                         SEQUENCE LISTING

<110>  NIPPON MEDICAL SCHOOL FOUNDATION
       MIYAKE, KOICHI
       MATSUMOTO, TAE
 
<120>  GENE THERAPY CONSTRUCTS FOR DELIVERY OF TISSUE-NONSPECIFIC 
       ALKALINE PHOSPHATASE AND METHODS OF USING THE SAME

<130>  4627.002PC01

<150>  US 63/164,928
<151>  2021-03-23

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  4090
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV8-TNALP-D10 Polynucleotide

<400>  1
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

agagcctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg      240

ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg      300

gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg      360

tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta      420

ctgagagtgc accatacctc gagcagtatt gactagttat taatagtaat caattacggg      480

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      540

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      600

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      660

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      720

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      780

gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg      840

cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta      900

attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg      960

gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg     1020

cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg     1080

aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc     1140

cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg     1200

ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt     1260

tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg     1320

ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc     1380

cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga     1440

ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg     1500

ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct     1560

gtaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg     1620

gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg     1680

ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg     1740

cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta     1800

atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg     1860

gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa     1920

ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca     1980

gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt     2040

cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct     2100

tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg     2160

gcaaagaatt cgccaccatg atttcaccat tcttagtact ggccattggc acctgcctta     2220

ctaactcctt agtgccagag aaagagaaag accccaagta ctggcgagac caagcgcaag     2280

agacactgaa atatgccctg gagcttcaga agctcaacac caacgtggct aagaatgtca     2340

tcatgttcct gggagatggg atgggtgtct ccacagtgac ggctgcccgc atcctcaagg     2400

gtcagctcca ccacaaccct ggggaggaga ccaggctgga gatggacaag ttccccttcg     2460

tggccctctc caagacgtac aacaccaatg cccaggtccc tgacagtgcc ggcaccgcca     2520

ccgcctacct gtgtggggtg aaggccaatg agggcaccgt gggggtaagc gcagccactg     2580

agcgttcccg gtgcaacacc acccagggga acgaggtcac ctccatcctg cgctgggcca     2640

aggacgctgg gaaatctgtg ggcattgtga ccaccacgag agtgaaccat gccaccccca     2700

gcgccgccta cgcccactcg gctgaccggg actggtactc agacaacgag atgccccctg     2760

aggccttgag ccagggctgt aaggacatcg cctaccagct catgcataac atcagggaca     2820

ttgacgtgat catggggggt ggccggaaat acatgtaccc caagaataaa actgatgtgg     2880

agtatgagag tgacgagaaa gccaggggca cgaggctgga cggcctggac ctcgttgaca     2940

cctggaagag cttcaaaccg agacacaagc actcccactt catctggaac cgcacggaac     3000

tcctgaccct tgacccccac aatgtggact acctattggg tctcttcgag ccgggggaca     3060

tgcagtacga gctgaacagg aacaacgtga cggacccgtc actctccgag atggtggtgg     3120

tggccatcca gatcctgcgg aagaacccca aaggcttctt cttgctggtg gaaggaggca     3180

gaattgacca cgggcaccat gaaggaaaag ccaagcaggc cctgcatgag gcggtggaga     3240

tggaccgggc catcgggcag gcaggcagct tgacctcctc ggaagacact ctgaccgtgg     3300

tcactgcgga ccattcccac gtcttcacat ttggtggata caccccccgt ggcaactcta     3360

tctttggtct ggcccccatg ctgagtgaca cagacaagaa gcccttcact gccatcctgt     3420

atggcaatgg gcctggctac aaggtggtgg gcggtgaacg agagaatgtc tccatggtgg     3480

actatgctca caacaactac caggcgcagt ctgctgtgcc cctgcgccac gagacccacg     3540

gcggggagga cgtggccgtc ttctccaagg gccccatggc gcacctgctg cacggcgtcc     3600

acgagcagaa ctacgtcccc cacgtgatgg cgtatgcagc ctgcatcggg gccaacctcg     3660

gccactgtgc tcctgccagc tccgcaggca gcgacgatga cgacgatgat gatgatgacg     3720

actgagcggc cgcatgcata agcttggctg caggtcgacg gatccccaac ttgtttattg     3780

cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     3840

tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga     3900

tcgatcctct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca     3960

aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg     4020

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc     4080

gagcgcgcag                                                            4090


<210>  2
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TNALP

<400>  2
atgatttcac cattcttagt actggccatt ggcacctgcc ttactaactc cttagtgcca       60

