                         SEQUENCE LISTING

<110>  Vaxcyte, Inc.
 
<120>  PURIFICATION OF POLYSACCHARIDES AND POLYPEPTIDE CONJUGATES
       THEREOF

<130>  STRO-015/02WO 331540-2122

<150>  US 63/288,387
<151>  2021-12-10

<150>  US 63/150,516
<151>  2021-02-17

<160>  83    

<170>  PatentIn version 3.5

<210>  1
<211>  962
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C5a [90-1035 fragment] - WT w/ leader

<400>  1

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp 
            20                  25                  30          


Leu Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys 
        35                  40                  45              


Gly Ala Gly Thr Val Val Ala Val Ile Asp Ala Gly Phe Asp Lys Asn 
    50                  55                  60                  


His Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser 
65                  70                  75                  80  


Lys Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly 
                85                  90                  95      


Glu Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp 
            100                 105                 110         


Gly Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile 
        115                 120                 125             


Leu Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu 
    130                 135                 140                 


Gly Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val 
145                 150                 155                 160 


Asn Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp 
                165                 170                 175     


Ala Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala 
            180                 185                 190         


Ala Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp 
        195                 200                 205             


Tyr Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp 
    210                 215                 220                 


Ser Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp 
225                 230                 235                 240 


Tyr Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val 
                245                 250                 255     


Ala Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys 
            260                 265                 270         


Thr Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg 
        275                 280                 285             


Phe Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met 
    290                 295                 300                 


Lys Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu 
305                 310                 315                 320 


Arg Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala 
                325                 330                 335     


Gly Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro 
            340                 345                 350         


Ile Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg 
        355                 360                 365             


Lys Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe 
    370                 375                 380                 


Asn Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser 
385                 390                 395                 400 


Arg Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp 
                405                 410                 415     


Ile Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys 
            420                 425                 430         


Tyr Ala Lys Leu Ser Gly Thr Ser Met Ser Ala Pro Leu Val Ala Gly 
        435                 440                 445             


Ile Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met 
    450                 455                 460                 


Thr Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser 
465                 470                 475                 480 


Ala Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg 
                485                 490                 495     


Gln Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr 
            500                 505                 510         


Met Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn 
        515                 520                 525             


Asn Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser 
    530                 535                 540                 


Asp Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys 
545                 550                 555                 560 


Val Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr 
                565                 570                 575     


Ser Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr 
            580                 585                 590         


Val Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met 
        595                 600                 605             


Lys Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro 
    610                 615                 620                 


Thr Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp 
625                 630                 635                 640 


Phe Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp 
                645                 650                 655     


Gly Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu 
            660                 665                 670         


Asp Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala 
        675                 680                 685             


Leu Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu 
    690                 695                 700                 


Gly Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr 
705                 710                 715                 720 


Ile Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr 
                725                 730                 735     


Ile His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn 
            740                 745                 750         


Gly Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg 
        755                 760                 765             


Asn Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val 
    770                 775                 780                 


Val Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn 
785                 790                 795                 800 


Asp Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp 
                805                 810                 815     


Asp Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr 
            820                 825                 830         


Tyr Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His 
        835                 840                 845             


Thr Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr 
    850                 855                 860                 


Ser Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys 
865                 870                 875                 880 


Pro Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr 
                885                 890                 895     


Met Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp 
            900                 905                 910         


Gly Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr 
        915                 920                 925             


Val Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala 
    930                 935                 940                 


Gly Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser 
945                 950                 955                 960 


Asn Lys 
        


<210>  2
<211>  945
<212>  PRT
<213>  Streptococcus pyogenes

<400>  2

Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp Leu 
1               5                   10                  15      


Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys Gly 
            20                  25                  30          


Ala Gly Thr Val Val Ala Val Ile Asp Ala Gly Phe Asp Lys Asn His 
        35                  40                  45              


Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser Lys 
    50                  55                  60                  


Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly Glu 
65                  70                  75                  80  


Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp Gly 
                85                  90                  95      


Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile Leu 
            100                 105                 110         


Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu Gly 
        115                 120                 125             


Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val Asn 
    130                 135                 140                 


Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp Ala 
145                 150                 155                 160 


Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala Ala 
                165                 170                 175     


Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp Tyr 
            180                 185                 190         


Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp Ser 
        195                 200                 205             


Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp Tyr 
    210                 215                 220                 


Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val Ala 
225                 230                 235                 240 


Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys Thr 
                245                 250                 255     


Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg Phe 
            260                 265                 270         


Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met Lys 
        275                 280                 285             


Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu Arg 
    290                 295                 300                 


Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala Gly 
305                 310                 315                 320 


Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro Ile 
                325                 330                 335     


Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg Lys 
            340                 345                 350         


Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe Asn 
        355                 360                 365             


Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser Arg 
    370                 375                 380                 


Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp Ile 
385                 390                 395                 400 


Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys Tyr 
                405                 410                 415     


Ala Lys Leu Ser Gly Thr Ser Met Ser Ala Pro Leu Val Ala Gly Ile 
            420                 425                 430         


Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met Thr 
        435                 440                 445             


Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser Ala 
    450                 455                 460                 


Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg Gln 
465                 470                 475                 480 


Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr Met 
                485                 490                 495     


Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn Asn 
            500                 505                 510         


Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser Asp 
        515                 520                 525             


Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys Val 
    530                 535                 540                 


Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr Ser 
545                 550                 555                 560 


Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr Val 
                565                 570                 575     


Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met Lys 
            580                 585                 590         


Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro Thr 
        595                 600                 605             


Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp Phe 
    610                 615                 620                 


Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp Gly 
625                 630                 635                 640 


Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu Asp 
                645                 650                 655     


Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala Leu 
            660                 665                 670         


Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu Gly 
        675                 680                 685             


Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr Ile 
    690                 695                 700                 


Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr Ile 
705                 710                 715                 720 


His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn Gly 
                725                 730                 735     


Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg Asn 
            740                 745                 750         


Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val Val 
        755                 760                 765             


Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn Asp 
    770                 775                 780                 


Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp Asp 
785                 790                 795                 800 


Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr Tyr 
                805                 810                 815     


Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His Thr 
            820                 825                 830         


Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr Ser 
        835                 840                 845             


Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys Pro 
    850                 855                 860                 


Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr Met 
865                 870                 875                 880 


Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp Gly 
                885                 890                 895     


Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr Val 
            900                 905                 910         


Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala Gly 
        915                 920                 925             


Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser Asn 
    930                 935                 940                 


Lys 
945 


<210>  3
<211>  962
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C5a [90-1035 fragment] D131A/S513A mutant w/ leader

<400>  3

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp 
            20                  25                  30          


Leu Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys 
        35                  40                  45              


Gly Ala Gly Thr Val Val Ala Val Ile Ala Ala Gly Phe Asp Lys Asn 
    50                  55                  60                  


His Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser 
65                  70                  75                  80  


Lys Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly 
                85                  90                  95      


Glu Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp 
            100                 105                 110         


Gly Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile 
        115                 120                 125             


Leu Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu 
    130                 135                 140                 


Gly Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val 
145                 150                 155                 160 


Asn Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp 
                165                 170                 175     


Ala Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala 
            180                 185                 190         


Ala Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp 
        195                 200                 205             


Tyr Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp 
    210                 215                 220                 


Ser Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp 
225                 230                 235                 240 


Tyr Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val 
                245                 250                 255     


Ala Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys 
            260                 265                 270         


Thr Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg 
        275                 280                 285             


Phe Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met 
    290                 295                 300                 


Lys Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu 
305                 310                 315                 320 


Arg Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala 
                325                 330                 335     


Gly Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro 
            340                 345                 350         


Ile Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg 
        355                 360                 365             


Lys Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe 
    370                 375                 380                 


Asn Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser 
385                 390                 395                 400 


Arg Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp 
                405                 410                 415     


Ile Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys 
            420                 425                 430         


Tyr Ala Lys Leu Ser Gly Thr Ala Met Ser Ala Pro Leu Val Ala Gly 
        435                 440                 445             


Ile Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met 
    450                 455                 460                 


Thr Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser 
465                 470                 475                 480 


Ala Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg 
                485                 490                 495     


Gln Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr 
            500                 505                 510         


Met Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn 
        515                 520                 525             


Asn Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser 
    530                 535                 540                 


Asp Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys 
545                 550                 555                 560 


Val Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr 
                565                 570                 575     


Ser Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr 
            580                 585                 590         


Val Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met 
        595                 600                 605             


Lys Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro 
    610                 615                 620                 


Thr Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp 
625                 630                 635                 640 


Phe Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp 
                645                 650                 655     


Gly Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu 
            660                 665                 670         


Asp Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala 
        675                 680                 685             


Leu Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu 
    690                 695                 700                 


Gly Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr 
705                 710                 715                 720 


Ile Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr 
                725                 730                 735     


Ile His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn 
            740                 745                 750         


Gly Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg 
        755                 760                 765             


Asn Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val 
    770                 775                 780                 


Val Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn 
785                 790                 795                 800 


Asp Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp 
                805                 810                 815     


Asp Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr 
            820                 825                 830         


Tyr Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His 
        835                 840                 845             


Thr Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr 
    850                 855                 860                 


Ser Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys 
865                 870                 875                 880 


Pro Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr 
                885                 890                 895     


Met Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp 
            900                 905                 910         


Gly Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr 
        915                 920                 925             


Val Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala 
    930                 935                 940                 


Gly Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser 
945                 950                 955                 960 


Asn Lys 
        


<210>  4
<211>  945
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C5a [90-1035 fragment] D131A/S513A mutant w/o leader

<400>  4

Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp Leu 
1               5                   10                  15      


Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys Gly 
            20                  25                  30          


Ala Gly Thr Val Val Ala Val Ile Ala Ala Gly Phe Asp Lys Asn His 
        35                  40                  45              


Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser Lys 
    50                  55                  60                  


Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly Glu 
65                  70                  75                  80  


Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp Gly 
                85                  90                  95      


Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile Leu 
            100                 105                 110         


Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu Gly 
        115                 120                 125             


Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val Asn 
    130                 135                 140                 


Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp Ala 
145                 150                 155                 160 


Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala Ala 
                165                 170                 175     


Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp Tyr 
            180                 185                 190         


Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp Ser 
        195                 200                 205             


Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp Tyr 
    210                 215                 220                 


Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val Ala 
225                 230                 235                 240 


Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys Thr 
                245                 250                 255     


Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg Phe 
            260                 265                 270         


Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met Lys 
        275                 280                 285             


Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu Arg 
    290                 295                 300                 


Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala Gly 
305                 310                 315                 320 


Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro Ile 
                325                 330                 335     


Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg Lys 
            340                 345                 350         


Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe Asn 
        355                 360                 365             


Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser Arg 
    370                 375                 380                 


Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp Ile 
385                 390                 395                 400 


Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys Tyr 
                405                 410                 415     


Ala Lys Leu Ser Gly Thr Ala Met Ser Ala Pro Leu Val Ala Gly Ile 
            420                 425                 430         


Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met Thr 
        435                 440                 445             


Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser Ala 
    450                 455                 460                 


Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg Gln 
465                 470                 475                 480 


Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr Met 
                485                 490                 495     


Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn Asn 
            500                 505                 510         


Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser Asp 
        515                 520                 525             


Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys Val 
    530                 535                 540                 


Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr Ser 
545                 550                 555                 560 


Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr Val 
                565                 570                 575     


Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met Lys 
            580                 585                 590         


Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro Thr 
        595                 600                 605             


Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp Phe 
    610                 615                 620                 


Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp Gly 
625                 630                 635                 640 


Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu Asp 
                645                 650                 655     


Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala Leu 
            660                 665                 670         


Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu Gly 
        675                 680                 685             


Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr Ile 
    690                 695                 700                 


Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr Ile 
705                 710                 715                 720 


His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn Gly 
                725                 730                 735     


Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg Asn 
            740                 745                 750         


Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val Val 
        755                 760                 765             


Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn Asp 
    770                 775                 780                 


Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp Asp 
785                 790                 795                 800 


Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr Tyr 
                805                 810                 815     


Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His Thr 
            820                 825                 830         


Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr Ser 
        835                 840                 845             


Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys Pro 
    850                 855                 860                 


Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr Met 
865                 870                 875                 880 


Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp Gly 
                885                 890                 895     


Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr Val 
            900                 905                 910         


Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala Gly 
        915                 920                 925             


Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser Asn 
    930                 935                 940                 


Lys 
945 


<210>  5
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO [79-571] fragment w/ leader

<400>  5

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
            20                  25                  30          


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
        35                  40                  45              


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
    50                  55                  60                  


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
65                  70                  75                  80  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
                85                  90                  95      


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
            100                 105                 110         


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
        115                 120                 125             


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
    130                 135                 140                 


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
145                 150                 155                 160 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
                165                 170                 175     


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
            180                 185                 190         


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
        195                 200                 205             


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
    210                 215                 220                 


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
225                 230                 235                 240 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
                245                 250                 255     


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
            260                 265                 270         


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
        275                 280                 285             


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
    290                 295                 300                 


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
305                 310                 315                 320 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
                325                 330                 335     


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
            340                 345                 350         


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
        355                 360                 365             


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
    370                 375                 380                 


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
385                 390                 395                 400 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
                405                 410                 415     


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
            420                 425                 430         


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
        435                 440                 445             


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
    450                 455                 460                 


Met Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Lys Val 
465                 470                 475                 480 


Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 
                485                 490                 495     


Ser Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
            500                 505                 510 


<210>  6
<211>  493
<212>  PRT
<213>  Streptococcus pyogenes

<400>  6

Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys Lys 
1               5                   10                  15      


Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile Asn 
            20                  25                  30          


Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala Lys 
        35                  40                  45              


Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys Lys 
    50                  55                  60                  


Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn Thr 
65                  70                  75                  80  


Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr Tyr 
                85                  90                  95      


Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys Pro 
            100                 105                 110         


Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp Leu 
        115                 120                 125             


Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr Tyr 
    130                 135                 140                 


Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His Asp 
145                 150                 155                 160 


Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr Glu 
                165                 170                 175     


Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val Asn 
            180                 185                 190         


Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile Ser 
        195                 200                 205             


Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe Tyr 
    210                 215                 220                 


Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp Lys 
225                 230                 235                 240 


Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu Ala 
                245                 250                 255     


Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe Val 
            260                 265                 270         


Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe Ser 
        275                 280                 285             


Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser Asp 
    290                 295                 300                 


Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala 
305                 310                 315                 320 


Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg Asn 
                325                 330                 335     


Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr Pro 
            340                 345                 350         


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly Val 
        355                 360                 365             


Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr Ser 
    370                 375                 380                 


Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr Glu 
385                 390                 395                 400 


Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val Ile 
                405                 410                 415     


Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro Phe 
            420                 425                 430         


Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile Met 
        435                 440                 445             


Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Lys Val Ile 
    450                 455                 460                 


Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile Ser 
465                 470                 475                 480 


Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
                485                 490             


<210>  7
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO [79-571 fragment] - W535 mutant w/ leader

<400>  7

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
            20                  25                  30          


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
        35                  40                  45              


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
    50                  55                  60                  


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
65                  70                  75                  80  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
                85                  90                  95      


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
            100                 105                 110         


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
        115                 120                 125             


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
    130                 135                 140                 


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
145                 150                 155                 160 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
                165                 170                 175     


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
            180                 185                 190         


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
        195                 200                 205             


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
    210                 215                 220                 


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
225                 230                 235                 240 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
                245                 250                 255     


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
            260                 265                 270         


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
        275                 280                 285             


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
    290                 295                 300                 


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
305                 310                 315                 320 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
                325                 330                 335     


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
            340                 345                 350         


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
        355                 360                 365             


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
    370                 375                 380                 


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
385                 390                 395                 400 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
                405                 410                 415     


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
            420                 425                 430         


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
        435                 440                 445             


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
    450                 455                 460                 


Met Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys Val 
465                 470                 475                 480 


Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 
                485                 490                 495     


Ser Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
            500                 505                 510 


<210>  8
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO [79-571 fragment] - W535 mutant w/o leader

<400>  8

Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys Lys 
1               5                   10                  15      


Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile Asn 
            20                  25                  30          


Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala Lys 
        35                  40                  45              


Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys Lys 
    50                  55                  60                  


Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn Thr 
65                  70                  75                  80  


Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr Tyr 
                85                  90                  95      


Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys Pro 
            100                 105                 110         


Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp Leu 
        115                 120                 125             


Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr Tyr 
    130                 135                 140                 


Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His Asp 
145                 150                 155                 160 


Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr Glu 
                165                 170                 175     


Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val Asn 
            180                 185                 190         


Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile Ser 
        195                 200                 205             


Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe Tyr 
    210                 215                 220                 


Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp Lys 
225                 230                 235                 240 


Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu Ala 
                245                 250                 255     


Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe Val 
            260                 265                 270         


Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe Ser 
        275                 280                 285             


Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser Asp 
    290                 295                 300                 


Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala 
305                 310                 315                 320 


Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg Asn 
                325                 330                 335     


Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr Pro 
            340                 345                 350         


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly Val 
        355                 360                 365             


Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr Ser 
    370                 375                 380                 


Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr Glu 
385                 390                 395                 400 


Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val Ile 
                405                 410                 415     


Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro Phe 
            420                 425                 430         


Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile Met 
        435                 440                 445             


Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys Val Ile 
    450                 455                 460                 


Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile Ser 
465                 470                 475                 480 


Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
                485                 490             


<210>  9
<211>  834
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] WT w/ leader

<400>  9

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
            20                  25                  30          


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
        35                  40                  45              


Lys Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
    50                  55                  60                  


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
65                  70                  75                  80  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
                85                  90                  95      


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
            100                 105                 110         


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
        115                 120                 125             


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
    130                 135                 140                 


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
145                 150                 155                 160 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
                165                 170                 175     


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
            180                 185                 190         


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
        195                 200                 205             


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
    210                 215                 220                 


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
225                 230                 235                 240 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
                245                 250                 255     


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Lys 
            260                 265                 270         


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
        275                 280                 285             


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
    290                 295                 300                 


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
305                 310                 315                 320 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
                325                 330                 335     


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
            340                 345                 350         


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
        355                 360                 365             


Tyr Lys Lys Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
    370                 375                 380                 


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
385                 390                 395                 400 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
                405                 410                 415     


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
            420                 425                 430         


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
        435                 440                 445             


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
    450                 455                 460                 


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
465                 470                 475                 480 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
                485                 490                 495     


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
            500                 505                 510         


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
        515                 520                 525             


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
    530                 535                 540                 


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
545                 550                 555                 560 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
                565                 570                 575     


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
            580                 585                 590         


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
        595                 600                 605             


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
    610                 615                 620                 


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
625                 630                 635                 640 


Lys Lys Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
                645                 650                 655     


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
            660                 665                 670         


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
        675                 680                 685             


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
    690                 695                 700                 


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
705                 710                 715                 720 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
                725                 730                 735     


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
            740                 745                 750         


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
        755                 760                 765             


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
    770                 775                 780                 


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
785                 790                 795                 800 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
                805                 810                 815     


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
            820                 825                 830         


Gln Arg 
        


<210>  10
<211>  817
<212>  PRT
<213>  Streptococcus pyogenes

<400>  10

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Lys 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Lys Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Lys Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu Leu 
                565                 570                 575     


Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp Gln 
            580                 585                 590         


Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr Glu 
        595                 600                 605             


Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala Lys 
    610                 615                 620                 


Lys Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn Arg 
625                 630                 635                 640 


Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu Ala 
                645                 650                 655     


Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala Leu 
            660                 665                 670         


Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu His 
        675                 680                 685             


Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg His 
    690                 695                 700                 


Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro Pro 
705                 710                 715                 720 


Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr Pro 
                725                 730                 735     


Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala Ser 
            740                 745                 750         


Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu Val 
        755                 760                 765             


Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys Ile 
    770                 775                 780                 


Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser Gly 
785                 790                 795                 800 


Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr Gln 
                805                 810                 815     


Arg 
    


<210>  11
<211>  834
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] nnAA (e.g. pAMF) K64/K287/K386/K657 
       mutant w/ leader


<220>
<221>  MOD_RES
<222>  (49)..(49)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (272)..(272)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (371)..(371)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (642)..(642)
<223>  Xaa = nnAA (e.g pAMF)

<400>  11

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
            20                  25                  30          


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
        35                  40                  45              


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
    50                  55                  60                  


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
65                  70                  75                  80  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
                85                  90                  95      


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
            100                 105                 110         


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
        115                 120                 125             


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
    130                 135                 140                 


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
145                 150                 155                 160 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
                165                 170                 175     


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
            180                 185                 190         


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
        195                 200                 205             


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
    210                 215                 220                 


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
225                 230                 235                 240 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
                245                 250                 255     


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
            260                 265                 270         


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
        275                 280                 285             


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
    290                 295                 300                 


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
305                 310                 315                 320 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
                325                 330                 335     


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
            340                 345                 350         


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
        355                 360                 365             


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
    370                 375                 380                 


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
385                 390                 395                 400 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
                405                 410                 415     


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
            420                 425                 430         


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
        435                 440                 445             


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
    450                 455                 460                 


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
465                 470                 475                 480 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
                485                 490                 495     


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
            500                 505                 510         


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
        515                 520                 525             


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
    530                 535                 540                 


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
545                 550                 555                 560 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
                565                 570                 575     


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
            580                 585                 590         


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
        595                 600                 605             


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
    610                 615                 620                 


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
625                 630                 635                 640 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
                645                 650                 655     


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
            660                 665                 670         


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
        675                 680                 685             


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
    690                 695                 700                 


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
705                 710                 715                 720 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
                725                 730                 735     


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
            740                 745                 750         


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
        755                 760                 765             


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
    770                 775                 780                 


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
785                 790                 795                 800 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
                805                 810                 815     


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
            820                 825                 830         


Gln Arg 
        


<210>  12
<211>  817
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] nnAA (e.g. pAMF)  K64/K287/K386/K657 
       mutant w/o leader


<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MOD_RES
<222>  (255)..(255)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (354)..(354)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (625)..(625)
<223>  Xaa = nnAA (e.g pAMF)

<400>  12

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Xaa 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu Leu 
                565                 570                 575     


Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp Gln 
            580                 585                 590         


Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr Glu 
        595                 600                 605             


Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala Lys 
    610                 615                 620                 


Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn Arg 
625                 630                 635                 640 


Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu Ala 
                645                 650                 655     


Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala Leu 
            660                 665                 670         


Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu His 
        675                 680                 685             


Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg His 
    690                 695                 700                 


Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro Pro 
705                 710                 715                 720 


Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr Pro 
                725                 730                 735     


Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala Ser 
            740                 745                 750         


Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu Val 
        755                 760                 765             


Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys Ile 
    770                 775                 780                 


Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser Gly 
785                 790                 795                 800 


Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr Gln 
                805                 810                 815     


Arg 
    


<210>  13
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] WT w/ leader

<400>  13

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
            20                  25                  30          


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
        35                  40                  45              


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
    50                  55                  60                  


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
65                  70                  75                  80  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
                85                  90                  95      


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
            100                 105                 110         


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
        115                 120                 125             


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
    130                 135                 140                 


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
145                 150                 155                 160 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
                165                 170                 175     


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
            180                 185                 190         


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
        195                 200                 205             


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
    210                 215                 220                 


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
225                 230                 235                 240 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
                245                 250                 255     


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
            260                 265                 270         


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
        275                 280                 285             


Phe Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
    290                 295                 300                 


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
305                 310                 315                 320 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala 
                325                 330                 335     


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
            340                 345                 350         


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
        355                 360                 365             


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
    370                 375                 380                 


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
385                 390                 395                 400 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
                405                 410                 415     


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
            420                 425         


<210>  14
<211>  410
<212>  PRT
<213>  Streptococcus pyogenes

<400>  14

Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu Lys 
1               5                   10                  15      


Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met Pro 
            20                  25                  30          


Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu Asp 
        35                  40                  45              


Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val Thr 
65                  70                  75                  80  


Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala Asn 
                85                  90                  95      


Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met Ala 
            100                 105                 110         


Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val Gln 
        115                 120                 125             


Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr Asp 
    130                 135                 140                 


Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn Leu 
145                 150                 155                 160 


Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser Leu 
                165                 170                 175     


Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly Lys 
            180                 185                 190         


Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro Met 
        195                 200                 205             


Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu Leu 
    210                 215                 220                 


Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr Asp 
225                 230                 235                 240 


Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn Leu 
                245                 250                 255     


Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys Phe 
            260                 265                 270         


Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe Thr 
        275                 280                 285             


Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr Tyr 
    290                 295                 300                 


Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala Glu 
305                 310                 315                 320 


Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg Cys 
                325                 330                 335     


Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp Gly 
            340                 345                 350         


Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn Arg 
        355                 360                 365             


Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu Ile 
    370                 375                 380                 


Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg Cys 
385                 390                 395                 400 


Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410 


<210>  15
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] D277A mut w/ leader

<400>  15

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
            20                  25                  30          


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
        35                  40                  45              


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
    50                  55                  60                  


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
65                  70                  75                  80  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
                85                  90                  95      


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
            100                 105                 110         


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
        115                 120                 125             


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
    130                 135                 140                 


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
145                 150                 155                 160 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
                165                 170                 175     


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
            180                 185                 190         


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
        195                 200                 205             


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
    210                 215                 220                 


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
225                 230                 235                 240 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
                245                 250                 255     


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
            260                 265                 270         


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
        275                 280                 285             


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
    290                 295                 300                 


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
305                 310                 315                 320 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala 
                325                 330                 335     


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
            340                 345                 350         


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
        355                 360                 365             


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
    370                 375                 380                 


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
385                 390                 395                 400 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
                405                 410                 415     


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
            420                 425         


<210>  16
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] D277A mut w/o leader

<400>  16

Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu Lys 
1               5                   10                  15      


Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met Pro 
            20                  25                  30          


Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu Asp 
        35                  40                  45              


Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val Thr 
65                  70                  75                  80  


Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala Asn 
                85                  90                  95      


Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met Ala 
            100                 105                 110         


Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val Gln 
        115                 120                 125             


Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr Asp 
    130                 135                 140                 


Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn Leu 
145                 150                 155                 160 


Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser Leu 
                165                 170                 175     


Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly Lys 
            180                 185                 190         


Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro Met 
        195                 200                 205             


Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu Leu 
    210                 215                 220                 


Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr Asp 
225                 230                 235                 240 


Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn Leu 
                245                 250                 255     


Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys Phe 
            260                 265                 270         


Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe Thr 
        275                 280                 285             


Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr Tyr 
    290                 295                 300                 


Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala Glu 
305                 310                 315                 320 


Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg Cys 
                325                 330                 335     


Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp Gly 
            340                 345                 350         


Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn Arg 
        355                 360                 365             


Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu Ile 
    370                 375                 380                 


Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg Cys 
385                 390                 395                 400 


Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410 


<210>  17
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] WT nnAA (e.g. pAMF) K15/K193/K316 w/ leader


<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (209)..(209)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (332)..(332)
<223>  Xaa = nnAA (e.g pAMF)

<400>  17

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu 
            20                  25                  30          


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
        35                  40                  45              


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
    50                  55                  60                  


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
65                  70                  75                  80  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
                85                  90                  95      


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
            100                 105                 110         


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
        115                 120                 125             


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
    130                 135                 140                 


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
145                 150                 155                 160 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
                165                 170                 175     


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
            180                 185                 190         


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
        195                 200                 205             


Xaa Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
    210                 215                 220                 


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
225                 230                 235                 240 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
                245                 250                 255     


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
            260                 265                 270         


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
        275                 280                 285             


Phe Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
    290                 295                 300                 


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
305                 310                 315                 320 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
                325                 330                 335     


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
            340                 345                 350         


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
        355                 360                 365             


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
    370                 375                 380                 


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
385                 390                 395                 400 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
                405                 410                 415     


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
            420                 425         


<210>  18
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] WT nnAA (e.g. pAMF) K15/K193/K316 w/o leader


<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (192)..(192)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (315)..(315)
<223>  Xaa = nnAA (e.g pAMF)

<400>  18

Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu Lys 
1               5                   10                  15      


Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met Pro 
            20                  25                  30          


Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu Asp 
        35                  40                  45              


Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val Thr 
65                  70                  75                  80  


Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala Asn 
                85                  90                  95      


Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met Ala 
            100                 105                 110         


Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val Gln 
        115                 120                 125             


Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr Asp 
    130                 135                 140                 


Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn Leu 
145                 150                 155                 160 


Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser Leu 
                165                 170                 175     


Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly Xaa 
            180                 185                 190         


Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro Met 
        195                 200                 205             


Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu Leu 
    210                 215                 220                 


Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr Asp 
225                 230                 235                 240 


Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn Leu 
                245                 250                 255     


Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys Phe 
            260                 265                 270         


Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe Thr 
        275                 280                 285             


Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr Tyr 
    290                 295                 300                 


Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala Glu 
305                 310                 315                 320 


Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg Cys 
                325                 330                 335     


Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp Gly 
            340                 345                 350         


Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn Arg 
        355                 360                 365             


Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu Ile 
    370                 375                 380                 


Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg Cys 
385                 390                 395                 400 


Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410 


<210>  19
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] D277A nnAA (e.g. pAMF) K15/K193/K316 w/ leader


<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (209)..(209)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (332)..(332)
<223>  Xaa = nnAA (e.g pAMF)

<400>  19

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu 
            20                  25                  30          


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
        35                  40                  45              


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
    50                  55                  60                  


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
65                  70                  75                  80  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
                85                  90                  95      


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
            100                 105                 110         


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
        115                 120                 125             


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
    130                 135                 140                 


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
145                 150                 155                 160 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
                165                 170                 175     


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
            180                 185                 190         


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
        195                 200                 205             


Xaa Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
    210                 215                 220                 


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
225                 230                 235                 240 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
                245                 250                 255     


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
            260                 265                 270         


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
        275                 280                 285             


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
    290                 295                 300                 


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
305                 310                 315                 320 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
                325                 330                 335     


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
            340                 345                 350         


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
        355                 360                 365             


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
    370                 375                 380                 


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
385                 390                 395                 400 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
                405                 410                 415     


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
            420                 425         


<210>  20
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] D277A nnAA (e.g. pAMF) K15/K193/K316 w/o leader


<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (192)..(192)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (315)..(315)
<223>  Xaa = nnAA (e.g pAMF)

<400>  20

Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu Lys 
1               5                   10                  15      


Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met Pro 
            20                  25                  30          


Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu Asp 
        35                  40                  45              


Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val Thr 
65                  70                  75                  80  


Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala Asn 
                85                  90                  95      


Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met Ala 
            100                 105                 110         


Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val Gln 
        115                 120                 125             


Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr Asp 
    130                 135                 140                 


Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn Leu 
145                 150                 155                 160 


Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser Leu 
                165                 170                 175     


Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly Xaa 
            180                 185                 190         


Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro Met 
        195                 200                 205             


Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu Leu 
    210                 215                 220                 


Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr Asp 
225                 230                 235                 240 


Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn Leu 
                245                 250                 255     


Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys Phe 
            260                 265                 270         


Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe Thr 
        275                 280                 285             


Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr Tyr 
    290                 295                 300                 


Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala Glu 
305                 310                 315                 320 


Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg Cys 
                325                 330                 335     


Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp Gly 
            340                 345                 350         


Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn Arg 
        355                 360                 365             


Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu Ile 
    370                 375                 380                 


Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg Cys 
385                 390                 395                 400 


Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410 


<210>  21
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFerritin [full length] WT w/ leader

<400>  21

Met His His His His His His Gly Ser Gly Ser Ser Gln Ile Arg Gln 
1               5                   10                  15      


Asn Tyr Ser Thr Asp Val Glu Ala Ala Val Asn Ser Leu Val Asn Leu 
            20                  25                  30          


Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser Leu Gly Phe Tyr Phe Asp 
        35                  40                  45              


Arg Asp Asp Val Ala Leu Glu Gly Val Ser His Phe Phe Arg Glu Leu 
    50                  55                  60                  


Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg Leu Leu Lys Met Gln Asn 
65                  70                  75                  80  


Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp Ile Lys Lys Pro Ala Glu 
                85                  90                  95      


Asp Glu Trp Gly Lys Thr Pro Asp Ala Met Lys Ala Ala Met Ala Leu 
            100                 105                 110         


Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp Leu His Ala Leu Gly Ser 
        115                 120                 125             


Ala Arg Thr Asp Pro His Leu Cys Asp Phe Leu Glu Thr His Phe Leu 
    130                 135                 140                 


Asp Glu Glu Val Lys Leu Ile Lys Lys Met Gly Asp His Leu Thr Asn 
145                 150                 155                 160 


Leu His Arg Leu Gly Gly Pro Glu Ala Gly Leu Gly Glu Tyr Leu Phe 
                165                 170                 175     


Glu Arg Leu Thr Leu Lys His Asp 
            180                 


<210>  22
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  22

Ser Ser Gln Ile Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala Val 
1               5                   10                  15      


Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser 
            20                  25                  30          


Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val Ser 
        35                  40                  45              


His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg 
    50                  55                  60                  


Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp 
65                  70                  75                  80  


Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala Met 
                85                  90                  95      


Lys Ala Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp 
            100                 105                 110         


Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp Phe 
        115                 120                 125             


Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys Met 
    130                 135                 140                 


Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala Gly 
145                 150                 155                 160 


Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Lys His Asp 
                165                 170                 


<210>  23
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFerritin [full length] nnAA (e.g. pAMF) mutant I5 w/ leader


<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Xaa = nnAA (e.g pAMF)

<400>  23

Met His His His His His His Gly Ser Gly Ser Ser Gln Xaa Arg Gln 
1               5                   10                  15      


Asn Tyr Ser Thr Asp Val Glu Ala Ala Val Asn Ser Leu Val Asn Leu 
            20                  25                  30          


Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser Leu Gly Phe Tyr Phe Asp 
        35                  40                  45              


Arg Asp Asp Val Ala Leu Glu Gly Val Ser His Phe Phe Arg Glu Leu 
    50                  55                  60                  


Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg Leu Leu Lys Met Gln Asn 
65                  70                  75                  80  


Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp Ile Lys Lys Pro Ala Glu 
                85                  90                  95      


Asp Glu Trp Gly Lys Thr Pro Asp Ala Met Lys Ala Ala Met Ala Leu 
            100                 105                 110         


Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp Leu His Ala Leu Gly Ser 
        115                 120                 125             


Ala Arg Thr Asp Pro His Leu Cys Asp Phe Leu Glu Thr His Phe Leu 
    130                 135                 140                 


Asp Glu Glu Val Lys Leu Ile Lys Lys Met Gly Asp His Leu Thr Asn 
145                 150                 155                 160 


Leu His Arg Leu Gly Gly Pro Glu Ala Gly Leu Gly Glu Tyr Leu Phe 
                165                 170                 175     


Glu Arg Leu Thr Leu Lys His Asp 
            180                 


<210>  24
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFerritin [full length] nnAA (e.g. pAMF) mutant I5 w/o leader


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa = nnAA (e.g pAMF)

<400>  24

Ser Ser Gln Xaa Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala Val 
1               5                   10                  15      


Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser 
            20                  25                  30          


Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val Ser 
        35                  40                  45              


His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg 
    50                  55                  60                  


Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp 
65                  70                  75                  80  


Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala Met 
                85                  90                  95      


Lys Ala Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp 
            100                 105                 110         


Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp Phe 
        115                 120                 125             


Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys Met 
    130                 135                 140                 


Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala Gly 
145                 150                 155                 160 


Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Lys His Asp 
                165                 170                 


<210>  25
<211>  536
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eCRM - nnAA (e.g. pAMF) mutant


<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (213)..(213)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (245)..(245)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (265)..(265)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (386)..(386)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (527)..(527)
<223>  Xaa = nnAA (e.g pAMF)

<400>  25

Met Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Xaa Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Asn Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Xaa Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Xaa Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Xaa Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Xaa Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn 
385                 390                 395                 400 


Thr Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly 
                405                 410                 415     


His Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly 
            420                 425                 430         


Val Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys 
        435                 440                 445             


Thr His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala 
    450                 455                 460                 


Ile Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val 
465                 470                 475                 480 


Gly Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser 
                485                 490                 495     


Ser Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val 
            500                 505                 510         


Leu Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Xaa Leu 
        515                 520                 525             


Ser Leu Phe Phe Glu Ile Lys Ser 
    530                 535     


<210>  26
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI (mut2) [full length] D277A nnAA (e.g. pAMF) mutant w/ leader


<220>
<221>  MOD_RES
<222>  (139)..(139)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (263)..(263)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (332)..(332)
<223>  Xaa = nnAA (e.g pAMF)

<400>  26

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
            20                  25                  30          


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
        35                  40                  45              


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
    50                  55                  60                  


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
65                  70                  75                  80  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
                85                  90                  95      


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
            100                 105                 110         


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
        115                 120                 125             


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Xaa Thr Met Ala Gly Val 
    130                 135                 140                 


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
145                 150                 155                 160 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
                165                 170                 175     


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
            180                 185                 190         


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
        195                 200                 205             


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
    210                 215                 220                 


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
225                 230                 235                 240 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
                245                 250                 255     


Asp Ala Ala Ser Ile Glu Xaa Leu Leu Val Asn Ile Phe Lys Gln Asn 
            260                 265                 270         


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
        275                 280                 285             


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
    290                 295                 300                 


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
305                 310                 315                 320 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
                325                 330                 335     


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
            340                 345                 350         


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
        355                 360                 365             


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
    370                 375                 380                 


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
385                 390                 395                 400 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
                405                 410                 415     


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
            420                 425         


<210>  27
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI (mut2) [full length] D277A nnAA (e.g. pAMF) mutant w/o 
       leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (123)..(123)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (247)..(247)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (316)..(316)
<223>  Xaa = nnAA (e.g pAMF)

<400>  27

Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
1               5                   10                  15      


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
            20                  25                  30          


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
        35                  40                  45              


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
    50                  55                  60                  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
65                  70                  75                  80  


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
                85                  90                  95      


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
            100                 105                 110         


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Xaa Thr Met Ala Gly Val 
        115                 120                 125             


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
    130                 135                 140                 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
145                 150                 155                 160 


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
                165                 170                 175     


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
            180                 185                 190         


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
        195                 200                 205             


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
    210                 215                 220                 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
225                 230                 235                 240 


Asp Ala Ala Ser Ile Glu Xaa Leu Leu Val Asn Ile Phe Lys Gln Asn 
                245                 250                 255     


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
            260                 265                 270         


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
        275                 280                 285             


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
    290                 295                 300                 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
                325                 330                 335     


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
            340                 345                 350         


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
        355                 360                 365             


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
    370                 375                 380                 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
385                 390                 395                 400 


