                         SEQUENCE LISTING

<110>  Nanyang Technological University
 
<120>  METHODS FOR (POLY)PEPTIDE TANDEM LIGATION AND CYCLIZATION

<130>  P121987

<150>  SG10202101440P
<151>  2021-02-10

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  269
<212>  PRT
<213>  Artificial

<220>
<223>  VyPAL2 core domain

<400>  1

Asp Ser Ile Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Gly 
1               5                   10                  15      


Tyr His Asn Tyr Arg His Gln Ala Asp Val Cys His Met Tyr Gln Ile 
            20                  25                  30          


Leu Arg Lys Gly Gly Val Lys Asp Glu Asn Ile Ile Val Phe Met Tyr 
        35                  40                  45              


Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Phe Pro Gly Ile Ile Ile 
    50                  55                  60                  


Asn Lys Pro Gly Gly Glu Asn Val Tyr Lys Gly Val Pro Lys Asp Tyr 
65                  70                  75                  80  


Thr Gly Glu Asp Ile Asn Asn Val Asn Phe Leu Ala Ala Ile Leu Gly 
                85                  90                  95      


Asn Lys Ser Ala Ile Ile Gly Gly Ser Gly Lys Val Leu Asp Thr Ser 
            100                 105                 110         


Pro Asn Asp His Ile Phe Ile Tyr Tyr Ala Asp His Gly Ala Pro Gly 
        115                 120                 125             


Lys Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Asp Leu Val 
    130                 135                 140                 


Asp Thr Leu Lys Gln Lys Ala Ala Thr Gly Thr Tyr Lys Ser Met Val 
145                 150                 155                 160 


Phe Tyr Val Glu Ala Cys Asn Ala Gly Ser Met Phe Glu Gly Leu Leu 
                165                 170                 175     


Pro Glu Gly Thr Asn Ile Tyr Ala Met Ala Ala Ser Asn Ser Thr Glu 
            180                 185                 190         


Gly Ser Trp Ile Thr Tyr Cys Pro Gly Thr Pro Asp Phe Pro Pro Glu 
        195                 200                 205             


Phe Asp Val Cys Leu Gly Asp Leu Trp Ser Ile Thr Phe Leu Glu Asp 
    210                 215                 220                 


Cys Asp Ala His Asn Leu Arg Thr Glu Thr Val His Gln Gln Phe Glu 
225                 230                 235                 240 


Leu Val Lys Lys Lys Ile Ala Tyr Ala Ser Thr Val Ser Gln Tyr Gly 
                245                 250                 255     


Asp Ile Pro Ile Ser Lys Asp Ser Leu Ser Val Tyr Met 
            260                 265                 


<210>  2
<211>  281
<212>  PRT
<213>  Artificial

<220>
<223>  butelase-1 active fragment

<400>  2

Val Glu Gly Thr Arg Trp Ala Val Leu Val Ala Gly Ser Lys Gly Tyr 
1               5                   10                  15      


Val Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Leu 
            20                  25                  30          


Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp 
        35                  40                  45              


Asp Ile Ala Tyr Asn Glu Ser Asn Pro His Pro Gly Val Ile Ile Asn 
    50                  55                  60                  


His Pro Tyr Gly Ser Asp Val Tyr Lys Gly Val Pro Lys Asp Tyr Val 
65                  70                  75                  80  


Gly Glu Asp Ile Asn Pro Pro Asn Phe Tyr Ala Val Leu Leu Ala Asn 
                85                  90                  95      


Lys Ser Ala Leu Thr Gly Thr Gly Ser Gly Lys Val Leu Asp Ser Gly 
            100                 105                 110         


Pro Asn Asp His Val Phe Ile Tyr Tyr Thr Asp His Gly Gly Ala Gly 
        115                 120                 125             


Val Leu Gly Met Pro Ser Lys Pro Tyr Ile Ala Ala Ser Asp Leu Asn 
    130                 135                 140                 


Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Ile Val 
145                 150                 155                 160 


