                         SEQUENCE LISTING

<110>  UNIVERSITY OF VIRGINIA PATENT FOUNDATION
       Caliari, Steven R.
       Hui, Erica
       Barker, Thomas H.
 
<120>  THE COMBINED INFLUENCE OF VISCOELASTIC AND ADHESIVE CUES ON 
       FIBROBLAST SPREADING AND FOCAL ADHESION FORMATION

<130>  3062/151 PCT

<150>  US 63/150,315
<151>  2021-02-17

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized peptide

<400>  1

Lys Lys Lys Cys Gly 
1               5   


<210>  2
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  2

Pro His Ser Arg Asn 
1               5   


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized peptide

<400>  3

Gly Cys Gly Tyr Gly Arg Gly Asp Ser Pro Gly 
1               5                   10      


<210>  4
<211>  2386
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Leu Arg Gly Pro Gly Pro Gly Leu Leu Leu Leu Ala Val Gln Cys 
1               5                   10                  15      


Leu Gly Thr Ala Val Pro Ser Thr Gly Ala Ser Lys Ser Lys Arg Gln 
            20                  25                  30          


Ala Gln Gln Met Val Gln Pro Gln Ser Pro Val Ala Val Ser Gln Ser 
        35                  40                  45              


Lys Pro Gly Cys Tyr Asp Asn Gly Lys His Tyr Gln Ile Asn Gln Gln 
    50                  55                  60                  


Trp Glu Arg Thr Tyr Leu Gly Asn Ala Leu Val Cys Thr Cys Tyr Gly 
65                  70                  75                  80  


Gly Ser Arg Gly Phe Asn Cys Glu Ser Lys Pro Glu Ala Glu Glu Thr 
                85                  90                  95      


Cys Phe Asp Lys Tyr Thr Gly Asn Thr Tyr Arg Val Gly Asp Thr Tyr 
            100                 105                 110         


Glu Arg Pro Lys Asp Ser Met Ile Trp Asp Cys Thr Cys Ile Gly Ala 
        115                 120                 125             


Gly Arg Gly Arg Ile Ser Cys Thr Ile Ala Asn Arg Cys His Glu Gly 
    130                 135                 140                 


Gly Gln Ser Tyr Lys Ile Gly Asp Thr Trp Arg Arg Pro His Glu Thr 
145                 150                 155                 160 


Gly Gly Tyr Met Leu Glu Cys Val Cys Leu Gly Asn Gly Lys Gly Glu 
                165                 170                 175     


Trp Thr Cys Lys Pro Ile Ala Glu Lys Cys Phe Asp His Ala Ala Gly 
            180                 185                 190         


Thr Ser Tyr Val Val Gly Glu Thr Trp Glu Lys Pro Tyr Gln Gly Trp 
        195                 200                 205             


Met Met Val Asp Cys Thr Cys Leu Gly Glu Gly Ser Gly Arg Ile Thr 
    210                 215                 220                 


Cys Thr Ser Arg Asn Arg Cys Asn Asp Gln Asp Thr Arg Thr Ser Tyr 
225                 230                 235                 240 


Arg Ile Gly Asp Thr Trp Ser Lys Lys Asp Asn Arg Gly Asn Leu Leu 
                245                 250                 255     


Gln Cys Ile Cys Thr Gly Asn Gly Arg Gly Glu Trp Lys Cys Glu Arg 
            260                 265                 270         


His Thr Ser Val Gln Thr Thr Ser Ser Gly Ser Gly Pro Phe Thr Asp 
        275                 280                 285             


Val Arg Ala Ala Val Tyr Gln Pro Gln Pro His Pro Gln Pro Pro Pro 
    290                 295                 300                 


Tyr Gly His Cys Val Thr Asp Ser Gly Val Val Tyr Ser Val Gly Met 
305                 310                 315                 320 


Gln Trp Leu Lys Thr Gln Gly Asn Lys Gln Met Leu Cys Thr Cys Leu 
                325                 330                 335     


Gly Asn Gly Val Ser Cys Gln Glu Thr Ala Val Thr Gln Thr Tyr Gly 
            340                 345                 350         


