                        б

<110>  ͷ޿ҽҩƼ޹˾
<120>  һCARǶϿԭмӦCAR-NKϸ
<160>  14    
<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  DNA
<213>  ˹

<400>  1
atggcc                                                                   6


<210>  2
<211>  120
<212>  DNA
<213>  ˹

<400>  2
cccaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc       60

ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg      120


<210>  3
<211>  63
<212>  DNA
<213>  ˹

<400>  3
ccattttttt tctgctgctt catcgctgta gccatgggaa tccgtttcat tattatggta       60

aca                                                                     63


<210>  4
<211>  360
<212>  DNA
<213>  ˹

<400>  4
tggaggagaa agaggaagga gaagcagtca gagaccagtc ccaaggaatt tttgacaatt       60

tacgaagatg tcaaggatct gaaaaccagg agaaatcacg agcaggagca gacttttcct      120

ggagggggga gcaccatcta ctctatgatc cagtcccagt cttctgctcc cacgtcacaa      180

gaacctgcat atacattata ttcattaatt cagccttcca ggaagtctgg atccaggaag      240

aggaaccaca gcccttcctt caatagcact atctatgaag tgattggaaa gagtcaacct      300

aaagcccaga accctgctcg attgagccgc aaagagctgg agaactttga tgtttattcc      360


<210>  5
<211>  342
<212>  DNA
<213>  ˹

<400>  5
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgcag agaaggaaga accctcagga aggcctgtac      180

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag      240

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac      300

acctacgacg cccttcacat gcaggccctg ccccctcgct aa                         342


<210>  6
<211>  702
<212>  DNA
<213>  ˹

<400>  6
tggaggagaa agaggaagga gaagcagtca gagaccagtc ccaaggaatt tttgacaatt       60

tacgaagatg tcaaggatct gaaaaccagg agaaatcacg agcaggagca gacttttcct      120

ggagggggga gcaccatcta ctctatgatc cagtcccagt cttctgctcc cacgtcacaa      180

gaacctgcat atacattata ttcattaatt cagccttcca ggaagtctgg atccaggaag      240

aggaaccaca gcccttcctt caatagcact atctatgaag tgattggaaa gagtcaacct      300

aaagcccaga accctgctcg attgagccgc aaagagctgg agaactttga tgtttattcc      360

agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc      420

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      480

cgggaccctg agatgggggg aaagccgcag agaaggaaga accctcagga aggcctgtac      540

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag      600

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac      660

acctacgacg cccttcacat gcaggccctg ccccctcgct aa                         702


<210>  7
<211>  720
<212>  DNA
<213>  ˹

<400>  7
caggtacaac tgcagcagtc tgggcctgag ctggagaagc ctggcgcttc agtgaagata       60

tcctgcaagg cttctggtta ctcattcact ggctacacca tgaactgggt gaagcagagc      120

catggaaaga gccttgagtg gattggactt attactcctt acaatggtgc ttctagctac      180

aaccagaagt tcaggggcaa ggccacatta actgtagaca agtcatccag cacagcctac      240

atggacctcc tcagtctgac atctgaagac tctgcagtct atttctgtgc aagggggggt      300

tacgacggga ggggttttga ctactggggc caagggacca cggtcaccgt ctcctcaggt      360

gtaggcggtt caggcggcgg tggctctggc ggtggcggat cggacatcga gctcactcag      420

tctccagcaa tcatgtctgc atctccaggg gagaaggtca ccatgacctg cagtgccagc      480

tcaagtgtaa gttacatgca ctggtaccag cagaagtcag gcacctcccc caaaagatgg      540

atttatgaca catccaaact ggcttctgga gtcccaggtc gcttcagtgg cagtgggtct      600

ggaaactctt actctctcac aatcagcagc gtggaggctg aagatgatgc aacttattac      660

tgccagcagt ggagtggtta ccctctcacg ttcggtgctg ggacaaagtt ggaaataaaa      720


<210>  8
<211>  1611
<212>  DNA
<213>  ˹

<400>  8
atggcccagg tacaactgca gcagtctggg cctgagctgg agaagcctgg cgcttcagtg       60

