                         SEQUENCE LISTING

<110>  The Trustees Of The University Of Pennsylvania
       June, Carl H
       Young, Regina M
       Berger, Shelley L
       Good, Charly R
       Aznar Gomez, M. Angela
       Kuramitsu, Shunichiro
 
<120>  COMPOSITIONS AND METHODS FOR ASSESSING AND TREATING T CELL 
       DYSFUNCTION

<130>  046483-7314WO1(02832)

<150>  63/158,313
<151>  2021-03-08

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1
gctggtgcaa gaccccgagt                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  2
caagatccct ttcattcgag                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  3
tggtgtggtc gcagatcgag                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  4
aggaggcgat tcccagctcg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  5
cgagaacacg gaagcgctgc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  6
tggctaagct gagtgcctct                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  7
atgtcgtcca gcaagctcag                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  8
tgtagtcgat gacgcgctgt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  9
tggccagact gcgttccgac                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  10
ggtgcgcggc tgctacgagg                                                   20


<210>  11
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human anti-MSLN CAR

<400>  11

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Glu Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asp Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Trp Ile Asn Pro Asn Ser Gly Gly Thr Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Ser Gly Trp Asp Phe Asp Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
145                 150                 155                 160 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
                165                 170                 175     


Ile Thr Cys Arg Ala Ser Gln Ser Ile Arg Tyr Tyr Leu Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr Ala Ser 
        195                 200                 205             


Ile Leu Gln Asn Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
225                 230                 235                 240 


Thr Tyr Tyr Cys Leu Gln Thr Tyr Thr Thr Pro Asp Phe Gly Pro Gly 
                245                 250                 255     


Thr Lys Val Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
            260                 265                 270         


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
        275                 280                 285             


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
    290                 295                 300                 


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
305                 310                 315                 320 


Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys 
                325                 330                 335     


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
            340                 345                 350         


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
        355                 360                 365             


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
    370                 375                 380                 


Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
385                 390                 395                 400 


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
                405                 410                 415     


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
            420                 425                 430         


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
        435                 440                 445             


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
    450                 455                 460                 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
465                 470                 475                 480 


Ala Leu Pro Pro Arg 
                485 


<210>  12
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 Leader

<400>  12

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  13
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M5 scFv

<400>  13

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Trp Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser 
    130                 135                 140                 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Ser Ile Arg Tyr Tyr Leu Ser Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Lys Ala Pro Lys Leu Leu Ile Tyr Thr Ala Ser Ile Leu Gln Asn Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu 
    210                 215                 220                 


Gln Thr Tyr Thr Thr Pro Asp Phe Gly Pro Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 


Lys 
    


<210>  14
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  14

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  15
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  15

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  16
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB ICD

<400>  16

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  17
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta

<400>  17

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  18
<211>  1455
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human anti-MSLN CAR

<400>  18
atggccctcc ctgtcaccgc cctgctgctt ccgctggctc ttctgctcca cgccgctcgg       60

