                         SEQUENCE LISTING

<110>  Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer
       Gesundheit und Umwelt (GmbH)
 
<120>  CRISPR/Cas9-mediated means and methods for cell reprogramming

<130>  HEL17072PCT

<150>  LU102570
<151>  2021-02-17

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  2109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct N-dCas9_N-Intein


<220>
<221>  CDS
<222>  (1)..(2109)

<400>  1
atg gga cct aag aaa aag agg aag gtg gcg gcc gct gac tac aag gat         48
Met Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Asp Tyr Lys Asp           
1               5                   10                  15                

gac gac gat aaa ggt atc cac gga gtc cca gca gcc gac aag aag tac         96
Asp Asp Asp Lys Gly Ile His Gly Val Pro Ala Ala Asp Lys Lys Tyr           
            20                  25                  30                    

agc atc ggc ctg gcc atc ggc acc aac tct gtg ggc tgg gcc gtg atc        144
Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile           
        35                  40                  45                        

acc gac gag tac aag gtg ccc agc aag aaa ttc aag gtg ctg ggc aac        192
Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn           
    50                  55                  60                            

acc gac cgg cac agc atc aag aag aac ctg atc gga gcc ctg ctg ttc        240
Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe           
65                  70                  75                  80            

gac agc ggc gaa aca gcc gag gcc acc cgg ctg aag aga acc gcc aga        288
Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg           
                85                  90                  95                

aga aga tac acc aga cgg aag aac cgg atc tgc tat ctg caa gag atc        336
Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile           
            100                 105                 110                   

ttc agc aac gag atg gcc aag gtg gac gac agc ttc ttc cac aga ctg        384
Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu           
        115                 120                 125                       

gaa gag tcc ttc ctg gtg gaa gag gat aag aag cac gag cgg cac ccc        432
Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro           
    130                 135                 140                           

atc ttc ggc aac atc gtg gac gag gtg gcc tac cac gag aag tac ccc        480
Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro           
145                 150                 155                 160           

acc atc tac cac ctg aga aag aaa ctg gtg gac agc acc gac aag gcc        528
Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala           
                165                 170                 175               

gac ctg cgg ctg atc tat ctg gcc ctg gcc cac atg atc aag ttc cgg        576
Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg           
            180                 185                 190                   

ggc cac ttc ctg atc gag ggc gac ctg aac ccc gac aac agc gac gtg        624
Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val           
        195                 200                 205                       

gac aag ctg ttc atc cag ctg gtg cag acc tac aac cag ctg ttc gag        672
Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu           
    210                 215                 220                           

gaa aac ccc atc aac gcc agc ggc gtg gac gcc aag gcc atc ctg tct        720
Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser           
225                 230                 235                 240           

gcc aga ctg agc aag agc aga cgg ctg gaa aat ctg atc gcc cag ctg        768
Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu           
                245                 250                 255               

ccc ggc gag aag aag aat ggc ctg ttc ggc aac ctg att gcc ctg agc        816
Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser           
            260                 265                 270                   

ctg ggc ctg acc ccc aac ttc aag agc aac ttc gac ctg gcc gag gat        864
Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp           
        275                 280                 285                       

gcc aaa ctg cag ctg agc aag gac acc tac gac gac gac ctg gac aac        912
Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn           
    290                 295                 300                           

ctg ctg gcc cag atc ggc gac cag tac gcc gac ctg ttt ctg gcc gcc        960
Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala           
305                 310                 315                 320           

aag aac ctg tcc gac gcc atc ctg ctg agc gac atc ctg aga gtg aac       1008
Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn           
                325                 330                 335               

acc gag atc acc aag gcc ccc ctg agc gcc tct atg atc aag aga tac       1056
Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr           
            340                 345                 350                   

gac gag cac cac cag gac ctg acc ctg ctg aaa gct ctc gtg cgg cag       1104
Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln           
        355                 360                 365                       

cag ctg cct gag aag tac aaa gag att ttc ttc gac cag agc aag aac       1152
Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn           
    370                 375                 380                           

ggc tac gcc ggc tac att gac ggc gga gcc agc cag gaa gag ttc tac       1200
Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr           
385                 390                 395                 400           

aag ttc atc aag ccc atc ctg gaa aag atg gac ggc acc gag gaa ctg       1248
Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu           
                405                 410                 415               

ctc gtg aag ctg aac aga gag gac ctg ctg cgg aag cag cgg acc ttc       1296
Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe           
            420                 425                 430                   

gac aac ggc agc atc ccc cac cag atc cac ctg gga gag ctg cac gcc       1344
Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala           
        435                 440                 445                       

att ctg cgg cgg cag gaa gat ttt tac cca ttc ctg aag gac aac cgg       1392
Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg           
    450                 455                 460                           

gaa aag atc gag aag atc ctg acc ttc cgc atc ccc tac tac gtg ggc       1440
Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly           
465                 470                 475                 480           

cct ctg gcc agg gga aac agc aga ttc gcc tgg atg acc aga aag agc       1488
Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser           
                485                 490                 495               

gag gaa acc atc acc ccc tgg aac ttc gag gaa gtg gtg gac aag ggc       1536
Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly           
            500                 505                 510                   

gct tcc gcc cag agc ttc atc gag cgg atg acc aac ttc gat aag aac       1584
Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn           
        515                 520                 525                       

ctg ccc aac gag aag gtg ctg ccc aag cac agc ctg ctg tac gag tac       1632
Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr           
    530                 535                 540                           

ttc acc gtg tat aac gag ctg acc aaa gtg aaa tac gtg acc gag gga       1680
Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly           
545                 550                 555                 560           

atg aga aag ccc gcc ttc ctg agc ggc gag cag aaa aag gcc atc gtg       1728
Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val           
                565                 570                 575               

gac ctg ctg ttc aag acc aac cgg aaa gtg acc gtg aag cag ctg aaa       1776
Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys           
            580                 585                 590                   

gag gac tac ttc aag aaa atc gag tgc ctg tcc tac gag acc gaa atc       1824
Glu Asp Tyr Phe Lys Lys Ile Glu Cys Leu Ser Tyr Glu Thr Glu Ile           
        595                 600                 605                       

ctg acc gtg gag tat ggg ctg ctg ccc atc ggc aag att gtg gag aag       1872
Leu Thr Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys           
    610                 615                 620                           

cgg att gaa tgc acc gtg tat agc gtg gac aac aac ggc aac atc tac       1920
Arg Ile Glu Cys Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr           
625                 630                 635                 640           

acc cag ccc gtg gct cag tgg cac gac agg ggc gag cag gag gtg ttt       1968
Thr Gln Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe           
                645                 650                 655               

gag tat tgt ctg gag gac ggc agc ctg att aga gcc acc aaa gac cac       2016
Glu Tyr Cys Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His           
            660                 665                 670                   

aag ttc atg acc gtg gac ggg cag atg ctg ccc att gac gag att ttt       2064
Lys Phe Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe           
        675                 680                 685                       

gag cgg gaa ctt gac ctg atg cgg gtg gac aac ctg ccc aac tga           2109
Glu Arg Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn                   
    690                 695                 700                           


<210>  2
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Asp Tyr Lys Asp 
1               5                   10                  15      


Asp Asp Asp Lys Gly Ile His Gly Val Pro Ala Ala Asp Lys Lys Tyr 
            20                  25                  30          


Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile 
        35                  40                  45              


Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn 
    50                  55                  60                  


Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe 
65                  70                  75                  80  


Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg 
                85                  90                  95      


Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile 
            100                 105                 110         


Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu 
        115                 120                 125             


Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro 
    130                 135                 140                 


Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro 
145                 150                 155                 160 


Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala 
                165                 170                 175     


Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg 
            180                 185                 190         


Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val 
        195                 200                 205             


Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu 
    210                 215                 220                 


Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser 
225                 230                 235                 240 


Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu 
                245                 250                 255     


Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser 
            260                 265                 270         


Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp 
        275                 280                 285             


Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn 
    290                 295                 300                 


Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala 
305                 310                 315                 320 


Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn 
                325                 330                 335     


Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr 
            340                 345                 350         


Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln 
        355                 360                 365             


Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn 
    370                 375                 380                 


Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr 
385                 390                 395                 400 


Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu 
                405                 410                 415     


Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe 
            420                 425                 430         


Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala 
        435                 440                 445             


Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg 
    450                 455                 460                 


Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly 
465                 470                 475                 480 


Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser 
                485                 490                 495     


Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly 
            500                 505                 510         


Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn 
        515                 520                 525             


Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr 
    530                 535                 540                 


Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly 
545                 550                 555                 560 


Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val 
                565                 570                 575     


Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys 
            580                 585                 590         


Glu Asp Tyr Phe Lys Lys Ile Glu Cys Leu Ser Tyr Glu Thr Glu Ile 
        595                 600                 605             


Leu Thr Val Glu Tyr Gly Leu Leu Pro Ile Gly Lys Ile Val Glu Lys 
    610                 615                 620                 


Arg Ile Glu Cys Thr Val Tyr Ser Val Asp Asn Asn Gly Asn Ile Tyr 
625                 630                 635                 640 


Thr Gln Pro Val Ala Gln Trp His Asp Arg Gly Glu Gln Glu Val Phe 
                645                 650                 655     


Glu Tyr Cys Leu Glu Asp Gly Ser Leu Ile Arg Ala Thr Lys Asp His 
            660                 665                 670         


Lys Phe Met Thr Val Asp Gly Gln Met Leu Pro Ile Asp Glu Ile Phe 
        675                 680                 685             


Glu Arg Glu Leu Asp Leu Met Arg Val Asp Asn Leu Pro Asn 
    690                 695                 700         


<210>  3
<211>  2790
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct C-dCas9_VP64_C-Intein


<220>
<221>  CDS
<222>  (1)..(2790)

<400>  3
atg ata aag att gcc acc aga aag tat ctg ggc aag cag aac gtg tat         48
Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr           
1               5                   10                  15                

gac atc ggc gtg gag aga gac cac aac ttc gcc ctg aag aac ggc ttc         96
Asp Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe           
            20                  25                  30                    

atc gcc agc aac tgc ttc gac tcc gtg gaa atc tcc ggc gtg gaa gat        144
Ile Ala Ser Asn Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp           
        35                  40                  45                        

cgg ttc aac gcc tcc ctg ggc aca tac cac gat ctg ctg aaa att atc        192
Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile           
    50                  55                  60                            

aag gac aag gac ttc ctg gac aat gag gaa aac gag gac att ctg gaa        240
Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu           
65                  70                  75                  80            

gat atc gtg ctg acc ctg aca ctg ttt gag gac aga gag atg atc gag        288
Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu           
                85                  90                  95                

gaa cgg ctg aaa acc tat gcc cac ctg ttc gac gac aaa gtg atg aag        336
Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys           
            100                 105                 110                   

cag ctg aag cgg cgg aga tac acc ggc tgg ggc agg ctg agc cgg aag        384
Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys           
        115                 120                 125                       

ctg atc aac ggc atc cgg gac aag cag tcc ggc aag aca atc ctg gat        432
Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp           
    130                 135                 140                           

ttc ctg aag tcc gac ggc ttc gcc aac aga aac ttc atg cag ctg atc        480
Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile           
145                 150                 155                 160           

cac gac gac agc ctg acc ttt aaa gag gac atc cag aaa gcc cag gtg        528
His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val           
                165                 170                 175               

tcc ggc cag ggc gat agc ctg cac gag cac att gcc aat ctg gcc ggc        576
Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly           
            180                 185                 190                   

agc ccc gcc att aag aag ggc atc ctg cag aca gtg aag gtg gtg gac        624
Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp           
        195                 200                 205                       

gag ctc gtg aaa gtg atg ggc cgg cac aag ccc gag aac atc gtg atc        672
Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile           
    210                 215                 220                           

gaa atg gcc aga gag aac cag acc acc cag aag gga cag aag aac agc        720
Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser           
225                 230                 235                 240           

cgc gag aga atg aag cgg atc gaa gag ggc atc aaa gag ctg ggc agc        768
Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser           
                245                 250                 255               

cag atc ctg aaa gaa cac ccc gtg gaa aac acc cag ctg cag aac gag        816
Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu           
            260                 265                 270                   

aag ctg tac ctg tac tac ctg cag aat ggg cgg gat atg tac gtg gac        864
Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp           
        275                 280                 285                       

cag gaa ctg gac atc aac cgg ctg tcc gac tac gat gtg gac cat atc        912
Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp His Ile           
    290                 295                 300                           

gtg cct cag agc ttt ctg aag gac gac tcc atc gac aac aag gtg ctg        960
Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu           
305                 310                 315                 320           

