgc cgagactcct atcaatttag     3000

actgttggga acctccacgt caagtccctt cgaatcaaga aaagtctgat gtttggggag     3060

atttgtttct cagaacatca gactctttac cagatcatga atcacacaca cagatttctc     3120

agccagctgc aaggtggcct tgcacctatg agcaggctgc ggccgcattg agctcagcga     3180

cacctgttaa ggttttgctc tacaggagag tgactcaact gcaaacactt ctatacaggg     3240

gggcgtctcc agcgaggctt gaagctgcga tccaaagaac cctttacgtg tataatcact     3300

ggacagctaa gtatcaacct ttcatgcagg attgtgttgc aaatcatgag ttgttgccca     3360

gccggattca gtcatggtac gtcatccttg acggccattg gcatcttgct gctatgttgc     3420

ttgctgatgt gttagaaagc atagatagag attcgtatag cgatataaac cacattgatt     3480

tggttacgaa gttgcgtctc gataatgctc tagccgtctc tgcccttgcg agatcttcct     3540

tgcgtggtca agagcttgat ccggggaaag catcaccaat gtataggcat ttccatgata     3600

gtctaacgga agtggctttt cttgtcgagc cgtggactgt agtacttatt cactcttttg     3660

caaaggcagc ttatattctc ttagattgtt tggatctcga cggacaagga aacgcactgg     3720

cgggctacct gcagctccgt cagaactgca actattgcat ccgagctctt cagtttttag     3780

gtagaaaatc cgatatggca gctctagtag ctaaagacct cgagaggggt ttaaacggaa     3840

aagttgatag ctttctgtga ttaattaaga tcgttcaaac atttggcaat aaagtttctt     3900

aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt     3960

taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat     4020

tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta     4080

ggataaatta tcgcgcgcgg tgtcatctat gttactagat ctccggatcg atagttgtga     4140

tagttcccac ttgtccgtcc gcatcggcat ccgcagctcg ggatagttcc gacctaggat     4200

tggatgcatg cggaaccgca cgagggcggg gcggaaattg acacaccact cctctccacg     4260

caccgttcaa gaggtacgcg tatagagccg tatagagcag agacggagca ctttctggta     4320

ctgtccgcac gggatgtccg cacggagagc cacaaacgag cggggccccg tacgtgctct     4380

cctaccccag gatcgcatcc ccgcatagct gaacatctat ataaagaccc ccaaggttct     4440

cagtctcacc aacatcatca accggatccc ctaaatagac catggctcct aagaagaagc     4500

ggaaggttgg tattcacggg gtgcctgcgg cttcaaagct cgagaaattc accaactgtt     4560

attcgttgag caaaacactg cggtttaaag cgattccagt cggcaagact caagagaata     4620

tagacaataa gcggctgttg gtggaagatg aaaagcgcgc ggaagactac aaaggggtga     4680

agaagttgtt ggacagatac tacctctctt ttatcaatga tgtcttgcac tcaatcaaat     4740

tgaagaatct gaacaactac atctccctct tcagaaagaa aacaaggaca gaaaaggaga     4800

ataaggaact tgaaaatttg gagatcaatc tgaggaaaga gatcgcgaaa gcctttaaag     4860

gcaacgaagg atacaaaagt ctgttcaaga aggatataat tgagacaatt ttgccagagt     4920

tcctcgatga caaggacgag attgcgctgg tcaattcgtt caacggattc acaacagcat     4980

tcacaggctt ctttgataat cgggaaaata tgttctctga ggaggcaaag tccacttcta     5040

ttgcgttcag gtgtatcaat gagaatctca ctaggtacat ttccaacatg gatatctttg     5100

agaaggttga cgcaattttt gacaagcacg aagttcagga gattaaggag aagatcctca     5160

attccgatta tgacgttgag gacttcttcg agggtgagtt ttttaatttc gtgctcactc     5220

aagagggtat cgacgtgtat aatgcgatca tcggtgggtt cgtgactgag tccggtgaaa     5280

agattaaggg attgaacgag tatatcaacc tttacaacca aaagacgaaa cagaagctgc     5340

caaagttcaa gcctctttac aaacaggttc tttcagaccg cgagtcactc tcgttctatg     5400

gggagggcta cacttcggat gaggaagtcc tggaggtgtt caggaatact ctcaataaga     5460

attcggagat tttctcttct ataaaaaaac tggaaaagtt gtttaagaat tttgacgaat     5520

actctagcgc cggcatattt gtgaaaaacg gcccggccat atcaacgata agtaaagata     5580

tcttcggcga atggaacgtg atcagagaca aatggaacgc ggagtatgac gatattcacc     5640

tgaagaagaa ggctgtcgta acggagaagt acgaggatga tcgcaggaaa agcttcaaaa     5700

agatcggaag tttcagcctg gaacagttgc aggagtatgc tgacgccgat cttagcgtcg     5760

tcgagaagtt gaaggagata atcatccaaa aggtcgacga gatatataaa gtctatggat     5820

caagtgaaaa actgttcgac gccgacttcg ttttggagaa gtccctgaag aagaacgacg     5880

ctgttgttgc cattatgaag gatctgctcg acagcgtgaa gagtttcgag aactatatta     5940

aggctttttt cggggagggg aaggagacta acagagatga gtccttctac ggagacttcg     6000

tcctcgcgta cgatatactc cttaaggtag accacatcta cgacgcaatc agaaattacg     6060

tgacacaaaa gccgtacagc aaggacaagt tcaaactcta cttccagaac ccccagttca     6120

tgggcggctg ggacaaggac aaggaaacgg attacagggc tacgatcctg aggtatggtt     6180

caaaatacta cttggcgatt atggacaaga agtacgccaa gtgtctccag aagattgaca     6240

aagacgatgt caatggcaat tatgagaaga tcaactacaa gctgcttccg ggtccgaaca     6300

agatgctccc aaaggttttc ttcagcaaga aatggatggc ctactataac ccaagcgagg     6360

acatccagaa gatttataag aacggtacgt tcaagaaggg cgacatgttc aatcttaacg     6420

actgtcacaa gctgatcgac ttcttcaaag actcaattag ccggtaccca aagtggtcta     6480

acgcctatga cttcaacttt tcggaaaccg agaagtacaa ggatatagcc ggattttata     6540