ccccaagtcc aactcgttca atcaggcgca gaagtcgaaa agcccggagc atcagtcaaa      120

gtctcttgca aggcttccgg ctacaccttc acggactact acatgcactg ggtgcgccag      180

gctccaggcc agggactgga gtggatggga tggatcaacc cgaattccgg gggaactaac      240

tacgcccaga agtttcaggg ccgggtgact atgactcgcg atacctcgat ctcgactgcg      300

tacatggagc tcagccgcct ccggtcggac gataccgccg tgtactattg tgcgtcggga      360

tgggacttcg actactgggg gcagggcact ctggtcactg tgtcaagcgg aggaggtgga      420

tcaggtggag gtggaagcgg gggaggaggt tccggcggcg gaggatcaga tatcgtgatg      480

acgcaatcgc cttcctcgtt gtccgcatcc gtgggagaca gggtgaccat tacttgcaga      540

gcgtcccagt ccattcggta ctacctgtcg tggtaccagc agaagccggg gaaagcccca      600

aaactgctta tctatactgc ctcgatcctc caaaacggcg tgccatcaag attcagcggt      660

tcgggcagcg ggaccgactt taccctgact atcagcagcc tgcagccgga agatttcgcc      720

acgtactact gcctgcaaac ctacaccacc ccggacttcg gacctggaac caaggtggag      780

atcaagacca ctaccccagc accgaggcca cccaccccgg ctcctaccat cgcctcccag      840

cctctgtccc tgcgtccgga ggcatgtaga cccgcagctg gtggggccgt gcatacccgg      900

ggtcttgact tcgcctgcga tatctacatt tgggcccctc tggctggtac ttgcggggtc      960

ctgctgcttt cactcgtgat cactctttac tgtaagcgcg gtcggaagaa gctgctgtac     1020

atctttaagc aacccttcat gaggcctgtg cagactactc aagaggagga cggctgttca     1080

tgccggttcc cagaggagga ggaaggcggc tgcgaactgc gcgtgaaatt cagccgcagc     1140

gcagatgctc cagcctacaa gcaggggcag aaccagctct acaacgaact caatcttggt     1200

cggagagagg agtacgacgt gctggacaag cggagaggac gggacccaga aatgggcggg     1260

aagccgcgca gaaagaatcc ccaagagggc ctgtacaacg agctccaaaa ggataagatg     1320

gcagaagcct atagcgagat tggtatgaaa ggggaacgca gaagaggcaa aggccacgac     1380

ggactgtacc agggactcag caccgccacc aaggacacct atgacgctct tcacatgcag     1440

gccctgccgc ctcgg                                                      1455


<210>  19
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 Leader

<400>  19
atggccctcc ctgtcaccgc cctgctgctt ccgctggctc ttctgctcca cgccgctcgg       60

ccc                                                                     63


<210>  20
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M5 scFv

<400>  20
caagtccaac tcgttcaatc aggcgcagaa gtcgaaaagc ccggagcatc agtcaaagtc       60

tcttgcaagg cttccggcta caccttcacg gactactaca tgcactgggt gcgccaggct      120

ccaggccagg gactggagtg gatgggatgg atcaacccga attccggggg aactaactac      180

gcccagaagt ttcagggccg ggtgactatg actcgcgata cctcgatctc gactgcgtac      240

atggagctca gccgcctccg gtcggacgat accgccgtgt actattgtgc gtcgggatgg      300

gacttcgact actgggggca gggcactctg gtcactgtgt caagcggagg aggtggatca      360

ggtggaggtg gaagcggggg aggaggttcc ggcggcggag gatcagatat cgtgatgacg      420

caatcgcctt cctcgttgtc cgcatccgtg ggagacaggg tgaccattac ttgcagagcg      480

tcccagtcca ttcggtacta cctgtcgtgg taccagcaga agccggggaa agccccaaaa      540

ctgcttatct atactgcctc gatcctccaa aacggcgtgc catcaagatt cagcggttcg      600

ggcagcggga ccgactttac cctgactatc agcagcctgc agccggaaga tttcgccacg      660

tactactgcc tgcaaaccta caccaccccg gacttcggac ctggaaccaa ggtggagatc      720

aag                                                                    723


<210>  21
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  21
accactaccc cagcaccgag gccacccacc ccggctccta ccatcgcctc ccagcctctg       60

tccctgcgtc cggaggcatg tagacccgca gctggtgggg ccgtgcatac ccggggtctt      120

gacttcgcct gcgat                                                       135


<210>  22
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  22
atctacattt gggcccctct ggctggtact tgcggggtcc tgctgctttc actcgtgatc       60

actctttact gt                                                           72


<210>  23
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4-1BB ICD

<400>  23
aagcgcggtc ggaagaagct gctgtacatc tttaagcaac ccttcatgag gcctgtgcag       60

actactcaag aggaggacgg ctgttcatgc cggttcccag aggaggagga aggcggctgc      120

gaactg                                                                 126


<210>  24
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 zeta

<400>  24
cgcgtgaaat tcagccgcag cgcagatgct ccagcctaca agcaggggca gaaccagctc       60

tacaacgaac tcaatcttgg tcggagagag gagtacgacg tgctggacaa gcggagagga      120

cgggacccag aaatgggcgg gaagccgcgc agaaagaatc cccaagaggg cctgtacaac      180

gagctccaaa aggataagat ggcagaagcc tatagcgaga ttggtatgaa aggggaacgc      240

agaagaggca aaggccacga cggactgtac cagggactca gcaccgccac caaggacacc      300

tatgacgctc ttcacatgca ggccctgccg cctcgg                                336


<210>  25
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human anti-CD19 CAR

