                        б
<110>  Ƽ޹˾ 
<120>  AAV鵼֬֬øλ弰;
<130>  
<160>  14    
<170>  PatentIn汾3.3
<210>  1
<211>  1425
<212>  DNA
<213>  ˹
<220>
<223>  LPL-S447Xĺ
<400>  1
atggagtcca aggccctgct ggtgctgaca ctggccgtgt ggctgcagtc tctgaccgca     60
agcaggggag gagtggcagc agcagaccag cggagagact tcatcgatat cgagtccaag    120
tttgcactga ggaccccaga ggacacagca gaggatacct gccacctgat ccctggcgtg    180
gccgagtctg tggccacatg tcacttcaac cacagctcca agaccttcat ggtcatccac    240
ggctggaccg tgacaggcat gtacgagagc tgggtgccta agctggtggc cgccctgtat    300
aagagggagc cagactctaa cgtgatcgtg gtggattggc tgagcagggc acaggagcac    360
tacccagtgt ccgccggcta taccaagctg gtgggacagg acgtggcacg gttcatcaat    420
tggatggagg aggagtttaa ctaccccctg gataatgtgc acctgctggg atattctctg    480
ggagcacacg cagcaggaat cgcaggaagc ctgacaaaca agaaagtgaa taggatcacc    540
ggactggatc cagcaggacc taatttcgag tacgcagagg cacctagcag gctgtcccca    600
gacgatgccg acttcgtgga tgtgctgcac acctttacaa ggggatcccc aggccgctct    660
atcggaatcc agaagcccgt gggccacgtg gacatctatc ccaacggcgg cacctttcag    720
cctggctgca atatcggcga ggccatcaga gtgatcgccg agagaggcct gggcgacgtg    780
gatcagctgg tgaagtgtag ccacgagcgg tccatccacc tgttcatcga ttccctgctg    840
aacgaggaga atccctctaa ggcctacaga tgctctagca aggaggcctt tgagaagggc    900
ctgtgcctgt cctgtaggaa gaaccgctgt aacaatctgg gctacgagat caataaggtg    960
cgggccaaga gatcctctaa gatgtatctg aagacacgga gccagatgcc ttacaaggtg   1020
ttccactatc aggtgaagat ccacttttct ggcaccgaga gcgagaccca cacaaaccag   1080
gccttcgaga tcagcctgta cggcacagtg gccgagtccg agaacatccc attcaccctg   1140
cccgaggtgt ctaccaataa gacatacagc tttctgatct atacagaggt ggacatcggc   1200
gagctgctga tgctgaagct gaagtggaag tctgacagct atttctcctg gtctgattgg   1260
tggagctccc ctggctttgc catccagaag atccgggtga aggccggcga gacccagaag   1320
aaagtgatct tctgctccag agagaaggtg tctcacctgc agaagggcaa ggccccagcc   1380
gtgtttgtga agtgtcacga taagagcctg aataagaagt gataa                   1425
<210>  2
<211>  82
<212>  DNA
<213>  ˹
<220>
<223>  14Ⱦɫ򵰰ں
<400>  2
gtgagtatct cagggatcca gacatgggga tatgggaggt gcctctgatc ccagggctca     60
ctgtgggtct ctctgttcac ag                                              82
<210>  3
<211>  1507
<212>  DNA
<213>  ˹
<220>
<223>  SEQ ID NO:2ʾںӵıLPL-S447Xĺ
<400>  3
atggagtcca aggccctgct ggtgctgaca ctggccgtgt ggctgcagtc tctgaccgca     60
agcaggggag gagtggcagc agcagaccag cggagagact tcatcgatat cgagtccaag    120
tttgcactga ggaccccaga ggacacagca gaggatacct gccacctgat ccctggcgtg    180
gccgagtctg tggccacatg tcacttcaac cacagctcca agaccttcat ggtcatccac    240
ggctggaccg tgacaggtga gtatctcagg gatccagaca tggggatatg ggaggtgcct    300
ctgatcccag ggctcactgt gggtctctct gttcacaggc atgtacgaga gctgggtgcc    360
taagctggtg gccgccctgt ataagaggga gccagactct aacgtgatcg tggtggattg    420
gctgagcagg gcacaggagc actacccagt gtccgccggc tataccaagc tggtgggaca    480
ggacgtggca cggttcatca attggatgga ggaggagttt aactaccccc tggataatgt    540
gcacctgctg ggatattctc tgggagcaca cgcagcagga atcgcaggaa gcctgacaaa    600
caagaaagtg aataggatca ccggactgga tccagcagga cctaatttcg agtacgcaga    660
ggcacctagc aggctgtccc cagacgatgc cgacttcgtg gatgtgctgc acacctttac    720
aaggggatcc ccaggccgct ctatcggaat ccagaagccc gtgggccacg tggacatcta    780
tcccaacggc ggcacctttc agcctggctg caatatcggc gaggccatca gagtgatcgc    840
cgagagaggc ctgggcgacg tggatcagct ggtgaagtgt agccacgagc ggtccatcca    900
cctgttcatc gattccctgc tgaacgagga gaatccctct aaggcctaca gatgctctag    960
caaggaggcc tttgagaagg gcctgtgcct gtcctgtagg aagaaccgct gtaacaatct   1020
gggctacgag atcaataagg tgcgggccaa gagatcctct aagatgtatc tgaagacacg   1080
gagccagatg ccttacaagg tgttccacta tcaggtgaag atccactttt ctggcaccga   1140
gagcgagacc cacacaaacc aggccttcga gatcagcctg tacggcacag tggccgagtc   1200
cgagaacatc ccattcaccc tgcccgaggt gtctaccaat aagacataca gctttctgat   1260
ctatacagag gtggacatcg gcgagctgct gatgctgaag ctgaagtgga agtctgacag   1320
ctatttctcc tggtctgatt ggtggagctc ccctggcttt gccatccaga agatccgggt   1380
gaaggccggc gagacccaga agaaagtgat cttctgctcc agagagaagg tgtctcacct   1440
