                         SEQUENCE LISTING

<110>  Universiteit Antwerpen
 
<120>  METHOD FOR SCREENING POTENTIAL THERAPEUTIC AGENTS

<130>  UA-021-PCT

<150>  EP21162636.1
<151>  2021-03-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HiBiT tag

<400>  1

Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser 
1               5                   10      


<210>  2
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HaloTag polypeptide

<400>  2

Met Ala Glu Ile Gly Thr Gly Phe Pro Phe Asp Pro His Tyr Val Glu 
1               5                   10                  15      


Val Leu Gly Glu Arg Met His Tyr Val Asp Val Gly Pro Arg Asp Gly 
            20                  25                  30          


Thr Pro Val Leu Phe Leu His Gly Asn Pro Thr Ser Ser Tyr Val Trp 
        35                  40                  45              


Arg Asn Ile Ile Pro His Val Ala Pro Thr His Arg Cys Ile Ala Pro 
    50                  55                  60                  


Asp Leu Ile Gly Met Gly Lys Ser Asp Lys Pro Asp Leu Gly Tyr Phe 
65                  70                  75                  80  


Phe Asp Asp His Val Arg Phe Met Asp Ala Phe Ile Glu Ala Leu Gly 
                85                  90                  95      


Leu Glu Glu Val Val Leu Val Ile His Asp Trp Gly Ser Ala Leu Gly 
            100                 105                 110         


Phe His Trp Ala Lys Arg Asn Pro Glu Arg Val Lys Gly Ile Ala Phe 
        115                 120                 125             


Met Glu Phe Ile Arg Pro Ile Pro Thr Trp Asp Glu Trp Pro Glu Phe 
    130                 135                 140                 


Ala Arg Glu Thr Phe Gln Ala Phe Arg Thr Thr Asp Val Gly Arg Lys 
145                 150                 155                 160 


Leu Ile Ile Asp Gln Asn Val Phe Ile Glu Gly Thr Leu Pro Met Gly 
                165                 170                 175     


Val Val Arg Pro Leu Thr Glu Val Glu Met Asp His Tyr Arg Glu Pro 
            180                 185                 190         


Phe Leu Asn Pro Val Asp Arg Glu Pro Leu Trp Arg Phe Pro Asn Glu 
        195                 200                 205             


Leu Pro Ile Ala Gly Glu Pro Ala Asn Ile Val Ala Leu Val Glu Glu 
    210                 215                 220                 


Tyr Met Asp Trp Leu His Gln Ser Pro Val Pro Lys Leu Leu Phe Trp 
225                 230                 235                 240 


Gly Thr Pro Gly Val Leu Ile Pro Pro Ala Glu Ala Ala Arg Leu Ala 
                245                 250                 255     


Lys Ser Leu Pro Asn Cys Lys Ala Val Asp Ile Gly Pro Gly Leu Asn 
            260                 265                 270         


Leu Leu Gln Glu Asp Asn Pro Asp Leu Ile Gly Ser Glu Ile Ala Arg 
        275                 280                 285             


Trp Leu Ser Thr Leu Glu Ile Ser Gly 
    290                 295         


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HH linker peptide

<400>  3

Val Ser Gln Gly Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly 
        


<210>  4
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HaloTag- HiBiT fusion protein

<400>  4

Met Ala Glu Ile Gly Thr Gly Phe Pro Phe Asp Pro His Tyr Val Glu 
1               5                   10                  15      


Val Leu Gly Glu Arg Met His Tyr Val Asp Val Gly Pro Arg Asp Gly 
            20                  25                  30          


Thr Pro Val Leu Phe Leu His Gly Asn Pro Thr Ser Ser Tyr Val Trp 
        35                  40                  45              


Arg Asn Ile Ile Pro His Val Ala Pro Thr His Arg Cys Ile Ala Pro 
    50                  55                  60                  


Asp Leu Ile Gly Met Gly Lys Ser Asp Lys Pro Asp Leu Gly Tyr Phe 
65                  70                  75                  80  


Phe Asp Asp His Val Arg Phe Met Asp Ala Phe Ile Glu Ala Leu Gly 
                85                  90                  95      


Leu Glu Glu Val Val Leu Val Ile His Asp Trp Gly Ser Ala Leu Gly 
            100                 105                 110         


Phe His Trp Ala Lys Arg Asn Pro Glu Arg Val Lys Gly Ile Ala Phe 
        115                 120                 125             


Met Glu Phe Ile Arg Pro Ile Pro Thr Trp Asp Glu Trp Pro Glu Phe 
    130                 135                 140                 


Ala Arg Glu Thr Phe Gln Ala Phe Arg Thr Thr Asp Val Gly Arg Lys 
145                 150                 155                 160 


Leu Ile Ile Asp Gln Asn Val Phe Ile Glu Gly Thr Leu Pro Met Gly 
                165                 170                 175     


Val Val Arg Pro Leu Thr Glu Val Glu Met Asp His Tyr Arg Glu Pro 
            180                 185                 190         


Phe Leu Asn Pro Val Asp Arg Glu Pro Leu Trp Arg Phe Pro Asn Glu 
        195                 200                 205             


Leu Pro Ile Ala Gly Glu Pro Ala Asn Ile Val Ala Leu Val Glu Glu 
    210                 215                 220                 


Tyr Met Asp Trp Leu His Gln Ser Pro Val Pro Lys Leu Leu Phe Trp 
225                 230                 235                 240 


Gly Thr Pro Gly Val Leu Ile Pro Pro Ala Glu Ala Ala Arg Leu Ala 
                245                 250                 255     


Lys Ser Leu Pro Asn Cys Lys Ala Val Asp Ile Gly Pro Gly Leu Asn 
            260                 265                 270         


Leu Leu Gln Glu Asp Asn Pro Asp Leu Ile Gly Ser Glu Ile Ala Arg 
        275                 280                 285             


Trp Leu Ser Thr Leu Glu Ile Ser Gly Val Ser Gln Gly Ser Ser Gly 
    290                 295                 300                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Val Ser Gly Trp Arg 
305                 310                 315                 320 


Leu Phe Lys Lys Ile Ser 
                325     


<210>  5
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Red fluorescent protein

<400>  5

Met Arg Ser Ser Lys Asn Val Ile Lys Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Ile His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ser Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  6
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Green fluorescent protein

<400>  6

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  7
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  7

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  8
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide

<400>  8

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


