                         SEQUENCE LISTING

<110>  Alliance for Sustainable Energy, LLC.
 
<120>  ENGINEERED THERMOSTABLE CARBONIC ANHYDRASE ENZYMES

<130>  NREL PCT/20-137

<150>  63/151,506
<151>  2021-02-19

<150>  63/174,337
<151>  2021-04-13

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3

<400>  1

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Gly 
            20                  25                  30          


Trp Ser Tyr His Gly Glu His Gly Pro Glu His Trp Gly Asp Leu Lys 
        35                  40                  45              


Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro Val Asp 
    50                  55                  60                  


Ile Asn Arg Ile Val Asp Ala Lys Leu Lys Pro Ile Lys Ile Glu Tyr 
65                  70                  75                  80  


Arg Ala Gly Ala Thr Lys Val Leu Asn Asn Gly His Thr Ile Lys Val 
                85                  90                  95      


Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val Asp Gly Ile Lys Phe Glu 
            100                 105                 110         


Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Lys Leu Lys Gly 
        115                 120                 125             


Gln His Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys His Gly 
    130                 135                 140                 


Asn Leu Ala Val Ile Gly Val Phe Phe Lys Glu Gly Arg Glu Asn Pro 
145                 150                 155                 160 


Ile Leu Glu Lys Ile Trp Lys Val Met Pro Glu Asn Ala Gly Glu Glu 
                165                 170                 175     


Val Lys Leu Ala His Lys Ile Asn Ala Glu Asp Leu Leu Pro Lys Asp 
            180                 185                 190         


Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys Ser 
        195                 200                 205             


Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Met Ser Lys 
    210                 215                 220                 


Glu Gln Ile Glu Lys Phe Arg Lys Ile Met Gly Gly Asp Thr Asn Arg 
225                 230                 235                 240 


Pro Val Gln Pro Leu Asn Ala Arg Met Ile Met Glu Lys 
                245                 250             


<210>  2
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3

<400>  2
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggaggatgga gctatcatgg cgaacatgga      120

cctgaacatt ggggtgacct gaaagacgaa tatattatgt gcaaaatcgg caaaaatcaa      180

tcaccggttg atattaacag aatcgtggat gcaaaactta aaccgatcaa aatcgaatat      240

cgcgcaggag cgacaaaagt cctgaacaac ggccatacaa tcaaagtttc ttatgaaccg      300

ggatcatata ttgttgtgga tggcatcaaa tttgaattaa aacaatttca ttttcatgca      360

ccgagcgaac ataaactgaa aggacagcat tatccgtttg aagctcattt tgttcatgcc      420

gataaacatg gcaatctggc tgtcatcgga gttttcttta aagaaggcag agaaaacccg      480

attcttgaaa aaatctggaa agtgatgccg gaaaatgccg gcgaagaagt caaattagca      540

cataaaatca acgcggaaga tttactgccg aaagatagag attattatcg ctattcagga      600

agcctgacaa caccgccgtg cagcgaaggc gtgagatgga tcgtcatgga agaagaaatg      660

gaaatgtcta aagaacagat cgaaaaattt cgcaaaatca tgggaggcga tacgaaccgt      720

cctgtgcagc cgttgaatgc gagaatgatt atggaaaaat aa                         762


<210>  3
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut1

<400>  3

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Gly 
            20                  25                  30          


Trp Ser Tyr His Gly Glu His Gly Pro Glu His Trp Gly Asp Leu Lys 
        35                  40                  45              


Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro Val Asp 
    50                  55                  60                  


Ile Asn Arg Ile Val Asp Ala Lys Leu Lys Pro Ile Lys Ile Glu Tyr 
65                  70                  75                  80  


Arg Ala Gly Ala Thr Lys Val Leu Asn Asn Gly His Thr Ile Lys Val 
                85                  90                  95      


Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val Asp Gly Ile Lys Phe Glu 
            100                 105                 110         


Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Lys Leu Lys Gly 
        115                 120                 125             


Gln His Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys His Gly 
    130                 135                 140                 


Asn Leu Ala Val Ile Gly Val Phe Phe Lys Glu Gly Arg Glu Asn Pro 
145                 150                 155                 160 


Ile Leu Glu Lys Ile Trp Lys Val Met Pro Glu Asn Ala Gly Glu Glu 
                165                 170                 175     


Val Lys Leu Ala His Lys Ile Asn Ala Glu Asp Leu Leu Pro Lys Asp 
            180                 185                 190         


Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys Ser 
        195                 200                 205             


Glu Cys Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Met Ser Lys 
    210                 215                 220                 


Glu Gln Ile Glu Lys Phe Arg Lys Ile Met Gly Gly Asp Thr Asn Arg 
225                 230                 235                 240 


Pro Val Gln Pro Leu Cys Ala Arg Met Ile Met Glu Lys 
                245                 250             


<210>  4
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut1

<400>  4
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggaggatgga gctatcatgg cgaacatgga      120

cctgaacatt ggggtgacct gaaagacgaa tatattatgt gcaaaatcgg caaaaatcaa      180

tcaccggttg atattaacag aatcgtggat gcaaaactta aaccgatcaa aatcgaatat      240

cgcgcaggag cgacaaaagt cctgaacaac ggccatacaa tcaaagtttc ttatgaaccg      300

ggatcatata ttgttgtgga tggcatcaaa tttgaattaa aacaatttca ttttcatgca      360

ccgagcgaac ataaactgaa aggacagcat tatccgtttg aagctcattt tgttcatgcc      420

gataaacatg gcaatctggc tgtcatcgga gttttcttta aagaaggcag agaaaacccg      480

attcttgaaa aaatctggaa agtgatgccg gaaaatgccg gcgaagaagt caaattagca      540

cataaaatca acgcggaaga tttactgccg aaagatagag attattatcg ctattcagga      600

agcctgacaa caccgccgtg cagcgaatgc gtgagatgga tcgtcatgga agaagaaatg      660

gaaatgtcta aagaacagat cgaaaaattt cgcaaaatca tgggaggcga tacgaaccgt      720

cctgtgcagc cgttgtgtgc gagaatgatt atggaaaaat aa                         762


<210>  5
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut2

<400>  5

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Gly 
            20                  25                  30          


Trp Ser Tyr His Gly Glu His Gly Pro Glu His Trp Gly Asp Leu Lys 
        35                  40                  45              


Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro Val Asp 
    50                  55                  60                  


Ile Asn Arg Ile Val Asp Cys Lys Leu Lys Pro Ile Lys Ile Glu Tyr 
65                  70                  75                  80  


Arg Ala Gly Ala Thr Lys Val Leu Asn Asn Gly His Thr Ile Lys Val 
                85                  90                  95      


Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val Asp Gly Ile Lys Phe Glu 
            100                 105                 110         


Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Lys Leu Lys Gly 
        115                 120                 125             


Gln His Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys His Gly 
    130                 135                 140                 