Phe Tyr Val Glu Ser Cys Glu Ser Gly Ser Met Phe Asp Gly Leu Leu 
                165                 170                 175     


Pro Glu Asp His Asn Ile Tyr Val Met Gly Ala Ser Asp Thr Gly Glu 
            180                 185                 190         


Ser Ser Trp Val Thr Tyr Cys Pro Leu Gln His Pro Ser Pro Pro Pro 
        195                 200                 205             


Glu Tyr Asp Val Cys Val Gly Asp Leu Phe Ser Val Ala Trp Leu Glu 
    210                 215                 220                 


Asp Cys Asp Val His Asn Leu Gln Thr Glu Thr Phe Gln Gln Gln Tyr 
225                 230                 235                 240 


Glu Val Val Lys Asn Lys Thr Ile Val Ala Leu Ile Glu Asp Gly Thr 
                245                 250                 255     


His Val Val Gln Tyr Gly Asp Val Gly Leu Ser Lys Gln Thr Leu Phe 
            260                 265                 270         


Val Tyr Met Gly Thr Asp Pro Ala Asn 
        275                 280     


<210>  3
<211>  483
<212>  PRT
<213>  Viola yedoensis

<400>  3

Met Gln Leu Phe Ala Ala Gly Val Ile Leu Phe Phe Leu Leu Ala Leu 
1               5                   10                  15      


Ser Gly Thr Ile Ala Gly Gly Leu Asp Val Asp Ser Leu Gln Leu Pro 
            20                  25                  30          


Ser Glu Ala Ala Lys Phe Phe His Asn Asp Asn Ser Thr Asn Asp Asp 
        35                  40                  45              


Asp Ser Ile Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Gly 
    50                  55                  60                  


Tyr His Asn Tyr Arg His Gln Ala Asp Val Cys His Met Tyr Gln Ile 
65                  70                  75                  80  


Leu Arg Lys Gly Gly Val Lys Asp Glu Asn Ile Ile Val Phe Met Tyr 
                85                  90                  95      


Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Phe Pro Gly Ile Ile Ile 
            100                 105                 110         


Asn Lys Pro Gly Gly Glu Asn Val Tyr Lys Gly Val Pro Lys Asp Tyr 
        115                 120                 125             


Thr Gly Glu Asp Ile Asn Asn Val Asn Phe Leu Ala Ala Ile Leu Gly 
    130                 135                 140                 


Asn Lys Ser Ala Ile Ile Gly Gly Ser Gly Lys Val Leu Asp Thr Ser 
145                 150                 155                 160 


Pro Asn Asp His Ile Phe Ile Tyr Tyr Ala Asp His Gly Ala Pro Gly 
                165                 170                 175     


Lys Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Asp Leu Val 
            180                 185                 190         


Asp Thr Leu Lys Gln Lys Ala Ala Thr Gly Thr Tyr Lys Ser Met Val 
        195                 200                 205             


Phe Tyr Val Glu Ala Cys Asn Ala Gly Ser Met Phe Glu Gly Leu Leu 
    210                 215                 220                 


Pro Glu Gly Thr Asn Ile Tyr Ala Met Ala Ala Ser Asn Ser Thr Glu 
225                 230                 235                 240 


Gly Ser Trp Ile Thr Tyr Cys Pro Gly Thr Pro Asp Phe Pro Pro Glu 
                245                 250                 255     


Phe Asp Val Cys Leu Gly Asp Leu Trp Ser Ile Thr Phe Leu Glu Asp 
            260                 265                 270         


Cys Asp Ala His Asn Leu Arg Thr Glu Thr Val His Gln Gln Phe Glu 
        275                 280                 285             


Leu Val Lys Lys Lys Ile Ala Tyr Ala Ser Thr Val Ser Gln Tyr Gly 
    290                 295                 300                 


Asp Ile Pro Ile Ser Lys Asp Ser Leu Ser Val Tyr Met Gly Thr Asp 
305                 310                 315                 320 


Pro Ala Asn Asp Asn Arg Thr Phe Val Asp Glu Asn Ser Leu Arg Pro 
                325                 330                 335     


