                         SEQUENCE LISTING

<110>    Biocytogen PharmaceuticalsBeijingCo., Ltd.
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERIC GCGR GENES

<130>  1

<150>  2021102549598
<151>  2021-03-09

<150>  2021114105634
<151>  2021-11-25

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  485
<212>  PRT
<213>  Mus musculus

<400>  1

Met Pro Leu Thr Gln Leu His Cys Pro His Leu Leu Leu Leu Leu Leu 
1               5                   10                  15      


Val Leu Ser Cys Leu Pro Glu Ala Pro Ser Ala Gln Val Met Asp Phe 
            20                  25                  30          


Leu Phe Glu Lys Trp Lys Leu Tyr Ser Asp Gln Cys His His Asn Leu 
        35                  40                  45              


Ser Leu Leu Pro Pro Pro Thr Glu Leu Val Cys Asn Arg Thr Phe Asp 
    50                  55                  60                  


Lys Tyr Ser Cys Trp Pro Asp Thr Pro Pro Asn Thr Thr Ala Asn Ile 
65                  70                  75                  80  


Ser Cys Pro Trp Tyr Leu Pro Trp Tyr His Lys Val Gln His Arg Leu 
                85                  90                  95      


Val Phe Lys Arg Cys Gly Pro Asp Gly Gln Trp Val Arg Gly Pro Arg 
            100                 105                 110         


Gly Gln Pro Trp Arg Asn Ala Ser Gln Cys Gln Leu Asp Asp Glu Glu 
        115                 120                 125             


Ile Glu Val Gln Lys Gly Val Ala Lys Met Tyr Ser Ser Gln Gln Val 
    130                 135                 140                 


Met Tyr Thr Val Gly Tyr Ser Leu Ser Leu Gly Ala Leu Leu Leu Ala 
145                 150                 155                 160 


Leu Val Ile Leu Leu Gly Leu Arg Lys Leu His Cys Thr Arg Asn Tyr 
                165                 170                 175     


Ile His Gly Asn Leu Phe Ala Ser Phe Val Leu Lys Ala Gly Ser Val 
            180                 185                 190         


Leu Val Ile Asp Trp Leu Leu Lys Thr Arg Tyr Ser Gln Lys Ile Gly 
        195                 200                 205             


Asp Asp Leu Ser Val Ser Val Trp Leu Ser Asp Gly Ala Met Ala Gly 
    210                 215                 220                 


Cys Arg Val Ala Thr Val Ile Met Gln Tyr Gly Ile Ile Ala Asn Tyr 
225                 230                 235                 240 


Cys Trp Leu Leu Val Glu Gly Val Tyr Leu Tyr Ser Leu Leu Ser Leu 
                245                 250                 255     


Ala Thr Phe Ser Glu Arg Ser Phe Phe Ser Leu Tyr Leu Gly Ile Gly 
            260                 265                 270         


Trp Gly Ala Pro Leu Leu Phe Val Ile Pro Trp Val Val Val Lys Cys 
        275                 280                 285             


Leu Phe Glu Asn Val Gln Cys Trp Thr Ser Asn Asp Asn Met Gly Phe 
    290                 295                 300                 


Trp Trp Ile Leu Arg Ile Pro Val Phe Leu Ala Leu Leu Ile Asn Phe 
305                 310                 315                 320 


Phe Ile Phe Val His Ile Ile His Leu Leu Val Ala Lys Leu Arg Ala 
                325                 330                 335     


His Gln Met His Tyr Ala Asp Tyr Lys Phe Arg Leu Ala Arg Ser Thr 
            340                 345                 350         


Leu Thr Leu Ile Pro Leu Leu Gly Val His Glu Val Val Phe Ala Phe 
        355                 360                 365             


Val Thr Asp Glu His Ala Gln Gly Thr Leu Arg Ser Thr Lys Leu Phe 
    370                 375                 380                 


Phe Asp Leu Phe Leu Ser Ser Phe Gln Gly Leu Leu Val Ala Val Leu 
385                 390                 395                 400 


Tyr Cys Phe Leu Asn Lys Glu Val Gln Ala Glu Leu Met Arg Arg Trp 
                405                 410                 415     


Arg Gln Trp Gln Glu Gly Lys Ala Leu Gln Glu Glu Arg Leu Ala Ser 
            420                 425                 430         


Ser His Gly Ser His Met Ala Pro Ala Gly Pro Cys His Gly Asp Pro 
        435                 440                 445             


Cys Glu Lys Leu Gln Leu Met Ser Ala Gly Ser Ser Ser Gly Thr Gly 
    450                 455                 460                 


Cys Val Pro Ser Met Glu Thr Ser Leu Ala Ser Ser Leu Pro Arg Leu 
465                 470                 475                 480 


Ala Asp Ser Pro Thr 
                485 


<210>  2
<211>  477
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Pro Pro Cys Gln Pro Gln Arg Pro Leu Leu Leu Leu Leu Leu Leu 
1               5                   10                  15      


Leu Ala Cys Gln Pro Gln Val Pro Ser Ala Gln Val Met Asp Phe Leu 
            20                  25                  30          


Phe Glu Lys Trp Lys Leu Tyr Gly Asp Gln Cys His His Asn Leu Ser 
        35                  40                  45              


Leu Leu Pro Pro Pro Thr Glu Leu Val Cys Asn Arg Thr Phe Asp Lys 
    50                  55                  60                  


Tyr Ser Cys Trp Pro Asp Thr Pro Ala Asn Thr Thr Ala Asn Ile Ser 
65                  70                  75                  80  


Cys Pro Trp Tyr Leu Pro Trp His His Lys Val Gln His Arg Phe Val 
                85                  90                  95      


Phe Lys Arg Cys Gly Pro Asp Gly Gln Trp Val Arg Gly Pro Arg Gly 
            100                 105                 110         