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410     


<210>  28
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sfb1 repeat region with leader

<400>  28

Met His His His His His His Gly Ser Gly Val Glu Thr Glu Asp Thr 
1               5                   10                  15      


Lys Glu Pro Gly Val Leu Met Gly Gly Gln Ser Glu Ser Val Glu Phe 
            20                  25                  30          


Thr Lys Asp Thr Gln Thr Gly Met Ser Gly Gln Thr Thr Pro Gln Val 
        35                  40                  45              


Glu Thr Glu Asp Thr Lys Glu Pro Gly Val Leu Met Gly Gly Gln Ser 
    50                  55                  60                  


Glu Ser Val Glu Phe Thr Lys Asp Thr Gln Thr Gly Met Ser Gly Gln 
65                  70                  75                  80  


Thr Ala Ser Gln Val Glu Thr Glu Asp Thr Lys Glu Pro Gly Val Leu 
                85                  90                  95      


Met Gly Gly Gln Ser Glu Ser Val Glu Phe Thr Lys Asp Thr Gln Thr 
            100                 105                 110         


Gly Met Ser Gly Gln Thr Thr Pro Gln Val Glu Thr Glu Asp Thr Lys 
        115                 120                 125             


Glu Pro Gly Val Leu Met Gly Gly Gln Ser Glu Ser Val Glu Phe Thr 
    130                 135                 140                 


Lys Asp Thr Gln Thr Gly Met Ser Gly Phe Ser Glu Thr 
145                 150                 155         


<210>  29
<211>  946
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C5a [90-1035 fragment] WT w/o leader, with G overhang from 
       cleavage

<400>  29

Gly Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp 
1               5                   10                  15      


Leu Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys 
            20                  25                  30          


Gly Ala Gly Thr Val Val Ala Val Ile Asp Ala Gly Phe Asp Lys Asn 
        35                  40                  45              


His Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser 
    50                  55                  60                  


Lys Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly 
65                  70                  75                  80  


Glu Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp 
                85                  90                  95      


Gly Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile 
            100                 105                 110         


Leu Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu 
        115                 120                 125             


Gly Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val 
    130                 135                 140                 


Asn Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp 
145                 150                 155                 160 


Ala Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala 
                165                 170                 175     


Ala Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp 
            180                 185                 190         


Tyr Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp 
        195                 200                 205             


Ser Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp 
    210                 215                 220                 


Tyr Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val 
225                 230                 235                 240 


Ala Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys 
                245                 250                 255     


Thr Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg 
            260                 265                 270         


Phe Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met 
        275                 280                 285             


Lys Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu 
    290                 295                 300                 


Arg Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala 
305                 310                 315                 320 


Gly Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro 
                325                 330                 335     


Ile Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg 
            340                 345                 350         


Lys Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe 
        355                 360                 365             


Asn Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser 
    370                 375                 380                 


Arg Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp 
385                 390                 395                 400 


Ile Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys 
                405                 410                 415     


Tyr Ala Lys Leu Ser Gly Thr Ser Met Ser Ala Pro Leu Val Ala Gly 
            420                 425                 430         


Ile Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met 
        435                 440                 445             


Thr Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser 
    450                 455                 460                 


Ala Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg 
465                 470                 475                 480 


Gln Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr 
                485                 490                 495     


Met Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn 
            500                 505                 510         


Asn Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser 
        515                 520                 525             


Asp Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys 
    530                 535                 540                 


Val Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr 
545                 550                 555                 560 


Ser Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr 
                565                 570                 575     


Val Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met 
            580                 585                 590         


Lys Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro 
        595                 600                 605             


Thr Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp 
    610                 615                 620                 


Phe Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp 
625                 630                 635                 640 


Gly Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu 
                645                 650                 655     


Asp Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala 
            660                 665                 670         


Leu Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu 
        675                 680                 685             


Gly Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr 
    690                 695                 700                 


Ile Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr 
705                 710                 715                 720 


Ile His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn 
                725                 730                 735     


Gly Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg 
            740                 745                 750         


Asn Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val 
        755                 760                 765             


Val Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn 
    770                 775                 780                 


Asp Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp 
785                 790                 795                 800 


Asp Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr 
                805                 810                 815     


Tyr Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His 
            820                 825                 830         


Thr Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr 
        835                 840                 845             


Ser Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys 
    850                 855                 860                 


Pro Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr 
865                 870                 875                 880 


Met Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp 
                885                 890                 895     


Gly Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr 
            900                 905                 910         


Val Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala 
        915                 920                 925             


Gly Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser 
    930                 935                 940                 


Asn Lys 
945     


<210>  30
<211>  946
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C5a [90-1035 fragment] D131A/S513A w/o leader, with G overhang 
       from cleavage

<400>  30

Gly Lys Thr Ala Asp Thr Pro Val Thr Ser Lys Ala Thr Ile Arg Asp 
1               5                   10                  15      


Leu Asn Asp Pro Ser Gln Val Lys Thr Leu Gln Glu Lys Ala Gly Lys 
            20                  25                  30          


Gly Ala Gly Thr Val Val Ala Val Ile Ala Ala Gly Phe Asp Lys Asn 
        35                  40                  45              


His Glu Ala Trp Arg Leu Thr Asp Lys Thr Lys Ala Arg Tyr Gln Ser 
    50                  55                  60                  


Lys Glu Asp Leu Glu Lys Ala Lys Lys Glu His Gly Ile Thr Tyr Gly 
65                  70                  75                  80  


Glu Trp Val Asn Asp Lys Val Ala Tyr Tyr His Asp Tyr Ser Lys Asp 
                85                  90                  95      


Gly Lys Thr Ala Val Asp Gln Glu His Gly Thr His Val Ser Gly Ile 
            100                 105                 110         


Leu Ser Gly Asn Ala Pro Ser Glu Thr Lys Glu Pro Tyr Arg Leu Glu 
        115                 120                 125             


Gly Ala Met Pro Glu Ala Gln Leu Leu Leu Met Arg Val Glu Ile Val 
    130                 135                 140                 


Asn Gly Leu Ala Asp Tyr Ala Arg Asn Tyr Ala Gln Ala Ile Arg Asp 
145                 150                 155                 160 


Ala Val Asn Leu Gly Ala Lys Val Ile Asn Met Ser Phe Gly Asn Ala 
                165                 170                 175     


Ala Leu Ala Tyr Ala Asn Leu Pro Asp Glu Thr Lys Lys Ala Phe Asp 
            180                 185                 190         


Tyr Ala Lys Ser Lys Gly Val Ser Ile Val Thr Ser Ala Gly Asn Asp 
        195                 200                 205             


Ser Ser Phe Gly Gly Lys Thr Arg Leu Pro Leu Ala Asp His Pro Asp 
    210                 215                 220                 


Tyr Gly Val Val Gly Thr Pro Ala Ala Ala Asp Ser Thr Leu Thr Val 
225                 230                 235                 240 


Ala Ser Tyr Ser Pro Asp Lys Gln Leu Thr Glu Thr Ala Thr Val Lys 
                245                 250                 255     


Thr Ala Asp Lys Gln Asp Lys Glu Met Pro Val Leu Ser Thr Asn Arg 
            260                 265                 270         


Phe Glu Pro Asn Lys Ala Tyr Asp Tyr Ala Tyr Ala Asn Arg Gly Met 
        275                 280                 285             


Lys Glu Asp Asp Phe Lys Asp Val Lys Gly Lys Ile Ala Leu Ile Glu 
    290                 295                 300                 


Arg Gly Asp Ile Asp Phe Lys Asp Lys Ile Ala Asn Ala Lys Lys Ala 
305                 310                 315                 320 


Gly Ala Val Gly Val Leu Ile Tyr Asp Asn Gln Asp Lys Gly Phe Pro 
                325                 330                 335     


Ile Glu Leu Pro Asn Val Asp Gln Met Pro Ala Ala Phe Ile Ser Arg 
            340                 345                 350         


Lys Asp Gly Leu Leu Leu Lys Asp Asn Pro Lys Lys Thr Ile Thr Phe 
        355                 360                 365             


Asn Ala Thr Pro Lys Val Leu Pro Thr Ala Ser Gly Thr Lys Leu Ser 
    370                 375                 380                 


Arg Phe Ser Ser Trp Gly Leu Thr Ala Asp Gly Asn Ile Lys Pro Asp 
385                 390                 395                 400 


Ile Ala Ala Pro Gly Gln Asp Ile Leu Ser Ser Val Ala Asn Asn Lys 
                405                 410                 415     


Tyr Ala Lys Leu Ser Gly Thr Ala Met Ser Ala Pro Leu Val Ala Gly 
            420                 425                 430         


Ile Met Gly Leu Leu Gln Lys Gln Tyr Glu Thr Gln Tyr Pro Asp Met 
        435                 440                 445             


Thr Pro Ser Glu Arg Leu Asp Leu Ala Lys Lys Val Leu Met Ser Ser 
    450                 455                 460                 


Ala Thr Ala Leu Tyr Asp Glu Asp Glu Lys Ala Tyr Phe Ser Pro Arg 
465                 470                 475                 480 


Gln Gln Gly Ala Gly Ala Val Asp Ala Lys Lys Ala Ser Ala Ala Thr 
                485                 490                 495     


Met Tyr Val Thr Asp Lys Asp Asn Thr Ser Ser Lys Val His Leu Asn 
            500                 505                 510         


Asn Val Ser Asp Thr Phe Glu Val Thr Val Thr Val His Asn Lys Ser 
        515                 520                 525             


Asp Lys Pro Gln Glu Leu Tyr Tyr Gln Ala Thr Val Gln Thr Asp Lys 
    530                 535                 540                 


Val Asp Gly Lys His Phe Ala Leu Ala Pro Lys Ala Leu Tyr Glu Thr 
545                 550                 555                 560 