Gly Asn Ser Asn Gly Glu Pro Cys Val Leu Pro Phe Thr Tyr Asn Gly 
        355                 360                 365             


Arg Thr Phe Tyr Ser Cys Thr Thr Glu Gly Arg Gln Asp Gly His Leu 
    370                 375                 380                 


Trp Cys Ser Thr Thr Ser Asn Tyr Glu Gln Asp Gln Lys Tyr Ser Phe 
385                 390                 395                 400 


Cys Thr Asp His Thr Val Leu Val Gln Thr Arg Gly Gly Asn Ser Asn 
                405                 410                 415     


Gly Ala Leu Cys His Phe Pro Phe Leu Tyr Asn Asn His Asn Tyr Thr 
            420                 425                 430         


Asp Cys Thr Ser Glu Gly Arg Arg Asp Asn Met Lys Trp Cys Gly Thr 
        435                 440                 445             


Thr Gln Asn Tyr Asp Ala Asp Gln Lys Phe Gly Phe Cys Pro Met Ala 
    450                 455                 460                 


Ala His Glu Glu Ile Cys Thr Thr Asn Glu Gly Val Met Tyr Arg Ile 
465                 470                 475                 480 


Gly Asp Gln Trp Asp Lys Gln His Asp Met Gly His Met Met Arg Cys 
                485                 490                 495     


Thr Cys Val Gly Asn Gly Arg Gly Glu Trp Thr Cys Ile Ala Tyr Ser 
            500                 505                 510         


Gln Leu Arg Asp Gln Cys Ile Val Asp Asp Ile Thr Tyr Asn Val Asn 
        515                 520                 525             


Asp Thr Phe His Lys Arg His Glu Glu Gly His Met Leu Asn Cys Thr 
    530                 535                 540                 


Cys Phe Gly Gln Gly Arg Gly Arg Trp Lys Cys Asp Pro Val Asp Gln 
545                 550                 555                 560 


Cys Gln Asp Ser Glu Thr Gly Thr Phe Tyr Gln Ile Gly Asp Ser Trp 
                565                 570                 575     


Glu Lys Tyr Val His Gly Val Arg Tyr Gln Cys Tyr Cys Tyr Gly Arg 
            580                 585                 590         


Gly Ile Gly Glu Trp His Cys Gln Pro Leu Gln Thr Tyr Pro Ser Ser 
        595                 600                 605             


Ser Gly Pro Val Glu Val Phe Ile Thr Glu Thr Pro Ser Gln Pro Asn 
    610                 615                 620                 


Ser His Pro Ile Gln Trp Asn Ala Pro Gln Pro Ser His Ile Ser Lys 
625                 630                 635                 640 


Tyr Ile Leu Arg Trp Arg Pro Lys Asn Ser Val Gly Arg Trp Lys Glu 
                645                 650                 655     


Ala Thr Ile Pro Gly His Leu Asn Ser Tyr Thr Ile Lys Gly Leu Lys 
            660                 665                 670         


Pro Gly Val Val Tyr Glu Gly Gln Leu Ile Ser Ile Gln Gln Tyr Gly 
        675                 680                 685             


His Gln Glu Val Thr Arg Phe Asp Phe Thr Thr Thr Ser Thr Ser Thr 
    690                 695                 700                 


Pro Val Thr Ser Asn Thr Val Thr Gly Glu Thr Thr Pro Phe Ser Pro 
705                 710                 715                 720 


Leu Val Ala Thr Ser Glu Ser Val Thr Glu Ile Thr Ala Ser Ser Phe 
                725                 730                 735     


Val Val Ser Trp Val Ser Ala Ser Asp Thr Val Ser Gly Phe Arg Val 
            740                 745                 750         


Glu Tyr Glu Leu Ser Glu Glu Gly Asp Glu Pro Gln Tyr Leu Asp Leu 
        755                 760                 765             


Pro Ser Thr Ala Thr Ser Val Asn Ile Pro Asp Leu Leu Pro Gly Arg 
    770                 775                 780                 


