                         SEQUENCE LISTING

<110>  Helmholtz Zentrum fuer Infektionsforschung GmbH
 
<120>  NOVEL DAROBACTIN DERIVATIVES

<130>  23813-PCT

<150>  EP 21 158 189
<151>  2021-02-19

<150>  EP 21 187 173
<151>  2021-07-22

<160>  89    

<170>  PatentIn version 3.5

<210>  1
<211>  8131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Biosynthetic Gene Cluster (BGC)


<220>
<221>  misc_feature
<222>  (1767)..(1769)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (1773)..(1784)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (2073)..(2075)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (2079)..(2090)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<400>  1
cgcgcgcgat tctaaccggt ataagaaata aagtggagcg ccggcgatta tccctaggtc       60

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg      120

cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga      180

cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca      240

cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac      300

atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc      360

cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg      420

cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac      480

tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc      540

agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt      600

gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga      660

aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag      720

tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc      780

cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc      840

gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg      900

cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg      960

actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg     1020

ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg     1080

aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca     1140

cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt     1200

tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg     1260

aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc     1320

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa     1380

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata     1440

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag     1500

aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga     1560

gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata     1620

tgcataatac cttaaatgaa accgttaaaa ctcaagaagc actcaattct cttgctgcat     1680

cattcaaaga gactgaactc tcaattactg ataaagcact aaacgaatta agcaataaac     1740

ctaagatccc tgagatcacg gcctggnnnt ggnnnnnnnn nnnncaggaa atttaaagct     1800

tatcccatca ggttatttta ttttcctgaa aaaacaatgt ccattgggat aaacaatcaa     1860

gttgttcagt taaaataaat aattttttga accttaatca tagggtaatc ctatgtatta     1920

tccgggatta ccctaaatac tttcataaag taaatataaa aatttgaata atacagataa     1980

gtttttttaa cctgaatgga aggtttgtat tttatcatta aacacaacca ccttttacca     2040

ctaatattat tggatttttt catttcaaga tgaaaatata atagatttca gtaactaaaa     2100

agggtaatag cctgtcccag caggtgttac tgatgcaggc aaataaaata gccctaatag     2160

gatgggcttt aagtaaggaa cggtagccat aactgcagtc ctgaggatcc ccaccctgca     2220

agggcccgga accgcaaggt tccgggccct tcgcctttta tcaggttatt tgcggccgct     2280

ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct     2340

gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat     2400

ctgatcaaga gacaggataa ggaggtacag attatgaatg ctattaacat ctcagaatcc     2460

atgaaacgtt tcaatatatt ggtatcgata ctctcagtat caatagcaat attagcaatt     2520

tatttacttt ctactgcctt ctattcagat gctcgagttg ataactatta tcaagataat     2580

ggcaacattt accgggtgga aaccaccttt aatttgccca atggagaaaa agtgagatcg     2640

gcaaaatcgc cattgccgct catagacgag ctagaaaaag ataaaaggat aaatcgtgca     2700

gactactttt tcaaacttaa cacgacgatt gagttacaag gaaaaaagat caccaaagtt     2760

cccgtgtttg cagtcagtca tcaattcctg aataccctgt ctccctttaa gaaaatcgat     2820

aatatcctgg gagcaaatga gatttacatc acaaaagagt tcaataatcg ctatctcggc     2880

ctggaaaatc ccaaaggaaa atctattgtt ctggataatg aaacctatat catcaaggat     2940

atcgtagaaa aacgtcatga ttcgagtctt aatatgaatg ctattatttc atttaagcca     3000

acacttatca aaaactacaa taaagaaata ttcaattggt atgatacaca tgcttatatt     3060

tttataaaac tctctgatga aaaatcaata tccaatcgcg atgcgcttct taatcatatt     3120

attgcaacca aagcccccaa tcttccaggc gctccattta ctgccagtga atttattcac     3180

ctatcattaa aaaaatcaaa agatattcat taccaagatg gattgcctga tgaaatttct     3240

attggtttat caaaggagat aatttatagc tcatatatcg cgttcgcatt tatcttattt     3300

tccacattaa ccaactatta taatttaact tctgctgatt cagcagaaaa aagggatatt     3360

tatcagttaa agaaagcgct aggcgcttca actttcaata ttatttgcga ttcactgccg     3420

gccatgttga tcaaaataat cctttccacc cttatctttt tgttacttat cataactata     3480

tttaatgtct ctctctatat aaataatata tttaccctat tagaagcaga cagtttaata     3540

tatctaggga tattttcagt tgttattgcc tttatcttta tttcatcaat acatttttca     3600

ttctcattac gtttttatat ttatcaaaga aacagaaaaa tggatatgag atatgaatca     3660

acccttgcct cttggtttag gaaatcatca ttggctattc aattacttgt ttccggaata     3720

tctatttatt tagtcaccgg attaaccaat caatatattc accttgttga tttacatact     3780

ccttatgata atgccaagac aatatcaata gcaattaata acgataataa taaaaaatca     3840

actattgatg aattaagcta tgagtttatc agaaaataca atacttcaaa aataaccctc     3900

agtaattggc gaccatttga tatgtcaaga gaaaatatca ctattaatta ttccggccaa     3960

caaaacagga ataactatat ctcttcaaat gttattactg cggatgaaaa tttcactgat     4020

