                         SEQUENCE LISTING

<110>  United Kingdom Research and Innovation
 
<120>  FUSION PROTEINS AND NUCLEIC ACID CONSTRUCTS

<130>  P145160WO

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  475
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Cys Ala Val His Gly 
                85                  90                  95      


Glu Arg Leu His Leu Phe Cys Glu Lys Asp Gly Lys Ala Leu Cys Trp 
            100                 105                 110         


Val Cys Ala Gln Ser Arg Lys His Arg Asp His Ala Met Val Pro Leu 
        115                 120                 125             


Glu Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu Gln Val Ala Leu Gly 
    130                 135                 140                 


Glu Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys Leu Glu Val Glu Ile 
145                 150                 155                 160 


Ala Ile Lys Arg Ala Asp Trp Lys Lys Thr Val Glu Thr Gln Lys Ser 
                165                 170                 175     


Arg Ile His Ala Glu Phe Val Gln Gln Lys Asn Phe Leu Val Glu Glu 
            180                 185                 190         


Glu Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp Glu Arg Glu Gln Leu 
        195                 200                 205             


Arg Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala Gln Gln Ser Gln Ala 
    210                 215                 220                 


Leu Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg Cys His Ser Ser Ala 
225                 230                 235                 240 


Leu Glu Leu Leu Gln Glu Val Ile Ile Val Leu Glu Arg Ser Glu Ser 
                245                 250                 255     


Trp Asn Leu Lys Asp Leu Asp Ile Thr Ser Pro Glu Leu Arg Ser Val 
            260                 265                 270         


Cys His Val Pro Gly Leu Lys Lys Met Leu Arg Thr Cys Ala Val His 
        275                 280                 285             


Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu 
    290                 295                 300                 


Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly 
305                 310                 315                 320 


Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His 
                325                 330                 335     


Phe His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu 
            340                 345                 350         


Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg Lys Gly His 
        355                 360                 365             


Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn 
    370                 375                 380                 


Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu 
385                 390                 395                 400 


Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly 
                405                 410                 415     


Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser 
            420                 425                 430         


Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro 
        435                 440                 445             


Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro 
    450                 455                 460                 


Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
465                 470                 475 


<210>  2
<211>  85
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu 
                85  


<210>  3
<211>  180
<212>  PRT
<213>  Homo sapiens

<400>  3

Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile 
1               5                   10                  15      


Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser 
            20                  25                  30          


Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly 
        35                  40                  45              


Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr 
    50                  55                  60                  


Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg 
65                  70                  75                  80  


Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp 
                85                  90                  95      


Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro 
            100                 105                 110         


Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr 
        115                 120                 125             


Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu 
    130                 135                 140                 


Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe 
145                 150                 155                 160 


Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr 
                165                 170                 175     


Leu Cys Pro Leu 
            180 


<210>  4
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  4

Arg Cys Ala Val His Gly Glu Arg Leu His Leu Phe Cys Glu Lys Asp 
1               5                   10                  15      


Gly Lys Ala Leu Cys Trp Val Cys Ala Gln Ser Arg Lys His Arg Asp 
            20                  25                  30          


His Ala Met Val Pro Leu 
        35              


<210>  5
<211>  110
<212>  PRT
<213>  Homo sapiens

<400>  5

Glu Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu Gln Val Ala Leu Gly 
1               5                   10                  15      


Glu Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys Leu Glu Val Glu Ile 
            20                  25                  30          


Ala Ile Lys Arg Ala Asp Trp Lys Lys Thr Val Glu Thr Gln Lys Ser 
        35                  40                  45              


Arg Ile His Ala Glu Phe Val Gln Gln Lys Asn Phe Leu Val Glu Glu 
    50                  55                  60                  


Glu Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp Glu Arg Glu Gln Leu 
65                  70                  75                  80  


Arg Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala Gln Gln Ser Gln Ala 
                85                  90                  95      


Leu Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg Cys His Ser 
            100                 105                 110 


<210>  6
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-WT

<400>  6

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Asn Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  7
<211>  150
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ube2V2-WT

<400>  7

Gly Ser Gln Glu Phe Met Ala Val Ser Thr Gly Val Lys Val Pro Arg 
1               5                   10                  15      


Asn Phe Arg Leu Leu Glu Glu Leu Glu Glu Gly Gln Lys Gly Val Gly 
            20                  25                  30          


Asp Gly Thr Val Ser Trp Gly Leu Glu Asp Asp Glu Asp Met Thr Leu 
        35                  40                  45              


Thr Arg Trp Thr Gly Met Ile Ile Gly Pro Pro Arg Thr Asn Tyr Glu 
    50                  55                  60                  


Asn Arg Ile Tyr Ser Leu Lys Val Glu Cys Gly Pro Lys Tyr Pro Glu 
65                  70                  75                  80  


Ala Pro Pro Ser Val Arg Phe Val Thr Lys Ile Asn Met Asn Gly Ile 
                85                  90                  95      


Asn Asn Ser Ser Gly Met Val Asp Ala Arg Ser Ile Pro Val Leu Ala 
            100                 105                 110         


Lys Trp Gln Asn Ser Tyr Ser Ile Lys Val Val Leu Gln Glu Leu Arg 
        115                 120                 125             


Arg Leu Met Met Ser Lys Glu Asn Met Lys Leu Pro Gln Pro Pro Glu 
    130                 135                 140                 


Gly Gln Thr Tyr Asn Asn 
145                 150 


<210>  8
<211>  76
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pET17b-Ubiquitin

<400>  8

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  9
<211>  1068
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pET21d-Ube1

<400>  9

Met Gly His His His His His His Gly Gly Met Ser Ser Ser Pro Leu 
1               5                   10                  15      


Ser Lys Lys Arg Arg Val Ser Gly Pro Asp Pro Lys Pro Gly Ser Asn 
            20                  25                  30          