Lys Tyr Ile Val Asn Val Tyr Gln Ile Ser Glu Asp Gly Glu Gln Ser 
785                 790                 795                 800 


Leu Ile Leu Ser Thr Ser Gln Thr Thr Ala Pro Asp Ala Pro Pro Asp 
                805                 810                 815     


Thr Thr Val Asp Gln Val Asp Asp Thr Ser Ile Val Val Arg Trp Ser 
            820                 825                 830         


Arg Pro Gln Ala Pro Ile Thr Gly Tyr Arg Ile Val Tyr Ser Pro Ser 
        835                 840                 845             


Val Glu Gly Ser Ser Thr Glu Leu Asn Leu Pro Glu Thr Ala Asn Ser 
    850                 855                 860                 


Val Thr Leu Ser Asp Leu Gln Pro Gly Val Gln Tyr Asn Ile Thr Ile 
865                 870                 875                 880 


Tyr Ala Val Glu Glu Asn Gln Glu Ser Thr Pro Val Val Ile Gln Gln 
                885                 890                 895     


Glu Thr Thr Gly Thr Pro Arg Ser Asp Thr Val Pro Ser Pro Arg Asp 
            900                 905                 910         


Leu Gln Phe Val Glu Val Thr Asp Val Lys Val Thr Ile Met Trp Thr 
        915                 920                 925             


Pro Pro Glu Ser Ala Val Thr Gly Tyr Arg Val Asp Val Ile Pro Val 
    930                 935                 940                 


Asn Leu Pro Gly Glu His Gly Gln Arg Leu Pro Ile Ser Arg Asn Thr 
945                 950                 955                 960 


Phe Ala Glu Val Thr Gly Leu Ser Pro Gly Val Thr Tyr Tyr Phe Lys 
                965                 970                 975     


Val Phe Ala Val Ser His Gly Arg Glu Ser Lys Pro Leu Thr Ala Gln 
            980                 985                 990         


Gln Thr Thr Lys Leu Asp Ala Pro  Thr Asn Leu Gln Phe  Val Asn Glu 
        995                 1000                 1005             


Thr Asp  Ser Thr Val Leu Val  Arg Trp Thr Pro Pro  Arg Ala Gln 
    1010                 1015                 1020             


Ile Thr  Gly Tyr Arg Leu Thr  Val Gly Leu Thr Arg  Arg Gly Gln 
    1025                 1030                 1035             


Pro Arg  Gln Tyr Asn Val Gly  Pro Ser Val Ser Lys  Tyr Pro Leu 
    1040                 1045                 1050             


Arg Asn  Leu Gln Pro Ala Ser  Glu Tyr Thr Val Ser  Leu Val Ala 
    1055                 1060                 1065             


Ile Lys  Gly Asn Gln Glu Ser  Pro Lys Ala Thr Gly  Val Phe Thr 
    1070                 1075                 1080             


Thr Leu  Gln Pro Gly Ser Ser  Ile Pro Pro Tyr Asn  Thr Glu Val 
    1085                 1090                 1095             


Thr Glu  Thr Thr Ile Val Ile  Thr Trp Thr Pro Ala  Pro Arg Ile 
    1100                 1105                 1110             


Gly Phe  Lys Leu Gly Val Arg  Pro Ser Gln Gly Gly  Glu Ala Pro 
    1115                 1120                 1125             


Arg Glu  Val Thr Ser Asp Ser  Gly Ser Ile Val Val  Ser Gly Leu 
    1130                 1135                 1140             


Thr Pro  Gly Val Glu Tyr Val  Tyr Thr Ile Gln Val  Leu Arg Asp 
    1145                 1150                 1155             


Gly Gln  Glu Arg Asp Ala Pro  Ile Val Asn Lys Val  Val Thr Pro 
    1160                 1165                 1170             


Leu Ser  Pro Pro Thr Asn Leu  His Leu Glu Ala Asn  Pro Asp Thr 
    1175                 1180                 1185             


Gly Val  Leu Thr Val Ser Trp  Glu Arg Ser Thr Thr  Pro Asp Ile 
    1190                 1195                 1200             


