                         SEQUENCE LISTING

<110>  Centro de Investigacin y de Estudios Avanzados del I.P.N.
 
<120>  Vacunas para prevenir la COVID19 basadas en sitios no 
       glicosilados del dominio de unin al receptor (RBD) de la 
       protena S del virus SARS-CoV-2

<130>  Cinvestav46

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  50
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Protena NG06

<220>
<221>  MISC_FEATURE
<223>  Fragmento S438-N487 del dominio de unin al receptor (RBD) de la 
       protena S del virus SARS-CoV-2

<400>  1

Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr 
1               5                   10                  15      


Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser 
            20                  25                  30          


Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly 
        35                  40                  45              


Phe Asn 
    50  


<210>  2
<211>  171
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Protena NG19

<220>
<221>  MISC_FEATURE
<223>  Fragmento S371-F541 del dominio de unin al receptor (RBD) de la 
       protena S del virus SARS-CoV-2

<400>  2

Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
1               5                   10                  15      


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
            20                  25                  30          


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
        35                  40                  45              


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
    50                  55                  60                  


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
65                  70                  75                  80  


Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
                85                  90                  95      


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
            100                 105                 110         


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
        115                 120                 125             


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
    130                 135                 140                 


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
145                 150                 155                 160 


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
                165                 170     


<210>  3
<211>  150
<212>  DNA
<213>  SARS-CoV-2


<220>
<221>  misc_feature
<223>  Secuencia codificadora de la protena NG06

<220>
<221>  CDS
<222>  (1)..(150)

<400>  3
agc aac aac ctg gat agc aaa gtc ggc ggt aac tac aac tac ctg tac         48
Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr           
1               5                   10                  15                

cgc ctg ttc cgc aaa agc aac ctg aaa ccg ttc gaa cgc gac atc agc         96
Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser           
            20                  25                  30                    

acc gaa att tat cag gcc ggt tct acc ccg tgt aac ggc gtt gaa ggt        144
Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly           
        35                  40                  45                        

ttt aac                                                                150
Phe Asn                                                                   
    50                                                                    


<210>  4
<211>  50
<212>  PRT
<213>  SARS-CoV-2

<400>  4

Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr 
1               5                   10                  15      


Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser 
            20                  25                  30          


Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly 
        35                  40                  45              


Phe Asn 
    50  


<210>  5
<211>  516
<212>  DNA
<213>  SARS-CoV-2


<220>
<221>  misc_feature
<223>  Secuencia codificadora de la protena NG19

<220>
<221>  CDS
<222>  (1)..(516)

<400>  5
agc gcg agc ttc tcc acc ttc aaa tgc tat ggc gtt agt ccg acc aaa         48
Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys           
1               5                   10                  15                

ctg aac gat ctg tgc ttc acc aac gtc tac gca gat agc ttt gta att         96
Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile           
            20                  25                  30                    

cgc ggc gac gaa gtt cgt cag att gca ccg ggt caa acc ggc aaa att        144
Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile           
        35                  40                  45                        

gcg gac tac aac tac aaa ctg ccg gac gat ttt acc ggt tgc gtt att        192
Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile           
    50                  55                  60                            

gcc tgg aac agc aac aac ctg gat agc aaa gtc ggc ggt aac tac aac        240
Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn           
65                  70                  75                  80            

tac ctg tac cgc ctg ttc cgc aaa agc aac ctg aaa ccg ttc gaa cgc        288
Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg           
                85                  90                  95                

gac atc agc acc gaa att tat cag gcc ggt tct acc ccg tgt aac ggc        336
Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly           
            100                 105                 110                   

gtt gaa ggt ttt aac tgc tac ttt ccg ctg cag agc tac ggt ttt cag        384
Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln           
        115                 120                 125                       

ccg acc aac ggc gta ggt tat caa ccg tat cgc gtt gtt gtt ctg agc        432
Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser           
    130                 135                 140                           

ttt gaa ctg ctg cat gca ccg gca acc gtt tgc ggt ccg aaa aaa tcc        480
Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser           
145                 150                 155                 160           

acc aac ctg gtc aaa aac aaa tgc gtc aac ttc taa                        516
Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe                               
                165                 170                                   


<210>  6
<211>  171
<212>  PRT
<213>  SARS-CoV-2

<400>  6

Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
1               5                   10                  15      


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
            20                  25                  30          


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
        35                  40                  45              


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
    50                  55                  60                  


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
65                  70                  75                  80  


Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
                85                  90                  95      


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
            100                 105                 110         


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
        115                 120                 125             


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
    130                 135                 140                 


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
145                 150                 155                 160 


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
                165                 170     


