                         SEQUENCE LISTING

<110>  Boehringer Ingelheim RCV GmbH & Co KG
       Lonza Ltd
       Validogen GmbH
 
<120>  SIGNAL PEPTIDES FOR INCREASED PROTEIN SECRETION

<130>  BOE16959PCT

<150>  EP 21 156 986.8
<151>  2021-02-12

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  PRT
<213>  Komagataella phaffii

<400>  1

Met Leu Asn Lys Leu Phe Ile Ala Ile Leu Ile Val Ile Thr Ala Val 
1               5                   10                  15      


Ile Gly 
        


<210>  2
<211>  18
<212>  PRT
<213>  Komagataella phaffii

<400>  2

Met Lys Leu Ile Ser Val Gly Ile Val Thr Thr Leu Leu Thr Leu Ala 
1               5                   10                  15      


Ser Cys 
        


<210>  3
<211>  66
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pro-sequence of alpha-MF including  L23S, D64E

<400>  3

Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val Ser Leu Glu 
    50                  55                  60                  


Lys Arg 
65      


<210>  4
<211>  85
<212>  PRT
<213>  Artificial sequence

<220>
<223>  alpha-MF (pre-pro-sequence)  including  L23S, D64E

<400>  4

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg 
                85  


<210>  5
<211>  567
<212>  PRT
<213>  Komagataella phaffii

<400>  5

Met Glu Ser Pro Leu Gln Ser Thr Tyr Gly Glu Arg Ala Glu Arg Tyr 
1               5                   10                  15      


Leu Asp Ser Ala Asp Ala Phe His Lys Gln Arg His Arg Leu Asn Arg 
            20                  25                  30          


Arg Leu His Lys Leu Arg Lys Ser Leu Asp Ile His Val Thr Asp Thr 
        35                  40                  45              


Lys Asn Tyr Arg Glu Lys Glu Gln Ile Ser Lys Ile Asp Leu Glu Ser 
    50                  55                  60                  


Tyr Asn Arg Asp Lys Arg Tyr Gly Asp Ile Ile Leu Phe Thr Ala Glu 
65                  70                  75                  80  


Arg Asp His Met Tyr Ser Asp Glu Val Lys Glu Ile Met Lys Val His 
                85                  90                  95      


His Ser Lys Ser Arg Glu Lys Phe Ile Val Ser Arg Leu Lys Arg Ser 
            100                 105                 110         


Leu Asp His Gly Arg Lys Leu Leu Ile Leu Val Gly Asp Glu Pro Asp 
        115                 120                 125             


Glu Met Arg Lys Leu Glu Val Phe Val Tyr Val Ala Leu Ile Gln Gly 
    130                 135                 140                 


Lys Leu Ser Ile Ala Asn Lys Asn Trp Thr Asn Ala Gln Tyr Ala Leu 
145                 150                 155                 160 


Ser Val Ala Arg Cys Gly Leu Gln Phe Leu Asp Lys Tyr Gly Thr Glu 
                165                 170                 175     


Thr Gln Thr Asp Leu Tyr Asn Gly Ile Ile Asp Thr His Ile Asp Gln 
            180                 185                 190         


Met Leu Lys Phe Val Ile Tyr Gln Ala Thr Lys Asn Asn Ser Pro Ile 
        195                 200                 205             


Leu Asp Thr Glu Cys Arg His Gln Ile Arg Thr Asp Thr Leu Gly Tyr 
    210                 215                 220                 


Leu Asp Gln Ala Arg Gln Ile Ile Glu Ser Lys Asp Pro Glu Phe Leu 
225                 230                 235                 240 


Asn Val Gly Val Val Glu Thr Gln Leu Ile Trp Trp Asp Tyr Asp Ile 
                245                 250                 255     


Ser Ile His Ser Glu Glu Val Ala Lys Leu Ile Ser Asp Ala Asn Glu 
            260                 265                 270         


Lys Leu Gln Leu Ile Glu Asp Gly Asn Val Ser Ser Tyr Asp Pro Ala 
        275                 280                 285             


Leu Leu Thr Leu Gln Glu Ala Leu Asp Ala His Gln Leu Leu Met Ala 
    290                 295                 300                 


Arg Asn Val Asp Asn Phe Ala Asp Asp Asp Gln Asn Asn His Val Leu 
305                 310                 315                 320 


Leu Ser Tyr Ile Arg Tyr Leu Leu Leu Ile Thr Thr Leu Arg Arg Asp 
                325                 330                 335     


Ile Thr Leu Ile Asp Gln Val Arg Asn Arg Ser Val Val Asn Ser Ser 
            340                 345                 350         


Leu Ala Val Ala Leu Glu Arg Ala Lys Asp Val Gly Arg Ile Phe Asp 
        355                 360                 365             


Asn Ile Val Lys Lys Val Asn Glu Leu Lys Asp Val Pro Gly Val Tyr 
    370                 375                 380                 


Asn Lys Gln Glu Glu Trp Asn Ser Leu Gln Ala Leu Asp Ala Tyr Phe 
385                 390                 395                 400 


Gln Ala Ser Lys Ile Gln His Leu Ala Ser Thr His Leu Leu Phe Asn 
                405                 410                 415     


Arg Ser Lys Glu Ser Leu Ala Leu Leu Ile Lys Ala Lys Ser Leu Val 
            420                 425                 430         


Lys Gly His Thr Ile Ala Gly Glu Tyr Pro Thr Asn Phe Pro Thr Asn 
        435                 440                 445             


Lys Asp Leu Ser Ser Ile Leu Glu Gln Ile Asn Gln Asp Ile Leu Lys 
    450                 455                 460                 