Thr Gly  Tyr Arg Ile Thr Thr  Thr Pro Thr Asn Gly  Gln Gln Gly 
    1205                 1210                 1215             


Asn Ser  Leu Glu Glu Val Val  His Ala Asp Gln Ser  Ser Cys Thr 
    1220                 1225                 1230             


Phe Asp  Asn Leu Ser Pro Gly  Leu Glu Tyr Asn Val  Ser Val Tyr 
    1235                 1240                 1245             


Thr Val  Lys Asp Asp Lys Glu  Ser Val Pro Ile Ser  Asp Thr Ile 
    1250                 1255                 1260             


Ile Pro  Ala Val Pro Pro Pro  Thr Asp Leu Arg Phe  Thr Asn Ile 
    1265                 1270                 1275             


Gly Pro  Asp Thr Met Arg Val  Thr Trp Ala Pro Pro  Pro Ser Ile 
    1280                 1285                 1290             


Asp Leu  Thr Asn Phe Leu Val  Arg Tyr Ser Pro Val  Lys Asn Glu 
    1295                 1300                 1305             


Glu Asp  Val Ala Glu Leu Ser  Ile Ser Pro Ser Asp  Asn Ala Val 
    1310                 1315                 1320             


Val Leu  Thr Asn Leu Leu Pro  Gly Thr Glu Tyr Val  Val Ser Val 
    1325                 1330                 1335             


Ser Ser  Val Tyr Glu Gln His  Glu Ser Thr Pro Leu  Arg Gly Arg 
    1340                 1345                 1350             


Gln Lys  Thr Gly Leu Asp Ser  Pro Thr Gly Ile Asp  Phe Ser Asp 
    1355                 1360                 1365             


Ile Thr  Ala Asn Ser Phe Thr  Val His Trp Ile Ala  Pro Arg Ala 
    1370                 1375                 1380             


Thr Ile  Thr Gly Tyr Arg Ile  Arg His His Pro Glu  His Phe Ser 
    1385                 1390                 1395             


Gly Arg  Pro Arg Glu Asp Arg  Val Pro His Ser Arg  Asn Ser Ile 
    1400                 1405                 1410             


Thr Leu  Thr Asn Leu Thr Pro  Gly Thr Glu Tyr Val  Val Ser Ile 
    1415                 1420                 1425             


Val Ala  Leu Asn Gly Arg Glu  Glu Ser Pro Leu Leu  Ile Gly Gln 
    1430                 1435                 1440             


Gln Ser  Thr Val Ser Asp Val  Pro Arg Asp Leu Glu  Val Val Ala 
    1445                 1450                 1455             


Ala Thr  Pro Thr Ser Leu Leu  Ile Ser Trp Asp Ala  Pro Ala Val 
    1460                 1465                 1470             


Thr Val  Arg Tyr Tyr Arg Ile  Thr Tyr Gly Glu Thr  Gly Gly Asn 
    1475                 1480                 1485             


Ser Pro  Val Gln Glu Phe Thr  Val Pro Gly Ser Lys  Ser Thr Ala 
    1490                 1495                 1500             


Thr Ile  Ser Gly Leu Lys Pro  Gly Val Asp Tyr Thr  Ile Thr Val 
    1505                 1510                 1515             


Tyr Ala  Val Thr Gly Arg Gly  Asp Ser Pro Ala Ser  Ser Lys Pro 
    1520                 1525                 1530             


Ile Ser  Ile Asn Tyr Arg Thr  Glu Ile Asp Lys Pro  Ser Gln Met 
    1535                 1540                 1545             


Gln Val  Thr Asp Val Gln Asp  Asn Ser Ile Ser Val  Lys Trp Leu 
    1550                 1555                 1560             


Pro Ser  Ser Ser Pro Val Thr  Gly Tyr Arg Val Thr  Thr Thr Pro 
    1565                 1570                 1575             


Lys Asn  Gly Pro Gly Pro Thr  Lys Thr Lys Thr Ala  Gly Pro Asp 
    1580                 1585                 1590             