<210>  7
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttica


<220>
<221>  MISC_FEATURE
<223>  Protena His-Tag-NG06

<220>
<221>  MISC_FEATURE
<223>  aa 1-13 His-Tag

<220>
<221>  MISC_FEATURE
<223>  aa 14-19 sitio TEV

<220>
<221>  MISC_FEATURE
<223>  aa 20-23 adicionales del vector

<220>
<221>  MISC_FEATURE
<223>  aa 24-73 protena NG06

<400>  7

Met Gly Ser Ser His His His His His His Ser Gly Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Ala Met Ala Ser Asn Asn Leu Asp Ser Lys Val Gly 
            20                  25                  30          


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        35                  40                  45              


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    50                  55                  60                  


Pro Cys Asn Gly Val Glu Gly Phe Asn 
65                  70              


<210>  8
<211>  565
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttica


<220>
<221>  MISC_FEATURE
<223>  Protena His-Tag-NG19

<220>
<221>  MISC_FEATURE
<223>  aa 1-8 His-Tag

<220>
<221>  MISC_FEATURE
<223>  aa 9-384 MBP

<220>
<221>  MISC_FEATURE
<223>  aa 385-390 sitio TEV

<220>
<221>  MISC_FEATURE
<223>  aa 391-394 adicionales del vector

<220>
<221>  MISC_FEATURE
<223>  aa 395-565 protena NG19

<400>  8

Met Lys His His His His His His Pro Met Lys Ile Glu Glu Gly Lys 
1               5                   10                  15      


Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu 
            20                  25                  30          


Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu 
        35                  40                  45              


His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly 
    50                  55                  60                  


Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr 
65                  70                  75                  80  


Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln 
                85                  90                  95      


Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys 
            100                 105                 110         


Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn 
        115                 120                 125             


Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala 
    130                 135                 140                 


Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn 
145                 150                 155                 160 


Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly 
                165                 170                 175     


Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly 
            180                 185                 190         


Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu 
        195                 200                 205             


Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu 
    210                 215                 220                 


Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp 
225                 230                 235                 240 


Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val 
                245                 250                 255     


Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu 
            260                 265                 270         


Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu 
        275                 280                 285             


Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn 
    290                 295                 300                 


Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu 
305                 310                 315                 320 


Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys 
                325                 330                 335     


Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala 
            340                 345                 350         


Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp 
        355                 360                 365             


Glu Ala Leu Lys Asp Ala Gln Thr Asn Ser Gly Ser Gly Ser Gly Ser 
    370                 375                 380                 


Glu Asn Leu Tyr Phe Gln Gly Ala Met Ala Ser Ala Ser Phe Ser Thr 
385                 390                 395                 400 


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                405                 410                 415     


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            420                 425                 430         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        435                 440                 445             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    450                 455                 460                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
465                 470                 475                 480 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                485                 490                 495     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            500                 505                 510         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        515                 520                 525             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    530                 535                 540                 


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
545                 550                 555                 560 


Lys Cys Val Asn Phe 
                565 


<210>  9
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttica


<220>
<221>  misc_feature
<223>  Secuencia codificadora para la Protena His-Tag-NG06

<400>  9
atgggcagca gccatcatca tcatcatcac agcagcggcg aaaaccttta cttccagggc       60

gccatggcca gcaacaacct ggatagcaaa gtcggcggta actacaacta cctgtaccgc      120

ctgttccgca aaagcaacct gaaaccgttc gaacgcgaca tcagcaccga aatttatcag      180

gccggttcta ccccgtgtaa cggcgttgaa ggttttaac                             219


<210>  10
<211>  1698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Secuencia codificadora para la Protena His-Tag-NG19

<400>  10
atgaaacatc accatcacca tcaccccatg aaaatcgaag aaggtaaact ggtaatctgg       60

attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat      120

accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt      180

gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac      240

gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat      300

ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt      360

gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa      420

gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac      480

ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag      540

tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg      600

ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac      660

tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg      720

gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc      780

aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt      840

ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg      900

gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag      960

ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg     1020

ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc     1080

gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgggatct     1140

ggcagtggtt ctgagaatct ttattttcag ggcgccatgg ccagcgcgag cttctccacc     1200

ttcaaatgct atggcgttag tccgaccaaa ctgaacgatc tgtgcttcac caacgtctac     1260

gcagatagct ttgtaattcg cggcgacgaa gttcgtcaga ttgcaccggg tcaaaccggc     1320

aaaattgcgg actacaacta caaactgccg gacgatttta ccggttgcgt tattgcctgg     1380

aacagcaaca acctggatag caaagtcggc ggtaactaca actacctgta ccgcctgttc     1440

cgcaaaagca acctgaaacc gttcgaacgc gacatcagca ccgaaattta tcaggccggt     1500

tctaccccgt gtaacggcgt tgaaggtttt aactgctact ttccgctgca gagctacggt     1560

tttcagccga ccaacggcgt aggttatcaa ccgtatcgcg ttgttgttct gagctttgaa     1620

ctgctgcatg caccggcaac cgtttgcggt ccgaaaaaat ccaccaacct ggtcaaaaac     1680

aaatgcgtca acttctaa                                                   1698


<210>  11
<211>  7
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 1 de la protena NG06