gtgtggagaa tgaagatatt agccggtggt gataaacgag tatatcagag tgataatgct     4080

gatgtagtcc atgcattagt gacaaaagaa tttttaaaac agaatggaat tctcaaatat     4140

gatgatattt ttaacaacta ctattggtac acaatggagg ataaaaagat ccaaatcaga     4200

tttattcaga ttatagatga tttcaattta ggtgctgtag atgaaccatt caggccaata     4260

gttgttttca tcaaaaaaga tcatggaaaa tatgcttcgc tgaatttaaa caatatgaaa     4320

aacctttctc cggtacttaa cgaactatca aaacagggtt ttgataataa tgaaattgat     4380

ttaaccagtc acttatttaa ctcttattat gataattatt taaaagtaat aaaattatca     4440

tctgccgtcg ctattttctc ggttctatta ttagtcatcg cctgctttac taccagcatg     4500

actgacttta acgcgatgaa aagggagtta agtataatgg aatctattgg tggttctatt     4560

tacaccaatc tcattcactt tttatcgaaa agtgtcatcc cgataatcgc atcaattttc     4620

gtggcatata tatcaggaag aactatttta gtttatctgc ttgatgaata tagtgtgatt     4680

tacaccgatt cttttgtata cagtgctatc gccctatctg ctacaatgat attcaccgtt     4740

attataatga tgactaccta tttggtgaat tatagaaaat tatccgccac taaatttttg     4800

tgagattaat tatggatata gaaatcagaa aaaaaggcat acataacaga gagaaattac     4860

tcattctcat tttctttatc gcttttattt catttgttgt atttattatc tattatgtcg     4920

tttcggctaa agacgctttc gtgaatgaaa atgaagttac ccagtttcag gtcagcagca     4980

aagtagatag tgatattctg aaaaccaggg cagttgtggt tcctgataaa agtgtctcta     5040

tctcaaccga gataggggga atcgttaccg atataatgaa aaaaccatca caagatgttt     5100

taaagaatga agaaatcgtg aaattatcta attttaattt cacattgaat aattcttcga     5160

tgttagcgga cgtgacagat aaactcaata atttaataaa cataagaata aacttgcaat     5220

ctgactatcg ggatatcaat aaccgattct tagaagccgc caaagaacta aaagaagtag     5280

aagataaatt aaagcgatat cacgatcttg tacataaaaa ttatatttct aaagaaacta     5340

tgtccgattt aaggattaaa agagattatt ggcacgatgt ttatctattc tacaaaaaat     5400

taaaaaatga taaagatcgt gatatatcaa aacagttaaa ggaaatcgac gagtttgtcg     5460

aaaaacagag aaaactctct gacatcatcg aaaatggttt cgagcagctt tcaataaaat     5520

cccctatcgc cggtaatatc agttcattag atttaatttt aggacaacgg ttaaaacctg     5580

gcgataaaat agctattgtt gatgatttat caaactttta ttttgaatca gaaatcaacg     5640

aatactattt aaacaaaata acataccatt catctgccag tttaatctat aacaatggaa     5700

aaataccttt actggtgaaa ttaatttcat ctgaagtgaa taatggaaca ttcaaagtca     5760

gatttgaatt ggaagataaa aaaacaatta acttcaaacg ggggcaatcc gttgatgtca     5820

tgattaaact tgatgaagat cggaaaatat tctccgtacc ttcatctatg gtgttttcta     5880

ttgacaataa aaactatgtg tttgtttacc atccagataa aaaaattgcc cgacgtattc     5940

aagtttccac cggccaagat aatggttctg atattgagat caaaagtggt gttactgaag     6000

gacaaacgct cgttagcttt ggtaagaata aattagttaa taatgataca gtaaggattg     6060

aataaaatga ttagcatgat gaatatctgt aaatcatata aaacgaaatt cattcaaaca     6120

aatgttttga atgacataaa tctcaatata gataaaggcg aatttatttc tataatggga     6180

gcgtcagggt cagggaaatc aacattactg aatgtcattg gcatgtttga aacaatcgat     6240

agcggtcaat taacactgaa caatagcaat attttaacaa tgaaatattc tgaaaaaata     6300

tcattcagac gagaatttat tggttatatt tttcaatcat tcaatctgtt acccaatttg     6360

acggtatttg aaaacataga gttaccgtta aaatacagag gttttcctaa aaaacagcgg     6420

aaaagcaaag tatttgatgc aataaatagt tttggattag aaaatcggga aaatcataaa     6480

ccgatacaat tatctggtgg acaacagcaa cgtgttgcta tcgccagagc aatgatagca     6540

aaccccacca ttttattagc tgatgaacca acggggaacc tggatagcgt aaatggtcag     6600

aatattttat cttcacttaa ggagctgaat gaaaacggta cgacgattgt tatggtaacc     6660

cattccgtag aagcggcagc gttttctgac agaatattaa cgatgaggga tggccacttg     6720

cttgtttgag cataaataac ataataaagg agttctttat ggacacaata atccccataa     6780

aatatttaga ttcagacgaa tcatcgattc ttaagaaatc atctaaaatt aactacaggc     6840

aattagcttg cagaattatc ggtgaaatct ccgccgaaaa aatattagat gatgatgaac     6900

tggctttata taataaagaa atcagtatac atttcagccc tgaaattatt aatgctaata     6960

aattagttgt ggttgtgaaa gccaccaggc tttgcaattt aagatgcact tattgtcact     7020

cctgggcaga aggaaaagga aataccttaa cattcttcaa tttaatgcgt tccattcacc     7080

gtttcttatc cctaccgaat atcaagcgat ttgaattcgt ctggcatggc ggcgaagtaa     7140

cgttgttgaa tgttaattac tttaagaaac tcatctggtt acaggaacaa tttaaaaaac     7200

cggatcaagt tatcaccaat tcggtacaga caaatgccgt caatattcct gaagattggt     7260

tagtgttcct caaaggtatt ggaatggggg taggaataag cgttgatggt attccggaaa     7320

tacacgatag caggagatta gattacagag gaaggccaac atcccataaa gtcgcggcaa     7380

gtatgaaaaa gttaagaagt tatggcatac cttacggtgc gcttatcgtc gtcgaccgcg     7440

atgtttatga atcaaatata gaaaaaatgc tctcttattt ttacgaaatc ggtttaacgg     7500

atattgaatt tctgaatatt gtcccagata accgatgcca gccgggtgat gatcctggag     7560

gaagttatat aacttaccat aactatatta atttcctttc taaggttttc cgtgtctggt     7620

ggaatggtta tcaaggcaaa atcaatattc gcttgtttga cggatttatt gacagtatca     7680

aatcgtccca aaagaaaatg tcagattgtt attgggcggg taactgttct caggaaataa     7740

tcacattaga acctaatggt acggtatcag catgtgataa atatgttggt gctgaaggga     7800

ataattatgg ttcgattatt gataatgatc ttgggaattt actatctaaa tcaaatacaa     7860

ataaggatca tcttaaagag gaaatggaat cttatgaaaa aatgcatcaa tgtaaatggt     7920

ttcatttgtg taatggtgga tgcccacacg atcgagtgac caacaggaag cacaatccaa     7980

attatgatgg ttcatgttgt ggaaccggcg gtttgttgga gacaataaaa caaaccatcg     8040

cggcgtaaat tctaactagt tcctgattaa ttaaccaccc tgcaagggcc cggaaccgca     8100

aggttccggg cccttcgcct tttcgtcgac g                                    8131


<210>  2
<211>  21
<212>  DNA
<213>  Photorhabdus khanii


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin A

<400>  2
tgg aac tgg tca aaa agc ttc                                             21
Trp Asn Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  3
<211>  7
<212>  PRT
<213>  Photorhabdus khanii