aagatatcct gcaaggcttc tggttactca ttcactggct acaccatgaa ctgggtgaag      120

cagagccatg gaaagagcct tgagtggatt ggacttatta ctccttacaa tggtgcttct      180

agctacaacc agaagttcag gggcaaggcc acattaactg tagacaagtc atccagcaca      240

gcctacatgg acctcctcag tctgacatct gaagactctg cagtctattt ctgtgcaagg      300

gggggttacg acgggagggg ttttgactac tggggccaag ggaccacggt caccgtctcc      360

tcaggtgtag gcggttcagg cggcggtggc tctggcggtg gcggatcgga catcgagctc      420

actcagtctc cagcaatcat gtctgcatct ccaggggaga aggtcaccat gacctgcagt      480

gccagctcaa gtgtaagtta catgcactgg taccagcaga agtcaggcac ctcccccaaa      540

agatggattt atgacacatc caaactggct tctggagtcc caggtcgctt cagtggcagt      600

gggtctggaa actcttactc tctcacaatc agcagcgtgg aggctgaaga tgatgcaact      660

tattactgcc agcagtggag tggttaccct ctcacgttcg gtgctgggac aaagttggaa      720

ataaaaccca ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      780

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      840

agggggccat tttttttctg ctgcttcatc gctgtagcca tgggaatccg tttcattatt      900

atggtaacat ggaggagaaa gaggaaggag aagcagtcag agaccagtcc caaggaattt      960

ttgacaattt acgaagatgt caaggatctg aaaaccagga gaaatcacga gcaggagcag     1020

acttttcctg gaggggggag caccatctac tctatgatcc agtcccagtc ttctgctccc     1080

acgtcacaag aacctgcata tacattatat tcattaattc agccttccag gaagtctgga     1140

tccaggaaga ggaaccacag cccttccttc aatagcacta tctatgaagt gattggaaag     1200

agtcaaccta aagcccagaa ccctgctcga ttgagccgca aagagctgga gaactttgat     1260

gtttattcca gagtgaagtt cagcaggagc gcagacgccc ccgcgtacca gcagggccag     1320

aaccagctct ataacgagct caatctagga cgaagagagg agtacgatgt tttggacaag     1380

agacgtggcc gggaccctga gatgggggga aagccgcaga gaaggaagaa ccctcaggaa     1440

ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg     1500

aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc     1560

accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta a              1611


<210>  9
<211>  1713
<212>  DNA
<213>  ˹

<400>  9
atggccctcc ccgtgacagc tctgctgctg cctctggctc ttctgctgca cgccgctaga       60

