                         SEQUENCE LISTING

<110>  Neutrolis, Inc.
       Prasanna, Angelene
       Fuchs, Tobias
 
<120>  DNASE 1-LIKE 2 ENGINEERED FOR MANUFACTURING AND USE IN THERAPY

<130>  NTR-009PC/119601-5009

<140>  PCT/US2022/016771
<141>  2022-04-15

<150>  US 63/150,433
<151>  2021-02-17

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  299
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gly Gly Pro Arg Ala Leu Leu Ala Ala Leu Trp Ala Leu Glu Ala 
1               5                   10                  15      


Ala Gly Thr Ala Ala Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe 
            20                  25                  30          


Gly Asp Ser Lys Val Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys 
        35                  40                  45              


Ile Leu Ala Gly Tyr Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro 
    50                  55                  60                  


Asp Leu Ser Ala Val Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser 
65                  70                  75                  80  


Glu His Glu Tyr Ser Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln 
                85                  90                  95      


Tyr Lys Glu Met Tyr Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val 
            100                 105                 110         


Val Asp Thr Tyr Leu Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu 
        115                 120                 125             


Pro Phe Val Val Lys Phe Ser Ala Pro Gly Thr Gly Glu Arg Ala Pro 
    130                 135                 140                 


Pro Leu Pro Ser Arg Arg Ala Leu Thr Pro Pro Pro Leu Pro Ala Ala 
145                 150                 155                 160 


Ala Gln Asn Leu Val Leu Ile Pro Leu His Ala Ala Pro His Gln Ala 
                165                 170                 175     


Val Ala Glu Ile Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Ile Asp 
            180                 185                 190         


Lys Trp Gly Thr Asp Asp Met Leu Phe Leu Gly Asp Phe Asn Ala Asp 
        195                 200                 205             


Cys Ser Tyr Val Arg Ala Gln Asp Trp Ala Ala Ile Arg Leu Arg Ser 
    210                 215                 220                 


Ser Glu Val Phe Lys Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val 
225                 230                 235                 240 


Gly Asn Ser Asp Cys Ala Tyr Asp Arg Ile Val Ala Cys Gly Ala Arg 
                245                 250                 255     


Leu Arg Arg Ser Leu Lys Pro Gln Ser Ala Thr Val His Asp Phe Gln 
            260                 265                 270         


Glu Glu Phe Gly Leu Asp Gln Thr Gln Ala Leu Ala Ile Ser Asp His 
        275                 280                 285             


Phe Pro Val Glu Val Thr Leu Lys Phe His Arg 
    290                 295                 


<210>  2
<211>  278
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Gly Pro Arg Ala Leu Leu Ala Ala Leu Trp Ala Leu Glu Ala 
1               5                   10                  15      


Ala Gly Thr Ala Ala Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe 
            20                  25                  30          


Gly Asp Ser Lys Val Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys 
        35                  40                  45              


Ile Leu Ala Gly Tyr Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro 
    50                  55                  60                  


Asp Leu Ser Ala Val Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser 
65                  70                  75                  80  


Glu His Glu Tyr Ser Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln 
                85                  90                  95      


Tyr Lys Glu Met Tyr Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val 
            100                 105                 110         


Val Asp Thr Tyr Leu Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu 
        115                 120                 125             


Pro Phe Val Val Lys Phe Ser Ala Pro Gly Thr Ala Gln Asn Leu Val 
    130                 135                 140                 


Leu Ile Pro Leu His Ala Ala Pro His Gln Ala Val Ala Glu Ile Asp 
145                 150                 155                 160 


Ala Leu Tyr Asp Val Tyr Leu Asp Val Ile Asp Lys Trp Gly Thr Asp 
                165                 170                 175     


Asp Met Leu Phe Leu Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Arg 
            180                 185                 190         


Ala Gln Asp Trp Ala Ala Ile Arg Leu Arg Ser Ser Glu Val Phe Lys 
        195                 200                 205             


Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Gly Asn Ser Asp Cys 
    210                 215                 220                 


Ala Tyr Asp Arg Ile Val Ala Cys Gly Ala Arg Leu Arg Arg Ser Leu 
225                 230                 235                 240 


Lys Pro Gln Ser Ala Thr Val His Asp Phe Gln Glu Glu Phe Gly Leu 
                245                 250                 255     


Asp Gln Thr Gln Ala Leu Ala Ile Ser Asp His Phe Pro Val Glu Val 
            260                 265                 270         


Thr Leu Lys Phe His Arg 
        275             


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  3

Gly Glu Arg Ala Pro Pro Leu Pro Ser Arg Arg Ala Leu Thr Pro Pro 
1               5                   10                  15      