gagaaagaga aagaccccaa gtactggcga gaccaagcgc aagagacact gaaatatgcc      120

ctggagcttc agaagctcaa caccaacgtg gctaagaatg tcatcatgtt cctgggagat      180

gggatgggtg tctccacagt gacggctgcc cgcatcctca agggtcagct ccaccacaac      240

cctggggagg agaccaggct ggagatggac aagttcccct tcgtggccct ctccaagacg      300

tacaacacca atgcccaggt ccctgacagt gccggcaccg ccaccgccta cctgtgtggg      360

gtgaaggcca atgagggcac cgtgggggta agcgcagcca ctgagcgttc ccggtgcaac      420

accacccagg ggaacgaggt cacctccatc ctgcgctggg ccaaggacgc tgggaaatct      480

gtgggcattg tgaccaccac gagagtgaac catgccaccc ccagcgccgc ctacgcccac      540

tcggctgacc gggactggta ctcagacaac gagatgcccc ctgaggcctt gagccagggc      600

tgtaaggaca tcgcctacca gctcatgcat aacatcaggg acattgacgt gatcatgggg      660

ggtggccgga aatacatgta ccccaagaat aaaactgatg tggagtatga gagtgacgag      720

aaagccaggg gcacgaggct ggacggcctg gacctcgttg acacctggaa gagcttcaaa      780

ccgagacaca agcactccca cttcatctgg aaccgcacgg aactcctgac ccttgacccc      840

cacaatgtgg actacctatt gggtctcttc gagccggggg acatgcagta cgagctgaac      900

aggaacaacg tgacggaccc gtcactctcc gagatggtgg tggtggccat ccagatcctg      960

cggaagaacc ccaaaggctt cttcttgctg gtggaaggag gcagaattga ccacgggcac     1020

catgaaggaa aagccaagca ggccctgcat gaggcggtgg agatggaccg ggccatcggg     1080

caggcaggca gcttgacctc ctcggaagac actctgaccg tggtcactgc ggaccattcc     1140

cacgtcttca catttggtgg atacaccccc cgtggcaact ctatctttgg tctggccccc     1200

atgctgagtg acacagacaa gaagcccttc actgccatcc tgtatggcaa tgggcctggc     1260

tacaaggtgg tgggcggtga acgagagaat gtctccatgg tggactatgc tcacaacaac     1320

taccaggcgc agtctgctgt gcccctgcgc cacgagaccc acggcgggga ggacgtggcc     1380

gtcttctcca agggccccat ggcgcacctg ctgcacggcg tccacgagca gaactacgtc     1440

ccccacgtga tggcgtatgc agcctgcatc ggggccaacc tcggccactg tgctcctgcc     1500

agctccgcag gcagc                                                      1515


<210>  3
<211>  1618
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV Enhancer-CAG promoter

<400>  3
attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga       60

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      120

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      180

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca      240

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc      300

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc      360

tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc      420

cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg      480

gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag      540

gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc      600

gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc      660

gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg      720

actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa      780

ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg      840

gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg      900

cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg      960

cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc     1020

gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg     1080

gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg     1140

agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct     1200

cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg     1260

ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg     1320

cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc     1380

tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg     1440

gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc     1500

gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg     1560

ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggc       1618


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D10

<400>  4
gacgatgacg acgatgatga tgatgacgac                                        30


<210>  5
<211>  1545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TNALP-D10

