                         SEQUENCE LISTING

<110>  OREGON HEALTH & SCIENCE UNIVERSITY
 
<120>  Molecular Barcodes and Related Methods and Systems

<130>  Tech 2965

<150>  63/156858
<151>  2021-03-04

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing TUMV cleavage site.


<220>
<221>  SITE
<222>  (17)..(21)
<223>  An azide bearing residue is installed internally near the 
       C-terminus.

<400>  1

Lys Gly Gly Ser Gly Gly Gly Ser Ala Cys Val Tyr His Gln Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Cys 
            20      


<210>  2
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a SuMMV cleavage site.


<220>
<221>  SITE
<222>  (17)..(21)
<223>  An azide bearing residue is installed internally near the 
       C-terminus.

<400>  2

Lys Gly Gly Ser Gly Gly Gly Ser Glu Glu Ile His Leu Gln Ser Gly 
1               5                   10                  15      


Gly Gly Gly Gly Ser Cys 
            20          


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a TVMV cleavage site.


<220>
<221>  SITE
<222>  (17)..(21)
<223>  An azide bearing residue is installed internally near the 
       C-terminus.

<400>  3

Lys Gly Gly Ser Gly Gly Gly Ser Glu Thr Val Arg Phe Gln Gly Gly 
1               5                   10                  15      


Gly Gly Gly Ser Cys 
            20      


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing TEV cleavage site.


<220>
<221>  SITE
<222>  (17)..(21)
<223>  An azide bearing residue is installed internally near the 
       C-terminus.

<400>  4

Lys Gly Gly Ser Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Cys 
            20      


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a HindIII cleavage site.  The 
       nucleotide corresponding to position 19 is modified by Int Amino 
       Modifier C6.

<400>  5
atttatttaa gcttattatt attt                                              24


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a SpeI cleavage site.  The 
       nucleotide corresponding to position 20 is modified by Int Amino 
       Modifier C6.

<400>  6
atttatttaa ctagtattat tattt                                             25


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing an XhoI cleavage site.  The 
       nucleotide corresponding to position 20 is modified by Int Amino 
       Modifier C6.

<400>  7
atttatttac tcgagattat tattt                                             25


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a NotI cleavage site.  The 
       nucleotide corresponding to position 22 is modified by Int Amino 
       Modifier C6.

<400>  8
atttatttag cggccgcatt attattt                                           27


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a TUMV cleavage site.

<400>  9

Lys Gly Gly Ser Gly Gly Gly Ser Ala Cys Val Tyr His Gln Ser Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Cys 
            20          


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a SuMMV cleavage site.

<400>  10

Lys Gly Gly Ser Gly Gly Gly Ser Glu Glu Ile His Leu Gln Ser Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Cys 
            20          


<210>  11
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a TVMV cleavage site.

<400>  11

Lys Gly Gly Ser Gly Gly Gly Ser Glu Thr Val Arg Phe Gln Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Cys 
            20          


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide sequence containing a TEV cleavage site.

<400>  12

Lys Gly Gly Ser Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Ser Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Cys 
            20          


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a HindIII cleavage site.  The 
       nucleotide corresponding to position 1 is modified by a Amino 
       Modifier C6.

<400>  13
catttattta agcttattat tattt                                             25


<210>  14
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a SpeI cleavage site.  The 
       nucleotide corresponding to position 1 is modified by a Amino 
       Modifier C6.

<400>  14
actagtatta ttattt                                                       16


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a XhoI cleavage site.  The 
       nucleotide corresponding to position 1 is modified by a Amino 
       Modifier C6.

<400>  15
atttatttac tcgagattat tattt                                             25


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence containing a NotI cleavage site.  The 
       nucleotide corresponding to position 1 is modified by a Amino 
       Modifier C6.

<400>  16
atttatttag cggccgcatt attattt                                           27


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Biotinylated with 5BiotinTeg at position 1.