Cys Ser Pro Ala Gln Ser Val Leu Ser Glu Val Pro Ser Val Pro Thr 
        35                  40                  45              


Asn Gly Met Ala Lys Asn Gly Ser Glu Ala Asp Ile Asp Glu Gly Leu 
    50                  55                  60                  


Tyr Ser Arg Gln Leu Tyr Val Leu Gly His Glu Ala Met Lys Arg Leu 
65                  70                  75                  80  


Gln Thr Ser Ser Val Leu Val Ser Gly Leu Arg Gly Leu Gly Val Glu 
                85                  90                  95      


Ile Ala Lys Asn Ile Ile Leu Gly Gly Val Lys Ala Val Thr Leu His 
            100                 105                 110         


Asp Gln Gly Thr Ala Gln Trp Ala Asp Leu Ser Ser Gln Phe Tyr Leu 
        115                 120                 125             


Arg Glu Glu Asp Ile Gly Lys Asn Arg Ala Glu Val Ser Gln Pro Arg 
    130                 135                 140                 


Leu Ala Glu Leu Asn Ser Tyr Val Pro Val Thr Ala Tyr Thr Gly Pro 
145                 150                 155                 160 


Leu Val Glu Asp Phe Leu Ser Gly Phe Gln Val Val Val Leu Thr Asn 
                165                 170                 175     


Thr Pro Leu Glu Asp Gln Leu Arg Val Gly Glu Phe Cys His Asn Arg 
            180                 185                 190         


Gly Ile Lys Leu Val Val Ala Asp Thr Arg Gly Leu Phe Gly Gln Leu 
        195                 200                 205             


Phe Cys Asp Phe Gly Glu Glu Met Ile Leu Thr Asp Ser Asn Gly Glu 
    210                 215                 220                 


Gln Pro Leu Ser Ala Met Val Ser Met Val Thr Lys Asp Asn Pro Gly 
225                 230                 235                 240 


Val Val Thr Cys Leu Asp Glu Ala Arg His Gly Phe Glu Ser Gly Asp 
                245                 250                 255     


Phe Val Ser Phe Ser Glu Val Gln Gly Met Val Glu Leu Asn Gly Asn 
            260                 265                 270         


Gln Pro Met Glu Ile Lys Val Leu Gly Pro Tyr Thr Phe Ser Ile Cys 
        275                 280                 285             


Asp Thr Ser Asn Phe Ser Asp Tyr Ile Arg Gly Gly Ile Val Ser Gln 
    290                 295                 300                 


Val Lys Val Pro Lys Lys Ile Ser Phe Lys Ser Leu Val Ala Ser Leu 
305                 310                 315                 320 


Ala Glu Pro Asp Phe Val Val Thr Asp Phe Ala Lys Phe Ser Arg Pro 
                325                 330                 335     


Ala Gln Leu His Ile Gly Phe Gln Ala Leu His Gln Phe Cys Ala Gln 
            340                 345                 350         


His Gly Arg Pro Pro Arg Pro Arg Asn Glu Glu Asp Ala Ala Glu Leu 
        355                 360                 365             


Val Ala Leu Ala Gln Ala Val Asn Ala Arg Ala Leu Pro Ala Val Gln 
    370                 375                 380                 


Gln Asn Asn Leu Asp Glu Asp Leu Ile Arg Lys Leu Ala Tyr Val Ala 
385                 390                 395                 400 


Ala Gly Asp Leu Ala Pro Ile Asn Ala Phe Ile Gly Gly Leu Ala Ala 
                405                 410                 415     


Gln Glu Val Met Lys Ala Cys Ser Gly Lys Phe Met Pro Ile Met Gln 
            420                 425                 430         


Trp Leu Tyr Phe Asp Ala Leu Glu Cys Leu Pro Glu Asp Lys Glu Val 
        435                 440                 445             


Leu Thr Glu Asp Lys Cys Leu Gln Arg Gln Asn Arg Tyr Asp Gly Gln 
    450                 455                 460                 


Val Ala Val Phe Gly Ser Asp Leu Gln Glu Lys Leu Gly Lys Gln Lys 
465                 470                 475                 480 


Tyr Phe Leu Val Gly Ala Gly Ala Ile Gly Cys Glu Leu Leu Lys Asn 
                485                 490                 495     


Phe Ala Met Ile Gly Leu Gly Cys Gly Glu Gly Gly Glu Ile Ile Val 
            500                 505                 510         


Thr Asp Met Asp Thr Ile Glu Lys Ser Asn Leu Asn Arg Gln Phe Leu 
        515                 520                 525             


Phe Arg Pro Trp Asp Val Thr Lys Leu Lys Ser Asp Thr Ala Ala Ala 
    530                 535                 540                 


Ala Val Arg Gln Met Asn Pro His Ile Arg Val Thr Ser His Gln Asn 
545                 550                 555                 560 


Arg Val Gly Pro Asp Thr Glu Arg Ile Tyr Asp Asp Asp Phe Phe Gln 
                565                 570                 575     


Asn Leu Asp Gly Val Ala Asn Ala Leu Asp Asn Val Asp Ala Arg Met 
            580                 585                 590         


Tyr Met Asp Arg Arg Cys Val Tyr Tyr Arg Lys Pro Leu Leu Glu Ser 
        595                 600                 605             


Gly Thr Leu Gly Thr Lys Gly Asn Val Gln Val Val Ile Pro Phe Leu 
    610                 615                 620                 


Thr Glu Ser Tyr Ser Ser Ser Gln Asp Pro Pro Glu Lys Ser Ile Pro 
625                 630                 635                 640 


Ile Cys Thr Leu Lys Asn Phe Pro Asn Ala Ile Glu His Thr Leu Gln 
                645                 650                 655     