gcagaagggc aaggccccag ccgtgtttgt gaagtgtcac gataagagcc tgaataagaa   1500
gtgataa                                                             1507
<210>  4
<211>  6151
<212>  DNA
<213>  ˹
<220>
<223>  pscAAV-LP15-EGFP-kan-4142Tĺ
<400>  4
aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt     60
ctagcttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg    120
tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg    180
gagtggccaa ctccatcact aggggttcct ctcgaggtta atttttaaaa agcaagtggc    240
ccttggcagc atctgtttgc tctggttaat aatctcagga gcacaaacat tcccaggaga    300
agaaatcaac atcctggact tatcctctgg gcctaagtat ttagtttggt tagtaattac    360
taaacactga gaacgccaat gaaatacaaa gatgagtcta gttaataatc tacaattatt    420
ggttaaagaa gtatattagt gctaatttcc ctccgtttgt cctagctttt ctcttctgtc    480
aaccccacac gcctttggca ggtaagttgg cgccgtttaa gggatggttg gttggtgggg    540
tattaatgtt taattacctt ttttacaggg taccgccacc atggtgagca agggcgagga    600
gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa    660
gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt    720
catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta    780
cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc    840
cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaagggcg acggcaacta    900
caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa    960
gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa   1020
cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa   1080
gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac   1140
ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc   1200
cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc   1260
cgccgggatc actctcggca tggacgagct gtacaagtaa agtggccgcg actctagagg   1320
gaattctcca taaagtagga aacactacga tctccataaa gtaggaaaca ctacagtatc   1380
tccataaagt aggaaacact acgctatcca taaagtagga aacactacag atctgcctcg   1440
actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc   1500
ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt   1560
ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat   1620
tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgggtct ggcagccaaa   1680
gcaatcactc aaaggatccc cgccccactc cctctctgcg cgctcgctcg ctcactgagg   1740
ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc   1800
gagcgcgcag agagggacag atccccagga agctcctctg tgtcctcata aaccctaacc   1860
tcctctactt gagaggacat tccaatcata ggctgcccat ccaccctctg tgtcctcctg   1920
ttaattaggt cacttaacaa aaaggaaatt gggtaggggt ttttcacaga ccgctttcta   1980
agggtaattt taaaatatct gggaagtccc ttccactgct gtgttccaga agtgttggta   2040
aacagcccac aaatgtcaac agcagaaaca tacaagctgt cagctttgca caaggttgta   2100
acactggcag agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact   2160
tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca   2220
aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc   2280
tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct   2340
tcttcacgag gcagacctca gcgccccccc cccccggagt caggcaacta tggatgaacg   2400
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca   2460
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta   2520
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca   2580
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg   2640
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga   2700
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa   2760
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc   2820
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg   2880
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac   2940
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct   3000
acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc   3060
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg   3120
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg   3180
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct   3240
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga   3300