<400>  5
atgatttcac cattcttagt actggccatt ggcacctgcc ttactaactc cttagtgcca       60

gagaaagaga aagaccccaa gtactggcga gaccaagcgc aagagacact gaaatatgcc      120

ctggagcttc agaagctcaa caccaacgtg gctaagaatg tcatcatgtt cctgggagat      180

gggatgggtg tctccacagt gacggctgcc cgcatcctca agggtcagct ccaccacaac      240

cctggggagg agaccaggct ggagatggac aagttcccct tcgtggccct ctccaagacg      300

tacaacacca atgcccaggt ccctgacagt gccggcaccg ccaccgccta cctgtgtggg      360

gtgaaggcca atgagggcac cgtgggggta agcgcagcca ctgagcgttc ccggtgcaac      420

accacccagg ggaacgaggt cacctccatc ctgcgctggg ccaaggacgc tgggaaatct      480

gtgggcattg tgaccaccac gagagtgaac catgccaccc ccagcgccgc ctacgcccac      540

tcggctgacc gggactggta ctcagacaac gagatgcccc ctgaggcctt gagccagggc      600

tgtaaggaca tcgcctacca gctcatgcat aacatcaggg acattgacgt gatcatgggg      660

ggtggccgga aatacatgta ccccaagaat aaaactgatg tggagtatga gagtgacgag      720

aaagccaggg gcacgaggct ggacggcctg gacctcgttg acacctggaa gagcttcaaa      780

ccgagacaca agcactccca cttcatctgg aaccgcacgg aactcctgac ccttgacccc      840

cacaatgtgg actacctatt gggtctcttc gagccggggg acatgcagta cgagctgaac      900

aggaacaacg tgacggaccc gtcactctcc gagatggtgg tggtggccat ccagatcctg      960

cggaagaacc ccaaaggctt cttcttgctg gtggaaggag gcagaattga ccacgggcac     1020

catgaaggaa aagccaagca ggccctgcat gaggcggtgg agatggaccg ggccatcggg     1080

caggcaggca gcttgacctc ctcggaagac actctgaccg tggtcactgc ggaccattcc     1140

cacgtcttca catttggtgg atacaccccc cgtggcaact ctatctttgg tctggccccc     1200

atgctgagtg acacagacaa gaagcccttc actgccatcc tgtatggcaa tgggcctggc     1260

tacaaggtgg tgggcggtga acgagagaat gtctccatgg tggactatgc tcacaacaac     1320

taccaggcgc agtctgctgt gcccctgcgc cacgagaccc acggcgggga ggacgtggcc     1380

gtcttctcca agggccccat ggcgcacctg ctgcacggcg tccacgagca gaactacgtc     1440

ccccacgtga tggcgtatgc agcctgcatc ggggccaacc tcggccactg tgctcctgcc     1500

agctccgcag gcagcgacga tgacgacgat gatgatgatg acgac                     1545


<210>  6
<211>  3275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV enhancer-CAG Promoter-TNALP-D10