<400>  3

Trp Asn Trp Ser Lys Ser Phe 
1               5           


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin B


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin B

<400>  4
tgg aac tgg acc aaa cga ttc                                             21
Trp Asn Trp Thr Lys Arg Phe                                               
1               5                                                         


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Trp Asn Trp Thr Lys Arg Phe 
1               5           


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin C


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin C

<400>  6
tgg tca tgg tca aga agc ttc                                             21
Trp Ser Trp Ser Arg Ser Phe                                               
1               5                                                         


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Trp Ser Trp Ser Arg Ser Phe 
1               5           


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin D


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin D

<400>  8
tgg aac tgg tca aga agc ttc                                             21
Trp Asn Trp Ser Arg Ser Phe                                               
1               5                                                         


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Trp Asn Trp Ser Arg Ser Phe 
1               5           


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin E


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin E

<400>  10
tgg tca tgg tca aaa agc ttc                                             21
Trp Ser Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Trp Ser Trp Ser Lys Ser Phe 
1               5           


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 1


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 1

<400>  12
tgg tca tgg acc aaa cga ttc                                             21
Trp Ser Trp Thr Lys Arg Phe                                               
1               5                                                         


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Trp Ser Trp Thr Lys Arg Phe 
1               5           


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 2


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 2

<400>  14
tgg tca tgg acc aga agc ttc                                             21
Trp Ser Trp Thr Arg Ser Phe                                               
1               5                                                         


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Trp Ser Trp Thr Arg Ser Phe 
1               5           


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 4


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 4

<400>  16
tgg aac tgg acc aaa agc ttc                                             21
Trp Asn Trp Thr Lys Ser Phe                                               
1               5                                                         


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Trp Asn Trp Thr Lys Ser Phe 
1               5           


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 5


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 5

<400>  18
tgg tgc tgg tca aaa agc ttc                                             21
Trp Cys Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19

Trp Cys Trp Ser Lys Ser Phe 
1               5           


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 6


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 6

<400>  20
tgg aac tgg tgc aaa agc ttc                                             21
Trp Asn Trp Cys Lys Ser Phe                                               
1               5                                                         


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21

Trp Asn Trp Cys Lys Ser Phe 
1               5           


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 7


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 7

<400>  22
tgg tgc tgg tgc aaa agc ttc                                             21
Trp Cys Trp Cys Lys Ser Phe                                               
1               5                                                         


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23

Trp Cys Trp Cys Lys Ser Phe 
1               5           


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 8


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 8

<400>  24
tgg aac tgg tca aaa tgc ttc                                             21
Trp Asn Trp Ser Lys Cys Phe                                               
1               5                                                         


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  25

Trp Asn Trp Ser Lys Cys Phe 
1               5           


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 9


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 9

<400>  26
tgg aac tgg tca aaa agc tgg                                             21
Trp Asn Trp Ser Lys Ser Trp                                               
1               5                                                         


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27

Trp Asn Trp Ser Lys Ser Trp 
1               5           


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 10


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 10

<400>  28
tgg aac tgg tca aaa agc tac                                             21
Trp Asn Trp Ser Lys Ser Tyr                                               
1               5                                                         


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  29

Trp Asn Trp Ser Lys Ser Tyr 
1               5           


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 11


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 11

<400>  30
tgg cag tgg tca aaa agc ttc                                             21
Trp Gln Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  31
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  31

Trp Gln Trp Ser Lys Ser Phe 
1               5           


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 12


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 12

<400>  32
tgg acc tgg tca aaa agc ttc                                             21
Trp Thr Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  33

Trp Thr Trp Ser Lys Ser Phe 
1               5           


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 13


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 13

<400>  34
tgg gca tgg tca aaa agc ttc                                             21
Trp Ala Trp Ser Lys Ser Phe                                               
1               5                                                         


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  35

Trp Ala Trp Ser Lys Ser Phe 
1               5           


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 14


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 14

<400>  36
tgg aac tgg gca aaa agc ttc                                             21
Trp Asn Trp Ala Lys Ser Phe                                               
1               5                                                         


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  37

Trp Asn Trp Ala Lys Ser Phe 
1               5           


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 15


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 15

<400>  38
tgg aac tgg tca gca agc ttc                                             21
Trp Asn Trp Ser Ala Ser Phe                                               
1               5                                                         


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  39

Trp Asn Trp Ser Ala Ser Phe 
1               5           


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 16


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 16

<400>  40
tgg aac tgg tca aaa gct ttc                                             21
Trp Asn Trp Ser Lys Ala Phe                                               
1               5                                                         


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  41

Trp Asn Trp Ser Lys Ala Phe 
1               5           


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 17


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 17

<400>  42
tgg aac tgg tca aaa agc gca                                             21
Trp Asn Trp Ser Lys Ser Ala                                               
1               5                                                         


<210>  43
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  43

Trp Asn Trp Ser Lys Ser Ala 
1               5           


<210>  44
<211>  8131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Biosynthetic Gene Cluster (BGC)


<220>
<221>  misc_feature
<222>  (1773)..(1775)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (1779)..(1781)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<400>  44
cgcgcgcgat tctaaccggt ataagaaata aagtggagcg ccggcgatta tccctaggtc       60