<400>  17
attattttat tagctagtac gctacgacgt c                                      31


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 19.  The nucleotides corresponding 
       to positions 1 and 25 are modified, respectively, by 5' 
       phosphoylation and a 3' Amino Modifier C6 dT modified nucleotide.

<400>  18
tgcaggacgt cgtagcgtac tagct                                             25


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 18.  Positions 13-18 comprise a 
       HindIII cleavage site.  Positions 1 is modified by 5Phos.

<400>  19
ctgcacgact gcaagcttga tactagca                                          28


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 21.  Positions 16-21 comprise a 
       HindIII cleavage site.  Positions 1 and 9 are modified by, 
       respectively, 5Phos and iAmMC6T.

<400>  20
acacttgcta gtatcaagct tgcagtcg                                          28


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotides corresponding to positions 1 to 5 comprise a 
       sticky end configured to anneal to the sticky end of SEQ ID NO: 
       20.  Positions 13 to 18 comprise a SpeI recognition moiety and 
       cleavage site.  Position 1 is modified by 5Phos.

<400>  21
agtgtcgact gcactagtga tactagca                                          28


<210>  22
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 23.  Positions 16 to 21 comprise a 
       SpeI recognition moiety and cleavage site.  Positions 1 and 9 are
       modified by, respectively, 5Phos and iAmMC6T.

<400>  22
tatgctgcta gtatcactag tgcagtcg                                          28


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 23.  Positions 13 to 18 comprise a 
       Xho1 recognition moiety and cleavage site.  Position 1 is 
       modified by 5Phos.

<400>  23
gcatacgact gcctcgagga tactagca                                          28


<210>  24
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 25.  Positions 16 to 21 comprise an 
       XhoI recognition moiety and cleavage site.  Positions 1 and 9 are
       modified by, respectively, 5Phos and iAmMC6T.

<400>  24
atggatgcta gtatcctcga ggcagtcg                                          28


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Positions 1 to 5 comprise a sticky end configured to anneal to 
       the sticky end of SEQ ID NO: 24.  Positions 11 to 18 comprise a 
       NotI recognition moiety and cleavage site.  Position 1 is 
       modified by 5Phos.

<400>  25
tccatcgact gcggccgcga tactagca                                          28


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 comprises a 5AmMc6 modified nucleotide.  Positions 11 
       to 18 comprise a NotI recognition moiety and cleavage site.

<400>  26
tgctagtatc gcggccgcag tcg                                               23


<210>  27
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postions 14 and 20 are iAmMC6T 
       modified nucleotides.  Positions 34 to 37 comprise a sticky-end 
       configured to anneal to the sticky end of SEQ ID NO: 28.


<220>
<221>  stem_loop
<222>  (13)..(22)

<400>  27
cgagctcgtc ggtttttttt tccgacgagc tcgaaat                                37


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postion 17 is a  iAmMC6T modified 
       nucleotide.  Positions 34 to 37 comprise a sticky-end configured 
       to anneal to the sticky end of SEQ ID NO: 27.


<220>
<221>  stem_loop
<222>  (13)..(21)

<400>  28
cgcgctcgtc ggtttttttt tccgacgagc gcgattt                                37


<210>  29
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postions 14 and 20 are iAmMC6T 
       modified nucleotides.  Positions 2 to 7 and 27 to 32 are SpeI 
       recognition moieties.  Positions 34 to 37 comprise a sticky-end 
       configured to anneal to the sticky end of SEQ ID NO: 30.


<220>
<221>  stem_loop
<222>  (13)..(21)

<400>  29
cactagtgtc ggtttttttt tccgacacta gtgcaga                                37


<210>  30
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postion 17 is a  iAmMC6T modified 
       nucleotide.  Positions 34 to 37 comprise a sticky-end configured 
       to anneal to the sticky end of SEQ ID NO: 29.