Trp Ala Arg Asp Glu Phe Glu Gly Leu Phe Lys Gln Pro Ala Glu Asn 
            660                 665                 670         


Val Asn Gln Tyr Leu Thr Asp Pro Lys Phe Val Glu Arg Thr Leu Arg 
        675                 680                 685             


Leu Ala Gly Thr Gln Pro Leu Glu Val Leu Glu Ala Val Gln Arg Ser 
    690                 695                 700                 


Leu Val Leu Gln Arg Pro Gln Thr Trp Ala Asp Cys Val Thr Trp Ala 
705                 710                 715                 720 


Cys His His Trp His Thr Gln Tyr Ser Asn Asn Ile Arg Gln Leu Leu 
                725                 730                 735     


His Asn Phe Pro Pro Asp Gln Leu Thr Ser Ser Gly Ala Pro Phe Trp 
            740                 745                 750         


Ser Gly Pro Lys Arg Cys Pro His Pro Leu Thr Phe Asp Val Asn Asn 
        755                 760                 765             


Pro Leu His Leu Asp Tyr Val Met Ala Ala Ala Asn Leu Phe Ala Gln 
    770                 775                 780                 


Thr Tyr Gly Leu Thr Gly Ser Gln Asp Arg Ala Ala Val Ala Thr Phe 
785                 790                 795                 800 


Leu Gln Ser Val Gln Val Pro Glu Phe Thr Pro Lys Ser Gly Val Lys 
                805                 810                 815     


Ile His Val Ser Asp Gln Glu Leu Gln Ser Ala Asn Ala Ser Val Asp 
            820                 825                 830         


Asp Ser Arg Leu Glu Glu Leu Lys Ala Thr Leu Pro Ser Pro Asp Lys 
        835                 840                 845             


Leu Pro Gly Phe Lys Met Tyr Pro Ile Asp Phe Glu Lys Asp Asp Asp 
    850                 855                 860                 


Ser Asn Phe His Met Asp Phe Ile Val Ala Ala Ser Asn Leu Arg Ala 
865                 870                 875                 880 


Glu Asn Tyr Asp Ile Pro Ser Ala Asp Arg His Lys Ser Lys Leu Ile 
                885                 890                 895     


Ala Gly Lys Ile Ile Pro Ala Ile Ala Thr Thr Thr Ala Ala Val Val 
            900                 905                 910         


Gly Leu Val Cys Leu Glu Leu Tyr Lys Val Val Gln Gly His Arg Gln 
        915                 920                 925             


Leu Asp Ser Tyr Lys Asn Gly Phe Leu Asn Leu Ala Leu Pro Phe Phe 
    930                 935                 940                 


Gly Phe Ser Glu Pro Leu Ala Ala Pro Arg His Gln Tyr Tyr Asn Gln 
945                 950                 955                 960 


Glu Trp Thr Leu Trp Asp Arg Phe Glu Val Gln Gly Leu Gln Pro Asn 
                965                 970                 975     


Gly Glu Glu Met Thr Leu Lys Gln Phe Leu Asp Tyr Phe Lys Thr Glu 
            980                 985                 990         


His Lys Leu Glu Ile Thr Met Leu  Ser Gln Gly Val Ser  Met Leu Tyr 
        995                 1000                 1005             


Ser Phe  Phe Met Pro Ala Ala  Lys Leu Lys Glu Arg  Leu Asp Gln 
    1010                 1015                 1020             


Pro Met  Thr Glu Ile Val Ser  Arg Val Ser Lys Arg  Lys Leu Gly 
    1025                 1030                 1035             


Arg His  Val Arg Ala Leu Val  Leu Glu Leu Cys Cys  Asn Asp Glu 
    1040                 1045                 1050             


Ser Gly  Glu Asp Val Glu Val  Pro Tyr Val Arg Tyr  Thr Ile Arg 
    1055                 1060                 1065             


<210>  10
<211>  88
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-TRIM21-RING

<400>  10

Gly Ser His Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu 
1               5                   10                  15      


Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile 
            20                  25                  30          


Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys 
        35                  40                  45              


Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys 
    50                  55                  60                  


Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys 
65                  70                  75                  80  


Glu Ile Ser Gln Glu Ala Arg Glu 
                85              


<210>  11
<211>  164
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-G75/76A-TRIM21-RING

<400>  11

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu 
                


<210>  12
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-C87K-K92A

<400>  12

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Lys Leu Asp Ile Leu Ala Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Asn Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  13
<211>  74
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pET17b-Ubiquitin-1-74

<400>  13

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg 
65                  70                  


<210>  14
<211>  88
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pET17b-His-Ubiquitin

<400>  14

Met Gly Ser Ser His His His His His His Ser Gly Met Gln Ile Phe 
1               5                   10                  15      


Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
            20                  25                  30          


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
        35                  40                  45              


Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp 
    50                  55                  60                  


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His 
65                  70                  75                  80  


Leu Val Leu Arg Leu Arg Gly Gly 
                85              


<210>  15
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-D119A

<400>  15

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Ala Pro Leu Ala Asn Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  16
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-D119N

<400>  16

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asn Pro Leu Ala Asn Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  17
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-N79A

<400>  17

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Ala 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Asn Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  18
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-N123A

<400>  18

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Ala Asp Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  19
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TS-Ube2N-D124A

<400>  19

Gly Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu 
1               5                   10                  15      


Leu Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn 
            20                  25                  30          


Ala Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe 
        35                  40                  45              


Glu Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro 
    50                  55                  60                  


Met Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn 
65                  70                  75                  80  


Val Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp 
                85                  90                  95      


Ser Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu 
            100                 105                 110         


Leu Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Asn Ala Val Ala Glu 
        115                 120                 125             


Gln Trp Lys Thr Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp 
    130                 135                 140                 


Thr Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150             


<210>  20
<211>  167
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pET28a-Ube2D1-WT