<400>  11

Tyr Asn Tyr Leu Tyr Arg Leu 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 2 de la protena NG06

<400>  12

Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
1               5                   


<210>  13
<211>  15
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 1 de la protena NG19

<400>  13

Ala Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
1               5                   10                  15  


<210>  14
<211>  18
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 2 de la protena NG19

<400>  14

Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val 
1               5                   10                  15      


Ile Arg 
        


<210>  15
<211>  8
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 3 de la protena NG19

<400>  15

Asp Phe Thr Gly Cys Val Ile Ala 
1               5               


<210>  16
<211>  7
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 4 de la protena NG19

<400>  16

Tyr Asn Tyr Leu Tyr Arg Leu 
1               5           


<210>  17
<211>  9
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 5 de la protena NG19

<400>  17

Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
1               5                   


<210>  18
<211>  13
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 6 de la protena NG19

<400>  18

Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
1               5                   10              


<210>  19
<211>  25
<212>  PRT
<213>  SARS-CoV-2


<220>
<221>  MISC_FEATURE
<223>  Eptope 7 de la protena NG19

<400>  19

Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu 
1               5                   10                  15      


His Ala Pro Ala Thr Val Cys Gly Pro 
            20                  25  


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo delantero NcoI-eNG06-F

<400>  20
catgccatgg ccagcaacaa cctggatagc aaag                                   34


<210>  21
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo reverso HindIII-eNG06-R

<400>  21
cccaagcttt tagttaaaac cttcaacgcc gttac                                  35


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo delantero NcoI-eNG19-F

<400>  22
catgccatgg ccagcgcgag cttctccacc ttc                                    33


<210>  23
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo reverso HindIII-eNG19-R

<400>  23
cccaagcttt tagaagttga cgcatttgtt tttg                                   34


<210>  24
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  MISC_FEATURE
<223>  Protena eNG19m

<220>
<221>  MISC_FEATURE
<223>  Fragmento S371-F541 del dominio de unin al receptor (RBD) de la 
       protena S del virus SARS-CoV-2, adyuvado en los extremos amino y
       carboxilo

<400>  24

Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
1               5                   10                  15      


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            20                  25                  30          


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        35                  40                  45              


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    50                  55                  60                  


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
65                  70                  75                  80  


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                85                  90                  95      


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            100                 105                 110         


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        115                 120                 125             


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    130                 135                 140                 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
145                 150                 155                 160 


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp 
                165                 170                 175     


Cys Glu Cys 
            


<210>  25
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  MISC_FEATURE
<223>  Protena eNG19m(20H)

<220>
<221>  MISC_FEATURE
<223>  Fragmento S371-F541 del dominio de unin al receptor (RBD) de la 
       protena S del virus SARS-CoV-2, adyuvado en los extremos amino y
       carboxilo y modificado conforme a las mutaciones de las variantes
       virales Inglesa y Sudafricana

<400>  25

Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
1               5                   10                  15      


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            20                  25                  30          


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        35                  40                  45              


Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    50                  55                  60                  


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
65                  70                  75                  80  


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                85                  90                  95      


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            100                 105                 110         


Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        115                 120                 125             


Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    130                 135                 140                 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
145                 150                 155                 160 


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp 
                165                 170                 175     


Cys Glu Cys 
            


<210>  26
<211>  540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Secuencia codificadora para la protena eNG19m

<220>
<221>  CDS
<222>  (1)..(540)

