                         SEQUENCE LISTING

<110>  CureImmune Therapeutics Inc.
 
<120>  BIFUNCTIONAL HOMODIMERIC ANTI-PD-1 AND IL-15/IL-15Ralpha FUSION 
       PROTEINS AND USES THEREOF

<130>  220096.402WO

<150>  US 63/158,801
<151>  2021-03-09

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VH-CDR1

<400>  1

Gly Phe Thr Phe Ser Ser Tyr Ala Met Ser 
1               5                   10  


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VH-CDR2

<400>  2

Ile Ser Gly Ser Gly Gly Ser Thr Tyr 
1               5                   


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VH-CDR3

<400>  3

Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr 
1               5                   10  


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VL-CDR1

<400>  4

Leu Ala Ile Ser Gly Trp 
1               5       


<210>  5
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VL-CDR2

<400>  5

Gly Ala Thr 
1           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VL-CDR3

<400>  6

Gln Gln Tyr Tyr Asp Tyr Pro Leu Thr 
1               5                   


<210>  7
<211>  1832
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-IL-15-Fc/co-sushi (BFP5)-Heavy chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  7
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctgattc acctttagca gctatgccat gagctgggtc cgccaggctc      180

cagggaaggg gctggagtgg gtctcagcta ttagtggtag tggtggtagc acatactacg      240

cagactccgt gaagggccgg ttcaccatct ccagagacaa ttccaagaac acgctgtatc      300

tgcaaatgaa cagcctgaga gccgaggaca cggccgtata ttactgtgcg agtcccgact      360

acggtgaatt gcgctactgg ggccagggaa ccctggtcac cgtctcctca gctagcacca      420

agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg ggcacagcgg      480

ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg tggaactcag      540

gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca ggactctact      600

ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc tacatctgca      660

acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagtcg ggcggtagcg      720

ggggtggagg aagtggaggt ggttccggag gcggtggttc gttgcagaac tgggtgaatg      780

taataagtga tttgaaaaaa attgaagatc ttattcaatc tatgcatatt gatgctactt      840

tatatacgga aagtgatgtt caccccagtt gcaaagtaac agcaatgaag tgctttctct      900

tggagttaca agttatttca cttgagtccg gagatgcaag tattcatgat acagtagaaa      960

atctgatcat cctagcaaac aacagtttgt cttctaatgg gaatgtaaca gaatctggat     1020

gcaaagaatg tgaggaactg gaggaaaaaa atattaaaga atttttgcag agttttgtac     1080

atattgtcca aatgttcatc aacacttctg gcggtggatc tattgaaggc cgcatggacc     1140

ccaaatcttg tgacaaaact cacacatgcc caccttgccc agcaccacct gtggcaggac     1200

cttcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg     1260

aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt     1320

acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca     1380

gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg     1440

agtacaagtg caaggtctcc aacaaaggcc tcccatcctc catcgagaaa accatctcca     1500

aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga     1560

tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg     1620

ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc     1680

tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc     1740

agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacgc     1800

agaagagcct ctccctgtct ccgggtaaat ga                                   1832


<210>  8
<211>  610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-IL-15-Fc/co-sushi (BFP5)-Heavy chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  8

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
            260                 265                 270         


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
        275                 280                 285             


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
    290                 295                 300                 


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
305                 310                 315                 320 


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
                325                 330                 335     


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
            340                 345                 350         


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
        355                 360                 365             


Thr Ser Gly Gly Gly Ser Ile Glu Gly Arg Met Asp Pro Lys Ser Cys 
    370                 375                 380                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly 
385                 390                 395                 400 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                405                 410                 415     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            420                 425                 430         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        435                 440                 445             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    450                 455                 460                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
465                 470                 475                 480 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
                485                 490                 495     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            500                 505                 510         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        515                 520                 525             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    530                 535                 540                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
545                 550                 555                 560 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                565                 570                 575     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            580                 585                 590         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        595                 600                 605             