<400>  25

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu 
            20                  25                  30          


Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala 
    50                  55                  60                  


Pro Arg Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Ile Pro 
65                  70                  75                  80  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln 
    130                 135                 140                 


Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr 
145                 150                 155                 160 


Leu Ser Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Gln Ser Ser Leu Lys Ser 
        195                 200                 205             


Arg Val Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val Ser Leu Lys 
    210                 215                 220                 


Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
305                 310                 315                 320 


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
                325                 330                 335     


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
            340                 345                 350         


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
        355                 360                 365             


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  26
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCD19 scFv

<400>  26

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Gln Ser Ser Leu Lys Ser Arg Val Thr Ile Ser 
            180                 185                 190         


Lys Asp Asn Ser Lys Asn Gln Val Ser Leu Lys Leu Ser Ser Val Thr 
        195                 200                 205             


Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  27
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  27

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  28
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 ICD

<400>  28

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  29
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta

<400>  29

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  30
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human anti-CD19 CAR

<400>  30
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggaaattg tgatgaccca gtcacccgcc actcttagcc tttcacccgg tgagcgcgca      120

accctgtctt gcagagcctc ccaagacatc tcaaaatacc ttaattggta tcaacagaag      180

cccggacagg ctcctcgcct tctgatctac cacaccagcc ggctccattc tggaatccct      240

gccaggttca gcggtagcgg atctgggacc gactacaccc tcactatcag ctcactgcag      300

ccagaggact tcgctgtcta tttctgtcag caagggaaca ccctgcccta cacctttgga      360

cagggcacca agctcgagat taaaggtgga ggtggcagcg gaggaggtgg gtccggcggt      420

ggaggaagcc aggtccaact ccaagaaagc ggaccgggtc ttgtgaagcc atcagaaact      480

ctttcactga cttgtactgt gagcggagtg tctctccccg attacggggt gtcttggatc      540

agacagccac cggggaaggg tctggaatgg attggagtga tttggggctc tgagactact      600

tactaccaat catccctcaa gtcacgcgtc accatctcaa aggacaactc taagaatcag      660

gtgtcactga aactgtcatc tgtgaccgca gccgacaccg ccgtgtacta ttgcgctaag      720

cattactatt atggcgggag ctacgcaatg gattactggg gacagggtac tctggtcacc      780

gtgtccagca ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatttttgg gtgctggtgg tggttggtgg agtcctggct      960

tgctatagct tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc     1020

aggctcctgc acagtgacta catgaacatg actccccgcc gccccgggcc cacccgcaag     1080

cattaccagc cctatgcccc accacgcgac ttcgcagcct atcgctccag agtgaagttc     1140

agcaggagcg cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc     1200

aatctaggac gaagagagga gtacgatgtt ttggacaaga gacgtggccg ggaccctgag     1260

atggggggaa agccgagaag gaagaaccct caggaaggcc tgtacaatga actgcagaaa     1320

gataagatgg cggaggccta cagtgagatt gggatgaaag gcgagcgccg gaggggcaag     1380

gggcacgatg gcctttacca gggtctcagt acagccacca aggacaccta cgacgccctt     1440

cacatgcagg ccctgccccc tcgc                                            1464


<210>  31
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 leader

<400>  31
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210>  32
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCD19 scFv