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg   3360
cagcgagtca gtgagcgagg aagcggaaga aattctatga tttctctcat tacttcctca   3420
catgttggag gcattttctc tccctctgca cttaatagcc tatcttgctt taatttcttc   3480
ccactcttgg aggctaggtt tggtttggtg ggctacactc cgctatcgct acgtgactgg   3540
gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg   3600
ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg   3660
ttttcaccgt catcaccgaa acgcgcgagg cagctgtgga atgtgtgtca gttagggtgt   3720
ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca   3780
gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat   3840
ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg   3900
cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc   3960
gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta   4020
ggcttttgca aaaagctttc tgacagttac caatgcttaa tcagtgaggc acctatctca   4080
gcgatctgtc tatttcgttc atccatagtt gcctgactcc gggggggggg ggcgctgagg   4140
tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag   4200
ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat   4260
tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc   4320
cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta   4380
atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc   4440
aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt   4500
ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat   4560
cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa   4620
ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa   4680
agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa   4740
tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg   4800
cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact   4860
gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct   4920
gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc   4980
ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta   5040
acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc   5100
ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac   5160
ccatataaat cagcatccat gttggaattt aatcgcggcc tcgaacaaga cgtttcccgt   5220
tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt   5280
catgatgata tatttttatc ttgtgcaatg taacatcaga gattttacgt caaagggcga   5340
acgagttggt tcagctgctg cctgaggctg gacgacctcg cggagttcta ccggcagtgc   5400
aaatccgtcg gcatccagga aaccagcagc ggctatccgc gcatccatgc ccccgaactg   5460
caggagtggg gaggcacgat ggccgctttg gtccggatct ttgtgaagga accttacttc   5520
tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata   5580
aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt   5640
ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg   5700
ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct   5760
actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta   5820
agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc   5880
acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt   5940
ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat   6000
agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt   6060
aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca   6120
taccacattt gtagaggttt tacttgcttt a                                  6151
<210>  5
<211>  352
<212>  DNA
<213>  ˹
<220>
<223>  LP15
<400>  5
gttaattttt aaaaagcaag tggcccttgg cagcatctgt ttgctctggt taataatctc     60
aggagcacaa acattcccag gagaagaaat caacatcctg gacttatcct ctgggcctaa    120
gtatttagtt tggttagtaa ttactaaaca ctgagaacgc caatgaaata caaagatgag    180
tctagttaat aatctacaat tattggttaa agaagtatat tagtgctaat ttccctccgt    240
ttgtcctagc ttttctcttc tgtcaacccc acacgccttt ggcaggtaag ttggcgccgt    300
ttaagggatg gttggttggt ggggtattaa tgtttaatta ccttttttac ag            352
<210>  6
<211>  22
<212>  DNA
<213>  ˹
<220>
<223>  miR-142-3p
<400>  6
tccataaagt aggaaacact ac                                              22
<210>  7
<211>  102
<212>  DNA
<213>  ˹
<220>
<223>  4miR-142-3p
<400>  7
tccataaagt aggaaacact acgatctcca taaagtagga aacactacag tatctccata     60