acc aga agc gac aag gcc cgg ggc aag agc gac aac gtg ccc tcc gaa       1008
Thr Arg Ser Asp Lys Ala Arg Gly Lys Ser Asp Asn Val Pro Ser Glu           
                325                 330                 335               

gag gtc gtg aag aag atg aag aac tac tgg cgg cag ctg ctg aac gcc       1056
Glu Val Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala           
            340                 345                 350                   

aag ctg att acc cag aga aag ttc gac aat ctg acc aag gcc gag aga       1104
Lys Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg           
        355                 360                 365                       

ggc ggc ctg agc gaa ctg gat aag gcc ggc ttc atc aag aga cag ctg       1152
Gly Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu           
    370                 375                 380                           

gtg gaa acc cgg cag atc aca aag cac gtg gca cag atc ctg gac tcc       1200
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser           
385                 390                 395                 400           

cgg atg aac act aag tac gac gag aat gac aag ctg atc cgg gaa gtg       1248
Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val           
                405                 410                 415               

aaa gtg atc acc ctg aag tcc aag ctg gtg tcc gat ttc cgg aag gat       1296
Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp           
            420                 425                 430                   

ttc cag ttt tac aaa gtg cgc gag atc aac aac tac cac cac gcc cac       1344
Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His           
        435                 440                 445                       

gac gcc tac ctg aac gcc gtc gtg gga acc gcc ctg atc aaa aag tac       1392
Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr           
    450                 455                 460                           

cct aag ctg gaa agc gag ttc gtg tac ggc gac tac aag gtg tac gac       1440
Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp           
465                 470                 475                 480           

gtg cgg aag atg atc gcc aag agc gag cag gaa atc ggc aag gct acc       1488
Val Arg Lys Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr           
                485                 490                 495               

gcc aag tac ttc ttc tac agc aac atc atg aac ttt ttc aag acc gag       1536
Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu           
            500                 505                 510                   

att acc ctg gcc aac ggc gag atc cgg aag cgg cct ctg atc gag aca       1584
Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr           
        515                 520                 525                       

aac ggc gaa acc ggg gag atc gtg tgg gat aag ggc cgg gat ttt gcc       1632
Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala           
    530                 535                 540                           

acc gtg cgg aaa gtg ctg agc atg ccc caa gtg aat atc gtg aaa aag       1680
Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys           
545                 550                 555                 560           

acc gag gtg cag aca ggc ggc ttc agc aaa gag tct atc ctg ccc aag       1728
Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys           
                565                 570                 575               

agg aac agc gat aag ctg atc gcc aga aag aag gac tgg gac cct aag       1776
Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys           
            580                 585                 590                   

aag tac ggc ggc ttc gac agc ccc acc gtg gcc tat tct gtg ctg gtg       1824
Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val           
        595                 600                 605                       

gtg gcc aaa gtg gaa aag ggc aag tcc aag aaa ctg aag agt gtg aaa       1872
Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys           
    610                 615                 620                           

gag ctg ctg ggg atc acc atc atg gaa aga agc agc ttc gag aag aat       1920
Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn           
625                 630                 635                 640           

ccc atc gac ttt ctg gaa gcc aag ggc tac aaa gaa gtg aaa aag gac       1968
Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp           
                645                 650                 655               

ctg atc atc aag ctg cct aag tac tcc ctg ttc gag ctg gaa aac ggc       2016
Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly           
            660                 665                 670                   

cgg aag aga atg ctg gcc tct gcc ggc gaa ctg cag aag gga aac gaa       2064
Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu           
        675                 680                 685                       

ctg gcc ctg ccc tcc aaa tat gtg aac ttc ctg tac ctg gcc agc cac       2112
Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His           
    690                 695                 700                           

tat gag aag ctg aag ggc tcc ccc gag gat aat gag cag aaa cag ctg       2160
Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu           
705                 710                 715                 720           

ttt gtg gaa cag cac aag cac tac ctg gac gag atc atc gag cag atc       2208
Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile           
                725                 730                 735               

agc gag ttc tcc aag aga gtg atc ctg gcc gac gct aat ctg gac aaa       2256
Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys           
            740                 745                 750                   

gtg ctg tcc gcc tac aac aag cac cgg gat aag ccc atc aga gag cag       2304
Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln           
        755                 760                 765                       

gcc gag aat atc atc cac ctg ttt acc ctg acc aat ctg gga gcc cct       2352
Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro           
    770                 775                 780                           

gcc gcc ttc aag tac ttt gac acc acc atc gac cgg aag agg tac acc       2400
Ala Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr           
785                 790                 795                 800           

agc acc aaa gag gtg ctg gac gcc acc ctg atc cac cag agc atc acc       2448
Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr           
                805                 810                 815               

ggc ctg tac gag aca cgg atc gac ctg tct cag ctg gga ggc gac gcc       2496
Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ala           
            820                 825                 830                   

tat ccc tat gac gtg ccc gat tat gcc agc ctg ggc agc ggc tca ccc       2544
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Gly Ser Gly Ser Pro           
        835                 840                 845                       

aag aag aag agg aag gtg gct gca gga ggc ggt gga agc ggg cgc gcc       2592
Lys Lys Lys Arg Lys Val Ala Ala Gly Gly Gly Gly Ser Gly Arg Ala           
    850                 855                 860                           

gac gca ttg gac gat ttt gat ctg gat atg ctg gga agt gac gcc ctc       2640
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu           
865                 870                 875                 880           

gat gat ttt gac ctt gac atg ctt ggt tcg gat gcc ctt gat gac ttt       2688
Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe           
                885                 890                 895               

gac ctc gac atg ctc ggc agt gac gcc ctt gat gat ttc gac ctg gac       2736
Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp           
            900                 905                 910                   

atg ctg att aac tct aga agt tcc gga tct ccg aaa aag aaa cgc aaa       2784
Met Leu Ile Asn Ser Arg Ser Ser Gly Ser Pro Lys Lys Lys Arg Lys           
        915                 920                 925                       

gtt tga                                                               2790
Val                                                                       
                                                                          


<210>  4
<211>  929
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Ile Lys Ile Ala Thr Arg Lys Tyr Leu Gly Lys Gln Asn Val Tyr 
1               5                   10                  15      


Asp Ile Gly Val Glu Arg Asp His Asn Phe Ala Leu Lys Asn Gly Phe 
            20                  25                  30          


Ile Ala Ser Asn Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp 
        35                  40                  45              


Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile 
    50                  55                  60                  


Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu 
65                  70                  75                  80  


Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu 
                85                  90                  95      


Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys 
            100                 105                 110         


Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys 
        115                 120                 125             


Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp 
    130                 135                 140                 


Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile 
145                 150                 155                 160 


His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val 
                165                 170                 175     


Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly 
            180                 185                 190         


Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp 
        195                 200                 205             


Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile 
    210                 215                 220                 


Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser 
225                 230                 235                 240 


Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser 
                245                 250                 255     


Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu 
            260                 265                 270         


Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp 
        275                 280                 285             


Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp His Ile 
    290                 295                 300                 


Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu 
305                 310                 315                 320 


Thr Arg Ser Asp Lys Ala Arg Gly Lys Ser Asp Asn Val Pro Ser Glu 
                325                 330                 335     


Glu Val Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala 
            340                 345                 350         


Lys Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
        355                 360                 365             


Gly Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu 
    370                 375                 380                 


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser 
385                 390                 395                 400 


Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val 
                405                 410                 415     


Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp 
            420                 425                 430         


Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His 
        435                 440                 445             


Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr 
    450                 455                 460                 


Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp 
465                 470                 475                 480 


Val Arg Lys Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr 
                485                 490                 495     


Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu 
            500                 505                 510         


Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr 
        515                 520                 525             


Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala 
    530                 535                 540                 


Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys 
545                 550                 555                 560 


Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys 
                565                 570                 575     


Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys 
            580                 585                 590         


Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val 
        595                 600                 605             


Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys 
    610                 615                 620                 


Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn 
625                 630                 635                 640 


Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp 
                645                 650                 655     


Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly 
            660                 665                 670         


Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu 
        675                 680                 685             


Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His 
    690                 695                 700                 


Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu 
705                 710                 715                 720 


Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile 
                725                 730                 735     


Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys 
            740                 745                 750         


Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln 
        755                 760                 765             


Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro 
    770                 775                 780                 


Ala Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr 
785                 790                 795                 800 


Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr 
                805                 810                 815     


Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ala 
            820                 825                 830         


Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Gly Ser Gly Ser Pro 
        835                 840                 845             


Lys Lys Lys Arg Lys Val Ala Ala Gly Gly Gly Gly Ser Gly Arg Ala 
    850                 855                 860                 


Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu 
865                 870                 875                 880 


Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe 
                885                 890                 895     


Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp 
            900                 905                 910         


Met Leu Ile Asn Ser Arg Ser Ser Gly Ser Pro Lys Lys Lys Arg Lys 
        915                 920                 925             


Val 
    


<210>  5
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct CBh_flexed-GFP

<400>  5
ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga       60

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      120

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      180

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      240

attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc      300

ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg      360

gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg      420

cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg      480

aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg      540

acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg      600

actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa      660

ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg      720

gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct      780

gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta      840

gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc      900

tggttattgt gctgtctcat cattttggca aagaattgga tccccataac ttcgtataaa      960

gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc gacccgaatt     1020

gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa gttatactag     1080

ggatcccccc tcgagtttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg     1140

gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg     1200

gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg     1260

ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg     1320

aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg     1380

tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc     1440

agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag     1500

ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac     1560

ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag     1620

gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac     1680

ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg     1740

tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc     1800

tcgcccttgc tcaccatggt ggcggtaccg aattctagta taacttcgta taggatactt     1860

tatacgaagt tatcattggg attcttccta ttttgatcca agcatcacca tcgaccctct     1920

agtccagatc tcaccatcga cccataactt cgtatagcat acattatacg aagttatgtc     1980

cctcgaagag gttcgaattc gataataaag gaaatttatt ttcattgcaa tagtgtgttg     2040

gttttttgtg tg                                                         2052


<210>  6
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein construct CBh_flexed-GFP

<400>  6

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  7
<211>  5793
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct HA_SpCas9(D10A,H840A)_VPR


<220>
<221>  CDS
<222>  (1)..(5793)

<400>  7
atg tac cca tac gat gtt cca gat tac gct tcg ccg aag aaa aag cgc         48
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg           
1               5                   10                  15                

aag gtc gaa gcg tcc gac aag aag tac agc atc ggc ctg gcc atc ggc         96
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly           
            20                  25                  30                    

acc aac tct gtg ggc tgg gcc gtg atc acc gac gag tac aag gtg ccc        144
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro           
        35                  40                  45                        

agc aag aaa ttc aag gtg ctg ggc aac acc gac cgg cac agc atc aag        192
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys           
    50                  55                  60                            

aag aac ctg atc gga gcc ctg ctg ttc gac agc ggc gaa aca gcc gag        240
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu           
65                  70                  75                  80            

gcc acc cgg ctg aag aga acc gcc aga aga aga tac acc aga cgg aag        288
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys           
                85                  90                  95                

aac cgg atc tgc tat ctg caa gag atc ttc agc aac gag atg gcc aag        336
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys           
            100                 105                 110                   

gtg gac gac agc ttc ttc cac aga ctg gaa gag tcc ttc ctg gtg gaa        384
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu           
        115                 120                 125                       

gag gat aag aag cac gag cgg cac ccc atc ttc ggc aac atc gtg gac        432
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp           
    130                 135                 140                           

gag gtg gcc tac cac gag aag tac ccc acc atc tac cac ctg aga aag        480
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys           
145                 150                 155                 160           

aaa ctg gtg gac agc acc gac aag gcc gac ctg cgg ctg atc tat ctg        528
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu           
                165                 170                 175               

gcc ctg gcc cac atg atc aag ttc cgg ggc cac ttc ctg atc gag ggc        576
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly           
            180                 185                 190                   

gac ctg aac ccc gac aac agc gac gtg gac aag ctg ttc atc cag ctg        624
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu           
        195                 200                 205                       

gtg cag acc tac aac cag ctg ttc gag gaa aac ccc atc aac gcc agc        672
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser           
    210                 215                 220                           

ggc gtg gac gcc aag gcc atc ctg tct gcc aga ctg agc aag agc aga        720
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg           
225                 230                 235                 240           

cgg ctg gaa aat ctg atc gcc cag ctg ccc ggc gag aag aag aat ggc        768
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly           
                245                 250                 255               

ctg ttc ggc aac ctg att gcc ctg agc ctg ggc ctg acc ccc aac ttc        816
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe           
            260                 265                 270                   

aag agc aac ttc gac ctg gcc gag gat gcc aaa ctg cag ctg agc aag        864
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys           
        275                 280                 285                       