Pro Leu Lys Val Ile His Gln His Asp Ala Asp Leu Tyr His Ile Trp 
            340                 345                 350         


Cys Lys Tyr Asn Met Ala Pro Glu Gly Ser Ser Lys Lys Ile Glu Ala 
        355                 360                 365             


Gln Lys Gln Leu Leu Glu Leu Met Ser His Arg Ala His Val Asp Asn 
    370                 375                 380                 


Ser Ile Thr Leu Ile Gly Lys Leu Leu Phe Gly Val Asn Lys Ala Ser 
385                 390                 395                 400 


Lys Val Leu Asn Thr Val Arg Pro Val Gly Gln Pro Leu Val Asp Asp 
                405                 410                 415     


Trp Gln Cys Leu Lys Ala Met Ile Arg Thr Phe Glu Thr His Cys Gly 
            420                 425                 430         


Ser Leu Ser Glu Tyr Gly Met Lys His Thr Leu Ser Phe Ala Asn Met 
        435                 440                 445             


Cys Asn Ala Gly Ile Gln Lys Glu Gln Leu Ala Glu Ala Ala Ala Gln 
    450                 455                 460                 


Ala Cys Val Thr Phe Pro Ser Asn Pro Tyr Ser Ser Leu Ala Glu Gly 
465                 470                 475                 480 


Phe Ser Ala 
            


<210>  4
<211>  482
<212>  PRT
<213>  Clitoria ternatea

<400>  4

Met Lys Asn Pro Leu Ala Ile Leu Phe Leu Ile Ala Thr Val Val Ala 
1               5                   10                  15      


Val Val Ser Gly Ile Arg Asp Asp Phe Leu Arg Leu Pro Ser Gln Ala 
            20                  25                  30          


Ser Lys Phe Phe Gln Ala Asp Asp Asn Val Glu Gly Thr Arg Trp Ala 
        35                  40                  45              


Val Leu Val Ala Gly Ser Lys Gly Tyr Val Asn Tyr Arg His Gln Ala 
    50                  55                  60                  


Asp Val Cys His Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp 
65                  70                  75                  80  


Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser 
                85                  90                  95      


Asn Pro His Pro Gly Val Ile Ile Asn His Pro Tyr Gly Ser Asp Val 
            100                 105                 110         


Tyr Lys Gly Val Pro Lys Asp Tyr Val Gly Glu Asp Ile Asn Pro Pro 
        115                 120                 125             


Asn Phe Tyr Ala Val Leu Leu Ala Asn Lys Ser Ala Leu Thr Gly Thr 
    130                 135                 140                 


Gly Ser Gly Lys Val Leu Asp Ser Gly Pro Asn Asp His Val Phe Ile 
145                 150                 155                 160 


Tyr Tyr Thr Asp His Gly Gly Ala Gly Val Leu Gly Met Pro Ser Lys 
                165                 170                 175     


Pro Tyr Ile Ala Ala Ser Asp Leu Asn Asp Val Leu Lys Lys Lys His 
            180                 185                 190         


Ala Ser Gly Thr Tyr Lys Ser Ile Val Phe Tyr Val Glu Ser Cys Glu 
        195                 200                 205             


Ser Gly Ser Met Phe Asp Gly Leu Leu Pro Glu Asp His Asn Ile Tyr 
    210                 215                 220                 


Val Met Gly Ala Ser Asp Thr Gly Glu Ser Ser Trp Val Thr Tyr Cys 
225                 230                 235                 240 


Pro Leu Gln His Pro Ser Pro Pro Pro Glu Tyr Asp Val Cys Val Gly 
                245                 250                 255     


Asp Leu Phe Ser Val Ala Trp Leu Glu Asp Cys Asp Val His Asn Leu 
            260                 265                 270         


Gln Thr Glu Thr Phe Gln Gln Gln Tyr Glu Val Val Lys Asn Lys Thr 
        275                 280                 285             


Ile Val Ala Leu Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly Asp 
    290                 295                 300                 