Gly Lys 
    610 


<210>  9
<211>  2087
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-IL-15-sushi-Fc (BFP6)-Heavy Chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  9
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcact gttggagtct gggggaggct tggtacagcc tggggggtcc ctgagactct      120

cctgtgcagc ctctggattc acctttagca gctatgccat gagctgggtc cgccaggctc      180

cagggaaggg gctggagtgg gtctcagcta ttagtggtag tggtggtagc acatactacg      240

cagactccgt gaagggccgg ttcaccatct ccagagacaa ttccaagaac acgctgtatc      300

tgcaaatgaa cagcctgaga gccgaggaca cggccgtata ttactgtgcg agtcccgact      360

acggtgaatt gcgctactgg ggccagggaa ccctggtcac cgtctcctca gctagcacca      420

agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg ggcacagcgg      480

ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg tggaactcag      540

gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca ggactctact      600

ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc tacatctgca      660

acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagtcg ggcggtagcg      720

ggggtggagg aagtggaggt ggttccggag gcggtggttc gttgcagaac tgggtgaatg      780

taataagtga tttgaaaaaa attgaagatc ttattcaatc tatgcatatt gatgctactt      840

tatatacgga aagtgatgtt caccccagtt gcaaagtaac agcaatgaag tgctttctct      900

tggagttaca agttatttca cttgagtccg gagatgcaag tattcatgat acagtagaaa      960

atctgatcat cctagcaaac aacagtttgt cttctaatgg gaatgtaaca gaatctggat     1020

gcaaagaatg tgaggaactg gaggaaaaaa atattaaaga atttttgcag agttttgtac     1080

atattgtcca aatgttcatc aacacttcta gtggaggctc cggaggaggc ggttcgggtg     1140

ggggcagcgg tggtggaggg tccttgcaga tcacgtgccc tccccccatg tccgtggaac     1200

acgcagacat ctgggtcaag agctacagct tgtactccag ggagcggtac atttgtaact     1260

ctggtttcaa gcgtaaagcc ggcacgtcca gcctgacgga gtgcgtgttg aacaaggcca     1320

cgaatgtcgc ccactggaca acccccagtc tcaaatgcat taagggcggt ggatctattg     1380

aaggccgcat ggaccccaaa tcttgtgaca aaactcacac atgcccacct tgcccagcac     1440

cacctgtggc aggaccttca gtcttcctct tccccccaaa acccaaggac accctcatga     1500

tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg     1560

tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg     1620

aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact     1680

ggctgaatgg caaggagtac aagtgcaagg tctccaacaa aggcctccca tcctccatcg     1740

agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc     1800

catcccggga ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct     1860

atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga     1920

ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg     1980

acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat gaggctctgc     2040

acaaccacta cacgcagaag agcctctccc tgtctccggg taaatga                   2087


<210>  10
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-IL-15-sushi-Fc (BFP6)-Heavy Chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  10

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
            260                 265                 270         


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
        275                 280                 285             


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
    290                 295                 300                 


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
305                 310                 315                 320 


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
                325                 330                 335     


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
            340                 345                 350         


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
        355                 360                 365             


Thr Ser Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly 
    370                 375                 380                 


Gly Gly Gly Ser Leu Gln Ile Thr Cys Pro Pro Pro Met Ser Val Glu 
385                 390                 395                 400 


His Ala Asp Ile Trp Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg 
                405                 410                 415     


Tyr Ile Cys Asn Ser Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu 
            420                 425                 430         


Thr Glu Cys Val Leu Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr 
        435                 440                 445             


Pro Ser Leu Lys Cys Ile Lys Gly Gly Gly Ser Ile Glu Gly Arg Met 
    450                 455                 460                 


Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
465                 470                 475                 480 


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                485                 490                 495     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            500                 505                 510         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        515                 520                 525             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    530                 535                 540                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
545                 550                 555                 560 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
                565                 570                 575     


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            580                 585                 590         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        595                 600                 605             


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    610                 615                 620                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
625                 630                 635                 640 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                645                 650                 655     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            660                 665                 670         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        675                 680                 685             


Leu Ser Leu Ser Pro Gly Lys 
    690                 695 


<210>  11
<211>  99
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(99)
<223>  CH1

<400>  11

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu 
            


<210>  12
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(107)
<223>  CL

<400>  12

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  13
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VH

<400>  13

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  14
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  #61 VL

<400>  14

Glu Ile Val Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Leu Ala Ile Ser Gly Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Thr Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Asp Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  15
<211>  288
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(288)
<223>  human PD-1, NCBI Reference Sequence NP_005009

<400>  15

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  16
<211>  288
<212>  PRT
<213>  Macaca fascicularis