Ser Trp Gln Lys Ile Thr Ile Pro Ala Asn Ser Ser Lys Gln Val Thr 
                565                 570                 575     


Val Pro Ile Asp Ala Ser Arg Phe Ser Lys Asp Leu Leu Ala Gln Met 
            580                 585                 590         


Lys Asn Gly Tyr Phe Leu Glu Gly Phe Val Arg Phe Lys Gln Asp Pro 
        595                 600                 605             


Thr Lys Glu Glu Leu Met Ser Ile Pro Tyr Ile Gly Phe Arg Gly Asp 
    610                 615                 620                 


Phe Gly Asn Leu Ser Ala Leu Glu Lys Pro Ile Tyr Asp Ser Lys Asp 
625                 630                 635                 640 


Gly Ser Ser Tyr Tyr His Glu Ala Asn Ser Asp Ala Lys Asp Gln Leu 
                645                 650                 655     


Asp Gly Asp Gly Leu Gln Phe Tyr Ala Leu Lys Asn Asn Phe Thr Ala 
            660                 665                 670         


Leu Thr Thr Glu Ser Asn Pro Trp Thr Ile Ile Lys Ala Val Lys Glu 
        675                 680                 685             


Gly Val Glu Asn Ile Glu Asp Ile Glu Ser Ser Glu Ile Thr Glu Thr 
    690                 695                 700                 


Ile Phe Ala Gly Thr Phe Ala Lys Gln Asp Asp Asp Ser His Tyr Tyr 
705                 710                 715                 720 


Ile His Arg His Ala Asn Gly Lys Pro Tyr Ala Ala Ile Ser Pro Asn 
                725                 730                 735     


Gly Asp Gly Asn Arg Asp Tyr Val Gln Phe Gln Gly Thr Phe Leu Arg 
            740                 745                 750         


Asn Ala Lys Asn Leu Val Ala Glu Val Leu Asp Lys Glu Gly Asn Val 
        755                 760                 765             


Val Trp Thr Ser Glu Val Thr Glu Gln Val Val Lys Asn Tyr Asn Asn 
    770                 775                 780                 


Asp Leu Ala Ser Thr Leu Gly Ser Thr Arg Phe Glu Lys Thr Arg Trp 
785                 790                 795                 800 


Asp Gly Lys Asp Lys Asp Gly Lys Val Val Ala Asn Gly Thr Tyr Thr 
                805                 810                 815     


Tyr Arg Val Arg Tyr Thr Pro Ile Ser Ser Gly Ala Lys Glu Gln His 
            820                 825                 830         


Thr Asp Phe Asp Val Ile Val Asp Asn Thr Thr Pro Glu Val Ala Thr 
        835                 840                 845             


Ser Ala Thr Phe Ser Thr Glu Asp Arg Arg Leu Thr Leu Ala Ser Lys 
    850                 855                 860                 


Pro Lys Thr Ser Gln Pro Val Tyr Arg Glu Arg Ile Ala Tyr Thr Tyr 
865                 870                 875                 880 


Met Asp Glu Asp Leu Pro Thr Thr Glu Tyr Ile Ser Pro Asn Glu Asp 
                885                 890                 895     


Gly Thr Phe Thr Leu Pro Glu Glu Ala Glu Thr Met Glu Gly Ala Thr 
            900                 905                 910         


Val Pro Leu Lys Met Ser Asp Phe Thr Tyr Val Val Glu Asp Met Ala 
        915                 920                 925             


Gly Asn Ile Thr Tyr Thr Pro Val Thr Lys Leu Leu Glu Gly His Ser 
    930                 935                 940                 


Asn Lys 
945     


<210>  31
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO [79-571 fragment] WT w/o leader, with G overhang from 
       cleavage

<400>  31

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Lys Val 
    450                 455                 460                 


Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 
465                 470                 475                 480 


Ser Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
                485                 490                 


<210>  32
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO [79-571 fragment] W535A w/o leader, with G overhang from 
       cleavage

<400>  32

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys Val 
    450                 455                 460                 


Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 
465                 470                 475                 480 


Ser Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
                485                 490                 


<210>  33
<211>  818
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] WT w/o leader, with G overhang from 
       cleavage

<400>  33

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Lys Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Lys 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Lys Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Lys Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
                645                 650                 655     


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
            660                 665                 670         


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
        675                 680                 685             


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
    690                 695                 700                 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
705                 710                 715                 720 


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
                725                 730                 735     


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
            740                 745                 750         


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
        755                 760                 765             


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
    770                 775                 780                 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
785                 790                 795                 800 


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
                805                 810                 815     


Gln Arg 
        


<210>  34
<211>  818
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] nnAA (e.g. pAMF) mutant w/o leader, with 
       G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (256)..(256)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (355)..(355)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (626)..(626)
<223>  Xaa = nnAA (e.g pAMF)

<400>  34

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
                645                 650                 655     


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
            660                 665                 670         


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
        675                 680                 685             


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
    690                 695                 700                 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
705                 710                 715                 720 


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
                725                 730                 735     


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
            740                 745                 750         


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
        755                 760                 765             


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
    770                 775                 780                 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
785                 790                 795                 800 


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
                805                 810                 815     


Gln Arg 
        


<210>  35
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] WT w/o leader, with G overhang from cleavage

<400>  35

Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
1               5                   10                  15      


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
            20                  25                  30          


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
        35                  40                  45              


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
    50                  55                  60                  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
65                  70                  75                  80  


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
                85                  90                  95      


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
            100                 105                 110         


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
        115                 120                 125             


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
    130                 135                 140                 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
145                 150                 155                 160 


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
                165                 170                 175     


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
            180                 185                 190         


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
        195                 200                 205             


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
    210                 215                 220                 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
225                 230                 235                 240 


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
                245                 250                 255     


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
            260                 265                 270         


Phe Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
        275                 280                 285             


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
    290                 295                 300                 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
                325                 330                 335     


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
            340                 345                 350         


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
        355                 360                 365             


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
    370                 375                 380                 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
385                 390                 395                 400 


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410     


<210>  36
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DI [full length] D277A w/o leader, with G overhang from cleavage

<400>  36

Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Lys Leu 
1               5                   10                  15      


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
            20                  25                  30          


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
        35                  40                  45              


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
    50                  55                  60                  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
65                  70                  75                  80  


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
                85                  90                  95      


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
            100                 105                 110         


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
        115                 120                 125             


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
    130                 135                 140                 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
145                 150                 155                 160 


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
                165                 170                 175     


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
            180                 185                 190         


Lys Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
        195                 200                 205             


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
    210                 215                 220                 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
225                 230                 235                 240 


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
                245                 250                 255     


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
            260                 265                 270         


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
        275                 280                 285             


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
    290                 295                 300                 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Lys Gly Asp Leu Ala 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
                325                 330                 335     


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
            340                 345                 350         


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
        355                 360                 365             


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
    370                 375                 380                 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
385                 390                 395                 400 


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410     


<210>  37
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI [full length] nnAA (e.g. pAMF) mutant w/o leader, with G 
       overhang from cleavage


<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (193)..(193)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (316)..(316)
<223>  Xaa = nnAA (e.g pAMF)

<400>  37

Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu 
1               5                   10                  15      


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
            20                  25                  30          


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
        35                  40                  45              


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
    50                  55                  60                  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
65                  70                  75                  80  


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
                85                  90                  95      


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
            100                 105                 110         


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
        115                 120                 125             


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
    130                 135                 140                 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
145                 150                 155                 160 


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
                165                 170                 175     


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
            180                 185                 190         


Xaa Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
        195                 200                 205             


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
    210                 215                 220                 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
225                 230                 235                 240 


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
                245                 250                 255     


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
            260                 265                 270         


Phe Met His Leu Asp Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
        275                 280                 285             


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
    290                 295                 300                 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
                325                 330                 335     


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
            340                 345                 350         


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
        355                 360                 365             


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
    370                 375                 380                 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
385                 390                 395                 400 


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410     


<210>  38
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADI (mut5) [full length] D277A nnAA (e.g. pAMF) mutant w/o 
       leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (193)..(193)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (316)..(316)
<223>  Xaa = nnAA (e.g pAMF)

<400>  38

Gly Thr Ala Gln Thr Pro Ile His Val Tyr Ser Glu Ile Gly Xaa Leu 
1               5                   10                  15      


Lys Lys Val Leu Leu His Arg Pro Gly Lys Glu Ile Glu Asn Leu Met 
            20                  25                  30          


Pro Asp Tyr Leu Glu Arg Leu Leu Phe Asp Asp Ile Pro Phe Leu Glu 
        35                  40                  45              


Asp Ala Gln Lys Glu His Asp Ala Phe Ala Gln Ala Leu Arg Asp Glu 
    50                  55                  60                  


Gly Ile Glu Val Leu Tyr Leu Glu Thr Leu Ala Ala Glu Ser Leu Val 
65                  70                  75                  80  


Thr Pro Glu Ile Arg Glu Ala Phe Ile Asp Glu Tyr Leu Ser Glu Ala 
                85                  90                  95      


Asn Ile Arg Gly Arg Ala Thr Lys Lys Ala Ile Arg Glu Leu Leu Met 
            100                 105                 110         


Ala Ile Glu Asp Asn Gln Glu Leu Ile Glu Lys Thr Met Ala Gly Val 
        115                 120                 125             


Gln Lys Ser Glu Leu Pro Glu Ile Pro Ala Ser Glu Lys Gly Leu Thr 
    130                 135                 140                 


Asp Leu Val Glu Ser Asn Tyr Pro Phe Ala Ile Asp Pro Met Pro Asn 
145                 150                 155                 160 