<400>  6
attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga       60

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      120

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      180

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca      240

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc      300

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc      360

tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc      420

cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg      480

gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag      540

gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc      600

gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc      660

gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg      720

actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa      780

ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg      840

gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg      900

cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg      960

cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc     1020

gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg     1080

gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg     1140

agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct     1200

cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg     1260

ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg     1320

cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc     1380

tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg     1440

gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc     1500

gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg     1560

ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc     1620

tagctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca     1680

acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc atgatttcac     1740

cattcttagt actggccatt ggcacctgcc ttactaactc cttagtgcca gagaaagaga     1800

aagaccccaa gtactggcga gaccaagcgc aagagacact gaaatatgcc ctggagcttc     1860

agaagctcaa caccaacgtg gctaagaatg tcatcatgtt cctgggagat gggatgggtg     1920

tctccacagt gacggctgcc cgcatcctca agggtcagct ccaccacaac cctggggagg     1980

agaccaggct ggagatggac aagttcccct tcgtggccct ctccaagacg tacaacacca     2040

atgcccaggt ccctgacagt gccggcaccg ccaccgccta cctgtgtggg gtgaaggcca     2100

atgagggcac cgtgggggta agcgcagcca ctgagcgttc ccggtgcaac accacccagg     2160

ggaacgaggt cacctccatc ctgcgctggg ccaaggacgc tgggaaatct gtgggcattg     2220

tgaccaccac gagagtgaac catgccaccc ccagcgccgc ctacgcccac tcggctgacc     2280

gggactggta ctcagacaac gagatgcccc ctgaggcctt gagccagggc tgtaaggaca     2340

tcgcctacca gctcatgcat aacatcaggg acattgacgt gatcatgggg ggtggccgga     2400

aatacatgta ccccaagaat aaaactgatg tggagtatga gagtgacgag aaagccaggg     2460

gcacgaggct ggacggcctg gacctcgttg acacctggaa gagcttcaaa ccgagacaca     2520

agcactccca cttcatctgg aaccgcacgg aactcctgac ccttgacccc cacaatgtgg     2580

actacctatt gggtctcttc gagccggggg acatgcagta cgagctgaac aggaacaacg     2640

tgacggaccc gtcactctcc gagatggtgg tggtggccat ccagatcctg cggaagaacc     2700

ccaaaggctt cttcttgctg gtggaaggag gcagaattga ccacgggcac catgaaggaa     2760

aagccaagca ggccctgcat gaggcggtgg agatggaccg ggccatcggg caggcaggca     2820

gcttgacctc ctcggaagac actctgaccg tggtcactgc ggaccattcc cacgtcttca     2880

catttggtgg atacaccccc cgtggcaact ctatctttgg tctggccccc atgctgagtg     2940

acacagacaa gaagcccttc actgccatcc tgtatggcaa tgggcctggc tacaaggtgg     3000

tgggcggtga acgagagaat gtctccatgg tggactatgc tcacaacaac taccaggcgc     3060

agtctgctgt gcccctgcgc cacgagaccc acggcgggga ggacgtggcc gtcttctcca     3120

agggccccat ggcgcacctg ctgcacggcg tccacgagca gaactacgtc ccccacgtga     3180

tggcgtatgc agcctgcatc ggggccaacc tcggccactg tgctcctgcc agctccgcag     3240

gcagcgacga tgacgacgat gatgatgatg acgac                                3275


<210>  7
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNALP Amino Acid Sequence

<400>  7

Met Ile Ser Pro Phe Leu Val Leu Ala Ile Gly Thr Cys Leu Thr Asn 
1               5                   10                  15      


Ser Leu Val Pro Glu Lys Glu Lys Asp Pro Lys Tyr Trp Arg Asp Gln 
            20                  25                  30          


Ala Gln Glu Thr Leu Lys Tyr Ala Leu Glu Leu Gln Lys Leu Asn Thr 
        35                  40                  45              


Asn Val Ala Lys Asn Val Ile Met Phe Leu Gly Asp Gly Met Gly Val 
    50                  55                  60                  


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Leu His His Asn 
65                  70                  75                  80  


Pro Gly Glu Glu Thr Arg Leu Glu Met Asp Lys Phe Pro Phe Val Ala 
                85                  90                  95      


Leu Ser Lys Thr Tyr Asn Thr Asn Ala Gln Val Pro Asp Ser Ala Gly 
            100                 105                 110         


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Ala Asn Glu Gly Thr Val 
        115                 120                 125             


Gly Val Ser Ala Ala Thr Glu Arg Ser Arg Cys Asn Thr Thr Gln Gly 
    130                 135                 140                 


Asn Glu Val Thr Ser Ile Leu Arg Trp Ala Lys Asp Ala Gly Lys Ser 
145                 150                 155                 160 


Val Gly Ile Val Thr Thr Thr Arg Val Asn His Ala Thr Pro Ser Ala 
                165                 170                 175     


Ala Tyr Ala His Ser Ala Asp Arg Asp Trp Tyr Ser Asp Asn Glu Met 
            180                 185                 190         


Pro Pro Glu Ala Leu Ser Gln Gly Cys Lys Asp Ile Ala Tyr Gln Leu 
        195                 200                 205             


Met His Asn Ile Arg Asp Ile Asp Val Ile Met Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Tyr Pro Lys Asn Lys Thr Asp Val Glu Tyr Glu Ser Asp Glu 
225                 230                 235                 240 


Lys Ala Arg Gly Thr Arg Leu Asp Gly Leu Asp Leu Val Asp Thr Trp 
                245                 250                 255     


Lys Ser Phe Lys Pro Arg His Lys His Ser His Phe Ile Trp Asn Arg 
            260                 265                 270         


Thr Glu Leu Leu Thr Leu Asp Pro His Asn Val Asp Tyr Leu Leu Gly 
        275                 280                 285             


Leu Phe Glu Pro Gly Asp Met Gln Tyr Glu Leu Asn Arg Asn Asn Val 
    290                 295                 300                 