Asn Leu Ala Val Ile Gly Val Phe Phe Lys Glu Gly Arg Glu Asn Pro 
145                 150                 155                 160 


Ile Leu Glu Lys Ile Trp Lys Val Met Pro Glu Asn Ala Gly Glu Glu 
                165                 170                 175     


Val Lys Leu Ala His Lys Ile Asn Ala Glu Asp Leu Leu Pro Lys Asp 
            180                 185                 190         


Arg Asp Tyr Tyr Arg Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys Ser 
        195                 200                 205             


Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Met Ser Lys 
    210                 215                 220                 


Glu Gln Ile Glu Lys Phe Arg Lys Ile Met Gly Gly Asp Thr Asn Arg 
225                 230                 235                 240 


Pro Val Gln Pro Leu Asn Ala Arg Met Ile Met Glu Lys 
                245                 250             


<210>  6
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut2

<400>  6
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggaggatgga gctatcatgg cgaacatgga      120

cctgaacatt ggggtgacct gaaagacgaa tatattatgt gcaaaatcgg caaaaatcaa      180

tcaccggttg atattaacag aatcgtggat tgtaaactta aaccgatcaa aatcgaatat      240

cgcgcaggag cgacaaaagt cctgaacaac ggccatacaa tcaaagtttc ttatgaaccg      300

ggatcatata ttgttgtgga tggcatcaaa tttgaattaa aacaatttca ttttcatgca      360

ccgagcgaac ataaactgaa aggacagcat tatccgtttg aagctcattt tgttcatgcc      420

gataaacatg gcaatctggc tgtcatcgga gttttcttta aagaaggcag agaaaacccg      480

attcttgaaa aaatctggaa agtgatgccg gaaaatgccg gcgaagaagt caaattagca      540

cataaaatca acgcggaaga tttactgccg aaagatagag attattatcg ctattcagga      600

agcctgacaa caccgccgtg cagcgaaggc gtgagatgga tcgtcatgga agaagaaatg      660

gaaatgtcta aagaacagat cgaaaaattt cgcaaaatca tgggaggcga tacgaaccgt      720

cctgtgcagc cgttgaatgc gagaatgatt atggaaaaat aa                         762


<210>  7
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut3

<400>  7

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Gly 
            20                  25                  30          


Trp Ser Tyr His Gly Glu His Gly Pro Glu His Trp Gly Asp Leu Lys 
        35                  40                  45              


Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro Val Asp 
    50                  55                  60                  


Ile Asn Arg Ile Val Asp Ala Lys Leu Lys Pro Ile Lys Ile Glu Tyr 
65                  70                  75                  80  


Arg Ala Gly Ala Thr Lys Val Leu Asn Asn Gly His Thr Ile Lys Val 
                85                  90                  95      


Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val Asp Gly Ile Lys Phe Glu 
            100                 105                 110         


Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Lys Leu Lys Gly 
        115                 120                 125             


Gln His Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys His Gly 
    130                 135                 140                 


Asn Leu Ala Val Ile Gly Val Phe Phe Lys Glu Gly Arg Glu Asn Pro 
145                 150                 155                 160 


Ile Leu Glu Lys Ile Trp Lys Val Met Pro Glu Asn Ala Gly Glu Glu 
                165                 170                 175     


Val Lys Leu Ala His Lys Ile Asn Ala Glu Asp Leu Leu Pro Lys Asp 
            180                 185                 190         


Arg Asp Tyr Tyr Arg Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys Ser 
        195                 200                 205             


Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Met Ser Lys 
    210                 215                 220                 


Glu Gln Ile Glu Lys Phe Arg Lys Ile Met Gly Gly Asp Thr Asn Arg 
225                 230                 235                 240 


Pro Val Gln Pro Leu Cys Ala Arg Met Ile Met Glu Lys 
                245                 250             


<210>  8
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut3

<400>  8
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggaggatgga gctatcatgg cgaacatgga      120

cctgaacatt ggggtgacct gaaagacgaa tatattatgt gcaaaatcgg caaaaatcaa      180

tcaccggttg atattaacag aatcgtggat gcaaaactta aaccgatcaa aatcgaatat      240

cgcgcaggag cgacaaaagt cctgaacaac ggccatacaa tcaaagtttc ttatgaaccg      300

ggatcatata ttgttgtgga tggcatcaaa tttgaattaa aacaatttca ttttcatgca      360

ccgagcgaac ataaactgaa aggacagcat tatccgtttg aagctcattt tgttcatgcc      420

gataaacatg gcaatctggc tgtcatcgga gttttcttta aagaaggcag agaaaacccg      480

attcttgaaa aaatctggaa agtgatgccg gaaaatgccg gcgaagaagt caaattagca      540

cataaaatca acgcggaaga tttactgccg aaagatagag attattatcg ctattgtgga      600

agcctgacaa caccgccgtg cagcgaaggc gtgagatgga tcgtcatgga agaagaaatg      660

gaaatgtcta aagaacagat cgaaaaattt cgcaaaatca tgggaggcga tacgaaccgt      720

cctgtgcagc cgttgtgtgc gagaatgatt atggaaaaat aa                         762


<210>  9
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut23

<400>  9

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Gly 
            20                  25                  30          


Trp Ser Tyr His Gly Glu His Gly Pro Glu His Trp Gly Asp Leu Lys 
        35                  40                  45              


Asp Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro Val Asp 
    50                  55                  60                  


Ile Asn Arg Ile Val Asp Cys Lys Leu Lys Pro Ile Lys Ile Glu Tyr 
65                  70                  75                  80  


Arg Ala Gly Ala Thr Lys Val Leu Asn Asn Gly His Thr Ile Lys Val 
                85                  90                  95      


Ser Tyr Glu Pro Gly Ser Tyr Ile Val Val Asp Gly Ile Lys Phe Glu 
            100                 105                 110         


Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Lys Leu Lys Gly 
        115                 120                 125             


Gln His Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys His Gly 
    130                 135                 140                 


Asn Leu Ala Val Ile Gly Val Phe Phe Lys Glu Gly Arg Glu Asn Pro 
145                 150                 155                 160 


Ile Leu Glu Lys Ile Trp Lys Val Met Pro Glu Asn Ala Gly Glu Glu 
                165                 170                 175     


Val Lys Leu Ala His Lys Ile Asn Ala Glu Asp Leu Leu Pro Lys Asp 
            180                 185                 190         


Arg Asp Tyr Tyr Arg Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys Ser 
        195                 200                 205             


Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Met Ser Lys 
    210                 215                 220                 


Glu Gln Ile Glu Lys Phe Arg Lys Ile Met Gly Gly Asp Thr Asn Arg 
225                 230                 235                 240 


Pro Val Gln Pro Leu Asn Cys Arg Met Ile Met Glu Lys 
                245                 250             


