                         SEQUENCE LISTING

<110>  Imperial College Innovations Ltd & Osel, Inc
 
<120>  Lactobacillus Crispatus Composition for use in Pregnant Subjects

<130>  RMC00214WO

<150>  GB2103170.3
<151>  2021-03-08

<150>  63/151474
<151>  2021-02-19

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1251
<212>  PRT
<213>  Lactobacillus crispatus SJ-3C

<400>  1

Met Asn Lys Lys Ser Gly His Asn Ile Lys Phe Lys Ser Ile Phe Val 
1               5                   10                  15      


Cys Thr Ser Ala Ile Met Ser Leu Trp Leu Gly Ala Asn Leu Thr Thr 
            20                  25                  30          


Thr Gln Val His Ala Ala Glu Asp Asn Ala Ala Pro Lys Ser Ser Glu 
        35                  40                  45              


Val Val Gly Gln Thr Asn Ser Ser Lys Asp Asn Ala Ala Thr Ala Thr 
    50                  55                  60                  


Val Gln Asn Gln Ser Asn Ala Lys Ala Lys Gln Arg Gln Gln Gly Val 
65                  70                  75                  80  


Ala Pro Gln Asn Val Pro Thr Val Leu Ala Ala Lys Thr Lys Asp Glu 
                85                  90                  95      


Gly Thr Asn Asn Ser Pro Ala Arg Glu Glu Val Lys Asp Gln Thr Lys 
            100                 105                 110         


Val Val Ile His Tyr Asn Gly Asp Gly Glu Lys Trp Val Pro Tyr Ile 
        115                 120                 125             


Trp Gly Lys Lys Pro Asn Gly Asn Gly Asn Glu Tyr Lys Trp Ala Gly 
    130                 135                 140                 


Lys Asp Asp Asn Gly Tyr Tyr Ser Val Ile Asp Leu Asp Lys Asn Tyr 
145                 150                 155                 160 


Asp Gln Val Gly Val Leu Ile Lys Thr Lys Gly Ser Trp Ala Gly Lys 
                165                 170                 175     


Asp Gly Thr Gly Ala Asp Arg Ser Leu Asn Val Ser Asp Asn Gly Lys 
            180                 185                 190         


Ala Glu Val Phe Tyr Lys Ala Gly Ser Asp Asp Ala Gln Lys Val Thr 
        195                 200                 205             


Pro Glu Tyr His Ser Ala Asn Ile Asn Leu His Tyr Tyr Gly Asp Asp 
    210                 215                 220                 


Gln Val Asn Lys Val Asn Val Trp Thr Asp Lys Asp Gln Asp Asn Lys 
225                 230                 235                 240 


Gln Leu Ile Ser Leu Thr Lys Gln Gly Asn Ser Gln Asp Gly Ser Ile 
                245                 250                 255     


Lys Leu Asp Asn Thr Asp Phe Ser Lys Leu Phe Val Ala Pro Val Gly 
            260                 265                 270         


Ser Asp Glu Ile Thr Arg Glu Phe Thr Pro Leu Pro Gly Asn Lys Ala 
        275                 280                 285             


Thr Asp Ile Tyr Leu Val Lys Asn Asp Thr Glu Ala Tyr Tyr Ser Lys 
    290                 295                 300                 


Ser Phe Ala Leu Ala Asp Gln Ser Leu Ser Ser Ala Tyr Met Thr Ser 
305                 310                 315                 320 


Pro Asp Ser Leu Thr Val Glu Ala Gly Lys Ala Thr Thr Val Asp Lys 
                325                 330                 335     


Ile Lys Gly Lys Leu Ser Leu Arg Asn Asn Ser Ile Asp His Ile Glu 
            340                 345                 350         


Ala Val Asp Ala Asn Ala Asp Gly Lys Ala Lys Lys Phe Ile Ile His 
        355                 360                 365             


Thr Lys Thr Asp Ile Asp Ile Leu Lys Asp Asn Gln Val Gly Leu Gly 
    370                 375                 380                 


Met Asn Phe Lys Asn Val Asp Ile Gly Pro Tyr Val Arg Ser Lys Ala 
385                 390                 395                 400 


