                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  METHODS FOR GENERATING A CRISPR ARRAY

<130>  15670-0335WO1

<150>  US 63/155,103
<151>  2021-03-01

<160>  96    

<170>  PatentIn version 3.5

<210>  1
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  1
ttttgactta actctagttc gtcatcgcat agatgattta gaaaggtcga tcagggagga       60


<210>  2
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  2
tatcggggaa gaacaggttc gtcatcgcat agatgattta gaaattgcat tctaaaacct       60


<210>  3
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  3
taaatacaga aaacaggttc gtcatcgcat agatgattta gaaagtcgat actatgttat       60


<210>  4
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  4
acgccaactt tgaaaagttc gtcatcgcat agatgattta gaaaaagcga gctcggtact       60


<210>  5
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  5
aaaacaattc atccaggttc gtcatcgcat agatgattta gaaacggccg gtagaaagga       60


<210>  6
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  6
gaacctgttc ttccccgata tcctccctga tcgacctttc                             40


<210>  7
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  7
gaacctgttt tctgtattta aggttttaga atgcaatttc                             40


<210>  8
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  8
gaacttttca aagttggcgt ataacatagt atcgactttc                             40


<210>  9
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  9
gaacctggat gaattgtttt agtaccgagc tcgctttttc                             40


<210>  10
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  10
ttttgactta actctagttc gtcatcgcat agatgattta gaaatcgccg tcgggcatgc       60


<210>  11
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  11
gcgccttgag cctggcgttc gtcatcgcat agatgattta gaaaggctac ctgcccattc       60


<210>  12
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  12
gaccaccaag cgaaacgttc gtcatcgcat agatgattta gaaacaacct taccagaggg       60


<210>  13
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  13
cgccccagct ggcaatgttc gtcatcgcat agatgattta gaaaggccgc ttgggtggag       60


<210>  14
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  14
aggctattcg gctatggttc gtcatcgcat agatgattta gaaacggccg gtagaaagga       60


<210>  15
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  15
gaacgccagg ctcaaggcgc gcatgcccga cggcgatttc                             40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  16
gaacgtttcg cttggtggtc gaatgggcag gtagcctttc                             40


<210>  17
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  17
gaacattgcc agctggggcg ccctctggta aggttgtttc                             40


<210>  18
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  18
gaaccatagc cgaatagcct ctccacccaa gcggcctttc                             40


<210>  19
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  19
tcctttctac cggccgtttc taaatcatct                                        30


<210>  20
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  20
tttggtctca tcctttctac cggccgtttc taaatcatct                             40


<210>  21
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  21
aaaacaattc atccaggttc gtcatcgcat agatgattta gaaatcgccg tcgggcatgc       60


<210>  22
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  22
tagagttaag tcaaaacaaa accc                                              24


<210>  23
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  23
gaaacggccg gtagaaagga                                                   20


<210>  24
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  24
tttggtctca gcgatgacga actagagtta agtcaaaaca aaaccc                      46


<210>  25
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  25
tttggtctca gttcgtcatc gcatagatga tttagaaacg gccggtagaa aggagaag         58


<210>  26
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  26
tgagccgaca ttttattacc ctct                                              24


<210>  27
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  27
ttacctgaaa gccaatcgct g                                                 21


<210>  28
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  28
tttggtctca tcgcatagat gatttagaaa cggaattcaa ggggac                      46


<210>  29
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  29
aggtagcgca ggtgatgttc gtcatcgcat agatgattta gaaaatcgcg cgttacctcc       60


<210>  30
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  30
tgaacatcct ctacaggttc gtcatcgcat agatgattta gaaagagaag tgaacttgtc       60


<210>  31
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  31
gaacatcacc tgcgctacct gtccccttga attccgtttc                             40


<210>  32
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  32
gaacctgtag aggatgttca ggaggtaacg cgcgattttc                             40


<210>  33
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  33
tttggtctca gaacttgaaa ttggtttatc gacaagttca cttctctttc                  50