Gln Thr  Glu Met Thr Ile Glu  Gly Leu Gln Pro Thr  Val Glu Tyr 
    1595                 1600                 1605             


Val Val  Ser Val Tyr Ala Gln  Asn Pro Ser Gly Glu  Ser Gln Pro 
    1610                 1615                 1620             


Leu Val  Gln Thr Ala Val Thr  Asn Ile Asp Arg Pro  Lys Gly Leu 
    1625                 1630                 1635             


Ala Phe  Thr Asp Val Asp Val  Asp Ser Ile Lys Ile  Ala Trp Glu 
    1640                 1645                 1650             


Ser Pro  Gln Gly Gln Val Ser  Arg Tyr Arg Val Thr  Tyr Ser Ser 
    1655                 1660                 1665             


Pro Glu  Asp Gly Ile His Glu  Leu Phe Pro Ala Pro  Asp Gly Glu 
    1670                 1675                 1680             


Glu Asp  Thr Ala Glu Leu Gln  Gly Leu Arg Pro Gly  Ser Glu Tyr 
    1685                 1690                 1695             


Thr Val  Ser Val Val Ala Leu  His Asp Asp Met Glu  Ser Gln Pro 
    1700                 1705                 1710             


Leu Ile  Gly Thr Gln Ser Thr  Ala Ile Pro Ala Pro  Thr Asp Leu 
    1715                 1720                 1725             


Lys Phe  Thr Gln Val Thr Pro  Thr Ser Leu Ser Ala  Gln Trp Thr 
    1730                 1735                 1740             


Pro Pro  Asn Val Gln Leu Thr  Gly Tyr Arg Val Arg  Val Thr Pro 
    1745                 1750                 1755             


Lys Glu  Lys Thr Gly Pro Met  Lys Glu Ile Asn Leu  Ala Pro Asp 
    1760                 1765                 1770             


Ser Ser  Ser Val Val Val Ser  Gly Leu Met Val Ala  Thr Lys Tyr 
    1775                 1780                 1785             


Glu Val  Ser Val Tyr Ala Leu  Lys Asp Thr Leu Thr  Ser Arg Pro 
    1790                 1795                 1800             


Ala Gln  Gly Val Val Thr Thr  Leu Glu Asn Val Ser  Pro Pro Arg 
    1805                 1810                 1815             


Arg Ala  Arg Val Thr Asp Ala  Thr Glu Thr Thr Ile  Thr Ile Ser 
    1820                 1825                 1830             


Trp Arg  Thr Lys Thr Glu Thr  Ile Thr Gly Phe Gln  Val Asp Ala 
    1835                 1840                 1845             


Val Pro  Ala Asn Gly Gln Thr  Pro Ile Gln Arg Thr  Ile Lys Pro 
    1850                 1855                 1860             


Asp Val  Arg Ser Tyr Thr Ile  Thr Gly Leu Gln Pro  Gly Thr Asp 
    1865                 1870                 1875             


Tyr Lys  Ile Tyr Leu Tyr Thr  Leu Asn Asp Asn Ala  Arg Ser Ser 
    1880                 1885                 1890             


Pro Val  Val Ile Asp Ala Ser  Thr Ala Ile Asp Ala  Pro Ser Asn 
    1895                 1900                 1905             


Leu Arg  Phe Leu Ala Thr Thr  Pro Asn Ser Leu Leu  Val Ser Trp 
    1910                 1915                 1920             


Gln Pro  Pro Arg Ala Arg Ile  Thr Gly Tyr Ile Ile  Lys Tyr Glu 
    1925                 1930                 1935             


Lys Pro  Gly Ser Pro Pro Arg  Glu Val Val Pro Arg  Pro Arg Pro 
    1940                 1945                 1950             


Gly Val  Thr Glu Ala Thr Ile  Thr Gly Leu Glu Pro  Gly Thr Glu 
    1955                 1960                 1965             


Tyr Thr  Ile Tyr Val Ile Ala  Leu Lys Asn Asn Gln  Lys Ser Glu 
    1970                 1975                 1980             


Pro Leu  Ile Gly Arg Lys Lys  Thr Asp Glu Leu Pro  Gln Leu Val 
    1985                 1990                 1995             


