                         SEQUENCE LISTING

<110>  University of Louisville Research Foundation, Inc.
 
<120>  ACTINOHIVIN VARIANT POLYPEPTIDES AND RELATED METHODS

<130>  5600.1010001

<150>  63/156,715
<151>  2021-03-04

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  114
<212>  PRT
<213>  Actinomycete

<400>  1

Ala Ser Val Thr Ile Arg Asn Ala Gln Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Gln Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Leu Phe Tyr Ser Asn Gly Tyr 
65                  70                  75                  80  


Ile Gln Asn Val Glu Thr Gly Arg Val Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr Gln Lys Trp Tyr 
            100                 105                 110         


Thr Gly 
        


<210>  2
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 1

<400>  2

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro Ala Asn Gly Gly Ser Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Leu Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Ala Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Leu Leu Asp Ser Asn Tyr Asp Gly 
                85                  90                  95      


Ala Val Tyr Thr Leu Pro Ala Asn Gly Gly Ser Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  3
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 2

<400>  3

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro Cys Asn Gly Gly Ser Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly 
                85                  90                  95      


Ala Val Tyr Thr Leu Pro Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  4
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 3

<400>  4

Ala Ser Val Thr Ile Arg Asn Ala Glu Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Leu Phe Tyr Ser Asn Gly Tyr 
65                  70                  75                  80  


Ile Gln Asn Val Glu Thr Gly Arg Val Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr Gln Lys Trp Tyr 
            100                 105                 110         


Thr Gly 
        


<210>  5
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 4

<400>  5

Ala Ser Val Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Leu Phe Tyr Ser Asn Gly Tyr 
65                  70                  75                  80  


Ile Gln Asn Val Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Tyr 
            100                 105                 110         


Thr Gly 
        


<210>  6
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 5

<400>  6

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Val Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  7
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 6

<400>  7

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asp Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Ser Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Asn Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  8
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 7

<400>  8

Ala Ser Gly Thr Ile Arg Asn Ala Gln Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Gln Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  9
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 8 or Avaren

<400>  9

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  10
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 9

<400>  10

Ala Ser Gly Thr Ile Arg Asn Ala Gln Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  11
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 10

<400>  11

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Gln Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  12
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 11

<400>  12

Ala Ser Gly Thr Ile Arg Asn Ala Gln Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Gln Thr 
        35                  40                  45              


Gly Arg Leu Leu Asp Ser Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro 
    50                  55                  60                  


Ala Asn Gly Gly Asn Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Gln Thr Gly Arg Val Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  13
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 12

<400>  13

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Leu Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Leu Leu Asp Ser Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro 
    50                  55                  60                  


Ala Asn Gly Gly Asn Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Val Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Ala Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  14
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 13

<400>  14

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  15
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant 14

<400>  15

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asp Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Asn Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly 
    


<210>  16
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AvFc

<400>  16

Ala Ser Gly Thr Ile Arg Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser 
1               5                   10                  15      


Asn Tyr Asn Gly Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr 
            20                  25                  30          


Gln Arg Trp Thr Gly Pro Gly Asp Gly Thr Val Arg Asn Ala Glu Thr 
        35                  40                  45              


Gly Arg Cys Leu Asp Ser Asn Tyr Asp Gly Ala Val Tyr Thr Leu Pro 
    50                  55                  60                  


Cys Asn Gly Gly Ser Tyr Gln Lys Trp Thr Gly Pro Gly Asp Gly Thr 
65                  70                  75                  80  


Ile Gln Asn Ala Glu Thr Gly Arg Cys Leu Asp Ser Asn Tyr Asn Gly 
                85                  90                  95      


Asn Val Tyr Thr Leu Pro Cys Asn Gly Gly Asn Tyr Gln Lys Trp Thr 
            100                 105                 110         


Gly Gly Gly Gly Ser Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
        115                 120                 125             


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    130                 135                 140                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
                165                 170                 175     


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        195                 200                 205             


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
    210                 215                 220                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
225                 230                 235                 240 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                245                 250                 255     


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            260                 265                 270         


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
        275                 280                 285             


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    290                 295                 300                 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
305                 310                 315                 320 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
                325                 330                 335     


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 345                 350 