Phe Asp Asp Lys Tyr Ala Tyr Asp Gly Asp Asp Leu Gly Cys Thr Tyr 
                405                 410                 415     


Asp Ser Lys Lys Thr Gln Ile Lys Leu Trp Ala Pro Thr Ala Lys Asp 
            420                 425                 430         


Val Glu Leu Asn Leu Tyr Asp Ser Val Asp Asn Ser Ala Lys Pro Thr 
        435                 440                 445             


Asn Val Ile Ala Leu Thr Arg Gly Asp Arg Gly Val Trp Thr Gly Ile 
    450                 455                 460                 


Ile Lys Gly Asp Lys Lys Gly Trp Ala Tyr Asp Phe Lys Leu Thr Phe 
465                 470                 475                 480 


Gly Asn Gly Lys Ile Thr Gln Thr Asp Asp Pro Tyr Ser Lys Ala Val 
                485                 490                 495     


Thr Val Asn Gly Val Arg Ser Val Ile Glu Asp Tyr Asn Asn Ile Lys 
            500                 505                 510         


Pro Thr Asp Phe Asn Arg Met Pro Ser Phe Ser Lys Pro Thr Asp Ala 
        515                 520                 525             


Ile Val Tyr Glu Ile Ser Ile Arg Asp Phe Ser Ser Asp Pro Asn Ser 
    530                 535                 540                 


Gly Ile Lys Asp Lys Gly Lys Phe Leu Gly Met Ile Glu Ser Gly Lys 
545                 550                 555                 560 


Thr Pro Thr Gly Gln Val Thr Gly Leu Asp Tyr Leu Lys Ala Leu Gly 
                565                 570                 575     


Ile Thr His Val Gln Ile Met Pro Ser Tyr Asp Phe Ala Ser Ile Asp 
            580                 585                 590         


Glu Thr Lys Ser Leu Lys Asn Gln Tyr Asn Trp Gly Tyr Asp Pro Lys 
        595                 600                 605             


Asn Tyr Asp Val Pro Glu Gly Ser Tyr Ser Ser Asp Pro Thr Asn Pro 
    610                 615                 620                 


Thr Ala Arg Ile Ile Glu Met Lys Glu Met Ile Asn Gly Leu His Lys 
625                 630                 635                 640 


Ala Gly Ile Arg Val Ile Met Asp Val Val Tyr Asn His Val Tyr Asn 
                645                 650                 655     


Pro Gln Glu Gln Ala Phe Glu Leu Thr Val Pro Gly Tyr Tyr Phe Arg 
            660                 665                 670         


Tyr Asp Ala Asp Asn Asn Thr Thr Ser Asp Ser Gly Cys Gly Asn Asp 
        675                 680                 685             


Ile Gly Ser Gln Arg Lys Met Val Arg Lys Tyr Ile Val Asp Ser Val 
    690                 695                 700                 


Val Tyr Trp Ala Lys Asn Tyr Asn Leu Asp Gly Phe Arg Phe Asp Ile 
705                 710                 715                 720 


Met Ser Leu Leu Asp Arg Glu Thr Met Asn Asp Val Arg Ala Ala Leu 
                725                 730                 735     


Asp Lys Ile Asp Pro Gly Ile Ile Thr Tyr Gly Glu Gly Trp Glu Val 
            740                 745                 750         


Gly Asn Gln Lys Tyr Thr Arg Glu Glu Gly Thr Thr Gln Tyr Thr Ala 
        755                 760                 765             


Asp Lys Val Pro Asn Val Gly Phe Phe Ser Asp Asp Met Arg Asn Ser 
    770                 775                 780                 


Val Lys Gly Asn Asn Asp Gly Ser Glu Pro Gly Leu Val Ile Gly Asn 
785                 790                 795                 800 


Gly Asn Glu Lys Asn Phe Asp Lys His Ala Val Leu Phe Thr Asp Ser 
                805                 810                 815     


Phe Leu Arg Gly Lys Trp Leu His Asn Asp Ser Trp Glu Pro His Asn 
            820                 825                 830         