cctcaggtgc agctgcagca gagcggtcct gagctggaaa aacctggcgc ctccgtcaag      120

atctcatgca aggccagcgg gtactcattc accggctaca ccatgaactg ggtcaagcag      180

agccacggca aaagcctgga atggatcgga ctgattaccc cttacaacgg cgccagcagc      240

tataatcaaa agtttagagg caaggccacc ctgaccgtgg acaagtcctc ttctacagcc      300

tacatggacc tgctgtctct gacatctgag gatagcgccg tgtacttctg cgccagaggc      360

ggatatgatg gcagaggctt cgactactgg ggacagggca caaccgtgac cgtcagctcc      420

ggggtgggcg gctctggagg cggtggctct ggcgggggtg gcagtgatat cgagctgacc      480

cagagccccg ccatcatgag cgcctctcct ggcgagaagg tgaccatgac ctgtagcgct      540

agcagcagtg tgtcctacat gcactggtac cagcagaaaa gcggcacctc cccaaagaga      600

tggatctacg acacctcaaa gctggcctct ggcgtgcccg gacggttcag cgggtctggc      660

agcggcaaca gctactccct gaccatcagc tccgtggaag ccgaggacga cgccacatat      720

tactgccagc aatggagcgg ataccccctg accttcggcg ccggcaccaa gctggaaatc      780

aagttcgtgc ctgtgttcct gcctgccaag cccacaacaa cccctgcccc tcggcctcca      840

acacctgctc ctaccatcgc cagccagcct ctgagcctga gaccagaggc ctgtagacct      900

gccgccggag gcgctgtgca caccagagga ctggacttcg cctgcgaccc cttcttcttt      960

tgctgcttca tcgccgtggc catgggcatc agattcatca tcatggtgac ctggcggaga     1020

aagcggaaag agaaacagag cgagacaagc cctaaggaat tcctgaccat ctatgaggac     1080

gtgaaggacc tgaagaccag acggaaccac gagcaggagc agacctttcc aggcggcgga     1140

agcaccatct acagcatgat ccaaagccag tctagtgccc ctacaagcca ggagcctgcc     1200

tatacactgt acagcctcat ccagcccagc agaaagagcg gttccagaaa gagaaaccac     1260

agccctagct tcaacagcac aatctacgag gtgatcggta agtcccagcc taaggcccag     1320

aatcctgcta gactgtccag aaaagaactc gaaaacttcg acgtgtactc tagagtgaag     1380

ttcagccgga gcgctgatgc ccctgcttac cagcagggcc agaaccagct gtacaacgag     1440

ctgaacctgg gcagacggga agagtacgat gtgctggata agcggagagg cagagatcct     1500

gagatgggcg gcaagcctca aagacggaag aacccccagg agggcctgta caatgagctg     1560

cagaaagaca agatggccga agcctacagc gagatcggca tgaaaggcga gagacgacgg     1620

ggcaagggcc acgacggcct gtatcagggc ctgagcaccg ccacaaagga tacatacgac     1680

gccctccaca tgcaggccct gccccccaga tga                                  1713


<210>  10
<211>  536
<212>  PRT
<213>  ˹

<400>  10

Met Ala Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr 
            20                  25                  30          


Gly Tyr Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu 
        35                  40                  45              


Trp Ile Gly Leu Ile Thr Pro Tyr Asn Gly Ala Ser Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Arg Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Asp Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
                85                  90                  95      


Phe Cys Ala Arg Gly Gly Tyr Asp Gly Arg Gly Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Val Thr Val Ser Ser Gly Val Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser 
145                 150                 155                 160 


Ala Ser Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Ser Gly 
                165                 170                 175     


Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly 
            180                 185                 190         


Val Pro Gly Arg Phe Ser Gly Ser Gly Ser Gly Asn Ser Tyr Ser Leu 
        195                 200                 205             


Thr Ile Ser Ser Val Glu Ala Glu Asp Asp Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Trp Ser Gly Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Pro Phe Phe Phe Cys Cys 
        275                 280                 285             


Phe Ile Ala Val Ala Met Gly Ile Arg Phe Ile Ile Met Val Thr Trp 
    290                 295                 300                 


Arg Arg Lys Arg Lys Glu Lys Gln Ser Glu Thr Ser Pro Lys Glu Phe 
305                 310                 315                 320 


Leu Thr Ile Tyr Glu Asp Val Lys Asp Leu Lys Thr Arg Arg Asn His 
                325                 330                 335     


Glu Gln Glu Gln Thr Phe Pro Gly Gly Gly Ser Thr Ile Tyr Ser Met 
            340                 345                 350         


Ile Gln Ser Gln Ser Ser Ala Pro Thr Ser Gln Glu Pro Ala Tyr Thr 
        355                 360                 365             


Leu Tyr Ser Leu Ile Gln Pro Ser Arg Lys Ser Gly Ser Arg Lys Arg 
    370                 375                 380                 


Asn His Ser Pro Ser Phe Asn Ser Thr Ile Tyr Glu Val Ile Gly Lys 
385                 390                 395                 400 


Ser Gln Pro Lys Ala Gln Asn Pro Ala Arg Leu Ser Arg Lys Glu Leu 
                405                 410                 415     


Glu Asn Phe Asp Val Tyr Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
            420                 425                 430         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        435                 440                 445             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    450                 455                 460                 


Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
465                 470                 475                 480 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                485                 490                 495     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            500                 505                 510         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        515                 520                 525             


His Met Gln Ala Leu Pro Pro Arg 
    530                 535     


<210>  11
<211>  63
<212>  DNA
<213>  ˹

<400>  11
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210>  12
<211>  720
<212>  DNA
<213>  ˹

<400>  12
caggtgcagc tggtgcagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctctggatt caccttcagt agctatggca tgcactgggt ccgccaggct      120

ccaggcaagg ggctggagtg ggtggcagtt atatcatatg atggaagtaa taaatactac      180

gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agctgaggac acggctgtgt actactgtgc gagagattgg      300

ggggactcac tggatccctg gggcaaaggc accctggtca ccgtctcgag tggtggaggc      360

ggttcaggcg gaggtggcag cggcggtggc ggatcggaca tccagatgac ccagtctcct      420

tccaccctgt ctgcatctat tggagacaga gtcaccatca cctgccgggc cagtgagggt      480

atttatcgct ggttggcctg gtatcagcag aagccaggga aagcccctaa actcctgatc      540

tataaggcct ctagtttagc cagtcgggcc ccatcaaggt tcagcggcag tggatctggg      600

acagatttca ctctcaccat cagcagcctg cagcctgatg attttgcaac ttattactgc      660

caacaatata gtaattatcc gctcactttc ggcggaggga ccaagctgga gatcaaacgt      720


<210>  13
<211>  1521
<212>  DNA
<213>  ˹

<400>  13
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgcaggtgc agctggtgca gtctggggga ggcttggtac agcctggggg gtccctgaga      120