Gln Pro Trp Arg Asp Ala Ser Gln Cys Gln Met Asp Gly Glu Glu Ile 
        115                 120                 125             


Glu Val Gln Lys Glu Val Ala Lys Met Tyr Ser Ser Phe Gln Val Met 
    130                 135                 140                 


Tyr Thr Val Gly Tyr Ser Leu Ser Leu Gly Ala Leu Leu Leu Ala Leu 
145                 150                 155                 160 


Ala Ile Leu Gly Gly Leu Ser Lys Leu His Cys Thr Arg Asn Ala Ile 
                165                 170                 175     


His Ala Asn Leu Phe Ala Ser Phe Val Leu Lys Ala Ser Ser Val Leu 
            180                 185                 190         


Val Ile Asp Gly Leu Leu Arg Thr Arg Tyr Ser Gln Lys Ile Gly Asp 
        195                 200                 205             


Asp Leu Ser Val Ser Thr Trp Leu Ser Asp Gly Ala Val Ala Gly Cys 
    210                 215                 220                 


Arg Val Ala Ala Val Phe Met Gln Tyr Gly Ile Val Ala Asn Tyr Cys 
225                 230                 235                 240 


Trp Leu Leu Val Glu Gly Leu Tyr Leu His Asn Leu Leu Gly Leu Ala 
                245                 250                 255     


Thr Leu Pro Glu Arg Ser Phe Phe Ser Leu Tyr Leu Gly Ile Gly Trp 
            260                 265                 270         


Gly Ala Pro Met Leu Phe Val Val Pro Trp Ala Val Val Lys Cys Leu 
        275                 280                 285             


Phe Glu Asn Val Gln Cys Trp Thr Ser Asn Asp Asn Met Gly Phe Trp 
    290                 295                 300                 


Trp Ile Leu Arg Phe Pro Val Phe Leu Ala Ile Leu Ile Asn Phe Phe 
305                 310                 315                 320 


Ile Phe Val Arg Ile Val Gln Leu Leu Val Ala Lys Leu Arg Ala Arg 
                325                 330                 335     


Gln Met His His Thr Asp Tyr Lys Phe Arg Leu Ala Lys Ser Thr Leu 
            340                 345                 350         


Thr Leu Ile Pro Leu Leu Gly Val His Glu Val Val Phe Ala Phe Val 
        355                 360                 365             


Thr Asp Glu His Ala Gln Gly Thr Leu Arg Ser Ala Lys Leu Phe Phe 
    370                 375                 380                 


Asp Leu Phe Leu Ser Ser Phe Gln Gly Leu Leu Val Ala Val Leu Tyr 
385                 390                 395                 400 


Cys Phe Leu Asn Lys Glu Val Gln Ser Glu Leu Arg Arg Arg Trp His 
                405                 410                 415     


Arg Trp Arg Leu Gly Lys Val Leu Trp Glu Glu Arg Asn Thr Ser Asn 
            420                 425                 430         


His Arg Ala Ser Ser Ser Pro Gly His Gly Pro Pro Ser Lys Glu Leu 
        435                 440                 445             


Gln Phe Gly Arg Gly Gly Gly Ser Gln Asp Ser Ser Ala Glu Thr Pro 
    450                 455                 460                 


Leu Ala Gly Gly Leu Pro Arg Leu Ala Glu Ser Pro Phe 
465                 470                 475         


<210>  3
<211>  4982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5arm 

<400>  3
agtttctgat cccgaggtta ctgggcagga ggggagatga ggcaacaaaa ggtggctcaa       60