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg      120

cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga      180

cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca      240

cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac      300

atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc      360

cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg      420

cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac      480

tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc      540

agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt      600

gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga      660

aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag      720

tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc      780

cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc      840

gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg      900

cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg      960

actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg     1020

ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg     1080

aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca     1140

cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt     1200

tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg     1260

aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc     1320

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa     1380

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata     1440

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag     1500

aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga     1560

gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata     1620

tgcataatac cttaaatgaa accgttaaaa ctcaagaagc actcaattct cttgctgcat     1680

cattcaaaga gactgaactc tcaattactg ataaagcact aaacgaatta agcaataaac     1740

ctaagatccc tgagatcacg gcctggaact ggnnnaaann ntggcaggaa atttaaagct     1800

tatcccatca ggttatttta ttttcctgaa aaaacaatgt ccattgggat aaacaatcaa     1860

gttgttcagt taaaataaat aattttttga accttaatca tagggtaatc ctatgtatta     1920

tccgggatta ccctaaatac tttcataaag taaatataaa aatttgaata atacagataa     1980

gtttttttaa cctgaatgga aggtttgtat tttatcatta aacacaacca ccttttacca     2040

ctaatattat tggatttttt catttcaaga tgaaaatata atagatttca gtaactaaaa     2100

agggtaatag cctgtcccag caggtgttac tgatgcaggc aaataaaata gccctaatag     2160

gatgggcttt aagtaaggaa cggtagccat aactgcagtc ctgaggatcc ccaccctgca     2220

agggcccgga accgcaaggt tccgggccct tcgcctttta tcaggttatt tgcggccgct     2280

ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct     2340

gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat     2400

ctgatcaaga gacaggataa ggaggtacag attatgaatg ctattaacat ctcagaatcc     2460

atgaaacgtt tcaatatatt ggtatcgata ctctcagtat caatagcaat attagcaatt     2520

tatttacttt ctactgcctt ctattcagat gctcgagttg ataactatta tcaagataat     2580

ggcaacattt accgggtgga aaccaccttt aatttgccca atggagaaaa agtgagatcg     2640

gcaaaatcgc cattgccgct catagacgag ctagaaaaag ataaaaggat aaatcgtgca     2700

gactactttt tcaaacttaa cacgacgatt gagttacaag gaaaaaagat caccaaagtt     2760

cccgtgtttg cagtcagtca tcaattcctg aataccctgt ctccctttaa gaaaatcgat     2820

aatatcctgg gagcaaatga gatttacatc acaaaagagt tcaataatcg ctatctcggc     2880

ctggaaaatc ccaaaggaaa atctattgtt ctggataatg aaacctatat catcaaggat     2940

atcgtagaaa aacgtcatga ttcgagtctt aatatgaatg ctattatttc atttaagcca     3000

acacttatca aaaactacaa taaagaaata ttcaattggt atgatacaca tgcttatatt     3060

tttataaaac tctctgatga aaaatcaata tccaatcgcg atgcgcttct taatcatatt     3120

attgcaacca aagcccccaa tcttccaggc gctccattta ctgccagtga atttattcac     3180

ctatcattaa aaaaatcaaa agatattcat taccaagatg gattgcctga tgaaatttct     3240

attggtttat caaaggagat aatttatagc tcatatatcg cgttcgcatt tatcttattt     3300

tccacattaa ccaactatta taatttaact tctgctgatt cagcagaaaa aagggatatt     3360

tatcagttaa agaaagcgct aggcgcttca actttcaata ttatttgcga ttcactgccg     3420

gccatgttga tcaaaataat cctttccacc cttatctttt tgttacttat cataactata     3480

tttaatgtct ctctctatat aaataatata tttaccctat tagaagcaga cagtttaata     3540

tatctaggga tattttcagt tgttattgcc tttatcttta tttcatcaat acatttttca     3600

ttctcattac gtttttatat ttatcaaaga aacagaaaaa tggatatgag atatgaatca     3660

acccttgcct cttggtttag gaaatcatca ttggctattc aattacttgt ttccggaata     3720

tctatttatt tagtcaccgg attaaccaat caatatattc accttgttga tttacatact     3780

ccttatgata atgccaagac aatatcaata gcaattaata acgataataa taaaaaatca     3840

actattgatg aattaagcta tgagtttatc agaaaataca atacttcaaa aataaccctc     3900

agtaattggc gaccatttga tatgtcaaga gaaaatatca ctattaatta ttccggccaa     3960

caaaacagga ataactatat ctcttcaaat gttattactg cggatgaaaa tttcactgat     4020

gtgtggagaa tgaagatatt agccggtggt gataaacgag tatatcagag tgataatgct     4080

gatgtagtcc atgcattagt gacaaaagaa tttttaaaac agaatggaat tctcaaatat     4140

gatgatattt ttaacaacta ctattggtac acaatggagg ataaaaagat ccaaatcaga     4200

tttattcaga ttatagatga tttcaattta ggtgctgtag atgaaccatt caggccaata     4260

gttgttttca tcaaaaaaga tcatggaaaa tatgcttcgc tgaatttaaa caatatgaaa     4320

aacctttctc cggtacttaa cgaactatca aaacagggtt ttgataataa tgaaattgat     4380

ttaaccagtc acttatttaa ctcttattat gataattatt taaaagtaat aaaattatca     4440

tctgccgtcg ctattttctc ggttctatta ttagtcatcg cctgctttac taccagcatg     4500

actgacttta acgcgatgaa aagggagtta agtataatgg aatctattgg tggttctatt     4560

