                         SEQUENCE LISTING

<110>  Pluristyx, Inc.
 
<120>  ENGINEERED EXPRESSION CONSTRUCTS TO INCREASE PROTEIN EXPRESSION 
       FROM SYNTHETIC RIBONUCLEIC ACID (RNA)

<130>  P183-0010PCT

<150>  US 63/153,877
<151>  2021-02-25

<160>  83    

<170>  PatentIn version 3.5

<210>  1

<400>  1
000

<210>  2
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' UTR Construct with "A" Kozak Sequence (no start codon)

<400>  2
gggagacaua cucagccacc                                                   20


<210>  3
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' UTR Construct with "G" Kozak Sequence (no start codon)

<400>  3
gggagacaua cucagccgcc                                                   20


<210>  4
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  4
nnnauaccuc nnnnnnnnnn nnnnngagg                                         29


<210>  5
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  5
nnnaaaccuc nnnnnnnnnn nnnnngagg                                         29


<210>  6
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  6
nnnauaggag nnnnnnnnnn nnnnncucc                                         29


<210>  7
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  7
nnnaaaggag nnnnnnnnnn nnnnncucc                                         29


<210>  8
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (10)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  8
nnnauagagn nnnnnnnnnn nnnnccuc                                          28


<210>  9
<211>  29
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n can be present or absent

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n can be present or absent

<400>  9
nnnaaagagg nnnnnnnnnn nnnnnccuc                                         29


<210>  10
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  10
ugcauaccuc uaacggucuu gagg                                              24


<210>  11
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  11
ugcauaggag uaacggucuu cucc                                              24


<210>  12
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  12
ugcaaaggag uaacggucuu cucc                                              24


<210>  13
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  13
uaaugcauac cucuaacggu cuugagg                                           27


<210>  14
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  14
ugaugcauac cucuaacggu cuugagg                                           27


<210>  15
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  15
uagugcauac cucuaacggu cuugagg                                           27


<210>  16
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  16
uaaugcauag agguaacggu cuuccuc                                           27


<210>  17
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  17
ugaugcauag agguaacggu cuuccuc                                           27


<210>  18
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  18
uagugcauag agguaacggu cuuccuc                                           27


<210>  19
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  19
uaaugcaaag agguaacggu cuuccuc                                           27


<210>  20
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  20
ugaugcaaag agguaacggu cuuccuc                                           27


<210>  21
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'UTR construct

<400>  21
uagugcaaag agguaacggu cuuccuc                                           27


<210>  22

<400>  22
000

<210>  23

<400>  23
000

<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer (No UTR)

<400>  24
gactcctaat acgactcact atagggagaa tg                                     32


<210>  25
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer (Kozak)

<400>  25
gactcctaat acgactcact atagggagag ccaccatg                               38


<210>  26
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer (5'UTR)

<400>  26
gactcctaat acgactcact atagggagac atactcagcc accatg                      46


<210>  27
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse (3'UTR-A)

<400>  27
tttttttttt tttttttttt tttttttttt tttttttttt cctcaagacc gttagaggta       60

tgca                                                                    64


<210>  28
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse (3'UTR-B)

<400>  28
tttttttttt tttttttttt tttttttttt tttttttttt ggagaagacc gttactccta       60

tgca                                                                    64


<210>  29
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse (3'UTR-C)

<400>  29
tttttttttt tttttttttt tttttttttt tttttttttt ggagaagacc gttactcctt       60

tgca                                                                    64


<210>  30
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak sequence

<400>  30
gccrccatgg                                                              10


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter and hexamer

<400>  31
taatacgact cactataggg aga                                               23


<210>  32

<400>  32
000

<210>  33

<400>  33
000

<210>  34
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  34
uaacggucuu                                                              10


<210>  35

<400>  35
000

<210>  36

<400>  36
000

<210>  37

<400>  37
000

<210>  38
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' UTR Construct with "A" Kozak Sequence (with start codon)

<400>  38
gggagacaua cucagccacc aug                                               23


<210>  39
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' UTR Construct with "G" Kozak Sequence (with start codon)

<400>  39
gggagacaua cucagccgcc aug                                               23


<210>  40

<400>  40
000

<210>  41

<400>  41
000

<210>  42

<400>  42
000

<210>  43

<400>  43
000

<210>  44

<400>  44
000

<210>  45
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA double-helix sequence