gagaggtgga agagcagggc tacaaggtgt cattcgagtc cgccagcaag aaggaagtgg     6600

acaagctcgt ggaagagggt aagctctaca tgttccagat ttataataaa gactttagcg     6660

ataagagcca cgggacacct aatctccaca caatgtattt caagctgctc ttcgacgaga     6720

ataaccacgg ccaaatcagg ttgtcaggag gggctgaact cttcatgcgg cgcgctagcc     6780

ttaagaagga ggagcttgta gtccaccctg cgaatagtcc aattgcgaat aagaacccgg     6840

acaatcctaa aaagactaca acattgagct acgacgtgta caaggataag aggttttccg     6900

aggatcagta cgagctccac atcccgattg cgatcaacaa gtgcccaaag aatattttca     6960

agataaacac agaggtgcgt gtactcctga agcatgacga caatccttac gtcattggga     7020

ttgatcgggg cgagaggaac ctcctctata ttgtggtggt ggacgggaag gggaacatag     7080

tcgaacagta ctcccttaac gaaataatta acaatttcaa cggcatccgt atcaagaccg     7140

actaccattc gttgctggac aagaaggaga aggagagatt tgaggcgcgg caaaattgga     7200

caagtatcga gaacatcaag gaactcaaag caggttatat ctctcaagtt gtgcataaga     7260

tatgcgagct ggttgagaag tatgacgcag tgatcgctct tgaggacctc aactcgggct     7320

ttaagaattc tagagttaaa gtggagaagc aggtctatca aaagttcgag aagatgctta     7380

tagataagct caactacatg gtcgataaga aatcgaaccc atgtgccacc ggcggcgcac     7440

tcaaaggtta ccaaataaca aacaaattcg agtccttcaa atcgatgagt actcagaatg     7500

ggttcatatt ttatataccg gcgtggctta cgtctaagat cgacccgtca actggttttg     7560

tcaacctgtt gaagacgaaa tacacgtcca ttgccgattc aaaaaagttc atatctagtt     7620

ttgatcgtat tatgtacgtc ccagaggaag atcttttcga gtttgctctc gactacaaaa     7680

acttttcgcg caccgatgcg gattacatta aaaaatggaa actctattcg tacggcaaca     7740

gaatcaggat ttttcgcaac cctaagaaga ataacgtctt tgattgggag gaagtttgct     7800

tgactagcgc gtacaaggag ctctttaata agtatggcat taactaccaa cagggtgata     7860

tcagagcact gctttgcgaa caatctgaca aggctttcta ctcatccttc atggctttga     7920

tgagcctgat gctccagatg agaaattcaa ttacaggcag aaccgacgtg gatttcttga     7980

tctccccggt taaaaattct gatggcatct tttacgatag caggaactat gaagcgcaag     8040

agaatgcgat tctgccaaaa aatgcagacg ccaacggtgc ctataacatc gccaggaaag     8100

tcctgtgggc gatcggccag ttcaaaaagg ccgaagacga aaaattggac aaggtcaaaa     8160

tcgctatcag caacaaagag tggctggagt atgctcagac atccgtaaag cataagcgtc     8220

ctgctgccac caaaaaggcc ggacaggcta agaaaaagaa gtgattaatc ggaccctaga     8280

cttgtccatc ttctggattg gccaacttaa ttaatgtatg aaataaaagg atgcacacat     8340

agtgacatgc taatcactat aatgtgggca tcaaagttgt gtgttatgtg taattactaa     8400

ttatctgaat aagagaaaga gatcatccat atttcttatc ctaaatgaat gtcacgtgtc     8460

tttataattc tttgatgaac cagatgcatt ttattaacca attccatata ccggaccgaa     8520

tctagattca gaacgaatta actagctagt ccaccctcac aaaaaaggtt attgcctaat     8580

taatagtagg gattgaagaa gtatcaataa tcaataagag ggacatttgg attgacacaa     8640

actattagta taaaaaaaaa atgtaaaact aaatggacaa caatctaaga aagcgagtat     8700

gtgttgtact cggaaaacat aacataatgt gattttgatt gtggaaactg aaaacaataa     8760

caattaagtt ttatttatct ctttgcctaa caattttttt aattactttt tttatagttt     8820

ttatgatgaa gataattaat attttttaca aatattatat tttccttttt accatctttg     8880

aagataattg cttttttttc caatttagac aaatatttga gtatcaatga tattcctttt     8940

taatccatta ttgcaattta aaagaccatc aatgattaca ttttcaatca aatgacttta     9000

ttagtcttca ctttacatcg atttaaatca aactaataat tttgtatgga ctaaatctct     9060

gaacattttt atatttacaa catattttaa catttattaa ataattagta tttaatacta     9120

ttagtagaat aatgggagta gcaggaggga ggcactgaga gaatagagat ggcatggaag     9180

taagcaatca agtcaaaatc agagttggcc aaccccaaag gctgtagtag gtaagcatgg     9240

cccattttag tttttacatt catctctcat tttcacctca acggttcaga ttcaatctga     9300

ctccccgatc tcagccgtgg attcaaatgc cacctcaggc acatgcaatt ccaaatggat     9360

gaacctaacc cacaatctaa tcttgttact taggggcttt tccgtcatta aatgacacca     9420

cctaccccct tctccctata aatggcaact caatcccccc cttagaactc gcagcgcttg     9480

atttgaggcc aggcaagccc cactcaacca ccacacctct cctcgttcac gctacccctt     9540

tctgctcttc ttctaccttt caaggtactc ttctttccct ctgttgctgc aaccttctct     9600

ttctttaaga ttgcctcaat ttcggatctt gcacctctgg gttgctttgc tttgcttttt     9660

cctctactgg gttgatttct gtttccctaa accggtttag acgaatgtga acactacttc     9720

ttttgtttaa ttactctgga atacgtgtta ggctttcaga tctagttgaa atcgtattgc     9780

acttttaggg ggagtttgga tttctaataa gaaattgacc ttttgctgag aattggttcg     9840

gtgattagag ggtttccgta aatttttgaa gttttacatg cttgtatctg tttatttttg     9900

tttctcacat ctattattgt taggtgaagg aaattatgta ttgagagtct gtctgatact     9960

aaatataaac acctcaatag gggctctaac actgatttta tcatttgctg cttgtgtgta    10020

