                         SEQUENCE LISTING

<110>  The Board of Regents of the University of Texas System
 
<120>  METHODS FOR RECONSTITUTING T CELL SELECTION AND USES THEREOF

<130>  426871-000252

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (out-of-frame prevents 
       expression)

<400>  1
gtgccagcag cgaaaggggt cgagcagtat                                        30


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (out-of-frame prevents 
       expression)

<400>  2

Cys Ala Ser Ser Glu Gln Tyr 
1               5           


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (stop codon prevents 
       expression)

<400>  3
gtgcaagtcg ctagagggaa ctccggaagc                                        30


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (stop codon prevents 
       expression)

<400>  4

Cys Ala Ser Arg Arg Glu Thr Pro Glu Ala 
1               5                   10  


<210>  5
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (2nd V(D)J recombination 
       event)

<400>  5
gtgccagccg gacctcccac ggcta                                             25


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (2nd V(D)J recombination 
       event)

<400>  6

Cys Ala Ser Arg Thr Ser His Gly Tyr 
1               5                   


<210>  7
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to C)

<400>  7
tgtgccagca gtgacgggac tcagtac                                           27


<210>  8
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete C)

<400>  8
gtgccagcag gaaaggggtc gagcagtat                                         29


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete C)

<400>  9

Cys Ala Ser Arg Lys Gly Val Glu Gln Tyr 
1               5                   10  


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete G)

<400>  10
gtgccagcag caaaggggtc gagcagtat                                         29


<210>  11
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete G)

<400>  11

Cys Ala Ser Ser Lys Gly Val Glu Gln Tyr 
1               5                   10  


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete A)

<400>  12
gtgccagcag cgaaggggtc gagcagtat                                         29


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete A)

<400>  13

Cys Ala Ser Ser Glu Gly Val Glu Gln Tyr 
1               5                   10  


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete A)

<400>  14
gtgccagcag cgaaggggtc gagcagtat                                         29


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to C)

<400>  15

Cys Ala Ser Ser Asp Gly Thr Gln Tyr 
1               5                   


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete T)

<400>  16
gtgccagcag cgaaaggggc gagcagtat                                         29


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (delete T)

<400>  17

Cys Ala Ser Ser Glu Arg Gly Glu Gln Tyr 
1               5                   10  


<210>  18
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (stop codon prevents 
       expression)

<400>  18
gtgcaagtcg ctagagggaa actccggaag cttt                                   34


<210>  19
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR gene (stop codon prevents 
       expression)

<400>  19

Cys Ala Ser Arg Arg Glu Thr Pro Glu Ala Phe 
1               5                   10      


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to C)

<400>  20
gtgcaagtcg ctcgagggaa actccggaag cttt                                   34


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to C)

<400>  21

Cys Ala Ser Arg Ser Arg Glu Thr Pro Glu Ala Phe 
1               5                   10          


<210>  22
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to G)

<400>  22
gtgcaagtcg ctggagggaa actccggaag cttt                                   34


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to G)

<400>  23

Cys Ala Ser Arg Trp Arg Glu Thr Pro Glu Ala Phe 
1               5                   10          


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to T)

<400>  24
gtgcaagtcg cttgagggaa actccggaag cttt                                   34


<210>  25
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR gene (mutate A to T)

<400>  25

Cys Ala Ser Arg Leu Arg Glu Thr Pro Glu Ala Phe 
1               5                   10          


<210>  26
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of donor TCR gene

<400>  26
tgtgccagct ctgagggagg ccaagacacc cagtac                                 36


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of donor TCR gene

<400>  27

Ser Glu Gly Gly Gln Asp 
1               5       


<210>  28
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of convergent V(D)J recombination

<400>  28
tgtgccagca gtcgacaggg tggtgaacag tac                                    33


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of convergent V(D)J recombination

<400>  29

Ser Arg Gln Gly Gly 
1               5   


<210>  30
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of recipient TCR gene

<400>  30
tgtgccagca gccgacaggg agggaaacag tac                                    33


<210>  31
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to G)

<400>  31
tgtgccagca gtgacgggac tgagtac                                           27


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of productive TCR gene found in peripheral blood

<400>  32
tgtgccagca ggggggaggt tgacacc                                           27


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of productive TCR gene found in peripheral blood

<400>  33

Cys Ala Ser Arg Gly Glu Val Asp Thr 
1               5                   


<210>  34
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR found in peripheral blood with 
       out-of-frame V and J gene segments

<400>  34
tgtgccaccg tatggaggga agtac                                             25


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete G)

<400>  35
tgtgccacct atggagggaa gtac                                              24


<210>  36
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete G)

<400>  36

Cys Ala Thr Tyr Gly Gly Lys Tyr 
1               5               


<210>  37
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete T)

<400>  37
tgtgccaccg atggagggaa gtac                                              24


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete T)

<400>  38

Cys Ala Thr Asp Gly Gly Lys Tyr 
1               5               


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete A)

<400>  39
tgtgccaccg ttggagggaa gtac                                              24


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete A or T)

<400>  40

Cys Ala Thr Val Gly Gly Lys Tyr 
1               5               


<210>  41
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete T)

<400>  41
tgtgccaccg taggagggaa gtac                                              24


<210>  42
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to G)

<400>  42

Cys Ala Ser Ser Asp Gly Thr Glu Tyr 
1               5                   


<210>  43
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete G)

<400>  43
tgtgccaccg tatggaggaa gtac                                              24


<210>  44
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (delete G)

<400>  44

Cys Ala Thr Val Trp Arg Glu Tyr 
1               5               


<210>  45
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR found in peripheral blood encoding 
       a stop codon in a somatic junction

<400>  45
tgtgccagca gtgacgggac ttagtac                                           27


<210>  46
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of non-productive TCR found in peripheral blood encoding 
       a stop codon in a somatic junction

<400>  46

Cys Ala Ser Ser Asp Gly Thr Tyr 
1               5               


<210>  47
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to A)

<400>  47
tgtgccagca gtgacgggac taagtac                                           27


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of repaired TCR found in peripheral blood (mutate T to A)

<400>  48

Cys Ala Ser Ser Asp Gly Thr Lys Tyr 
1               5                   