Ala Tyr Val Leu Ala Lys Tyr Lys Gln Glu Ser Ser Leu Gly Gly Val 
465                 470                 475                 480 


Ser Glu Tyr Asp Phe Ile Ala Asp Asn Arg Asn Lys Val Pro Ser Asn 
                485                 490                 495     


Pro Ser Leu His Lys Ile Ala Ser Val Ser Tyr Lys Asn Val Lys Pro 
            500                 505                 510         


Val Asn Val Lys Pro Val Leu Phe Asp Ile Ala Phe Asn Tyr Val Ser 
        515                 520                 525             


Gln Pro Asn Gln Ile Phe Glu Glu Pro Ile Glu Ser Ser Asn Lys Gln 
    530                 535                 540                 


Glu Arg Gln Ala Asp Ser Glu Ser Pro Ser Pro Glu Lys Lys Lys Lys 
545                 550                 555                 560 


Gly Leu Phe Gly Leu Phe Arg 
                565         


<210>  6
<211>  605
<212>  PRT
<213>  Komagataella phaffii

<400>  6

Met Ser Ser Leu Ser Glu Leu Val Ser Glu Leu Ala Ile His Ser Glu 
1               5                   10                  15      


Lys Arg Gln Tyr Lys Glu Ala Tyr Glu Lys Ala Lys Arg Ile Ile Asp 
            20                  25                  30          


Leu Gly His Pro Leu Asp Leu Asp Thr Leu Lys Leu Gly Leu Val Ala 
        35                  40                  45              


Ser Ile Asn Leu Asp Gln Tyr His Asn Ala Gly Arg Leu Ile Ser Lys 
    50                  55                  60                  


Ser Lys Asp His Ile Val Tyr Asp Gly Met Lys Glu Phe Leu Leu Leu 
65                  70                  75                  80  


Ile Gly Tyr Val Tyr Tyr Lys Asn Gly Asp Ser Lys Asn Phe Glu Thr 
                85                  90                  95      


Leu Leu Lys Asp Ser Ala Phe Gln Gly Arg Ala Phe Glu His Leu Lys 
            100                 105                 110         


Ala Gln Tyr Tyr Tyr Lys Ile Gly Glu Asn Glu Lys Ala Leu Lys Ile 
        115                 120                 125             


Tyr Arg Glu Leu Ser Lys Asn Pro Leu Asp Glu Val Val Asp Leu Ser 
    130                 135                 140                 


Val Asn Glu Arg Ala Val Ile Ser Gln Leu Leu Glu Leu Asp Gly Val 
145                 150                 155                 160 


Val Glu Gln Pro Val Ser Arg Pro Ile Asp Asp Ser Tyr Asp Cys Lys 
                165                 170                 175     


Phe Asn Asp Ala Leu Tyr Gln Val Lys Ile Gly Asp Tyr Glu Ser Ala 
            180                 185                 190         


Leu Asp Leu Leu Glu Glu Ala Lys Ala Ile Cys Glu Glu Asn Thr Lys 
        195                 200                 205             


Asp Leu Pro Leu Asp Thr Arg Glu Ala Glu Ile Val Pro Ile Leu Leu 
    210                 215                 220                 


Gln Ile Ala Tyr Val Lys Gln Leu Lys Gly Lys Lys Glu Glu Ser Leu 
225                 230                 235                 240 


Thr Ala Leu Arg Ser Leu Ser Lys Pro Lys Asp Ser Leu Leu Asp Leu 
                245                 250                 255     


Ile Tyr Arg Asn Asn Leu Leu Ser Leu Arg Ile Asp Glu Tyr Gly Arg 
            260                 265                 270         


Asn Asp Thr Asn Phe His Ile Leu Tyr Arg Glu Leu Gly Phe Pro Asn 
        275                 280                 285             


Ser Ile Asp Ile Asn Lys Asp Lys Leu Thr Val Ser Gln Arg Val Ala 
    290                 295                 300                 


Leu Thr Arg Asn Glu Ser Leu Leu Ala Leu Glu Leu Gly Lys Ile Pro 
305                 310                 315                 320 


Ser Gln Ser Asp Leu Lys Leu Phe Tyr Asp Ala Thr Ser Glu Phe Leu 
                325                 330                 335     


Asp Leu Asn Thr Lys Leu Glu Ala Ser Met Ile Tyr Asn Tyr Phe Met 
            340                 345                 350         


Arg Arg Pro Gly Gln Gln Glu Val Pro Asn Ala Leu Leu Thr Ala Gln 
        355                 360                 365             


Leu Ala Ile Asn Val Gly Asn Ile Asn Asn Ala Arg Thr Val Leu Glu 
    370                 375                 380                 


Thr Val Val Ser Asn Asp Glu Lys Asn Leu Leu Glu Pro Ser Ile Val 
385                 390                 395                 400 


Val Ser Leu Tyr Leu Ile Tyr Asp Lys Leu Gln Ser Gly Arg Leu Gln 
                405                 410                 415     


Val Glu Leu Leu Lys Lys Val Ala Asp Leu Leu Leu Glu Ser Glu Ile 
            420                 425                 430         


Ser Ser Thr Gln Gln Arg Lys Phe Phe Lys Asp Ile Ala Phe Lys Thr 
        435                 440                 445             


Leu Asn His Asp Ala Val Leu Ala Asn Arg Leu Phe Glu Lys Leu His 
    450                 455                 460                 


Ser Ile Tyr Pro Asn Asp Glu Leu Val Ser Thr Tyr Leu Asn Ser Ser 
465                 470                 475                 480 