<400>  45
taatacgact cactatag                                                     18


<210>  46

<400>  46
000

<210>  47

<400>  47
000

<210>  48
<211>  32
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  48

Val Gly Phe Pro Val Thr Pro Gln Val Pro Leu Arg Pro Met Thr Tyr 
1               5                   10                  15      


Lys Ala Ala Val Asp Leu Ser His Phe Leu Lys Glu Lys Gly Gly Leu 
            20                  25                  30          


<210>  49
<211>  30
<212>  PRT
<213>  DNA double-helix sequence

<400>  49

His Thr Gln Gly Tyr Phe Pro Asp Trp Gln Asn Tyr Thr Pro Gly Pro 
1               5                   10                  15      


Gly Val Arg Tyr Pro Leu Thr Phe Gly Trp Leu Tyr Lys Leu 
            20                  25                  30  


<210>  50
<211>  19
<212>  PRT
<213>  DNA double-helix sequence

<400>  50

Glu Lys Ile Arg Leu Arg Pro Gly Gly Lys Lys Lys Tyr Lys Leu Lys 
1               5                   10                  15      


His Ile Val 
            


<210>  51
<211>  32
<212>  PRT
<213>  DNA double-helix sequence

<400>  51

Asn Pro Pro Ile Pro Val Gly Glu Ile Tyr Lys Arg Trp Ile Ile Leu 
1               5                   10                  15      


Gly Leu Asn Lys Ile Val Arg Met Tyr Ser Pro Thr Ser Ile Leu Asp 
            20                  25                  30          


<210>  52
<211>  31
<212>  PRT
<213>  DNA double-helix sequence

<400>  52

Ala Ile Phe Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys 
1               5                   10                  15      


Gln Asn Pro Asp Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr 
            20                  25                  30      


<210>  53
<211>  20
<212>  PRT
<213>  Plasmodium falciparum

<400>  53

Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 
1               5                   10                  15      


Asn Ala Asn Pro 
            20  


<210>  54
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E protein Domain III

<400>  54

Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Thr Val Thr 
1               5                   10                  15      


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
            20                  25                  30          


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
        35                  40                  45              


Ala Asn Pro Val Ile Thr Glu Gly Thr Glu Asn Ser Lys Met Met Leu 
    50                  55                  60                  


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
65                  70                  75                  80  


Glu 
    


<210>  55
<211>  827
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFP

<400>  55
gactcctaat acgactcact atagggagac atactcagcc accatggtga gcaagggcga       60

ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca      120

caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa      180

gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac      240

ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa      300

gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa      360

ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct      420

gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta      480

caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt      540

caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa      600

cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc      660

cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac      720

cgccgccggg atcactctcg gcatggacga gctgtacaag taatgcatac ctctaacggt      780

cttgaggaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa                    827


<210>  56
<211>  804
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  EGFP

<400>  56
gggagacaua cucagccacc auggugagca agggcgagga gcuguucacc gggguggugc       60

ccauccuggu cgagcuggac ggcgacguaa acggccacaa guucagcgug uccggcgagg      120

gcgagggcga ugccaccuac ggcaagcuga cccugaaguu caucugcacc accggcaagc      180

ugcccgugcc cuggcccacc cucgugacca cccugaccua cggcgugcag ugcuucagcc      240

gcuaccccga ccacaugaag cagcacgacu ucuucaaguc cgccaugccc gaaggcuacg      300

uccaggagcg caccaucuuc uucaaggacg acggcaacua caagacccgc gccgagguga      360

aguucgaggg cgacacccug gugaaccgca ucgagcugaa gggcaucgac uucaaggagg      420

acggcaacau ccuggggcac aagcuggagu acaacuacaa cagccacaac gucuauauca      480

uggccgacaa gcagaagaac ggcaucaagg ugaacuucaa gauccgccac aacaucgagg      540

acggcagcgu gcagcucgcc gaccacuacc agcagaacac ccccaucggc gacggccccg      600

ugcugcugcc cgacaaccac uaccugagca cccaguccgc ccugagcaaa gaccccaacg      660

agaagcgcga ucacaugguc cugcuggagu ucgugaccgc cgccgggauc acucucggca      720

uggacgagcu guacaaguaa ugcauaccuc uaacggucuu gaggaaaaaa aaaaaaaaaa      780

aaaaaaaaaa aaaaaaaaaa aaaa                                             804


<210>  57
<211>  1190
<212>  DNA
<213>  Homo sapiens

<400>  57
gactcctaat acgactcact atagggagac atactcagcc accatggcgg gacacctggc       60