<210>  34
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  34
tttggtctca tcgcatagat gatttagaaa tctccgcgct tgcttc                      46


<210>  35
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  35
gcataatgca gattgagttc gtcatcgcat agatgattta gaaagtcact atgaccatgt       60


<210>  36
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  36
tgctttgtat tgtgaagttc gtcatcgcat agatgattta gaaacccgga ttttgactgg       60


<210>  37
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  37
cgaaatgtag aagatagttc gtcatcgcat agatgattta gaaacggaat tcaaggggac       60


<210>  38
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  38
gaactcaatc tgcattatgc gaagcaagcg cggagatttc                             40


<210>  39
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  39
gaacttcaca atacaaagca acatggtcat agtgactttc                             40


<210>  40
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  40
gaactatctt ctacatttcg ccagtcaaaa tccgggtttc                             40


<210>  41
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  41
ggagttctga ggtcattact ggatcta                                           27


<210>  42
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  42
caaatgtacg gccagcaacg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  43
agcagctgag agcctgaatg                                                   20


<210>  44
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  44
acatgccagc acttaatctg a                                                 21


<210>  45
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  45
tcagattaag tgctggcatg tgcacccaat ccctaacatt aaaca                       45


<210>  46
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  46
ggttcgggca cctcatcatt                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  47
acagggcagc cattaactga                                                   20


<210>  48
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  48
tctgagactg tagcctacgc a                                                 21


<210>  49
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  49
tgcgtaggct acagtctcag aacgaagtta tgtgccacaa gaaa                        44


<210>  50
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  50
tcagacgcaa gcgtgaagat                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  51
gcctcctaaa attgggggct                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  52
cttggttctg cattgggtgc                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  53
gacttgcgta ggcttggact                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  54
gcatgtcatg gtttggtggg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  55
atgaacgcga tcattgcagc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  56
tacggccaat tgatcaccca                                                   20


<210>  57
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  57
tttggtctca taagaacttt aaataatttc tactgttgta gatcggcgtc aatacggga        59


<210>  58
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  58
taataccgcg ccacatgtct aagaacttta aataatttct actgttgtag atggagctga       60

atgaagcc                                                                68


<210>  59
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  59
ataccaaacg acgagcgtct aagaacttta aataatttct actgttgtag atctcccgta       60

tcgtagtt                                                                68


<210>  60
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  60
atctacacga cggggagtct aagaacttta aataatttct actgttgtag atagccggaa       60

gggccgag                                                                68


<210>  61
<211>  64
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  61
tttggtctca gtctaagaac tttaaataat ttctactgtt gtagatcggc cggtagaaag       60

gaca                                                                    64


<210>  62
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  62
tttggtctca cttagactag agttaagtca aaacaaaacc c                           41


<210>  63
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  63
agacatgtgg cgcggtatta tcccgtattg acgccgatct                             40


<210>  64
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  64
agacgctcgt cgtttggtat ggcttcattc agctccatct                             40


<210>  65
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  65
agactccccg tcgtgtagat aactacgata cgggagatct                             40


<210>  66
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  66
tttggtctca agaccaggac cacttctgcg ctcggccctt ccggctatct                  50


<210>  67
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  67
ggtcgatcag ggaggatatc ggggaagaac ag                                     32


<210>  68
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  68
gtcgatacta tgttatacgc caactttgaa aa                                     32


<210>  69
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  69
ttgcattcta aaaccttaaa tacagaaaac ag                                     32


<210>  70
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  70
aagcgagctc ggtactaaaa caattcatcc ag                                     32


<210>  71
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  71
tcgccgtcgg gcatgcgcgc cttgagcctg gc                                     32


<210>  72
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  72
caaccttacc agagggcgcc ccagctggca at                                     32


<210>  73
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  73
ggctacctgc ccattcgacc accaagcgaa ac                                     32


<210>  74
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  74
ggccgcttgg gtggagaggc tattcggcta tg                                     32


<210>  75
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  75
tctccgcgct tgcttcgcat aatgcagatt ga                                     32