<210>  10
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-PmaCA-CA3mut23

<400>  10
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggaggatgga gctatcatgg cgaacatgga      120

cctgaacatt ggggtgacct gaaagacgaa tatattatgt gcaaaatcgg caaaaatcaa      180

tcaccggttg atattaacag aatcgtggat tgtaaactta aaccgatcaa aatcgaatat      240

cgcgcaggag cgacaaaagt cctgaacaac ggccatacaa tcaaagtttc ttatgaaccg      300

ggatcatata ttgttgtgga tggcatcaaa tttgaattaa aacaatttca ttttcatgca      360

ccgagcgaac ataaactgaa aggacagcat tatccgtttg aagctcattt tgttcatgcc      420

gataaacatg gcaatctggc tgtcatcgga gttttcttta aagaaggcag agaaaacccg      480

attcttgaaa aaatctggaa agtgatgccg gaaaatgccg gcgaagaagt caaattagca      540

cataaaatca acgcggaaga tttactgccg aaagatagag attattatcg ctattgtgga      600

agcctgacaa caccgccgtg cagcgaaggc gtgagatgga tcgtcatgga agaagaaatg      660

gaaatgtcta aagaacagat cgaaaaattt cgcaaaatca tgggaggcga tacgaaccgt      720

cctgtgcagc cgttgtgtgc gagaatgatt atggaaaaat aa                         762


<210>  11
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4

<400>  11

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Val 
            20                  25                  30          


Gly His Trp Ser Tyr His Gly Glu Thr Gly Pro Gln His Trp Gly Asp 
        35                  40                  45              


Leu Lys Asn Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro 
    50                  55                  60                  


Val Asp Ile Ser Arg Ile Val Glu Ala Glu Leu Glu Lys Ile Lys Ile 
65                  70                  75                  80  


Asn Tyr Ser Ser Gly Gly Ser Ser Ile Thr Asn Asn Gly His Thr Ile 
                85                  90                  95      


Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Ile Val Asp Gly Ile Arg 
            100                 105                 110         


Phe Glu Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Thr Ile 
        115                 120                 125             


Lys Gly Lys Ser Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys 
    130                 135                 140                 


Asp Gly Asn Leu Ala Val Ile Gly Val Ile Phe Lys Glu Gly Lys Lys 
145                 150                 155                 160 


Asn Pro Ile Ile Glu Lys Ile Trp Glu Asn Leu Pro Glu Ala Gly Lys 
                165                 170                 175     


Thr Ile Lys Leu Ala His Lys Ile Asn Ala Tyr Asp Leu Leu Pro Lys 
            180                 185                 190         


Lys Lys Lys Tyr Tyr Arg Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Leu Ser 
    210                 215                 220                 


Lys Glu Gln Ile Glu Lys Phe Arg Lys Leu Met Gly Gly Asp Thr Asn 
225                 230                 235                 240 


Arg Pro Val Gln Pro Leu Asn Ala Arg Met Ile Met Glu Met Asp 
                245                 250                 255 


<210>  12
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4

<400>  12
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggagttggac attggtctta tcatggcgaa      120

acaggaccgc aacattgggg cgatctgaaa aacgaataca tcatgtgcaa aatcggcaaa      180

aaccagtcac cggtggatat tagcagaatc gtcgaagctg aacttgaaaa aatcaaaatc      240

aactattcaa gcggcggatc ttcaatcaca aacaacggac atacaatcaa agtttcttat      300

gaaccgggat catatattat cgtggatggc attcgctttg aattaaaaca atttcatttt      360

catgccccga gcgaacatac aatcaaaggc aaatcttatc cgtttgaagc acattttgtc      420

catgcggata aagatggcaa tctggcagtt attggagtga tctttaaaga aggcaagaaa      480

aatccgatca tcgaaaaaat ttgggaaaac ttaccggaag cgggcaaaac aatcaaactg      540

gctcataaaa tcaacgccta tgatctgctt ccgaaaaaga aaaaatacta cagatacagc      600

ggatctctta caacaccgcc gtgttcagaa ggcgtccgct ggattgttat ggaagaagaa      660

atggaactta gcaaagaaca aatcgaaaaa tttagaaaac tgatgggcgg agatacaaat      720

agaccggttc agccgttaaa cgctcgcatg attatggaaa tggattaa                   768


<210>  13
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut2

<400>  13

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Val 
            20                  25                  30          


Gly His Trp Ser Tyr His Gly Glu Thr Gly Pro Gln His Trp Gly Asp 
        35                  40                  45              


Leu Lys Asn Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro 
    50                  55                  60                  


Val Asp Ile Ser Arg Ile Val Glu Cys Glu Leu Glu Lys Ile Lys Ile 
65                  70                  75                  80  


Asn Tyr Ser Ser Gly Gly Ser Ser Ile Thr Asn Asn Gly His Thr Ile 
                85                  90                  95      


Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Ile Val Asp Gly Ile Arg 
            100                 105                 110         


Phe Glu Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Thr Ile 
        115                 120                 125             


Lys Gly Lys Ser Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys 
    130                 135                 140                 


Asp Gly Asn Leu Ala Val Ile Gly Val Ile Phe Lys Glu Gly Lys Lys 
145                 150                 155                 160 


Asn Pro Ile Ile Glu Lys Ile Trp Glu Asn Leu Pro Glu Ala Gly Lys 
                165                 170                 175     


Thr Ile Lys Leu Ala His Lys Ile Asn Ala Tyr Asp Leu Leu Pro Lys 
            180                 185                 190         


Lys Lys Lys Tyr Tyr Arg Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Leu Ser 
    210                 215                 220                 


Lys Glu Gln Ile Glu Lys Phe Arg Lys Leu Met Gly Gly Asp Thr Asn 
225                 230                 235                 240 


Arg Pro Val Gln Pro Leu Asn Ala Arg Met Ile Met Glu Met Asp 
                245                 250                 255 


<210>  14
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut2

<400>  14
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggagttggac attggtctta tcatggcgaa      120

acaggaccgc aacattgggg cgatctgaaa aacgaataca tcatgtgcaa aatcggcaaa      180

aaccagtcac cggtggatat tagcagaatc gtcgaatgtg aacttgaaaa aatcaaaatc      240

aactattcaa gcggcggatc ttcaatcaca aacaacggac atacaatcaa agtttcttat      300

gaaccgggat catatattat cgtggatggc attcgctttg aattaaaaca atttcatttt      360

catgccccga gcgaacatac aatcaaaggc aaatcttatc cgtttgaagc acattttgtc      420

catgcggata aagatggcaa tctggcagtt attggagtga tctttaaaga aggcaagaaa      480

aatccgatca tcgaaaaaat ttgggaaaac ttaccggaag cgggcaaaac aatcaaactg      540

gctcataaaa tcaacgccta tgatctgctt ccgaaaaaga aaaaatacta cagatacagc      600

ggatctctta caacaccgcc gtgttcagaa ggcgtccgct ggattgttat ggaagaagaa      660

atggaactta gcaaagaaca aatcgaaaaa tttagaaaac tgatgggcgg agatacaaat      720

agaccggttc agccgttaaa cgctcgcatg attatggaaa tggattaa                   768


<210>  15
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut3

<400>  15

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Val 
            20                  25                  30          


