<110>    SNU R&DB Foundation
         Shaperon

<120>    Bispecific single domain antibodies against PD-L1 and CD47 and
         use thereof

<130>    PCT2022-004

<150>    KR 10-2021-0022808
<151>    2021-02-19

<160>    22

<170>    KoPatentIn 3.0

<210>    1
<211>    120
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01


<400>    1
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser
        115                 120


<210>    2
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR1


<400>    2
Gly Phe Thr Phe Ser Ser Phe Ala
  1               5            


<210>    3
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR2


<400>    3
Ile Asn Thr Gly Gly Asp Ser Thr
  1               5            


<210>    4
<211>    13
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR3


<400>    4
Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln
  1               5                  10            


<210>    5
<211>    357
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1Nb#01-IgG1


<400>    5
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro Glu Pro Lys
        115                 120                 125 

Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
    130                 135                 140 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175 

Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
        195                 200                 205 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
225                 230                 235                 240 

Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255 

Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
            260                 265                 270 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
305                 310                 315                 320 

Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
                325                 330                 335 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350 

Leu Ser Pro Gly Lys
        355        


<210>    6
<211>    123
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01


<400>    6
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg Lys
             20                  25                  30 

Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg
         35                  40                  45 

Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
            100                 105                 110 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
        115                 120            


<210>    7
<211>    9
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR1


<400>    7
Gly Leu Thr Phe Phe Arg Lys Tyr Ala
  1               5                


<210>    8
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR2


<400>    8
Ile Tyr Ser Asp Arg Arg Ile
  1               5        


<210>    9
<211>    16
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR3


<400>    9
Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
  1               5                  10                  15 




<210>    10
<211>    357
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb-IgG4


<400>    10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg Lys
             20                  25                  30 

Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg
         35                  40                  45 

Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
            100                 105                 110 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro
        115                 120                 125 

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
    130                 135                 140 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
        195                 200                 205 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
225                 230                 235                 240 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
            260                 265                 270 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
305                 310                 315                 320 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
                325                 330                 335 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350 

Leu Ser Pro Gly Lys
        355        


<210>    11
<211>    380
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PPC Nb


<400>    11
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Gly Pro Ser
        115                 120                 125 

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
    130                 135                 140 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
145                 150                 155                 160 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
                165                 170                 175 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
            180                 185                 190 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
        195                 200                 205 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
    210                 215                 220 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
225                 230                 235                 240 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly
                245                 250                 255 

Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
            260                 265                 270 

Gly Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg
        275                 280                 285 

Lys Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu
    290                 295                 300 

Arg Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala
305                 310                 315                 320 

Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val
                325                 330                 335 

Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr
            340                 345                 350 

Cys Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp
        355                 360                 365 

Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
    370                 375                 380


<210>    12
<211>    734
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PPC Nb-IgG4


<400>    12
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gln Val Gln Leu Val Glu Ser Gly
        115                 120                 125 

Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala
    130                 135                 140 

Ser Gly Phe Thr Phe Ser Ser Phe Ala Met Ser Trp Val Arg Gln Ala
145                 150                 155                 160 

Pro Gly Lys Gly Leu Glu Trp Val Ser Asp Ile Asn Thr Gly Gly Asp
                165                 170                 175 

Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg
            180                 185                 190 

Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro
        195                 200                 205 

Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Gly Pro Lys Glu Met Val
    210                 215                 220 

His Val Tyr Ser Gln Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser
225                 230                 235                 240 

Gly Gly Ser Gly Gly Gly Pro Ser Gln Val Gln Leu Val Glu Ser Gly
                245                 250                 255 

Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala
            260                 265                 270 

Ser Gly Phe Thr Phe Ser Ser Phe Ala Met Ser Trp Val Arg Gln Ala
        275                 280                 285 

Pro Gly Lys Gly Leu Glu Trp Val Ser Asp Ile Asn Thr Gly Gly Asp
    290                 295                 300 

Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg
305                 310                 315                 320 

Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro
                325                 330                 335 

Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Gly Pro Lys Glu Met Val
            340                 345                 350 

His Val Tyr Ser Gln Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser
        355                 360                 365 

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gln Val Gln Leu Val Glu Ser
    370                 375                 380 

Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Asn Leu Ser Cys Ala
385                 390                 395                 400 

Val Ser Gly Leu Thr Phe Phe Arg Lys Tyr Ala Val Gly Trp Ala Arg
                405                 410                 415 

Gln Val Pro Gly Lys Gly Leu Glu Arg Val Ser Arg Ile Tyr Ser Asp
            420                 425                 430 

Arg Arg Ile Glu Tyr Leu Asp Ala Val Lys Gly Arg Phe Thr Ile Ser
        435                 440                 445 

Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys
    450                 455                 460 

Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Gly Leu Arg Val Gly
465                 470                 475                 480 

Leu Thr Gly Trp Asp Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val
                485                 490                 495 

Thr Val Ser Ser Gly Pro Gly Gly Pro Glu Ser Lys Tyr Gly Pro Pro
            500                 505                 510 

Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe
        515                 520                 525 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
    530                 535                 540 

Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val
545                 550                 555                 560 

Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
                565                 570                 575 

Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val
            580                 585                 590 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
        595                 600                 605 

Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser
    610                 615                 620 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
625                 630                 635                 640 

Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
                645                 650                 655 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
            660                 665                 670 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
        675                 680                 685 

Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp
    690                 695                 700 

Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
705                 710                 715                 720 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                725                 730                


<210>    13
<211>    25
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR1


<400>    13
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser
             20                  25


<210>    14
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR2


<400>    14
Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser
  1               5                  10                  15 

Asp
   


<210>    15
<211>    38
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR3


<400>    15
Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
  1               5                  10                  15 

Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp
             20                  25                  30 

Thr Ala Val Tyr Tyr Cys
         35            


<210>    16
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR4


<400>    16
Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser
  1               5                  10    


<210>    17
<211>    25
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR1


<400>    17
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser
             20                  25


<210>    18
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR2


<400>    18
Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg Val Ser
  1               5                  10                  15 

Arg
   


<210>    19
<211>    38
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR3


<400>    19
Glu Tyr Leu Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
  1               5                  10                  15 

Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp
             20                  25                  30 

Thr Ala Val Tyr Tyr Cys
         35            


<210>    20
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR4


<400>    20
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
  1               5                  10    


<210>    21
<211>    246
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PP Nb


<400>    21
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gln Val
        115                 120                 125 

Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu
    130                 135                 140 

Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Ala Met
145                 150                 155                 160 

Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Asp
                165                 170                 175 

Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly
            180                 185                 190 

Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln
        195                 200                 205 

Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys
    210                 215                 220 

Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln Gly Thr
225                 230                 235                 240 

Gln Val Thr Val Ser Ser
                245    


<210>    22
<211>    480
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PP Nb-IgG4


<400>    22
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gln Val
        115                 120                 125 

Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu
    130                 135                 140 

Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Ala Met
145                 150                 155                 160 

Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Asp
                165                 170                 175 

Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly
            180                 185                 190 

Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln
        195                 200                 205 

Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys
    210                 215                 220 

Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln Gly Thr
225                 230                 235                 240 

Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro Glu Ser Lys Tyr Gly
                245                 250                 255 

Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser
            260                 265                 270 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
        275                 280                 285 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro
    290                 295                 300 

Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
305                 310                 315                 320 

Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val
                325                 330                 335 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
            340                 345                 350 

Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr
        355                 360                 365 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
    370                 375                 380 

Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
385                 390                 395                 400 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
                405                 410                 415 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
            420                 425                 430 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser
        435                 440                 445 

Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
    450                 455                 460 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
465                 470                 475                 480