<400>  20

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Leu Lys Arg Ile Gln Lys Glu Leu Ser Asp 
            20                  25                  30          


Leu Gln Arg Asp Pro Pro Ala His Cys Ser Ala Gly Pro Val Gly Asp 
        35                  40                  45              


Asp Leu Phe His Trp Gln Ala Thr Ile Met Gly Pro Pro Asp Ser Ala 
    50                  55                  60                  


Tyr Gln Gly Gly Val Phe Phe Leu Thr Val His Phe Pro Thr Asp Tyr 
65                  70                  75                  80  


Pro Phe Lys Pro Pro Lys Ile Ala Phe Thr Thr Lys Ile Tyr His Pro 
                85                  90                  95      


Asn Ile Asn Ser Asn Gly Ser Ile Cys Leu Asp Ile Leu Arg Ser Gln 
            100                 105                 110         


Trp Ser Pro Ala Leu Thr Val Ser Lys Val Leu Leu Ser Ile Cys Ser 
        115                 120                 125             


Leu Leu Cys Asp Pro Asn Pro Asp Asp Pro Leu Val Pro Asp Ile Ala 
    130                 135                 140                 


Gln Ile Tyr Lys Ser Asp Lys Glu Lys Tyr Asn Arg His Ala Arg Glu 
145                 150                 155                 160 


Trp Thr Gln Lys Tyr Ala Met 
                165         


<210>  21
<211>  252
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-TRIM21-RING-RING

<400>  21

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Gly Ser His Met Ala Ser Ala Ala Arg Leu Thr Met 
                165                 170                 175     


Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu 
            180                 185                 190         


Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser 
        195                 200                 205             


Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg 
    210                 215                 220                 


Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val 
225                 230                 235                 240 


Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu 
                245                 250         


<210>  22
<211>  261
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-TRIM21-RING-RING-FLAG

<400>  22

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Gly Ser His Met Ala Ser Ala Ala Arg Leu Thr Met 
                165                 170                 175     


Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu 
            180                 185                 190         


Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser 
        195                 200                 205             


Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg 
    210                 215                 220                 


Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val 
225                 230                 235                 240 


Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Asp Tyr Lys 
                245                 250                 255     


Asp Asp Asp Asp Lys 
            260     


<210>  23
<211>  261
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-TRIM21-RING-E12R-RING-E12R-FLAG

<400>  23

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Arg Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Gly Ser His Met Ala Ser Ala Ala Arg Leu Thr Met 
                165                 170                 175     


Met Trp Arg Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu 
            180                 185                 190         


Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser 
        195                 200                 205             


Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg 
    210                 215                 220                 


Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val 
225                 230                 235                 240 


Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Asp Tyr Lys 
                245                 250                 255     


Asp Asp Asp Asp Lys 
            260     


<210>  24
<211>  497
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-mEGFP-Fc

<400>  24

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Lys Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Tyr 
225                 230                 235                 240 


Ser Asp Leu Glu Leu Lys Leu Arg Ile Leu Gln Ser Thr Ala Arg Asp 
                245                 250                 255     


Pro Val Gln Val Lys Arg Pro Leu Leu Gln Met Val Arg Ser Asp Lys 
            260                 265                 270         


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
        275                 280                 285             


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
    290                 295                 300                 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
305                 310                 315                 320 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
                325                 330                 335     


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
            340                 345                 350         


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
        355                 360                 365             


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
    370                 375                 380                 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
385                 390                 395                 400 


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
                405                 410                 415     


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
            420                 425                 430         


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
    450                 455                 460                 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
465                 470                 475                 480 


Ala Leu Ala Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                485                 490                 495     


Lys 
    


<210>  25
<211>  475
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-TRIM21

<400>  25

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Cys Ala Val His Gly 
                85                  90                  95      


Glu Arg Leu His Leu Phe Cys Glu Lys Asp Gly Lys Ala Leu Cys Trp 
            100                 105                 110         


Val Cys Ala Gln Ser Arg Lys His Arg Asp His Ala Met Val Pro Leu 
        115                 120                 125             


Glu Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu Gln Val Ala Leu Gly 
    130                 135                 140                 


Glu Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys Leu Glu Val Glu Ile 
145                 150                 155                 160 


Ala Ile Lys Arg Ala Asp Trp Lys Lys Thr Val Glu Thr Gln Lys Ser 
                165                 170                 175     


Arg Ile His Ala Glu Phe Val Gln Gln Lys Asn Phe Leu Val Glu Glu 
            180                 185                 190         


Glu Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp Glu Arg Glu Gln Leu 
        195                 200                 205             


Arg Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala Gln Gln Ser Gln Ala 
    210                 215                 220                 


Leu Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg Cys His Ser Ser Ala 
225                 230                 235                 240 


Leu Glu Leu Leu Gln Glu Val Ile Ile Val Leu Glu Arg Ser Glu Ser 
                245                 250                 255     


Trp Asn Leu Lys Asp Leu Asp Ile Thr Ser Pro Glu Leu Arg Ser Val 
            260                 265                 270         


Cys His Val Pro Gly Leu Lys Lys Met Leu Arg Thr Cys Ala Val His 
        275                 280                 285             


Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu 
    290                 295                 300                 


Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly 
305                 310                 315                 320 


Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His 
                325                 330                 335     


Phe His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu 
            340                 345                 350         


Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg Lys Gly His 
        355                 360                 365             


Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn 
    370                 375                 380                 


Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu 
385                 390                 395                 400 


Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly 
                405                 410                 415     


Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser 
            420                 425                 430         


Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro 
        435                 440                 445             


Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro 
    450                 455                 460                 


Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
465                 470                 475 


<210>  26
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-TRIM21-RING-PRYSPRY

<400>  26

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Gly Leu Lys Lys Met 
                85                  90                  95      


Leu Arg Thr Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn 
            100                 105                 110         