<400>  32
gaaattgtga tgacccagtc acccgccact cttagccttt cacccggtga gcgcgcaacc       60

ctgtcttgca gagcctccca agacatctca aaatacctta attggtatca acagaagccc      120

ggacaggctc ctcgccttct gatctaccac accagccggc tccattctgg aatccctgcc      180

aggttcagcg gtagcggatc tgggaccgac tacaccctca ctatcagctc actgcagcca      240

gaggacttcg ctgtctattt ctgtcagcaa gggaacaccc tgccctacac ctttggacag      300

ggcaccaagc tcgagattaa aggtggaggt ggcagcggag gaggtgggtc cggcggtgga      360

ggaagccagg tccaactcca agaaagcgga ccgggtcttg tgaagccatc agaaactctt      420

tcactgactt gtactgtgag cggagtgtct ctccccgatt acggggtgtc ttggatcaga      480

cagccaccgg ggaagggtct ggaatggatt ggagtgattt ggggctctga gactacttac      540

taccaatcat ccctcaagtc acgcgtcacc atctcaaagg acaactctaa gaatcaggtg      600

tcactgaaac tgtcatctgt gaccgcagcc gacaccgccg tgtactattg cgctaagcat      660

tactattatg gcgggagcta cgcaatggat tactggggac agggtactct ggtcaccgtg      720

tccagc                                                                 726


<210>  33
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  33
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  34
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  34
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt g                                                 81


<210>  35
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 ICD

<400>  35
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  36
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 zeta

<400>  36
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  37
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human anti-CD19 CAR

<400>  37

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu 
            20                  25                  30          


Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala 
    50                  55                  60                  


Pro Arg Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Ile Pro 
65                  70                  75                  80  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln 
    130                 135                 140                 


Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr 
145                 150                 155                 160 


Leu Ser Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Gln Ser Ser Leu Lys Ser 
        195                 200                 205             


Arg Val Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val Ser Leu Lys 
    210                 215                 220                 


Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  38
<211>  1458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human anti-CD19 CAR

<400>  38
atggccctcc ctgtcaccgc cctgctgctt ccgctggctc ttctgctcca cgccgctcgg       60

cccgaaattg tgatgaccca gtcacccgcc actcttagcc tttcacccgg tgagcgcgca      120

accctgtctt gcagagcctc ccaagacatc tcaaaatacc ttaattggta tcaacagaag      180

cccggacagg ctcctcgcct tctgatctac cacaccagcc ggctccattc tggaatccct      240

gccaggttca gcggtagcgg atctgggacc gactacaccc tcactatcag ctcactgcag      300

ccagaggact tcgctgtcta tttctgtcag caagggaaca ccctgcccta cacctttgga      360

cagggcacca agctcgagat taaaggtgga ggtggcagcg gaggaggtgg gtccggcggt      420

ggaggaagcc aggtccaact ccaagaaagc ggaccgggtc ttgtgaagcc atcagaaact      480

ctttcactga cttgtactgt gagcggagtg tctctccccg attacggggt gtcttggatc      540

agacagccac cggggaaggg tctggaatgg attggagtga tttggggctc tgagactact      600

tactaccaat catccctcaa gtcacgcgtc accatctcaa aggacaactc taagaatcag      660

gtgtcactga aactgtcatc tgtgaccgca gccgacaccg ccgtgtacta ttgcgctaag      720

cattactatt atggcgggag ctacgcaatg gattactggg gacagggtac tctggtcacc      780

gtgtccagca ccactacccc agcaccgagg ccacccaccc cggctcctac catcgcctcc      840

cagcctctgt ccctgcgtcc ggaggcatgt agacccgcag ctggtggggc cgtgcatacc      900

cggggtcttg acttcgcctg cgatatctac atttgggccc ctctggctgg tacttgcggg      960

gtcctgctgc tttcactcgt gatcactctt tactgtaagc gcggtcggaa gaagctgctg     1020

tacatcttta agcaaccctt catgaggcct gtgcagacta ctcaagagga ggacggctgt     1080

tcatgccggt tcccagagga ggaggaaggc ggctgcgaac tgcgcgtgaa attcagccgc     1140

agcgcagatg ctccagccta caagcagggg cagaaccagc tctacaacga actcaatctt     1200

ggtcggagag aggagtacga cgtgctggac aagcggagag gacgggaccc agaaatgggc     1260

gggaagccgc gcagaaagaa tccccaagag ggcctgtaca acgagctcca aaaggataag     1320

atggcagaag cctatagcga gattggtatg aaaggggaac gcagaagagg caaaggccac     1380

gacggactgt accagggact cagcaccgcc accaaggaca cctatgacgc tcttcacatg     1440

caggccctgc cgcctcgg                                                   1458


<210>  39
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-GD2 scFv

<400>  39

Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
1               5                   10                  15      


