                               SEQUENCE LISTING

<110> ENGENE, INC.
 
<120> COMBINATION GENE THERAPY FOR TREATMENT OF METASTATIC CANCER

<130> 189677/PCT

<140> PCT/US2022/017099
<141> 2022-02-18

<150> 63/150,846
<151> 2021-02-18

<160> 10    

<170> PatentIn version 3.5

<210> 1
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 1
Gly Ser Gly Ser Ser Arg Gly Gly Ser Gly Ser Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Gly Ser Lys 
            20  


<210> 2
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(6)
<223> A or S

<400> 2
Gly Ser Thr Ser Gly Xaa Gly Lys Ser Ser Glu Gly Lys Gly 
1               5                   10                  


<210> 3
<211> 18
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Gly Gly Gly Gly Gly Gly Ser 
1               5           


<210> 5
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210> 6
<211> 1611
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 6
atgtgccatc agcaacttgt catctcctgg ttctccctcg tgttcctggc ctcccctctt       60

gtcgcgattt gggagctgaa gaaagatgtg tacgtcgtgg aactcgactg gtacccggac      120

gcccccgggg aaatggtggt gctcacttgt gatactcccg aagaggatgg aattacctgg      180

accctcgatc agtcctccga ggtcttggga tccggcaaaa ctctgaccat ccaagtcaag      240

gaattcggcg acgcggggca gtacacctgt cacaagggcg gagaagtgct gtcgcactca      300

ctcctgctcc ttcacaaaaa ggaggacggc atctggtcga ccgacatcct gaaggaccag      360

aaggaaccca agaacaagac ctttctgcgc tgcgaggcca agaactattc gggaaggttc      420

acctgttggt ggctgactac catctccacc gacctgactt tctccgtgaa gtcctctcgg      480

ggttcgagcg acccgcaggg tgttacgtgc ggtgctgcaa ccctgtccgc ggagagagtg      540

cggggggaca acaaggaata cgagtactca gtggaatgcc aggaagatag cgcctgccct      600

gccgccgaag agtccctgcc gattgaagtc atggtggacg cagtgcataa gttgaaatat      660

gagaactaca cctcgtcgtt cttcatccgg gacatcatca agcctgaccc ccctaagaat      720

ctgcagctca agcccctcaa gaactccaga caggtcgaag tgtcctggga gtacccagat      780

acgtggagca caccgcactc gtacttctcc ttgaccttct gcgtccaagt gcagggaaag      840

tccaaacggg agaagaagga ccgcgtgttc actgataaga cttccgctac tgtgatctgc      900

cgcaaaaacg ccagcatcag cgtgcgcgcg caagatagat actactcaag ctcttggtcc      960

gaatgggcgt ccgtgccatg ctcggtgccc ggcgtgggcg tgcctggagt gggagcccgg     1020

aacttgccgg tggccacccc tgaccccgga atgttccctt gcctgcacca ctcccaaaac     1080

cttctgaggg ctgtgtccaa catgctgcag aaggctcggc agaccctgga attctacccc     1140

tgcacctccg aggagatcga ccacgaagat attaccaagg acaagacctc aaccgtggaa     1200

gcctgcctgc ccctggaact gaccaagaac gaatcgtgcc tgaatagccg ggaaacctcc     1260

ttcatcacca acggctcctg cctggcctca cgaaagacca gctttatgat ggccctgtgc     1320

ctgagctcga tctacgagga cctgaagatg taccaggtcg agttcaagac tatgaacgcc     1380

aagctgctga tggatccgaa gcggcagatc ttcttggacc agaatatgct ggcagtgatc     1440

gacgagctga tgcaggccct caacttcaac tccgagactg tgccgcaaaa gtcgagcctg     1500

gaggaaccgg acttctacaa gaccaagatc aagttatgta ttctcctgca cgcgtttagg     1560

attcgcgccg tgaccattga tagagtgatg tcctacctga acgccagctg a              1611


<210> 7
<211> 536
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser Val Pro Gly Val Gly Val Pro Gly 
                325                 330                 335     


Val Gly Ala Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met Phe 
            340                 345                 350         


Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn Met 
        355                 360                 365             


Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser Glu 
    370                 375                 380                 


Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val Glu 
385                 390                 395                 400 


Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn Ser 
                405                 410                 415     


Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg Lys 
            420                 425                 430         


Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp Leu 
        435                 440                 445             


Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu Met 
    450                 455                 460                 


Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val Ile 
465                 470                 475                 480 


Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro Gln 
                485                 490                 495     


Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys Leu 
            500                 505                 510         


Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp Arg 
        515                 520                 525             


Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535     


<210> 8
<211> 4156
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 8
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtggtaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat      360

cttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc      420

taccttaact taatgatttt gataaaaatc attaggtacc ccggctctag ttattaatag      480

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt      540

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg      600

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat      660

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct      720

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg      780

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg      840

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc      900

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa      960

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     1020

tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt     1080

tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggctcgca tctctccttc     1140

acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc     1200

tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc     1260

gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac     1320

gctttgcctg accctgcttg ctcaactcta gttctctcgt taacttaatg agacagatag     1380

aaactggtct tgtagaaaca gagtagtcgc ctgcttttct gccaggtgct gacttctctc     1440

ccctgggctt ttttcttttt ctcaggttga aaagaagaag acgaagaaga cgaagaagac     1500

aaaccgtcgt cgacgccgcc accatgtgcc atcagcaact tgtcatctcc tggttctccc     1560

tcgtgttcct ggcctcccct cttgtcgcga tttgggagct gaagaaagat gtgtacgtcg     1620

tggaactcga ctggtacccg gacgcccccg gggaaatggt ggtgctcact tgtgatactc     1680

ccgaagagga tggaattacc tggaccctcg atcagtcctc cgaggtcttg ggatccggca     1740

aaactctgac catccaagtc aaggaattcg gcgacgcggg gcagtacacc tgtcacaagg     1800

gcggagaagt gctgtcgcac tcactcctgc tccttcacaa aaaggaggac ggcatctggt     1860

cgaccgacat cctgaaggac cagaaggaac ccaagaacaa gacctttctg cgctgcgagg     1920

ccaagaacta ttcgggaagg ttcacctgtt ggtggctgac taccatctcc accgacctga     1980

ctttctccgt gaagtcctct cggggttcga gcgacccgca gggtgttacg tgcggtgctg     2040

caaccctgtc cgcggagaga gtgcgggggg acaacaagga atacgagtac tcagtggaat     2100

gccaggaaga tagcgcctgc cctgccgccg aagagtccct gccgattgaa gtcatggtgg     2160

acgcagtgca taagttgaaa tatgagaact acacctcgtc gttcttcatc cgggacatca     2220

tcaagcctga cccccctaag aatctgcagc tcaagcccct caagaactcc agacaggtcg     2280

aagtgtcctg ggagtaccca gatacgtgga gcacaccgca ctcgtacttc tccttgacct     2340

tctgcgtcca agtgcaggga aagtccaaac gggagaagaa ggaccgcgtg ttcactgata     2400

agacttccgc tactgtgatc tgccgcaaaa acgccagcat cagcgtgcgc gcgcaagata     2460

gatactactc aagctcttgg tccgaatggg cgtccgtgcc atgctcggtg cccggcgtgg     2520

gcgtgcctgg agtgggagcc cggaacttgc cggtggccac ccctgacccc ggaatgttcc     2580

cttgcctgca ccactcccaa aaccttctga gggctgtgtc caacatgctg cagaaggctc     2640

ggcagaccct ggaattctac ccctgcacct ccgaggagat cgaccacgaa gatattacca     2700

aggacaagac ctcaaccgtg gaagcctgcc tgcccctgga actgaccaag aacgaatcgt     2760

gcctgaatag ccgggaaacc tccttcatca ccaacggctc ctgcctggcc tcacgaaaga     2820

ccagctttat gatggccctg tgcctgagct cgatctacga ggacctgaag atgtaccagg     2880

tcgagttcaa gactatgaac gccaagctgc tgatggatcc gaagcggcag atcttcttgg     2940

accagaatat gctggcagtg atcgacgagc tgatgcaggc cctcaacttc aactccgaga     3000

ctgtgccgca aaagtcgagc ctggaggaac cggacttcta caagaccaag atcaagttat     3060

gtattctcct gcacgcgttt aggattcgcg ccgtgaccat tgatagagtg atgtcctacc     3120

tgaacgccag ctgagaattc ctgtgccttc tagttgccag ccatctgttg tttgcccctc     3180

ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga     3240

ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca     3300

ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc     3360

tatggcccgg gacggccgct agcaccgttg gtttccgtag tgtagtggtt atcacgttcg     3420

cctaacacgc gaaaggtccc cggttcgaaa ccgggcacta caaaccaaca acgttaaaaa     3480

acaggtcctc cccatactct ttcattgtac acaccgcaag ctcgacaatc atcggattga     3540

agcattgtcg cacacatctt ccacacagga tcagtacctg ctttcgcttt taaccaaggc     3600

ttttctccaa gggatattta tagtctcaaa acacacaatt actttacagt tagggtgagt     3660

ttccttttgt gctgtttttt aaaataataa tttagtattt gtatctctta tagaaatcca     3720

agcctatcat gtaaaatgta gctagtatta aaaagaacag attatctgtc ttttatcgca     3780

cattaagcct ctatagttac taggaaatat tatatgcaaa ttaaccgggg caggggagta     3840

gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc gggcctagag atggcggcgt     3900

cggatcggcc agcccgccta atgagcgggc ttttttttct tagggtgcaa aaggagagcc     3960

tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg     4020

accggggttc gagccccgta tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt     4080

gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc tccttttggc ttccttcccc     4140

taccggggcc gctagc                                                     4156


<210> 9
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Val Pro Gly Val Gly Val Pro Gly Val Gly 
1               5                   10  


<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220> 
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 10
Gly Gly Gly Gly Ser 
1               5   