Pro Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp 
        115                 120                 125             


Thr Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro 
    130                 135                 140                 


Met Val Leu Gly Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu 
145                 150                 155                 160 


Val Asp Val Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp 
                165                 170                 175     


Ser Val Arg Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe 
            180                 185                 190         


Trp Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr 
        195                 200                 205             


Pro Gln Thr Pro Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile 
    210                 215                 220                 


Phe Leu Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp 
225                 230                 235                 240 


His Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro 
                245                 250                 255     


Leu Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr 
            260                 265                 270         


Ala Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr 
        275                 280                 285             


Asp Tyr 
    290 


<210>  27
<211>  375
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-TRIM21-RING-RING-PRYSPRY

<400>  27

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp 
                85                  90                  95      


Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val 
            100                 105                 110         


Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val 
        115                 120                 125             


Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu 
    130                 135                 140                 


Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn 
145                 150                 155                 160 


Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg 
                165                 170                 175     


Gly Leu Lys Lys Met Leu Arg Thr Cys Ala Val His Ile Thr Leu Asp 
            180                 185                 190         


Pro Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln 
        195                 200                 205             


Val Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg 
    210                 215                 220                 


Phe Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His Phe His Ser Gly 
225                 230                 235                 240 


Lys His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu Ala Trp Asp Leu 
                245                 250                 255     


Gly Val Cys Arg Asp Ser Val Arg Arg Lys Gly His Phe Leu Leu Ser 
            260                 265                 270         


Ser Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr 
        275                 280                 285             


Glu Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu Gln Val Pro Pro 
    290                 295                 300                 


Cys Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly Met Val Ser Phe 
305                 310                 315                 320 


Tyr Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys 
                325                 330                 335     


Ala Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp 
            340                 345                 350         


Gly Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly 
        355                 360                 365             


Ser Gln Gly Ser Thr Asp Tyr 
    370                 375 


<210>  28
<211>  560
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-TRIM21-RING-TRIM21

<400>  28

Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
1               5                   10                  15      


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            20                  25                  30          


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        35                  40                  45              


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    50                  55                  60                  


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
65                  70                  75                  80  


Gln Glu Ala Arg Glu Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp 
                85                  90                  95      


Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val 
            100                 105                 110         


Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val 
        115                 120                 125             


Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu 
    130                 135                 140                 


Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn 
145                 150                 155                 160 


Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg 
                165                 170                 175     


Cys Ala Val His Gly Glu Arg Leu His Leu Phe Cys Glu Lys Asp Gly 
            180                 185                 190         


Lys Ala Leu Cys Trp Val Cys Ala Gln Ser Arg Lys His Arg Asp His 
        195                 200                 205             


Ala Met Val Pro Leu Glu Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu 
    210                 215                 220                 


Gln Val Ala Leu Gly Glu Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys 
225                 230                 235                 240 


Leu Glu Val Glu Ile Ala Ile Lys Arg Ala Asp Trp Lys Lys Thr Val 
                245                 250                 255     


Glu Thr Gln Lys Ser Arg Ile His Ala Glu Phe Val Gln Gln Lys Asn 
            260                 265                 270         


Phe Leu Val Glu Glu Glu Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp 
        275                 280                 285             


Glu Arg Glu Gln Leu Arg Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala 
    290                 295                 300                 


Gln Gln Ser Gln Ala Leu Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg 
305                 310                 315                 320 


Cys His Ser Ser Ala Leu Glu Leu Leu Gln Glu Val Ile Ile Val Leu 
                325                 330                 335     


Glu Arg Ser Glu Ser Trp Asn Leu Lys Asp Leu Asp Ile Thr Ser Pro 
            340                 345                 350         


Glu Leu Arg Ser Val Cys His Val Pro Gly Leu Lys Lys Met Leu Arg 
        355                 360                 365             


Thr Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp 
    370                 375                 380                 


Leu Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln 
385                 390                 395                 400 


Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val 
                405                 410                 415     


Leu Gly Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu Val Asp 
            420                 425                 430         


Val Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val 
        435                 440                 445             


Arg Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr 
    450                 455                 460                 


Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln 
465                 470                 475                 480 


Thr Pro Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu 
                485                 490                 495     


Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly 
            500                 505                 510         


Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg 
        515                 520                 525             


Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro 
    530                 535                 540                 


Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
545                 550                 555                 560 


<210>  29
<211>  596
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-mCherry-TRIM21-CC-PRYSPRY

<400>  29

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Ser Gly Leu Arg 
225                 230                 235                 240 


Ser Arg Ala Gln Ala Ser Asn Ser Leu Glu Glu Ala Ala Gln Glu Tyr 
                245                 250                 255     


Gln Glu Lys Leu Gln Val Ala Leu Gly Glu Leu Arg Arg Lys Gln Glu 
            260                 265                 270         


Leu Ala Glu Lys Leu Glu Val Glu Ile Ala Ile Lys Arg Ala Asp Trp 
        275                 280                 285             


Lys Lys Thr Val Glu Thr Gln Lys Ser Arg Ile His Ala Glu Phe Val 
    290                 295                 300                 


Gln Gln Lys Asn Phe Leu Val Glu Glu Glu Gln Arg Gln Leu Gln Glu 
305                 310                 315                 320 


Leu Glu Lys Asp Glu Arg Glu Gln Leu Arg Ile Leu Gly Glu Lys Glu 
                325                 330                 335     


Ala Lys Leu Ala Gln Gln Ser Gln Ala Leu Gln Glu Leu Ile Ser Glu 
            340                 345                 350         


Leu Asp Arg Arg Cys His Ser Ser Ala Leu Glu Leu Leu Gln Glu Val 
        355                 360                 365             


Ile Ile Val Leu Glu Arg Ser Glu Ser Trp Asn Leu Lys Asp Leu Asp 
    370                 375                 380                 