Gly His Trp Ser Tyr His Gly Glu Thr Gly Pro Gln His Trp Gly Asp 
        35                  40                  45              


Leu Lys Asn Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro 
    50                  55                  60                  


Val Asp Ile Ser Arg Ile Val Glu Ala Glu Leu Glu Lys Ile Lys Ile 
65                  70                  75                  80  


Asn Tyr Ser Ser Gly Gly Ser Ser Ile Thr Asn Asn Gly His Thr Ile 
                85                  90                  95      


Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Ile Val Asp Gly Ile Arg 
            100                 105                 110         


Phe Glu Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Thr Ile 
        115                 120                 125             


Lys Gly Lys Ser Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys 
    130                 135                 140                 


Asp Gly Asn Leu Ala Val Ile Gly Val Ile Phe Lys Glu Gly Lys Lys 
145                 150                 155                 160 


Asn Pro Ile Ile Glu Lys Ile Trp Glu Asn Leu Pro Glu Ala Gly Lys 
                165                 170                 175     


Thr Ile Lys Leu Ala His Lys Ile Asn Ala Tyr Asp Leu Leu Pro Lys 
            180                 185                 190         


Lys Lys Lys Tyr Tyr Arg Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Leu Ser 
    210                 215                 220                 


Lys Glu Gln Ile Glu Lys Phe Arg Lys Leu Met Gly Gly Asp Thr Asn 
225                 230                 235                 240 


Arg Pro Val Gln Pro Leu Asn Cys Arg Met Ile Met Glu Met Asp 
                245                 250                 255 


<210>  16
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut3

<400>  16
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggagttggac attggtctta tcatggcgaa      120

acaggaccgc aacattgggg cgatctgaaa aacgaataca tcatgtgcaa aatcggcaaa      180

aaccagtcac cggtggatat tagcagaatc gtcgaagctg aacttgaaaa aatcaaaatc      240

aactattcaa gcggcggatc ttcaatcaca aacaacggac atacaatcaa agtttcttat      300

gaaccgggat catatattat cgtggatggc attcgctttg aattaaaaca atttcatttt      360

catgccccga gcgaacatac aatcaaaggc aaatcttatc cgtttgaagc acattttgtc      420

catgcggata aagatggcaa tctggcagtt attggagtga tctttaaaga aggcaagaaa      480

aatccgatca tcgaaaaaat ttgggaaaac ttaccggaag cgggcaaaac aatcaaactg      540

gctcataaaa tcaacgccta tgatctgctt ccgaaaaaga aaaaatacta cagatactgc      600

ggatctctta caacaccgcc gtgttcagaa ggcgtccgct ggattgttat ggaagaagaa      660

atggaactta gcaaagaaca aatcgaaaaa tttagaaaac tgatgggcgg agatacaaat      720

agaccggttc agccgttaaa ctgtcgcatg attatggaaa tggattaa                   768


<210>  17
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut23

<400>  17

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Gly Val 
            20                  25                  30          


Gly His Trp Ser Tyr His Gly Glu Thr Gly Pro Gln His Trp Gly Asp 
        35                  40                  45              


Leu Lys Asn Glu Tyr Ile Met Cys Lys Ile Gly Lys Asn Gln Ser Pro 
    50                  55                  60                  


Val Asp Ile Ser Arg Ile Val Glu Cys Glu Leu Glu Lys Ile Lys Ile 
65                  70                  75                  80  


Asn Tyr Ser Ser Gly Gly Ser Ser Ile Thr Asn Asn Gly His Thr Ile 
                85                  90                  95      


Lys Val Ser Tyr Glu Pro Gly Ser Tyr Ile Ile Val Asp Gly Ile Arg 
            100                 105                 110         


Phe Glu Leu Lys Gln Phe His Phe His Ala Pro Ser Glu His Thr Ile 
        115                 120                 125             


Lys Gly Lys Ser Tyr Pro Phe Glu Ala His Phe Val His Ala Asp Lys 
    130                 135                 140                 


Asp Gly Asn Leu Ala Val Ile Gly Val Ile Phe Lys Glu Gly Lys Lys 
145                 150                 155                 160 


Asn Pro Ile Ile Glu Lys Ile Trp Glu Asn Leu Pro Glu Ala Gly Lys 
                165                 170                 175     


Thr Ile Lys Leu Ala His Lys Ile Asn Ala Tyr Asp Leu Leu Pro Lys 
            180                 185                 190         


Lys Lys Lys Tyr Tyr Arg Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Ser Glu Gly Val Arg Trp Ile Val Met Glu Glu Glu Met Glu Leu Ser 
    210                 215                 220                 


Lys Glu Gln Ile Glu Lys Phe Arg Lys Leu Met Gly Gly Asp Thr Asn 
225                 230                 235                 240 


Arg Pro Val Gln Pro Leu Asn Cys Arg Met Ile Met Glu Met Asp 
                245                 250                 255 


<210>  18
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-LOGACA-CA4mut23

<400>  18
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc ggagttggac attggtctta tcatggcgaa      120

acaggaccgc aacattgggg cgatctgaaa aacgaataca tcatgtgcaa aatcggcaaa      180

aaccagtcac cggtggatat tagcagaatc gtcgaatgtg aacttgaaaa aatcaaaatc      240

aactattcaa gcggcggatc ttcaatcaca aacaacggac atacaatcaa agtttcttat      300

gaaccgggat catatattat cgtggatggc attcgctttg aattaaaaca atttcatttt      360

catgccccga gcgaacatac aatcaaaggc aaatcttatc cgtttgaagc acattttgtc      420

catgcggata aagatggcaa tctggcagtt attggagtga tctttaaaga aggcaagaaa      480

aatccgatca tcgaaaaaat ttgggaaaac ttaccggaag cgggcaaaac aatcaaactg      540

gctcataaaa tcaacgccta tgatctgctt ccgaaaaaga aaaaatacta cagatactgc      600

ggatctctta caacaccgcc gtgttcagaa ggcgtccgct ggattgttat ggaagaagaa      660

atggaactta gcaaagaaca aatcgaaaaa tttagaaaac tgatgggcgg agatacaaat      720

agaccggttc agccgttaaa ctgtcgcatg attatggaaa tggattaa                   768


<210>  19
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5

<400>  19

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Glu His Glu 
            20                  25                  30          


Trp Ser Tyr Glu Gly Glu Lys Gly Pro Glu His Trp Ala Gln Leu Lys 
        35                  40                  45              


Pro Glu Phe Phe Trp Cys Lys Leu Lys Asn Gln Ser Pro Ile Asn Ile 
    50                  55                  60                  