tacaccaatc tcattcactt tttatcgaaa agtgtcatcc cgataatcgc atcaattttc     4620

gtggcatata tatcaggaag aactatttta gtttatctgc ttgatgaata tagtgtgatt     4680

tacaccgatt cttttgtata cagtgctatc gccctatctg ctacaatgat attcaccgtt     4740

attataatga tgactaccta tttggtgaat tatagaaaat tatccgccac taaatttttg     4800

tgagattaat tatggatata gaaatcagaa aaaaaggcat acataacaga gagaaattac     4860

tcattctcat tttctttatc gcttttattt catttgttgt atttattatc tattatgtcg     4920

tttcggctaa agacgctttc gtgaatgaaa atgaagttac ccagtttcag gtcagcagca     4980

aagtagatag tgatattctg aaaaccaggg cagttgtggt tcctgataaa agtgtctcta     5040

tctcaaccga gataggggga atcgttaccg atataatgaa aaaaccatca caagatgttt     5100

taaagaatga agaaatcgtg aaattatcta attttaattt cacattgaat aattcttcga     5160

tgttagcgga cgtgacagat aaactcaata atttaataaa cataagaata aacttgcaat     5220

ctgactatcg ggatatcaat aaccgattct tagaagccgc caaagaacta aaagaagtag     5280

aagataaatt aaagcgatat cacgatcttg tacataaaaa ttatatttct aaagaaacta     5340

tgtccgattt aaggattaaa agagattatt ggcacgatgt ttatctattc tacaaaaaat     5400

taaaaaatga taaagatcgt gatatatcaa aacagttaaa ggaaatcgac gagtttgtcg     5460

aaaaacagag aaaactctct gacatcatcg aaaatggttt cgagcagctt tcaataaaat     5520

cccctatcgc cggtaatatc agttcattag atttaatttt aggacaacgg ttaaaacctg     5580

gcgataaaat agctattgtt gatgatttat caaactttta ttttgaatca gaaatcaacg     5640

aatactattt aaacaaaata acataccatt catctgccag tttaatctat aacaatggaa     5700

aaataccttt actggtgaaa ttaatttcat ctgaagtgaa taatggaaca ttcaaagtca     5760

gatttgaatt ggaagataaa aaaacaatta acttcaaacg ggggcaatcc gttgatgtca     5820

tgattaaact tgatgaagat cggaaaatat tctccgtacc ttcatctatg gtgttttcta     5880

ttgacaataa aaactatgtg tttgtttacc atccagataa aaaaattgcc cgacgtattc     5940

aagtttccac cggccaagat aatggttctg atattgagat caaaagtggt gttactgaag     6000

gacaaacgct cgttagcttt ggtaagaata aattagttaa taatgataca gtaaggattg     6060

aataaaatga ttagcatgat gaatatctgt aaatcatata aaacgaaatt cattcaaaca     6120

aatgttttga atgacataaa tctcaatata gataaaggcg aatttatttc tataatggga     6180

gcgtcagggt cagggaaatc aacattactg aatgtcattg gcatgtttga aacaatcgat     6240

agcggtcaat taacactgaa caatagcaat attttaacaa tgaaatattc tgaaaaaata     6300

tcattcagac gagaatttat tggttatatt tttcaatcat tcaatctgtt acccaatttg     6360

acggtatttg aaaacataga gttaccgtta aaatacagag gttttcctaa aaaacagcgg     6420

aaaagcaaag tatttgatgc aataaatagt tttggattag aaaatcggga aaatcataaa     6480

ccgatacaat tatctggtgg acaacagcaa cgtgttgcta tcgccagagc aatgatagca     6540

aaccccacca ttttattagc tgatgaacca acggggaacc tggatagcgt aaatggtcag     6600

aatattttat cttcacttaa ggagctgaat gaaaacggta cgacgattgt tatggtaacc     6660

cattccgtag aagcggcagc gttttctgac agaatattaa cgatgaggga tggccacttg     6720

cttgtttgag cataaataac ataataaagg agttctttat ggacacaata atccccataa     6780

aatatttaga ttcagacgaa tcatcgattc ttaagaaatc atctaaaatt aactacaggc     6840

aattagcttg cagaattatc ggtgaaatct ccgccgaaaa aatattagat gatgatgaac     6900

tggctttata taataaagaa atcagtatac atttcagccc tgaaattatt aatgctaata     6960

aattagttgt ggttgtgaaa gccaccaggc tttgcaattt aagatgcact tattgtcact     7020

cctgggcaga aggaaaagga aataccttaa cattcttcaa tttaatgcgt tccattcacc     7080

gtttcttatc cctaccgaat atcaagcgat ttgaattcgt ctggcatggc ggcgaagtaa     7140

cgttgttgaa tgttaattac tttaagaaac tcatctggtt acaggaacaa tttaaaaaac     7200

cggatcaagt tatcaccaat tcggtacaga caaatgccgt caatattcct gaagattggt     7260

tagtgttcct caaaggtatt ggaatggggg taggaataag cgttgatggt attccggaaa     7320

tacacgatag caggagatta gattacagag gaaggccaac atcccataaa gtcgcggcaa     7380

gtatgaaaaa gttaagaagt tatggcatac cttacggtgc gcttatcgtc gtcgaccgcg     7440

atgtttatga atcaaatata gaaaaaatgc tctcttattt ttacgaaatc ggtttaacgg     7500

atattgaatt tctgaatatt gtcccagata accgatgcca gccgggtgat gatcctggag     7560

gaagttatat aacttaccat aactatatta atttcctttc taaggttttc cgtgtctggt     7620

ggaatggtta tcaaggcaaa atcaatattc gcttgtttga cggatttatt gacagtatca     7680

aatcgtccca aaagaaaatg tcagattgtt attgggcggg taactgttct caggaaataa     7740

tcacattaga acctaatggt acggtatcag catgtgataa atatgttggt gctgaaggga     7800

ataattatgg ttcgattatt gataatgatc ttgggaattt actatctaaa tcaaatacaa     7860

ataaggatca tcttaaagag gaaatggaat cttatgaaaa aatgcatcaa tgtaaatggt     7920

ttcatttgtg taatggtgga tgcccacacg atcgagtgac caacaggaag cacaatccaa     7980

attatgatgg ttcatgttgt ggaaccggcg gtttgttgga gacaataaaa caaaccatcg     8040

cggcgtaaat tctaactagt tcctgattaa ttaaccaccc tgcaagggcc cggaaccgca     8100

aggttccggg cccttcgcct tttcgtcgac g                                    8131


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer - pr1dar_fw for overlap extension PCR

<400>  45
ggtgatgtcg gcgatatagg                                                   20


<210>  46
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer - pr3dartrp_fw for overlap extension PCR

<400>  46
tggcaggaaa tttaaagctt atcccat                                           27


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr4dar_rv for overlap extension PCR

<400>  47
tggggatcct caggactgca g                                                 21


<210>  48
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar22_rv containing mutations of D22

<400>  48
aaatttcctg ccaccgttta gtccagttcc                                        30


<210>  49
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar23_rv containing mutations of D23