tggttaaaga aaggcaattg tgttttaatt ttctgcaagc tttcgtttgc tgaattttat    10080

gcatatattt tcctcccttt tgtgaacttc ctttttgtag ttctaattcc atttttggtg    10140

tctgcagttt taaaagtata aagggatcca aattactgat gagtccgtga ggacgaaacg    10200

agtaagctcg tctaatttct actaagtgta gatccacatc tctttagccc tatcaaggcc    10260

ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg    10320

ggacagcttt ttgtgatctg atgataagtg gttggttcgt gtctcatgca cttgggaggt    10380

gatctatttc acctggtgta gtttgtgttt ccgtcagttg gaaaaactta tccctatcga    10440

tttcgttttc attttctgct tttcttttat gtaccttcgt ttgggcttgt aacgggcctt    10500

tgtatttcaa ctctcaataa taatccaagt gcatgttaaa caatttgtca tctgtttcgg    10560

ctttgatata ctactggtga agatgggccg tactactgca tcacaacgaa aaataataat    10620

aagatgaaaa acttgaagtg gaaaaaaaaa acttgaatgt tcactactac ggacgaccgc    10680

tagtgcatgc actgactaat tagctaactg gcagacaaag tggcagacat actgtcccac    10740

aaatgaagat ggaatctgta aaagaaaacg cgtgaaataa tgcgtctgac aaaggttagg    10800

tcggctgcct ttaatcaata ccaaagtggt ccctaccacg atggaaaaac tgtgcagtcg    10860

gtttggcttt ttctgacgaa caaataagat tcgtggccga caggtggggg tccaccatgt    10920

gaaggcatct tcagactcca ataatggagc aatgacgtaa gggcttacga aataagtaag    10980

ggtagtttgg gaaatgtcca ctcacccgtc agtctataaa tacttagccc ctccctcatt    11040

gttaagggag caagacctaa accatgattg aacaagatgg attgcacgca ggttctccgg    11100

ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg    11160

atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc    11220

tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga    11280

cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc    11340

tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag    11400

tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat    11460

tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg    11520

tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca    11580

ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccacggc gatgcctgct    11640

tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg    11700

gtgtggcaga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg    11760

gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc    11820

gcatcgcctt ctatcgcctt cttgacgagt tcttctgatg agagctctag atccccgaat    11880

ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt    11940

cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg    12000

taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt    12060

taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg    12120

tcatctatgt tactagatca ttagcctgca ggcccgggtt agtctaatta gctaacggcc    12180

aggatcgccg cgtgagcctt tagcaactag ctagagcgat cgccgcaatc tgttattaag    12240

ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct    12300

ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagaattaat    12360

tctcatgttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca    12420

gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct    12480

caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa    12540

atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg    12600

agcggataac aatttcacac aggaaacaga ccatgaggga agcgttgatc gccgaagtat    12660

cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg    12720

ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt    12780

tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc    12840

ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca    12900

ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg    12960

gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg    13020

atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg    13080

cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct    13140

taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt    13200

tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg    13260

actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctaggcagg    13320

cttatcttgg acaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc    13380

actacgtgaa aggcgagatc accaaagtag tcggcaaata aagctctagt ggatctccgt    13440

acccagggat ctggctcgcg gcggacgcac gacgccgggg cgagaccata ggcgatctcc    13500

taaatcaata gtagctgtaa cctcgaagcg tttcacttgt aacaacgatt gagaattttt    13560

gtcataaaat tgaaatactt ggttcgcatt tttgtcatcc gcggtcagcc gcaattctga    13620

cgaactgccc atttagctgg agatgattgt acatccttca cgtgaaaatt tctcaagcgc    13680

tgtgaacaag ggttcagatt ttagattgaa aggtgagccg ttgaaacacg ttcttcttgt    13740

cgatgacgac gtcgctatgc ggcatcttat tattgaatac cttacgatcc acgccttcaa    13800