Ser Asn Ala Ser Asn Asn Asn Thr Thr Thr Thr Asn Phe Ser Glu Leu 
                485                 490                 495     


Asp Asp Leu Val Leu Gly Ile Asp Thr Asp Lys Leu Ile Ser Glu Gly 
            500                 505                 510         


Phe Asp Thr Phe Glu Ser Ser Lys Arg Pro Thr Thr Ile Ile Ser Ser 
        515                 520                 525             


Thr Asn Lys Lys Arg Arg Thr Arg Leu Lys Pro Lys His Glu Ala Lys 
    530                 535                 540                 


Glu Lys Tyr Lys Arg Leu Asp Glu Glu Arg Trp Leu Pro Leu Lys Asp 
545                 550                 555                 560 


Arg Ser Tyr Tyr Arg Pro Lys Lys Gly Lys Lys Ile Arg Asn Thr Thr 
                565                 570                 575     


Gln Gly Thr Val Thr Ser Asn Thr Ser Glu Ile Ser Gly Leu Lys Lys 
            580                 585                 590         


Thr Leu Pro Lys Lys Ser Ser Lys Lys Lys Gly Arg Lys 
        595                 600                 605 


<210>  7
<211>  151
<212>  PRT
<213>  Komagataella phaffii

<400>  7

Met Pro Pro Val Lys Ser Leu Asp Ile Phe Phe Asn Arg Thr Glu Lys 
1               5                   10                  15      


Leu Leu Glu Ala Asn Pro Thr Thr Thr Lys Val Ser Ile Lys Leu Gly 
            20                  25                  30          


Val Asn Phe Asn Asp His Glu Asn Pro Gln Ser Lys His Asn Val Ile 
        35                  40                  45              


Thr Val Arg Val Ser Asp Pro Val Ser Gly Ser Asn Phe Lys Phe Lys 
    50                  55                  60                  


Val Thr Asn Lys Thr Asp Met Leu Lys Ile Phe Ser Phe Leu Gly Pro 
65                  70                  75                  80  


His Gly Ile Glu Leu Pro Ile Ser Gly Gln Gln Ser Gln Ile Lys Ser 
                85                  90                  95      


Asn Asp Gln Thr Gln Ser Asp Asn Thr Glu Val Pro Thr Thr Phe His 
            100                 105                 110         


Arg Gly Ala Thr Ser Ile Leu Ala Asn Lys Ala Phe Glu Lys Lys Pro 
        115                 120                 125             


Leu Ile Ile Lys Asp Ser Ser Thr Ala Lys Lys Gly Gly Lys Gly Gly 
    130                 135                 140                 


Lys Lys Lys Gly Lys Lys Phe 
145                 150     


<210>  8
<211>  270
<212>  PRT
<213>  Komagataella phaffii

<400>  8

Met Pro Leu Leu Glu Glu Ile Ser Asp Ala Glu Asp Ile Asp Asn Leu 
1               5                   10                  15      


Glu Met Asp Leu Ala Glu Phe Asp Pro Thr Leu Arg Thr Pro Ile Ala 
            20                  25                  30          


Glu Gln Arg Pro Ala Pro Gln Val Val Arg Ser Gln Asp Ala Glu Ser 
        35                  40                  45              


Gly Gln Thr Pro Leu Val Pro Asn Gln Asp Gln Ile Ser Gln Tyr Ile 
    50                  55                  60                  


Glu Gln Phe Lys Glu Gly Gly Thr Ile Asn Lys Asp Gln Val Ile Arg 
65                  70                  75                  80  


Pro Asp Glu Met Met Glu Lys Glu Met Ala Glu Leu Lys Ser Phe Gln 
                85                  90                  95      


Ile Leu Tyr Pro Cys Tyr Phe Asp Lys Asn Arg Ser Val Lys Glu Gly 
            100                 105                 110         


Arg Arg Cys Gln Lys Glu Tyr Gly Val Glu Asn Pro Leu Ala Lys Thr 
        115                 120                 125             


Ile Leu Asp Ala Cys Arg Tyr Leu Asp Ile Pro Cys Ile Leu Glu Pro 
    130                 135                 140                 


Glu Lys Thr His Pro Gln Asp Phe Gly Asn Pro Gly Arg Val Arg Val 
145                 150                 155                 160 


Ala Ile Lys Glu Ser Gly Lys Tyr Leu Asp Glu Gln Tyr Lys Thr Lys 
                165                 170                 175     


Arg Lys Leu Ile Gln Leu Val Gly Gln Phe Leu Val Glu His Pro Thr 
            180                 185                 190         


Thr Leu Gln Lys Val Gln Glu Leu Pro Gly Pro Pro Glu Leu Gln Gln 
        195                 200                 205             


Gly Gly Tyr Ile Pro Glu Arg Val Pro Arg Val Lys Gly Leu Lys Met 
    210                 215                 220                 


Asn Glu Ile Val Pro Leu His Ser Pro Phe Thr Ile Lys His Pro Ser 
225                 230                 235                 240 


Thr Lys Ser Val Tyr Glu Arg Glu Pro Glu Pro Ala Pro Pro Ala Ala 
                245                 250                 255     


Val Pro Lys Ala Pro Lys Gln Lys Lys Ile Met Val Arg Arg 
            260                 265                 270 


<210>  9
<211>  518
<212>  PRT
<213>  Komagataella phaffii

<400>  9

Met Val Leu Ala Asp Leu Gly Arg Arg Ile Asn Asn Ala Val Gly Asn 
1               5                   10                  15      