Pro Leu Pro Ala Ala 
            20      


<210>  4
<211>  278
<212>  PRT
<213>  Homo sapiens

<400>  4

Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe Gly Asp Ser Lys Val 
1               5                   10                  15      


Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys Ile Leu Ala Gly Tyr 
            20                  25                  30          


Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro Asp Leu Ser Ala Val 
        35                  40                  45              


Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser Glu His Glu Tyr Ser 
    50                  55                  60                  


Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln Tyr Lys Glu Met Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val Val Asp Thr Tyr Leu 
                85                  90                  95      


Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu Pro Phe Val Val Lys 
            100                 105                 110         


Phe Ser Ala Pro Gly Thr Gly Glu Arg Ala Pro Pro Leu Pro Ser Arg 
        115                 120                 125             


Arg Ala Leu Thr Pro Pro Pro Leu Pro Ala Ala Ala Gln Asn Leu Val 
    130                 135                 140                 


Leu Ile Pro Leu His Ala Ala Pro His Gln Ala Val Ala Glu Ile Asp 
145                 150                 155                 160 


Ala Leu Tyr Asp Val Tyr Leu Asp Val Ile Asp Lys Trp Gly Thr Asp 
                165                 170                 175     


Asp Met Leu Phe Leu Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Arg 
            180                 185                 190         


Ala Gln Asp Trp Ala Ala Ile Arg Leu Arg Ser Ser Glu Val Phe Lys 
        195                 200                 205             


Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Gly Asn Ser Asp Cys 
    210                 215                 220                 


Ala Tyr Asp Arg Ile Val Ala Cys Gly Ala Arg Leu Arg Arg Ser Leu 
225                 230                 235                 240 


Lys Pro Gln Ser Ala Thr Val His Asp Phe Gln Glu Glu Phe Gly Leu 
                245                 250                 255     


Asp Gln Thr Gln Ala Leu Ala Ile Ser Asp His Phe Pro Val Glu Val 
            260                 265                 270         


Thr Leu Lys Phe His Arg 
        275             


<210>  5
<211>  277
<212>  PRT
<213>  Homo sapiens

<400>  5

Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe Gly Asp Ser Lys Val 
1               5                   10                  15      


Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys Ile Leu Ala Gly Tyr 
            20                  25                  30          


Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro Asp Leu Ser Ala Val 
        35                  40                  45              


Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser Glu His Glu Tyr Ser 
    50                  55                  60                  


Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln Tyr Lys Glu Met Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val Val Asp Thr Tyr Leu 
                85                  90                  95      


Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu Pro Phe Val Val Lys 
            100                 105                 110         


Phe Ser Ala Pro Gly Thr Gly Glu Arg Ala Pro Pro Leu Pro Ser Arg 
        115                 120                 125             


Arg Ala Leu Thr Pro Pro Pro Leu Pro Ala Ala Ala Gln Asn Leu Val 
    130                 135                 140                 


Leu Ile Pro Leu His Ala Ala Pro His Gln Ala Val Ala Glu Ile Asp 
145                 150                 155                 160 


Ala Leu Tyr Asp Val Tyr Leu Asp Val Ile Asp Lys Trp Gly Thr Asp 
                165                 170                 175     


Asp Met Leu Phe Leu Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Arg 
            180                 185                 190         


Ala Gln Asp Trp Ala Ala Ile Arg Leu Arg Ser Ser Glu Val Phe Lys 
        195                 200                 205             


Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Gly Asn Ser Asp Cys 
    210                 215                 220                 


Ala Tyr Asp Arg Ile Val Ala Cys Gly Ala Arg Leu Arg Arg Ser Leu 
225                 230                 235                 240 


Lys Pro Gln Ser Ala Thr Val His Asp Phe Gln Glu Glu Phe Gly Leu 
                245                 250                 255     


Asp Gln Thr Gln Ala Leu Ala Ile Ser Asp His Phe Pro Val Glu Val 
            260                 265                 270         


Thr Leu Lys Phe His 
        275         


<210>  6
<211>  257
<212>  PRT
<213>  Homo sapiens

<400>  6

Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe Gly Asp Ser Lys Val 
1               5                   10                  15      


Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys Ile Leu Ala Gly Tyr 
            20                  25                  30          


Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro Asp Leu Ser Ala Val 
        35                  40                  45              


Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser Glu His Glu Tyr Ser 
    50                  55                  60                  


Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln Tyr Lys Glu Met Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val Val Asp Thr Tyr Leu 
                85                  90                  95      


Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu Pro Phe Val Val Lys 
            100                 105                 110         