<220>
<221>  stem_loop
<222>  (13)..(21)

<400>  30
cgcgctcgtc ggtttttttt tccgacgagc gcgtctg                                37


<210>  31
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postions 16 and 22 are iAmMC6T 
       modified nucleotides.  Positions 38 to 41 comprise a sticky-end 
       configured to anneal to the sticky end of SEQ ID NO: 32.  
       Positions 2 to 9 and 29 to 36 comprise NotI recognition moieties.


<220>
<221>  stem_loop
<222>  (15)..(23)

<400>  31
cgcggccgcg tcggtttttt tttccgacgc ggccgcgtta c                           41


<210>  32
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Postion 17 is a  iAmMC6T modified 
       nucleotide.  Positions 34 to 37 comprise a sticky-end configured 
       to anneal to the sticky end of SEQ ID NO: 31.


<220>
<221>  stem_loop
<222>  (13)..(21)

<400>  32
cgcgctcgtc ggtttttttt tccgacgagc gcggtaa                                37


<210>  33
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5AmMc6 modified.  Positions 17 to 22 and 31 to 36 
       comprise, respectively, a SpeI regonition moiety and a XhoI 
       recognition moiety.  Positions 41 to 44 comprise a sticky-end 
       configured to anneal to the stick-end of SEQ ID NO: 39.

<400>  33
tttttttatt tatttactag tatttatttc tcgagattta ttt                         43


<210>  34
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Position 14 is a iAmMC6T modified 
       nucleotide. Positions 5 to 10 and 18 to 23 and comprise, 
       respectively, a XhoI recognition moiety and a SpeI recognition 
       moiety.

<400>  34
aaatctcgag aaataaatac tagtaaataa at                                     32


<210>  35
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5AmMc6 modified.  Positions 17 to 22 and 31 to 36 
       comprise, respectively, a HindIII regonition moiety and a SpeI 
       recognition moiety.  Positions 41 to 44 comprise a sticky-end 
       configured to anneal to the stick-end of SEQ ID NO: 39.

<400>  35
tttttttatt tatttaagct tatttattta ctagtattta ttt                         43


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Position 14 is a iAmMC6T modified 
       nucleotide.  Positions 5 to 10 and 18 to 23 and comprise, 
       respectively, a HindIII recognition moiety and a SpeI recognition
       moiety.

<400>  36
aaatactagt aaataaataa gcttaaataa at                                     32


<210>  37
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5AmMc6 modified.  Positions 16 to 21 and 30 to 35 
       comprise, respectively, a EcoRI regonition moiety and a HindIII 
       recognition moiety.  Positions 40 to 43 comprise a sticky-end 
       configured to anneal to the stick-end of SEQ ID NO: 39.

<400>  37
tttttttatt tatttgaatt catttattta agcttattta ttt                         43


<210>  38
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Position 14 is a iAmMC6T modified 
       nucleotide.

<400>  38
aaataagctt aaataaatga attcaaataa at                                     32


<210>  39
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 is 5Phos modified.  Positions 14 and 20 are iAmMc6T 
       modified nucleotide.  Positions 13 to 21 comprise a stem-loop 
       structure.


<220>
<221>  stem_loop
<222>  (13)..(21)

<400>  39
cgcgctcgtc ggtttttttt tccgacgagc gcgaaat                                37


<210>  40
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.  Positions 22 to 27 comprise a 
       XhoI recognition moiety.

<400>  40
tttttttatt tattttccga cgagctcagc attt                                   34


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.  Positions 8 to 13 comprise a SpeI
       recognition moiety.

<400>  41
aaatgctgag ctcgtcggaa                                                   20


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.  Positions 21 to 26 comprise a 
       SpeI recognition moiety.

<400>  42
tttttttatt tattttagat actagtgata ctt                                    33


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.

<400>  43
aagtatcact agtatctaaa                                                   20


<210>  44
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.  Positions 21 to 26 comprise a 
       NotI recognition

<400>  44
tttttttatt tattttcata gcggccgcgt atctt                                  35


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Position 1 modified by 5AmMC6.

<400>  45
aagatacgcg gccgctatga aa                                                22