Val Thr Lys Ser Asn Val Val Asp Ala Asp Val Ile Ser Asn Met Leu 
            20                  25                  30          


Lys Glu Ile Cys Asn Ala Leu Leu Glu Ser Asp Val Asn Ile Lys Leu 
        35                  40                  45              


Val Ala Gln Leu Arg Glu Lys Ile Arg Lys Gln Ile Asp Ala Glu Asp 
    50                  55                  60                  


Lys Pro Gly Ile Asn Lys Lys Lys Leu Ile Gln Lys Val Val Phe Asp 
65                  70                  75                  80  


Glu Leu Val Lys Leu Val Asp Cys Asn Glu Ala Glu Leu Phe Lys Pro 
                85                  90                  95      


Lys Lys Lys Gln Thr Asn Val Ile Met Met Val Gly Leu Gln Gly Ala 
            100                 105                 110         


Gly Lys Thr Thr Thr Cys Thr Lys Leu Ala Val Tyr Tyr Gln Arg Arg 
        115                 120                 125             


Gly Phe Lys Val Gly Met Val Cys Gly Asp Thr Phe Arg Ala Gly Ala 
    130                 135                 140                 


Phe Asp Gln Leu Lys Gln Asn Ala Thr Lys Ala Lys Ile Pro Tyr Tyr 
145                 150                 155                 160 


Gly Ser Tyr Thr Glu Thr Asp Pro Val Lys Val Thr Phe Asp Gly Val 
                165                 170                 175     


Glu Glu Phe Arg Lys Glu Lys Phe Glu Ile Ile Ile Val Asp Thr Ser 
            180                 185                 190         


Gly Arg His Arg Gln Glu Glu Asp Leu Phe Glu Glu Met Val Gln Ile 
        195                 200                 205             


Gly Lys Ala Ile Lys Pro Asn Gln Thr Ile Met Val Leu Asp Ala Ser 
    210                 215                 220                 


Ile Gly Gln Ser Ala Glu Ser Gln Ser Lys Ala Phe Lys Glu Ser Ser 
225                 230                 235                 240 


Asp Phe Gly Ala Ile Ile Ile Thr Lys Met Asp Ser Asn Ser Lys Gly 
                245                 250                 255     


Gly Gly Ala Leu Ser Ala Ile Ala Ala Thr Asn Thr Pro Val Ala Phe 
            260                 265                 270         


Ile Ala Thr Gly Glu His Ile Gln Asn Phe Glu Lys Phe Ser Gly Arg 
        275                 280                 285             


Gly Phe Ile Ser Lys Leu Leu Gly Ile Gly Asp Ile Glu Gly Leu Met 
    290                 295                 300                 


Glu His Val Gln Ser Met Asn Leu Asp Gln Gly Asp Thr Ile Lys Asn 
305                 310                 315                 320 


Phe Lys Glu Gly Lys Phe Thr Leu Gln Asp Phe Gln Thr Gln Leu Asn 
                325                 330                 335     


Asn Ile Met Lys Met Gly Pro Leu Ser Lys Leu Ala Gln Met Leu Pro 
            340                 345                 350         


Gly Gly Met Gly Gln Leu Met Gly Gln Val Gly Glu Glu Glu Ala Ser 
        355                 360                 365             


Lys Arg Leu Lys Arg Met Ile Tyr Ile Met Asp Ser Met Thr Lys Gln 
    370                 375                 380                 


Glu Leu Ser Ser Asp Gly Arg Leu Phe Ile Asp Gln Pro Ser Arg Met 
385                 390                 395                 400 


Val Arg Val Ala Arg Gly Ser Gly Thr Ser Val Thr Glu Val Glu Leu 
                405                 410                 415     


Val Leu Leu Gln Gln Lys Met Met Ala Arg Met Ala Leu Gln Ser Lys 
            420                 425                 430         


Asn Met Met Ser Gly Ala Gly Gly Pro Ala Gly Met Ala Ser Lys Met 
        435                 440                 445             


Asn Pro Ala Asn Met Arg Arg Ala Met Gln Gln Met Gln Ser Asn Pro 
    450                 455                 460                 


Gly Met Met Asp Asn Met Met Asn Met Phe Gly Gly Ala Gly Gly Ala 
465                 470                 475                 480 


Gly Gly Ala Gly Met Pro Asp Met Gln Glu Met Met Lys Gln Met Ser 
                485                 490                 495     


Ser Gly Gln Met Lys Met Pro Ser Gln Gln Glu Met Met Ser Met Met 
            500                 505                 510         


Lys Gln Phe Gly Met Gly 
        515             


<210>  10
<211>  151
<212>  PRT
<213>  Komagataella phaffii

<400>  10

Met Ser Thr Thr Thr Lys Lys Asn Lys Asn Arg Ile Leu Ile Glu Asn 
1               5                   10                  15      


His Lys Gln Phe Leu Glu Glu Val Ser Lys Thr Ala Thr Leu Ser Val 
            20                  25                  30          


Trp Asn Ser Lys Phe Ser Ile Lys Arg Leu Ser Leu Glu Ala Asp Pro 
        35                  40                  45              


Val Glu Gly Thr Pro Glu Gly Ile Arg Asp Ile Pro Gln Gly Val Glu 
    50                  55                  60                  


Thr Asn Ser Ile Ile Gly Asn Ser Val Glu Asn Asp Ser Lys Ser His 
65                  70                  75                  80  


Pro Ile Leu Phe Arg Tyr Thr Ala Arg His Ala Lys Glu Lys Ile Pro 
                85                  90                  95      