Asp Lys Lys Tyr Lys Val Lys Ala Asn Leu Pro Lys Leu Asn Leu Tyr 
65                  70                  75                  80  


Tyr Lys Thr Ala Lys Glu Ser Glu Val Val Asn Asn Gly His Thr Ile 
                85                  90                  95      


Gln Ile Asn Ile Lys Glu Asp Asn Thr Leu Asn Tyr Leu Gly Glu Lys 
            100                 105                 110         


Tyr Gln Leu Lys Gln Phe His Phe His Thr Pro Ser Glu His Thr Ile 
        115                 120                 125             


Glu Lys Lys Ser Tyr Pro Leu Glu Ile His Phe Val His Lys Thr Glu 
    130                 135                 140                 


Asp Gly Lys Ile Leu Val Val Gly Val Met Ala Lys Leu Gly Lys Thr 
145                 150                 155                 160 


Asn Lys Glu Leu Asp Lys Ile Leu Asn Val Ala Pro Ala Glu Glu Gly 
                165                 170                 175     


Glu Lys Ile Leu Asp Lys Asn Leu Asn Leu Asn Asn Leu Ile Pro Lys 
            180                 185                 190         


Asp Lys Arg Tyr Met Thr Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Thr Glu Gly Val Arg Trp Ile Val Leu Lys Lys Pro Ile Ser Ile Ser 
    210                 215                 220                 


Lys Gln Gln Leu Glu Lys Leu Lys Ser Val Met Val Asn Pro Asn Asn 
225                 230                 235                 240 


Arg Pro Val Gln Glu Ile Asn Ser Arg Trp Ile Ile Glu Gly Phe 
                245                 250                 255 


<210>  20
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5

<400>  20
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccgaa catgaatggt cttatgaagg cgaaaaagga      120

ccggaacatt gggcacaact gaaaccggaa tttttctggt gcaaacttaa aaaccagtca      180

ccgatcaaca tcgataaaaa atacaaagtt aaagctaacc tgccgaaact gaacctttac      240

tacaaaacag ccaaagaatc agaagttgtg aataacggac atacaatcca aatcaacatc      300

aaagaagata acacacttaa ctacctgggc gaaaaatacc aactgaaaca gtttcatttt      360

catacaccga gcgaacatac aatcgagaaa aaatcatacc cgcttgaaat ccattttgtc      420

cataaaacag aagatggcaa aatccttgtc gttggagtta tggctaaact gggcaaaaca      480

aacaaagaat tagataaaat tctgaacgtg gcaccggcgg aagaaggaga aaaaatctta      540

gataaaaacc tgaacctgaa caacctgatc ccgaaagata aaagatacat gacatactca      600

ggaagcctta caacaccgcc gtgtacagaa ggcgttcgct ggatcgtgct gaaaaaaccg      660

atctctattt caaaacaaca gctggaaaaa cttaaatcag tgatggtcaa tccgaataac      720

agaccggtcc aggaaattaa cagccgctgg attatcgaag gcttttaa                   768


<210>  21
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut2

<400>  21

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Glu His Glu 
            20                  25                  30          


Trp Ser Tyr Glu Gly Glu Lys Gly Pro Glu His Trp Ala Gln Leu Lys 
        35                  40                  45              


Pro Glu Phe Phe Trp Cys Lys Leu Lys Asn Gln Ser Pro Ile Asn Ile 
    50                  55                  60                  


Asp Lys Lys Tyr Lys Val Lys Cys Asn Leu Pro Lys Leu Asn Leu Tyr 
65                  70                  75                  80  


Tyr Lys Thr Ala Lys Glu Ser Glu Val Val Asn Asn Gly His Thr Ile 
                85                  90                  95      


Gln Ile Asn Ile Lys Glu Asp Asn Thr Leu Asn Tyr Leu Gly Glu Lys 
            100                 105                 110         


Tyr Gln Leu Lys Gln Phe His Phe His Thr Pro Ser Glu His Thr Ile 
        115                 120                 125             


Glu Lys Lys Ser Tyr Pro Leu Glu Ile His Phe Val His Lys Thr Glu 
    130                 135                 140                 


Asp Gly Lys Ile Leu Val Val Gly Val Met Ala Lys Leu Gly Lys Thr 
145                 150                 155                 160 


Asn Lys Glu Leu Asp Lys Ile Leu Asn Val Ala Pro Ala Glu Glu Gly 
                165                 170                 175     


Glu Lys Ile Leu Asp Lys Asn Leu Asn Leu Asn Asn Leu Ile Pro Lys 
            180                 185                 190         


Asp Lys Arg Tyr Met Thr Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Thr Glu Gly Val Arg Trp Ile Val Leu Lys Lys Pro Ile Ser Ile Ser 
    210                 215                 220                 


Lys Gln Gln Leu Glu Lys Leu Lys Ser Val Met Val Asn Pro Asn Asn 
225                 230                 235                 240 


Arg Pro Val Gln Glu Ile Asn Ser Arg Trp Ile Ile Glu Gly Phe 
                245                 250                 255 


<210>  22
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut2

<400>  22
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccgaa catgaatggt cttatgaagg cgaaaaagga      120

ccggaacatt gggcacaact gaaaccggaa tttttctggt gcaaacttaa aaaccagtca      180

ccgatcaaca tcgataaaaa atacaaagtt aaatgtaacc tgccgaaact gaacctttac      240

tacaaaacag ccaaagaatc agaagttgtg aataacggac atacaatcca aatcaacatc      300

aaagaagata acacacttaa ctacctgggc gaaaaatacc aactgaaaca gtttcatttt      360

catacaccga gcgaacatac aatcgagaaa aaatcatacc cgcttgaaat ccattttgtc      420

cataaaacag aagatggcaa aatccttgtc gttggagtta tggctaaact gggcaaaaca      480

aacaaagaat tagataaaat tctgaacgtg gcaccggcgg aagaaggaga aaaaatctta      540

gataaaaacc tgaacctgaa caacctgatc ccgaaagata aaagatacat gacatactca      600

ggaagcctta caacaccgcc gtgtacagaa ggcgttcgct ggatcgtgct gaaaaaaccg      660

atctctattt caaaacaaca gctggaaaaa cttaaatcag tgatggtcaa tccgaataac      720

agaccggtcc aggaaattaa cagccgctgg attatcgaag gcttttaa                   768


<210>  23
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut3

<400>  23

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Glu His Glu 
            20                  25                  30          


Trp Ser Tyr Glu Gly Glu Lys Gly Pro Glu His Trp Ala Gln Leu Lys 
        35                  40                  45              


Pro Glu Phe Phe Trp Cys Lys Leu Lys Asn Gln Ser Pro Ile Asn Ile 
    50                  55                  60                  


Asp Lys Lys Tyr Lys Val Lys Ala Asn Leu Pro Lys Leu Asn Leu Tyr 
65                  70                  75                  80  


Tyr Lys Thr Ala Lys Glu Ser Glu Val Val Asn Asn Gly His Thr Ile 
                85                  90                  95      