<400>  49
aaatttcctg ccaatgttta gtccagttcc                                        30


<210>  50
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar24_rv containing mutations of D24

<400>  50
aaatttcctg ccatgattta gtccagttcc                                        30


<210>  51
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar25_rv containing mutations of D25

<400>  51
aaatttcctg ccacgcttta gtccagttcc                                        30


<210>  52
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar30_rv containing mutations of D30

<400>  52
aaatttcctg ccatgatttc gcccagttcc                                        30


<210>  53
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar31_rv containing mutations of D31

<400>  53
aaatttcctg ccaccgtttc gcccagttcc                                        30


<210>  54
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar32_rv containing mutations of D32

<400>  54
aaatttcctg ccaccgttta caccagttcc                                        30


<210>  55
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar33_rv containing mutations of D33

<400>  55
aaatttcctg ccatgattta caccagttcc                                        30


<210>  56
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar34_rv containing mutations of D34

<400>  56
aaatttcctg ccacgcttta caccagttcc                                        30


<210>  57
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar36_rv containing mutations of D36

<400>  57
aaatttcctg ccatttttta gtccagttcc                                        30


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar37_rv containing mutations of D37

<400>  58
aaatttcctg ccaagttttt gaccagttcc                                        30


<210>  59
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar38_rv containing mutations of D38

<400>  59
aaatttcctg ccaagtttta gtccagttcc                                        30


<210>  60
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence - Darobactin 9


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 9

<400>  60
tgg aac tgg tca aaa agc tgg                                             21
Trp Asn Trp Ser Lys Ser Trp                                               
1               5                                                         


<210>  61
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  61

Trp Asn Trp Ser Lys Ser Trp 
1               5           


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 22

<400>  62
tgg aac tgg act aaa cgg tgg                                             21
Trp Asn Trp Thr Lys Arg Trp                                               
1               5                                                         


<210>  63
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  63

Trp Asn Trp Thr Lys Arg Trp 
1               5           


<210>  64
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 23

<400>  64
tgg aac tgg act aaa cat tgg                                             21
Trp Asn Trp Thr Lys His Trp                                               
1               5                                                         


<210>  65
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  65

Trp Asn Trp Thr Lys His Trp 
1               5           


<210>  66
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 24

<400>  66
tgg aac tgg act aaa tca tgg                                             21
Trp Asn Trp Thr Lys Ser Trp                                               
1               5                                                         


<210>  67
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  67

Trp Asn Trp Thr Lys Ser Trp 
1               5           


<210>  68
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 25

<400>  68
tgg aac tgg act aaa gcg tgg                                             21
Trp Asn Trp Thr Lys Ala Trp                                               
1               5                                                         


<210>  69
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  69

Trp Asn Trp Thr Lys Ala Trp 
1               5           


<210>  70
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 30

<400>  70
tgg aac tgg gcg aaa tca tgg                                             21
Trp Asn Trp Ala Lys Ser Trp                                               
1               5                                                         


<210>  71
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  71

Trp Asn Trp Ala Lys Ser Trp 
1               5           


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 31

<400>  72
tgg aac tgg gcg aaa cgg tgg                                             21
Trp Asn Trp Ala Lys Arg Trp                                               
1               5                                                         


<210>  73
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  73

Trp Asn Trp Ala Lys Arg Trp 
1               5           


<210>  74
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 32

<400>  74
tgg aac tgg tgt aaa cgg tgg                                             21
Trp Asn Trp Cys Lys Arg Trp                                               
1               5                                                         


<210>  75
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  75

Trp Asn Trp Cys Lys Arg Trp 
1               5           


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 33

<400>  76
tgg aac tgg tgt aaa tca tgg                                             21
Trp Asn Trp Cys Lys Ser Trp                                               
1               5                                                         


<210>  77
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  77

Trp Asn Trp Cys Lys Ser Trp 
1               5           


<210>  78
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 34

<400>  78
tgg aac tgg tgt aaa gcg tgg                                             21
Trp Asn Trp Cys Lys Ala Trp                                               
1               5                                                         


<210>  79
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  79

Trp Asn Trp Cys Lys Ala Trp 
1               5           


<210>  80
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 36

<400>  80
tgg aac tgg act aaa aaa tgg                                             21
Trp Asn Trp Thr Lys Lys Trp                                               
1               5                                                         


<210>  81
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  81

Trp Asn Trp Thr Lys Lys Trp 
1               5           


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 37

<400>  82
tgg aac tgg tca aaa act tgg                                             21
Trp Asn Trp Ser Lys Thr Trp                                               
1               5                                                         


<210>  83
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  83

Trp Asn Trp Ser Lys Thr Trp 
1               5           


<210>  84
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propetide core sequence


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 38

<400>  84
tgg aac tgg act aaa act tgg                                             21
Trp Asn Trp Thr Lys Thr Trp                                               
1               5                                                         


<210>  85
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  85

Trp Asn Trp Thr Lys Thr Trp 
1               5           


<210>  86
<211>  8131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Biosynthetic Gene Cluster (BGC)


<220>
<221>  misc_feature
<222>  (1773)..(1775)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (1776)..(1778)
<223>  each nucleotide is a nucleotide which is part of a base triplet 
       coding for arginine or lysine, including agg, aga, aag, aaa, cga,
       cgc, cgg and cgt

<220>
<221>  misc_feature
<222>  (1779)..(1781)
<223>  n is a, g, c or t

       each nucleotide is a nucleotide which is part of a base triplet 
       coding for an amino acid

<220>
<221>  misc_feature
<222>  (1782)..(1784)
<223>  each nucleotide is a nucleotide which is part of a base triplet 
       coding for tryptophan, alanine or histidine, including tgg, gca, 
       gcc, gcg, gct, cac and cat

<400>  86
cgcgcgcgat tctaaccggt ataagaaata aagtggagcg ccggcgatta tccctaggtc       60

