                         SEQUENCE LISTING

<110>  HUMANIGEN, INC.
       MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
 
<120>  MATERIALS AND METHODS FOR TREATING CANCER

<130>  P-588784-US2

<140>  17/209,160
<141>  2021-03-22

<150>  PCT/US2019/059275
<151>  2019-10-31

<150>  62/753,485
<151>  2018-10-31

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  1
gacctgccta cagacccgcc                                                   20


<210>  2
<211>  1458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid encoding a chimeric antigen receptor targeting CD19 
       (CAR19)

<400>  2
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcaaac ggggcagaaa gaaactcctg     1020

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     1080

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgagagtgaa gttcagcagg     1140

agcgcagacg cccccgcgta caagcagggc cagaaccagc tctataacga gctcaatcta     1200

ggacgaagag aggagtacga tgttttggac aagagacgtg gccgggaccc tgagatgggg     1260

ggaaagccga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag     1320

atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac     1380

gatggccttt accagggtct cagtacagcc accaaggaca cctacgacgc ccttcacatg     1440

caggccctgc cccctcgc                                                   1458


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  3
tcaggagacg ccgggcctcc                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  4
cagcagcagt gtctctactc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  5
ctcagaaatg tttgacctcc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  6
ggccggtctc actcctggac                                                   20


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a CSF2 gene nucleic acid

<400>  7
gacctgccta cagacccgcc tgg                                               23


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSF2 nucleic acid having a deletion caused by a CRISPR/Cas system

<400>  8
gacctgccta cagaccgcc                                                    19


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSF2 nucleic acid having an insertion caused by a CRISPR/Cas 
       system

<400>  9
gacctgccta cagaccccgc c                                                 21


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a GM-CSF nucleic acid

<400>  10
gcagtgctgc ttgtagtggc                                                   20


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA targeting a CSF2 gene nucleic acid

<400>  11
ccgacctgcc tacagacccg cctgga                                            26


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  12
tgactacaga gaggcacaga                                                   20


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  13
tcacctctga cctcattaac c                                                 21


<210>  14
<211>  54
<212>  DNA
<213>  Homo sapiens

<400>  14
atgtggctgc agagcctgct gctctcgggc ctcgcccagc cccagcacgc agcc             54


<210>  15
<211>  54
<212>  DNA
<213>  Homo sapiens

<400>  15
tacaccgacg tctcggacga cgagagcccg gagcgggtcg gggtcgtgcg tcgg             54


<210>  16
<211>  54
<212>  DNA
<213>  Homo sapiens

<400>  16
aatgaaacag tagaagtcat ctcagaaatg gaagtcatct cagaaatgtt tgac             54


<210>  17
<211>  54
<212>  DNA
<213>  Homo sapiens

<400>  17
ttactttgtc atcttcagta gagtctttac cttcagtaga gtctttacaa actg             54


<210>  18
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  design Exon 3 human GM-CSF

<400>  18
gagccgacct gcctacagac ccgcctggag gcctacagac ccgcctggag ctgta            55


<210>  19
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GM-CSF Exon 3 complementary strand

<400>  19
ctcggctgga cggatgtctg ggcggacctc cggatgtctg ggcggacctc gacat            55


<210>  20
<211>  55
<212>  DNA
<213>  Homo sapiens

<400>  20
gaaacttcct gtgcaaccca gattatcacc tgcaacccag attatcacct ttgaa            55


<210>  21
<211>  55
<212>  DNA
<213>  Homo sapiens

<400>  21
ctttgaagga cacgttgggt ctaatagtgg acgttgggtc taatagtgga aactt            55


<210>  22
<211>  51
<212>  DNA
<213>  Homo sapiens

<400>  22
tggctgcaga gcctgctgct cttgggcact gtggcctgca gcatctctgc a                51


<210>  23
<211>  52
<212>  DNA
<213>  Homo sapiens

<400>  23
ttgggcactg tggcctgcag catctctgca cccgcccgct cgcccagccc ca               52


<210>  24
<211>  50
<212>  DNA
<213>  Homo sapiens

<400>  24
tgtgcaaccc agattatcac ctttgaaagt ttcaaagaga acctgaagga                  50


<210>  25
<211>  50
<212>  DNA
<213>  Homo sapiens

<400>  25
tcctgtgcaa cccagattat cacctttgaa agtttcaaag agaacctgaa                  50


<210>  26
<211>  51
<212>  DNA
<213>  Homo sapiens

<400>  26
ttatcacctt tgaaagtttc aaagagaacc tgaaggactt tctgcttgtc a                51


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA targeting exon 3 of GM-CSF

<400>  27
ccgcccagac atccgtccag                                                   20


<210>  28
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  28
ggctggacgg atgtctgggc ggacct                                            26


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA/Primer CSF2 CRISPR gRNA 1

<400>  29
gacctgccta cagacccgcc                                                   20


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSF2 gRNA1 Deletion

<400>  30
gacctgccta cagaccgcct gg                                                22


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSF2 gRNA1 Insertion

<400>  31
gacctgccta cagaccccgc ctgg                                              24


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSF2 CRISPR gRNA1

<400>  32
ctggacggat gtctgggcgg acc                                               23