ctctcctgtg cagcctctgg attcaccttc agtagctatg gcatgcactg ggtccgccag      180

gctccaggca aggggctgga gtgggtggca gttatatcat atgatggaag taataaatac      240

tacgcagact ccgtgaaggg ccgattcacc atctccagag acaattccaa gaacacgctg      300

tatctgcaaa tgaacagcct gagagctgag gacacggctg tgtactactg tgcgagagat      360

tggggggact cactggatcc ctggggcaaa ggcaccctgg tcaccgtctc gagtggtgga      420

ggcggttcag gcggaggtgg cagcggcggt ggcggatcgg acatccagat gacccagtct      480

ccttccaccc tgtctgcatc tattggagac agagtcacca tcacctgccg ggccagtgag      540

ggtatttatc gctggttggc ctggtatcag cagaagccag ggaaagcccc taaactcctg      600

atctataagg cctctagttt agccagtcgg gccccatcaa ggttcagcgg cagtggatct      660

gggacagatt tcactctcac catcagcagc ctgcagcctg atgattttgc aacttattac      720

tgccaacaat atagtaatta tccgctcact ttcggcggag ggaccaagct ggagatcaaa      780

cgtccatttt ttttctgctg cttcatcgct gtagccatgg gaatccgttt cattattatg      840

gtaacatgga ggagaaagag gaaggagaag cagtcagaga ccagtcccaa ggaatttttg      900

acaatttacg aagatgtcaa ggatctgaaa accaggagaa atcacgagca ggagcagact      960

tttcctggag gggggagcac catctactct atgatccagt cccagtcttc tgctcccacg     1020

tcacaagaac ctgcatatac attatattca ttaattcagc cttccaggaa gtctggatcc     1080

aggaagagga accacagccc ttccttcaat agcactatct atgaagtgat tggaaagagt     1140

caacctaaag cccagaaccc tgctcgattg agccgcaaag agctggagaa ctttgatgtt     1200

tattccgccc ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga     1260

cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga     1320

aagccgcaga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag     1380

atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac     1440

gatggccttt accagggtct cagtacagcc accaaggaca cctacgacgc ccttcacatg     1500

caggccctgc cccctcgcta a                                               1521


<210>  14
<211>  506
<212>  PRT
<213>  ˹

<400>  14

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Asp Trp Gly Asp Ser Leu Asp Pro Trp 
        115                 120                 125             


Gly Lys Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Thr Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     


Arg Ala Ser Glu Gly Ile Tyr Arg Trp Leu Ala Trp Tyr Gln Gln Lys 
            180                 185                 190         


Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser Ser Leu Ala 
        195                 200                 205             


Ser Arg Ala Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 


Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Gln Gln Tyr Ser Asn Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys 
                245                 250                 255     


Leu Glu Ile Lys Arg Pro Phe Phe Phe Cys Cys Phe Ile Ala Val Ala 
            260                 265                 270         


Met Gly Ile Arg Phe Ile Ile Met Val Thr Trp Arg Arg Lys Arg Lys 
        275                 280                 285             


Glu Lys Gln Ser Glu Thr Ser Pro Lys Glu Phe Leu Thr Ile Tyr Glu 
    290                 295                 300                 


Asp Val Lys Asp Leu Lys Thr Arg Arg Asn His Glu Gln Glu Gln Thr 
305                 310                 315                 320 


Phe Pro Gly Gly Gly Ser Thr Ile Tyr Ser Met Ile Gln Ser Gln Ser 
                325                 330                 335     


Ser Ala Pro Thr Ser Gln Glu Pro Ala Tyr Thr Leu Tyr Ser Leu Ile 
            340                 345                 350         


Gln Pro Ser Arg Lys Ser Gly Ser Arg Lys Arg Asn His Ser Pro Ser 
        355                 360                 365             


Phe Asn Ser Thr Ile Tyr Glu Val Ile Gly Lys Ser Gln Pro Lys Ala 
    370                 375                 380                 


Gln Asn Pro Ala Arg Leu Ser Arg Lys Glu Leu Glu Asn Phe Asp Val 
385                 390                 395                 400 


Tyr Ser Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
                405                 410                 415     


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
            420                 425                 430         


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro 
        435                 440                 445             


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
    450                 455                 460                 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
465                 470                 475                 480 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
                485                 490                 495     


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505     