ttcggatttc gccttctcgc cccctccagg tggtggaggt gatgggccag gggggccgga      120

gccgggctgg gttgatcctc ggacctggct aagcttccaa ggccctcctg gagggccagg      180

aatcgggccg ggggttgggc caggctctga ggtgtggggg attcccccat gccccccgcc      240

gtatgagttc tgtgggggga tggcgtactg tgggccccag gttggagtgg ggctagtgcc      300

ccaaggcggc ttggagacct ctcagcctga gggcgaagca ggagtcgggg tggagagcaa      360

ctccgatggg gcctccccgg agccctgcac cgtcacccct ggtgccgtga agctggagaa      420

ggagaagctg gagcaaaacc cggaggagtc ccaggacatc aaagctctgc agaaagaact      480

cgagcaattt gccaagctcc tgaagcagaa gaggatcacc ctgggatata cacaggccga      540

tgtggggctc accctggggg ttctatttgg gaaggtattc agccaaacga ccatctgccg      600

ctttgaggct ctgcagctta gcttcaagaa catgtgtaag ctgcggccct tgctgcagaa      660

gtgggtggag gaagctgaca acaatgaaaa tcttcaggag atatgcaaag cagaaaccct      720

cgtgcaggcc cgaaagagaa agcgaaccag tatcgagaac cgagtgagag gcaacctgga      780

gaatttgttc ctgcagtgcc cgaaacccac actgcagcag atcagccaca tcgcccagca      840

gcttgggctc gagaaggatg tggtccgagt gtggttctgt aaccggcgcc agaagggcaa      900

gcgatcaagc agcgactatg cacaacgaga ggattttgag gctgctgggt ctcctttctc      960

agggggacca gtgtcctttc ctctggcccc agggccccat tttggtaccc caggctatgg     1020

gagccctcac ttcactgcac tgtactcctc ggtccctttc cctgaggggg aagcctttcc     1080

ccctgtctcc gtcaccactc tgggctctcc catgcattca aactgatgca tacctctaac     1140

ggtcttgagg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                1190


<210>  58
<211>  1167
<212>  RNA
<213>  Homo sapiens

<400>  58
gggagacaua cucagccacc auggcgggac accuggcuuc ggauuucgcc uucucgcccc       60

cuccaggugg uggaggugau gggccagggg ggccggagcc gggcuggguu gauccucgga      120

ccuggcuaag cuuccaaggc ccuccuggag ggccaggaau cgggccgggg guugggccag      180

gcucugaggu gugggggauu cccccaugcc ccccgccgua ugaguucugu ggggggaugg      240

cguacugugg gccccagguu ggaguggggc uagugcccca aggcggcuug gagaccucuc      300

agccugaggg cgaagcagga gucggggugg agagcaacuc cgauggggcc uccccggagc      360

ccugcaccgu caccccuggu gccgugaagc uggagaagga gaagcuggag caaaacccgg      420

aggaguccca ggacaucaaa gcucugcaga aagaacucga gcaauuugcc aagcuccuga      480

agcagaagag gaucacccug ggauauacac aggccgaugu ggggcucacc cuggggguuc      540

uauuugggaa gguauucagc caaacgacca ucugccgcuu ugaggcucug cagcuuagcu      600

ucaagaacau guguaagcug cggcccuugc ugcagaagug gguggaggaa gcugacaaca      660

augaaaaucu ucaggagaua ugcaaagcag aaacccucgu gcaggcccga aagagaaagc      720

gaaccaguau cgagaaccga gugagaggca accuggagaa uuuguuccug cagugcccga      780

aacccacacu gcagcagauc agccacaucg cccagcagcu ugggcucgag aaggaugugg      840

uccgagugug guucuguaac cggcgccaga agggcaagcg aucaagcagc gacuaugcac      900

aacgagagga uuuugaggcu gcugggucuc cuuucucagg gggaccagug uccuuuccuc      960

uggccccagg gccccauuuu gguaccccag gcuaugggag cccucacuuc acugcacugu     1020

acuccucggu cccuuucccu gagggggaag ccuuuccccc ugucuccguc accacucugg     1080

gcucucccau gcauucaaac ugaugcauac cucuaacggu cuugaggaaa aaaaaaaaaa     1140

aaaaaaaaaa aaaaaaaaaa aaaaaaa                                         1167


<210>  59
<211>  569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL2 Complete DNA Sequence (with Riboz UTRs and PolyA tail)