gggctcaaat gggggggcgg gcagtggaga aagtcctctc cagggctgag tctaggagcg      120

aaaggcaggc ttgtggctca gaacctttga ctccagaaaa ggaggtatct aaaaaaggtc      180

tgtgccgact gagaatatgg ccaaattagt ggattgtcaa atgagtggtg ttgcttgagg      240

gcttattgga caggagtccc tgaggcccac agggcatgta acctggaaag ggtttaagag      300

gcactcttag cggatcacct tgtctgaaat ctgcagagcg gcccatgtat cctgacctct      360

cccacccatg gtgcatgtgt ggcagctgca accttccagc caggagccag atgtggttaa      420

gtaagggact tagtccttct agaaaatcaa gactgcaaac ctgaacgcaa tgatgagacc      480

ccaggacagt ggacattgaa agagaaaggg gcgcgctgag tcacttattg ttcccttgga      540

gaactcagac ccatcaggat tcgcacctgc caggtcagga ttcacccgtg gaggctctct      600

gaagtccagg gtgcgcaagg ttggatcctc agggttacgg tacacctggt gagggttctc      660

ggaagggacc cgtagggtcg gatccaggtc agggcctgct cctgtgtgcc agaggggagg      720

gaaagggaga acgcgggatg cagacctgcc aggcaagctc agcccaggag atatactcgg      780

ggttacaccg agagggtctg agagctcgct gcaggtccgc tgcactaagg cgcgggcgcg      840

gaccctcatc tctccccctc ccccgcgggc cgctcccgcc cccacccctc ccccttcgcc      900

gccgcccagc gtcgccgctg gaaagtttgc gagcggctgg cgcggagctg gcgccgaccc      960

cgatcacagc gcggccgagg cgagcagtcg ccggcgccca gatccgagta cgctcgagga     1020

ccgcgaggag cgcagcccta gccccggcga ctgagcacag tgagcactag ggtcgggccc     1080

cgaggggatg gtctcaggct gtctgtgggg tgctggagca cgagcgtgac ctgggcgcac     1140

agaagagggg agcggggagc tggccctcgg gcctggacgt aagccagctg tccctggggt     1200

tttgggagtc gcggttctcg tcccggtcta acaccttatt caaaacggga aatgtgtcct     1260

actgtcacgg ttagggccac ctgattccca accgtctcct gcaggttagg ggtctcaact     1320

tctcctttct attttctggg agcgcaatcc aggaaggtgg gagagggtgg gtgcagacgt     1380

ggatcctgct gccccattga acgcccaaca cactccagct tccagcttct cacactccaa     1440

aggctcgctc atccccttca gatggattcg cttccccttc ctatgtgtcc ctgatctcca     1500

gaacttccta gcagtgtctc agtcttgcgg gttagatgtg taggcaccgt gcactgaggg     1560

ttttttttcc cctgcaagct caggtcattg ctccccagag accaccttcc cacttcagac     1620

ccgcgaagtt ttctgtcgca aaccgactga tcccttacct cttagctctc gcatccttcg     1680

aggttagacc aggcatattt tgctgttctt ctgtcttgga gacatagaag ggggaatctg     1740

agctcacccg agcccgtggg tatctccgcc ccttttcagg ggaggactgg agaactaggc     1800

tttgtcacag cctgtggccc ctgatcctgt ccctgccata ggactatgac ctggtctagt     1860

ttctctgttt tgacaaaaac acccttccag ttgatatctc tggggattcc taagatcaaa     1920

aacgttgcta ctttggcttc taaggaacca agaggatccc ccccccacct tccctcgatg     1980

cctccctttg gccactgggc catccatcta acaccctccg ccacctgcag agaaaatcgg     2040

taaccacact gcaaacaccc acgtgtcctt tgtcctgttg gagcttgctg gaagcggtgg     2100

tggcatggga caatgccagt ccgagtggct ggtgtggtgg tggcatggga caatgccagt     2160

ccgagtggct ggtgtgccag atatgtgctc tgaagaaggg ggattgattg atctaaaggc     2220

aagacaggca agcatcttag ggattcagac ttgcatcttc cgtatttcac ctacagttgg     2280

gtctcactga gagtcagatt tcagcagcct agttcaagac gggaagaggg tagggtccct     2340

ggtctactgt cccttatgtc catctgttcc ctgacaggga gggtaataag gtggaaaaag     2400

gaccagaact gcagctcagg tcacaggtgc gagagcgcgt cgtgcacacc tggctgcctg     2460

tcaccctgag tatttatcag agctgatagg ctctgtctga gtgtcagtat ttgcccaccg     2520

tggggacagg aggcacactg cgaaggtaca gagattgcag tcctatccta ctgacgacat     2580

agcctctctc cttcagtgga agggaaaggg tgccgtgttg ctcttcatac atctctgctc     2640