agtgaccgcg gtagccgaca gcacccagtt cacaagagta ctctcttccg cgacggtcga    13860

tgtcgtggtt gttgatctag atttaggtcg tgaagatggg ctcgagatcg ttcgtaatct    13920

ggcggcaaag tctgatattc caatcataat tatcagtggc gaccgccttg aggagacaga    13980

taaagttgtt gcactcgagc taggagcaag tgattttatc gctaagccgt tcagtatcag    14040

agagtttcta gcacgcattc gggttgcctt gcgcgtgcgc cccaacgttg tccgctccaa    14100

agaccgacgg tctttttgtt ttactgactg gacacttaat ctcaggcaac gtcgcttgat    14160

gtccgaagct ggcggtgagg tgaaacttac ggcaggtgag ttcaatcttc tcctcgcgtt    14220

tttagagaaa ccccgcgacg ttctatcgcg cgagcaactt ctcattgcca gtcgagtacg    14280

cgacgaggag gtttatgaca ggagtataga tgttctcatt ttgaggctgc gccgcaaact    14340

tgaggcagat ccgtcaagcc ctcaactgat aaaaacagca agaggtgccg gttatttctt    14400

tgacgcggac gtgcaggttt cgcacggggg gacgatggca gcctgagcca attcccagat    14460

ccccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg    14520

ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc    14580

gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa    14640

tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag    14700

caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc    14760

atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc    14820

tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg    14880

tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac    14940

cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc    15000

aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt    15060

cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg    15120

gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc    15180

gggcggccgg agtacatcga gatcgagctg gctgattgga tgtaccgcga gatcacagaa    15240

ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc    15300

ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg    15360

ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc    15420

gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg    15480

caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc    15540

ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga    15600

aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac    15660

cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg    15720

actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa    15780

actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg    15840

caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct    15900

atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc    15960

ggacaagccg cgccgtcgcc actcgaccgc cggcgctgag gtctgcctcg tgaagaaggt    16020

gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca    16080

cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc    16140

acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt    16200

cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca    16260

accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat    16320

tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa    16380

actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc    16440

gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga    16500

aatcaccatg agtgacgact gaatccggtg agaatggcaa aagctctgca ttaatgaatc    16560

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    16620

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    16680

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    16740

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    16800

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    16860

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    16920

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    16980

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    17040

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    17100

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg    17160

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga    17220

agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt    17280

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag    17340

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct    17400

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg    17460

atcttcacct agatcctttt gatccggaat ta                                  17492


<210>  5
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4VP16GR

<400>  5

Met Ala Asp Pro Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp 
1               5                   10                  15      