<400>  26
atg gcc ctt agc gcg agc ttc tcc acc ttc aaa tgc tat ggc gtt agt         48
Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser           
1               5                   10                  15                

ccg acc aaa ctg aac gat ctg tgc ttc acc aac gtc tac gca gat agc         96
Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser           
            20                  25                  30                    

ttt gta att cgc ggc gac gaa gtt cgt cag att gca ccg ggt caa acc        144
Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr           
        35                  40                  45                        

ggc aaa att gcg gac tac aac tac aaa ctg ccg gac gat ttt acc ggt        192
Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly           
    50                  55                  60                            

tgc gtt att gcc tgg aac agc aac aac ctg gat agc aaa gtc ggc ggt        240
Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly           
65                  70                  75                  80            

aac tac aac tac ctg tac cgc ctg ttc cgc aaa agc aac ctg aaa ccg        288
Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro           
                85                  90                  95                

ttc gaa cgc gac atc agc acc gaa att tat cag gcc ggt tct acc ccg        336
Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro           
            100                 105                 110                   

tgt aac ggc gtt gaa ggt ttt aac tgc tac ttt ccg ctg cag agc tac        384
Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr           
        115                 120                 125                       

ggt ttt cag ccg acc aac ggc gta ggt tat caa ccg tat cgc gtt gtt        432
Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val           
    130                 135                 140                           

gtt ctg agc ttt gaa ctg ctg cat gca ccg gca acc gtt tgc ggt ccg        480
Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro           
145                 150                 155                 160           

aaa aaa tcc acc aac ctg gtc aaa aac aaa tgc gtc aac ttc aaa tgg        528
Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp           
                165                 170                 175               

tgc gaa tgc taa                                                        540
Cys Glu Cys                                                               
                                                                          


<210>  27
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27

Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
1               5                   10                  15      


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            20                  25                  30          


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        35                  40                  45              


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    50                  55                  60                  


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
65                  70                  75                  80  


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                85                  90                  95      


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            100                 105                 110         


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        115                 120                 125             


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    130                 135                 140                 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
145                 150                 155                 160 


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp 
                165                 170                 175     


Cys Glu Cys 
            


<210>  28
<211>  540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Secuencia codificadora para la protena eNG19m(20H)

<220>
<221>  CDS
<222>  (1)..(540)

<400>  28
atg gcc ctt agc gcg agc ttc tcc acc ttc aaa tgc tat ggc gtt agt         48
Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser           
1               5                   10                  15                

ccg acc aaa ctg aac gat ctg tgc ttc acc aac gtc tac gca gat agc         96
Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser           
            20                  25                  30                    

ttt gta att cgc ggc gac gaa gtt cgt cag att gca ccg ggt caa acc        144
Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr           
        35                  40                  45                        

ggc aac att gcg gac tac aac tac aaa ctg ccg gac gat ttt acc ggt        192
Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly           
    50                  55                  60                            

tgc gtt att gcc tgg aac agc aac aac ctg gat agc aaa gtc ggc ggt        240
Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly           
65                  70                  75                  80            

aac tac aac tac ctg tac cgc ctg ttc cgc aaa agc aac ctg aaa ccg        288
Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro           
                85                  90                  95                

ttc gaa cgc gac atc agc acc gaa att tat cag gcc ggt tct acc ccg        336
Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro           
            100                 105                 110                   

tgt aac ggc gtt aaa ggt ttt aac tgc tac ttt ccg ctg cag agc tac        384
Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr           
        115                 120                 125                       

ggt ttt cag ccg acc tac ggc gta ggt tat caa ccg tat cgc gtt gtt        432
Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val           
    130                 135                 140                           

gtt ctg agc ttt gaa ctg ctg cat gca ccg gca acc gtt tgc ggt ccg        480
Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro           
145                 150                 155                 160           

aaa aaa tcc acc aac ctg gtc aaa aac aaa tgc gtc aac ttc aag tgg        528
Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp           
                165                 170                 175               

tgc gag tgt taa                                                        540
Cys Glu Cys                                                               
                                                                          


<210>  29
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  29

Met Ala Leu Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
1               5                   10                  15      


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            20                  25                  30          


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        35                  40                  45              


Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    50                  55                  60                  


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
65                  70                  75                  80  


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                85                  90                  95      


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            100                 105                 110         


Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        115                 120                 125             


Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    130                 135                 140                 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
145                 150                 155                 160 


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Lys Trp 
                165                 170                 175     


Cys Glu Cys 
            


<210>  30
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo delantero eNG19m K417N

<400>  30
gtcaaaccgg caacattgcg gactac                                            26


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo reverso eNG19m K417N

<400>  31
gtagtccgca atgttgccgg tttgac                                            26


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo delantero eNG19m E484K

<400>  32
gtgtaacggc gttaaaggtt ttaactg                                           27


<210>  33
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo reverso eNG19m E484K

<400>  33
cagttaaaac ctttaacgcc gttacac                                           27


<210>  34
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo delantero eNG19m N501Y

<400>  34
gttttcagcc gacctacggc gtaggtt                                           27


<210>  35
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  Oligo reverso eNG19m N501Y

<400>  35
aacctacgcc gtaggtcggc tgaaaac                                           27