Glu Val Arg Ile Ser Thr Thr Val Asp Ser Glu Gln Leu Ser Thr Phe 
            100                 105                 110         


Trp Arg Asp Tyr Val Asp Ile Leu Lys Gly Ser Ser Gln Leu Lys Leu 
        115                 120                 125             


Gln Ser Glu Thr Lys Lys Val Ser Ser Lys Lys Ser Lys Ala Lys Lys 
    130                 135                 140                 


Lys Arg Gly Lys Gly Ala Trp 
145                 150     


<210>  11
<211>  323
<212>  DNA
<213>  Komagataella phaffii

<400>  11
atgcagcctc tgatgagtcc gtgaggacga aacgagtaag ctcgtcaggc tgttatggcg       60

catccggggg aggtagttac ttgaccttga ttcctaatag cttacaactg aggtgtctcg      120

ttcgatcctg gcggtccgca atattttcca tacgagtaat ctgtggggga aggcgagcaa      180

taagacgtgc caccgcccaa ggggagcaat ccagcaggga acacgtcccg caaggaggcg      240

ggtgagatag catctcgttg gtaatgggct gttggtgaac aaagtttgac tatgtgaacc      300

ggctatttac atttttgctt ttt                                              323


<210>  12
<211>  1707
<212>  DNA
<213>  Komagataella phaffii

<400>  12
atggaatcgc ccttgcaatc tacatacgga gaaagagccg aaaggtattt agatagtgct       60

gatgcttttc ataaacaaag acacagattg aatcgaaggc tgcacaagtt acgtaagagc      120

ttggatattc atgttactga tactaagaac tatagagaga aagagcagat ttccaaaatt      180

gatctagagt cgtacaacag ggataagcga tatggtgaca ttatactgtt cactgcagag      240

agggatcaca tgtatagtga tgaggtcaag gagatcatga aggtccatca tagtaaatcg      300

agagaaaagt ttattgtttc tagattgaag agatcactgg accacggtag aaaattactg      360

atcctagttg gagacgagcc tgatgagatg agaaaattgg aagtatttgt ttatgttgca      420

ttgattcagg gtaaactttc cattgcaaac aagaattgga ccaatgctca gtatgctctc      480

agtgtggcga gatgtgggct ccagtttttg gacaaatatg gtactgaaac acaaactgac      540

ctctataatg gcataattga cactcacata gatcaaatgt tgaaatttgt gatctaccaa      600

gctactaaaa ataacagtcc tattttggat acagagtgca gacatcaaat taggacggac      660

accctagggt atttggatca ggcaaggcaa ataatagaat caaaagatcc cgagtttctg      720

aatgttggag ttgttgaaac tcagttgatt tggtgggact acgatatctc tattcattca      780

gaggaggtag caaagctgat ttcagatgcg aacgaaaagc tgcaacttat cgaggatgga      840

aacgtctcct catatgatcc ggctctacta actcttcaag aagcgctgga tgctcatcag      900

ttgttgatgg ccagaaatgt tgacaacttc gcagacgacg atcaaaacaa tcatgtttta      960

ctgtcgtaca tcagatattt gttacttatc accactttga gaagggacat tactttgata     1020

gaccaagtta gaaacagatc tgtggttaat tcttccctag ctgtggctct ggaacgtgct     1080

aaagacgttg gtagaatttt cgacaatatc gtcaagaaag tcaatgagtt gaaagacgtt     1140

ccaggtgttt acaacaagca agaggagtgg aattcgttgc aggcattgga tgcttatttc     1200

caagcatcca agatccaaca tttggcatct acccaccttt tattcaacag atccaaggaa     1260

tcattggcgt tattaataaa ggcaaagtcc ttggtaaagg ggcacactat cgccggagaa     1320

tatcccacta atttccctac gaataaagat ttgagttcga tcttagaaca aattaatcaa     1380

gacatcctta aggcttatgt tttggccaag tataagcaag agtcctcttt aggtggtgta     1440

tcggagtatg atttcattgc tgacaatcgc aacaaggttc cgtcgaatcc cagtctgcac     1500

aagattgcct ctgtatccta caagaatgtc aaacctgtca atgttaagcc tgtattgttt     1560

gacatagctt tcaactacgt gagtcaacca aaccagatat ttgaggaacc tatcgagagt     1620

tcaaacaagc aagagagaca agcggattct gaatctccgt caccagagaa gaaaaaaaag     1680

ggattgtttg gattgttccg ctagtag                                         1707


<210>  13
<211>  1821
<212>  DNA
<213>  Komagataella phaffii

<400>  13
atgtcgtctc tttcagagct ggtatcggaa ttggcaatcc attctgagaa gaggcagtac       60