Ile Cys Arg Leu Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys 
            20                  25                  30          


Ala Lys Cys Leu Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr 
        35                  40                  45              


Lys Arg Ser Pro Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg 
    50                  55                  60                  


Leu Glu Arg Leu Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Ser Ser 
65                  70                  75                  80  


Ala Pro Pro Thr Asp Val Ser Leu Gly Asp Glu Leu His Leu Asp Gly 
                85                  90                  95      


Glu Asp Val Ala Met Ala His Ala Asp Ala Leu Asp Asp Phe Asp Leu 
            100                 105                 110         


Asp Met Leu Gly Asp Gly Asp Ser Pro Gly Pro Gly Phe Thr Pro His 
        115                 120                 125             


Asp Ser Ala Pro Tyr Gly Ala Leu Asp Met Ala Asp Phe Glu Phe Glu 
    130                 135                 140                 


Gln Met Phe Thr Asp Ala Leu Gly Ile Asp Glu Tyr Gly Gly Asp Pro 
145                 150                 155                 160 


Ile Gln Gln Ala Thr Ala Gly Val Ser Gln Asp Thr Ser Glu Asn Pro 
                165                 170                 175     


Asn Lys Thr Ile Val Pro Ala Ala Leu Pro Gln Leu Thr Pro Thr Leu 
            180                 185                 190         


Val Ser Leu Leu Glu Val Ile Glu Pro Glu Val Leu Tyr Ala Gly Tyr 
        195                 200                 205             


Asp Ser Ser Val Pro Asp Ser Ala Trp Arg Ile Met Thr Thr Leu Asn 
    210                 215                 220                 


Met Leu Gly Gly Arg Gln Val Ile Ala Ala Val Lys Trp Ala Lys Ala 
225                 230                 235                 240 


Ile Pro Gly Phe Arg Asn Leu His Leu Asp Asp Gln Met Thr Leu Leu 
                245                 250                 255     


Gln Tyr Ser Trp Met Phe Leu Met Ala Phe Ala Leu Gly Trp Arg Ser 
            260                 265                 270         


Tyr Arg Gln Ser Ser Gly Asn Leu Leu Cys Phe Ala Pro Asp Leu Ile 
        275                 280                 285             


Ile Asn Glu Gln Arg Met Ser Leu Pro Cys Met Tyr Asp Gln Cys Lys 
    290                 295                 300                 


His Met Leu Phe Val Ser Ser Glu Leu Gln Arg Leu Gln Val Ser Tyr 
305                 310                 315                 320 


Glu Glu Tyr Leu Cys Met Lys Thr Leu Leu Leu Leu Ser Ser Val Pro 
                325                 330                 335     


Lys Glu Gly Leu Lys Ser Gln Glu Leu Phe Asp Glu Ile Arg Met Thr 
            340                 345                 350         


Tyr Ile Lys Glu Leu Gly Lys Ala Ile Val Lys Arg Glu Gly Asn Ser 
        355                 360                 365             


Ser Gln Asn Trp Gln Arg Phe Tyr Gln Leu Thr Lys Leu Leu Asp Ser 
    370                 375                 380                 


Met His Glu Val Val Glu Asn Leu Leu Thr Tyr Cys Phe Gln Thr Phe 
385                 390                 395                 400 


Leu Asp Lys Thr Met Ser Ile Glu Phe Pro Glu Met Leu Ala Glu Ile 
                405                 410                 415     


Ile Thr Asn Gln Ile Pro Lys Tyr Ser Asn Gly Asn Ile Lys Lys Leu 
            420                 425                 430         


Leu Phe His Gln Lys 
        435         


<210>  6
<211>  277
<212>  PRT
<213>  Rattus norvegicus

<400>  6

Met Gln Gln Ala Thr Ala Gly Val Ser Gln Asp Thr Ser Glu Asn Pro 
1               5                   10                  15      


Asn Lys Thr Ile Val Pro Ala Ala Leu Pro Gln Leu Thr Pro Thr Leu 
            20                  25                  30          


Val Ser Leu Leu Glu Val Ile Glu Pro Glu Val Leu Tyr Ala Gly Tyr 
        35                  40                  45              