gac acc tac gac gac gac ctg gac aac ctg ctg gcc cag atc ggc gac        912
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp           
    290                 295                 300                           

cag tac gcc gac ctg ttt ctg gcc gcc aag aac ctg tcc gac gcc atc        960
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile           
305                 310                 315                 320           

ctg ctg agc gac atc ctg aga gtg aac acc gag atc acc aag gcc ccc       1008
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro           
                325                 330                 335               

ctg agc gcc tct atg atc aag aga tac gac gag cac cac cag gac ctg       1056
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu           
            340                 345                 350                   

acc ctg ctg aaa gct ctc gtg cgg cag cag ctg cct gag aag tac aaa       1104
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys           
        355                 360                 365                       

gag att ttc ttc gac cag agc aag aac ggc tac gcc ggc tac att gac       1152
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp           
    370                 375                 380                           

ggc gga gcc agc cag gaa gag ttc tac aag ttc atc aag ccc atc ctg       1200
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu           
385                 390                 395                 400           

gaa aag atg gac ggc acc gag gaa ctg ctc gtg aag ctg aac aga gag       1248
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu           
                405                 410                 415               

gac ctg ctg cgg aag cag cgg acc ttc gac aac ggc agc atc ccc cac       1296
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His           
            420                 425                 430                   

cag atc cac ctg gga gag ctg cac gcc att ctg cgg cgg cag gaa gat       1344
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp           
        435                 440                 445                       

ttt tac cca ttc ctg aag gac aac cgg gaa aag atc gag aag atc ctg       1392
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu           
    450                 455                 460                           

acc ttc cgc atc ccc tac tac gtg ggc cct ctg gcc agg gga aac agc       1440
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser           
465                 470                 475                 480           

aga ttc gcc tgg atg acc aga aag agc gag gaa acc atc acc ccc tgg       1488
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp           
                485                 490                 495               

aac ttc gag gaa gtg gtg gac aag ggc gct tcc gcc cag agc ttc atc       1536
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile           
            500                 505                 510                   

gag cgg atg acc aac ttc gat aag aac ctg ccc aac gag aag gtg ctg       1584
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu           
        515                 520                 525                       

ccc aag cac agc ctg ctg tac gag tac ttc acc gtg tat aac gag ctg       1632
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu           
    530                 535                 540                           

acc aaa gtg aaa tac gtg acc gag gga atg aga aag ccc gcc ttc ctg       1680
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu           
545                 550                 555                 560           

agc ggc gag cag aaa aag gcc atc gtg gac ctg ctg ttc aag acc aac       1728
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn           
                565                 570                 575               

cgg aaa gtg acc gtg aag cag ctg aaa gag gac tac ttc aag aaa atc       1776
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile           
            580                 585                 590                   

gag tgc ttc gac tcc gtg gaa atc tcc ggc gtg gaa gat cgg ttc aac       1824
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn           
        595                 600                 605                       

gcc tcc ctg ggc aca tac cac gat ctg ctg aaa att atc aag gac aag       1872
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys           
    610                 615                 620                           

gac ttc ctg gac aat gag gaa aac gag gac att ctg gaa gat atc gtg       1920
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val           
625                 630                 635                 640           

ctg acc ctg aca ctg ttt gag gac aga gag atg atc gag gaa cgg ctg       1968
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu           
                645                 650                 655               

aaa acc tat gcc cac ctg ttc gac gac aaa gtg atg aag cag ctg aag       2016
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys           
            660                 665                 670                   

cgg cgg aga tac acc ggc tgg ggc agg ctg agc cgg aag ctg atc aac       2064
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn           
        675                 680                 685                       

ggc atc cgg gac aag cag tcc ggc aag aca atc ctg gat ttc ctg aag       2112
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys           
    690                 695                 700                           

tcc gac ggc ttc gcc aac aga aac ttc atg cag ctg atc cac gac gac       2160
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp           
705                 710                 715                 720           

agc ctg acc ttt aaa gag gac atc cag aaa gcc cag gtg tcc ggc cag       2208
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln           
                725                 730                 735               

ggc gat agc ctg cac gag cac att gcc aat ctg gcc ggc agc ccc gcc       2256
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala           
            740                 745                 750                   

att aag aag ggc atc ctg cag aca gtg aag gtg gtg gac gag ctc gtg       2304
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val           
        755                 760                 765                       

aaa gtg atg ggc cgg cac aag ccc gag aac atc gtg atc gaa atg gcc       2352
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala           
    770                 775                 780                           

aga gag aac cag acc acc cag aag gga cag aag aac agc cgc gag aga       2400
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg           
785                 790                 795                 800           

atg aag cgg atc gaa gag ggc atc aaa gag ctg ggc agc cag atc ctg       2448
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu           
                805                 810                 815               

aaa gaa cac ccc gtg gaa aac acc cag ctg cag aac gag aag ctg tac       2496
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr           
            820                 825                 830                   

ctg tac tac ctg cag aat ggg cgg gat atg tac gtg gac cag gaa ctg       2544
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu           
        835                 840                 845                       

gac atc aac cgg ctg tcc gac tac gat gtg gac gcc atc gtg cct cag       2592
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln           
    850                 855                 860                           

agc ttt ctg aag gac gac tcc atc gac aac aag gtg ctg acc aga agc       2640
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser           
865                 870                 875                 880           

gac aag aac cgg ggc aag agc gac aac gtg ccc tcc gaa gag gtc gtg       2688
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val           
                885                 890                 895               

aag aag atg aag aac tac tgg cgg cag ctg ctg aac gcc aag ctg att       2736
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile           
            900                 905                 910                   

acc cag aga aag ttc gac aat ctg acc aag gcc gag aga ggc ggc ctg       2784
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu           
        915                 920                 925                       

agc gaa ctg gat aag gcc ggc ttc atc aag aga cag ctg gtg gaa acc       2832
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr           
    930                 935                 940                           

cgg cag atc aca aag cac gtg gca cag atc ctg gac tcc cgg atg aac       2880
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn           
945                 950                 955                 960           

act aag tac gac gag aat gac aag ctg atc cgg gaa gtg aaa gtg atc       2928
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile           
                965                 970                 975               

acc ctg aag tcc aag ctg gtg tcc gat ttc cgg aag gat ttc cag ttt       2976
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe           
            980                 985                 990                   

tac aaa gtg cgc gag atc aac aac  tac cac cac gcc cac  gac gcc tac     3024
Tyr Lys Val Arg Glu Ile Asn Asn  Tyr His His Ala His  Asp Ala Tyr         
        995                 1000                 1005                     

ctg aac  gcc gtc gtg gga acc  gcc ctg atc aaa aag  tac cct aag        3069
Leu Asn  Ala Val Val Gly Thr  Ala Leu Ile Lys Lys  Tyr Pro Lys            
    1010                 1015                 1020                        

ctg gaa  agc gag ttc gtg tac  ggc gac tac aag gtg  tac gac gtg        3114
Leu Glu  Ser Glu Phe Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val            
    1025                 1030                 1035                        

cgg aag  atg atc gcc aag agc  gag cag gaa atc ggc  aag gct acc        3159
Arg Lys  Met Ile Ala Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr            
    1040                 1045                 1050                        

gcc aag  tac ttc ttc tac agc  aac atc atg aac ttt  ttc aag acc        3204
Ala Lys  Tyr Phe Phe Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr            
    1055                 1060                 1065                        

gag att  acc ctg gcc aac ggc  gag atc cgg aag cgg  cct ctg atc        3249
Glu Ile  Thr Leu Ala Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile            
    1070                 1075                 1080                        

gag aca  aac ggc gaa acc ggg  gag atc gtg tgg gat  aag ggc cgg        3294
Glu Thr  Asn Gly Glu Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg            
    1085                 1090                 1095                        

gat ttt  gcc acc gtg cgg aaa  gtg ctg agc atg ccc  caa gtg aat        3339
Asp Phe  Ala Thr Val Arg Lys  Val Leu Ser Met Pro  Gln Val Asn            
    1100                 1105                 1110                        

atc gtg  aaa aag acc gag gtg  cag aca ggc ggc ttc  agc aaa gag        3384
Ile Val  Lys Lys Thr Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu            
    1115                 1120                 1125                        

tct atc  ctg ccc aag agg aac  agc gat aag ctg atc  gcc aga aag        3429
Ser Ile  Leu Pro Lys Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys            
    1130                 1135                 1140                        

aag gac  tgg gac cct aag aag  tac ggc ggc ttc gac  agc ccc acc        3474
Lys Asp  Trp Asp Pro Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr            
    1145                 1150                 1155                        

gtg gcc  tat tct gtg ctg gtg  gtg gcc aaa gtg gaa  aag ggc aag        3519
Val Ala  Tyr Ser Val Leu Val  Val Ala Lys Val Glu  Lys Gly Lys            
    1160                 1165                 1170                        

tcc aag  aaa ctg aag agt gtg  aaa gag ctg ctg ggg  atc acc atc        3564
Ser Lys  Lys Leu Lys Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile            
    1175                 1180                 1185                        

atg gaa  aga agc agc ttc gag  aag aat ccc atc gac  ttt ctg gaa        3609
Met Glu  Arg Ser Ser Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu            
    1190                 1195                 1200                        

gcc aag  ggc tac aaa gaa gtg  aaa aag gac ctg atc  atc aag ctg        3654
Ala Lys  Gly Tyr Lys Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu            
    1205                 1210                 1215                        

cct aag  tac tcc ctg ttc gag  ctg gaa aac ggc cgg  aag aga atg        3699
Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met            
    1220                 1225                 1230                        

ctg gcc  tct gcc ggc gaa ctg  cag aag gga aac gaa  ctg gcc ctg        3744
Leu Ala  Ser Ala Gly Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu            
    1235                 1240                 1245                        

ccc tcc  aaa tat gtg aac ttc  ctg tac ctg gcc agc  cac tat gag        3789
Pro Ser  Lys Tyr Val Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu            
    1250                 1255                 1260                        

aag ctg  aag ggc tcc ccc gag  gat aat gag cag aaa  cag ctg ttt        3834
Lys Leu  Lys Gly Ser Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe            
    1265                 1270                 1275                        

gtg gaa  cag cac aag cac tac  ctg gac gag atc atc  gag cag atc        3879
Val Glu  Gln His Lys His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile            
    1280                 1285                 1290                        

agc gag  ttc tcc aag aga gtg  atc ctg gcc gac gct  aat ctg gac        3924
Ser Glu  Phe Ser Lys Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp            
    1295                 1300                 1305                        

aaa gtg  ctg tcc gcc tac aac  aag cac cgg gat aag  ccc atc aga        3969
Lys Val  Leu Ser Ala Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg            
    1310                 1315                 1320                        

gag cag  gcc gag aat atc atc  cac ctg ttt acc ctg  acc aat ctg        4014
Glu Gln  Ala Glu Asn Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu            
    1325                 1330                 1335                        

gga gcc  cct gcc gcc ttc aag  tac ttt gac acc acc  atc gac cgg        4059
Gly Ala  Pro Ala Ala Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg            
    1340                 1345                 1350                        

aag agg  tac acc agc acc aaa  gag gtg ctg gac gcc  acc ctg atc        4104
Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile            
    1355                 1360                 1365                        

cac cag  agc atc acc ggc ctg  tac gag aca cgg atc  gac ctg tct        4149
His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser            
    1370                 1375                 1380                        

cag ctg  gga ggc gac agc ccc  aag aag aag aga aag  gtg gag gcc        4194
Gln Leu  Gly Gly Asp Ser Pro  Lys Lys Lys Arg Lys  Val Glu Ala            
    1385                 1390                 1395                        

agc ggg  ccg gcc gga tcc gga  cgg gct gac gca ttg  gac gat ttt        4239
Ser Gly  Pro Ala Gly Ser Gly  Arg Ala Asp Ala Leu  Asp Asp Phe            
    1400                 1405                 1410                        

gat ctg  gat atg ctg gga agt  gac gcc ctc gat gat  ttt gac ctt        4284
Asp Leu  Asp Met Leu Gly Ser  Asp Ala Leu Asp Asp  Phe Asp Leu            
    1415                 1420                 1425                        

gac atg  ctt ggt tcg gat gcc  ctt gat gac ttt gac  ctc gac atg        4329
Asp Met  Leu Gly Ser Asp Ala  Leu Asp Asp Phe Asp  Leu Asp Met            
    1430                 1435                 1440                        

ctc ggc  agt gac gcc ctt gat  gat ttc gac ctg gac  atg ctg att        4374
Leu Gly  Ser Asp Ala Leu Asp  Asp Phe Asp Leu Asp  Met Leu Ile            
    1445                 1450                 1455                        