aaagaagcat atgagaaagc aaagcgcatt atagatttgg gccaccctct tgaccttgac      120

acattgaagc taggtttggt ggcttcgatc aacctggacc aatatcacaa cgcaggtcgc      180

ctcatatcaa aaagtaagga tcatatcgta tatgatggaa tgaaggaatt cttgctatta      240

attggatatg tgtactacaa gaacggagac tcgaagaatt ttgaaactct actaaaggat      300

tcagctttcc aaggaagagc atttgaacac ctcaaagccc aatactatta caagatcggg      360

gagaacgaaa aggcactcaa gatttaccga gagctatcta agaacccatt ggatgaagtt      420

gtagacttga gtgtcaatga aagagctgtc attagccagc ttttggaatt ggatggtgtc      480

gttgaacagc ctgtatctcg accaatagac gactcatacg attgcaaatt caacgatgcc      540

ctttaccagg taaagattgg tgactatgaa tctgcattgg atctcttgga agaagccaaa      600

gccatatgtg aagaaaatac aaaagatctg cctttggaca cacgagaagc agagattgtt      660

cctattctgt tacaaattgc ttacgtcaaa caacttaagg gtaaaaagga agaatccttg      720

actgcgctga gaagtctttc taaaccaaag gactctcttt tagatcttat ttacagaaat      780

aacttactgt cattaaggat tgatgaatac ggaagaaatg ataccaactt tcatattctt      840

tatcgtgagt taggattccc taattcgata gacattaata aagacaagtt gacagtgtcc      900

caaagggttg cgttgaccag aaatgaatca ttattggcac tcgagcttgg aaagatccca      960

tctcaaagtg atctcaagct cttttatgat gctacttcgg aatttttaga tttgaacacc     1020

aagctagaag cctcaatgat ttataattat ttcatgagac gccctggcca gcaagaggtt     1080

ccaaatgctc ttttaactgc acagctggct atcaacgttg gtaacatcaa taatgcaaga     1140

actgttcttg aaactgtggt gtcgaacgac gaaaagaatt tactagaacc atctattgtg     1200

gtatctttgt atttgattta cgataagctt caaagcggaa gattgcaagt tgaactcctg     1260

aagaaagtag cggatctttt gctagaatca gagatttcca gcactcaaca acgtaagttt     1320

ttcaaagata ttgccttcaa aaccctcaat cacgatgcag ttttggccaa tcgactattt     1380

gagaaactgc atagtattta ccctaatgat gagttggtat ccacgtactt gaactcttca     1440

tctaatgcat ccaacaataa cactaccacg accaacttct ccgaattgga cgacttggtc     1500

ctaggcatag acacggataa gcttattagt gaaggatttg atacttttga gtccagcaaa     1560

agaccgacca cgattatcag ctcaactaac aagaaacgtc gtactagatt gaagcccaaa     1620

catgaagcca aggaaaagta taagcgtctg gatgaggaaa gatggttacc tttaaaggac     1680

cgcagttatt acagacccaa aaagggaaag aaaattagaa ataccactca gggtactgtc     1740

actagtaata ctagtgaaat aagtggcttg aaaaagactc tgccaaagaa aagttccaaa     1800

aagaaaggaa gaaaatgata g                                               1821


<210>  14
<211>  456
<212>  DNA
<213>  Komagataella phaffii

<400>  14
atgcctcctg tgaaatctct ggacatcttt ttcaaccgca cagagaagct cttagaagcc       60

aaccccacaa cgacaaaagt ttccatcaaa ttgggcgtaa atttcaatga tcacgagaat      120

cctcaaagca agcacaacgt cataacggtg agagtatctg atccagtgag cgggtccaat      180

ttcaaattca aagtgaccaa taaaactgat atgctgaaaa tattcagttt cttaggtcct      240

catggcattg agttaccaat ttctggccag caaagccaga taaagagtaa tgatcagact      300

cagagtgaca atactgaagt gcctaccaca tttcataggg gagccaccag tattttggct      360

aataaggcat ttgagaagaa accactgatt attaaggatt caagtaccgc aaagaaaggt      420

ggtaaaggtg gtaagaagaa gggtaagaaa ttttaa                                456


<210>  15
<211>  816
<212>  DNA
<213>  Komagataella phaffii

<400>  15
atgccattac tagaggaaat aagtgatgca gaggacatag acaacttgga gatggattta       60

gccgagtttg atcctacttt aaggactccg atagctgagc aaagaccagc tcctcaggtt      120

gtcagatcac aagatgccga aagtggacag actcctttgg ttcctaacca ggatcaaata      180

agtcagtata ttgaacaatt caaagaaggt ggcaccataa acaaggatca agtgattaga      240

cccgacgaaa tgatggaaaa agaaatggca gagttgaaaa gcttccaaat tttgtaccca      300

tgttactttg ataaaaatag aagtgttaaa gaaggaagaa gatgccaaaa ggagtatggt      360

gtggagaacc ccctggcaaa gacaatatta gatgcttgca ggtacttgga tataccttgc      420

atcctggagc ctgaaaagac tcatcctcaa gattttggta atccaggaag agtgagagtg      480

gctatcaagg agagtgggaa gtatctcgat gaacaatata agaccaaaag gaaactaata      540

cagttggtag gacaatttct ggttgaacat ccaacaacgt tacagaaagt tcaagaattg      600

cccggtccac ctgagttgca acagggcggg tacattccag aacgtgtacc ccgagtgaaa      660

gggttaaaga tgaacgaaat tgttcctttg cattcgccat tcactattaa gcatccaagt      720

actaaatctg tttatgaaag ggaacctgag cccgcaccac ccgccgccgt gcccaaagct      780

ccgaaacaga agaaaataat ggtgagaaga taatag                                816


<210>  16
<211>  1560
<212>  DNA
<213>  Komagataella phaffii

<400>  16
atggtattgg cagatcttgg aaggcgtatc aataacgccg ttggaaatgt caccaagtcc       60