tcttaaagtc cagacgtttt ctgcagagac cccagagtct caatttcatg gggctgaagt     2700

cagcctcagc ttcctgatgc cagaaatccc ccatcgttat tccatccaca ggaatagaac     2760

actcacccac cttttctgcc acttttgttt gagagacaag gttagaggtt actgaaccct     2820

tagggatcca gccctgagtg gttttgagct cacccactgt ggtctcagtg ctaaactgtg     2880

tgtggaagga aatcctcaag ggttcttggt ctcgcctttg ttactctttg ttatttattt     2940

atttttggat ttttcttttt tcttttgttt gtttgttttg ttttgttttg tgacagggtt     3000

tctctgtgta gccctgggta tccctgacct tactatgtag accaggctag cctcaaactc     3060

agagatcctc ctgtctctgt ctcccgagtc tgggattaaa ggtgtgtgcc accacctatc     3120

ggtcattgaa catttacatt ttcaggttgg gcaactgggc tctaaggacc atcctccccg     3180

actgtcttga tcttcatcct tgcaggatct tggaccacat ccttccccac catgtccctt     3240

ccctgtatct ccactcccca tctacccacg ctggtcatct tttatcacct tcacactgcc     3300

ctgaagcctt cccgcctcta accttcctct ttcaggcctc tatgagaccc cagtagctct     3360

ggtggaggcc aggcatagtg tgtaaagagc cagcttctgg cttttggctt ctccataccc     3420

agcccactaa taatctggga catttaatgc aaattaggaa agtcatgcca cttggtgacc     3480

ccagaggtag ggatacttga accacctaca cctcctcaac ctgtctgctg ccttgtcccc     3540

agcaggaacc ctctactctg agggtgtttt tccctgaggt tcagagacct gaaatcctgg     3600

atcttgcctc tggccagacc ctgttgacca agggcttggc tcaggagtcc ctcagtaggt     3660

tgtgaaccag atgtgactga agggagcata aatgtaggag gtcaggaggg gagccctacc     3720

aagctgctat tcctagaact cagctggtct tattacagca ggctgaaagc cagggctcca     3780

cttttcccca attgggttct tctctctcat ccccaccctc tctcccctca ccccaggggg     3840

tctttgtatg tagtcctggc tggcactgaa ctttgggtag tacacttgct tctacctcct     3900

gaatggtagg actacaggta tgtgccacca tacctggctc tatcttgtat attctgtttg     3960

tgggtacaca ggacataggc ccaactcagc cctaggagct catagtttga cttcttagag     4020

cccccaagaa gctctttgct tttctggcat gagaatccat cagagctgtc ttagttaggg     4080

ttttactgct gtgaacagac accatggcca aggcagctct tataaggatg acatttaatt     4140

ggggctggct tacaggttca gaggttcagt ccattatcat caaggcaaga acatggcagc     4200

atccaggcag gcatggtgca ggaggagctg agagttccat ttcttgttct gaatgcagct     4260

agcggaagaa tggcttccaa gctgctagga caagagtatt aaagcccaca tgcacaatga     4320

cacgcctatt ccaacaaggc catacctcct aatggtgaca cttcctcggc caagaacata     4380

taaaccatca taagagccaa aaaagggtgc tggtgcagag aggggctggt gactaggatc     4440

tgtcacccgt gatgatccca tgtcttaacg aaaacatcag gcagaggtgt ccctcatctg     4500

ggccctggct cggaactgac ctggaatgag ggccaagtgg cgatgcgcct gggtaccaca     4560

cccttactct ctctatccca gggcaggttg cctagctagt tagctttcct acggggctaa     4620

gaataggtgg agatgtctag gctaggacat catagctgag ttgttggtgc cactctgaga     4680

gatttgtgtg accagatagg gaggtgattg ctttcagctg gccaacccca tgtagcggga     4740

aaacagtagc ccatagtcac ctgtctctga ataggcactg tgagccactc acgtgtctcc     4800

tcccgaagct cggctgtcat gaggccttgg gcttggaggc ttggtttggg ggaacaggat     4860

gctgtgtaga ttttctccag actccactat tctggtcctc tgcagcctga ggagaggtgc     4920

acacactctg aggacctagg tgtgcaacct ctgccagatg tggggcgtgg ctacccagag     4980

gc                                                                    4982


<210>  4
<211>  4787
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3 arm 

<400>  4
gggggtgggg agactcaacc agctacctct gttccagttg ctggtaggaa acctggggga       60