<400>  59
gactcctaat acgactcact atagggagac atactcagcc accatgtaca ggatgcaact       60

cctgtcttgc attgcactaa gtcttgcact tgtcacaaac agtgcaccta cttcaagttc      120

tacaaagaaa acacagctac aactggagca tttactgctg gatttacaga tgattttgaa      180

tggaattaat aattacaaga atcccaaact caccaggatg ctcacattta agttttacat      240

gcccaagaag gccacagaac tgaaacatct tcagtgtcta gaagaagaac tcaaacctct      300

ggaggaagtg ctaaatttag ctcaaagcaa aaactttcac ttaagaccca gggacttaat      360

cagcaatatc aacgtaatag ttctggaact aaagggatct gaaacaacat tcatgtgtga      420

atatgctgat gagacagcaa ccattgtaga atttctgaac agatggatta ccttttgtca      480

aagcatcatc tcaacactga cttgatgcat acctctaacg gtcttgagga aaaaaaaaaa      540

aaaaaaaaaa aaaaaaaaaa aaaaaaaaa                                        569


<210>  60
<211>  546
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Complete RNA Sequence (with Riboz UTRs and PolyA tail)

<400>  60
gggagacaua cucagccacc auguacagga ugcaacuccu gucuugcauu gcacuaaguc       60

uugcacuugu cacaaacagu gcaccuacuu caaguucuac aaagaaaaca cagcuacaac      120

uggagcauuu acugcuggau uuacagauga uuuugaaugg aauuaauaau uacaagaauc      180

ccaaacucac caggaugcuc acauuuaagu uuuacaugcc caagaaggcc acagaacuga      240

aacaucuuca gugucuagaa gaagaacuca aaccucugga ggaagugcua aauuuagcuc      300

aaagcaaaaa cuuucacuua agacccaggg acuuaaucag caauaucaac guaauaguuc      360

uggaacuaaa gggaucugaa acaacauuca ugugugaaua ugcugaugag acagcaacca      420

uuguagaauu ucugaacaga uggauuaccu uuugucaaag caucaucuca acacugacuu      480

gaugcauacc ucuaacgguc uugaggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      540

aaaaaa                                                                 546


<210>  61

<400>  61
000

<210>  62

<400>  62
000

<210>  63
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' UTR construct

<400>  63
gggagagcca ccaug                                                        15


<210>  64
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  64
gaugccccau ucacgaguag uggguauu                                          28


<210>  65
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  65
ggcacccugc gcaggugaug caggugcc                                          28


<210>  66
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  66
guucgcucgg ucaggagagc ugacggac                                          28


<210>  67
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  67
ucuuacagug gcaugugacc guuuaagg                                          28


<210>  68
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  68
cgcggcgcau gcacgugaca ugccugcg                                          28


<210>  69
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  69
cggucccgug gcaagagucu auggauug                                          28


<210>  70
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  70
auguucggcu ccaagagcga guugauau                                          28


<210>  71
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  71
cgauucgggc acaugugcug ucugauug                                          28


<210>  72
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  72
guauucugau gcacgugcca ucaaguac                                          28


<210>  73
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  73
uugagcagga ucaagugcau ucuuucaa                                          28


<210>  74
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  74
rryryyyyry yyryrrryrr yrrryryy                                          28


<210>  75
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  75
rryryyyyry ryrrryrryr yrrryryy                                          28


<210>  76
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  76
ryyyryyyrr yyrrrrrrry yrryrrry                                          28


<210>  77
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  77
yyyyryrryr ryryryrryy ryyyrrrr                                          28


<210>  78
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  78
yryrryryry ryryryrryr yryyyryr                                          28


<210>  79
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  79
yrryyyyryr ryrrrrryyy ryrrryyr                                          28


<210>  80
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  80
ryryyyrryy yyrrrrryrr ryyrryry                                          28


<210>  81
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  81
yrryyyrrry ryryryryyr yyyrryyr                                          28


<210>  82
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  82
ryryyyyrry ryryryryyr yyrrryry                                          28


<210>  83
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Loop segment of stem loop

<400>  83
yyrrryrrrr yyrrryryry yyyyyyrr                                          28