aac tct  aga agt tcc gga tct  ccg aaa aag aaa cgc  aaa gtt ggt        4419
Asn Ser  Arg Ser Ser Gly Ser  Pro Lys Lys Lys Arg  Lys Val Gly            
    1460                 1465                 1470                        

agc cag  tac ctg ccc gac acc  gac gac cgg cac cgg  atc gag gaa        4464
Ser Gln  Tyr Leu Pro Asp Thr  Asp Asp Arg His Arg  Ile Glu Glu            
    1475                 1480                 1485                        

aag cgg  aag cgg acc tac gag  aca ttc aag agc atc  atg aag aag        4509
Lys Arg  Lys Arg Thr Tyr Glu  Thr Phe Lys Ser Ile  Met Lys Lys            
    1490                 1495                 1500                        

tcc ccc  ttc agc ggc ccc acc  gac cct aga cct cca  cct aga aga        4554
Ser Pro  Phe Ser Gly Pro Thr  Asp Pro Arg Pro Pro  Pro Arg Arg            
    1505                 1510                 1515                        

atc gcc  gtg ccc agc aga tcc  agc gcc agc gtg cca  aaa cct gcc        4599
Ile Ala  Val Pro Ser Arg Ser  Ser Ala Ser Val Pro  Lys Pro Ala            
    1520                 1525                 1530                        

ccc cag  cct tac ccc ttc acc  agc agc ctg agc acc  atc aac tac        4644
Pro Gln  Pro Tyr Pro Phe Thr  Ser Ser Leu Ser Thr  Ile Asn Tyr            
    1535                 1540                 1545                        

gac gag  ttc cct acc atg gtg  ttc ccc agc ggc cag  atc tct cag        4689
Asp Glu  Phe Pro Thr Met Val  Phe Pro Ser Gly Gln  Ile Ser Gln            
    1550                 1555                 1560                        

gcc tct  gct ctg gct cca gcc  cct cct cag gtg ctg  cct cag gct        4734
Ala Ser  Ala Leu Ala Pro Ala  Pro Pro Gln Val Leu  Pro Gln Ala            
    1565                 1570                 1575                        

cct gct  cct gca cca gct cca  gcc atg gtg tct gca  ctg gct cag        4779
Pro Ala  Pro Ala Pro Ala Pro  Ala Met Val Ser Ala  Leu Ala Gln            
    1580                 1585                 1590                        

gca cca  gca ccc gtg cct gtg  ctg gct cct gga cct  cca cag gct        4824
Ala Pro  Ala Pro Val Pro Val  Leu Ala Pro Gly Pro  Pro Gln Ala            
    1595                 1600                 1605                        

gtg gct  cca cca gcc cct aaa  cct aca cag gcc ggc  gag ggc aca        4869
Val Ala  Pro Pro Ala Pro Lys  Pro Thr Gln Ala Gly  Glu Gly Thr            
    1610                 1615                 1620                        

ctg tct  gaa gct ctg ctg cag  ctg cag ttc gac gac  gag gat ctg        4914
Leu Ser  Glu Ala Leu Leu Gln  Leu Gln Phe Asp Asp  Glu Asp Leu            
    1625                 1630                 1635                        

gga gcc  ctg ctg gga aac agc  acc gat cct gcc gtg  ttc acc gac        4959
Gly Ala  Leu Leu Gly Asn Ser  Thr Asp Pro Ala Val  Phe Thr Asp            
    1640                 1645                 1650                        

ctg gcc  agc gtg gac aac agc  gag ttc cag cag ctg  ctg aac cag        5004
Leu Ala  Ser Val Asp Asn Ser  Glu Phe Gln Gln Leu  Leu Asn Gln            
    1655                 1660                 1665                        

ggc atc  cct gtg gcc cct cac  acc acc gag ccc atg  ctg atg gaa        5049
Gly Ile  Pro Val Ala Pro His  Thr Thr Glu Pro Met  Leu Met Glu            
    1670                 1675                 1680                        

tac ccc  gag gcc atc acc cgg  ctc gtg aca ggc gct  cag agg cct        5094
Tyr Pro  Glu Ala Ile Thr Arg  Leu Val Thr Gly Ala  Gln Arg Pro            
    1685                 1690                 1695                        

cct gat  cca gct cct gcc cct  ctg gga gca cca ggc  ctg cct aat        5139
Pro Asp  Pro Ala Pro Ala Pro  Leu Gly Ala Pro Gly  Leu Pro Asn            
    1700                 1705                 1710                        

gga ctg  ctg tct ggc gac gag  gac ttc agc tct atc  gcc gat atg        5184
Gly Leu  Leu Ser Gly Asp Glu  Asp Phe Ser Ser Ile  Ala Asp Met            
    1715                 1720                 1725                        

gat ttc  tca gcc ttg ctg ggc  tct ggc agc ggc agc  cgg gat tcc        5229
Asp Phe  Ser Ala Leu Leu Gly  Ser Gly Ser Gly Ser  Arg Asp Ser            
    1730                 1735                 1740                        

agg gaa  ggg atg ttt ttg ccg  aag cct gag gcc ggc  tcc gct att        5274
Arg Glu  Gly Met Phe Leu Pro  Lys Pro Glu Ala Gly  Ser Ala Ile            
    1745                 1750                 1755                        

agt gac  gtg ttt gag ggc cgc  gag gtg tgc cag cca  aaa cga atc        5319
Ser Asp  Val Phe Glu Gly Arg  Glu Val Cys Gln Pro  Lys Arg Ile            
    1760                 1765                 1770                        

cgg cca  ttt cat cct cca gga  agt cca tgg gcc aac  cgc cca ctc        5364
Arg Pro  Phe His Pro Pro Gly  Ser Pro Trp Ala Asn  Arg Pro Leu            
    1775                 1780                 1785                        

ccc gcc  agc ctc gca cca aca  cca acc ggt cca gta  cat gag cca        5409
Pro Ala  Ser Leu Ala Pro Thr  Pro Thr Gly Pro Val  His Glu Pro            
    1790                 1795                 1800                        

gtc ggg  tca ctg acc ccg gca  cca gtc cct cag cca  ctg gat cca        5454
Val Gly  Ser Leu Thr Pro Ala  Pro Val Pro Gln Pro  Leu Asp Pro            
    1805                 1810                 1815                        

gcg ccc  gca gtg act ccc gag  gcc agt cac ctg ttg  gag gat ccc        5499
Ala Pro  Ala Val Thr Pro Glu  Ala Ser His Leu Leu  Glu Asp Pro            
    1820                 1825                 1830                        

gat gaa  gag acg agc cag gct  gtc aaa gcc ctt cgg  gag atg gcc        5544
Asp Glu  Glu Thr Ser Gln Ala  Val Lys Ala Leu Arg  Glu Met Ala            
    1835                 1840                 1845                        

gat act  gtg att ccc cag aag  gaa gag gct gca atc  tgt ggc caa        5589
Asp Thr  Val Ile Pro Gln Lys  Glu Glu Ala Ala Ile  Cys Gly Gln            
    1850                 1855                 1860                        

atg gac  ctt tcc cat ccg ccc  cca agg ggc cat ctg  gat gag ctg        5634
Met Asp  Leu Ser His Pro Pro  Pro Arg Gly His Leu  Asp Glu Leu            
    1865                 1870                 1875                        

aca acc  aca ctt gag tcc atg  acc gag gat ctg aac  ctg gac tca        5679
Thr Thr  Thr Leu Glu Ser Met  Thr Glu Asp Leu Asn  Leu Asp Ser            
    1880                 1885                 1890                        

ccc ctg  acc ccg gaa ttg aac  gag att ctg gat acc  ttc ctg aac        5724
Pro Leu  Thr Pro Glu Leu Asn  Glu Ile Leu Asp Thr  Phe Leu Asn            
    1895                 1900                 1905                        

gac gag  tgc ctc ttg cat gcc  atg cat atc agc aca  gga ctg tcc        5769
Asp Glu  Cys Leu Leu His Ala  Met His Ile Ser Thr  Gly Leu Ser            
    1910                 1915                 1920                        

atc ttc  gac aca tct ctg ttt  tga                                     5793
Ile Phe  Asp Thr Ser Leu Phe                                              
    1925                 1930                                             


<210>  8
<211>  1930
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg 
1               5                   10                  15      


Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly 
            20                  25                  30          


Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro 
        35                  40                  45              


Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys 
    50                  55                  60                  


Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu 
65                  70                  75                  80  


Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys 
                85                  90                  95      


Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys 
            100                 105                 110         


Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu 
        115                 120                 125             


Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp 
    130                 135                 140                 


Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys 
145                 150                 155                 160 


Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu 
                165                 170                 175     


Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly 
            180                 185                 190         


Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu 
        195                 200                 205             


Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser 
    210                 215                 220                 


Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg 
225                 230                 235                 240 


Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly 
                245                 250                 255     


Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe 
            260                 265                 270         


Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys 
        275                 280                 285             


Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp 
    290                 295                 300                 


Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile 
305                 310                 315                 320 


Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro 
                325                 330                 335     


Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu 
            340                 345                 350         


Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys 
        355                 360                 365             


Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp 
    370                 375                 380                 


Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu 
385                 390                 395                 400 


Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu 
                405                 410                 415     


Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His 
            420                 425                 430         


Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp 
        435                 440                 445             


Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu 
    450                 455                 460                 


Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser 
465                 470                 475                 480 


Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp 
                485                 490                 495     


Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile 
            500                 505                 510         


Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu 
        515                 520                 525             


Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu 
    530                 535                 540                 


Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu 
545                 550                 555                 560 


Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn 
                565                 570                 575     


Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile 
            580                 585                 590         


Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn 
        595                 600                 605             


Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys 
    610                 615                 620                 


Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val 
625                 630                 635                 640 


Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu 
                645                 650                 655     


Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys 
            660                 665                 670         


Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn 
        675                 680                 685             


Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys 
    690                 695                 700                 


Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
705                 710                 715                 720 


Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln 
                725                 730                 735     


Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala 
            740                 745                 750         


Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val 
        755                 760                 765             


Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala 
    770                 775                 780                 


Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg 
785                 790                 795                 800 


Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu 
                805                 810                 815     


Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr 
            820                 825                 830         


Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu 
        835                 840                 845             


Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln 
    850                 855                 860                 


Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser 
865                 870                 875                 880 


Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val 
                885                 890                 895     


Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile 
            900                 905                 910         


Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu 
        915                 920                 925             


Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn 
945                 950                 955                 960 


Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile 
                965                 970                 975     


Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe 
            980                 985                 990         


Tyr Lys Val Arg Glu Ile Asn Asn  Tyr His His Ala His  Asp Ala Tyr 
        995                 1000                 1005             


Leu Asn  Ala Val Val Gly Thr  Ala Leu Ile Lys Lys  Tyr Pro Lys 
    1010                 1015                 1020             


Leu Glu  Ser Glu Phe Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val 
    1025                 1030                 1035             


Arg Lys  Met Ile Ala Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr 
    1040                 1045                 1050             


Ala Lys  Tyr Phe Phe Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr 
    1055                 1060                 1065             


Glu Ile  Thr Leu Ala Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile 
    1070                 1075                 1080             


Glu Thr  Asn Gly Glu Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg 
    1085                 1090                 1095             


Asp Phe  Ala Thr Val Arg Lys  Val Leu Ser Met Pro  Gln Val Asn 
    1100                 1105                 1110             


Ile Val  Lys Lys Thr Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu 
    1115                 1120                 1125             


Ser Ile  Leu Pro Lys Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys 
    1130                 1135                 1140             


Lys Asp  Trp Asp Pro Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr 
    1145                 1150                 1155             


Val Ala  Tyr Ser Val Leu Val  Val Ala Lys Val Glu  Lys Gly Lys 
    1160                 1165                 1170             


Ser Lys  Lys Leu Lys Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile 
    1175                 1180                 1185             


Met Glu  Arg Ser Ser Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu 
    1190                 1195                 1200             


Ala Lys  Gly Tyr Lys Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu 
    1205                 1210                 1215             


Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met 
    1220                 1225                 1230             


Leu Ala  Ser Ala Gly Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu 
    1235                 1240                 1245             


Pro Ser  Lys Tyr Val Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu 
    1250                 1255                 1260             


Lys Leu  Lys Gly Ser Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe 
    1265                 1270                 1275             


Val Glu  Gln His Lys His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile 
    1280                 1285                 1290             


Ser Glu  Phe Ser Lys Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp 
    1295                 1300                 1305             


Lys Val  Leu Ser Ala Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg 
    1310                 1315                 1320             