ggggtgtaca cagccagcat caggccacgt gggctgtagc tgctcaggca gagccaggct      120

aattgaagca aatgaaggga caggaggcca ctcgggaaag gtaaacagaa tgactcacct      180

gccaggccaa ccactggggc ctgaaggggc cttagcctgc caccctccca atgcctgggc      240

gtagcaggga gggacttgag gagcacggtg ggcgtaaccc cgatgcccca tgggtgtctg      300

gattactagg actgccagga aacaaagggg ctggaagcct gcaaagttgg ctgcatctgc      360

tggcgaccac ggttcttgac tttgcagcct taggcagatc atcccagacc tccacgtgcc      420

aggccccaga tcctgattct actctgagcc ttggacacct aggtgcctgc tgtgggcaca      480

aagccactcc aagccagcac cagagtacat acattctcct tcacgccagc tgagctcagg      540

gttggtgact gccttggggg ttgcagctca cttggggcct cctgacagcc ctccccatcc      600

tgagtctctc tgcccttgtc ctccttcacc ctgtccccat ctctgtcccc aagggaactg      660

gtgcaggctg gttcaggcag ggaaagaggt gggagccagg taggttcacc tagccaatga      720

atatgaacta tgacagacag ctccacagtg gtggggggag tctccagctc agcccaggca      780

tgcagcatcc tttttatttc tggagaccct ttccctcctc catggtactg tcccctcctt      840

cagtcctgcc tatcagtccc cttctcccca gaggaatagc gggtgctacc agcacagtgt      900

gtgagtgtgc acaggtacct tggagggagg gacacaggag tgtgactatg aaggaggtgt      960

gaccctgctg tgtatgctga caggtggggc aggtatataa atgctcaacg ttccagaggg     1020

atgtgagtga tggagctgtg ctaggaggaa gatcacttgc acgtgtttcc ctagagtctg     1080

gtgtgtagat attcctacta ttaaatgacg gcggagaccg aggacacaaa aacaaaacaa     1140

caaaagacac tgaccactga gggtcagact tgggagagtt gagctgtgga ctagaggagg     1200

ggaccttggg ccacaaggct ccagagtctc ctggagaggg tgaaaatgca caggtgggtg     1260

gggctcaggc tctaggatta gtatacagga accagatgtg gtcattccag tacttgccag     1320

gtgaaggcag gaggagaaac tcacgcctgt aactacagaa tacagcaagt caaagactag     1380

cctgggctaa agggacctgc tgtagtcacg gtgcacacct ttaatcccag aacttaggag     1440

gcagaggcag ggtctctgtg actttgagga cagccagggc tatgcagaga gaccccatct     1500

caagcaaaca aacaccagca acaaacccta aagggcagag gacatagagc agtgatagga     1560

tgcctgcctg cctaccacgt gtgaagctgt gtttaatccc ctgggccaca cgaagggcag     1620

gatgcatgct gggtacatgc aaggatgcaa gagcacttcc agagctggga aaacaggagg     1680

atccctgggg ctgcctggcc agtctatcct aactgatgag tgctgcaagc caatcaaaga     1740

cctgtctcaa agggagtggg tgcttctcaa actgctatac acacacacac acacacacac     1800

acacacacac acacacacac acacacacac agagagagag agagaggaga gagagagaga     1860

gagagagaga gagagagaga gagagagaga ggagagagag agagaaaagg aaaagggaag     1920

gagaaattag taattccaag gggcaggaga atatggtcac ttctgactgt gaactcaaac     1980

accaccatgt aactgtccta ttctgatgta accaaggtta caggaagaga aaagtggggg     2040

ctggatcgta gctcagatga tggcgcacct gcccagcagg atctttagca ctacaaactg     2100

gatatggtga cacctaaatt tacaaagttg gaggcaaggg ggtgagaaat tcaaggtcac     2160

cctcagccac acaacaaggg tgaggccacc ttgagatccc atctcaagga aaccaaaaca     2220

aagccactac tgggctagag acagctctgt ggttaagagt gcctggtacc cttggaaggt     2280

ctgggattta gcttctgcca tccgcactgg tcagctcaca actgtctgta actccagtcc     2340

tctgttggct ctacagacac tctcacacat acacacacat aaagagttag ctgggtgttg     2400

tgtcacaccc cttgagtccc agcaccaggt aagcaaaggc aggtggattt ctgcagcttc     2460

aagggctggt ctacacagcc atacagtgag tttcaggcta gccagagctg cttagtgaga     2520

ttttgttttg ttctgataaa cacacacaca cacacacgtt aaaattaata tcatctaaga     2580

ggaagtcact ggtcaggctt acatttcagt ggcagttagg tacatgtgct gtctggcact     2640

gaggtcactc tatggggcta atcttgtcaa gtaaatccat gactgacttg gcagcaggca     2700

ccttgcctgg gaaacaccac aggaagcaag cactcatcac cttaagaggt tggggcatga     2760

caatgttgct gacggtcatg tgtgtggcac cagctcagcc aatgtaggct cactactccc     2820

ggaggggtca ggaagccccc tgtgttctag gacagctggg atgaaatcag caccactagg     2880

aggcagcacg tcctcaggtt aggaccttac ttgctccctc agtggatgga tgcactggta     2940

ttctgggcag tgtgaccact caccacgtcc tggtagcccc taagctgtcc acgccccttc     3000

cagtcctgac aatggggagt tgactgggta acccacagct taggacaccg agtgtcttca     3060

aacagcctaa caggccccag ggaactaaca atgctgagac atggggggcg ggggggtggc     3120

ttggcaccta gaacagctat ttctcactgc cctgtcttgc ctgcgcctcc ctggggagac     3180

ttgccttgcc cggcccacac cctggagatg cctctggggg ccatttgagc cccggaatac     3240

tcttctggga aagcatgttc ctgaaataaa ttccattttg gcatcttctc aggaacagga     3300

gcattccagg gctgtttcca tgattgaagg tcaaatgcga ccaggtctct cttgggtcca     3360

gcagccctgg cctgggcagc caaggagcca acagtgctcc tcagagtcca tagcatggct     3420

gaagagtgct aatgccttcc agagctccac gtgggcaatg taactttact acccaccaga     3480

ctactctggc ggccaggggc ttacagacct gcaaggcagc tctgcgggct tcagggtagg     3540

acctgacaga ctgccatgga gccacttcca gtgccaccgc ctttattcac agaccctcag     3600

tctccaaagc tacagacact tggattcaag agggcacaaa aaaagggggc cttggtaatc     3660

ctcccagcac accccagaaa gccaggacag gcctggcagc accactcctt cagggtcagc     3720

cttaatgccc tgtccaaggg cactgctgtg accacaacag tgtcacaggt accaggacac     3780

aaggcctggt tgccctcaga gagccagcac tgtcctaggc tcagagctga ttcgactctg     3840

aaagtggggc aaggaaaggt caggatggac agtgggggtg gaagcgggag ctccccacat     3900

gtccagggca gaagaagagc aagtctccat ggagagagcc acactggatg caggtgggac     3960

tgcacatgtc ctactttctg gtcatttcaa ggttccaaca gccgaagaaa cagaggtccc     4020

ctttttcctt cctgacctta agtagccagg aaggagaggg gcaaggcatg gcaggtaaca     4080

gcagagaggg ctagataggt cgggccaggt gctacccaag atgcagctgg ccaggggaaa     4140

aggacagggg cggttacagc caggcccctg ttcccccagt gacaggggca acttgagacc     4200

ctggagagga aaggaagggc cctggtcatc aatcctgctg gctctgggat cacggcctgc     4260

tagagcacac tcttcccccc aggagcagcg ccacaggaac acaggaaacc accatcaagt     4320

gcacctgagc ttctggaggc caggggaggg cgccaggagc tctaggactt cttggcatcc     4380

tgcgtgttcc ggcgcttcag gtcactcagg tcgatgggct tgaaggctgt tggtggacag     4440

tctggttggt gctgggaaga ggaccccccc ccccatccgc ttcccactac tagaggctcc     4500

caaacccagg tgccttggaa aggacacagt gcaggcattc agcagaagcc tggtcagctc     4560

ctccaggtcg gccctactca ctcttcaggc tggggttggg gactttctcc acatactcct     4620

ccactaggca ccgctcacca ctggacagct ggctgcgcag gtctcgctca acaccctgcc     4680

aggctgcagc agacaacagg ttcagcgctc agcagtactc aagccaacag agcattctgg     4740

tctcgtgcta ggtcatgtag cccctgctcc tacctgtctc cctctat                   4787


<210>  5
<211>  4671
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a human genomic DNA sequence from GCGR genes  

<400>  5
atgcccccct gccagccaca gcgacccctg ctgctgttgc tgctgctgct ggcctgccag       60