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg      120

cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga      180

cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca      240

cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac      300

atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc      360

cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg      420

cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac      480

tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc      540

agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt      600

gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga      660

aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag      720

tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc      780

cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc      840

gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg      900

cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg      960

actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg     1020

ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg     1080

aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca     1140

cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt     1200

tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg     1260

aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc     1320

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa     1380

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata     1440

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag     1500

aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga     1560

gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata     1620

tgcataatac cttaaatgaa accgttaaaa ctcaagaagc actcaattct cttgctgcat     1680

cattcaaaga gactgaactc tcaattactg ataaagcact aaacgaatta agcaataaac     1740

ctaagatccc tgagatcacg gcctggaact ggnnnnnnnn nnnncaggaa atttaaagct     1800

tatcccatca ggttatttta ttttcctgaa aaaacaatgt ccattgggat aaacaatcaa     1860

gttgttcagt taaaataaat aattttttga accttaatca tagggtaatc ctatgtatta     1920

tccgggatta ccctaaatac tttcataaag taaatataaa aatttgaata atacagataa     1980

gtttttttaa cctgaatgga aggtttgtat tttatcatta aacacaacca ccttttacca     2040

ctaatattat tggatttttt catttcaaga tgaaaatata atagatttca gtaactaaaa     2100

agggtaatag cctgtcccag caggtgttac tgatgcaggc aaataaaata gccctaatag     2160

gatgggcttt aagtaaggaa cggtagccat aactgcagtc ctgaggatcc ccaccctgca     2220

agggcccgga accgcaaggt tccgggccct tcgcctttta tcaggttatt tgcggccgct     2280

ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct     2340

gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat     2400

ctgatcaaga gacaggataa ggaggtacag attatgaatg ctattaacat ctcagaatcc     2460

atgaaacgtt tcaatatatt ggtatcgata ctctcagtat caatagcaat attagcaatt     2520

tatttacttt ctactgcctt ctattcagat gctcgagttg ataactatta tcaagataat     2580

ggcaacattt accgggtgga aaccaccttt aatttgccca atggagaaaa agtgagatcg     2640

gcaaaatcgc cattgccgct catagacgag ctagaaaaag ataaaaggat aaatcgtgca     2700

gactactttt tcaaacttaa cacgacgatt gagttacaag gaaaaaagat caccaaagtt     2760

cccgtgtttg cagtcagtca tcaattcctg aataccctgt ctccctttaa gaaaatcgat     2820

aatatcctgg gagcaaatga gatttacatc acaaaagagt tcaataatcg ctatctcggc     2880

ctggaaaatc ccaaaggaaa atctattgtt ctggataatg aaacctatat catcaaggat     2940

atcgtagaaa aacgtcatga ttcgagtctt aatatgaatg ctattatttc atttaagcca     3000

acacttatca aaaactacaa taaagaaata ttcaattggt atgatacaca tgcttatatt     3060

tttataaaac tctctgatga aaaatcaata tccaatcgcg atgcgcttct taatcatatt     3120

attgcaacca aagcccccaa tcttccaggc gctccattta ctgccagtga atttattcac     3180

ctatcattaa aaaaatcaaa agatattcat taccaagatg gattgcctga tgaaatttct     3240

attggtttat caaaggagat aatttatagc tcatatatcg cgttcgcatt tatcttattt     3300

tccacattaa ccaactatta taatttaact tctgctgatt cagcagaaaa aagggatatt     3360

tatcagttaa agaaagcgct aggcgcttca actttcaata ttatttgcga ttcactgccg     3420

gccatgttga tcaaaataat cctttccacc cttatctttt tgttacttat cataactata     3480

tttaatgtct ctctctatat aaataatata tttaccctat tagaagcaga cagtttaata     3540

tatctaggga tattttcagt tgttattgcc tttatcttta tttcatcaat acatttttca     3600

ttctcattac gtttttatat ttatcaaaga aacagaaaaa tggatatgag atatgaatca     3660

acccttgcct cttggtttag gaaatcatca ttggctattc aattacttgt ttccggaata     3720

tctatttatt tagtcaccgg attaaccaat caatatattc accttgttga tttacatact     3780

ccttatgata atgccaagac aatatcaata gcaattaata acgataataa taaaaaatca     3840

actattgatg aattaagcta tgagtttatc agaaaataca atacttcaaa aataaccctc     3900

agtaattggc gaccatttga tatgtcaaga gaaaatatca ctattaatta ttccggccaa     3960

caaaacagga ataactatat ctcttcaaat gttattactg cggatgaaaa tttcactgat     4020

gtgtggagaa tgaagatatt agccggtggt gataaacgag tatatcagag tgataatgct     4080

gatgtagtcc atgcattagt gacaaaagaa tttttaaaac agaatggaat tctcaaatat     4140

gatgatattt ttaacaacta ctattggtac acaatggagg ataaaaagat ccaaatcaga     4200

tttattcaga ttatagatga tttcaattta ggtgctgtag atgaaccatt caggccaata     4260

gttgttttca tcaaaaaaga tcatggaaaa tatgcttcgc tgaatttaaa caatatgaaa     4320

aacctttctc cggtacttaa cgaactatca aaacagggtt ttgataataa tgaaattgat     4380

ttaaccagtc acttatttaa ctcttattat gataattatt taaaagtaat aaaattatca     4440

tctgccgtcg ctattttctc ggttctatta ttagtcatcg cctgctttac taccagcatg     4500