Tyr Ala Thr Pro Ser Gln Met Val Asn Tyr Ala Ala Cys His Asp Gly 
        835                 840                 845             


Lys Thr Leu Tyr Asp Tyr Ile Lys Ala Ala Met Pro Asn Glu Ser Asp 
    850                 855                 860                 


Glu Asn Ile Ala Arg Arg Val Arg Leu Ala Asn Ser Met Ile Met Leu 
865                 870                 875                 880 


Ser Gln Gly Ile Ser Phe Phe His Ser Gly Gln Glu Ala Leu Arg Thr 
                885                 890                 895     


Lys Gly Gly Asn Ser Asn Ser Tyr Asn Ser Pro Val Asn Ile Asn Glu 
            900                 905                 910         


Ile Asn Trp Glu Arg Val Gly Ala Asn Lys Ala Ser Val Gln Tyr Phe 
        915                 920                 925             


Gln Lys Leu Ala Lys Leu Arg Lys Gln Leu Pro Ile Leu His Leu Asn 
    930                 935                 940                 


Asp Phe Asn Lys Ile Tyr Asp Glu Asn Val Thr Lys Ile Ile Ser Tyr 
945                 950                 955                 960 


Gly Arg Glu Thr Lys Asp Lys Asn Ile Lys Gly Val Phe Glu Tyr Glu 
                965                 970                 975     


Tyr Asn Val Asn Gly Lys Lys Leu Leu Ile Val Phe Asn Val Asn Asn 
            980                 985                 990         


Asp Lys Val Ala Leu Asn Asn Val  Asp Leu Ser His Gly  Lys Lys Leu 
        995                 1000                 1005             


Leu Asp  Ser Asp Gly Asn Val  Lys Leu Ser Asp Lys  Thr Met Leu 
    1010                 1015                 1020             


Ala Pro  Leu Ser Thr Leu Val  Val Asp Glu Asp Gly  Thr Met Val 
    1025                 1030                 1035             


Leu Pro  Asp Val Pro Lys Pro  Asp Gln Pro Val Ser  Pro Thr Thr 
    1040                 1045                 1050             


Pro Gly  Asp Lys Pro Gly Lys  Pro Thr Thr Pro Glu  Asn Pro Arg 
    1055                 1060                 1065             


Ile Pro  Asp Gln Lys Pro Ala  Asn Pro Ile Val Pro  Gln Glu Thr 
    1070                 1075                 1080             


Ile Ile  Thr Pro Glu Ala Pro  Ser Glu Asn Ser Ile  Ile Asp Asn 
    1085                 1090                 1095             


Thr Lys  Asn Ile Ile Asn Gly  Ser Glu Glu Thr Thr  Thr Val Ser 
    1100                 1105                 1110             


Thr Gln  Asp Asn Thr Thr Thr  Glu Ile Leu Leu Thr  His Asn Ala 
    1115                 1120                 1125             


Tyr Val  Tyr Asp Ser Glu Ala  Asn Pro Ile Tyr Gln  Asn Asp Asn 
    1130                 1135                 1140             


Lys Leu  Val Leu Lys Ala Gly  Asp Thr Ile Lys Pro  Leu Asp Asn 
    1145                 1150                 1155             


Gly Lys  Lys Tyr Ser Ile Lys  Gly Lys Leu Phe Tyr  Lys Val Ala 
    1160                 1165                 1170             


Lys Asn  Gln Phe Val Lys Val  Ala Asn Thr Val Ser  Tyr Tyr Lys 
    1175                 1180                 1185             


Leu Ala  His Asn Ser Phe Val  Tyr Asn Lys His Gly  Lys Ala Val 
    1190                 1195                 1200             


Lys Lys  Asn Gly Lys Arg Ile  Leu Leu Lys Lys Asn  Lys His Ile 
    1205                 1210                 1215             


Ser Leu  His Ser Asp Lys Val  Val Lys Ile Lys Gly  Lys Lys Phe 
    1220                 1225                 1230             


Tyr Gln  Leu Lys Asp Gly Ser  Phe Ile Lys Ala Arg  Asn Val Lys 
    1235                 1240                 1245             