gtgaggactc acagcaccct cagcacccag gggccctcct gtgaggactg cacactgatg      120

gctctctgtc tgcctgcctg cctgcctgcc tgtctgcctg cctgtctgtc tgtctgcccg      180

tctgcctgcc catctgcctg tctgtctgcc tgtccgtctg tctgtccatc tgtccatctg      240

cctatccatc tgcctgcctg tctgcctgtc cgtctgcctg tctgtctgcc tgtccatctg      300

tccatctgcc tatccatctg cctgcctgtc tgtcggcctg cctgcctgcc tgtctgtctg      360

ctgcctgtct gtccgtctgc ctgtctgcct gtccgtctgc ctgcctgtcc gtctgcctgt      420

ccgtctgcct gcctgcctgt ctgtctgcct gcctgtctgc ctgcctgtcc gtctgcctgt      480

ccgtctgcct gcctgtctgc ctgcctgtct gcctgtctgc ccgtctgcct gtctgtctgc      540

ctgtccgtct gcctgtctgt ccgtctgtcc atctgcctat ccatctgcct gcctatctgt      600

ctgtccgtct gcctgcctgt ctgtctgcct gtctgcctgt ctgtctgcct gtctgtccat      660

ctgcctatcc atctacctgc ctgcctgtct gcctgtctgt ctgcctgtct gtctgcctgc      720

ctgtctgtct gtctgtctgg ttgcttgtgc atgtgtcccc cagccacagg tcccctccgc      780

tcaggtgatg gacttcctgt ttgagaagtg gaagctctac ggtgaccagt gtcaccacaa      840

cctgagcctg ctgccccctc ccacgggtga gccccccacc cagagccttt cagcctgtgc      900

ctggcctcag cacttcctga gttctcttca tgggaaggtt cctgggtgct tatgcagcct      960

ttgaggaccc cgccaagggg ccctgtcatt cctcaggccc ccaccaccgt gggcaggtga     1020

ggtaacgagg taactgagcc acagagctgg ggacttgcct caggccgcag agccaggaaa     1080

taacagaacg gtggcattgc cccagaaccg gctgctgctg ctgcccccag gcccagatgg     1140

gtaataccac ctacagcccc gtggagtttt cagtgggcag acagtgccag ggcgtggaag     1200

ctgggaccca ggggcctggg agggctcggg tggagagtgt atatcatggc ctggacactt     1260

ggggtgcagg gagaggatag ggctggagga ctcacccggg aggcagtgcc tgggttcgga     1320

tgagggaggc agccaccact gggcagaggg gggcaggtgt ggcagcctcc attgggcaga     1380

gggagcagat gtggcagcca caggtttggc gatgcacctg ggaaggatga aaatggcatt     1440

ggggttcagc ccccagagag ggaggtgctg agagaaggtc acggagaatg ggggacccca     1500

gtgtgggttt ggggcacatt tgagatgggg ggtctccaag ggaaggtgtc ctgcagagct     1560

gcaattcagg gctgggctgg gcgtgctagc ggaggctggt ccaggggagg tggatggtca     1620

ggtgaggaag gtggaggtca gatgggggag gtggaggtca agtgggggag ggagcagccc     1680

aggccatgtc ctgggcgagg tgacggccga gctcaggctt ccagagagag gagagaggcc     1740

tgctgaggga gccccttctc ccaccctgcc ctgccctgct ctgccctgcc ctaccctacc     1800

ctgcagagct ggtgtgcaac agaaccttcg acaagtattc ctgctggccg gacacccccg     1860

ccaataccac ggccaacatc tcctgcccct ggtacctgcc ttggcaccac aaaggtaccc     1920

atagagggga ggaactgtgg ggggggcggg cccagggtgg ggctgacccc agcctccccc     1980

cacaccccca gtgcaacacc gcttcgtgtt caagagatgc gggcccgacg gtcagtgggt     2040

gcgtggaccc cgggggcagc cttggcgtga tgcctcccag tgccagatgg atggcgagga     2100

gattgaggtc caggtcagtg ggcggcaggc aggcgcggtg gggctggatg ggaacgggca     2160

tgggggcccc tgcctggccc tcacaggcca ctgtaactcg cagaaggagg tggccaagat     2220

gtacagcagc ttccaggtga tgtacacagt gggctacagc ctgtccctgg gggccctgct     2280

cctcgccttg gccatcctgg ggggcctcag gtaggattcc gccagcgccc ggggcggccg     2340

cagaggacag ggaggaggac gggcgctgac tggctgtgcc cacagcaagc tgcactgcac     2400

ccgcaatgcc atccacgcga atctgtttgc gtccttcgtg ctgaaagcca gctccgtgct     2460

ggtcattgat gggctgctca ggacccgcta cagccagaaa attggcgacg acctcagtgt     2520

cagcacctgg ctcagtgatg gagtgagccc ccctcggcgg ccccaggcag gtgggtgggt     2580

gggcagccag gcaggtggcc acgtagccgc gctcacactg cacctgtacc aggcggtggc     2640

tggctgccgt gtggccgcgg tgttcatgca atatggcatc gtggccaact actgctggct     2700

gctggtggag ggcctgtacc tgcacaacct gctgggcctg gccaccctcc ccgagaggag     2760

cttcttcagc ctctacctgg gcatcggctg gggtgagtgg gctggcatga gagggggtta     2820

aggcaggctg accaagcctt tgggaccaca gctgctgccc cccacaggtg cccccatgct     2880

gttcgtcgtc ccctgggcag tggtcaagtg tctgttcgag aacgtccagt gagtatgagc     2940

ggctggacag cctggggagg gaccgggggg ctggggtgcg gcgctctggc ctgaggcagg     3000

gaggggccgg ggatgagcct ggtgcctggg gagggggtca tttgtgacct tctcccttcc     3060

ttttctgaga cccgaattag atcctggcaa aatcgggacg ggggtgctga ggggcggagg     3120

ggctgggggc tgtgccccag tatgtgagtg gcctggcctc gcaggtgctg gaccagcaat     3180

gacaacatgg gcttctggtg gatcctgcgg ttccccgtct tcctggccat cctggtgagg     3240

aaatgaagag ccaggagcgc accccaggcc cctcctccct tggcgtcctg aggctgcccc     3300

aggagacagc agcatcctgt ctgagagcgc tgggagggag ccggcaccca gacaggacac     3360

caggacactg gccagcaccc tggacactga gccaggctgt tcctccctgg ctgtgtgccc     3420

accagcccca gggctatgtg gcccagggcc tatcttgctg ccaggcccac ctgcaggagg     3480

gtcaggtggg gccttccaag ggcacagagc tgttccctgg ggctcgggat gcccctgact     3540

cgcacccttc tcacacagat caacttcttc atcttcgtcc gcatcgttca gctgctcgtg     3600

gccaagctgc gggcacggca gatgcaccac acagactaca agttccggtg ggtgccgcgg     3660

cagctggcgt ctcgagacct ggagaccctc agggccagag ggcagctggg ggtggggact     3720

ccaagctcca cgtggatggt gcgggccgag ggtgggggcg gtgggtgact caggcgctgc     3780

ctctgcaggc tggccaagtc cacgctgacc ctcatccctc tgctgggcgt ccacgaagtg     3840

gtcttcgcct tcgtgacgga cgagcacgcc cagggcaccc tgcgctccgc caagctcttc     3900

ttcgacctct tcctcagctc cttccaggtg cccgcccgcc cgccggctcc cccgcccggg     3960

gcgcagtgtg ccacccctga ccaccctgtc tctccagggc ctgctggtgg ctgtcctcta     4020