Ile Thr Ser Pro Glu Leu Arg Ser Val Cys His Val Pro Gly Leu Lys 
385                 390                 395                 400 


Lys Met Leu Arg Thr Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr 
                405                 410                 415     


Ala Asn Pro Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu 
            420                 425                 430         


Gly Asp Thr Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser 
        435                 440                 445             


Tyr Pro Met Val Leu Gly Ala Gln His Phe His Ser Gly Lys His Tyr 
    450                 455                 460                 


Trp Glu Val Asp Val Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys 
465                 470                 475                 480 


Arg Asp Ser Val Arg Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser 
                485                 490                 495     


Gly Phe Trp Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly 
            500                 505                 510         


Thr Tyr Pro Gln Thr Pro Leu His Leu Gln Val Pro Pro Cys Gln Val 
        515                 520                 525             


Gly Ile Phe Leu Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile 
    530                 535                 540                 


Thr Asp His Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr 
545                 550                 555                 560 


Gly Pro Leu Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys 
                565                 570                 575     


Asn Thr Ala Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly 
            580                 585                 590         


Ser Thr Asp Tyr 
        595     


<210>  30
<211>  350
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-VhhGFP-Fc

<400>  30

Met Asp Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Val Asn 
            20                  25                  30          


Arg Tyr Ser Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Trp Val Ala Gly Met Ser Ser Ala Gly Asp Arg Ser Ser Tyr Glu Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Arg Asn Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Asn Val Asn Val Gly Phe Glu Tyr Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser Leu Gln Met Val Arg Ser Asp Lys Thr His Thr 
        115                 120                 125             


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    130                 135                 140                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
                165                 170                 175     


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        195                 200                 205             


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
    210                 215                 220                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
225                 230                 235                 240 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                245                 250                 255     


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            260                 265                 270         


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
        275                 280                 285             


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    290                 295                 300                 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
305                 310                 315                 320 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
                325                 330                 335     


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 345                 350 


<210>  31
<211>  350
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pGEMHE-VhhGFP-Fc-H433A

<400>  31

Met Asp Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Val Asn 
            20                  25                  30          


Arg Tyr Ser Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Trp Val Ala Gly Met Ser Ser Ala Gly Asp Arg Ser Ser Tyr Glu Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Arg Asn Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Asn Val Asn Val Gly Phe Glu Tyr Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser Leu Gln Met Val Arg Ser Asp Lys Thr His Thr 
        115                 120                 125             


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    130                 135                 140                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
                165                 170                 175     


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        195                 200                 205             


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
    210                 215                 220                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
225                 230                 235                 240 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                245                 250                 255     


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            260                 265                 270         


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
        275                 280                 285             


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    290                 295                 300                 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
305                 310                 315                 320 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu Ala 
                325                 330                 335     


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 345                 350 


<210>  32
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-TRIM21-RING-RING-PRYSPRY

<400>  32

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu 
                165                 170                 175     


Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser 
            180                 185                 190         


Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly 
        195                 200                 205             


Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu 
    210                 215                 220                 


Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu 
225                 230                 235                 240 


Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Gly 
                245                 250                 255     


Leu Lys Lys Met Leu Arg Thr Cys Ala Val His Ile Thr Leu Asp Pro 
            260                 265                 270         


Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln Val 
        275                 280                 285             


Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe 
    290                 295                 300                 


Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His Phe His Ser Gly Lys 
305                 310                 315                 320 


His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu Ala Trp Asp Leu Gly 
                325                 330                 335     


Val Cys Arg Asp Ser Val Arg Arg Lys Gly His Phe Leu Leu Ser Ser 
            340                 345                 350         


Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu 
        355                 360                 365             


Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu Gln Val Pro Pro Cys 
    370                 375                 380                 


Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr 
385                 390                 395                 400 


Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala 
                405                 410                 415     


Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly 
            420                 425                 430         


Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser 
        435                 440                 445             


Gln Gly Ser Thr Asp Tyr 
    450                 


<210>  33
<211>  369
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin-TRIM21-RING-PRYSPRY

<400>  33

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Gly Leu Lys Lys Met Leu 
                165                 170                 175     


Arg Thr Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro 
            180                 185                 190         


Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp Thr 
        195                 200                 205             


Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met 
    210                 215                 220                 


Val Leu Gly Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu Val 
225                 230                 235                 240 


Asp Val Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp Ser 
                245                 250                 255     


Val Arg Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp 
            260                 265                 270         


Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro 
        275                 280                 285             


Gln Thr Pro Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile Phe 
    290                 295                 300                 


Leu Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp His 
305                 310                 315                 320 


Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu 
                325                 330                 335     


Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala 
            340                 345                 350         


Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp 
        355                 360                 365             


Tyr 
    


<210>  34
<211>  682
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin4-TRIM21-RING-RING-PRYSPRY

<400>  34

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met Gln Ile Phe Val 
145                 150                 155                 160 


Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp 
                165                 170                 175     


Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro 
            180                 185                 190         


Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly 
        195                 200                 205             


Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu 
    210                 215                 220                 


Val Leu Arg Leu Arg Gly Gly Met Gln Ile Phe Val Lys Thr Leu Thr 
225                 230                 235                 240 


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
                245                 250                 255     


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
            260                 265                 270         


Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
        275                 280                 285             


Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu 
    290                 295                 300                 


Arg Ala Ala Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu 
305                 310                 315                 320 


Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile 
                325                 330                 335     


Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys 
            340                 345                 350         


Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys 
        355                 360                 365             


Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys 
    370                 375                 380                 


Glu Ile Ser Gln Glu Ala Arg Glu Met Ala Ser Ala Ala Arg Leu Thr 
385                 390                 395                 400 


Met Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val 
                405                 410                 415     


Glu Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile 
            420                 425                 430         


Ser Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln 
        435                 440                 445             


Arg Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met 
    450                 455                 460                 