actgacttta acgcgatgaa aagggagtta agtataatgg aatctattgg tggttctatt     4560

tacaccaatc tcattcactt tttatcgaaa agtgtcatcc cgataatcgc atcaattttc     4620

gtggcatata tatcaggaag aactatttta gtttatctgc ttgatgaata tagtgtgatt     4680

tacaccgatt cttttgtata cagtgctatc gccctatctg ctacaatgat attcaccgtt     4740

attataatga tgactaccta tttggtgaat tatagaaaat tatccgccac taaatttttg     4800

tgagattaat tatggatata gaaatcagaa aaaaaggcat acataacaga gagaaattac     4860

tcattctcat tttctttatc gcttttattt catttgttgt atttattatc tattatgtcg     4920

tttcggctaa agacgctttc gtgaatgaaa atgaagttac ccagtttcag gtcagcagca     4980

aagtagatag tgatattctg aaaaccaggg cagttgtggt tcctgataaa agtgtctcta     5040

tctcaaccga gataggggga atcgttaccg atataatgaa aaaaccatca caagatgttt     5100

taaagaatga agaaatcgtg aaattatcta attttaattt cacattgaat aattcttcga     5160

tgttagcgga cgtgacagat aaactcaata atttaataaa cataagaata aacttgcaat     5220

ctgactatcg ggatatcaat aaccgattct tagaagccgc caaagaacta aaagaagtag     5280

aagataaatt aaagcgatat cacgatcttg tacataaaaa ttatatttct aaagaaacta     5340

tgtccgattt aaggattaaa agagattatt ggcacgatgt ttatctattc tacaaaaaat     5400

taaaaaatga taaagatcgt gatatatcaa aacagttaaa ggaaatcgac gagtttgtcg     5460

aaaaacagag aaaactctct gacatcatcg aaaatggttt cgagcagctt tcaataaaat     5520

cccctatcgc cggtaatatc agttcattag atttaatttt aggacaacgg ttaaaacctg     5580

gcgataaaat agctattgtt gatgatttat caaactttta ttttgaatca gaaatcaacg     5640

aatactattt aaacaaaata acataccatt catctgccag tttaatctat aacaatggaa     5700

aaataccttt actggtgaaa ttaatttcat ctgaagtgaa taatggaaca ttcaaagtca     5760

gatttgaatt ggaagataaa aaaacaatta acttcaaacg ggggcaatcc gttgatgtca     5820

tgattaaact tgatgaagat cggaaaatat tctccgtacc ttcatctatg gtgttttcta     5880

ttgacaataa aaactatgtg tttgtttacc atccagataa aaaaattgcc cgacgtattc     5940

aagtttccac cggccaagat aatggttctg atattgagat caaaagtggt gttactgaag     6000

gacaaacgct cgttagcttt ggtaagaata aattagttaa taatgataca gtaaggattg     6060

aataaaatga ttagcatgat gaatatctgt aaatcatata aaacgaaatt cattcaaaca     6120

aatgttttga atgacataaa tctcaatata gataaaggcg aatttatttc tataatggga     6180

gcgtcagggt cagggaaatc aacattactg aatgtcattg gcatgtttga aacaatcgat     6240

agcggtcaat taacactgaa caatagcaat attttaacaa tgaaatattc tgaaaaaata     6300

tcattcagac gagaatttat tggttatatt tttcaatcat tcaatctgtt acccaatttg     6360

acggtatttg aaaacataga gttaccgtta aaatacagag gttttcctaa aaaacagcgg     6420

aaaagcaaag tatttgatgc aataaatagt tttggattag aaaatcggga aaatcataaa     6480

ccgatacaat tatctggtgg acaacagcaa cgtgttgcta tcgccagagc aatgatagca     6540

aaccccacca ttttattagc tgatgaacca acggggaacc tggatagcgt aaatggtcag     6600

aatattttat cttcacttaa ggagctgaat gaaaacggta cgacgattgt tatggtaacc     6660

cattccgtag aagcggcagc gttttctgac agaatattaa cgatgaggga tggccacttg     6720

cttgtttgag cataaataac ataataaagg agttctttat ggacacaata atccccataa     6780

aatatttaga ttcagacgaa tcatcgattc ttaagaaatc atctaaaatt aactacaggc     6840

aattagcttg cagaattatc ggtgaaatct ccgccgaaaa aatattagat gatgatgaac     6900

tggctttata taataaagaa atcagtatac atttcagccc tgaaattatt aatgctaata     6960

aattagttgt ggttgtgaaa gccaccaggc tttgcaattt aagatgcact tattgtcact     7020

cctgggcaga aggaaaagga aataccttaa cattcttcaa tttaatgcgt tccattcacc     7080

gtttcttatc cctaccgaat atcaagcgat ttgaattcgt ctggcatggc ggcgaagtaa     7140

cgttgttgaa tgttaattac tttaagaaac tcatctggtt acaggaacaa tttaaaaaac     7200

cggatcaagt tatcaccaat tcggtacaga caaatgccgt caatattcct gaagattggt     7260

tagtgttcct caaaggtatt ggaatggggg taggaataag cgttgatggt attccggaaa     7320

tacacgatag caggagatta gattacagag gaaggccaac atcccataaa gtcgcggcaa     7380

gtatgaaaaa gttaagaagt tatggcatac cttacggtgc gcttatcgtc gtcgaccgcg     7440

atgtttatga atcaaatata gaaaaaatgc tctcttattt ttacgaaatc ggtttaacgg     7500

atattgaatt tctgaatatt gtcccagata accgatgcca gccgggtgat gatcctggag     7560

gaagttatat aacttaccat aactatatta atttcctttc taaggttttc cgtgtctggt     7620

ggaatggtta tcaaggcaaa atcaatattc gcttgtttga cggatttatt gacagtatca     7680

aatcgtccca aaagaaaatg tcagattgtt attgggcggg taactgttct caggaaataa     7740

tcacattaga acctaatggt acggtatcag catgtgataa atatgttggt gctgaaggga     7800

ataattatgg ttcgattatt gataatgatc ttgggaattt actatctaaa tcaaatacaa     7860

ataaggatca tcttaaagag gaaatggaat cttatgaaaa aatgcatcaa tgtaaatggt     7920

ttcatttgtg taatggtgga tgcccacacg atcgagtgac caacaggaag cacaatccaa     7980

attatgatgg ttcatgttgt ggaaccggcg gtttgttgga gacaataaaa caaaccatcg     8040

cggcgtaaat tctaactagt tcctgattaa ttaaccaccc tgcaagggcc cggaaccgca     8100

aggttccggg cccttcgcct tttcgtcgac g                                    8131


<210>  87
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Peptide Derivative - Darobactin 39


<220>
<221>  CDS
<222>  (1)..(21)
<223>  Propetide core sequence - Darobactin 39

<400>  87
tgg aac tgg act cgg cgg tgg                                             21
Trp Asn Trp Thr Arg Arg Trp                                               
1               5                                                         


<210>  88
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  88

Trp Asn Trp Thr Arg Arg Trp 
1               5           


<210>  89
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer - pr2dar39_rv containing mutations of D39

<400>  89
aaatttcctg ccaccgccga gtccagttcc a                                      31