<210>  76
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  76
gtcactatga ccatgttgct ttgtattgtg aa                                     32


<210>  77
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  77
cccggatttt gactggcgaa atgtagaaga ta                                     32


<210>  78
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  78
cggaattcaa ggggacaggt agcgcaggtg at                                     32


<210>  79
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  79
atcgcgcgtt acctcctgaa catcctctac ag                                     32


<210>  80
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  80
gagaagtgaa cttgtcgata aaccaatttc aa                                     32


<210>  81
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR spacers

<400>  81
taggggaaag cctactagcc ggagtgttgc ga                                     32


<210>  82
<211>  263
<212>  PRT
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  82

Met Arg Glu Ala Val Ile Ala Glu Val Ser Thr Gln Leu Ser Glu Val 
1               5                   10                  15      


Val Gly Val Ile Glu Arg His Leu Glu Pro Thr Leu Leu Ala Val His 
            20                  25                  30          


Leu Tyr Gly Ser Ala Val Asp Gly Gly Leu Lys Pro His Ser Asp Ile 
        35                  40                  45              


Asp Leu Leu Val Thr Val Thr Val Arg Leu Asp Glu Thr Thr Arg Arg 
    50                  55                  60                  


Ala Leu Ile Asn Asp Leu Leu Glu Thr Ser Ala Ser Pro Gly Glu Ser 
65                  70                  75                  80  


Glu Ile Leu Arg Ala Val Glu Val Thr Ile Val Val His Asp Asp Ile 
                85                  90                  95      


Ile Pro Trp Arg Tyr Pro Ala Lys Arg Glu Leu Gln Phe Gly Glu Trp 
            100                 105                 110         


Gln Arg Asn Asp Ile Leu Ala Gly Ile Phe Glu Pro Ala Thr Ile Asp 
        115                 120                 125             


Ile Asp Leu Ala Ile Leu Leu Thr Lys Ala Arg Glu His Ser Val Ala 
    130                 135                 140                 


Leu Val Gly Pro Ala Ala Glu Glu Leu Phe Asp Pro Val Pro Glu Gln 
145                 150                 155                 160 


Asp Leu Phe Glu Ala Leu Asn Glu Thr Leu Thr Leu Trp Asn Ser Pro 
                165                 170                 175     


Pro Asp Trp Ala Gly Asp Glu Arg Asn Val Val Leu Thr Leu Ser Arg 
            180                 185                 190         


Ile Trp Tyr Ser Ala Val Thr Gly Lys Ile Ala Pro Lys Asp Val Ala 
        195                 200                 205             


Ala Asp Trp Ala Met Glu Arg Leu Pro Ala Gln Tyr Gln Pro Val Ile 
    210                 215                 220                 


Leu Glu Ala Arg Gln Ala Tyr Leu Gly Gln Glu Glu Asp Arg Leu Ala 
225                 230                 235                 240 


Ser Arg Ala Asp Gln Leu Glu Glu Phe Val His Tyr Val Lys Gly Glu 
                245                 250                 255     


Ile Thr Lys Val Val Gly Lys 
            260             


<210>  83
<211>  3872
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  83
tgagccgaca ttttattacc ctcttatcaa accgtacctt tcacataacg aatgaatgaa       60