Val Gly Leu Ser Lys Gln Thr Leu Phe Val Tyr Met Gly Thr Asp Pro 
305                 310                 315                 320 


Ala Asn Asp Asn Asn Thr Phe Thr Asp Lys Asn Ser Leu Gly Thr Pro 
                325                 330                 335     


Arg Lys Ala Val Ser Gln Arg Asp Ala Asp Leu Ile His Tyr Trp Glu 
            340                 345                 350         


Lys Tyr Arg Arg Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Lys 
        355                 360                 365             


Lys Gln Leu Arg Glu Val Met Ala His Arg Met His Ile Asp Asn Ser 
    370                 375                 380                 


Val Lys His Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly His Lys 
385                 390                 395                 400 


Met Leu Asn Asn Val Arg Pro Ala Gly Leu Pro Val Val Asp Asp Trp 
                405                 410                 415     


Asp Cys Phe Lys Thr Leu Ile Arg Thr Phe Glu Thr His Cys Gly Ser 
            420                 425                 430         


Leu Ser Glu Tyr Gly Met Lys His Met Arg Ser Phe Ala Asn Leu Cys 
        435                 440                 445             


Asn Ala Gly Ile Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala 
    450                 455                 460                 


Cys Val Ser Ile Pro Asp Asn Pro Trp Ser Ser Leu His Ala Gly Phe 
465                 470                 475                 480 


Ser Val 
        


<210>  5
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  MLA motif

<400>  5

Lys Lys Ile Ala Tyr Ala 
1               5       


<210>  6
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  MLA motif

<400>  6

Asn Lys Ile Ala Tyr Ala 
1               5       


<210>  7
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  7

Lys Lys Leu Ala Val Ile Asn His Val 
1               5                   


<210>  8
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  8

Gly Ile Gly Gly Ile Lys Ala 
1               5           


<210>  9
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  9

Tyr Lys Ala Asn Gly Leu 
1               5       


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  10

Gly Phe Gly Gly Ile Lys Ala 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  11

Lys Lys Leu Ala Val Ile Asn Gly Phe 
1               5                   


<210>  12
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  12

Gly Arg Ala Asn Gly Ile 
1               5       


<210>  13
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide


<220>
<221>  MISC_FEATURE
<222>  (9)..(22)
<223>  D-amino acid

<400>  13

Gly Ile Gly Gly Phe Lys Gly Gly Lys Leu Ala Lys Leu Ala Lys Lys 
1               5                   10                  15      


Leu Ala Lys Leu Ala Lys 
            20          


<210>  14
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  modified with aminooxy functional group

<400>  14

Gly Phe Leu Gly Val Lys Ala Asn His Val 
1               5                   10  


<210>  15
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  15

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Gly Leu 
1               5                   10                  15      


<210>  16
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  16

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Asp Leu 
1               5                   10                  15      


<210>  17
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  17

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Ala Leu 
1               5                   10                  15      


<210>  18
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  18

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Leu Leu 
1               5                   10                  15      


<210>  19
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  19

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Ser Leu 
1               5                   10                  15      


<210>  20
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  20

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Arg Leu 
1               5                   10                  15      


<210>  21
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  21

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Lys Leu 
1               5                   10                  15      


<210>  22
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  22

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Gln Leu 
1               5                   10                  15      


<210>  23
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  23

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asp Glu Leu 
1               5                   10                  15      


<210>  24
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  24

Gly Ile Ser Thr Lys Ser Ile Pro Pro Ile Ser Tyr Arg Asn Gly Leu 
1               5                   10                  15      


<210>  25
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  25

Gly Ile Gly Gly Ile Arg Lys 
1               5           


<210>  26
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  26

Gln Arg Leu Gly Asn Gln Trp Ala Val Gly His Leu Met Gly Ser Gly 
1               5                   10                  15      


Ser Asp Ser Leu 
            20  


<210>  27
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  27

Ile His Gly His His Ile Ile Ser Val Gly Gly Ser Gly Ser Asp Ser 
1               5                   10                  15      