<220>
<221>  MISC_FEATURE
<222>  (1)..(288)
<223>  cynomologus monkey PD-1, Genbank Accession No. ABR15751

<400>  16

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Glu Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Ala Pro Thr Phe Ser Pro Ala Leu Leu Leu Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Ala Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Arg Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Ala Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Gln Gly Thr Ile Glu Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Ala Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Leu Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Pro Arg Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  17
<211>  14
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(14)
<223>  IgG1 hinge region

<400>  17

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  


<210>  18
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(107)
<223>  LC kappa constant domain

<400>  18

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  19
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fc region null mutant with E233P, L234V, L235A, delta236, A327G, 
       A330S, and P331S mutations

<400>  19

Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
1               5                   10                  15      


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
            20                  25                  30          


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
        35                  40                  45              


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
    50                  55                  60                  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
65                  70                  75                  80  


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
                85                  90                  95      


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
            100                 105                 110         


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
        115                 120                 125             


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
    130                 135                 140                 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
145                 150                 155                 160 


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
                165                 170                 175     


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
            180                 185                 190         


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
        195                 200                 205             


Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215     


<210>  20
<211>  114
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(114)
<223>  IL-15 polypeptide

<400>  20

Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
1               5                   10                  15      


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
            20                  25                  30          


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
        35                  40                  45              


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
    50                  55                  60                  


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
65                  70                  75                  80  


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
                85                  90                  95      


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
            100                 105                 110         


Thr Ser 
        


<210>  21
<211>  65
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(65)
<223>  IL-15Ralpha sushi domain

<400>  21

Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
1               5                   10                  15      


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
            20                  25                  30          


Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn 
        35                  40                  45              


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
    50                  55                  60                  


Lys 
65  


<210>  22
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified IL-15 polypeptide with L52C substitution

<400>  22

Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
1               5                   10                  15      


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
            20                  25                  30          


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
        35                  40                  45              


Val Ile Ser Cys Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
    50                  55                  60                  


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
65                  70                  75                  80  


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
                85                  90                  95      


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
            100                 105                 110         


Thr Ser 
        


<210>  23
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified IL-15Ralpha sushi domain with S40C substitution

<400>  23

Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
1               5                   10                  15      


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
            20                  25                  30          


Phe Lys Arg Lys Ala Gly Thr Cys Ser Leu Thr Glu Cys Val Leu Asn 
        35                  40                  45              


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
    50                  55                  60                  


Lys 
65  


<210>  24
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1st or 2nd linker or linker C or linker D

<400>  24

Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Leu Gln 
            20  


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3rd linker or linker E

<400>  25

Gly Gly Gly Ser Ile Glu Gly Arg Met Asp 
1               5                   10  


<210>  26
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi-IL-15-Fc (BFP1)-Heavy Chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  26
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgagag tggaggctcc      720

ggaggaggcg gttcgggtgg gggcagcggt ggtggagggt ccttgcagat cacgtgccct      780

ccccccatgt ccgtggaaca cgcagacatc tgggtcaaga gctacagctt gtactccagg      840

gagcggtaca tttgtaactc tggtttcaag cgtaaagccg gcacgtccag cctgacggag      900

tgcgtgttga acaaggccac gaatgtcgcc cactggacaa cccccagtct caaatgcatt      960

aagtcgggcg gtagcggggg tggaggaagt ggaggtggtt ccggaggcgg tggttcgttg     1020

cagaactggg tgaatgtaat aagtgatttg aaaaaaattg aagatcttat tcaatctatg     1080

catattgatg ctactttata tacggaaagt gatgttcacc ccagttgcaa agtaacagca     1140

atgaagtgct ttctcttgga gttacaagtt atttcacttg agtccggaga tgcaagtatt     1200

catgatacag tagaaaatct gatcatccta gcaaacaaca gtttgtcttc taatgggaat     1260

gtaacagaat ctggatgcaa agaatgtgag gaactggagg aaaaaaatat taaagaattt     1320

ttgcagagtt ttgtacatat tgtccaaatg ttcatcaaca cttctggcgg tggatctatt     1380

gaaggccgca tggaccccaa atcttgtgac aaaactcaca catgcccacc ttgcccagca     1440

ccacctgtgg caggaccttc agtcttcctc ttccccccaa aacccaagga caccctcatg     1500

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     1560

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     1620

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     1680

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aaggcctccc atcctccatc     1740