Gln Ile Asn Ile Lys Glu Asp Asn Thr Leu Asn Tyr Leu Gly Glu Lys 
            100                 105                 110         


Tyr Gln Leu Lys Gln Phe His Phe His Thr Pro Ser Glu His Thr Ile 
        115                 120                 125             


Glu Lys Lys Ser Tyr Pro Leu Glu Ile His Phe Val His Lys Thr Glu 
    130                 135                 140                 


Asp Gly Lys Ile Leu Val Val Gly Val Met Ala Lys Leu Gly Lys Thr 
145                 150                 155                 160 


Asn Lys Glu Leu Asp Lys Ile Leu Asn Val Ala Pro Ala Glu Glu Gly 
                165                 170                 175     


Glu Lys Ile Leu Asp Lys Asn Leu Asn Leu Asn Asn Leu Ile Pro Lys 
            180                 185                 190         


Asp Lys Arg Tyr Met Thr Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Thr Glu Gly Val Arg Trp Ile Val Leu Lys Lys Pro Ile Ser Ile Ser 
    210                 215                 220                 


Lys Gln Gln Leu Glu Lys Leu Lys Ser Val Met Val Asn Pro Asn Asn 
225                 230                 235                 240 


Arg Pro Val Gln Glu Ile Asn Cys Arg Trp Ile Ile Glu Gly Phe 
                245                 250                 255 


<210>  24
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut3

<400>  24
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccgaa catgaatggt cttatgaagg cgaaaaagga      120

ccggaacatt gggcacaact gaaaccggaa tttttctggt gcaaacttaa aaaccagtca      180

ccgatcaaca tcgataaaaa atacaaagtt aaagctaacc tgccgaaact gaacctttac      240

tacaaaacag ccaaagaatc agaagttgtg aataacggac atacaatcca aatcaacatc      300

aaagaagata acacacttaa ctacctgggc gaaaaatacc aactgaaaca gtttcatttt      360

catacaccga gcgaacatac aatcgagaaa aaatcatacc cgcttgaaat ccattttgtc      420

cataaaacag aagatggcaa aatccttgtc gttggagtta tggctaaact gggcaaaaca      480

aacaaagaat tagataaaat tctgaacgtg gcaccggcgg aagaaggaga aaaaatctta      540

gataaaaacc tgaacctgaa caacctgatc ccgaaagata aaagatacat gacatactgc      600

ggaagcctta caacaccgcc gtgtacagaa ggcgttcgct ggatcgtgct gaaaaaaccg      660

atctctattt caaaacaaca gctggaaaaa cttaaatcag tgatggtcaa tccgaataac      720

agaccggtcc aggaaattaa ctgtcgctgg attatcgaag gcttttaa                   768


<210>  25
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut23

<400>  25

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Glu His Glu 
            20                  25                  30          


Trp Ser Tyr Glu Gly Glu Lys Gly Pro Glu His Trp Ala Gln Leu Lys 
        35                  40                  45              


Pro Glu Phe Phe Trp Cys Lys Leu Lys Asn Gln Ser Pro Ile Asn Ile 
    50                  55                  60                  


Asp Lys Lys Tyr Lys Val Lys Cys Asn Leu Pro Lys Leu Asn Leu Tyr 
65                  70                  75                  80  


Tyr Lys Thr Ala Lys Glu Ser Glu Val Val Asn Asn Gly His Thr Ile 
                85                  90                  95      


Gln Ile Asn Ile Lys Glu Asp Asn Thr Leu Asn Tyr Leu Gly Glu Lys 
            100                 105                 110         


Tyr Gln Leu Lys Gln Phe His Phe His Thr Pro Ser Glu His Thr Ile 
        115                 120                 125             


Glu Lys Lys Ser Tyr Pro Leu Glu Ile His Phe Val His Lys Thr Glu 
    130                 135                 140                 


Asp Gly Lys Ile Leu Val Val Gly Val Met Ala Lys Leu Gly Lys Thr 
145                 150                 155                 160 


Asn Lys Glu Leu Asp Lys Ile Leu Asn Val Ala Pro Ala Glu Glu Gly 
                165                 170                 175     


Glu Lys Ile Leu Asp Lys Asn Leu Asn Leu Asn Asn Leu Ile Pro Lys 
            180                 185                 190         


Asp Lys Arg Tyr Met Thr Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys 
        195                 200                 205             


Thr Glu Gly Val Arg Trp Ile Val Leu Lys Lys Pro Ile Ser Ile Ser 
    210                 215                 220                 


Lys Gln Gln Leu Glu Lys Leu Lys Ser Val Met Val Asn Pro Asn Asn 
225                 230                 235                 240 


Arg Pro Val Gln Glu Ile Asn Cys Arg Trp Ile Ile Glu Gly Phe 
                245                 250                 255 


<210>  26
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SspCA-CA5mut23

<400>  26
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccgaa catgaatggt cttatgaagg cgaaaaagga      120

ccggaacatt gggcacaact gaaaccggaa tttttctggt gcaaacttaa aaaccagtca      180

ccgatcaaca tcgataaaaa atacaaagtt aaatgtaacc tgccgaaact gaacctttac      240

tacaaaacag ccaaagaatc agaagttgtg aataacggac atacaatcca aatcaacatc      300

aaagaagata acacacttaa ctacctgggc gaaaaatacc aactgaaaca gtttcatttt      360

catacaccga gcgaacatac aatcgagaaa aaatcatacc cgcttgaaat ccattttgtc      420

cataaaacag aagatggcaa aatccttgtc gttggagtta tggctaaact gggcaaaaca      480

aacaaagaat tagataaaat tctgaacgtg gcaccggcgg aagaaggaga aaaaatctta      540

gataaaaacc tgaacctgaa caacctgatc ccgaaagata aaagatacat gacatactgc      600

ggaagcctta caacaccgcc gtgtacagaa ggcgttcgct ggatcgtgct gaaaaaaccg      660

atctctattt caaaacaaca gctggaaaaa cttaaatcag tgatggtcaa tccgaataac      720

agaccggtcc aggaaattaa ctgtcgctgg attatcgaag gcttttaa                   768


<210>  27
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FL

<400>  27

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Glu His 
            20                  25                  30          


Ala Ile Leu Gln Lys Asn Ala Glu Val His His Trp Ser Tyr Glu Gly 
        35                  40                  45              


Glu Asn Gly Pro Glu Asn Trp Ala Lys Leu Asn Pro Glu Tyr Phe Trp 
    50                  55                  60                  


Cys Asn Leu Lys Asn Gln Ser Pro Val Asp Ile Ser Asp Asn Tyr Lys 
65                  70                  75                  80  


Val His Ala Lys Leu Glu Lys Leu His Ile Asn Tyr Asn Lys Ala Val 
                85                  90                  95      