Leu 
    


<210>  28
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  28

Val Pro Trp Met Glu Pro Ala Tyr Gln Arg Phe Leu Gly Ser Gly Ser 
1               5                   10                  15      


Asp Ser Leu 
            


<210>  29
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  29

Tyr His Trp Tyr Gly Tyr Thr Pro Gln Lys Val Ile Gly Ser Gly Ser 
1               5                   10                  15      


Asp Ser Leu 
            


<210>  30
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  30

Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Gly Ser Gly Ser 
1               5                   10                  15      


Asp Ser Leu 
            


<210>  31
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  31

Cys Arg Gly Asp Arg Gly Asp Cys Gly Ser Gly Ser Asp Ser Leu 
1               5                   10                  15  


<210>  32
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  32

Gly Arg Ala Asp Gly Ile 
1               5       


<210>  33
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  33

Gly Ile Ala Ala Lys 
1               5   


<210>  34
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  34

Tyr Lys Ala Asn Gly Leu 
1               5       


<210>  35
<211>  80
<212>  PRT
<213>  Artificial

<220>
<223>  ZEGFR

<400>  35

Gly Phe Gly Ser Ser Leu Gln Val Asp Asn Lys Phe Asn Lys Glu Met 
1               5                   10                  15      


Trp Ala Ala Trp Glu Glu Ile Arg Asn Leu Pro Asn Leu Asn Gly Trp 
            20                  25                  30          


Gln Met Thr Ala Phe Ile Ala Ser Leu Val Asp Asp Pro Ser Gln Ser 
        35                  40                  45              


Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro 
    50                  55                  60                  


Lys Val Asp Gly Ser Gly Ser Asn His Val His His His His His His 
65                  70                  75                  80  


<210>  36
<211>  90
<212>  PRT
<213>  Artificial

<220>
<223>  Ubiquitin tagged

<400>  36

Gly Gly Ser Gly Ser Gly Ser Gln Ile Phe Val Lys Thr Leu Thr Gly 
1               5                   10                  15      


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
            20                  25                  30          


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
        35                  40                  45              


Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
    50                  55                  60                  


Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg 
65                  70                  75                  80  


Gly Gly Asn Gly Ile Gly Gly Ile Arg Lys 
                85                  90  


<210>  37
<211>  76
<212>  PRT
<213>  Artificial

<220>
<223>  Affibody

<400>  37

Gly Arg Ala Asn Gly Phe Gly Ser Ser Leu Gln Val Asp Asn Lys Phe 
1               5                   10                  15      


Asn Lys Glu Met Trp Ala Ala Trp Glu Glu Ile Arg Asn Leu Pro Asn 
            20                  25                  30          


Leu Asn Gly Trp Gln Met Thr Ala Phe Ile Ala Ser Leu Val Asp Asp 
        35                  40                  45              


Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp 
    50                  55                  60                  


Ala Gln Ala Pro Lys Val Asp Gly Ser Gly Ser Asn 
65                  70                  75      


<210>  38
<211>  78
<212>  PRT
<213>  Artificial

<220>
<223>  ZEGFR

<400>  38

Gly Ser Ser His His His His His His Leu Gln Val Asp Asn Lys Phe 
1               5                   10                  15      


Asn Lys Glu Met Trp Ala Ala Trp Glu Glu Ile Arg Asn Leu Pro Asn 
            20                  25                  30          


Leu Asn Gly Trp Gln Met Thr Ala Phe Ile Ala Ser Leu Val Asp Asp 
        35                  40                  45              


Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp 
    50                  55                  60                  


Ala Gln Ala Pro Lys Val Asp Gly Ser Gly Ser Asn Gly Leu 
65                  70                  75              


<210>  39
<211>  244
<212>  PRT
<213>  Artificial

<220>
<223>  sfGFP-DSL-His6x

<400>  39

Gly Ile Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15      


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg 
            20                  25                  30          


Gly Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe 
        35                  40                  45              


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
    50                  55                  60                  


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
65                  70                  75                  80  


Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
                85                  90                  95      


Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala 
            100                 105                 110         