Glu Gln  Ala Glu Asn Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu 
    1325                 1330                 1335             


Gly Ala  Pro Ala Ala Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg 
    1340                 1345                 1350             


Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser 
    1370                 1375                 1380             


Gln Leu  Gly Gly Asp Ser Pro  Lys Lys Lys Arg Lys  Val Glu Ala 
    1385                 1390                 1395             


Ser Gly  Pro Ala Gly Ser Gly  Arg Ala Asp Ala Leu  Asp Asp Phe 
    1400                 1405                 1410             


Asp Leu  Asp Met Leu Gly Ser  Asp Ala Leu Asp Asp  Phe Asp Leu 
    1415                 1420                 1425             


Asp Met  Leu Gly Ser Asp Ala  Leu Asp Asp Phe Asp  Leu Asp Met 
    1430                 1435                 1440             


Leu Gly  Ser Asp Ala Leu Asp  Asp Phe Asp Leu Asp  Met Leu Ile 
    1445                 1450                 1455             


Asn Ser  Arg Ser Ser Gly Ser  Pro Lys Lys Lys Arg  Lys Val Gly 
    1460                 1465                 1470             


Ser Gln  Tyr Leu Pro Asp Thr  Asp Asp Arg His Arg  Ile Glu Glu 
    1475                 1480                 1485             


Lys Arg  Lys Arg Thr Tyr Glu  Thr Phe Lys Ser Ile  Met Lys Lys 
    1490                 1495                 1500             


Ser Pro  Phe Ser Gly Pro Thr  Asp Pro Arg Pro Pro  Pro Arg Arg 
    1505                 1510                 1515             


Ile Ala  Val Pro Ser Arg Ser  Ser Ala Ser Val Pro  Lys Pro Ala 
    1520                 1525                 1530             


Pro Gln  Pro Tyr Pro Phe Thr  Ser Ser Leu Ser Thr  Ile Asn Tyr 
    1535                 1540                 1545             


Asp Glu  Phe Pro Thr Met Val  Phe Pro Ser Gly Gln  Ile Ser Gln 
    1550                 1555                 1560             


Ala Ser  Ala Leu Ala Pro Ala  Pro Pro Gln Val Leu  Pro Gln Ala 
    1565                 1570                 1575             


Pro Ala  Pro Ala Pro Ala Pro  Ala Met Val Ser Ala  Leu Ala Gln 
    1580                 1585                 1590             


Ala Pro  Ala Pro Val Pro Val  Leu Ala Pro Gly Pro  Pro Gln Ala 
    1595                 1600                 1605             


Val Ala  Pro Pro Ala Pro Lys  Pro Thr Gln Ala Gly  Glu Gly Thr 
    1610                 1615                 1620             


Leu Ser  Glu Ala Leu Leu Gln  Leu Gln Phe Asp Asp  Glu Asp Leu 
    1625                 1630                 1635             


Gly Ala  Leu Leu Gly Asn Ser  Thr Asp Pro Ala Val  Phe Thr Asp 
    1640                 1645                 1650             


Leu Ala  Ser Val Asp Asn Ser  Glu Phe Gln Gln Leu  Leu Asn Gln 
    1655                 1660                 1665             


Gly Ile  Pro Val Ala Pro His  Thr Thr Glu Pro Met  Leu Met Glu 
    1670                 1675                 1680             


Tyr Pro  Glu Ala Ile Thr Arg  Leu Val Thr Gly Ala  Gln Arg Pro 
    1685                 1690                 1695             


Pro Asp  Pro Ala Pro Ala Pro  Leu Gly Ala Pro Gly  Leu Pro Asn 
    1700                 1705                 1710             


Gly Leu  Leu Ser Gly Asp Glu  Asp Phe Ser Ser Ile  Ala Asp Met 
    1715                 1720                 1725             


Asp Phe  Ser Ala Leu Leu Gly  Ser Gly Ser Gly Ser  Arg Asp Ser 
    1730                 1735                 1740             


Arg Glu  Gly Met Phe Leu Pro  Lys Pro Glu Ala Gly  Ser Ala Ile 
    1745                 1750                 1755             


Ser Asp  Val Phe Glu Gly Arg  Glu Val Cys Gln Pro  Lys Arg Ile 
    1760                 1765                 1770             


Arg Pro  Phe His Pro Pro Gly  Ser Pro Trp Ala Asn  Arg Pro Leu 
    1775                 1780                 1785             


Pro Ala  Ser Leu Ala Pro Thr  Pro Thr Gly Pro Val  His Glu Pro 
    1790                 1795                 1800             


Val Gly  Ser Leu Thr Pro Ala  Pro Val Pro Gln Pro  Leu Asp Pro 
    1805                 1810                 1815             


Ala Pro  Ala Val Thr Pro Glu  Ala Ser His Leu Leu  Glu Asp Pro 
    1820                 1825                 1830             


Asp Glu  Glu Thr Ser Gln Ala  Val Lys Ala Leu Arg  Glu Met Ala 
    1835                 1840                 1845             


Asp Thr  Val Ile Pro Gln Lys  Glu Glu Ala Ala Ile  Cys Gly Gln 
    1850                 1855                 1860             


Met Asp  Leu Ser His Pro Pro  Pro Arg Gly His Leu  Asp Glu Leu 
    1865                 1870                 1875             


Thr Thr  Thr Leu Glu Ser Met  Thr Glu Asp Leu Asn  Leu Asp Ser 
    1880                 1885                 1890             


Pro Leu  Thr Pro Glu Leu Asn  Glu Ile Leu Asp Thr  Phe Leu Asn 
    1895                 1900                 1905             


Asp Glu  Cys Leu Leu His Ala  Met His Ile Ser Thr  Gly Leu Ser 
    1910                 1915                 1920             


Ile Phe  Asp Thr Ser Leu Phe  
    1925                 1930 


<210>  9
<211>  306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct N-Split-Intein


<220>
<221>  CDS
<222>  (1)..(306)

<400>  9
tgc ctg tcc tac gag acc gaa atc ctg acc gtg gag tat ggg ctg ctg         48
Cys Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu           
1               5                   10                  15                

ccc atc ggc aag att gtg gag aag cgg att gaa tgc acc gtg tat agc         96
Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Cys Thr Val Tyr Ser           
            20                  25                  30                    

gtg gac aac aac ggc aac atc tac acc cag ccc gtg gct cag tgg cac        144
Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His           
        35                  40                  45                        

gac agg ggc gag cag gag gtg ttt gag tat tgt ctg gag gac ggc agc        192
Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser           
    50                  55                  60                            

ctg att aga gcc acc aaa gac cac aag ttc atg acc gtg gac ggg cag        240
Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln           
65                  70                  75                  80            

atg ctg ccc att gac gag att ttt gag cgg gaa ctt gac ctg atg cgg        288
Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg           
                85                  90                  95                

gtg gac aac ctg ccc aac                                                306
Val Asp Asn Leu Pro Asn                                                   
            100                                                           


<210>  10
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Cys Leu Ser Tyr Glu Thr Glu Ile Leu Thr Val Glu Tyr Gly Leu Leu 
1               5                   10                  15      


Pro Ile Gly Lys Ile Val Glu Lys Arg Ile Glu Cys Thr Val Tyr Ser 
            20                  25                  30          


Val Asp Asn Asn Gly Asn Ile Tyr Thr Gln Pro Val Ala Gln Trp His 
        35                  40                  45              


Asp Arg Gly Glu Gln Glu Val Phe Glu Tyr Cys Leu Glu Asp Gly Ser 
    50                  55                  60                  


Leu Ile Arg Ala Thr Lys Asp His Lys Phe Met Thr Val Asp Gly Gln 
65                  70                  75                  80  


Met Leu Pro Ile Asp Glu Ile Phe Glu Arg Glu Leu Asp Leu Met Arg 
                85                  90                  95      


Val Asp Asn Leu Pro Asn 
            100         


<210>  11
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct C-Split-Intein


<220>
<221>  CDS
<222>  (1)..(57)

<400>  11
cat cgg cgt gga gag aga cca caa ctt cgc cct gaa gaa cgg ctt cat         48
His Arg Arg Gly Glu Arg Pro Gln Leu Arg Pro Glu Glu Arg Leu His           
1               5                   10                  15                

cgc cag caa c                                                           58
Arg Gln Gln                                                               
                                                                          


<210>  12
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

His Arg Arg Gly Glu Arg Pro Gln Leu Arg Pro Glu Glu Arg Leu His 
1               5                   10                  15      


Arg Gln Gln 
            


<210>  13
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct MS2


<220>
<221>  CDS
<222>  (1)..(390)

<400>  13
atg gct tca aac ttt act cag ttc gtg ctc gtg gac aat ggt ggg aca         48
Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr           
1               5                   10                  15                

ggg gat gtg aca gtg gct cct tct aat ttc gct aat ggg gtg gca gag         96
Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu           
            20                  25                  30                    

tgg atc agc tcc aac tca cgg agc cag gcc tac aag gtg aca tgc agc        144
Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser           
        35                  40                  45                        

gtc agg cag tct agt gcc cag aag aga aag tat acc atc aag gtg gag        192
Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu           
    50                  55                  60                            

gtc ccc aaa gtg gct acc cag aca gtg ggc gga gtc gaa ctg cct gtc        240
Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val           
65                  70                  75                  80            

gcc gct tgg agg tcc tac ctg aac atg gag ctc act atc cca att ttc        288
Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe           
                85                  90                  95                

gct acc aat tct gac tgt gaa ctc atc gtg aag gca atg cag ggg ctc        336
Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu           
            100                 105                 110                   

ctc aaa gac ggt aat cct atc cct tcc gcc atc gcc gct aac tca ggt        384
Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly           
        115                 120                 125                       

atc tac                                                                390
Ile Tyr                                                                   
    130                                                                   


<210>  14
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr 
    130 


<210>  15
<211>  1032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct MS2-p65


<220>
<221>  CDS
<222>  (1)..(1032)

<400>  15
atg gct tca aac ttt act cag ttc gtg ctc gtg gac aat ggt ggg aca         48
Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr           
1               5                   10                  15                

ggg gat gtg aca gtg gct cct tct aat ttc gct aat ggg gtg gca gag         96
Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu           
            20                  25                  30                    

tgg atc agc tcc aac tca cgg agc cag gcc tac aag gtg aca tgc agc        144
Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser           
        35                  40                  45                        

gtc agg cag tct agt gcc cag aag aga aag tat acc atc aag gtg gag        192
Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu           
    50                  55                  60                            

gtc ccc aaa gtg gct acc cag aca gtg ggc gga gtc gaa ctg cct gtc        240
Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val           
65                  70                  75                  80            

gcc gct tgg agg tcc tac ctg aac atg gag ctc act atc cca att ttc        288
Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe           
                85                  90                  95                

gct acc aat tct gac tgt gaa ctc atc gtg aag gca atg cag ggg ctc        336
Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu           
            100                 105                 110                   

ctc aaa gac ggt aat cct atc cct tcc gcc atc gcc gct aac tca ggt        384
Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly           
        115                 120                 125                       

atc tac agc gct gga gga ggt gga agc gga gga gga gga agc gga gga        432
Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly           
    130                 135                 140                           

gga ggt agc gga cct aag aaa aag agg aag gtg gcg acc gct gga tcc        480
Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Thr Ala Gly Ser           
145                 150                 155                 160           

cct tca ggg cag atc agc aac cag gcc ctg gct ctg gcc cct agc tcc        528
Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser           
                165                 170                 175               

gct cca gtg ctg gcc cag act atg gtg ccc tct agt gct atg gtg cct        576
Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro           
            180                 185                 190                   

ctg gcc cag cca cct gct cca gcc cct gtg ctg acc cca gga cca ccc        624
Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro           
        195                 200                 205                       

cag tca ctg agc gct cca gtg ccc aag tct aca cag gcc ggc gag ggg        672
Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly           
    210                 215                 220                           

act ctg agt gaa gct ctg ctg cac ctg cag ttc gac gct gat gag gac        720
Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp           
225                 230                 235                 240           

ctg gga gct ctg ctg ggg aac agc acc gat ccc gga gtg ttc aca gat        768
Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp           
                245                 250                 255               

ctg gcc tcc gtg gac aac tct gag ttt cag cag ctg ctg aat cag ggc        816
Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly           
            260                 265                 270                   

gtg tcc atg tct cat agt aca gcc gaa cca atg ctg atg gag tac ccc        864
Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro           
        275                 280                 285                       

gaa gcc att acc cgg ctg gtg acc ggc agc cag cgg ccc ccc gac ccc        912
Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro           
    290                 295                 300                           

gct cca act ccc ctg gga acc agc ggc ctg cct aat ggg ctg tcc gga        960
Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly           
305                 310                 315                 320           

gat gaa gac ttc tca agc atc gct gat atg gac ttt agt gcc ctg ctg       1008
Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu           
                325                 330                 335               

tca cag att tcc tct agt ggg cag                                       1032
Ser Gln Ile Ser Ser Ser Gly Gln                                           
            340                                                           