taccgtacat ggagtgcggc caacccacag cgaacatcat atttcgcatc catcaccgta      120

cggttttccg ttttaagctc tgcccatgat ctatcatgga aataacggct aatgatcacc      180

tgcatccact caagtgtcgt ttcactgtct gtaccattaa taatatccag tactaaacgt      240

tgtacggcac gagcttcatt atcgttaatc tgacacgaca ctttgtgacg tatagcttgt      300

tgtactcctt gagcatcaca aaggtaataa gcaataagtt tagctcgatc tttcttcttt      360

acacgtacct tggctttctt cattgcaata gcaatcgggc tatcggaata ctgtccacca      420

cgacaagaac gttgccacgc tccacattgg cgtaaccaat ctggcaaatc atacttgctc      480

caatccaccg tctgcataat gtgcactgct gtattcatct catcacctaa tttgtttcaa      540

gttaaatttt ataagcgtta ttgttttatg gttctgcctg ctcctctacc gatctaaaac      600

gacaagtttc gagataatcc agtactcgaa ctgcaccgcg tttaccgtgt cggtttttca      660

ctacaatcag ctctgtgatt cccatcggtt tggttgagtc ttctggatcg gttagggggt      720

taacaaggat gatctggtct gcatcttgct cgatttgtcc agattctttg atatctgatg      780

ctttaggacg tttgcccttc tctgcctcac ggttgagctg taccagtgca atgacaggac      840

attcaaactc tttcgccatg gattttaatt cacggctgat ggaactgact tcctgaaagc      900

gatctttctt gctcgggtct ctgagcagtt gtaaataatc cacgatgatg cagcccaatt      960

ccttgtaacg gcgtttggct cgacgtgcat aggaacggac ctcactcaag tgattcataa     1020

cgaagtattt ttactcatta aaagcttata taattgatat caagggtttt gttttgactt     1080

aactctagtt cgtcatcgca tagatgattt agaaaggtcg atcagggagg atatcgggga     1140

agaacaggtt cgtcatcgca tagatgattt agaaattgca ttctaaaacc ttaaatacag     1200

aaaacaggtt cgtcatcgca tagatgattt agaaagtcga tactatgtta tacgccaact     1260

ttgaaaagtt cgtcatcgca tagatgattt agaaaaagcg agctcggtac taaaacaatt     1320

catccaggtt cgtcatcgca tagatgattt agaaatcgcc gtcgggcatg cgcgccttga     1380

gcctggcgtt cgtcatcgca tagatgattt agaaaggcta cctgcccatt cgaccaccaa     1440

gcgaaacgtt cgtcatcgca tagatgattt agaaacaacc ttaccagagg gcgccccagc     1500

tggcaatgtt cgtcatcgca tagatgattt agaaaggccg cttgggtgga gaggctattc     1560

ggctatggtt cgtcatcgca tagatgattt agaaacggcc ggtagaaagg agaagcttac     1620

tagctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac     1680

cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagggaagcg gtgatcgccg     1740

aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt     1800

tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata     1860

ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca     1920

acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag     1980

tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc     2040

aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg     2100

acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc     2160

cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg     2220

aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc     2280

ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg     2340

ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta     2400

gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag     2460

aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa     2520

ttcgttcaag ccgaggggcc gcaagatccg gccacgatga cccggtcgtc ggttcagggc     2580

agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa     2640

gcagtgtgac cgtgtgcttc tcaaatgcct gaggtttcag caaaaaaccc ctcaagaccc     2700

gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gcctaggtta     2760

attaagctgc gctagtagac gagtccatgt gctggcgttc aaatttcgca gcagcggttt     2820

ctttaccaga ctcgacaagc ttactagagt gttcatattg acctcgctta gtgtggttaa     2880

tacgccgctt cttgttactg caagaggcgg tttttttatg ggtgtacaca tgactgcacc     2940

tgttgattca gttcagatag tgctttgtgc atctcatgga tgactctggc catatccagt     3000

gcttcgcctt gggtaattcg cccatcggcc atcatttctt taaacagtgc tgatatatcg     3060

cccttcttga tgcctatgca taggaaggta tccatcagac tggtatctcg ctggctctcg     3120

ggtatgtctg gcaggtcaat tgccaccttt ccgagtcgtg cacacatttc ctgcaatatc     3180

cgatagtccc ctgtaatctc catcagcttg actgcctcga gcaatgtaat gtgatgggta     3240

tgtgtgtttg ggttgacctt gctattgagc accgcagggc ttttgatgcc taaacgtgat     3300

gcaagtgcag atgcaccacc cagaaagtcg tgaacggtgt gataggcagc atctaatatg     3360

ttcatggcgg gttcctttga acgtgtttat tagatgggtg ctgacataag attggattta     3420

tggttaggac gtaattcaat ccaaatatct tcgtaatcat ctggaaataa atctttgcga     3480

gtgcatagac ctttatcttc agcaattaca gctaagcgga ttttgcgatc tctgggaatt     3540

gctttccatc cacttacgga tgcagcggtg atacctaaaa atctagcaac agcagttaca     3600

ccacctaaaa gctcaataaa ttgatcatca gtcatgttga tctcctaatt ttattgcctc     3660

aattattagg tattccttat attttatcaa taggaatacc ttatttattt tatgttagga     3720

tttcctaata gactaggtaa gatcatgaaa acattagctg aacgacttaa atatgcgatg     3780

gaaattttgc cacctaagaa aatcaagggt gtcgaacttg ctcgtgtagt tggagttaaa     3840

ccaccatctg tcagcgattg gctttcaggt aa                                   3872


<210>  84
<211>  232
<212>  PRT
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  84

Met Ala Ile Lys Asn Thr Lys Ala Arg Asn Phe Gly Phe Leu Leu Tyr 
1               5                   10                  15      


Pro Asp Ser Ile Pro Asn Asp Trp Lys Glu Lys Leu Glu Ser Leu Gly 
            20                  25                  30          


Val Ser Met Ala Val Ser Pro Leu His Asp Met Asp Glu Lys Lys Asp 
        35                  40                  45              


Lys Asp Thr Trp Asn Ser Ser Asp Val Ile Arg Asn Gly Lys His Tyr 
    50                  55                  60                  


Lys Lys Pro His Tyr His Val Ile Tyr Ile Ala Arg Asn Pro Val Thr 
65                  70                  75                  80  


Ile Glu Ser Val Arg Asn Lys Ile Lys Arg Lys Leu Gly Asn Ser Ser 
                85                  90                  95      


Val Ala His Val Glu Ile Leu Asp Tyr Ile Lys Gly Ser Tyr Glu Tyr 
            100                 105                 110         


Leu Thr His Glu Ser Lys Asp Ala Ile Ala Lys Asn Lys His Ile Tyr 
        115                 120                 125             


Asp Lys Lys Asp Ile Leu Asn Ile Asn Asp Phe Asp Ile Asp Arg Tyr 
    130                 135                 140                 


Ile Thr Leu Asp Glu Ser Gln Lys Arg Glu Leu Lys Asn Leu Leu Leu 
145                 150                 155                 160 


Asp Ile Val Asp Asp Tyr Asn Leu Val Asn Thr Lys Asp Leu Met Ala 
                165                 170                 175     


Phe Ile Arg Leu Arg Gly Ala Glu Phe Gly Ile Leu Asn Thr Asn Asp 
            180                 185                 190         


Val Lys Asp Ile Val Ser Thr Asn Ser Ser Ala Phe Arg Leu Trp Phe 
        195                 200                 205             


Glu Gly Asn Tyr Gln Cys Gly Tyr Arg Ala Ser Tyr Ala Lys Val Leu 
    210                 215                 220                 


Asp Ala Glu Thr Gly Glu Ile Lys 
225                 230         


<210>  85
<211>  53
<212>  PRT
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  85

Met Val Ile Ser Glu Ser Lys Lys Arg Val Met Ile Ser Leu Thr Lys 
1               5                   10                  15      


Glu Gln Asp Lys Lys Leu Thr Asp Met Ala Lys Gln Lys Gly Phe Ser 
            20                  25                  30          


Lys Ser Ala Val Ala Ala Leu Ala Ile Glu Glu Tyr Ala Arg Lys Glu 
        35                  40                  45              


Ser Glu Gln Lys Lys 
    50              


<210>  86
<211>  69
<212>  PRT
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  86

Met Gly Gly Lys Glu Ala Asn Phe Ala Ser Val Leu Arg Pro Pro Ile 
1               5                   10                  15      


Lys Cys Arg Val Pro Ile Phe Val Pro Lys Thr Leu Tyr Pro Asn Trp 
            20                  25                  30          