Phe Ser Ala Pro Gly Thr Ala Gln Asn Leu Val Leu Ile Pro Leu His 
        115                 120                 125             


Ala Ala Pro His Gln Ala Val Ala Glu Ile Asp Ala Leu Tyr Asp Val 
    130                 135                 140                 


Tyr Leu Asp Val Ile Asp Lys Trp Gly Thr Asp Asp Met Leu Phe Leu 
145                 150                 155                 160 


Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Arg Ala Gln Asp Trp Ala 
                165                 170                 175     


Ala Ile Arg Leu Arg Ser Ser Glu Val Phe Lys Trp Leu Ile Pro Asp 
            180                 185                 190         


Ser Ala Asp Thr Thr Val Gly Asn Ser Asp Cys Ala Tyr Asp Arg Ile 
        195                 200                 205             


Val Ala Cys Gly Ala Arg Leu Arg Arg Ser Leu Lys Pro Gln Ser Ala 
    210                 215                 220                 


Thr Val His Asp Phe Gln Glu Glu Phe Gly Leu Asp Gln Thr Gln Ala 
225                 230                 235                 240 


Leu Ala Ile Ser Asp His Phe Pro Val Glu Val Thr Leu Lys Phe His 
                245                 250                 255     


Arg 
    


<210>  7
<211>  256
<212>  PRT
<213>  Homo sapiens

<400>  7

Leu Arg Ile Gly Ala Phe Asn Ile Gln Ser Phe Gly Asp Ser Lys Val 
1               5                   10                  15      


Ser Asp Pro Ala Cys Gly Ser Ile Ile Ala Lys Ile Leu Ala Gly Tyr 
            20                  25                  30          


Asp Leu Ala Leu Val Gln Glu Val Arg Asp Pro Asp Leu Ser Ala Val 
        35                  40                  45              


Ser Ala Leu Met Glu Gln Ile Asn Ser Val Ser Glu His Glu Tyr Ser 
    50                  55                  60                  


Phe Val Ser Ser Gln Pro Leu Gly Arg Asp Gln Tyr Lys Glu Met Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Lys Asp Ala Val Ser Val Val Asp Thr Tyr Leu 
                85                  90                  95      


Tyr Pro Asp Pro Glu Asp Val Phe Ser Arg Glu Pro Phe Val Val Lys 
            100                 105                 110         


Phe Ser Ala Pro Gly Thr Ala Gln Asn Leu Val Leu Ile Pro Leu His 
        115                 120                 125             


Ala Ala Pro His Gln Ala Val Ala Glu Ile Asp Ala Leu Tyr Asp Val 
    130                 135                 140                 


Tyr Leu Asp Val Ile Asp Lys Trp Gly Thr Asp Asp Met Leu Phe Leu 
145                 150                 155                 160 


Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Arg Ala Gln Asp Trp Ala 
                165                 170                 175     


Ala Ile Arg Leu Arg Ser Ser Glu Val Phe Lys Trp Leu Ile Pro Asp 
            180                 185                 190         


Ser Ala Asp Thr Thr Val Gly Asn Ser Asp Cys Ala Tyr Asp Arg Ile 
        195                 200                 205             


Val Ala Cys Gly Ala Arg Leu Arg Arg Ser Leu Lys Pro Gln Ser Ala 
    210                 215                 220                 


Thr Val His Asp Phe Gln Glu Glu Phe Gly Leu Asp Gln Thr Gln Ala 
225                 230                 235                 240 


Leu Ala Ile Ser Asp His Phe Pro Val Glu Val Thr Leu Lys Phe His 
                245                 250                 255     


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  8

Met Gly Gly Pro Arg Ala Leu Leu Ala Ala Leu Trp Ala Leu Glu Ala 
1               5                   10                  15      


Ala Gly Thr Ala Ala 
            20      


<210>  9
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  9

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser 
    


<210>  10
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  10

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg 
            20                  


<210>  11
<211>  585
<212>  PRT
<213>  Homo sapiens

<400>  11

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  12

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  13
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  13

Met Arg Gly Met Lys Leu Leu Gly Ala Leu Leu Ala Leu Ala Ala Leu 
1               5                   10                  15      


Leu Gln Gly Ala Val Ser 
            20          


<210>  14
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  14

Met His Tyr Pro Thr Ala Leu Leu Phe Leu Ile Leu Ala Asn Gly Ala 
1               5                   10                  15      


Gln Ala 
        


<210>  15
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  15

Met Ser Arg Glu Leu Ala Pro Leu Leu Leu Leu Leu Leu Ser Ile His 
1               5                   10                  15      


Ser Ala Leu Ala 
            20  