ctgcttcctc aacaaggagg taggtgggag tgggggcatc tgagaccatc agcactggcc     4080

gtcggggtca ggggcagaga gaggcacagg gatgccagcc ccacccctgc ccgggggttg     4140

gaacacgtgg ggcccaagcc tttccctccc cctgctctta ttgggtgcag ttgccatggc     4200

gctgggtgtc aggcccccag gacaggttgg cctcagcccc atcgctacgg tgtccaccgt     4260

gggggtcccc aggtgtctgc agactgcttt ccgtggcgat gctgggtggc atagctgtgc     4320

ccagcaggga gcttgtgtcg ctctgcaccc ctcagagcgg agactgggca tctccgatga     4380

ggcccacagc aggtcccggt ggggtggaga ggacaggcag gccctaggac tggcctgccc     4440

cgtccccctc cccaggtgca gtcggagctg cggcggcgtt ggcaccgctg gcgcctgggc     4500

aaagtgctat gggaggagcg gaacaccagc aaccacaggg cctcatcttc gcccggccac     4560

ggccctccca gcaaggagct gcagtttggg aggggtggtg gcagccagga ttcatctgcg     4620

gagaccccct tggctggtgg cctccctaga ttggctgaga gccccttctg a              4671


<210>  6
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 5 end of the Neo cassette and the mouse sequence  

<400>  6
tgtggccaac ctctagggtg gtccctttgg ctgttgctgt gatgcctgcc tagtggagtg       60

tggagatagg gtagatctaa gcttgatatc gaattccgaa gttcctattc tctagaaagt      120

atagg                                                                  125


<210>  7
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 3 end of the Neo cassette and the mouse sequence 

<400>  7
tctagaaagt ataggaactt catcagtcag gtacataatt aggtggatcc gggggtgggg       60

agactcaacc agctacctct gttccagttg ctggtaggaa acctggggga gg              112


<210>  8
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The mRNA sequence of the engineered mouse GCGR after humanization  

<400>  8
gccgaccccg atcacagcgc ggccgaggcg agcagtcgcc ggcgcccaga tccgagtacg       60

ctcgaggacc gcgaggagcg cagccctagc cccggcgact gagcacacct gaggagaggt      120

gcacacactc tgaggaccta ggtgtgcaac ctctgccaga tgtggggcgt ggctacccag      180

aggcatgccc ccctgccagc cacagcgacc cctgctgctg ttgctgctgc tgctggcctg      240

ccagccacag gtcccctccg ctcaggtgat ggacttcctg tttgagaagt ggaagctcta      300

cggtgaccag tgtcaccaca acctgagcct gctgccccct cccacggagc tggtgtgcaa      360

cagaaccttc gacaagtatt cctgctggcc ggacaccccc gccaatacca cggccaacat      420

ctcctgcccc tggtacctgc cttggcacca caaagtgcaa caccgcttcg tgttcaagag      480

atgcgggccc gacggtcagt gggtgcgtgg accccggggg cagccttggc gtgatgcctc      540

ccagtgccag atggatggcg aggagattga ggtccagaag gaggtggcca agatgtacag      600

cagcttccag gtgatgtaca cagtgggcta cagcctgtcc ctgggggccc tgctcctcgc      660

cttggccatc ctggggggcc tcagcaagct gcactgcacc cgcaatgcca tccacgcgaa      720

tctgtttgcg tccttcgtgc tgaaagccag ctccgtgctg gtcattgatg ggctgctcag      780

gacccgctac agccagaaaa ttggcgacga cctcagtgtc agcacctggc tcagtgatgg      840

agcggtggct ggctgccgtg tggccgcggt gttcatgcaa tatggcatcg tggccaacta      900

ctgctggctg ctggtggagg gcctgtacct gcacaacctg ctgggcctgg ccaccctccc      960

cgagaggagc ttcttcagcc tctacctggg catcggctgg ggtgccccca tgctgttcgt     1020

cgtcccctgg gcagtggtca agtgtctgtt cgagaacgtc cagtgctgga ccagcaatga     1080

caacatgggc ttctggtgga tcctgcggtt ccccgtcttc ctggccatcc tgatcaactt     1140

cttcatcttc gtccgcatcg ttcagctgct cgtggccaag ctgcgggcac ggcagatgca     1200

ccacacagac tacaagttcc ggctggccaa gtccacgctg accctcatcc ctctgctggg     1260

cgtccacgaa gtggtcttcg ccttcgtgac ggacgagcac gcccagggca ccctgcgctc     1320

cgccaagctc ttcttcgacc tcttcctcag ctccttccag ggcctgctgg tggctgtcct     1380

ctactgcttc ctcaacaagg aggtgcagtc ggagctgcgg cggcgttggc accgctggcg     1440

cctgggcaaa gtgctatggg aggagcggaa caccagcaac cacagggcct catcttcgcc     1500

cggccacggc cctcccagca aggagctgca gtttgggagg ggtggtggca gccaggattc     1560

atctgcggag acccccttgg ctggtggcct ccctagattg gctgagagcc ccttctgaat     1620

ctccactgga gcctagccag gctgcgttca gaaagggcct cagaggacaa cccagagcca     1680

gatgcccggc caaggctgaa gagacaaagc agcaagacag cagcttgtac tgtgcacact     1740

cccctaacct gtcctagcct ggcacaggcc acagtgacag agtaggggtt ggatatgatg     1800

gagaagccat gttatctatg aactctgagt gttcccatgt gtgttgacat ggtccctgta     1860

cccagatatg tccttcagta aaaagctcga gtgggagctg ctgca                     1905


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  9
caggacatag cgttggctac                                                   20


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  10
gacaccctca ggtagccaag tatgg                                             25


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  11
atctgcaggt atgaggtgga gg                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  12
gacagagagc catcagtgtg ca                                                22


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  13
cagctccctg tcaggatttc tggtg                                             25


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  14
atgtctgtgt cctctcctcc acctc                                             25


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  15
cttcccgcag aggaaggaac aaact                                             25


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  16
cgctgagctt ccggatagat ggttt                                             25