Leu Tyr Phe Thr Arg Asp Pro Phe Ala Thr Ile Gly Thr Gly Val Ser 
                165                 170                 175     


Leu Asn His Met Phe Ser Glu Thr Arg Asn Arg Glu Thr Leu Tyr Gly 
            180                 185                 190         


Xaa Tyr Ile Phe Thr His His Pro Ile Tyr Gly Gly Gly Lys Val Pro 
        195                 200                 205             


Met Val Tyr Asp Arg Asn Glu Thr Thr Arg Ile Glu Gly Gly Asp Glu 
    210                 215                 220                 


Leu Val Leu Ser Lys Asp Val Leu Ala Val Gly Ile Ser Gln Arg Thr 
225                 230                 235                 240 


Asp Ala Ala Ser Ile Glu Lys Leu Leu Val Asn Ile Phe Lys Gln Asn 
                245                 250                 255     


Leu Gly Phe Lys Lys Val Leu Ala Phe Glu Phe Ala Asn Asn Arg Lys 
            260                 265                 270         


Phe Met His Leu Ala Thr Val Phe Thr Met Val Asp Tyr Asp Lys Phe 
        275                 280                 285             


Thr Ile His Pro Glu Ile Glu Gly Asp Leu Arg Val Tyr Ser Val Thr 
    290                 295                 300                 


Tyr Asp Asn Glu Glu Leu His Ile Val Glu Glu Xaa Gly Asp Leu Ala 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Asn Leu Gly Val Glu Lys Val Asp Leu Ile Arg 
                325                 330                 335     


Cys Gly Gly Asp Asn Leu Val Ala Ala Gly Arg Glu Gln Trp Asn Asp 
            340                 345                 350         


Gly Ser Asn Thr Leu Thr Ile Ala Pro Gly Val Val Val Val Tyr Asn 
        355                 360                 365             


Arg Asn Thr Ile Thr Asn Ala Ile Leu Glu Ser Lys Gly Leu Lys Leu 
    370                 375                 380                 


Ile Lys Ile His Gly Ser Glu Leu Val Arg Gly Arg Gly Gly Pro Arg 
385                 390                 395                 400 


Cys Met Ser Met Pro Phe Glu Arg Glu Asp Ile 
                405                 410     


<210>  39
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFL [full length] WT w/ leader

<400>  39

Met His His His His His His Gly Ser Gly Ser Ser Gln Ile Arg Gln 
1               5                   10                  15      


Asn Tyr Ser Thr Asp Val Glu Ala Ala Val Asn Ser Leu Val Asn Leu 
            20                  25                  30          


Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser Leu Gly Phe Tyr Phe Asp 
        35                  40                  45              


Arg Asp Asp Val Ala Leu Glu Gly Val Ser His Phe Phe Arg Glu Leu 
    50                  55                  60                  


Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg Leu Leu Lys Met Gln Asn 
65                  70                  75                  80  


Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp Ile Lys Lys Pro Ala Glu 
                85                  90                  95      


Asp Glu Trp Gly Lys Thr Pro Asp Ala Met Lys Ala Ala Met Ala Leu 
            100                 105                 110         


Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp Leu His Ala Leu Gly Ser 
        115                 120                 125             


Ala Arg Thr Asp Pro His Leu Cys Asp Phe Leu Glu Thr His Phe Leu 
    130                 135                 140                 


Asp Glu Glu Val Lys Leu Ile Lys Lys Met Gly Asp His Leu Thr Asn 
145                 150                 155                 160 


Leu His Arg Leu Gly Gly Pro Glu Ala Gly Leu Gly Glu Tyr Leu Phe 
                165                 170                 175     


Glu Arg Leu Thr Leu Lys His Asp 
            180                 


<210>  40
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  40

Ser Ser Gln Ile Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala Val 
1               5                   10                  15      


Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser 
            20                  25                  30          


Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val Ser 
        35                  40                  45              


His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg 
    50                  55                  60                  


Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp 
65                  70                  75                  80  


Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala Met 
                85                  90                  95      


Lys Ala Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp 
            100                 105                 110         


Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp Phe 
        115                 120                 125             


Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys Met 
    130                 135                 140                 


Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala Gly 
145                 150                 155                 160 


Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Lys His Asp 
                165                 170                 


<210>  41
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFL [full length] nnAA (e.g. pAMF) mutant w/ leader


<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Xaa = nnAA (e.g pAMF)

<400>  41

Met His His His His His His Gly Ser Gly Ser Ser Gln Xaa Arg Gln 
1               5                   10                  15      


Asn Tyr Ser Thr Asp Val Glu Ala Ala Val Asn Ser Leu Val Asn Leu 
            20                  25                  30          


Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser Leu Gly Phe Tyr Phe Asp 
        35                  40                  45              


Arg Asp Asp Val Ala Leu Glu Gly Val Ser His Phe Phe Arg Glu Leu 
    50                  55                  60                  


Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg Leu Leu Lys Met Gln Asn 
65                  70                  75                  80  


Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp Ile Lys Lys Pro Ala Glu 
                85                  90                  95      


Asp Glu Trp Gly Lys Thr Pro Asp Ala Met Lys Ala Ala Met Ala Leu 
            100                 105                 110         


Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp Leu His Ala Leu Gly Ser 
        115                 120                 125             


Ala Arg Thr Asp Pro His Leu Cys Asp Phe Leu Glu Thr His Phe Leu 
    130                 135                 140                 


Asp Glu Glu Val Lys Leu Ile Lys Lys Met Gly Asp His Leu Thr Asn 
145                 150                 155                 160 


Leu His Arg Leu Gly Gly Pro Glu Ala Gly Leu Gly Glu Tyr Leu Phe 
                165                 170                 175     


Glu Arg Leu Thr Leu Lys His Asp 
            180                 


<210>  42
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hFL [full length] nnAA (e.g. pAMF) mutant w/o leader


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa = nnAA (e.g pAMF)

<400>  42

Ser Ser Gln Xaa Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala Val 
1               5                   10                  15      


Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser 
            20                  25                  30          


Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val Ser 
        35                  40                  45              


His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg 
    50                  55                  60                  


Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp 
65                  70                  75                  80  


Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala Met 
                85                  90                  95      


Lys Ala Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp 
            100                 105                 110         


Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp Phe 
        115                 120                 125             


Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys Met 
    130                 135                 140                 


Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala Gly 
145                 150                 155                 160 


Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Lys His Asp 
                165                 170                 


<210>  43
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eCRM-nnAA (e.g. pAMF) mutant


<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (212)..(212)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (244)..(244)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (264)..(264)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (385)..(385)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (526)..(526)
<223>  Xaa = nnAA (e.g pAMF)

<400>  43

Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      


Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          


Xaa Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              


Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  


Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  


Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      


Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         


Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             


Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 


Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 


Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     


Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         


Asn Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             


Ile Arg Asp Xaa Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 


Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 


Glu Lys Ala Xaa Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     


His Pro Glu Leu Ser Glu Leu Xaa Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         


Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             


Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 


Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     


Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         


Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             


Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 


Xaa Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 


Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     


Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         


Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             


His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 


Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 


Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     


Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         


Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Xaa Leu Ser 
        515                 520                 525             


Leu Phe Phe Glu Ile Lys Ser 
    530                 535 


<210>  44
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC11) W535A w/o leader, with G overhang from cleavage

<400>  44

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys Val 
    450                 455                 460                 


Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 
465                 470                 475                 480 


Ser Gly Ser 
            


<210>  45
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC21) fragment W535A w/o leader, with G overhang from 
       cleavage

<400>  45

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys Val 
    450                 455                 460                 


Ile Asp Glu Arg Asp Val Lys Leu Ser 
465                 470             


<210>  46
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC31) fragment W535A w/o leader, with G overhang from 
       cleavage

<400>  46

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys Thr Gly Leu Ala Ala Glu Trp Trp Arg Lys 
    450                 455                 460             


<210>  47
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC41) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  47

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 
        435                 440                 445             


Met Ala Arg Glu Cys 
    450             


<210>  48
<211>  443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC51) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  48

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser 
        435                 440             


<210>  49
<211>  433
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC61) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  49

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 
            420                 425                 430         


Phe 
    


<210>  50
<211>  423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC71) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  50

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 
                405                 410                 415     


Ile Thr Lys Arg Arg Trp Asp 
            420             


<210>  51
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC81) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  51

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly 
                405                 410             


<210>  52
<211>  403
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC91) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage

<400>  52

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 
385                 390                 395                 400 


Glu Ile Leu 
            


<210>  53
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of W535A w/o leader, with G overhang 
       from cleavage

<400>  53

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  54
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) w/o leader, with G 
       overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  54

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  55
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 1 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<400>  55

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  56
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 2 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<400>  56

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  57
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 3 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<400>  57

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  58
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 4 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  58

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  59
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 5 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  59

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  60
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 6 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<400>  60

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  61
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 7 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<400>  61

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  62
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 8 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<400>  62

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  63
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 9 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<400>  63

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  64
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<400>  64

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  65
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<400>  65

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  66
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  66

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  67
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<400>  67

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  68
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  68

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  69
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  69

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  70
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  70

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  71
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  71

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  72
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  72

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  73
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (298)..(298)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  73

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  74
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<400>  74

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Lys Ile Asn Leu Ser His Gln 
385                 390             


<210>  75
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (74)..(74)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  75

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Xaa Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Xaa Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  76
<211>  393
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SLO(deltaC101) fragment of SLO(deltaC11) - nnAA (e.g. pAMF) 
       mutant 10 -  w/o leader, with G overhang from cleavage


<220>
<221>  MOD_RES
<222>  (35)..(35)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (112)..(112)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (195)..(195)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (246)..(246)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (280)..(280)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (298)..(298)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (330)..(330)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (387)..(387)
<223>  Xaa = nnAA (e.g pAMF)