Leu Lys Gly Leu Arg Gly Phe Ser Ile Ala Asn Glu Ser Pro Thr Phe 
        35                  40                  45              


Ser Pro Thr Phe Phe Ile Asn Leu Tyr Leu Ser Ser Phe Ile Val Val 
    50                  55                  60                  


Phe Met Ile Thr Lys 
65                  


<210>  87
<211>  263
<212>  PRT
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  87

Met Arg Glu Ala Val Ile Ala Glu Val Ser Thr Gln Leu Ser Glu Val 
1               5                   10                  15      


Val Gly Val Ile Glu Arg His Leu Glu Pro Thr Leu Leu Ala Val His 
            20                  25                  30          


Leu Tyr Gly Ser Ala Val Asp Gly Gly Leu Lys Pro His Ser Asp Ile 
        35                  40                  45              


Asp Leu Leu Val Thr Val Thr Val Arg Leu Asp Glu Thr Thr Arg Arg 
    50                  55                  60                  


Ala Leu Ile Asn Asp Leu Leu Glu Thr Ser Ala Ser Pro Gly Glu Ser 
65                  70                  75                  80  


Glu Ile Leu Arg Ala Val Glu Val Thr Ile Val Val His Asp Asp Ile 
                85                  90                  95      


Ile Pro Trp Arg Tyr Pro Ala Lys Arg Glu Leu Gln Phe Gly Glu Trp 
            100                 105                 110         


Gln Arg Asn Asp Ile Leu Ala Gly Ile Phe Glu Pro Ala Thr Ile Asp 
        115                 120                 125             


Ile Asp Leu Ala Ile Leu Leu Thr Lys Ala Arg Glu His Ser Val Ala 
    130                 135                 140                 


Leu Val Gly Pro Ala Ala Glu Glu Leu Phe Asp Pro Val Pro Glu Gln 
145                 150                 155                 160 


Asp Leu Phe Glu Ala Leu Asn Glu Thr Leu Thr Leu Trp Asn Ser Pro 
                165                 170                 175     


Pro Asp Trp Ala Gly Asp Glu Arg Asn Val Val Leu Thr Leu Ser Arg 
            180                 185                 190         


Ile Trp Tyr Ser Ala Val Thr Gly Lys Ile Ala Pro Lys Asp Val Ala 
        195                 200                 205             


Ala Asp Trp Ala Met Glu Arg Leu Pro Ala Gln Tyr Gln Pro Val Ile 
    210                 215                 220                 


Leu Glu Ala Arg Gln Ala Tyr Leu Gly Gln Glu Glu Asp Arg Leu Ala 
225                 230                 235                 240 


Ser Arg Ala Asp Gln Leu Glu Glu Phe Val His Tyr Val Lys Gly Glu 
                245                 250                 255     


Ile Thr Lys Val Val Gly Lys 
            260             


<210>  88
<211>  3162
<212>  DNA
<213>  Artificial

<220>
<223>  CRISPR vector

<400>  88
tagaaaggag aagcttacta gtagcggccg ctgcaggcct cagggcccga tcgatgccgc       60

cgcttaatta attaatccag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg      120

cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc      180

cctagaccta gtgtcatttt atttcccccg tttcagcatc aagaaccttt gcataacttg      240

ctctatatcc acactgataa ttgccctcaa accataatct aaaggcgcta gagtttgttg      300

aaacaatatc ttttacatca ttcgtattta aaattccaaa ctccgctccc ctaaggcgaa      360

taaaagccat taaatctttt gtatttacca aattatagtc atccactata tctaagagta      420

aattcttcaa ttctcttttt tggctttcat caagtgttat atagcggtca atatcaaaat      480

cattaatgtt caaaatatct tttttgtcgt atatatgttt attcttagca atagcgtcct      540

ttgattcatg agtcaaatat tcatatgaac ctttgatata atcaagtatc tcaacatgag      600

caactgaact attccccaat tttcgcttaa tcttgttcct aacgctttct attgttacag      660

gatttcgtgc aatatatata acgtgatagt gtggtttttt atagtgcttt ccatttcgta      720

taacatcact actattccat gtatctttat cttttttttc gtccatatcg tgtaaaggac      780

tgacagccat agatacgccc aaactctcta atttttcctt ccaatcatta ggaattgagt      840

caggatataa taaaaatcca aaatttctag ctttagtatt tttaatagcc atgatataat      900

taccttatca aaaacaagta gcgaaaactc gtatccttct aaaaacgcga gctttcgctt      960

attttttttg ttctgattcc tttcttgcat attcttctat agctaacgcc gcaaccgcag     1020

attttgaaaa acctttttgt ttcgccatat ctgttaattt tttatcttgc tcttttgtca     1080

gagaaatcat aactcttttt ttcgattctg aaatcaccat ttaaaaaact ccaatcaaat     1140

aattttataa agttagtgta tcactttgta atcataaaaa caacaataaa gctacttaaa     1200

tatagattta taaaaaacgt tggcgaaaac gttggcgatt cgttggcgat tgaaaaaccc     1260

cttaaaccct tgagccagtt gggatagagc gtttttggca caaaaattgg cactcggcac     1320

ttaatggggg gtcgtagtac ggaagcaaaa ttcgcttcct ttccccccat ttttttccaa     1380

attccaaatt tttttcaaaa attttccagc gctaccgctc ggcaaaattg caagcaattt     1440

ttaaaatcaa acccatgagg gaatttcatt ccctcatact cccttgagcc tcctccaacc     1500

gaaatagaag ggcgctgcgc ttattatttc attcagtcat cggctttcat aatctaacag     1560

acaacatctt cgctgcaaag ccacgctacg ctcaagggct tttacgctac gataacgcct     1620

gttttaacga ttatgccgat aactaaacga aataaacgct aaaacgtctc agaaacgatt     1680

ttgagacgtt ttaataaaaa atcgcctagt gcttggattc tcaccaataa aaaacgcccg     1740

gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctac     1800

aagtgattca taacgaagta tttttactca ttaaaagctt atataattga tatcaagggt     1860

tttgttttga cttaactcta gttcgtcatc gcatagatga tttagaaatc tccgcgcttg     1920

cttcgcataa tgcagattga gttcgtcatc gcatagatga tttagaaagt cactatgacc     1980

atgttgcttt gtattgtgaa gttcgtcatc gcatagatga tttagaaacc cggattttga     2040

ctggcgaaat gtagaagata gttcgtcatc gcatagatga tttagaaacg gccggtagaa     2100

aggagaagct tactagctat ttgtttattt ttctaaatac attcaaatat gtatccgctc     2160

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagggaa     2220

gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat     2280

ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag     2340

ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg     2400

cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc     2460

cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct     2520

aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag     2580

ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt     2640

gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt     2700

gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag     2760

cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg     2820

ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc     2880

atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca     2940

gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa     3000

taatgtctaa caattcgttc aagccgaggg gccgcaagat ccggccacga tgacccggtc     3060

gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta     3120

ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cc                        3162


<210>  89
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  89
tttggtctca gtctaagaac tttaaataat ttctactgtt gtagatcggc gtcaatacgg       60

ga                                                                      62


<210>  90
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  90
taataccgcg ccacatgtct aagaacttta aataatttct actgttgtag atggagctga       60

atgaagcc                                                                68


<210>  91
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  91
ataccaaacg acgagcgtct aagaacttta aataatttct actgttgtag atagccggaa       60

gggccgag                                                                68


<210>  92
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  92
atgtggcgcg gtattatccc gtattgacgc cgatct                                 36


<210>  93
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  93
gctcgtcgtt tggtatggct tcattcagct ccatct                                 36


<210>  94
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  94
tttggtctca caggaccact tctgcgctcg gcccttccgg ctatct                      46


<210>  95
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  95
tttggtctca cctggtctaa gaactttaaa taatttctac tgttgtagat cggccggtag       60

aaaggaca                                                                68


<210>  96
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  96
tttggtctca agactagagt taagtcaaaa caaaaccc                               38