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  17
ggatcggcca ttgaacaaga                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe 

<400>  18
cagaagaact cgtcaagaag                                                   20


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  19
gtcacctgtc tctgaatagg cactg                                             25


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  20
catccaggca gggctacagg ac                                                22


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
gcagacagag agccatcagt gtg                                               23


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  22
aagtccagaa tgggacacac gaagg                                             25


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  23
cttcaattag cctggctctg cctga                                             25


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  24
gacaagcgtt agtaggcaca tatac                                             25


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  25
gctccaattt cccacaacat tagt                                              24


<210>  26
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
tcccctccgc tcaggtgatg gacttc                                            26


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  27
cgaagatgag gccctgtggt tgctg                                             25


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  28
gtgctgtcat gtctgccaga ggca                                              24


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  29
cctcctgaag agctttgcct tcttg                                             25


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  30
tcaccatctt ccaggagcga ga                                                22


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 

<400>  31
gaaggccatg ccagtgagct t                                                 21


<210>  32
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the mouse and human sequences 

<400>  32
gatgtggggc gtggctaccc agaggcatgc ccccctgcca gccacagcga cccct            55


<210>  33
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the human and mouse sequences 

<400>  33
tagattggct gagagcccct tctgaatctc cactggagcc tagccaggct gcgttcagaa       60

agggcc                                                                  66
                        

<210>  34
<211>  485
<212>  PRT
<213>  Rattus norvegicus

<400>  34

Met Leu Leu Thr Gln Leu His Cys Pro Tyr Leu Leu Leu Leu Leu Val 
1               5                   10                  15      


Val Leu Ser Cys Leu Pro Lys Ala Pro Ser Ala Gln Val Met Asp Phe 
            20                  25                  30          


Leu Phe Glu Lys Trp Lys Leu Tyr Ser Asp Gln Cys His His Asn Leu 
        35                  40                  45              


Ser Leu Leu Pro Pro Pro Thr Glu Leu Val Cys Asn Arg Thr Phe Asp 
    50                  55                  60                  


Lys Tyr Ser Cys Trp Pro Asp Thr Pro Pro Asn Thr Thr Ala Asn Ile 
65                  70                  75                  80  


Ser Cys Pro Trp Tyr Leu Pro Trp Tyr His Lys Val Gln His Arg Leu 
                85                  90                  95      


Val Phe Lys Arg Cys Gly Pro Asp Gly Gln Trp Val Arg Gly Pro Arg 
            100                 105                 110         


Gly Gln Ser Trp Arg Asp Ala Ser Gln Cys Gln Met Asp Asp Asp Glu 
        115                 120                 125             


Ile Glu Val Gln Lys Gly Val Ala Lys Met Tyr Ser Ser Tyr Gln Val 
    130                 135                 140                 


Met Tyr Thr Val Gly Tyr Ser Leu Ser Leu Gly Ala Leu Leu Leu Ala 
145                 150                 155                 160 


Leu Val Ile Leu Leu Gly Leu Arg Lys Leu His Cys Thr Arg Asn Tyr 
                165                 170                 175     


Ile His Gly Asn Leu Phe Ala Ser Phe Val Leu Lys Ala Gly Ser Val 
            180                 185                 190         


Leu Val Ile Asp Trp Leu Leu Lys Thr Arg Tyr Ser Gln Lys Ile Gly 
        195                 200                 205             


Asp Asp Leu Ser Val Ser Val Trp Leu Ser Asp Gly Ala Val Ala Gly 
    210                 215                 220                 


Cys Arg Val Ala Thr Val Ile Met Gln Tyr Gly Ile Ile Ala Asn Tyr 
225                 230                 235                 240 


Cys Trp Leu Leu Val Glu Gly Val Tyr Leu Tyr Ser Leu Leu Ser Ile 
                245                 250                 255     


Thr Thr Phe Ser Glu Lys Ser Phe Phe Ser Leu Tyr Leu Cys Ile Gly 
            260                 265                 270         


Trp Gly Ser Pro Leu Leu Phe Val Ile Pro Trp Val Val Val Lys Cys 
        275                 280                 285             


Leu Phe Glu Asn Val Gln Cys Trp Thr Ser Asn Asp Asn Met Gly Phe 
    290                 295                 300                 


Trp Trp Ile Leu Arg Ile Pro Val Leu Leu Ala Ile Leu Ile Asn Phe 
305                 310                 315                 320 


Phe Ile Phe Val Arg Ile Ile His Leu Leu Val Ala Lys Leu Arg Ala 
                325                 330                 335     


His Gln Met His Tyr Ala Asp Tyr Lys Phe Arg Leu Ala Arg Ser Thr 
            340                 345                 350         


Leu Thr Leu Ile Pro Leu Leu Gly Val His Glu Val Val Phe Ala Phe 
        355                 360                 365             


Val Thr Asp Glu His Ala Gln Gly Thr Leu Arg Ser Thr Lys Leu Phe 
    370                 375                 380                 


Phe Asp Leu Phe Phe Ser Ser Phe Gln Gly Leu Leu Val Ala Val Leu 
385                 390                 395                 400 


Tyr Cys Phe Leu Asn Lys Glu Val Gln Ala Glu Leu Leu Arg Arg Trp 
                405                 410                 415     


Arg Arg Trp Gln Glu Gly Lys Ala Leu Gln Glu Glu Arg Met Ala Ser 
            420                 425                 430         


Ser His Gly Ser His Met Ala Pro Ala Gly Thr Cys His Gly Asp Pro 
        435                 440                 445             


Cys Glu Lys Leu Gln Leu Met Ser Ala Gly Ser Ser Ser Gly Thr Gly 
    450                 455                 460                 


Cys Glu Pro Ser Ala Lys Thr Ser Leu Ala Ser Ser Leu Pro Arg Leu 
465                 470                 475                 480 


Ala Asp Ser Pro Thr 
                485 



