                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  ASSEMBLED THREE-DIMENSIONAL CULTURES OF HUMAN NEURONS AND GLIA 
       AND THEIR USE

<130>  UCSF-639WO

<150>  63/215,179
<151>  2021-06-25

<150>  63/154,305
<151>  2021-02-26

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GRN single guide


<220>
<221>  source
<222>  (1)..(20)
<223>  sequence is synthesized

<400>  1
gtgcccaagg accgcggagt                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  no-known target scramble control


<220>
<221>  source
<222>  (1)..(20)
<223>  sequence is synthesized

<400>  2
ggactaagcg caagcaccta                                                   20


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GRN_F primer


<220>
<221>  source
<222>  (1)..(21)
<223>  sequence is synthesized

<400>  3
tccagagtaa gtgcctctcc a                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GRN_R primer


<220>
<221>  source
<222>  (1)..(21)
<223>  sequence is synthesized

<400>  4
tcacctccat gtcacatttc a                                                 21


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH_F primer


<220>
<221>  source
<222>  (1)..(19)
<223>  sequence is synthesized

<400>  5
atggggaagg tgaaggtcg                                                    19


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH_R primer


<220>
<221>  source
<222>  (1)..(22)
<223>  sequence is synthesized

<400>  6
ggggtcattg atggcaacaa ta                                                22