aatgttgttg acgctgacgt catcagcaac atgttaaagg agatttgtaa cgccctattg      120

gagtccgatg tgaacattaa actagttgcc caattgagag agaaaatacg aaaacagatc      180

gacgcagagg ataaaccagg aattaataag aagaagctga tccagaaggt cgtttttgat      240

gagctggtga aacttgttga ttgcaacgaa gctgagctgt tcaagccaaa gaaaaaacag      300

acgaatgtga tcatgatggt cggtttacaa ggtgctggta agacaacaac ctgtactaaa      360

ctggcagtgt attaccagag aagaggattc aaagtgggaa tggtctgtgg tgacactttc      420

cgagctggtg cgtttgacca gctgaaacaa aacgctacca aggctaagat tccctactat      480

ggttcatata cagaaactga ccctgtgaaa gtgacctttg atggtgtgga agaattcagg      540

aaggaaaagt ttgaaataat aattgtggat acttctggta gacacaggca ggaggaagat      600

ttattcgaag agatggtaca aattggaaaa gctatcaagc ctaatcaaac aatcatggta      660

ctggatgctt ccataggtca atctgccgaa tctcaatcta aagcatttaa ggaatcatcc      720

gattttggtg ccattatcat aactaaaatg gattccaatt ccaagggagg aggtgccctt      780

tcagctatag ctgccaccaa cactccagta gcgtttattg ccaccggaga gcacattcag      840

aatttcgaaa agttttcagg aagaggattt atctcaaaac ttttaggaat tggtgatata      900

gagggtctta tggaacatgt tcagtcgatg aacttggatc aaggtgatac tatcaagaat      960

ttcaaggaag gaaagtttac tttacaggat tttcaaacgc aattgaacaa catcatgaag     1020

atggggccac tgtccaaact cgctcaaatg ttgcctggtg gaatgggaca attgatggga     1080

caggttggtg aagaggaggc ttcaaagaga ttgaagcgaa tgatttatat aatggattca     1140

atgacgaagc aagagttgtc aagtgacggt agattgttta ttgatcagcc ttcaaggatg     1200

gtaagagttg ctagaggctc tggtacctct gtaactgagg tggagcttgt tcttttacag     1260

caaaagatga tggctcgtat ggcattacaa tctaagaata tgatgagtgg ggccggcggt     1320

ccagcaggga tggcttccaa aatgaatcca gctaatatga gaagagctat gcaacaaatg     1380

caatcaaacc caggaatgat ggataacatg atgaatatgt ttggtggagc tggaggagct     1440

ggaggagctg gaatgccgga tatgcaagaa atgatgaagc aaatgtccag tggccaaatg     1500

aaaatgccca gtcaacagga aatgatgagc atgatgaaac agtttggtat gggctaatag     1560


<210>  17
<211>  456
<212>  DNA
<213>  Komagataella phaffii

<400>  17
atgtccacaa ctactaagaa aaacaagaac aggatcttga tagagaatca caaacagttc       60

ctggaagaag tttccaaaac agccacttta tcagtttgga actcgaaatt ttcaatcaaa      120

cgactgtctc tagaagcaga tcccgtggaa gggacgcctg aaggaatcag agatatccca      180

caaggagtag agacaaactc tataatagga aacagcgtag agaatgattc aaagtcacac      240

cccattttat tcagatatac agctagacac gcaaaggaga aaataccaga ggtgcgaatt      300

tcaaccaccg ttgattcaga gcagctaagc accttctgga gagattatgt ggacatattg      360

aagggaagct cccaattgaa actgcagtca gaaaccaaga aagtcagtag taagaagagc      420

aaggcgaaga agaagagagg aaagggtgca tggtaa                                456


<210>  18
<211>  54
<212>  DNA
<213>  Komagataella phaffii

<400>  18
atgaagctga tctccgtggg tatagtgacg acattactga ctttggccag ttgc             54


<210>  19
<211>  54
<212>  DNA
<213>  Komagataella phaffii

<400>  19
atgttaaaca agctgttcat tgcaatactc atagtcatca ctgctgtcat aggc             54


<210>  20
<211>  198
<212>  DNA
<213>  Artificial

<220>
<223>  Pro-sequence of alpha-MF including  L23S, D64E

<400>  20
gcccctgtta acactaccac tgaagacgag actgctcaaa ttccagctga agcagttatc       60

ggttactctg accttgaggg tgatttcgac gtcgctgttt tgcctttctc taactccact      120

aacaacggtt tgttgttcat taacaccact atcgcttcca ttgctgctaa ggaagagggt      180

gtctctctcg agaagaga                                                    198


<210>  21
<211>  255
<212>  DNA
<213>  Artificial

<220>
<223>  alpha-MF (pre-pro-sequence)  including  L23S, D64E

<400>  21
atgagattcc catctatttt caccgctgtc ttgttcgctg cctcctctgc attggctgcc       60

cctgttaaca ctaccactga agacgagact gctcaaattc cagctgaagc agttatcggt      120

tactctgacc ttgagggtga tttcgacgtc gctgttttgc ctttctctaa ctccactaac      180

aacggtttgt tgttcattaa caccactatc gcttccattg ctgctaagga agagggtgtc      240

tctctcgaga agaga                                                       255


<210>  22
<211>  837
<212>  DNA
<213>  Artificial

<220>
<223>  vHH protein

<400>  22
caggttcagc tgcaggagtc cggtggtggt ctggttcaag ccggtggttc attaagattg       60