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
            20                  25                  30          


His Arg Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
        35                  40                  45              


Gln Ser Pro Lys Leu Leu Ile His Lys Val Ser Asn Arg Phe Ser Gly 
    50                  55                  60                  


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
65                  70                  75                  80  


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
                85                  90                  95      


Gln Ser Thr His Val Pro Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
            100                 105                 110         


Glu Leu Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Gln Ser Gly Pro 
    130                 135                 140                 


Glu Leu Glu Lys Pro Ser Ala Ser Val Met Ile Ser Cys Lys Ala Ser 
145                 150                 155                 160 


Gly Ser Ser Phe Thr Gly Tyr Asn Met Asn Trp Val Arg Gln Asn Ile 
                165                 170                 175     


Gly Lys Ser Leu Glu Trp Ile Gly Ala Ile Asp Pro Tyr Tyr Gly Gly 
            180                 185                 190         


Thr Ser Tyr Asn Gln Lys Phe Lys Gly Arg Ala Thr Leu Thr Val Asp 
        195                 200                 205             


Lys Ser Ser Ser Thr Ala Tyr Met His Leu Lys Ser Leu Thr Ser Glu 
    210                 215                 220                 


Asp Ser Val Tyr Tyr Cys Val Ser Gly Met Glu Tyr Trp Gly Gln Gly 
225                 230                 235                 240 


Thr Ser Val Thr Val Ser Ser Ser Gly 
                245                 


<210>  40
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-GD2 scFv

<400>  40
ggatccgatg ttgtgatgac ccaaactcca ctctccctgc ctgtcagtct tggagatcaa       60

gcctccatct cttgcagatc tagtcagagt cttgtacacc gtaacggaaa cacctattta      120

cattggtacc tgcagaagcc aggccagtct ccaaagctcc tgattcacaa agtttccaac      180

cgattttctg gggtcccaga caggttcagt ggcagtggat cagggacaga tttcacactc      240

aagatcagca gagtggaggc tgaggatctg ggagtttatt tctgttctca aagtacacac      300

gttcctccgc tcacgttcgg tgctgggacc aagctggagc tgaaaggagg tggcgggtca      360

gggggtggcg gaagcggagg cggcggttca ggcggaggag gctcggaggt gcagcttctg      420

cagtctggac ctgagctgga gaagccttcc gcttcagtga tgatatcctg caaggcttct      480

ggttcctcct tcactggcta caacatgaac tgggtgaggc agaatattgg aaagagcctt      540

gaatggattg gagctattga tccttactac ggtggaacta gctacaacca gaagttcaag      600

ggcagggcca cattgactgt agacaaatcg tccagcacag cctacatgca cctcaagagc      660

ctgacatctg aggactctgt ctattactgt gtaagcggaa tggagtactg gggtcaagga      720

acctcagtca ccgtctcctc atccgga                                          747


<210>  41
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-HER2 scFv

<400>  41

Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr 
            20                  25                  30          


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro 
                85                  90                  95      


Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Gly Ser 
            100                 105                 110         


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Glu Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Ile His 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile 
                165                 170                 175     


Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp 
    210                 215                 220                 


Gly Gly Asp Gly Phe Tyr Ala Met Asp Val Trp Gly Gln Gly Thr Leu 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  42
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-HER2 scFv

<400>  42
ggagatatcc agatgaccca gtccccgagc tccctgtccg cctctgtggg cgatagggtc       60

accatcacct gccgtgccag tcaggatgtg aatactgctg tagcctggta tcaacagaaa      120

ccaggaaaag ctccgaaact actgatttac tcggcatcct tcctttattc tggagtccct      180

tctcgcttct ctggatctag atctgggacg gatttcactc tgaccatcag cagtctgcag      240

ccggaagact tcgcaactta ttactgtcag caacattata ctactcctcc cacgttcgga      300

cagggtacca aggtggagat caaacgcact gggtctacat ctggatctgg gaagccgggt      360

tctggtgagg gttctgaggt tcagctggtg gagtctggcg gtggcctggt gcagccaggg      420

ggctcactcc gtttgtcctg tgcagcttct ggcttcaaca ttaaagacac ctatatacac      480

tgggtgcgtc aggccccggg taagggcctg gaatgggttg caaggattta tcctacgaat      540

ggttatacta gatatgccga tagcgtcaag ggccgtttca ctataagcgc agacacatcc      600

aaaaacacag cctacctgca gatgaacagc ctgcgtgctg aggacactgc cgtctattat      660

tgttctagat ggggagggga cggcttctat gctatggacg tgtggggtca aggaaccctg      720

gtcaccgtct cctcg                                                       735


<210>  43
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-HER2 scFv

<400>  43

Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr 
            20                  25                  30          


