                         SEQUENCE LISTING

<110>  Wacker Chemie AG
 
<120>  Verfahren zur Herstellung von Hydroxytyrosol

<130>  HTS

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1632
<212>  DNA
<213>  Ralstonia solanacearum


<220>
<221>  misc_feature
<222>  (1)..(1632)
<223>  rscK60 cds

<400>  1
atgcatcgcc ccccgcttgc ggtcggacgt ttcttttcta cattgaagtg cccgctgcac       60

atcgcatcgc cgggcttccg ggtggctggc tttcctgcgc cgccccacac caacaccaac      120

ggactcatcc aggagaaatc aatggtcgtg cgtagaacgg tgctgaaggc aatcgccgga      180

accagtgtcg ccacggtatt cgcgggcaag ctgaccggtc tctccgctat tgctgccgat      240

gcggccccct tgctggtacg gcgcaacctg cacggcatga agctggacga cccggacctg      300

tcggcctacc gcgagttcgt cagcatcatg aaaagcaaga accagacgca gccgctgagc      360

tggctcggct acgccaacca gcacggctcg ctcaacggtg gcttcaagta ctgcccgcac      420

ggcgattggt acttcctgcc ctggcaccgc ggcttcatgc tgatgtacga acgggccgtg      480

gctgcgctca ccggctacaa gtcattcgcc atgccgtact ggaactggac cgaagaccgc      540

ctgctgcccg aagccttcac cgccaagacc tacaacggca agccgaaccc gctctacgtg      600

cccaaccgga acgatctgac cggtccctat gccctcaccg acgccatcgt cggccagaag      660

gaggtcatgg acaagatcta cgccgaaacc aactttgaag tcttcggcac cagccgctcg      720

gtggatcgct cggtccagcc gccgctggtc cagaacagcc tcgaccccaa atgggtgccg      780

atgggcggcg gcacgcaggg cattctggaa cgcacgccgc acaacaccgt ccacaacaac      840

atcggcgcct tcatgcccac cgccgcctcg ccacgcgacc cggtgttcat gatgcaccac      900

ggcaacatcg accgggtgtg ggccacctgg aatgcgctgg gccgcaagaa ctcgaccgac      960

ccgctgtggc tgggcatgaa gttccccaac aactacattg acccgcaggg ccggtactac     1020

acgcagggcg tgagcgacct gctgagtacc gaggcgctgg gctaccgcta tgacgtcatg     1080

ccgcgcgccg acaacaaggt ggtgaacaac gcccgcgccg agcacctgct ggccctgttc     1140

aagaccggcg acagcgtcaa gctggctgac cacatgaagc tgcgcagcgt gctgaagggg     1200

gaacatccgg ccgccacggc agtcctaccg ctcaacagcg ctgtccagtt cgaggctggc     1260

accgtaacgg gtgccctaag tgccgacgcc gataccggca agaccaccga agtcgtggcc     1320

ctgatcaaga acatcaggat gtccgagaac gtgatcagca tccgggtctt cgtcaacctg     1380

ccggacgcca gtctcgatgt gccggaaacc aacccgcact tcgtcaccac gctcagcttc     1440

ctgacgcacg cggttggaca cgaccaccat gccctgccgt cgaccatggt gaacctgacc     1500

gacacgctga aggcgctcaa tatccgggac gacaatttct cgatcaacct ggttgctgtg     1560

cccaagcccg gcgtcgccgt ggagagcagc ggcagcgtga cacccgagtc gatcgaggtc     1620

gccgtcattt ga                                                         1632


<210>  2
<211>  543
<212>  PRT
<213>  RscK60-Oxidase


<220>
<221>  PEPTIDE
<222>  (1)..(543)
<223>  RscK60-Oxidase

<400>  2

Met His Arg Pro Pro Leu Ala Val Gly Arg Phe Phe Ser Thr Leu Lys 
1               5                   10                  15      


Cys Pro Leu His Ile Ala Ser Pro Gly Phe Arg Val Ala Gly Phe Pro 
            20                  25                  30          


Ala Pro Pro His Thr Asn Thr Asn Gly Leu Ile Gln Glu Lys Ser Met 
        35                  40                  45              


Val Val Arg Arg Thr Val Leu Lys Ala Ile Ala Gly Thr Ser Val Ala 
    50                  55                  60                  


Thr Val Phe Ala Gly Lys Leu Thr Gly Leu Ser Ala Ile Ala Ala Asp 
65                  70                  75                  80  


Ala Ala Pro Leu Leu Val Arg Arg Asn Leu His Gly Met Lys Leu Asp 
                85                  90                  95      