Asn Pro Glu Ile Val Asn Asn Gly His Thr Ile Gln Val Asn Val Leu 
            100                 105                 110         


Glu Asp Phe Lys Leu Asn Ile Lys Gly Lys Glu Tyr His Leu Lys Gln 
        115                 120                 125             


Phe His Phe His Ala Pro Ser Glu His Thr Val Asn Gly Lys Tyr Tyr 
    130                 135                 140                 


Pro Leu Glu Met His Leu Val His Lys Asp Lys Asp Gly Asn Ile Ala 
145                 150                 155                 160 


Val Ile Gly Val Phe Phe Lys Glu Gly Lys Ala Asn Pro Glu Leu Asp 
                165                 170                 175     


Lys Val Phe Lys Asn Ala Leu Lys Glu Glu Gly Ser Lys Val Phe Asp 
            180                 185                 190         


Gly Ser Ile Asn Ile Asn Ala Leu Leu Pro Pro Val Lys Asn Tyr Tyr 
        195                 200                 205             


Thr Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys Thr Glu Gly Val Leu 
    210                 215                 220                 


Trp Ile Val Leu Lys Gln Pro Ile Thr Ala Ser Lys Gln Gln Ile Glu 
225                 230                 235                 240 


Leu Phe Lys Ser Ile Met Lys His Asn Asn Asn Arg Pro Thr Gln Pro 
                245                 250                 255     


Ile Asn Ser Arg Tyr Ile Leu Glu Ser Asn 
            260                 265     


<210>  28
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FL

<400>  28
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc gaacatgcaa ttctgcagaa aaatgcggaa      120

gtccatcatt ggagctatga aggcgaaaac ggaccggaaa attgggccaa actgaacccg      180

gaatactttt ggtgcaacct taaaaaccag tctccggtcg atatttcaga taactacaaa      240

gttcatgcca aactggaaaa actgcatatc aactacaaca aagcagttaa cccggaaatt      300

gtgaataacg gacatacaat ccaagttaac gtgttagaag attttaaact gaacatcaaa      360

ggcaaagaat accatcttaa acagtttcat tttcatgctc cgtctgaaca tacagtgaac      420

ggcaaatatt atccgcttga aatgcatctg gtccataaag ataaagatgg caacattgca      480

gtcatcggag ttttctttaa agaaggcaaa gcgaacccgg aacttgataa agtttttaaa      540

aacgctctga aagaagaagg aagcaaagtg tttgatggct ctattaacat caatgcgctg      600

cttccgccgg ttaaaaacta ctacacatac tcaggaagct taacaacacc gccgtgtaca      660

gaaggcgtgc tgtggattgt ccttaaacaa ccgatcacag cttctaaaca acagattgaa      720

ctgtttaaat caatcatgaa acataacaac aatagaccga cacagccgat taactcacgc      780

tatatcctgg aaagcaatta a                                                801


<210>  29
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut2

<400>  29

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Glu His 
            20                  25                  30          


Ala Ile Leu Gln Lys Asn Ala Glu Val His His Trp Ser Tyr Glu Gly 
        35                  40                  45              


Glu Asn Gly Pro Glu Asn Trp Ala Lys Leu Asn Pro Glu Tyr Phe Trp 
    50                  55                  60                  


Cys Asn Leu Lys Asn Gln Ser Pro Val Asp Ile Ser Asp Asn Tyr Lys 
65                  70                  75                  80  


Val His Cys Lys Leu Glu Lys Leu His Ile Asn Tyr Asn Lys Ala Val 
                85                  90                  95      


Asn Pro Glu Ile Val Asn Asn Gly His Thr Ile Gln Val Asn Val Leu 
            100                 105                 110         


Glu Asp Phe Lys Leu Asn Ile Lys Gly Lys Glu Tyr His Leu Lys Gln 
        115                 120                 125             


Phe His Phe His Ala Pro Ser Glu His Thr Val Asn Gly Lys Tyr Tyr 
    130                 135                 140                 


Pro Leu Glu Met His Leu Val His Lys Asp Lys Asp Gly Asn Ile Ala 
145                 150                 155                 160 


Val Ile Gly Val Phe Phe Lys Glu Gly Lys Ala Asn Pro Glu Leu Asp 
                165                 170                 175     


Lys Val Phe Lys Asn Ala Leu Lys Glu Glu Gly Ser Lys Val Phe Asp 
            180                 185                 190         


Gly Ser Ile Asn Ile Asn Ala Leu Leu Pro Pro Val Lys Asn Tyr Tyr 
        195                 200                 205             


Thr Tyr Ser Gly Ser Leu Thr Thr Pro Pro Cys Thr Glu Gly Val Leu 
    210                 215                 220                 


Trp Ile Val Leu Lys Gln Pro Ile Thr Ala Ser Lys Gln Gln Ile Glu 
225                 230                 235                 240 


Leu Phe Lys Ser Ile Met Lys His Asn Asn Asn Arg Pro Thr Gln Pro 
                245                 250                 255     


Ile Asn Ser Arg Tyr Ile Leu Glu Ser Asn 
            260                 265     


<210>  30
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut2

<400>  30
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc gaacatgcaa ttctgcagaa aaatgcggaa      120

gtccatcatt ggagctatga aggcgaaaac ggaccggaaa attgggccaa actgaacccg      180

gaatactttt ggtgcaacct taaaaaccag tctccggtcg atatttcaga taactacaaa      240

gttcattgta aactggaaaa actgcatatc aactacaaca aagcagttaa cccggaaatt      300

gtgaataacg gacatacaat ccaagttaac gtgttagaag attttaaact gaacatcaaa      360

ggcaaagaat accatcttaa acagtttcat tttcatgctc cgtctgaaca tacagtgaac      420

ggcaaatatt atccgcttga aatgcatctg gtccataaag ataaagatgg caacattgca      480

gtcatcggag ttttctttaa agaaggcaaa gcgaacccgg aacttgataa agtttttaaa      540

aacgctctga aagaagaagg aagcaaagtg tttgatggct ctattaacat caatgcgctg      600

cttccgccgg ttaaaaacta ctacacatac tcaggaagct taacaacacc gccgtgtaca      660

gaaggcgtgc tgtggattgt ccttaaacaa ccgatcacag cttctaaaca acagattgaa      720

ctgtttaaat caatcatgaa acataacaac aatagaccga cacagccgat taactcacgc      780

tatatcctgg aaagcaatta a                                                801


<210>  31
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut3

<400>  31

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Glu His 
            20                  25                  30          


Ala Ile Leu Gln Lys Asn Ala Glu Val His His Trp Ser Tyr Glu Gly 
        35                  40                  45              


Glu Asn Gly Pro Glu Asn Trp Ala Lys Leu Asn Pro Glu Tyr Phe Trp 
    50                  55                  60                  


Cys Asn Leu Lys Asn Gln Ser Pro Val Asp Ile Ser Asp Asn Tyr Lys 
65                  70                  75                  80  