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Ser Ala Ser Phe Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro 
                85                  90                  95      


Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Gly Ser 
            100                 105                 110         


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Glu Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Ile His 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile 
                165                 170                 175     


Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp 
    210                 215                 220                 


Gly Gly Asp Gly Phe Val Ala Met Asp Val Trp Gly Gln Gly Thr Leu 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  44
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-HER2 scFv

<400>  44
ggagatatcc agatgaccca gtccccgagc tccctgtccg cctctgtggg cgatagggtc       60

accatcacct gccgtgccag tcaggatgtg aatactgctg tagcctggta tcaacagaaa      120

ccaggaaaag ctccgaaact actgatttac tcggcatcct tccttgagtc tggagtccct      180

tctcgcttct ctggatctag atctgggacg gatttcactc tgaccatcag cagtctgcag      240

ccggaagact tcgcaactta ttactgtcag caacattata ctactcctcc cacgttcgga      300

cagggtacca aggtggagat caaacgcact gggtctacat ctggatctgg gaagccgggt      360

tctggtgagg gttctgaggt tcagctggtg gagtctggcg gtggcctggt gcagccaggg      420

ggctcactcc gtttgtcctg tgcagcttct ggcttcaaca ttaaagacac ctatatacac      480

tgggtgcgtc aggccccggg taagggcctg gaatgggttg caaggattta tcctacgaat      540

ggttatacta gatatgccga tagcgtcaag ggccgtttca ctataagcgc agacacatcc      600

aaaaacacag cctacctgca gatgaacagc ctgcgtgctg aggacactgc cgtctattat      660

tgttctagat ggggagggga cggcttcgtt gctatggacg tgtggggtca aggaaccctg      720

gtcaccgtct cctcg                                                       735


<210>  45
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-TnMuc1 scFv

<400>  45

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr Asn Asp Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Glu Leu Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val 
    130                 135                 140                 


Thr Ala Gly Glu Lys Val Thr Met Ile Cys Lys Ser Ser Gln Ser Leu 
145                 150                 155                 160 


Leu Asn Ser Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Pro Pro Lys Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Gln Asn Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Leu Lys 
                


<210>  46
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-TnMuc1 scFv

<400>  46
caggtgcagc tgcagcagtc tgatgccgag ctcgtgaagc ctggcagcag cgtgaagatc       60

agctgcaagg ccagcggcta caccttcacc gaccacgcca tccactgggt caagcagaag      120

cctgagcagg gcctggagtg gatcggccac ttcagccccg gcaacaccga catcaagtac      180

aacgacaagt tcaagggcaa ggccaccctg accgtggaca gaagcagcag caccgcctac      240

atgcagctga acagcctgac cagcgaggac agcgccgtgt acttctgcaa gaccagcacc      300

ttctttttcg actactgggg ccagggcaca accctgacag tgtctagcgg aggcggagga      360

tctggcggcg gaggaagtgg cggaggggga tctgaactcg tgatgaccca gagccccagc      420

tctctgacag tgacagccgg cgagaaagtg accatgatct gcaagtcctc ccagagcctg      480

ctgaactccg gcgaccagaa gaactacctg acctggtatc agcagaaacc cggccagccc      540

cccaagctgc tgatcttttg ggccagcacc cgggaaagcg gcgtgcccga tagattcaca      600

ggcagcggct ccggcaccga ctttaccctg accatcagct ccgtgcaggc cgaggacctg      660

gccgtgtatt actgccagaa cgactacagc taccccctga ccttcggagc cggcaccaag      720

ctggaactga ag                                                          732


<210>  47
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PSMA scFv

<400>  47

Gly Ser Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp Val Gly 
            20                  25                  30          


Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe 
    50                  55                  60                  


Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Asn Val 
65                  70                  75                  80  


Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr 
                85                  90                  95      


Pro Leu Thr Phe Gly Ala Gly Thr Met Leu Asp Leu Lys Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser Glu Val Gln Leu 
        115                 120                 125             


Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Thr Ser Val Arg Ile 
    130                 135                 140                 


Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Glu Tyr Thr Ile His Trp 
145                 150                 155                 160 


Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile Gly Asn Ile Asn 
                165                 170                 175     


Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe Glu Asp Lys Ala 
            180                 185                 190         


Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Arg 
        195                 200                 205             


Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Ala Gly Trp 
    210                 215                 220                 


Asn Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala 
225                 230                 235                 240 


Ser Ser Gly 
            


<210>  48
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-PSMA scFv

<400>  48
ggatctgaca ttgtgatgac ccagtctcac aaattcatgt ccacatcagt aggagacagg       60

gtcagcatca tctgtaaggc cagtcaagat gtgggtactg ctgtagactg gtatcaacag      120

aaaccaggac aatctcctaa actactgatt tattgggcat ccactcggca cactggagtc      180

cctgatcgct tcacaggcag tggatctggg acagacttca ctctcaccat tactaacgtt      240

cagtctgaag acttggcaga ttatttctgt cagcaatata acagctatcc tctcacgttc      300

ggtgctggga ccatgctgga cctgaaagga ggcggaggat ctggcggcgg aggaagttct      360

ggcggaggca gcgaggtgca gctgcagcag agcggacccg agctcgtgaa gcctggaaca      420

agcgtgcgga tcagctgcaa gaccagcggc tacaccttca ccgagtacac catccactgg      480

gtcaagcagt cccacggcaa gagcctggag tggatcggca atatcaaccc caacaacggc      540

ggcaccacct acaaccagaa gttcgaggac aaggccaccc tgaccgtgga caagagcagc      600

agcaccgcct acatggaact gcggagcctg accagcgagg acagcgccgt gtactattgt      660

gccgccggtt ggaacttcga ctactggggc cagggcacaa ccctgacagt gtctagcgct      720

agctccgga                                                              729


<210>  49
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-EGFRvIII scFv

<400>  49

Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn Ile Glu Asp Tyr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr Gly Pro Ile Phe 
    50                  55                  60                  


Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr Thr Val Thr Val 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Asp Ser 
    130                 135                 140                 


Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 
145                 150                 155                 160 


Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Gln 
                165                 170                 175     


Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser Leu Val Ser Lys 
            180                 185                 190         


Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr Phe Gly Gly Gly 
225                 230                 235                 240 


Thr Lys Val Glu Ile Lys 
                245     


<210>  50
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-EGFRvIII scFv

<400>  50
gagattcagc tcgtgcaatc gggagcggaa gtcaagaagc caggagagtc cttgcggatc       60

tcatgcaagg gtagcggctt taacatcgag gattactaca tccactgggt gaggcagatg      120

ccggggaagg gactcgaatg gatgggacgg atcgacccag aaaacgacga aactaagtac      180

ggtccgatct tccaaggcca tgtgactatt agcgccgata cttcaatcaa taccgtgtat      240

ctgcaatggt cctcattgaa agcctcagat accgcgatgt actactgtgc tttcagagga      300

ggggtctact ggggacaggg aactaccgtg actgtctcgt ccggcggagg cgggtcagga      360

ggtggcggca gcggaggagg agggtccggc ggaggtgggt ccgacgtcgt gatgacccag      420

agccctgaca gcctggcagt gagcctgggc gaaagagcta ccattaactg caaatcgtcg      480

cagagcctgc tggactcgga cggaaaaacg tacctcaatt ggctgcagca aaagcctggc      540

cagccaccga agcgccttat ctcactggtg tcgaagctgg attcgggagt gcccgatcgc      600

ttctccggct cgggatcggg tactgacttc accctcacta tctcctcgct tcaagcagag      660

gacgtggccg tctactactg ctggcaggga acccactttc cgggaacctt cggcggaggg      720

acgaaagtgg agatcaag                                                    738


<210>  51
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-FAP scFv

<400>  51

Gln Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Arg Ser Ser Pro Lys Pro Trp Ile Phe 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Arg 
    50                  55                  60                  