Asp Pro Asp Leu Ser Ala Tyr Arg Glu Phe Val Ser Ile Met Lys Ser 
            100                 105                 110         


Lys Asn Gln Thr Gln Pro Leu Ser Trp Leu Gly Tyr Ala Asn Gln His 
        115                 120                 125             


Gly Ser Leu Asn Gly Gly Phe Lys Tyr Cys Pro His Gly Asp Trp Tyr 
    130                 135                 140                 


Phe Leu Pro Trp His Arg Gly Phe Met Leu Met Tyr Glu Arg Ala Val 
145                 150                 155                 160 


Ala Ala Leu Thr Gly Tyr Lys Ser Phe Ala Met Pro Tyr Trp Asn Trp 
                165                 170                 175     


Thr Glu Asp Arg Leu Leu Pro Glu Ala Phe Thr Ala Lys Thr Tyr Asn 
            180                 185                 190         


Gly Lys Pro Asn Pro Leu Tyr Val Pro Asn Arg Asn Asp Leu Thr Gly 
        195                 200                 205             


Pro Tyr Ala Leu Thr Asp Ala Ile Val Gly Gln Lys Glu Val Met Asp 
    210                 215                 220                 


Lys Ile Tyr Ala Glu Thr Asn Phe Glu Val Phe Gly Thr Ser Arg Ser 
225                 230                 235                 240 


Val Asp Arg Ser Val Gln Pro Pro Leu Val Gln Asn Ser Leu Asp Pro 
                245                 250                 255     


Lys Trp Val Pro Met Gly Gly Gly Thr Gln Gly Ile Leu Glu Arg Thr 
            260                 265                 270         


Pro His Asn Thr Val His Asn Asn Ile Gly Ala Phe Met Pro Thr Ala 
        275                 280                 285             


Ala Ser Pro Arg Asp Pro Val Phe Met Met His His Gly Asn Ile Asp 
    290                 295                 300                 


Arg Val Trp Ala Thr Trp Asn Ala Leu Gly Arg Lys Asn Ser Thr Asp 
305                 310                 315                 320 


Pro Leu Trp Leu Gly Met Lys Phe Pro Asn Asn Tyr Ile Asp Pro Gln 
                325                 330                 335     


Gly Arg Tyr Tyr Thr Gln Gly Val Ser Asp Leu Leu Ser Thr Glu Ala 
            340                 345                 350         


Leu Gly Tyr Arg Tyr Asp Val Met Pro Arg Ala Asp Asn Lys Val Val 
        355                 360                 365             


Asn Asn Ala Arg Ala Glu His Leu Leu Ala Leu Phe Lys Thr Gly Asp 
    370                 375                 380                 


Ser Val Lys Leu Ala Asp His Met Lys Leu Arg Ser Val Leu Lys Gly 
385                 390                 395                 400 


Glu His Pro Ala Ala Thr Ala Val Leu Pro Leu Asn Ser Ala Val Gln 
                405                 410                 415     


Phe Glu Ala Gly Thr Val Thr Gly Ala Leu Ser Ala Asp Ala Asp Thr 
            420                 425                 430         


Gly Lys Thr Thr Glu Val Val Ala Leu Ile Lys Asn Ile Arg Met Ser 
        435                 440                 445             


Glu Asn Val Ile Ser Ile Arg Val Phe Val Asn Leu Pro Asp Ala Ser 
    450                 455                 460                 


Leu Asp Val Pro Glu Thr Asn Pro His Phe Val Thr Thr Leu Ser Phe 
465                 470                 475                 480 


Leu Thr His Ala Val Gly His Asp His His Ala Leu Pro Ser Thr Met 
                485                 490                 495     


Val Asn Leu Thr Asp Thr Leu Lys Ala Leu Asn Ile Arg Asp Asp Asn 
            500                 505                 510         


Phe Ser Ile Asn Leu Val Ala Val Pro Lys Pro Gly Val Ala Val Glu 
        515                 520                 525             


Ser Ser Gly Ser Val Thr Pro Glu Ser Ile Glu Val Ala Val Ile 
    530                 535                 540             


<210>  3
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RscK60-del Oxidase


<220>
<221>  PEPTIDE
<222>  (1)..(496)
<223>  RscK60-del Oxidase

<400>  3

Met Val Val Arg Arg Thr Val Leu Lys Ala Ile Ala Gly Thr Ser Val 
1               5                   10                  15      


Ala Thr Val Phe Ala Gly Lys Leu Thr Gly Leu Ser Ala Val Ala Ala 
            20                  25                  30          


Asp Ala Ala Pro Leu Arg Val Arg Arg Asn Leu His Gly Met Lys Met 
        35                  40                  45              