Asp Ser Ser Val Pro Asp Ser Ala Trp Arg Ile Met Thr Thr Leu Asn 
    50                  55                  60                  


Met Leu Gly Gly Arg Gln Val Ile Ala Ala Val Lys Trp Ala Lys Ala 
65                  70                  75                  80  


Ile Pro Gly Phe Arg Asn Leu His Leu Asp Asp Gln Met Thr Leu Leu 
                85                  90                  95      


Gln Tyr Ser Trp Met Phe Leu Met Ala Phe Ala Leu Gly Trp Arg Ser 
            100                 105                 110         


Tyr Arg Gln Ser Ser Gly Asn Leu Leu Cys Phe Ala Pro Asp Leu Ile 
        115                 120                 125             


Ile Asn Glu Gln Arg Met Ser Leu Pro Cys Met Tyr Asp Gln Cys Lys 
    130                 135                 140                 


His Met Leu Phe Val Ser Ser Glu Leu Gln Arg Leu Gln Val Ser Tyr 
145                 150                 155                 160 


Glu Glu Tyr Leu Cys Met Lys Thr Leu Leu Leu Leu Ser Ser Val Pro 
                165                 170                 175     


Lys Glu Gly Leu Lys Ser Gln Glu Leu Phe Asp Glu Ile Arg Met Thr 
            180                 185                 190         


Tyr Ile Lys Glu Leu Gly Lys Ala Ile Val Lys Arg Glu Gly Asn Ser 
        195                 200                 205             


Ser Gln Asn Trp Gln Arg Phe Tyr Gln Leu Thr Lys Leu Leu Asp Ser 
    210                 215                 220                 


Met His Glu Val Val Glu Asn Leu Leu Thr Tyr Cys Phe Gln Thr Phe 
225                 230                 235                 240 


Leu Asp Lys Thr Met Ser Ile Glu Phe Pro Glu Met Leu Ala Glu Ile 
                245                 250                 255     


Ile Thr Asn Gln Ile Pro Lys Tyr Ser Asn Gly Asn Ile Lys Lys Leu 
            260                 265                 270         


Leu Phe His Gln Lys 
        275         


<210>  7
<211>  1537
<212>  PRT
<213>  artificial sequence

<220>
<223>  GR-Cas12a

<400>  7

Met Ala Ile Gln Gln Ala Thr Ala Gly Val Ser Gln Asp Thr Ser Glu 
1               5                   10                  15      


Asn Pro Asn Lys Thr Ile Val Pro Ala Ala Leu Pro Gln Leu Thr Pro 
            20                  25                  30          


Thr Leu Val Ser Leu Leu Glu Val Ile Glu Pro Glu Val Leu Tyr Ala 
        35                  40                  45              


Gly Tyr Asp Ser Ser Val Pro Asp Ser Ala Trp Arg Ile Met Thr Thr 
    50                  55                  60                  


Leu Asn Met Leu Gly Gly Arg Gln Val Ile Ala Ala Val Lys Trp Ala 
65                  70                  75                  80  


Lys Ala Ile Pro Gly Phe Arg Asn Leu His Leu Asp Asp Gln Met Thr 
                85                  90                  95      


Leu Leu Gln Tyr Ser Trp Met Phe Leu Met Ala Phe Ala Leu Gly Trp 
            100                 105                 110         


Arg Ser Tyr Arg Gln Ser Ser Gly Asn Leu Leu Cys Phe Ala Pro Asp 
        115                 120                 125             


Leu Ile Ile Asn Glu Gln Arg Met Ser Leu Pro Cys Met Tyr Asp Gln 
    130                 135                 140                 


Cys Lys His Met Leu Phe Val Ser Ser Glu Leu Gln Arg Leu Gln Val 
145                 150                 155                 160 


Ser Tyr Glu Glu Tyr Leu Cys Met Lys Thr Leu Leu Leu Leu Ser Ser 
                165                 170                 175     


Val Pro Lys Glu Gly Leu Lys Ser Gln Glu Leu Phe Asp Glu Ile Arg 
            180                 185                 190         


Met Thr Tyr Ile Lys Glu Leu Gly Lys Ala Ile Val Lys Arg Glu Gly 
        195                 200                 205             


Asn Ser Ser Gln Asn Trp Gln Arg Phe Tyr Gln Leu Thr Lys Leu Leu 
    210                 215                 220                 


Asp Ser Met His Glu Val Val Glu Asn Leu Leu Thr Tyr Cys Phe Gln 
225                 230                 235                 240 


Thr Phe Leu Asp Lys Thr Met Ser Ile Glu Phe Pro Glu Met Leu Ala 
                245                 250                 255     