aagtaggaaa cactacgcta tccataaagt aggaaacact ac                       102
<210>  8
<211>  20
<212>  DNA
<213>  ˹
<220>
<223>  LPL-S447X
<400>  8
tcaaccacag ctccaagacc                                                 20
<210>  9
<211>  19
<212>  DNA
<213>  ˹
<220>
<223>  LPL-S447Xķ
<400>  9
gtatagccgg cggacactg                                                  19
<210>  10
<211>  230
<212>  DNA
<213>  ˹
<220>
<223>  BGH polyA
<400>  10
gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc     60
ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg    120
cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg    180
gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat               230
<210>  11
<211>  29
<212>  DNA
<213>  ˹
<220>
<223>  TBGǿ
<400>  11
gggtctggca gccaaagcaa tcactcaaa                                       29
<210>  12
<211>  2267
<212>  DNA
<213>  ˹
<220>
<223>  ֬֬ø
<400>  12
gttaattttt aaaaagcaag tggcccttgg cagcatctgt ttgctctggt taataatctc     60
aggagcacaa acattcccag gagaagaaat caacatcctg gacttatcct ctgggcctaa    120
gtatttagtt tggttagtaa ttactaaaca ctgagaacgc caatgaaata caaagatgag    180
tctagttaat aatctacaat tattggttaa agaagtatat tagtgctaat ttccctccgt    240
ttgtcctagc ttttctcttc tgtcaacccc acacgccttt ggcaggtaag ttggcgccgt    300
ttaagggatg gttggttggt ggggtattaa tgtttaatta ccttttttac agggtaccgc    360
caccatggag tccaaggccc tgctggtgct gacactggcc gtgtggctgc agtctctgac    420
cgcaagcagg ggaggagtgg cagcagcaga ccagcggaga gacttcatcg atatcgagtc    480
caagtttgca ctgaggaccc cagaggacac agcagaggat acctgccacc tgatccctgg    540
cgtggccgag tctgtggcca catgtcactt caaccacagc tccaagacct tcatggtcat    600
ccacggctgg accgtgacag gtgagtatct cagggatcca gacatgggga tatgggaggt    660
gcctctgatc ccagggctca ctgtgggtct ctctgttcac aggcatgtac gagagctggg    720
tgcctaagct ggtggccgcc ctgtataaga gggagccaga ctctaacgtg atcgtggtgg    780
attggctgag cagggcacag gagcactacc cagtgtccgc cggctatacc aagctggtgg    840
gacaggacgt ggcacggttc atcaattgga tggaggagga gtttaactac cccctggata    900
atgtgcacct gctgggatat tctctgggag cacacgcagc aggaatcgca ggaagcctga    960
caaacaagaa agtgaatagg atcaccggac tggatccagc aggacctaat ttcgagtacg   1020
cagaggcacc tagcaggctg tccccagacg atgccgactt cgtggatgtg ctgcacacct   1080
ttacaagggg atccccaggc cgctctatcg gaatccagaa gcccgtgggc cacgtggaca   1140
tctatcccaa cggcggcacc tttcagcctg gctgcaatat cggcgaggcc atcagagtga   1200
tcgccgagag aggcctgggc gacgtggatc agctggtgaa gtgtagccac gagcggtcca   1260
tccacctgtt catcgattcc ctgctgaacg aggagaatcc ctctaaggcc tacagatgct   1320
ctagcaagga ggcctttgag aagggcctgt gcctgtcctg taggaagaac cgctgtaaca   1380
atctgggcta cgagatcaat aaggtgcggg ccaagagatc ctctaagatg tatctgaaga   1440
cacggagcca gatgccttac aaggtgttcc actatcaggt gaagatccac ttttctggca   1500
ccgagagcga gacccacaca aaccaggcct tcgagatcag cctgtacggc acagtggccg   1560
agtccgagaa catcccattc accctgcccg aggtgtctac caataagaca tacagctttc   1620
tgatctatac agaggtggac atcggcgagc tgctgatgct gaagctgaag tggaagtctg   1680
acagctattt ctcctggtct gattggtgga gctcccctgg ctttgccatc cagaagatcc   1740
gggtgaaggc cggcgagacc cagaagaaag tgatcttctg ctccagagag aaggtgtctc   1800
acctgcagaa gggcaaggcc ccagccgtgt ttgtgaagtg tcacgataag agcctgaata   1860
agaagtgata ataaagtggc cgcgactcta gagggaattc tccataaagt aggaaacact   1920
acgatctcca taaagtagga aacactacag tatctccata aagtaggaaa cactacgcta   1980
tccataaagt aggaaacact acagatctgc ctcgactgtg ccttctagtt gccagccatc   2040
tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct   2100
ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg   2160
gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg   2220
ggatgcggtg ggctctatgg gtctggcagc caaagcaatc actcaaa                 2267
<210>  13
<211>  21
<212>  DNA
<213>  ˹
<220>
<223>  LP15еLP15-F
<400>  13
aagtggccct tggcagcatc t                                               21
<210>  14
<211>  25
<212>  DNA
<213>  ˹
<220>
<223>  LP15еķLP15-R
<400>  14
ggacaaacgg agggaaatta gcact                                           25



