                         SEQUENCE LISTING

<110>  ILLUMINA, INC.
       ILLUMINA CAMBRIDGE LIMITED

<120>  GENOMIC LIBRARY PREPARATION AND TARGETED EPIGENETIC ASSAYS USING 
       CAS-gRNA RIBONUCLEOPROTEINS

<130>  IP-2061-PCT

<150>  US 63/158,492
<151>  2021-03-09

<150>  US 63/162,775
<151>  2021-03-18

<150>  US 63/163,381
<151>  2021-03-19

<150>  US 63/228,344
<151>  2021-08-02

<150>  US 63/246,879
<151>  2021-09-22

<150>  US 63/295,432
<151>  2021-12-30

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
aatgatacgg cgaccaccga gauctacac                                         29

<210>  2
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n = G or 8-oxoguanine

<400>  2
caagcagaag acggcatacg anat                                              24

<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
aatgatacgg cgaccaccga                                                   20

<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
caagcagaag acggcatacg a                                                 21

<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  5
tttgtccgtg gaatgaacaa                                                   20

<210>  6
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  6
uuuguccgug gaaugaacaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  7
ggcataccat tttatgacgg                                                   20

<210>  8
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  8
ggcauaccau uuuaugacgg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  9
ttacatgaga ctctgcctga                                                   20

<210>  10
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  10
uuacaugaga cucugccuga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  11
ggtcatacca ccggccccaa                                                   20

<210>  12
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  12
ggucauacca ccggccccaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  13
gcgcttaccc caaccaacag                                                   20

<210>  14
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  14
gcgcuuaccc caaccaacag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  15
caccaccaaa gctaactgac                                                   20

<210>  16
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  16
caccaccaaa gcuaacugac guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  17
tgtcctatat caccacaaaa                                                   20

<210>  18
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  18
uguccuauau caccacaaaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100

<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide Sequence (target portion)

<400>  19
agtagttggt aacctgacaa                                                   20

<210>  20
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full guide RNA sequence

<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate linkage between bases 1 and 2, 2 and 3, and 3 
       and 4

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2prime-O-Methyl modification

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  phosphorothioate linkage between bases 97 and 98, 98 and 99, and 
       99 and 100

<400>  20
aguaguuggu aaccugacaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100