Glu Ile Ile Thr Asn Gln Ile Pro Lys Tyr Ser Asn Gly Asn Ile Lys 
            260                 265                 270         


Lys Leu Leu Phe His Gln Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        275                 280                 285             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    290                 295                 300                 


Gly Gly Gly Gly Ser Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr 
305                 310                 315                 320 


Ser Leu Ser Lys Thr Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr 
                325                 330                 335     


Gln Glu Asn Ile Asp Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg 
            340                 345                 350         


Ala Glu Asp Tyr Lys Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu 
        355                 360                 365             


Ser Phe Ile Asn Asp Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn 
    370                 375                 380                 


Asn Tyr Ile Ser Leu Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn 
385                 390                 395                 400 


Lys Glu Leu Glu Asn Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys 
                405                 410                 415     


Ala Phe Lys Gly Asn Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile 
            420                 425                 430         


Ile Glu Thr Ile Leu Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala 
        435                 440                 445             


Leu Val Asn Ser Phe Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe 
    450                 455                 460                 


Asp Asn Arg Glu Asn Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile 
465                 470                 475                 480 


Ala Phe Arg Cys Ile Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met 
                485                 490                 495     


Asp Ile Phe Glu Lys Val Asp Ala Ile Phe Asp Lys His Glu Val Gln 
            500                 505                 510         


Glu Ile Lys Glu Lys Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe 
        515                 520                 525             


Phe Glu Gly Glu Phe Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp 
    530                 535                 540                 


Val Tyr Asn Ala Ile Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys 
545                 550                 555                 560 


Ile Lys Gly Leu Asn Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys 
                565                 570                 575     


Gln Lys Leu Pro Lys Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp 
            580                 585                 590         


Arg Glu Ser Leu Ser Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu 
        595                 600                 605             


Val Leu Glu Val Phe Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe 
    610                 615                 620                 


Ser Ser Ile Lys Lys Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr 
625                 630                 635                 640 


Ser Ser Ala Gly Ile Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile 
                645                 650                 655     


Ser Lys Asp Ile Phe Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn 
            660                 665                 670         


Ala Glu Tyr Asp Asp Ile His Leu Lys Lys Lys Ala Val Val Thr Glu 
        675                 680                 685             


Lys Tyr Glu Asp Asp Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe 
    690                 695                 700                 


Ser Leu Glu Gln Leu Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val 
705                 710                 715                 720 


Glu Lys Leu Lys Glu Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys 
                725                 730                 735     


Val Tyr Gly Ser Ser Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu 
            740                 745                 750         


Lys Ser Leu Lys Lys Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu 
        755                 760                 765             


Leu Asp Ser Val Lys Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly 
    770                 775                 780                 


Glu Gly Lys Glu Thr Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val 
785                 790                 795                 800 


Leu Ala Tyr Asp Ile Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile 
                805                 810                 815     


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu 
            820                 825                 830         


Tyr Phe Gln Asn Pro Gln Phe Met Gly Gly Trp Asp Lys Asp Lys Glu 
        835                 840                 845             


Thr Asp Tyr Arg Ala Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu 
    850                 855                 860                 


Ala Ile Met Asp Lys Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys 
865                 870                 875                 880 


Asp Asp Val Asn Gly Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro 
                885                 890                 895     


Gly Pro Asn Lys Met Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met 
            900                 905                 910         


Ala Tyr Tyr Asn Pro Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly 
        915                 920                 925             


Thr Phe Lys Lys Gly Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu 
    930                 935                 940                 


Ile Asp Phe Phe Lys Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn 
945                 950                 955                 960 


Ala Tyr Asp Phe Asn Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala 
                965                 970                 975     


Gly Phe Tyr Arg Glu Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu 
            980                 985                 990         


Ser Ala Ser Lys Lys Glu Val Asp  Lys Leu Val Glu Glu  Gly Lys Leu 
        995                 1000                 1005             


Tyr Met  Phe Gln Ile Tyr Asn  Lys Asp Phe Ser Asp  Lys Ser His 
    1010                 1015                 1020             


Gly Thr  Pro Asn Leu His Thr  Met Tyr Phe Lys Leu  Leu Phe Asp 
    1025                 1030                 1035             


Glu Asn  Asn His Gly Gln Ile  Arg Leu Ser Gly Gly  Ala Glu Leu 
    1040                 1045                 1050             


Phe Met  Arg Arg Ala Ser Leu  Lys Lys Glu Glu Leu  Val Val His 
    1055                 1060                 1065             


Pro Ala  Asn Ser Pro Ile Ala  Asn Lys Asn Pro Asp  Asn Pro Lys 
    1070                 1075                 1080             