<210>  16
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Thr Ala Gly Ser 
145                 150                 155                 160 


Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro 
            180                 185                 190         


Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro 
        195                 200                 205             


Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly 
    210                 215                 220                 


Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp 
225                 230                 235                 240 


Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp 
                245                 250                 255     


Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly 
            260                 265                 270         


Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro 
        275                 280                 285             


Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro 
    290                 295                 300                 


Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly 
305                 310                 315                 320 


Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu 
                325                 330                 335     


Ser Gln Ile Ser Ser Ser Gly Gln 
            340                 


<210>  17
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct MS2-p65-HSF1


<220>
<221>  CDS
<222>  (1)..(1470)

<400>  17
atg gct tca aac ttt act cag ttc gtg ctc gtg gac aat ggt ggg aca         48
Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr           
1               5                   10                  15                

ggg gat gtg aca gtg gct cct tct aat ttc gct aat ggg gtg gca gag         96
Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu           
            20                  25                  30                    

tgg atc agc tcc aac tca cgg agc cag gcc tac aag gtg aca tgc agc        144
Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser           
        35                  40                  45                        

gtc agg cag tct agt gcc cag aag aga aag tat acc atc aag gtg gag        192
Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu           
    50                  55                  60                            

gtc ccc aaa gtg gct acc cag aca gtg ggc gga gtc gaa ctg cct gtc        240
Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val           
65                  70                  75                  80            

gcc gct tgg agg tcc tac ctg aac atg gag ctc act atc cca att ttc        288
Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe           
                85                  90                  95                

gct acc aat tct gac tgt gaa ctc atc gtg aag gca atg cag ggg ctc        336
Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu           
            100                 105                 110                   

ctc aaa gac ggt aat cct atc cct tcc gcc atc gcc gct aac tca ggt        384
Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly           
        115                 120                 125                       

atc tac agc gct gga gga ggt gga agc gga gga gga gga agc gga gga        432
Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly           
    130                 135                 140                           

gga ggt agc gga cct aag aaa aag agg aag gtg gcg acc gct gga tcc        480
Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Thr Ala Gly Ser           
145                 150                 155                 160           

cct tca ggg cag atc agc aac cag gcc ctg gct ctg gcc cct agc tcc        528
Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser           
                165                 170                 175               

gct cca gtg ctg gcc cag act atg gtg ccc tct agt gct atg gtg cct        576
Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro           
            180                 185                 190                   

ctg gcc cag cca cct gct cca gcc cct gtg ctg acc cca gga cca ccc        624
Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro           
        195                 200                 205                       

cag tca ctg agc gct cca gtg ccc aag tct aca cag gcc ggc gag ggg        672
Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly           
    210                 215                 220                           

act ctg agt gaa gct ctg ctg cac ctg cag ttc gac gct gat gag gac        720
Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp           
225                 230                 235                 240           

ctg gga gct ctg ctg ggg aac agc acc gat ccc gga gtg ttc aca gat        768
Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp           
                245                 250                 255               

ctg gcc tcc gtg gac aac tct gag ttt cag cag ctg ctg aat cag ggc        816
Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly           
            260                 265                 270                   

gtg tcc atg tct cat agt aca gcc gaa cca atg ctg atg gag tac ccc        864
Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro           
        275                 280                 285                       

gaa gcc att acc cgg ctg gtg acc ggc agc cag cgg ccc ccc gac ccc        912
Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro           
    290                 295                 300                           

gct cca act ccc ctg gga acc agc ggc ctg cct aat ggg ctg tcc gga        960
Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly           
305                 310                 315                 320           

gat gaa gac ttc tca agc atc gct gat atg gac ttt agt gcc ctg ctg       1008
Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu           
                325                 330                 335               

tca cag att tcc tct agt ggg cag gga gga ggt gga agc ggc ttc agc       1056
Ser Gln Ile Ser Ser Ser Gly Gln Gly Gly Gly Gly Ser Gly Phe Ser           
            340                 345                 350                   

gtg gac acc agt gcc ctg ctg gac ctg ttc agc ccc tcg gtg acc gtg       1104
Val Asp Thr Ser Ala Leu Leu Asp Leu Phe Ser Pro Ser Val Thr Val           
        355                 360                 365                       

ccc gac atg agc ctg cct gac ctt gac agc agc ctg gcc agt atc caa       1152
Pro Asp Met Ser Leu Pro Asp Leu Asp Ser Ser Leu Ala Ser Ile Gln           
    370                 375                 380                           

gag ctc ctg tct ccc cag gag ccc ccc agg cct ccc gag gca gag aac       1200
Glu Leu Leu Ser Pro Gln Glu Pro Pro Arg Pro Pro Glu Ala Glu Asn           
385                 390                 395                 400           

agc agc ccg gat tca ggg aag cag ctg gtg cac tac aca gcg cag ccg       1248
Ser Ser Pro Asp Ser Gly Lys Gln Leu Val His Tyr Thr Ala Gln Pro           
                405                 410                 415               

ctg ttc ctg ctg gac ccc ggc tcc gtg gac acc ggg agc aac gac ctg       1296
Leu Phe Leu Leu Asp Pro Gly Ser Val Asp Thr Gly Ser Asn Asp Leu           
            420                 425                 430                   

ccg gtg ctg ttt gag ctg gga gag ggc tcc tac ttc tcc gaa ggg gac       1344
Pro Val Leu Phe Glu Leu Gly Glu Gly Ser Tyr Phe Ser Glu Gly Asp           
        435                 440                 445                       

ggc ttc gcc gag gac ccc acc atc tcc ctg ctg aca ggc tcg gag cct       1392
Gly Phe Ala Glu Asp Pro Thr Ile Ser Leu Leu Thr Gly Ser Glu Pro           
    450                 455                 460                           

ccc aaa gcc aag gac ccc act gtc tcc tgt aca tcc ggg ttt gcc aac       1440
Pro Lys Ala Lys Asp Pro Thr Val Ser Cys Thr Ser Gly Phe Ala Asn           
465                 470                 475                 480           

gaa cta ggc ccg cga ctt atg ggt aaa taa                               1470
Glu Leu Gly Pro Arg Leu Met Gly Lys                                       
                485                                                       


<210>  18
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Thr Ala Gly Ser 
145                 150                 155                 160 


Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro 
            180                 185                 190         


Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro 
        195                 200                 205             


Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly 
    210                 215                 220                 


Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp 
225                 230                 235                 240 


Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp 
                245                 250                 255     


Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly 
            260                 265                 270         


Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro 
        275                 280                 285             


Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro 
    290                 295                 300                 


Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly 
305                 310                 315                 320 


Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu 
                325                 330                 335     


Ser Gln Ile Ser Ser Ser Gly Gln Gly Gly Gly Gly Ser Gly Phe Ser 
            340                 345                 350         


Val Asp Thr Ser Ala Leu Leu Asp Leu Phe Ser Pro Ser Val Thr Val 
        355                 360                 365             


Pro Asp Met Ser Leu Pro Asp Leu Asp Ser Ser Leu Ala Ser Ile Gln 
    370                 375                 380                 


Glu Leu Leu Ser Pro Gln Glu Pro Pro Arg Pro Pro Glu Ala Glu Asn 
385                 390                 395                 400 


Ser Ser Pro Asp Ser Gly Lys Gln Leu Val His Tyr Thr Ala Gln Pro 
                405                 410                 415     


Leu Phe Leu Leu Asp Pro Gly Ser Val Asp Thr Gly Ser Asn Asp Leu 
            420                 425                 430         


Pro Val Leu Phe Glu Leu Gly Glu Gly Ser Tyr Phe Ser Glu Gly Asp 
        435                 440                 445             


Gly Phe Ala Glu Asp Pro Thr Ile Ser Leu Leu Thr Gly Ser Glu Pro 
    450                 455                 460                 


Pro Lys Ala Lys Asp Pro Thr Val Ser Cys Thr Ser Gly Phe Ala Asn 
465                 470                 475                 480 


Glu Leu Gly Pro Arg Leu Met Gly Lys 
                485                 


<210>  19
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct VP16


<220>
<221>  CDS
<222>  (1)..(39)

<400>  19
gac gca ttg gac gat ttt gat ctg gat atg ctg gga agt                     39
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser                       
1               5                   10                                    


<210>  20
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20

Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser 
1               5                   10              


<210>  21
<211>  10639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct dCas9-SAM-P2A-VPR

<400>  21
ctagggcgcg ccggattcga cattgattat tgactagtta ttaatagtaa tcaattacgg       60

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc      120

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca      180

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg      240

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg      300

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt      360

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct      420

gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt      480

aattattttg tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg      540

cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc      600

gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga      660

agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc      720

tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac      780

ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg      840

tggctgcgtg aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc      900

ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc      960

ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga     1020

gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt     1080

gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc     1140

cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc     1200

gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc     1260

cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg     1320

gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg     1380

cgagagggcg cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc     1440

cgccgcaccc cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg     1500

gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg     1560

gctgccgcag ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg     1620

gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct     1680

acagatcctt aattaaccgt cttaagcctg taacaaccgg tacagttcga ataacttcgt     1740

atagcataca ttatacgaag ttataagctt gcatgcctgc aggtcggccg ccacgaccgg     1800

ccggtgccgc caccatcccc tgacccacgc ccctgacccc tcacaaggag acgaccttcc     1860

atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta     1920

cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac     1980

cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac     2040

atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag     2100

agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt     2160

tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag     2220

cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc     2280

agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg     2340

gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc     2400

gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga     2460

cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tggctccgac     2520

cgaagccacc cggggcggcc ccgccgaccc cgcacccgcc cccgaggccc accgactcta     2580

gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc     2640

acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat     2700

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     2760

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg     2820

gatccataac ttcgtatagc atacattata cgaagttata ccgggccacc atagtcgcga     2880

gtagcttggg ccagctaggc cttgaccaaa gttcctctgg aattgcgatc gcgaagttcc     2940

tattctctag aaagtatagg aacttcgtgc cgccaccatg gactacaagg acgacgacga     3000

taaggcttca aactttactc agttcgtgct cgtggacaat ggtgggacag gggatgtgac     3060

agtggctcct tctaatttcg ctaatggggt ggcagagtgg atcagctcca actcacggag     3120

ccaggcctac aaggtgacat gcagcgtcag gcagtctagt gcccagaaga gaaagtatac     3180

catcaaggtg gaggtcccca aagtggctac ccagacagtg ggcggagtcg aactgcctgt     3240

cgccgcttgg aggtcctacc tgaacatgga gctcactatc ccaattttcg ctaccaattc     3300

tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc aaagacggta atcctatccc     3360