Thr Leu  Pro His Pro Asn Leu  His Gly Pro Glu Ile  Leu Asp Val 
    2000                 2005                 2010             


Pro Ser  Thr Val Gln Lys Thr  Pro Phe Val Thr His  Pro Gly Tyr 
    2015                 2020                 2025             


Asp Thr  Gly Asn Gly Ile Gln  Leu Pro Gly Thr Ser  Gly Gln Gln 
    2030                 2035                 2040             


Pro Ser  Val Gly Gln Gln Met  Ile Phe Glu Glu His  Gly Phe Arg 
    2045                 2050                 2055             


Arg Thr  Thr Pro Pro Thr Thr  Ala Thr Pro Ile Arg  His Arg Pro 
    2060                 2065                 2070             


Arg Pro  Tyr Pro Pro Asn Val  Gly Glu Glu Ile Gln  Ile Gly His 
    2075                 2080                 2085             


Ile Pro  Arg Glu Asp Val Asp  Tyr His Leu Tyr Pro  His Gly Pro 
    2090                 2095                 2100             


Gly Leu  Asn Pro Asn Ala Ser  Thr Gly Gln Glu Ala  Leu Ser Gln 
    2105                 2110                 2115             


Thr Thr  Ile Ser Trp Ala Pro  Phe Gln Asp Thr Ser  Glu Tyr Ile 
    2120                 2125                 2130             


Ile Ser  Cys His Pro Val Gly  Thr Asp Glu Glu Pro  Leu Gln Phe 
    2135                 2140                 2145             


Arg Val  Pro Gly Thr Ser Thr  Ser Ala Thr Leu Thr  Gly Leu Thr 
    2150                 2155                 2160             


Arg Gly  Ala Thr Tyr Asn Val  Ile Val Glu Ala Leu  Lys Asp Gln 
    2165                 2170                 2175             


Gln Arg  His Lys Val Arg Glu  Glu Val Val Thr Val  Gly Asn Ser 
    2180                 2185                 2190             


Val Asn  Glu Gly Leu Asn Gln  Pro Thr Asp Asp Ser  Cys Phe Asp 
    2195                 2200                 2205             


Pro Tyr  Thr Val Ser His Tyr  Ala Val Gly Asp Glu  Trp Glu Arg 
    2210                 2215                 2220             


Met Ser  Glu Ser Gly Phe Lys  Leu Leu Cys Gln Cys  Leu Gly Phe 
    2225                 2230                 2235             


Gly Ser  Gly His Phe Arg Cys  Asp Ser Ser Arg Trp  Cys His Asp 
    2240                 2245                 2250             


Asn Gly  Val Asn Tyr Lys Ile  Gly Glu Lys Trp Asp  Arg Gln Gly 
    2255                 2260                 2265             


Glu Asn  Gly Gln Met Met Ser  Cys Thr Cys Leu Gly  Asn Gly Lys 
    2270                 2275                 2280             


Gly Glu  Phe Lys Cys Asp Pro  His Glu Ala Thr Cys  Tyr Asp Asp 
    2285                 2290                 2295             


Gly Lys  Thr Tyr His Val Gly  Glu Gln Trp Gln Lys  Glu Tyr Leu 
    2300                 2305                 2310             


Gly Ala  Ile Cys Ser Cys Thr  Cys Phe Gly Gly Gln  Arg Gly Trp 
    2315                 2320                 2325             


Arg Cys  Asp Asn Cys Arg Arg  Pro Gly Gly Glu Pro  Ser Pro Glu 
    2330                 2335                 2340             


Gly Thr  Thr Gly Gln Ser Tyr  Asn Gln Tyr Ser Gln  Arg Tyr His 
    2345                 2350                 2355             


Gln Arg  Thr Asn Thr Asn Val  Asn Cys Pro Ile Glu  Cys Phe Met 
    2360                 2365                 2370             


Pro Leu  Asp Val Gln Ala Asp  Arg Glu Asp Ser Arg  Glu 
    2375                 2380                 2385     


