<110>    SNU R&DB Foundation
         Shaperon

<120>    Single domain antibodies against PD-L1 and use thereof

<130>    PCT2022-003

<150>    KR 10-2021-0022807
<151>    2021-02-19

<160>    11

<170>    KoPatentIn 3.0

<210>    1
<211>    120
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01


<400>    1
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser
        115                 120


<210>    2
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR1


<400>    2
Gly Phe Thr Phe Ser Ser Phe Ala
  1               5            


<210>    3
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR2


<400>    3
Ile Asn Thr Gly Gly Asp Ser Thr
  1               5            


<210>    4
<211>    13
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_CDR3


<400>    4
Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln
  1               5                  10            


<210>    5
<211>    357
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01-IgG1


<400>    5
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro Glu Pro Lys
        115                 120                 125 

Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
    130                 135                 140 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175 

Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
        195                 200                 205 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
225                 230                 235                 240 

Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255 

Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
            260                 265                 270 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
305                 310                 315                 320 

Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
                325                 330                 335 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350 

Leu Ser Pro Gly Lys
        355        


<210>    6
<211>    25
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR1


<400>    6
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser
             20                  25


<210>    7
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR2


<400>    7
Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser
  1               5                  10                  15 

Asp
   


<210>    8
<211>    38
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR3


<400>    8
Asp Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
  1               5                  10                  15 

Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp
             20                  25                  30 

Thr Ala Val Tyr Tyr Cys
         35            


<210>    9
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PDL1 Nb#01_FR4


<400>    9
Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser
  1               5                  10    


<210>    10
<211>    246
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PP Nb


<400>    10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gln Val
        115                 120                 125 

Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu
    130                 135                 140 

Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Ala Met
145                 150                 155                 160 

Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Asp
                165                 170                 175 

Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly
            180                 185                 190 

Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln
        195                 200                 205 

Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys
    210                 215                 220 

Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln Gly Thr
225                 230                 235                 240 

Gln Val Thr Val Ser Ser
                245    


<210>    11
<211>    480
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PP Nb-IgG4


<400>    11
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe
             20                  25                  30 

Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Asp Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Lys Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln
            100                 105                 110 

Gly Thr Gln Val Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gln Val
        115                 120                 125 

Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu
    130                 135                 140 

Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe Ala Met
145                 150                 155                 160 

Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Asp
                165                 170                 175 

Ile Asn Thr Gly Gly Asp Ser Thr Asp Tyr Ala Asp Ser Val Lys Gly
            180                 185                 190 

Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln
        195                 200                 205 

Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys
    210                 215                 220 

Gly Pro Lys Glu Met Val His Val Tyr Ser Gln Arg Gly Gln Gly Thr
225                 230                 235                 240 

Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro Glu Ser Lys Tyr Gly
                245                 250                 255 

Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser
            260                 265                 270 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
        275                 280                 285 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro
    290                 295                 300 

Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
305                 310                 315                 320 

Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val
                325                 330                 335 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
            340                 345                 350 

Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr
        355                 360                 365 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
    370                 375                 380 

Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
385                 390                 395                 400 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
                405                 410                 415 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
            420                 425                 430 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser
        435                 440                 445 

Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
    450                 455                 460 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
465                 470                 475                 480