Gly Ser Gly Thr Ser Phe Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Pro Ile 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln 
        115                 120                 125             


Pro Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys 
    130                 135                 140                 


Lys Ala Ser Gly Tyr Thr Ile Thr Ser Tyr Ser Leu His Trp Val Lys 
145                 150                 155                 160 


Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Glu Ile Asn Pro Ala 
                165                 170                 175     


Asn Gly Asp His Asn Phe Ser Glu Lys Phe Glu Ile Lys Ala Thr Leu 
            180                 185                 190         


Thr Val Asp Ser Ser Ser Asn Thr Ala Phe Met Gln Leu Ser Arg Leu 
        195                 200                 205             


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Thr Arg Leu Asp Asp Ser 
    210                 215                 220                 


Arg Phe His Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr 
225                 230                 235                 240 


Val Ser Ser 
            


<210>  52
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-FAP scFv

<400>  52
caaattgttc tcacccagtc tccagcgctc atgtctgctt ctccagggga gaaggtcacc       60

atgacctgca ctgccagctc aagtgttagt tacatgtact ggtaccagca gaagccacga      120

tcctccccca aaccctggat ttttctcacc tccaacctgg cttctggagt ccctgctcgc      180

ttcagtggcc gtgggtctgg gacctctttc tctctcacaa tcagcagcat ggaggctgaa      240

gatgctgcca cttattactg ccagcagtgg agtggttacc cacccatcac attcggctcg      300

gggacaaagt tggaaataaa aggtggaggt ggcagcggag gaggtgggtc cggcggtgga      360

ggaagccagg tccaactgca gcagcctggg gctgaactgg taaagcctgg ggcttcagtg      420

aagttgtcct gcaaggcgtc tggctacacc atcaccagct actctctgca ctgggtgaag      480

cagaggcctg gacaaggcct tgagtggatt ggagagatta atcctgccaa tggtgatcat      540

aacttcagtg agaagttcga gatcaaggcc acactgactg tagacagctc ctccaacaca      600

gcattcatgc aactcagcag gctgacatct gaggactctg cggtctatta ctgtacaaga      660

ttggacgata gtaggttcca ctggtacttc gatgtctggg gcgcagggac cacggtcacc      720

gtctcctca                                                              729


<210>  53
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n is an integer of 1

<400>  53

Gly Ser Gly Gly Ser 
1               5   


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeat n times, where n is an integer of 1

<400>  54

Gly Gly Gly Ser 
1               


<210>  55
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n is an integer of 1

<400>  55

Gly Gly Gly Gly Ser 
1               5   


<210>  56
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  56

Gly Gly Ser Gly 
1               


<210>  57
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  57

Gly Gly Ser Gly Gly 
1               5   


<210>  58
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  58

Gly Ser Gly Ser Gly 
1               5   


<210>  59
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  59

Gly Ser Gly Gly Gly 
1               5   


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  60

Gly Gly Gly Ser Gly 
1               5   


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  61

Gly Ser Ser Ser Gly 
1               5   


<210>  62
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  62

Gly Gly Gly Gly Ser 
1               5   


<210>  63
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  63

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  64
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  64
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatct                       45


<210>  65
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  65

Asp Lys Thr His Thr 
1               5   


<210>  66
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  66

Cys Pro Pro Cys 
1               


<210>  67
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  67

Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
1               5                   10                  15  


<210>  68
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  68

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  69
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  69

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  70
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  70

Lys Cys Cys Val Asp Cys Pro 
1               5           


<210>  71
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  71

Lys Tyr Gly Pro Pro Cys Pro 
1               5           


<210>  72
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  72

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  73
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  73

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  74
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  74

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro 
    


<210>  75
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  75

Ser Pro Asn Met Val Pro His Ala His His Ala Gln 
1               5                   10          


<210>  76
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  76

Glu Pro Lys Ser Cys Asp Lys Thr Tyr Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  77
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NY-ESO-1

<400>  77

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