Asp Asp Pro Asp Leu Ser Ala Tyr Arg Glu Phe Val Gly Ile Met Lys 
    50                  55                  60                  


Gly Lys Asp Gln Thr Gln Ala Leu Ser Trp Leu Gly Phe Ala Asn Gln 
65                  70                  75                  80  


His Gly Thr Leu Asn Gly Gly Tyr Lys Tyr Cys Pro His Gly Asp Trp 
                85                  90                  95      


Tyr Phe Leu Pro Trp His Arg Gly Phe Val Leu Met Tyr Glu Arg Ala 
            100                 105                 110         


Val Ala Ala Leu Thr Gly Tyr Lys Thr Phe Ala Met Pro Tyr Trp Asn 
        115                 120                 125             


Trp Thr Glu Asp Arg Leu Leu Pro Glu Ala Phe Thr Ala Lys Thr Tyr 
    130                 135                 140                 


Asn Gly Lys Thr Asn Pro Leu Tyr Val Pro Asn Arg Asn Glu Leu Thr 
145                 150                 155                 160 


Gly Pro Tyr Ala Leu Thr Asp Ala Ile Val Gly Gln Lys Glu Val Met 
                165                 170                 175     


Asp Lys Ile Tyr Ala Glu Thr Asn Phe Glu Val Phe Gly Thr Ser Arg 
            180                 185                 190         


Ser Val Asp Arg Ser Val Arg Pro Pro Leu Val Gln Asn Ser Leu Asp 
        195                 200                 205             


Pro Lys Trp Val Pro Met Gly Gly Gly Asn Gln Gly Ile Leu Glu Arg 
    210                 215                 220                 


Thr Pro His Asn Thr Val His Asn Asn Ile Gly Ala Phe Met Pro Thr 
225                 230                 235                 240 


Ala Ala Ser Pro Arg Asp Pro Val Phe Met Met His His Gly Asn Ile 
                245                 250                 255     


Asp Arg Val Trp Ala Thr Trp Asn Ala Leu Gly Arg Lys Asn Ser Thr 
            260                 265                 270         


Asp Pro Leu Trp Leu Gly Met Lys Phe Pro Asn Asn Tyr Ile Asp Pro 
        275                 280                 285             


Gln Gly Arg Tyr Tyr Thr Gln Gly Val Ser Asp Leu Leu Ser Thr Glu 
    290                 295                 300                 


Ala Leu Gly Tyr Arg Tyr Asp Val Met Pro Arg Ala Asp Asn Lys Val 
305                 310                 315                 320 


Val Asn Asn Ala Arg Ala Glu His Leu Leu Ala Leu Phe Lys Thr Gly 
                325                 330                 335     


Asp Ser Val Lys Leu Ala Asp His Ile Arg Leu Arg Ser Val Leu Lys 
            340                 345                 350         


Gly Glu His Pro Val Ala Thr Ala Val Glu Pro Leu Asn Ser Ala Val 
        355                 360                 365             


Gln Phe Glu Ala Gly Thr Val Thr Gly Ala Leu Gly Ala Asp Val Gly 
    370                 375                 380                 


Thr Gly Ser Thr Thr Glu Val Val Ala Leu Ile Lys Asn Ile Arg Ile 
385                 390                 395                 400 


Pro Tyr Asn Val Ile Ser Ile Arg Val Phe Val Asn Leu Pro Asn Ala 
                405                 410                 415     


Asn Leu Asp Val Pro Glu Thr Asp Pro His Phe Val Thr Ser Leu Ser 
            420                 425                 430         


Phe Leu Thr His Ala Ala Gly His Asp His His Ala Leu Pro Ser Thr 
        435                 440                 445             


Met Val Asn Leu Thr Asp Thr Leu Lys Ala Leu Asn Ile Arg Asp Asp 
    450                 455                 460                 


Asn Phe Ser Ile Asn Leu Val Ala Val Pro Gln Pro Gly Val Ala Val 
465                 470                 475                 480 


Glu Ser Ser Gly Gly Val Thr Pro Glu Ser Ile Glu Val Ala Val Ile 
                485                 490                 495     


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer forward


<220>
<221>  primer_bind
<222>  (1)..(33)
<223>  rsck60-1f

<400>  4
ttaagaattc atgcatcgcc ccccgcttgc ggt                                    33


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer reverse


<220>
<221>  primer_bind
<222>  (1)..(34)
<223>  rsck60-2r

<400>  5
atataagctt tcaaatgacg gcgacctcga tcga                                   34


<210>  6
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer forward


<220>
<221>  primer_bind
<222>  (1)..(34)
<223>  rsck60-3f

<400>  6
ttaagaattc atggtcgtgc gtagaacggt gctg                                   34