Val His Ala Lys Leu Glu Lys Leu His Ile Asn Tyr Asn Lys Ala Val 
                85                  90                  95      


Asn Pro Glu Ile Val Asn Asn Gly His Thr Ile Gln Val Asn Val Leu 
            100                 105                 110         


Glu Asp Phe Lys Leu Asn Ile Lys Gly Lys Glu Tyr His Leu Lys Gln 
        115                 120                 125             


Phe His Phe His Ala Pro Ser Glu His Thr Val Asn Gly Lys Tyr Tyr 
    130                 135                 140                 


Pro Leu Glu Met His Leu Val His Lys Asp Lys Asp Gly Asn Ile Ala 
145                 150                 155                 160 


Val Ile Gly Val Phe Phe Lys Glu Gly Lys Ala Asn Pro Glu Leu Asp 
                165                 170                 175     


Lys Val Phe Lys Asn Ala Leu Lys Glu Glu Gly Ser Lys Val Phe Asp 
            180                 185                 190         


Gly Ser Ile Asn Ile Asn Ala Leu Leu Pro Pro Val Lys Asn Tyr Tyr 
        195                 200                 205             


Thr Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys Thr Glu Gly Val Leu 
    210                 215                 220                 


Trp Ile Val Leu Lys Gln Pro Ile Thr Ala Ser Lys Gln Gln Ile Glu 
225                 230                 235                 240 


Leu Phe Lys Ser Ile Met Lys His Asn Asn Asn Arg Pro Thr Gln Pro 
                245                 250                 255     


Ile Asn Cys Arg Tyr Ile Leu Glu Ser Asn 
            260                 265     


<210>  32
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut3

<400>  32
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc gaacatgcaa ttctgcagaa aaatgcggaa      120

gtccatcatt ggagctatga aggcgaaaac ggaccggaaa attgggccaa actgaacccg      180

gaatactttt ggtgcaacct taaaaaccag tctccggtcg atatttcaga taactacaaa      240

gttcatgcca aactggaaaa actgcatatc aactacaaca aagcagttaa cccggaaatt      300

gtgaataacg gacatacaat ccaagttaac gtgttagaag attttaaact gaacatcaaa      360

ggcaaagaat accatcttaa acagtttcat tttcatgctc cgtctgaaca tacagtgaac      420

ggcaaatatt atccgcttga aatgcatctg gtccataaag ataaagatgg caacattgca      480

gtcatcggag ttttctttaa agaaggcaaa gcgaacccgg aacttgataa agtttttaaa      540

aacgctctga aagaagaagg aagcaaagtg tttgatggct ctattaacat caatgcgctg      600

cttccgccgg ttaaaaacta ctacacatac tgcggaagct taacaacacc gccgtgtaca      660

gaaggcgtgc tgtggattgt ccttaaacaa ccgatcacag cttctaaaca acagattgaa      720

ctgtttaaat caatcatgaa acataacaac aatagaccga cacagccgat taactgtcgc      780

tatatcctgg aaagcaatta a                                                801


<210>  33
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut23

<400>  33

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Gly Glu His 
            20                  25                  30          


Ala Ile Leu Gln Lys Asn Ala Glu Val His His Trp Ser Tyr Glu Gly 
        35                  40                  45              


Glu Asn Gly Pro Glu Asn Trp Ala Lys Leu Asn Pro Glu Tyr Phe Trp 
    50                  55                  60                  


Cys Asn Leu Lys Asn Gln Ser Pro Val Asp Ile Ser Asp Asn Tyr Lys 
65                  70                  75                  80  


Val His Cys Lys Leu Glu Lys Leu His Ile Asn Tyr Asn Lys Ala Val 
                85                  90                  95      


Asn Pro Glu Ile Val Asn Asn Gly His Thr Ile Gln Val Asn Val Leu 
            100                 105                 110         


Glu Asp Phe Lys Leu Asn Ile Lys Gly Lys Glu Tyr His Leu Lys Gln 
        115                 120                 125             


Phe His Phe His Ala Pro Ser Glu His Thr Val Asn Gly Lys Tyr Tyr 
    130                 135                 140                 


Pro Leu Glu Met His Leu Val His Lys Asp Lys Asp Gly Asn Ile Ala 
145                 150                 155                 160 


Val Ile Gly Val Phe Phe Lys Glu Gly Lys Ala Asn Pro Glu Leu Asp 
                165                 170                 175     


Lys Val Phe Lys Asn Ala Leu Lys Glu Glu Gly Ser Lys Val Phe Asp 
            180                 185                 190         


Gly Ser Ile Asn Ile Asn Ala Leu Leu Pro Pro Val Lys Asn Tyr Tyr 
        195                 200                 205             


Thr Tyr Cys Gly Ser Leu Thr Thr Pro Pro Cys Thr Glu Gly Val Leu 
    210                 215                 220                 


Trp Ile Val Leu Lys Gln Pro Ile Thr Ala Ser Lys Gln Gln Ile Glu 
225                 230                 235                 240 


Leu Phe Lys Ser Ile Met Lys His Asn Asn Asn Arg Pro Thr Gln Pro 
                245                 250                 255     


Ile Asn Cys Arg Tyr Ile Leu Glu Ser Asn 
            260                 265     


<210>  34
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPamyL-SazCA-CA6FLmut23

<400>  34
atgaaacaac agaaaagact gtatgcacgc ctgcttacat tactgtttgc tcttattttt       60

cttttaccgc attcagcagc ggctgccggc gaacatgcaa ttctgcagaa aaatgcggaa      120

gtccatcatt ggagctatga aggcgaaaac ggaccggaaa attgggccaa actgaacccg      180

gaatactttt ggtgcaacct taaaaaccag tctccggtcg atatttcaga taactacaaa      240

gttcattgta aactggaaaa actgcatatc aactacaaca aagcagttaa cccggaaatt      300

gtgaataacg gacatacaat ccaagttaac gtgttagaag attttaaact gaacatcaaa      360

ggcaaagaat accatcttaa acagtttcat tttcatgctc cgtctgaaca tacagtgaac      420

ggcaaatatt atccgcttga aatgcatctg gtccataaag ataaagatgg caacattgca      480

gtcatcggag ttttctttaa agaaggcaaa gcgaacccgg aacttgataa agtttttaaa      540

aacgctctga aagaagaagg aagcaaagtg tttgatggct ctattaacat caatgcgctg      600

cttccgccgg ttaaaaacta ctacacatac tgcggaagct taacaacacc gccgtgtaca      660

gaaggcgtgc tgtggattgt ccttaaacaa ccgatcacag cttctaaaca acagattgaa      720

ctgtttaaat caatcatgaa acataacaac aatagaccga cacagccgat taactgtcgc      780

tatatcctgg aaagcaatta a                                                801


<210>  35
<211>  29
<212>  PRT
<213>  Bacillus licheniformis

<400>  35

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala 
            20                  25                  