<400>  76

Gly Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 
1               5                   10                  15      


Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 
            20                  25                  30          


Asn Asp Xaa Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 
        35                  40                  45              


Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 
    50                  55                  60                  


Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 
65                  70                  75                  80  


Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 
                85                  90                  95      


Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Xaa 
            100                 105                 110         


Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 
        115                 120                 125             


Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 
    130                 135                 140                 


Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 
145                 150                 155                 160 


Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 
                165                 170                 175     


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 
            180                 185                 190         


Asn Ser Xaa Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 
        195                 200                 205             


Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 
    210                 215                 220                 


Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 
225                 230                 235                 240 


Lys Ser Val Thr Phe Xaa Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 
                245                 250                 255     


Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 
            260                 265                 270         


Val Lys Leu Glu Thr Ser Ser Xaa Ser Asn Asp Val Glu Ala Ala Phe 
        275                 280                 285             


Ser Ala Ala Leu Lys Gly Thr Asp Val Xaa Thr Asn Gly Lys Tyr Ser 
    290                 295                 300                 


Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
305                 310                 315                 320 


Ala Ala Glu His Asn Lys Val Val Thr Xaa Asp Phe Asp Val Ile Arg 
                325                 330                 335     


Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 
            340                 345                 350         


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 
        355                 360                 365             


Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 
    370                 375                 380                 


Ser Gly Xaa Ile Asn Leu Ser His Gln 
385                 390             


<210>  77
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-714 fragment] nnAA (e.g., pAMF) mutant w/o leader


<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (256)..(256)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (355)..(355)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (626)..(626)
<223>  Xaa = nnAA (e.g pAMF)

<400>  77

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
                645                 650                 655     


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
            660                 665                 670         


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr 
        675                 680             


<210>  78
<211>  668
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-699 fragment] nnAA (e.g., pAMF) mutant w/o leader


<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (256)..(256)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (355)..(355)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (626)..(626)
<223>  Xaa = nnAA (e.g pAMF)

<400>  78

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
                645                 650                 655     


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu 
            660                 665             


<210>  79
<211>  653
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-684 fragment] nnAA (e.g., pAMF) mutant w/o leader


<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (256)..(256)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (355)..(355)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (626)..(626)
<223>  Xaa = nnAA (e.g pAMF)

<400>  79

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys 
                645                 650             


<210>  80
<211>  840
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-849 fragment] nnAA (e.g ., pAMF)mutant w/ long leader


<220>
<221>  MOD_RES
<222>  (55)..(55)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (278)..(278)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (377)..(377)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  MOD_RES
<222>  (648)..(648)
<223>  Xaa = nnAA (e.g pAMF)

<400>  80

Met His His His His His His His His His His His His Gly Ser Gly 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Gly Gln Val Lys Ala Asp Asp Arg Ala Ser 
            20                  25                  30          


Gly Glu Thr Lys Ala Ser Asn Thr His Asp Asp Ser Leu Pro Lys Pro 
        35                  40                  45              


Glu Thr Ile Gln Glu Ala Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr 
    50                  55                  60                  


Leu Ser Gln Gln Lys Ala Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr 
65                  70                  75                  80  


Lys Thr Thr Ala Glu Ile Asn His Leu Lys Glu Gln Gln Asp Asn Glu 
                85                  90                  95      


Gln Lys Ala Leu Thr Ser Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala 
            100                 105                 110         


Ser Ser Glu Glu Thr Leu Leu Ala Gln Gly Ala Glu His Gln Arg Glu 
        115                 120                 125             


Leu Thr Ala Thr Glu Thr Glu Leu His Asn Ala Gln Ala Asp Gln His 
    130                 135                 140                 


Ser Lys Glu Thr Ala Leu Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu 
145                 150                 155                 160 


Thr Thr Arg Ala Gln Asp Leu Val Glu Gln Val Lys Thr Ser Glu Gln 
                165                 170                 175     


Asn Ile Ala Lys Leu Asn Ala Met Ile Ser Asn Pro Asp Ala Ile Thr 
            180                 185                 190         


Lys Ala Ala Gln Thr Ala Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu 
        195                 200                 205             


Leu Glu Lys Ala Lys Ala Asp Leu Glu Asn Gln Lys Ala Lys Val Lys 
    210                 215                 220                 


Lys Gln Leu Thr Glu Glu Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu 
225                 230                 235                 240 


Lys Glu Ala Glu Leu Ser Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln 
                245                 250                 255     


Asp Ser Ile Val Gly Asn Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro 
            260                 265                 270         


Leu Glu Glu Leu Lys Xaa Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala 
        275                 280                 285             


Ser Tyr Asn Asn Tyr Tyr Lys Glu His Ala Asp Gln Ile Ile Ala Lys 
    290                 295                 300                 


Ala Ser Pro Gly Asn Gln Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp 
305                 310                 315                 320 


Arg Asn Arg Phe Val Asp Pro Asp Asn Leu Thr Pro Glu Val Gln Asn 
                325                 330                 335     


Glu Leu Ala Gln Phe Ala Ala His Met Ile Asn Ser Val Arg Arg Gln 
            340                 345                 350         


Leu Gly Leu Pro Pro Val Thr Val Thr Ala Gly Ser Gln Glu Phe Ala 
        355                 360                 365             


Arg Leu Leu Ser Thr Ser Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro 
    370                 375                 380                 


Ser Phe Val Tyr Gly Gln Pro Gly Val Ser Gly His Tyr Gly Val Gly 
385                 390                 395                 400 


Pro His Asp Lys Thr Ile Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu 
                405                 410                 415     


Ile Arg Asn Asp Asp Asn Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp 
            420                 425                 430         


Val His Thr Val Asn Gly Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys 
        435                 440                 445             


Tyr Met Leu Phe Thr Asp His Leu His Gly Asn Thr Tyr Gly His Ala 
    450                 455                 460                 


Ile Asn Phe Leu Arg Val Asp Lys His Asn Pro Asn Ala Pro Val Tyr 
465                 470                 475                 480 


Leu Gly Phe Ser Thr Ser Asn Val Gly Ser Leu Asn Glu His Phe Val 
                485                 490                 495     


Met Phe Pro Glu Ser Asn Ile Ala Asn His Gln Arg Phe Asn Lys Thr 
            500                 505                 510         


Pro Ile Lys Ala Val Gly Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly 
        515                 520                 525             


Thr Val Ser Asp Thr Ile Ala Ala Ile Lys Gly Lys Val Ser Ser Leu 
    530                 535                 540                 


Glu Asn Arg Leu Ser Ala Ile His Gln Glu Ala Asp Ile Met Ala Ala 
545                 550                 555                 560 


Gln Ala Lys Val Ser Gln Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys 
                565                 570                 575     


Gln Ser Asp Ser Leu Asn Leu Gln Val Arg Gln Leu Asn Asp Thr Lys 
            580                 585                 590         


Gly Ser Leu Arg Thr Glu Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln 
        595                 600                 605             


Leu Glu Ala Thr Arg Asp Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys 
    610                 615                 620                 


Ala Ala Leu His Gln Thr Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg 
625                 630                 635                 640 


Val Thr Ala Leu Val Ala Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp 
                645                 650                 655     


Phe Lys Leu Asn Pro Asn Arg Leu Gln Val Ile Arg Glu Arg Ile Asp 
            660                 665                 670         


Asn Thr Lys Gln Asp Leu Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala 
        675                 680                 685             


Gln Glu Ala Leu Ala Ala Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala 
    690                 695                 700                 


Thr Ile Ala Thr Thr Glu His Gln Leu Thr Leu Leu Lys Thr Leu Ala 
705                 710                 715                 720 


Asn Glu Lys Glu Tyr Arg His Leu Asp Glu Asp Ile Ala Thr Val Pro 
                725                 730                 735     


Asp Leu Gln Val Ala Pro Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr 
            740                 745                 750         


Ser Lys Ile Asp Thr Thr Pro Leu Val Gln Glu Met Val Lys Glu Thr 
        755                 760                 765             


Lys Gln Leu Leu Glu Ala Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser 
    770                 775                 780                 


Leu Val Ala Glu Ala Leu Val Gly Gln Thr Ser Glu Met Val Ala Ser 
785                 790                 795                 800 


Asn Ala Ile Val Ser Lys Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser 
                805                 810                 815     


Lys Thr Ser Tyr Gly Ser Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser 
            820                 825                 830         


Asp Val Asp Glu Ser Thr Gln Arg 
        835                 840 


<210>  81
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-594 fragment] nnAA (e.g., pAMF)mutant w/long leader


<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (255)..(255)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (354)..(354)
<223>  Xaa = nnAA (e.g pAMF)

<400>  81

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Xaa 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val 
        


<210>  82
<211>  625
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-657 fragment] nnAA (e.g., pAMF)mutant w/long leader


<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (255)..(255)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (354)..(354)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (625)..(625)
<223>  Xaa = nnAA (e.g pAMF)

<400>  82

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Xaa 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu Leu 
                565                 570                 575     


Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp Gln 
            580                 585                 590         


Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr Glu 
        595                 600                 605             


Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala Lys 
    610                 615                 620                 


Xaa 
625 


<210>  83
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyAD [33-667 fragment] nnAA (e.g., pAMF)mutant w/long leader


<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (255)..(255)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (354)..(354)
<223>  Xaa = nnAA (e.g pAMF)

<220>
<221>  misc_feature
<222>  (625)..(625)
<223>  Xaa = nnAA (e.g pAMF)

<400>  83

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Xaa 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu Leu 
                565                 570                 575     


Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp Gln 
            580                 585                 590         


Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr Glu 
        595                 600                 605             


Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala Lys 
    610                 615                 620                 


Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys 
625                 630                 635 