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     1800

ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     1860

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag     1920

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     1980

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     2040

cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatga                  2088


<210>  27
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi-IL-15-Fc (BFP1)-Heavy Chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  27

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
            260                 265                 270         


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
        275                 280                 285             


Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn 
    290                 295                 300                 


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
305                 310                 315                 320 


Lys Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Gly Ser Leu Gln Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
            340                 345                 350         


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
        355                 360                 365             


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
    370                 375                 380                 


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
385                 390                 395                 400 


His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser 
                405                 410                 415     


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
            420                 425                 430         


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
        435                 440                 445             


Gln Met Phe Ile Asn Thr Ser Gly Gly Gly Ser Ile Glu Gly Arg Met 
    450                 455                 460                 


Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
465                 470                 475                 480 


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                485                 490                 495     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            500                 505                 510         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        515                 520                 525             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    530                 535                 540                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
545                 550                 555                 560 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
                565                 570                 575     


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            580                 585                 590         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        595                 600                 605             


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    610                 615                 620                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
625                 630                 635                 640 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                645                 650                 655     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            660                 665                 670         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        675                 680                 685             


Leu Ser Leu Ser Pro Gly Lys 
    690                 695 


<210>  28
<211>  1686
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-sushi-Fc/co-IL-15 (BFP2)-Heavy chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  28
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgagag tggaggctcc      720

ggaggaggcg gttcgggtgg gggcagcggt ggtggagggt ccttgcagat cacgtgccct      780

ccccccatgt ccgtggaaca cgcagacatc tgggtcaaga gctacagctt gtactccagg      840

gagcggtaca tttgtaactc tggtttcaag cgtaaagccg gcacgtccag cctgacggag      900

tgcgtgttga acaaggccac gaatgtcgcc cactggacaa cccccagtct caaatgcatt      960

aagggcggtg gatctattga aggccgcatg gaccccaaat cttgtgacaa aactcacaca     1020

tgcccacctt gcccagcacc acctgtggca ggaccttcag tcttcctctt ccccccaaaa     1080

cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg     1140

agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat     1200

gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc     1260

accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa     1320

ggcctcccat cctccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca     1380

caggtgtaca ccctgccccc atcccgggag gagatgacca agaaccaggt cagcctgacc     1440

tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag     1500

ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc     1560

tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc     1620

gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt     1680

aaatga                                                                1686


<210>  29
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-sushi-Fc/co-IL-15 (BFP2)-Heavy chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  29

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
            260                 265                 270         


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
        275                 280                 285             


Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn 
    290                 295                 300                 


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
305                 310                 315                 320 


Lys Gly Gly Gly Ser Ile Glu Gly Arg Met Asp Pro Lys Ser Cys Asp 
                325                 330                 335     


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
            340                 345                 350         


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
        355                 360                 365             


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
    370                 375                 380                 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
385                 390                 395                 400 


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
                405                 410                 415     


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
            420                 425                 430         


Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys 
        435                 440                 445             


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
    450                 455                 460                 


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
465                 470                 475                 480 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
                485                 490                 495     


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
            500                 505                 510         


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
        515                 520                 525             


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
    530                 535                 540                 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
545                 550                 555                 560 


Lys 
    


<210>  30
<211>  1686
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi (S40C)-Fc/co-IL15 (L52C) (BFP3)-Heavy chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  30
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgagag tggaggctcc      720

ggaggaggcg gttcgggtgg gggcagcggt ggtggagggt ccttgcagat cacgtgccct      780

ccccccatgt ccgtggaaca cgcagacatc tgggtcaaga gctacagctt gtactccagg      840

gagcggtaca tttgtaactc tggtttcaag cgtaaagccg gcacgtgcag cctgacggag      900

tgcgtgttga acaaggccac gaatgtcgcc cactggacaa cccccagtct caaatgcatt      960

aagggcggtg gatctattga aggccgcatg gaccccaaat cttgtgacaa aactcacaca     1020

tgcccacctt gcccagcacc acctgtggca ggaccttcag tcttcctctt ccccccaaaa     1080

cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg     1140

agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat     1200

gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc     1260

accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa     1320

ggcctcccat cctccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca     1380

caggtgtaca ccctgccccc atcccgggag gagatgacca agaaccaggt cagcctgacc     1440

tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag     1500

ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc     1560

tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc     1620

gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt     1680

aaatga                                                                1686


<210>  31
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi (S40C)-Fc/co-IL15 (L52C) (BFP3)-Heavy chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  31