ttccgccatc gccgctaact caggtatcta cagcgctgga ggaggtggaa gcggaggagg     3420

aggaagcgga ggaggaggta gcggacctaa gaaaaagagg aaggtggcgg ccgctggatc     3480

cccttcaggg cagatcagca accaggccct ggctctggcc cctagctccg ctccagtgct     3540

ggcccagact atggtgccct ctagtgctat ggtgcctctg gcccagccac ctgctccagc     3600

ccctgtgctg accccaggac caccccagtc actgagcgct ccagtgccca agtctacaca     3660

ggccggcgag gggactctga gtgaagctct gctgcacctg cagttcgacg ctgatgagga     3720

cctgggagct ctgctgggga acagcaccga tcccggagtg ttcacagatc tggcctccgt     3780

ggacaactct gagtttcagc agctgctgaa tcagggcgtg tccatgtctc atagtacagc     3840

cgaaccaatg ctgatggagt accccgaagc cattacccgg ctggtgaccg gcagccagcg     3900

gccccccgac cccgctccaa ctcccctggg aaccagcggc ctgcctaatg ggctgtccgg     3960

agatgaagac ttctcaagca tcgctgatat ggactttagt gccctgctgt cacagatttc     4020

ctctagtggg cagggaggag gtggaagcgg cttcagcgtg gacaccagtg ccctgctgga     4080

cctgttcagc ccctcggtga ccgtgcccga catgagcctg cctgaccttg acagcagcct     4140

ggccagtatc caagagctcc tgtctcccca ggagcccccc aggcctcccg aggcagagaa     4200

cagcagcccg gattcaggga agcagctggt gcactacaca gcgcagccgc tgttcctgct     4260

ggaccccggc tccgtggaca ccgggagcaa cgacctgccg gtgctgtttg agctgggaga     4320

gggctcctac ttctccgaag gggacggctt cgccgaggac cccaccatct ccctgctgac     4380

aggctcggag cctcccaaag ccaaggaccc cactgtctcc cgaagttcct attctctaga     4440

aagtatagga acttcgggca gcggcgccac caacttcagc ctgctgaaac aggctggcga     4500

cgtcgaggaa aatcccgggc catgtacaat gtacccatac gatgttccag attacgcttc     4560

gccgaagaaa aagcgcaagg tcgaagcgtc cgacaagaag tacagcatcg gcctggccat     4620

cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa     4680

attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct     4740

gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag     4800

atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc     4860

caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa     4920

gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa     4980

gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct     5040

gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga     5100

gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac     5160

ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat     5220

cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg     5280

cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc tgacccccaa     5340

cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta     5400

cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct     5460

ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga     5520

gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga     5580

cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt     5640

cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga     5700

gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt     5760

gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc     5820

ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc     5880

attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta     5940

cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga     6000

aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt     6060

catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca     6120

cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac     6180

cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct     6240

gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa     6300

aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct     6360

gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga     6420

aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat     6480

gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct     6540

gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg     6600

ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag     6660

aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc     6720

ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc     6780

cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat     6840

gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca     6900

gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct     6960

gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct     7020

gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa     7080

ccggctgtcc gactacgatg tggacgccat cgtgcctcag agctttctga aggacgactc     7140

catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc     7200

ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct     7260

gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact     7320

ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt     7380

ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg     7440

ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca     7500

gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc     7560

cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg     7620

cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa     7680

ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac     7740

cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga     7800

gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca     7860

agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct     7920

gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta     7980

cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa     8040

gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag     8100

aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa     8160

aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa     8220

gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa     8280

atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga     8340

taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga     8400

gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct     8460

gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca     8520

cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat     8580

cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag     8640

catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acagccccaa     8700

gaagaagaga aaggtggagg ccagcgggcc ggccggatcc ggacgggctg acgcattgga     8760

cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct     8820

tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga     8880

tttcgacctg gacatgctga ttaactctag aagttccgga tctccgaaaa agaaacgcaa     8940

agttggtagc cagtacctgc ccgacaccga cgaccggcac cggatcgagg aaaagcggaa     9000

gcggacctac gagacattca agagcatcat gaagaagtcc cccttcagcg gccccaccga     9060

ccctagacct ccacctagaa gaatcgccgt gcccagcaga tccagcgcca gcgtgccaaa     9120

acctgccccc cagccttacc ccttcaccag cagcctgagc accatcaact acgacgagtt     9180

ccctaccatg gtgttcccca gcggccagat ctctcaggcc tctgctctgg ctccagcccc     9240

tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct ccagccatgg tgtctgcact     9300

ggctcaggca ccagcacccg tgcctgtgct ggctcctgga cctccacagg ctgtggctcc     9360

accagcccct aaacctacac aggccggcga gggcacactg tctgaagctc tgctgcagct     9420

gcagttcgac gacgaggatc tgggagccct gctgggaaac agcaccgatc ctgccgtgtt     9480

caccgacctg gccagcgtgg acaacagcga gttccagcag ctgctgaacc agggcatccc     9540

tgtggcccct cacaccaccg agcccatgct gatggaatac cccgaggcca tcacccggct     9600

cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc cctctgggag caccaggcct     9660

gcctaatgga ctgctgtctg gcgacgagga cttcagctct atcgccgata tggatttctc     9720

agccttgctg ggctctggca gcggcagccg ggattccagg gaagggatgt ttttgccgaa     9780

gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc cgcgaggtgt gccagccaaa     9840

acgaatccgg ccatttcatc ctccaggaag tccatgggcc aaccgcccac tccccgccag     9900

cctcgcacca acaccaaccg gtccagtaca tgagccagtc gggtcactga ccccggcacc     9960

agtccctcag ccactggatc cagcgcccgc agtgactccc gaggccagtc acctgttgga    10020

ggatcccgat gaagagacga gccaggctgt caaagccctt cgggagatgg ccgatactgt    10080

gattccccag aaggaagagg ctgcaatctg tggccaaatg gacctttccc atccgccccc    10140

aaggggccat ctggatgagc tgacaaccac acttgagtcc atgaccgagg atctgaacct    10200

ggactcaccc ctgaccccgg aattgaacga gattctggat accttcctga acgacgagtg    10260

cctcttgcat gccatgcata tcagcacagg actgtccatc ttcgacacat ctctgttttg    10320

aacgcgtaaa tgattgcaga tccactagtt ctagagctcg ctgatcagcc tcgactgtgc    10380

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag    10440

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta    10500

ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag    10560

acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca    10620

gctggggctc gaggctagc                                                 10639


<210>  22
<211>  4933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct AAV-ALN-flexGFP

<400>  22
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccgca cgcgtagagg gcctatttcc catgattcct      180

tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta      240

aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt      300

gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat      360

ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggga gccgctcgct      420

gcagcagcgg ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca      480

agttaaaata aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca      540

gggccaagtg gcaccgagtc ggtgcttttt ttcggtgctt ttttttgaac gctgacgtca      600

tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt      660

gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat      720

gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt      780

ataagttctg tatgagacca ctctttccca gggggctgaa tggagagttt gcagttttag      840

agctaggcca acatgaggat cacccatgtc tgcagggcct agcaagttaa aataaggcta      900

gtccgttatc aacttggcca acatgaggat cacccatgtc tgcagggcca agtggcaccg      960

agtcggtgct ttttttcggt gctttttttt gatccgacgc cgccatctct aggcccgcgc     1020

cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg     1080

catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt     1140

tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat     1200

actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt     1260

gtgtgttttg agactataaa tatcccttgg agaaaagcct tgtttggggg agcaaaggag     1320

tcgccttggt tttagagcta ggccaacatg aggatcaccc atgtctgcag ggcctagcaa     1380

gttaaaataa ggctagtccg ttatcaactt ggccaacatg aggatcaccc atgtctgcag     1440

ggccaagtgg caccgagtcg gtgctttttt tcggtgcttt tttttctgca gtatttagca     1500

tgccccaccc atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt     1560

ttatctcaaa ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat     1620

tttagttaaa tttcctgctg aagctctagt acgataagca acttgaccta agtgtaaagt     1680

tgagacttcc ttcaggttta tatagcttgt gcgccgcttg ggtacctcgg aatgcatcca     1740

agagtgaacc gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc     1800

aagttaaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc     1860

agggccaagt ggcaccgagt cggtgctttt tttgaacgct gacgtcatca acccgctcca     1920

aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg     1980

aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt     2040

ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agttctgtat     2100

gagaccactc tttcccaggg gcggtgggtc attgtttccg gttttagagc taggccaaca     2160

tgaggatcac ccatgtctgc agggcctagc aagttaaaat aaggctagtc cgttatcaac     2220

ttggccaaca tgaggatcac ccatgtctgc agggccaagt ggcaccgagt cggtgctttt     2280

tttcggtgct tttttttctg cagtatttag catgccccac ccatctgcaa ggcattctgg     2340

atagtgtcaa aacagccgga aatcaagtcc gtttatctca aactttagca ttttgggaat     2400

aaatgatatt tgctatgctg gttaaattag attttagtta aatttcctgc tgaagctcta     2460

gtacgataag caacttgacc taagtgtaaa gttgagactt ccttcaggtt tatatagctt     2520

gtgcgccgct tgggtacctc ggtgccagtg acgccggcct gggttttaga gctaggccaa     2580

catgaggatc acccatgtct gcagggccta gcaagttaaa ataaggctag tccgttatca     2640

acttggccaa catgaggatc acccatgtct gcagggccaa gtggcaccga gtcggtgctt     2700

tttttgagct gtactgagac gcacgtgcgc cattgacgtc aataatgacg tatgttccca     2760

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg     2820

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg     2880

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt     2940

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct     3000

gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt     3060

aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg     3120

ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg     3180

gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc     3240

gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc     3300

gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg     3360

cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct     3420

tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc     3480

ggctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt     3540

cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt     3600

ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa     3660

agaattggat ccccataact tcgtataaag tatcctatac gaagttatat caaaatagga     3720

agaccaatgc ttcaccatcg acccgaattg ccaagcatca ccatcgaccc ataacttcgt     3780

ataatgtatg ctatacgaag ttatactagg gatcccccct cgagttttac ttgtacagct     3840

cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc     3900

gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca     3960

gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc     4020

tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt     4080

cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt     4140

tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct     4200

cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct     4260

cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg     4320

ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca     4380

cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc     4440

cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca     4500

ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg gcggtaccga     4560

attctagtat aacttcgtat aggatacttt atacgaagtt atcattggga ttcttcctat     4620

tttgatccaa gcatcaccat cgaccctcta gtccagatct caccatcgac ccataacttc     4680

gtatagcata cattatacga agttatgtcc ctcgaagagg ttcgaattcg ataataaagg     4740

aaatttattt tcattgcaat agtgtgttgg ttttttgtgt gcgagcggcc gcaggaaccc     4800

ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga     4860

ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc     4920

agctgcctgc agg                                                        4933


<210>  23
<211>  3850
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct AAV-N-flex-dCas9aa1-573-N-intein

<400>  23
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct gcggccgcaa ggtcgggcag gaagagggcc tatttcccat gattccttca      180

tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac      240

acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca      300

gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc      360

gatttcttgg ctttatatat cttgtggaaa ggacgaaata cctcgggagc cgctcgctgc      420

agcagcggtt ttagagctag gccaacatga ggatcaccca tgtctgcagg gcctagcaag      480

ttaaaataag gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg      540

gccaagtggc accgagtcgg tgcttttttt tgagcgacct gtacccgtta cataacttac      600

ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac      660

gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt      720

ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa      780

atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta      840

catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac      900

tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt      960

ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg     1020

cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct     1080

ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc     1140

gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct     1200

cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg     1260

gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg     1320

ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg     1380

ttggatcctt aattaaataa cttcgtatag catacattat acgaagttat agtcataact     1440

tcgtatagta tacattatac gaagttatgt tgggcaggtt gtccacccgc atcaggtcaa     1500

gttcccgctc aaaaatctcg tcaatgggca gcatctgccc gtccacggtc atgaacttgt     1560

ggtctttggt ggctctaatc aggctgccgt cctccagaca atactcaaac acctcctgct     1620

cgcccctgtc gtgccactga gccacgggct gggtgtagat gttgccgttg ttgtccacgc     1680

tatacacggt gcattcaatc cgcttctcca caatcttgcc gatgggcagc agcccatact     1740

ccacggtcag gatttcggtc tcgtaggaca ggcactcgat tttcttgaag tagtcctctt     1800

tcagctgctt cacggtcact ttccggttgg tcttgaacag caggtccacg atggcctttt     1860

tctgctcgcc gctcaggaag gcgggctttc tcattccctc ggtcacgtat ttcactttgg     1920

tcagctcgtt atacacggtg aagtactcgt acagcaggct gtgcttgggc agcaccttct     1980

cgttgggcag gttcttatcg aagttggtca tccgctcgat gaagctctgg gcggaagcgc     2040

ccttgtccac cacttcctcg aagttccagg gggtgatggt ttcctcgctc tttctggtca     2100

tccaggcgaa tctgctgttt cccctggcca gagggcccac gtagtagggg atgcggaagg     2160

tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa tgggtaaaaa tcttcctgcc     2220

gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg ggggatgctg ccgttgtcga     2280

aggtccgctg cttccgcagc aggtcctctc tgttcagctt cacgagcagt tcctcggtgc     2340

cgtccatctt ttccaggatg ggcttgatga acttgtagaa ctcttcctgg ctggctccgc     2400

cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa gaaaatctct ttgtacttct     2460

caggcagctg ctgccgcacg agagctttca gcagggtcag gtcctggtgg tgctcgtcgt     2520

atctcttgat catagaggcg ctcagggggg ccttggtgat ctcggtgttc actctcagga     2580

tgtcgctcag caggatggcg tcggacaggt tcttggcggc cagaaacagg tcggcgtact     2640

ggtcgccgat ctgggccagc aggttgtcca ggtcgtcgtc gtaggtgtcc ttgctcagct     2700

gcagtttggc atcctcggcc aggtcgaagt tgctcttgaa gttgggggtc aggcccaggc     2760

tcagggcaat caggttgccg aacaggccat tcttcttctc gccgggcagc tgggcgatca     2820

gattttccag ccgtctgctc ttgctcagtc tggcagacag gatggccttg gcgtccacgc     2880

cgctggcgtt gatggggttt tcctcgaaca gctggttgta ggtctgcacc agctggatga     2940

acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc ctcgatcagg aagtggcccc     3000

ggaacttgat catgtgggcc agggccagat agatcagccg caggtcggcc ttgtcggtgc     3060

tgtccaccag tttctttctc aggtggtaga tggtggggta cttctcgtgg taggccacct     3120

cgtccacgat gttgccgaag atggggtgcc gctcgtgctt cttatcctct tccaccagga     3180

aggactcttc cagtctgtgg aagaagctgt cgtccacctt ggccatctcg ttgctgaaga     3240

tctcttgcag atagcagatc cggttcttcc gtctggtgta tcttcttctg gcggttctct     3300

tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag cagggctccg atcaggttct     3360

tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg ggcaccttgt     3420

actcgtcggt gatcacggcc cagcccacag agttggtgcc gatggccagg ccgatgctgt     3480

acttcttgtc ggctgctggg actccgtgga tacctttatc gtcgtcatcc ttgtagtcag     3540

cggccgccac cttcctcttt ttcttaggtc ccatggtggc ggcataactt cgtataatgt     3600

atgctatacg aagttatagt cataacttcg tataatgtat actatacgaa gttattgaac     3660

gcgtaataaa ggaaatttat tttcattgca atagtgtgtt ggttttttgt gtgcggccgc     3720

aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg     3780

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc     3840

gagcgcgcag                                                            3850


<210>  24
<211>  4406
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct AAV-C-dCas9aa574-1368-VP64-C-intein