Lys Thr  Thr Thr Leu Ser Tyr  Asp Val Tyr Lys Asp  Lys Arg Phe 
    1085                 1090                 1095             


Ser Glu  Asp Gln Tyr Glu Leu  His Ile Pro Ile Ala  Ile Asn Lys 
    1100                 1105                 1110             


Cys Pro  Lys Asn Ile Phe Lys  Ile Asn Thr Glu Val  Arg Val Leu 
    1115                 1120                 1125             


Leu Lys  His Asp Asp Asn Pro  Tyr Val Ile Gly Ile  Asp Arg Gly 
    1130                 1135                 1140             


Glu Arg  Asn Leu Leu Tyr Ile  Val Val Val Asp Gly  Lys Gly Asn 
    1145                 1150                 1155             


Ile Val  Glu Gln Tyr Ser Leu  Asn Glu Ile Ile Asn  Asn Phe Asn 
    1160                 1165                 1170             


Gly Ile  Arg Ile Lys Thr Asp  Tyr His Ser Leu Leu  Asp Lys Lys 
    1175                 1180                 1185             


Glu Lys  Glu Arg Phe Glu Ala  Arg Gln Asn Trp Thr  Ser Ile Glu 
    1190                 1195                 1200             


Asn Ile  Lys Glu Leu Lys Ala  Gly Tyr Ile Ser Gln  Val Val His 
    1205                 1210                 1215             


Lys Ile  Cys Glu Leu Val Glu  Lys Tyr Asp Ala Val  Ile Ala Leu 
    1220                 1225                 1230             


Glu Asp  Leu Asn Ser Gly Phe  Lys Asn Ser Arg Val  Lys Val Glu 
    1235                 1240                 1245             


Lys Gln  Val Tyr Gln Lys Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1250                 1255                 1260             


Asn Tyr  Met Val Asp Lys Lys  Ser Asn Pro Cys Ala  Thr Gly Gly 
    1265                 1270                 1275             


Ala Leu  Lys Gly Tyr Gln Ile  Thr Asn Lys Phe Glu  Ser Phe Lys 
    1280                 1285                 1290             


Ser Met  Ser Thr Gln Asn Gly  Phe Ile Phe Tyr Ile  Pro Ala Trp 
    1295                 1300                 1305             


Leu Thr  Ser Lys Ile Asp Pro  Ser Thr Gly Phe Val  Asn Leu Leu 
    1310                 1315                 1320             


Lys Thr  Lys Tyr Thr Ser Ile  Ala Asp Ser Lys Lys  Phe Ile Ser 
    1325                 1330                 1335             


Ser Phe  Asp Arg Ile Met Tyr  Val Pro Glu Glu Asp  Leu Phe Glu 
    1340                 1345                 1350             


Phe Ala  Leu Asp Tyr Lys Asn  Phe Ser Arg Thr Asp  Ala Asp Tyr 
    1355                 1360                 1365             


Ile Lys  Lys Trp Lys Leu Tyr  Ser Tyr Gly Asn Arg  Ile Arg Ile 
    1370                 1375                 1380             


Phe Arg  Asn Pro Lys Lys Asn  Asn Val Phe Asp Trp  Glu Glu Val 
    1385                 1390                 1395             


Cys Leu  Thr Ser Ala Tyr Lys  Glu Leu Phe Asn Lys  Tyr Gly Ile 
    1400                 1405                 1410             


Asn Tyr  Gln Gln Gly Asp Ile  Arg Ala Leu Leu Cys  Glu Gln Ser 
    1415                 1420                 1425             


Asp Lys  Ala Phe Tyr Ser Ser  Phe Met Ala Leu Met  Ser Leu Met 
    1430                 1435                 1440             


Leu Gln  Met Arg Asn Ser Ile  Thr Gly Arg Thr Asp  Val Asp Phe 
    1445                 1450                 1455             


Leu Ile  Ser Pro Val Lys Asn  Ser Asp Gly Ile Phe  Tyr Asp Ser 
    1460                 1465                 1470             


Arg Asn  Tyr Glu Ala Gln Glu  Asn Ala Ile Leu Pro  Lys Asn Ala 
    1475                 1480                 1485             


Asp Ala  Asn Gly Ala Tyr Asn  Ile Ala Arg Lys Val  Leu Trp Ala 
    1490                 1495                 1500             


Ile Gly  Gln Phe Lys Lys Ala  Glu Asp Glu Lys Leu  Asp Lys Val 
    1505                 1510                 1515             


Lys Ile  Ala Ile Ser Asn Lys  Glu Trp Leu Glu Tyr  Ala Gln Thr 
    1520                 1525                 1530             


Ser Val  Lys His 
    1535         


