                         SEQUENCE LISTING

<110>  H. Lee Moffitt Cancer Center and Research Institute, Inc.
 
<120>  CUSTOMIZED CHIMERIC ANTIGEN RECEPTOR POLYPEPTIDES

<130>  320803-2610

<150>  US 63/149,472
<151>  2021-02-15

<150>  US 63/234,014
<151>  2021-08-17

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val 
            20      


<210>  2
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Pro Tyr Tyr Lys 
            20              


<210>  3
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
1               5                   10                  15      


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            20                  25                  


<210>  4
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
1               5                   10                  15      


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            20                  25                  


<210>  5
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
1               5                   10                  15      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            20                  25                  


<210>  6
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
        35                  40                  45              


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
    50                  55                  60                  


Leu Asp Lys Arg Arg Pro Tyr Tyr Lys 
65                  70              


<210>  7
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Pro Gln Arg Arg Lys Asn Pro Gln Glu 
        35                  40                  45              


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
    50                  55                  60                  


Glu Ile Gly Met Arg Pro Tyr Tyr Lys 
65                  70              


<210>  8
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Glu Arg Arg Arg Gly Lys Gly His Asp 
        35                  40                  45              


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    50                  55                  60                  


Leu His Met Gln Arg Pro Tyr Tyr Lys 
65                  70              


<210>  9
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  9

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
        35                  40                  45              


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
    50                  55                  60                  


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln 
65                  70                  75                  80  


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                85                  90                  95      


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Arg Pro Tyr Tyr Lys 
            100                 105                 110         


<210>  10
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Pro Gln Arg Arg Lys Asn Pro Gln Glu 
        35                  40                  45              


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
    50                  55                  60                  


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
65                  70                  75                  80  


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
                85                  90                  95      


His Met Gln Arg Pro Tyr Tyr Lys 
            100                 


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
        35                  40                  45              


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
    50                  55                  60                  


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Arg Pro Tyr Tyr Lys 
            100                 105                 110         


<210>  12
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
        35                  40                  45              


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
    50                  55                  60                  


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln 
65                  70                  75                  80  


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                85                  90                  95      


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            100                 105                 110         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        115                 120                 125             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Arg Pro Tyr Tyr Lys 
    130                 135                 140             


<210>  13
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln 
1               5                   10                  15      


Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln 
            20                  25                  


<210>  14
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                85                  90                  95      


Arg Arg Pro Tyr Tyr Lys 
            100         


<210>  15
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
                85                  90                  95      


Met Arg Pro Tyr Tyr Lys 
            100         


<210>  16
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
65                  70                  75                  80  


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
                85                  90                  95      


Gln Arg Pro Tyr Tyr Lys 
            100         


<210>  17
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                85                  90                  95      


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
            100                 105                 110         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        115                 120                 125             


Glu Ala Tyr Ser Glu Ile Gly Met Arg Pro Tyr Tyr Lys 
    130                 135                 140     


<210>  18
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
                85                  90                  95      


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
            100                 105                 110         


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
        115                 120                 125             


Arg Pro Tyr Tyr Lys 
    130             


<210>  19
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        35                  40                  45              


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    50                  55                  60                  


Leu Asn Thr Gln Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                85                  90                  95      


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Glu Arg Arg Arg Gly 
            100                 105                 110         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        115                 120                 125             


Thr Tyr Asp Ala Leu His Met Gln Arg Pro Tyr Tyr Lys 
    130                 135                 140     


<210>  20
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20

Gly Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu 
1               5                   10                  15      


Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            20                  25                  30          


Gly Ala Ala Glu Ala Ala Thr Ala Arg Lys Gln Arg Ile Thr Glu Thr 
        35                  40                  45              


Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser 
    50                  55                  60                  


Asp Leu Asn Thr Gln Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
65                  70                  75                  80  


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                85                  90                  95      


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
            100                 105                 110         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        115                 120                 125             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    130                 135                 140                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
145                 150                 155                 160 


Tyr Asp Ala Leu His Met Gln Arg Pro Tyr Tyr Lys 
                165                 170         