Thr Asp Pro Ser Leu Ser Glu Met Val Val Val Ala Ile Gln Ile Leu 
305                 310                 315                 320 


Arg Lys Asn Pro Lys Gly Phe Phe Leu Leu Val Glu Gly Gly Arg Ile 
                325                 330                 335     


Asp His Gly His His Glu Gly Lys Ala Lys Gln Ala Leu His Glu Ala 
            340                 345                 350         


Val Glu Met Asp Arg Ala Ile Gly Gln Ala Gly Ser Leu Thr Ser Ser 
        355                 360                 365             


Glu Asp Thr Leu Thr Val Val Thr Ala Asp His Ser His Val Phe Thr 
    370                 375                 380                 


Phe Gly Gly Tyr Thr Pro Arg Gly Asn Ser Ile Phe Gly Leu Ala Pro 
385                 390                 395                 400 


Met Leu Ser Asp Thr Asp Lys Lys Pro Phe Thr Ala Ile Leu Tyr Gly 
                405                 410                 415     


Asn Gly Pro Gly Tyr Lys Val Val Gly Gly Glu Arg Glu Asn Val Ser 
            420                 425                 430         


Met Val Asp Tyr Ala His Asn Asn Tyr Gln Ala Gln Ser Ala Val Pro 
        435                 440                 445             


Leu Arg His Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ser Lys 
    450                 455                 460                 


Gly Pro Met Ala His Leu Leu His Gly Val His Glu Gln Asn Tyr Val 
465                 470                 475                 480 


Pro His Val Met Ala Tyr Ala Ala Cys Ile Gly Ala Asn Leu Gly His 
                485                 490                 495     


Cys Ala Pro Ala Ser Ser 
            500         


<210>  8
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D10 Amino Acid Sequence

<400>  8

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10  


<210>  9
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNALP-D10 Amino Acid Sequence

<400>  9

Met Ile Ser Pro Phe Leu Val Leu Ala Ile Gly Thr Cys Leu Thr Asn 
1               5                   10                  15      


Ser Leu Val Pro Glu Lys Glu Lys Asp Pro Lys Tyr Trp Arg Asp Gln 
            20                  25                  30          


Ala Gln Glu Thr Leu Lys Tyr Ala Leu Glu Leu Gln Lys Leu Asn Thr 
        35                  40                  45              


Asn Val Ala Lys Asn Val Ile Met Phe Leu Gly Asp Gly Met Gly Val 
    50                  55                  60                  


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Leu His His Asn 
65                  70                  75                  80  


Pro Gly Glu Glu Thr Arg Leu Glu Met Asp Lys Phe Pro Phe Val Ala 
                85                  90                  95      


Leu Ser Lys Thr Tyr Asn Thr Asn Ala Gln Val Pro Asp Ser Ala Gly 
            100                 105                 110         


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Ala Asn Glu Gly Thr Val 
        115                 120                 125             


Gly Val Ser Ala Ala Thr Glu Arg Ser Arg Cys Asn Thr Thr Gln Gly 
    130                 135                 140                 


Asn Glu Val Thr Ser Ile Leu Arg Trp Ala Lys Asp Ala Gly Lys Ser 
145                 150                 155                 160 


Val Gly Ile Val Thr Thr Thr Arg Val Asn His Ala Thr Pro Ser Ala 
                165                 170                 175     


Ala Tyr Ala His Ser Ala Asp Arg Asp Trp Tyr Ser Asp Asn Glu Met 
            180                 185                 190         


Pro Pro Glu Ala Leu Ser Gln Gly Cys Lys Asp Ile Ala Tyr Gln Leu 
        195                 200                 205             


Met His Asn Ile Arg Asp Ile Asp Val Ile Met Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Tyr Pro Lys Asn Lys Thr Asp Val Glu Tyr Glu Ser Asp Glu 
225                 230                 235                 240 


Lys Ala Arg Gly Thr Arg Leu Asp Gly Leu Asp Leu Val Asp Thr Trp 
                245                 250                 255     


Lys Ser Phe Lys Pro Arg His Lys His Ser His Phe Ile Trp Asn Arg 
            260                 265                 270         