Arg Ile  Gly 
    1250     


<210>  2
<211>  1259
<212>  PRT
<213>  Lactobacillus crispatus CTV-05

<400>  2

Met Ile Leu Trp Arg Asn Leu Phe Met Asn Lys Lys Ser Gly His Asn 
1               5                   10                  15      


Ile Lys Phe Lys Ser Ile Phe Val Cys Thr Ser Ala Ile Met Ser Leu 
            20                  25                  30          


Trp Leu Gly Ala Asn Leu Thr Thr Thr Gln Val His Ala Ala Glu Asp 
        35                  40                  45              


Asn Ala Ala Pro Lys Ser Ser Glu Val Val Gly Gln Thr Asn Ser Ser 
    50                  55                  60                  


Lys Asp Asn Ala Ala Thr Ala Thr Val Gln Asn Gln Ser Asn Ala Lys 
65                  70                  75                  80  


Ala Lys Gln Arg Gln Gln Gly Val Ala Pro Gln Asn Val Pro Thr Val 
                85                  90                  95      


Leu Ala Ala Lys Thr Lys Asp Glu Gly Thr Asn Asn Ser Pro Ala Arg 
            100                 105                 110         


Glu Glu Val Lys Asp Gln Thr Lys Val Val Ile His Tyr Asn Gly Asp 
        115                 120                 125             


Gly Glu Lys Trp Val Pro Tyr Ile Trp Gly Lys Lys Pro Asn Gly Asn 
    130                 135                 140                 


Gly Asn Glu Tyr Lys Trp Ala Gly Lys Asp Asp Asn Gly Tyr Tyr Ser 
145                 150                 155                 160 


Val Ile Asp Leu Asp Lys Asn Tyr Asp Gln Val Gly Val Leu Ile Lys 
                165                 170                 175     


Thr Lys Gly Ser Trp Ala Gly Lys Asp Gly Thr Gly Ala Asp Arg Ser 
            180                 185                 190         


Leu Asn Val Ser Asp Asn Gly Lys Ala Glu Val Phe Tyr Lys Ala Gly 
        195                 200                 205             


Ser Asp Asp Ala Gln Lys Val Thr Pro Glu Tyr His Ser Ala Asn Ile 
    210                 215                 220                 


Asn Leu His Tyr Tyr Gly Asp Asp Gln Val Asn Lys Val Asn Val Trp 
225                 230                 235                 240 


Thr Asp Lys Asp Gln Asp Asn Lys Gln Leu Ile Ser Leu Thr Lys Gln 
                245                 250                 255     


Gly Asn Ser Gln Asp Gly Ser Ile Lys Leu Asp Asn Thr Asp Phe Ser 
            260                 265                 270         


Lys Leu Phe Val Ala Pro Val Gly Ser Asp Glu Ile Thr Arg Glu Phe 
        275                 280                 285             


Thr Pro Leu Pro Gly Asn Lys Ala Thr Asp Ile Tyr Leu Val Lys Asn 
    290                 295                 300                 


Asp Thr Glu Ala Tyr Tyr Ser Lys Ser Phe Ala Leu Ala Asp Gln Ser 
305                 310                 315                 320 


Leu Ser Ser Ala Tyr Met Thr Ser Pro Asp Ser Leu Thr Val Glu Ala 
                325                 330                 335     


Gly Lys Ala Thr Thr Val Asp Lys Ile Lys Gly Lys Leu Ser Leu Arg 
            340                 345                 350         


Asn Asn Ser Ile Asp His Ile Glu Ala Val Asp Ala Asn Ala Asp Gly 
        355                 360                 365             


Lys Ala Lys Lys Phe Ile Ile His Thr Lys Thr Asp Ile Asp Ile Leu 
    370                 375                 380                 


Lys Asp Asn Gln Val Gly Leu Gly Met Asn Phe Lys Asn Val Asp Ile 
385                 390                 395                 400 


Gly Pro Tyr Val Arg Ser Lys Ala Phe Asp Asp Lys Tyr Ala Tyr Asp 
                405                 410                 415     