<400>  24
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct gcggccgcaa ggtcgggcag gaagagggcc tatttcccat gattccttca      180

tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac      240

acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca      300

gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc      360

gatttcttgg ctttatatat cttgtggaaa ggacgaaata cctcggggct gaatggagag      420

tttgcagttt tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt      480

taaaataagg ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg      540

ccaagtggca ccgagtcggt gctttttttt gagcgacctg tacccgttac ataacttacg      600

gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg      660

ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg      720

gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa      780

tggcccgcct ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac      840

atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact      900

ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt      960

tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc     1020

gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc     1080

cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg     1140

cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg ccgccgcctc     1200

gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg     1260

cccttctcct ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt     1320

tggtggggta ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt     1380

tggatcctta attaataata cgactcacta taggggccgc caccatgata aagattgcca     1440

ccagaaagta tctgggcaag cagaacgtgt atgacatcgg cgtggagaga gaccacaact     1500

tcgccctgaa gaacggcttc atcgccagca actgcttcga ctccgtggaa atctccggcg     1560

tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg     1620

acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc     1680

tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt     1740

tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga     1800

gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc     1860

tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga     1920

cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc     1980

acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg     2040

tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa     2100

tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc     2160

ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa     2220

acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt     2280

acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc     2340

ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaagg     2400

cccggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact     2460

ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg     2520

ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg     2580

aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt     2640

acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg     2700

tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc     2760

acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta     2820

agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg     2880

ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca     2940

tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga     3000

tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg     3060

tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag     3120

gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa     3180

agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg     3240

tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc     3300

tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg     3360

aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc     3420

tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg     3480

gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg     3540

agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca     3600

agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg     3660

ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca     3720

gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg     3780

ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc     3840

tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt     3900

ctcagctggg aggcgacgcc tatccctatg acgtgcccga ttatgccagc ctgggcagcg     3960

gctcacccaa gaagaagagg aaggtggctg caggaggcgg tggaagcggg cgcgccgacg     4020

cattggacga ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg     4080

acatgcttgg ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc     4140

ttgatgattt cgacctggac atgctgatta actctagaag ttccggatct ccgaaaaaga     4200

aacgcaaagt ttgaacgcgt aataaaggaa atttattttc attgcaatag tgtgttggtt     4260

ttttgtgtgc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct     4320

cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg     4380

gcctcagtga gcgagcgagc gcgcag                                          4406


<210>  25
<211>  4377
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct AAV-Lmx1a-Nr4a2-SAM

<400>  25
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct gcggccgccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc      180

caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca      240

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      300

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta      360

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      420

catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc      480

cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg      540

gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag      600

aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg      660

gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct      720

gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga      780

ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc      840

tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc      900

tggagcacct gcctgaaatc actttttttc aggttggagc gatcgcgccg ccaccatggc      960

ttcaaacttt actcagttcg tgctcgtgga caatggtggg acaggggatg tgacagtggc     1020

tccttctaat ttcgctaatg gggtggcaga gtggatcagc tccaactcac ggagccaggc     1080

ctacaaggtg acatgcagcg tcaggcagtc tagtgcccag aagagaaagt ataccatcaa     1140

ggtggaggtc cccaaagtgg ctacccagac agtgggcgga gtcgaactgc ctgtcgccgc     1200

ttggaggtcc tacctgaaca tggagctcac tatcccaatt ttcgctacca attctgactg     1260

tgaactcatc gtgaaggcaa tgcaggggct cctcaaagac ggtaatccta tcccttccgc     1320

catcgccgct aactcaggta tctacagcgc tggaggaggt ggaagcggag gaggaggaag     1380

cggaggagga ggtagcggac ctaagaaaaa gaggaaggtg gcgaccgctg gatccccttc     1440

agggcagatc agcaaccagg ccctggctct ggcccctagc tccgctccag tgctggccca     1500

gactatggtg ccctctagtg ctatggtgcc tctggcccag ccacctgctc cagcccctgt     1560

gctgacccca ggaccacccc agtcactgag cgctccagtg cccaagtcta cacaggccgg     1620

cgaggggact ctgagtgaag ctctgctgca cctgcagttc gacgctgatg aggacctggg     1680

agctctgctg gggaacagca ccgatcccgg agtgttcaca gatctggcct ccgtggacaa     1740

ctctgagttt cagcagctgc tgaatcaggg cgtgtccatg tctcatagta cagccgaacc     1800

aatgctgatg gagtaccccg aagccattac ccggctggtg accggcagcc agcggccccc     1860

cgaccccgct ccaactcccc tgggaaccag cggcctgcct aatgggctgt ccggagatga     1920

agacttctca agcatcgctg atatggactt tagtgccctg ctgtcacaga tttcctctag     1980

tgggcaggga ggaggtggaa gcggcttcag cgtggacacc agtgccctgc tggacctgtt     2040

cagcccctcg gtgaccgtgc ccgacatgag cctgcctgac cttgacagca gcctggccag     2100

tatccaagag ctcctgtctc cccaggagcc ccccaggcct cccgaggcag agaacagcag     2160

cccggattca gggaagcagc tggtgcacta cacagcgcag ccgctgttcc tgctggaccc     2220

cggctccgtg gacaccggga gcaacgacct gccggtgctg tttgagctgg gagagggctc     2280

ctacttctcc gaaggggacg gcttcgccga ggaccccacc atctccctgc tgacaggctc     2340

ggagcctccc aaagccaagg accccactgt ctcctgtaca tccgggtttg ccaacgaact     2400

aggcccgcga cttatgggta aataaaataa aggaaattta ttttcattgc aatagtgtgt     2460

tggttttttg tgtggtcgac gagggcctat ttcccatgat tccttcatat ttgcatatac     2520

gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag     2580

tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat     2640

gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt     2700

tatatatctt gtggaaagga cgaaacaccg gagcaaagga gtcgccttgg ttttagagct     2760

aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata aggctagtcc     2820

gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg gcaccgagtc     2880

ggtgcttttt ttcggtgctt ttttttgaac gctgacgtca tcaacccgct ccaaggaatc     2940

gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg     3000

ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa     3060

atcaccataa acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca     3120

ctctttccca gaatgcatcc aagagtgaac cgttttagag ctaggccaac atgaggatca     3180

cccatgtctg cagggcctag caagttaaaa taaggctagt ccgttatcaa cttggccaac     3240

atgaggatca cccatgtctg cagggccaag tggcaccgag tcggtgcttt ttttcggtgc     3300

ttttttttga tccgacgccg ccatctctag gcccgcgccg gccccctcgc acagacttgt     3360

gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt cctagtaact     3420

atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac tagctacatt     3480

ttacatgata ggcttggatt tctataagag atacaaatac taaattatta ttttaaaaaa     3540

cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag actataaata     3600

tcccttggag aaaagccttg tttggcggtg ggtcattgtt tccggtttta gagctaggcc     3660

aacatgagga tcacccatgt ctgcagggcc tagcaagtta aaataaggct agtccgttat     3720

caacttggcc aacatgagga tcacccatgt ctgcagggcc aagtggcacc gagtcggtgc     3780

tttttttcgg tgcttttttt tctgcagtat ttagcatgcc ccacccatct gcaaggcatt     3840

ctggatagtg tcaaaacagc cggaaatcaa gtccgtttat ctcaaacttt agcattttgg     3900

gaataaatga tatttgctat gctggttaaa ttagatttta gttaaatttc ctgctgaagc     3960

tctagtacga taagcaactt gacctaagtg taaagttgag acttccttca ggtttatata     4020

gcttgtgcgc cgcttgggta cctcggtgcc agtgacgccg gcctgggttt tagagctagg     4080

ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg ctagtccgtt     4140

atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt     4200

gctttttttg agctgtactg agacgttaat taacgtgcag cggccgcagg aacccctagt     4260

gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa     4320

ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcag        4377


<210>  26
<211>  3255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA construct AAV-flexGFP

<400>  26
gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg       60

tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag      120

gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag      180

gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac      240

tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca      300

agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg      360

gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt      420

agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc      480

ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat      540

gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg      600

cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc      660

ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg      720

agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc      780

cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc      840

cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa      900

gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg      960

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc     1020

ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg     1080

ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccccataac     1140

ttcgtataaa gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc     1200

gacccgaatt gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa     1260

gttatactag ggatcccccc tcgagtttta cttgtacagc tcgtccatgc cgagagtgat     1320

cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt     1380

gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc     1440

gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg     1500

gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt     1560

gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc     1620

gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg     1680

ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg     1740

catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg     1800

cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt     1860

gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac     1920

gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt     1980

gaacagctcc tcgcccttgc tcaccatggt ggcggtaccg aattctagta taacttcgta     2040

taggatactt tatacgaagt tatcattggg attcttccta ttttgatcca agcatcacca     2100

tcgaccctct agtccagatc tcaccatcga cccataactt cgtatagcat acattatacg     2160

aagttatgtc cctcgaagag gttcgaattc gatatcaagc ttatcgataa tcaacctctg     2220

gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta     2280

tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt     2340

ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc     2400

aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt     2460

gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg     2520

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac     2580

aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc     2640

acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac     2700

cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct     2760

cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg     2820

ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg     2880

cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt     2940

ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag     3000

ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat     3060

aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta     3120

caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga     3180

ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga     3240

gcgagcgcgc agctg                                                      3255


<210>  27
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes

<400>  27

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ascl1-1

<400>  28
gggagccgct cgctgcagca gcg                                               23


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ascl1-2

<400>  29
ggggctgaat ggagagtttg ca                                                22


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lmx1a-1

<400>  30
gggagcaaag gagtcgcctt g                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lmx1a-2

<400>  31
gaatgcatcc aagagtgaac c                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a2-1

<400>  32
ggcggtgggt cattgtttcc g                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a2-2

<400>  33
gtgccagtga cgccggcctg g                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NeuroD1-1

<400>  34
ggttctggga ggggtgaatg a                                                 21


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NeuroD1-2

<400>  35
ggccatatgg cgcatgccgg gg                                                22


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neurog2-1

<400>  36
ataagctggg gagggagagc                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neurog2-2

<400>  37
aaacaatcag atctgccccg                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PITX3-1

<400>  38
attcacttta tggcaaccca                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PITX3-2

<400>  39
gctagcctgg gagagcccag                                                   20


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FoxA2-1

<400>  40
gaaagtaacc ttgaaacacc g                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FoxA2-2

<400>  41
gggtagccag aaagaggact g                                                 21


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MyoD1-1

<400>  42
ccaataggag tgtagtaggg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MyoD1-2

<400>  43
gagagactgg cagccatacg                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pou5f1-1

<400>  44
atctgcctgt gtcttccaga                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pou5f1-2

<400>  45
tgtccggtga cccaaggcag                                                   20