Thr Glu Leu Leu Thr Leu Asp Pro His Asn Val Asp Tyr Leu Leu Gly 
        275                 280                 285             


Leu Phe Glu Pro Gly Asp Met Gln Tyr Glu Leu Asn Arg Asn Asn Val 
    290                 295                 300                 


Thr Asp Pro Ser Leu Ser Glu Met Val Val Val Ala Ile Gln Ile Leu 
305                 310                 315                 320 


Arg Lys Asn Pro Lys Gly Phe Phe Leu Leu Val Glu Gly Gly Arg Ile 
                325                 330                 335     


Asp His Gly His His Glu Gly Lys Ala Lys Gln Ala Leu His Glu Ala 
            340                 345                 350         


Val Glu Met Asp Arg Ala Ile Gly Gln Ala Gly Ser Leu Thr Ser Ser 
        355                 360                 365             


Glu Asp Thr Leu Thr Val Val Thr Ala Asp His Ser His Val Phe Thr 
    370                 375                 380                 


Phe Gly Gly Tyr Thr Pro Arg Gly Asn Ser Ile Phe Gly Leu Ala Pro 
385                 390                 395                 400 


Met Leu Ser Asp Thr Asp Lys Lys Pro Phe Thr Ala Ile Leu Tyr Gly 
                405                 410                 415     


Asn Gly Pro Gly Tyr Lys Val Val Gly Gly Glu Arg Glu Asn Val Ser 
            420                 425                 430         


Met Val Asp Tyr Ala His Asn Asn Tyr Gln Ala Gln Ser Ala Val Pro 
        435                 440                 445             


Leu Arg His Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ser Lys 
    450                 455                 460                 


Gly Pro Met Ala His Leu Leu His Gly Val His Glu Gln Asn Tyr Val 
465                 470                 475                 480 


Pro His Val Met Ala Tyr Ala Ala Cys Ile Gly Ala Asn Leu Gly His 
                485                 490                 495     


Cys Ala Pro Ala Ser Ser Ala Gly Ser Asp Asp Asp Asp Asp Asp Asp 
            500                 505                 510         


Asp Asp Asp 
        515 


<210>  10
<211>  7681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV8-TNALP-D10 Vector Sequence