Val Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Thr Gln 
465                 470                 475                 480 


Gly Glu Arg Gly Leu Lys Lys Met Leu Arg Thr Cys Ala Val His Ile 
                485                 490                 495     


Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu Asp 
            500                 505                 510         


Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly Asn 
        515                 520                 525             


Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His Phe 
    530                 535                 540                 


His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu Ala 
545                 550                 555                 560 


Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg Lys Gly His Phe 
                565                 570                 575     


Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn Lys 
            580                 585                 590         


Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu Gln 
        595                 600                 605             


Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly Met 
    610                 615                 620                 


Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser Phe 
625                 630                 635                 640 


Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro Gly 
                645                 650                 655     


Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro Leu 
            660                 665                 670         


Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
        675                 680         


<210>  35
<211>  606
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin3-TRIM21-RING-RING-PRYSPRY

<400>  35

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met Gln Ile Phe Val 
145                 150                 155                 160 


Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp 
                165                 170                 175     


Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro 
            180                 185                 190         


Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly 
        195                 200                 205             


Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu 
    210                 215                 220                 


Val Leu Arg Leu Arg Ala Ala Met Ala Ser Ala Ala Arg Leu Thr Met 
225                 230                 235                 240 


Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu 
                245                 250                 255     


Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser 
            260                 265                 270         


Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg 
        275                 280                 285             


Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val 
    290                 295                 300                 


Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu Met Ala Ser Ala 
305                 310                 315                 320 


Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu 
                325                 330                 335     


Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys 
            340                 345                 350         


Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro 
        355                 360                 365             


Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln 
    370                 375                 380                 


Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg 
385                 390                 395                 400 


Glu Gly Thr Gln Gly Glu Arg Gly Leu Lys Lys Met Leu Arg Thr Cys 
                405                 410                 415     


Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile 
            420                 425                 430         


Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser 
        435                 440                 445             


Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly 
    450                 455                 460                 


Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr 
465                 470                 475                 480 


Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg 
                485                 490                 495     


Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp 
            500                 505                 510         


Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro 
        515                 520                 525             


Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr 
    530                 535                 540                 


Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu 
545                 550                 555                 560 


Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe 
                565                 570                 575     


Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr 
            580                 585                 590         


Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
        595                 600                 605     


<210>  36
<211>  530
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pOP-TG-Ubiquitin2-TRIM21-RING-RING-PRYSPRY

<400>  36

Gly Ser His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Ala Ser Ala Ala 
145                 150                 155                 160 


Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro Ile Cys Leu Asp 
                165                 170                 175     


Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His Ser Phe Cys Gln 
            180                 185                 190         


Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser Val Cys Pro Val 
        195                 200                 205             


Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro Asn Arg Gln Leu 
    210                 215                 220                 


Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln Glu Ala Arg Glu 
225                 230                 235                 240 


Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys 
                245                 250                 255     


Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly 
            260                 265                 270         


His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly 
        275                 280                 285             


Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg 
    290                 295                 300                 


Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser 
305                 310                 315                 320 


Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Gly Leu Lys Lys Met 
                325                 330                 335     


Leu Arg Thr Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn 
            340                 345                 350         


Pro Trp Leu Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp 
        355                 360                 365             


Thr Gln Gln Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro 
    370                 375                 380                 


Met Val Leu Gly Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu 
385                 390                 395                 400 


Val Asp Val Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp 
                405                 410                 415     


Ser Val Arg Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe 
            420                 425                 430         


Trp Thr Ile Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr 
        435                 440                 445             


Pro Gln Thr Pro Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile 
    450                 455                 460                 


Phe Leu Asp Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp 
465                 470                 475                 480 


His Gly Ser Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro 
                485                 490                 495     


Leu Arg Pro Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr 
            500                 505                 510         


Ala Pro Leu Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr 
        515                 520                 525             


Asp Tyr 
    530 


<210>  37
<211>  554
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HLTV-Ubiquitin-TRIM21

<400>  37

Gly Gly Ser Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Cys Ala Val His Gly Glu 
                165                 170                 175     


Arg Leu His Leu Phe Cys Glu Lys Asp Gly Lys Ala Leu Cys Trp Val 
            180                 185                 190         


Cys Ala Gln Ser Arg Lys His Arg Asp His Ala Met Val Pro Leu Glu 
        195                 200                 205             


Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu Gln Val Ala Leu Gly Glu 
    210                 215                 220                 


Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys Leu Glu Val Glu Ile Ala 
225                 230                 235                 240 


Ile Lys Arg Ala Asp Trp Lys Lys Thr Val Glu Thr Gln Lys Ser Arg 
                245                 250                 255     


Ile His Ala Glu Phe Val Gln Gln Lys Asn Phe Leu Val Glu Glu Glu 
            260                 265                 270         


Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp Glu Arg Glu Gln Leu Arg 
        275                 280                 285             


Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala Gln Gln Ser Gln Ala Leu 
    290                 295                 300                 


Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg Cys His Ser Ser Ala Leu 
305                 310                 315                 320 


Glu Leu Leu Gln Glu Val Ile Ile Val Leu Glu Arg Ser Glu Ser Trp 
                325                 330                 335     


Asn Leu Lys Asp Leu Asp Ile Thr Ser Pro Glu Leu Arg Ser Val Cys 
            340                 345                 350         


His Val Pro Gly Leu Lys Lys Met Leu Arg Thr Cys Ala Val His Ile 
        355                 360                 365             


Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu Ile Leu Ser Glu Asp 
    370                 375                 380                 


Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln Ser Ile Pro Gly Asn 
385                 390                 395                 400 


Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu Gly Ala Gln His Phe 
                405                 410                 415     


His Ser Gly Lys His Tyr Trp Glu Val Asp Val Thr Gly Lys Glu Ala 
            420                 425                 430         


Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg Arg Lys Gly His Phe 
        435                 440                 445             


Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile Trp Leu Trp Asn Lys 
    450                 455                 460                 


Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr Pro Leu His Leu Gln 
465                 470                 475                 480 


Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp Tyr Glu Ala Gly Met 
                485                 490                 495     


Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser Leu Ile Tyr Ser Phe 
            500                 505                 510         


Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro Phe Phe Ser Pro Gly 
        515                 520                 525             


Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu Thr Leu Cys Pro Leu 
    530                 535                 540                 


Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
545                 550                 


<210>  38
<211>  639
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HLTV-Ubiquitin-TRIM21-RING-TRIM21

<400>  38

Gly Gly His Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile 
1               5                   10                  15      


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
            20                  25                  30          


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45              


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
    50                  55                  60                  


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Met 
65                  70                  75                  80  


Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu Glu Val Thr Cys Pro 
                85                  90                  95      


Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser Ile Glu Cys Gly His 
            100                 105                 110         


Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly Lys Gly Gly Gly Ser 
        115                 120                 125             


Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu Lys Asn Leu Arg Pro 
    130                 135                 140                 


Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu Lys Glu Ile Ser Gln 
145                 150                 155                 160 


Glu Ala Arg Glu Met Ala Ser Ala Ala Arg Leu Thr Met Met Trp Glu 
                165                 170                 175     


Glu Val Thr Cys Pro Ile Cys Leu Asp Pro Phe Val Glu Pro Val Ser 
            180                 185                 190         


Ile Glu Cys Gly His Ser Phe Cys Gln Glu Cys Ile Ser Gln Val Gly 
        195                 200                 205             


Lys Gly Gly Gly Ser Val Cys Pro Val Cys Arg Gln Arg Phe Leu Leu 
    210                 215                 220                 


Lys Asn Leu Arg Pro Asn Arg Gln Leu Ala Asn Met Val Asn Asn Leu 
225                 230                 235                 240 


Lys Glu Ile Ser Gln Glu Ala Arg Glu Gly Thr Gln Gly Glu Arg Cys 
                245                 250                 255     


Ala Val His Gly Glu Arg Leu His Leu Phe Cys Glu Lys Asp Gly Lys 
            260                 265                 270         


Ala Leu Cys Trp Val Cys Ala Gln Ser Arg Lys His Arg Asp His Ala 
        275                 280                 285             


Met Val Pro Leu Glu Glu Ala Ala Gln Glu Tyr Gln Glu Lys Leu Gln 
    290                 295                 300                 


Val Ala Leu Gly Glu Leu Arg Arg Lys Gln Glu Leu Ala Glu Lys Leu 
305                 310                 315                 320 


Glu Val Glu Ile Ala Ile Lys Arg Ala Asp Trp Lys Lys Thr Val Glu 
                325                 330                 335     


Thr Gln Lys Ser Arg Ile His Ala Glu Phe Val Gln Gln Lys Asn Phe 
            340                 345                 350         


Leu Val Glu Glu Glu Gln Arg Gln Leu Gln Glu Leu Glu Lys Asp Glu 
        355                 360                 365             


Arg Glu Gln Leu Arg Ile Leu Gly Glu Lys Glu Ala Lys Leu Ala Gln 
    370                 375                 380                 


Gln Ser Gln Ala Leu Gln Glu Leu Ile Ser Glu Leu Asp Arg Arg Cys 
385                 390                 395                 400 


His Ser Ser Ala Leu Glu Leu Leu Gln Glu Val Ile Ile Val Leu Glu 
                405                 410                 415     


Arg Ser Glu Ser Trp Asn Leu Lys Asp Leu Asp Ile Thr Ser Pro Glu 
            420                 425                 430         


Leu Arg Ser Val Cys His Val Pro Gly Leu Lys Lys Met Leu Arg Thr 
        435                 440                 445             


Cys Ala Val His Ile Thr Leu Asp Pro Asp Thr Ala Asn Pro Trp Leu 
    450                 455                 460                 


Ile Leu Ser Glu Asp Arg Arg Gln Val Arg Leu Gly Asp Thr Gln Gln 
465                 470                 475                 480 


Ser Ile Pro Gly Asn Glu Glu Arg Phe Asp Ser Tyr Pro Met Val Leu 
                485                 490                 495     


Gly Ala Gln His Phe His Ser Gly Lys His Tyr Trp Glu Val Asp Val 
            500                 505                 510         


Thr Gly Lys Glu Ala Trp Asp Leu Gly Val Cys Arg Asp Ser Val Arg 
        515                 520                 525             


Arg Lys Gly His Phe Leu Leu Ser Ser Lys Ser Gly Phe Trp Thr Ile 
    530                 535                 540                 


Trp Leu Trp Asn Lys Gln Lys Tyr Glu Ala Gly Thr Tyr Pro Gln Thr 
545                 550                 555                 560 


Pro Leu His Leu Gln Val Pro Pro Cys Gln Val Gly Ile Phe Leu Asp 
                565                 570                 575     


Tyr Glu Ala Gly Met Val Ser Phe Tyr Asn Ile Thr Asp His Gly Ser 
            580                 585                 590         


Leu Ile Tyr Ser Phe Ser Glu Cys Ala Phe Thr Gly Pro Leu Arg Pro 
        595                 600                 605             


Phe Phe Ser Pro Gly Phe Asn Asp Gly Gly Lys Asn Thr Ala Pro Leu 
    610                 615                 620                 


Thr Leu Cys Pro Leu Asn Ile Gly Ser Gln Gly Ser Thr Asp Tyr 
625                 630                 635                 


<210>  39
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  crRNA

<400>  39
atgctcacag gctccacgaa                                                   20


<210>  40
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker

<400>  40

Gly Thr Gln Gly Glu Arg Gly Leu Lys Lys Met Leu Arg Thr Cys 
1               5                   10                  15  