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
            260                 265                 270         


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
        275                 280                 285             


Phe Lys Arg Lys Ala Gly Thr Cys Ser Leu Thr Glu Cys Val Leu Asn 
    290                 295                 300                 


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
305                 310                 315                 320 


Lys Gly Gly Gly Ser Ile Glu Gly Arg Met Asp Pro Lys Ser Cys Asp 
                325                 330                 335     


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
            340                 345                 350         


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
        355                 360                 365             


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
    370                 375                 380                 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
385                 390                 395                 400 


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
                405                 410                 415     


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
            420                 425                 430         


Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys 
        435                 440                 445             


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
    450                 455                 460                 


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
465                 470                 475                 480 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
                485                 490                 495     


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
            500                 505                 510         


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
        515                 520                 525             


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
    530                 535                 540                 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
545                 550                 555                 560 


Lys 
    


<210>  32
<211>  1833
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-IL-15(L52C)-Fc/co-sushi (S40C) (BFP4)-Heavy Chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  32
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgagag tggaggctcc      720

ggaggaggcg gtagtggagg tggttccgga ggcggtggtt cgttgcagaa ctgggtgaat      780

gtaataagtg atttgaaaaa aattgaagat cttattcaat ctatgcatat tgatgctact      840

ttatatacgg aaagtgatgt tcaccccagt tgcaaagtaa cagcaatgaa gtgctttctc      900

ttggagttac aagttatttc atgtgagtcc ggagatgcaa gtattcatga tacagtagaa      960

aatctgatca tcctagcaaa caacagtttg tcttctaatg ggaatgtaac agaatctgga     1020

tgcaaagaat gtgaggaact ggaggaaaaa aatattaaag aatttttgca gagttttgta     1080

catattgtcc aaatgttcat caacacttct ggcggtggat ctattgaagg ccgcatggac     1140

cccaaatctt gtgacaaaac tcacacatgc ccaccttgcc cagcaccacc tgtggcagga     1200

ccttcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct     1260

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg     1320

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac     1380

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag     1440

gagtacaagt gcaaggtctc caacaaaggc ctcccatcct ccatcgagaa aaccatctcc     1500

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag     1560

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc     1620

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg     1680

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg     1740

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg     1800

cagaagagcc tctccctgtc tccgggtaaa tga                                  1833


<210>  33
<211>  610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-1Ab-IL-15(L52C)-Fc/co-sushi (S40C) (BFP4)-Heavy Chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  33

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
            260                 265                 270         


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
        275                 280                 285             


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
    290                 295                 300                 


Val Ile Ser Cys Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
305                 310                 315                 320 


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
                325                 330                 335     


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
            340                 345                 350         


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
        355                 360                 365             


Thr Ser Gly Gly Gly Ser Ile Glu Gly Arg Met Asp Pro Lys Ser Cys 
    370                 375                 380                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly 
385                 390                 395                 400 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                405                 410                 415     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            420                 425                 430         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        435                 440                 445             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    450                 455                 460                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
465                 470                 475                 480 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
                485                 490                 495     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            500                 505                 510         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        515                 520                 525             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    530                 535                 540                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
545                 550                 555                 560 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                565                 570                 575     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            580                 585                 590         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        595                 600                 605             


Gly Lys 
    610 


<210>  34
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-PD1 no. 61 light chain

<400>  34

Glu Ile Val Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Leu Ala Ile Ser Gly Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Thr Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Asp Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  35
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-PD1 no. 61 heavy chain Fab


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  35

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu 
225                 230                 235     


<210>  36
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-PD1 no. 61 heavy chain Fab


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  36
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgag                   708


<210>  37
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3rd linker or linker E between IL-15/or IL-15 (L52C)/or sushi/or 
       sushi (S40C) and Hinge

<400>  37
ggcggtggat ctattgaagg ccgcatggac                                        30


<210>  38
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1st or 2nd linker or linker C or linker D

<400>  38
agtggaggct ccggaggagg cggtagtgga ggtggttccg gaggcggtgg ttcgttgcag       60


<210>  39
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1st or 2nd linker or linker C or linker D

<400>  39
tcgggcggta gcgggggtgg aggaagtgga ggtggttccg gaggcggtgg ttcgttgcag       60