Gly Asp Asp Leu Gly Cys Thr Tyr Asp Ser Lys Lys Thr Gln Ile Lys 
            420                 425                 430         


Leu Trp Ala Pro Thr Ala Lys Asp Val Glu Leu Asn Leu Tyr Asp Ser 
        435                 440                 445             


Val Asp Asn Ser Ala Lys Pro Thr Asn Val Ile Ala Leu Thr Arg Gly 
    450                 455                 460                 


Asp Arg Gly Val Trp Thr Gly Ile Ile Lys Gly Asp Lys Lys Gly Trp 
465                 470                 475                 480 


Ala Tyr Asp Phe Lys Leu Thr Phe Gly Asn Gly Lys Ile Thr Gln Thr 
                485                 490                 495     


Asp Asp Pro Tyr Ser Lys Ala Val Thr Val Asn Gly Val Arg Ser Val 
            500                 505                 510         


Ile Glu Asp Tyr Asn Asn Ile Lys Pro Thr Asp Phe Asn Arg Met Pro 
        515                 520                 525             


Ser Phe Ser Lys Pro Thr Asp Ala Ile Val Tyr Glu Ile Ser Ile Arg 
    530                 535                 540                 


Asp Phe Ser Ser Asp Pro Asn Ser Gly Ile Lys Asp Lys Gly Lys Phe 
545                 550                 555                 560 


Leu Gly Met Ile Glu Ser Gly Lys Thr Pro Thr Gly Gln Val Thr Gly 
                565                 570                 575     


Leu Asp Tyr Leu Lys Ala Leu Gly Ile Thr His Val Gln Ile Met Pro 
            580                 585                 590         


Ser Tyr Asp Phe Ala Ser Ile Asp Glu Thr Lys Ser Leu Lys Asn Gln 
        595                 600                 605             


Tyr Asn Trp Gly Tyr Asp Pro Lys Asn Tyr Asp Val Pro Glu Gly Ser 
    610                 615                 620                 


Tyr Ser Ser Asp Pro Thr Asn Pro Thr Ala Arg Ile Ile Glu Met Lys 
625                 630                 635                 640 


Glu Met Ile Asn Gly Leu His Lys Ala Gly Ile Arg Val Ile Met Asp 
                645                 650                 655     


Val Val Tyr Asn His Val Tyr Asn Pro Gln Glu Gln Ala Phe Glu Leu 
            660                 665                 670         


Thr Val Pro Gly Tyr Tyr Phe Arg Tyr Asp Ala Asp Asn Asn Thr Thr 
        675                 680                 685             


Ser Asp Ser Gly Cys Gly Asn Asp Ile Gly Ser Gln Arg Lys Met Val 
    690                 695                 700                 


Arg Lys Tyr Ile Val Asp Ser Val Val Tyr Trp Ala Lys Asn Tyr Asn 
705                 710                 715                 720 


Leu Asp Gly Phe Arg Phe Asp Ile Met Ser Leu Leu Asp Arg Glu Thr 
                725                 730                 735     


Met Asn Asp Val Arg Ala Ala Leu Asp Lys Ile Asp Pro Gly Ile Ile 
            740                 745                 750         


Thr Tyr Gly Glu Gly Trp Glu Val Gly Asn Gln Lys Tyr Thr Arg Glu 
        755                 760                 765             


Glu Gly Thr Thr Gln Tyr Thr Ala Asp Lys Val Pro Asn Val Gly Phe 
    770                 775                 780                 


Phe Ser Asp Asp Met Arg Asn Ser Val Lys Gly Asn Asn Asp Gly Ser 
785                 790                 795                 800 


Glu Pro Gly Leu Val Ile Gly Asn Gly Asn Glu Lys Asn Phe Asp Lys 
                805                 810                 815     


His Ala Val Leu Phe Thr Asp Ser Phe Leu Arg Gly Lys Trp Leu His 
            820                 825                 830         


Asn Asp Ser Trp Glu Pro His Asn Tyr Ala Thr Pro Ser Gln Met Val 
        835                 840                 845             


Asn Tyr Ala Ala Cys His Asp Gly Lys Thr Leu Tyr Asp Tyr Ile Lys 
    850                 855                 860                 