<400>  10
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

agagcctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg      240

ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg      300

gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg      360

tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta      420

ctgagagtgc accatacctc gagcagtatt gactagttat taatagtaat caattacggg      480

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      540

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      600

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      660

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      720

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      780

gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg      840

cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta      900

attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg      960

gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg     1020

cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg     1080

aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc     1140

cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg     1200

ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt     1260

tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg     1320

ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc     1380

cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga     1440

ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg     1500

ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct     1560

gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg     1620

gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg     1680

ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg     1740

cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta     1800

atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg     1860

gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa     1920

ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca     1980

gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt     2040

cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct     2100

tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg     2160

gcaaagaatt cgccaccatg atttcaccat tcttagtact ggccattggc acctgcctta     2220

ctaactcctt agtgccagag aaagagaaag accccaagta ctggcgagac caagcgcaag     2280

agacactgaa atatgccctg gagcttcaga agctcaacac caacgtggct aagaatgtca     2340

tcatgttcct gggagatggg atgggtgtct ccacagtgac ggctgcccgc atcctcaagg     2400

gtcagctcca ccacaaccct ggggaggaga ccaggctgga gatggacaag ttccccttcg     2460

tggccctctc caagacgtac aacaccaatg cccaggtccc tgacagtgcc ggcaccgcca     2520

ccgcctacct gtgtggggtg aaggccaatg agggcaccgt gggggtaagc gcagccactg     2580

agcgttcccg gtgcaacacc acccagggga acgaggtcac ctccatcctg cgctgggcca     2640

aggacgctgg gaaatctgtg ggcattgtga ccaccacgag agtgaaccat gccaccccca     2700

gcgccgccta cgcccactcg gctgaccggg actggtactc agacaacgag atgccccctg     2760

aggccttgag ccagggctgt aaggacatcg cctaccagct catgcataac atcagggaca     2820

ttgacgtgat catggggggt ggccggaaat acatgtaccc caagaataaa actgatgtgg     2880

agtatgagag tgacgagaaa gccaggggca cgaggctgga cggcctggac ctcgttgaca     2940

cctggaagag cttcaaaccg agacacaagc actcccactt catctggaac cgcacggaac     3000

tcctgaccct tgacccccac aatgtggact acctattggg tctcttcgag ccgggggaca     3060

tgcagtacga gctgaacagg aacaacgtga cggacccgtc actctccgag atggtggtgg     3120

tggccatcca gatcctgcgg aagaacccca aaggcttctt cttgctggtg gaaggaggca     3180

gaattgacca cgggcaccat gaaggaaaag ccaagcaggc cctgcatgag gcggtggaga     3240

tggaccgggc catcgggcag gcaggcagct tgacctcctc ggaagacact ctgaccgtgg     3300

tcactgcgga ccattcccac gtcttcacat ttggtggata caccccccgt ggcaactcta     3360

tctttggtct ggcccccatg ctgagtgaca cagacaagaa gcccttcact gccatcctgt     3420

atggcaatgg gcctggctac aaggtggtgg gcggtgaacg agagaatgtc tccatggtgg     3480

actatgctca caacaactac caggcgcagt ctgctgtgcc cctgcgccac gagacccacg     3540

gcggggagga cgtggccgtc ttctccaagg gccccatggc gcacctgctg cacggcgtcc     3600

acgagcagaa ctacgtcccc cacgtgatgg cgtatgcagc ctgcatcggg gccaacctcg     3660

gccactgtgc tcctgccagc tccgcaggca gcgacgatga cgacgatgat gatgatgacg     3720

actgagcggc cgcatgcata agcttggctg caggtcgacg gatccccaac ttgtttattg     3780

cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     3840

tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga     3900

tcgatcctct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca     3960

aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg     4020

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc     4080

gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     4140

gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat     4200

attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat     4260

caaagaagta ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc     4320

ctcactgatt ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct     4380

ttaatcggcc tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg     4440

ctcgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt     4500

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt     4560

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct     4620

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg     4680

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga     4740

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc     4800

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga     4860

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaaat     4920

atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat     4980

gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc     5040

aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg     5100

aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt     5160

tctcacccgt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag     5220

ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag     5280

ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat     5340

tttgctaatt ctttgccttg cctgtatgat ttattggatg ttggaatcgc ctgatgcggt     5400

attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa     5460

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc     5520

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     5580

gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg     5640

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg     5700

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa     5760

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     5820

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     5880

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     5940

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     6000

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     6060

tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     6120

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     6180

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     6240

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     6300

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     6360

cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     6420

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     6480

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg     6540

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta     6600

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     6660

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga     6720

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     6780

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     6840

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     6900

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     6960

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt     7020

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     7080

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     7140

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     7200

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     7260

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     7320

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     7380

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     7440

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc     7500

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt     7560

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag     7620

cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca     7680

g                                                                     7681


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
agtccgtggg cattgtgact a                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
tgctgctcca ctcacgtcga t                                                 21


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D8

<400>  13
gacgatgacg acgatgatga tgat                                              24


<210>  14
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D9

<400>  14
gacgatgacg acgatgatga tgatgac                                           27


<210>  15
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D11

<400>  15
gacgatgacg acgatgatga tgatgacgac gac                                    33


<210>  16
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D12

<400>  16
gacgatgacg acgatgatga tgatgacgac gacgat                                 36


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D8 Amino Acid Sequence

<400>  17

Asp Asp Asp Asp Asp Asp Asp Asp 
1               5               


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D9 Amino Acid Sequence

<400>  18

Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   


<210>  19
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D11 Amino Acid Sequence

<400>  19

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10      


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D12 Amino Acid Sequence

<400>  20

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10          


<210>  21
<211>  1242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAG Promoter

<400>  21
gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg       60

tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc      120

gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg      180

gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg      240

cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc      300

gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc      360

ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa      420

tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct      480

ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg      540

tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg      600

ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc      660

tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg      720

ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc      780

ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg      840

gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg      900

ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc      960

cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt     1020

gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg     1080

tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt     1140

ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga     1200

cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gc                        1242


<210>  22
<211>  358
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV Enhancer

<400>  22
agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc       60

gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg      120

acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa      180

tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca      240

agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac      300

atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctatta        358