<210>  40
<211>  195
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(195)
<223>  IL-15Ralpha sushi domain

<400>  40
atcacgtgcc ctccccccat gtccgtggaa cacgcagaca tctgggtcaa gagctacagc       60

ttgtactcca gggagcggta catttgtaac tctggtttca agcgtaaagc cggcacgtcc      120

agcctgacgg agtgcgtgtt gaacaaggcc acgaatgtcg cccactggac aacccccagt      180

ctcaaatgca ttaag                                                       195


<210>  41
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified IL-15Ralpha sushi domain with S40C substitution

<400>  41
atcacgtgcc ctccccccat gtccgtggaa cacgcagaca tctgggtcaa gagctacagc       60

ttgtactcca gggagcggta catttgtaac tctggtttca agcgtaaagc cggcacgtgc      120

agcctgacgg agtgcgtgtt gaacaaggcc acgaatgtcg cccactggac aacccccagt      180

ctcaaatgca ttaag                                                       195


<210>  42
<211>  342
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(342)
<223>  IL-15

<400>  42
aactgggtga atgtaataag tgatttgaaa aaaattgaag atcttattca atctatgcat       60

attgatgcta ctttatatac ggaaagtgat gttcacccca gttgcaaagt aacagcaatg      120

aagtgctttc tcttggagtt acaagttatt tcacttgagt ccggagatgc aagtattcat      180

gatacagtag aaaatctgat catcctagca aacaacagtt tgtcttctaa tgggaatgta      240

acagaatctg gatgcaaaga atgtgaggaa ctggaggaaa aaaatattaa agaatttttg      300

cagagttttg tacatattgt ccaaatgttc atcaacactt ct                         342


<210>  43
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified IL-15 with L52C substitution

<400>  43
aactgggtga atgtaataag tgatttgaaa aaaattgaag atcttattca atctatgcat       60

attgatgcta ctttatatac ggaaagtgat gttcacccca gttgcaaagt aacagcaatg      120

aagtgctttc tcttggagtt acaagttatt tcatgtgagt ccggagatgc aagtattcat      180

gatacagtag aaaatctgat catcctagca aacaacagtt tgtcttctaa tgggaatgta      240

acagaatctg gatgcaaaga atgtgaggaa ctggaggaaa aaaatattaa agaatttttg      300

cagagttttg tacatattgt ccaaatgttc atcaacactt ct                         342


<210>  44
<211>  651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fc region null mutant with E233P, L234V, L235A, delta236, A327G, 
       A330S, and P331S

<400>  44
gcaccacctg tggcaggacc ttcagtcttc ctcttccccc caaaacccaa ggacaccctc       60

atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct      120

gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg      180

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag      240

gactggctga atggcaagga gtacaagtgc aaggtctcca acaaaggcct cccatcctcc      300

atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg      360

cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc      420

ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac      480

aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc      540

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct      600

ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaatg a               651


<210>  45
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-PD1 no. 61 light chain

<400>  45
gaaattgtaa tgacacagtc tccatcttct gtgtctgcat ctgtaggaga cagagtcacc       60

atcacttgtc gggcgagtct ggctattagc ggctggttag cctggtatca gcagacacca      120

gggaaagccc ctaggctcct gatctatggt gcaaccacgt tgcagagtgg ggttccatca      180

cggttcagcg gcagtggatc tgggacagat ttcactctca ccatcagccg cctgcagtct      240

gaagattttg caacttatta ctgtcaacaa tattatgatt accctctcac tttcggcgga      300

ggaaccaagg tggagatcaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      480

gagagtgtca cagagcaaga cagcaaggac agcacctaca gcctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gt                         642


<210>  46
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alternative 1st or 2nd linker

<400>  46

Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Leu Gln 
            20                  25      


<210>  47
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1st or 2nd linker

<400>  47
agtggaggct ccggaggagg cggttcgggt gggggcagcg gtggtggagg gtccttgcag       60


<210>  48

<400>  48
000

<210>  49

<400>  49
000

<210>  50

<400>  50
000

<210>  51

<400>  51
000

<210>  52
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-15 (I67E) polypeptide

<400>  52

Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
1               5                   10                  15      


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
            20                  25                  30          


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
        35                  40                  45              


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
    50                  55                  60                  


Asn Leu Glu Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
65                  70                  75                  80  


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
                85                  90                  95      