Ala Ala Met Pro Asn Glu Ser Asp Glu Asn Ile Ala Arg Arg Val Arg 
865                 870                 875                 880 


Leu Ala Asn Ser Met Ile Met Leu Ser Gln Gly Ile Ser Phe Phe His 
                885                 890                 895     


Ser Gly Gln Glu Ala Leu Arg Thr Lys Gly Gly Asn Ser Asn Ser Tyr 
            900                 905                 910         


Asn Ser Pro Val Asn Ile Asn Glu Ile Asn Trp Glu Arg Val Gly Ala 
        915                 920                 925             


Asn Lys Ala Ser Val Gln Tyr Phe Gln Lys Leu Ala Lys Leu Arg Lys 
    930                 935                 940                 


Gln Leu Pro Ile Leu His Leu Asn Asp Phe Asn Lys Ile Tyr Asp Glu 
945                 950                 955                 960 


Asn Val Thr Lys Ile Ile Ser Tyr Gly Arg Glu Thr Lys Asp Lys Asn 
                965                 970                 975     


Ile Lys Gly Val Phe Glu Tyr Glu Tyr Asn Val Asn Gly Lys Lys Leu 
            980                 985                 990         


Leu Ile Val Phe Asn Val Asn Asn  Asp Lys Val Ala Leu  Asn Asn Val 
        995                 1000                 1005             


Asp Leu  Ser His Gly Lys Lys  Leu Leu Asp Ser Asp  Gly Asn Val 
    1010                 1015                 1020             


Lys Leu  Ser Asp Lys Thr Met  Leu Ala Pro Leu Ser  Thr Leu Val 
    1025                 1030                 1035             


Val Asp  Glu Asp Gly Thr Met  Val Leu Pro Asp Val  Pro Lys Pro 
    1040                 1045                 1050             


Asp Gln  Pro Val Ser Pro Thr  Thr Pro Gly Asp Lys  Pro Gly Lys 
    1055                 1060                 1065             


Pro Thr  Thr Pro Glu Asn Pro  Arg Ile Pro Asp Gln  Lys Pro Ala 
    1070                 1075                 1080             


Asn Pro  Ile Val Pro Gln Glu  Thr Ile Ile Thr Pro  Glu Ala Pro 
    1085                 1090                 1095             


Ser Glu  Asn Ser Ile Ile Asp  Asn Thr Lys Asn Ile  Ile Asn Gly 
    1100                 1105                 1110             


Ser Glu  Glu Thr Thr Thr Val  Ser Thr Gln Asp Asn  Thr Thr Thr 
    1115                 1120                 1125             


Glu Ile  Leu Leu Thr His Asn  Ala Tyr Val Tyr Asp  Ser Glu Ala 
    1130                 1135                 1140             


Asn Pro  Ile Tyr Gln Asn Asp  Asn Lys Leu Val Leu  Lys Ala Gly 
    1145                 1150                 1155             


Asp Thr  Ile Lys Pro Leu Asp  Asn Gly Lys Lys Tyr  Ser Ile Lys 
    1160                 1165                 1170             


Gly Lys  Leu Phe Tyr Lys Val  Ala Lys Asn Gln Phe  Val Lys Val 
    1175                 1180                 1185             


Ala Asn  Thr Val Ser Tyr Tyr  Lys Leu Ala His Asn  Ser Phe Val 
    1190                 1195                 1200             


Tyr Asn  Lys His Gly Lys Ala  Val Lys Lys Asn Gly  Lys Arg Ile 
    1205                 1210                 1215             


Leu Leu  Lys Lys Asn Lys His  Ile Ser Leu His Ser  Asp Lys Val 
    1220                 1225                 1230             


Val Lys  Ile Lys Gly Lys Lys  Phe Tyr Gln Leu Lys  Asp Gly Ser 
    1235                 1240                 1245             


Phe Ile  Lys Ala Arg Asn Val  Lys Arg Ile Gly 
    1250                 1255                 


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<400>  3
cggtccttat gttcgttcga                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<400>  4
cacctggagt ggttggactt                                                   20