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
        115                 120                 125             


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
    130                 135                 140                 


Tyr Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 
145                 150                 155                 160 


Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly 
                165                 170                 175     


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
            180                 185                 190         


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val 
        195                 200                 205             


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
    210                 215                 220                 


Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Ser Leu His His 
225                 230                 235                 240 


His His His His 
                


<210>  40
<211>  81
<212>  PRT
<213>  Artificial

<220>
<223>  Affibody

<400>  40

Met Lys Lys Gly Ser Ser His His His His His His Leu Gln Val Asp 
1               5                   10                  15      


Asn Lys Phe Asn Lys Glu Met Trp Ala Ala Trp Glu Glu Ile Arg Asn 
            20                  25                  30          


Leu Pro Asn Leu Asn Gly Trp Gln Met Thr Ala Phe Ile Ala Ser Leu 
        35                  40                  45              


Val Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
    50                  55                  60                  


Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Gly Ser Gly Ser Asp Ser 
65                  70                  75                  80  


Leu 
    


<210>  41
<211>  81
<212>  PRT
<213>  Artificial

<220>
<223>  Affibody

<400>  41

Met Lys Lys Gly Ser Ser His His His His His His Leu Gln Val Asp 
1               5                   10                  15      


Asn Lys Phe Asn Lys Glu Met Trp Ala Ala Trp Glu Glu Ile Arg Asn 
            20                  25                  30          


Leu Pro Asn Leu Asn Gly Trp Gln Met Thr Ala Phe Ile Ala Ser Leu 
        35                  40                  45              


Val Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
    50                  55                  60                  


Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Gly Ser Gly Ser Asp Gly 
65                  70                  75                  80  


Leu 
    


<210>  42
<211>  239
<212>  PRT
<213>  Artificial

<220>
<223>  sfGFP-His6x

<400>  42

Gly Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Asn Ser Leu His His His His His His 
225                 230                 235                 


<210>  43
<211>  79
<212>  PRT
<213>  Artificial

<220>
<223>  ZEGFR

<400>  43

Ile Gly Ser Ser His His His His His His Leu Gln Val Asp Asn Lys 
1               5                   10                  15      


Phe Asn Lys Glu Met Trp Ala Ala Trp Glu Glu Ile Arg Asn Leu Pro 
            20                  25                  30          


Asn Leu Asn Gly Trp Gln Met Thr Ala Phe Ile Ala Ser Leu Val Asp 
        35                  40                  45              


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn 
    50                  55                  60                  


Asp Ala Gln Ala Pro Lys Val Asp Gly Ser Gly Ser Asp Ser Leu 
65                  70                  75                  


<210>  44
<211>  299
<212>  PRT
<213>  Artificial

<220>
<223>  OaAEP1b functional fragment

<400>  44

Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Gly Tyr 
1               5                   10                  15      


Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala Tyr Gln Ile Leu 
            20                  25                  30          


Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp 
        35                  40                  45              


Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly Val Ile Ile Asn 
    50                  55                  60                  


Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr 
65                  70                  75                  80  


Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala Ile Leu Gly Asn 
                85                  90                  95      


Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro 
            100                 105                 110         


Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Ala Ala Gly Val 
        115                 120                 125             


Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Glu Leu Asn Asp 
    130                 135                 140                 


Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe 
145                 150                 155                 160 


Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu Gly Ile Leu Pro 
                165                 170                 175     


Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn Thr Thr Glu Ser 
            180                 185                 190         


Ser Trp Ala Tyr Tyr Cys Pro Ala Gln Glu Asn Pro Pro Pro Pro Glu 
        195                 200                 205             


Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala Trp Leu Glu Asp 
    210                 215                 220                 


Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn Gln Gln Tyr His 
225                 230                 235                 240 


His Val Asp Lys Arg Ile Ser His Ala Ser His Ala Thr Gln Tyr Gly 
                245                 250                 255     


Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr Met Gly Ser Asn 
            260                 265                 270         


Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn Ala Leu Thr Pro 
        275                 280                 285             


Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp 
    290                 295                 