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
            100                 105                 110         


Thr Ser 
        


<210>  53
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-15 (I67E)

<400>  53
aactgggtga atgtaataag tgatttgaaa aaaattgaag atcttattca atctatgcat       60

attgatgcta ctttatatac ggaaagtgat gttcacccca gttgcaaagt aacagcaatg      120

aagtgctttc tcttggagtt acaagttatt tcacttgagt ccggagatgc aagtattcat      180

gatacagtag aaaatctgga aatcctagca aacaacagtt tgtcttctaa tgggaatgta      240

acagaatctg gatgcaaaga atgtgaggaa ctggaggaaa aaaatattaa agaatttttg      300

cagagttttg tacatattgt ccaaatgttc atcaacactt ct                         342


<210>  54
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi-IL-15 (I67E)-Fc (BFP11) heavy chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal peptide

<400>  54

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ser Pro Asp Tyr Gly Glu Leu Arg Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Ser Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Gln 
                245                 250                 255     


Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
            260                 265                 270         


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
        275                 280                 285             


Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn 
    290                 295                 300                 


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
305                 310                 315                 320 


Lys Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly 
                325                 330                 335     


Gly Gly Ser Leu Gln Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
            340                 345                 350         


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
        355                 360                 365             


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
    370                 375                 380                 


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
385                 390                 395                 400 


His Asp Thr Val Glu Asn Leu Glu Ile Leu Ala Asn Asn Ser Leu Ser 
                405                 410                 415     


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
            420                 425                 430         


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
        435                 440                 445             


Gln Met Phe Ile Asn Thr Ser Gly Gly Gly Ser Ile Glu Gly Arg Met 
    450                 455                 460                 


Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
465                 470                 475                 480 


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                485                 490                 495     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            500                 505                 510         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        515                 520                 525             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    530                 535                 540                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
545                 550                 555                 560 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
                565                 570                 575     


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            580                 585                 590         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        595                 600                 605             


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    610                 615                 620                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
625                 630                 635                 640 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                645                 650                 655     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            660                 665                 670         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        675                 680                 685             


Leu Ser Leu Ser Pro Gly Lys 
    690                 695 


<210>  55
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1 Ab-sushi-IL-15 (I67E)-Fc (BFP11) heavy chain


<220>
<221>  misc_signal
<222>  (1)..(60)
<223>  encoding signal peptide

<400>  55
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggc       60

gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc      120

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      180

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      240

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      300

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagtcccgac      360

tacggtgaat tgcgctactg gggccaggga accctggtca ccgtctcctc agctagcacc      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga aagttgagag tggaggctcc      720

ggaggaggcg gttcgggtgg gggcagcggt ggtggagggt ccttgcagat cacgtgccct      780

ccccccatgt ccgtggaaca cgcagacatc tgggtcaaga gctacagctt gtactccagg      840

gagcggtaca tttgtaactc tggtttcaag cgtaaagccg gcacgtccag cctgacggag      900

tgcgtgttga acaaggccac gaatgtcgcc cactggacaa cccccagtct caaatgcatt      960

aagtcgggcg gtagcggggg tggaggaagt ggaggtggtt ccggaggcgg tggttcgttg     1020

cagaactggg tgaatgtaat aagtgatttg aaaaaaattg aagatcttat tcaatctatg     1080

catattgatg ctactttata tacggaaagt gatgttcacc ccagttgcaa agtaacagca     1140

atgaagtgct ttctcttgga gttacaagtt atttcacttg agtccggaga tgcaagtatt     1200

catgatacag tagaaaatct ggaaatccta gcaaacaaca gtttgtcttc taatgggaat     1260

gtaacagaat ctggatgcaa agaatgtgag gaactggagg aaaaaaatat taaagaattt     1320

ttgcagagtt ttgtacatat tgtccaaatg ttcatcaaca cttctggcgg tggatctatt     1380

gaaggccgca tggaccccaa atcttgtgac aaaactcaca catgcccacc ttgcccagca     1440

ccacctgtgg caggaccttc agtcttcctc ttccccccaa aacccaagga caccctcatg     1500

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     1560

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     1620

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     1680

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aaggcctccc atcctccatc     1740

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     1800

ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     1860

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag     1920

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     1980

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     2040

cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatga                  2088


<210>  56
<211>  15
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(15)
<223>  IgG1 reference hinge

<400>  56

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