tcctgtgctg cctctggtag aactttcact tctttcgcaa tgggttggtt tagacaagca      120

cctggaaaag agagagagtt tgttgcttct atctccagat ccggtacttt aactagatac      180

gctgactctg ccaagggtag attcactatt tctgttgaca acgccaagaa cactgtttct      240

ttgcaaatgg acaaccttaa cccagatgac accgcagtct attactgtgc cgctgacttg      300

cacagaccat acggtccagg aacccaaaga tccgatgagt acgattcttg gggtcaggga      360

actcaagtca ctgtctcttc aggtggtgga tctggtggtg gaggttcagg tggtggagga      420

tccggtggtg gtggttctgg tggtggtgga tctggtggag gtgaagttca acttgtcgaa      480

tccggtggtg cacttgtcca acctggtgga tctcttagac tttcttgtgc cgcctccggt      540

tttcctgtta accgttactc tatgcgttgg tacagacaag cccctggaaa agaacgtgaa      600

tgggttgccg gaatgtcctc agctggtgac agatcctcct acgaagattc tgtgaaggga      660

cgtttcacca tctccagaga tgacgcccgt aacaccgttt accttcaaat gaactccctt      720

aagcctgagg atactgccgt ctactattgt aacgtgaatg tcggatttga atactgggga      780

cagggaaccc aagttactgt ctcttccggt ggacatcacc accaccatca ctaatag         837


<210>  23
<211>  774
<212>  DNA
<213>  Artificial

<220>
<223>  scR protein

<400>  23
caggaacaac taatggagtc tgggggtggt ttggttaccc tgggtggttc tcttaagctt       60

tcatgtaagg cctctggtat tgatttttcg cactacggta tctcctgggt tagacaagct      120

cctggaaaag gtctggaatg gatcgcttac atttacccaa attacggttc tgttgactat      180

gcctcctggg tcaatggtag gttcactatt tcccttgaca acgctcagaa cacggtattc      240

ctacagatga tctccctaac cgctgctgat actgcaacct acttctgtgc tcgtgacaga      300

ggttactact ctggctctcg tggaactaga cttgacttat ggggacaagg tactctcgtt      360

accatctcta gtggtggagg tggttctgga ggaggaggtt ccggcggagg tggtagcgag      420

ctggtcatga ctcaaacccc tccatcccta tctgcatcag tcggtgaaac cgttagaatt      480

agatgccttg catctgagtt cttgttcaac ggtgtgtcct ggtatcaaca aaagcctggt      540

aagcctccaa agtttctcat ttctggtgcc tcaaacctcg aatctggagt gccaccaaga      600

ttttccggat ctggctctgg tactgactac actctgacaa ttggtggtgt tcaagctgag      660

gatgttgcta cctactattg tctcggtggt tactcaggat cttccggcct aactttcggt      720

gccggtacaa acgtcgagat caaaggtgga catcaccacc accatcacta atag            774


<210>  24
<211>  687
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-HC

<400>  24
gaggtccaat tggtccaatc tggtggagga ttggttcaac caggtggatc tctgagattg       60

tcttgtgctg cttctggttt caccttctct cactactgga tgtcatgggt tagacaagct      120

cctggtaagg gtttggaatg ggttgctaac atcgagcaag atggatcaga gaagtactac      180

gttgactctg ttaagggaag attcactatt tcccgtgata acgccaagaa ctccttgtac      240

ctgcaaatga actcccttag agctgaggat actgctgtct acttctgtgc tagagacttg      300

gaaggtttgc atggtgatgg ttacttcgac ttatggggta gaggtactct tgtcaccgtt      360

tcatctgcct ctaccaaagg accttctgtg ttcccattag ctccatgttc cagatccacc      420

tccgaatcta ctgcagcttt gggttgtttg gtgaaggact actttcctga accagtgact      480

gtctcttgga actctggtgc tttgacttct ggtgttcaca cctttcctgc agttttgcag      540

tcatctggtc tgtactctct gtcctcagtt gtcactgttc cttcctcatc tcttggtacc      600

aagacctaca cttgcaacgt tgaccataag ccatccaata ccaaggttga caagagagtt      660

gagtccaagt atggtccacc ttaatag                                          687


<210>  25
<211>  648
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-LC

<400>  25
gctatccagt tgactcaatc accatcctct ttgtctgctt ctgttggtga tagagtcatc       60

ctgacttgtc gtgcatctca aggtgtttcc tcagctttag cttggtacca acaaaagcca      120

ggtaaagctc caaagttgct gatctacgac gcttcatccc ttgaatctgg tgttccttca      180

cgtttctctg gatctggatc aggtcctgat ttcactctga ctatctcatc ccttcaacca      240

gaggactttg ctacctactt ctgtcaacag ttcaactctt accctttgac ctttggaggt      300

ggaactaagt tggagatcaa gagaactgtt gctgcaccat cagtgttcat ctttcctcca      360

tctgatgagc aactgaagtc tggtactgca tctgttgtct gcttactgaa caacttctac      420

ccaagagaag ctaaggtcca atggaaggtt gacaatgcct tgcaatctgg taactctcaa      480

gagtctgtta ctgagcaaga ctctaaggac tctacttact ccctttcttc caccttgact      540

ttgtctaagg ctgattacga gaagcacaag gtttacgctt gtgaggttac tcaccaaggt      600

ttgtcctctc ctgttaccaa gtctttcaac agaggtgaat gctaatag                   648


<210>  26
<211>  277
<212>  PRT
<213>  Artificial

<220>
<223>  vHH protein

<400>  26

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Thr Ser Phe 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Ser Arg Ser Gly Thr Leu Thr Arg Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Val Asp Asn Ala Lys Asn Thr Val Ser 
65                  70                  75                  80  


Leu Gln Met Asp Asn Leu Asn Pro Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Leu His Arg Pro Tyr Gly Pro Gly Thr Gln Arg Ser Asp 
            100                 105                 110         


Glu Tyr Asp Ser Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Glu Val Gln Leu Val Glu 
145                 150                 155                 160 


Ser Gly Gly Ala Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
                165                 170                 175     


Ala Ala Ser Gly Phe Pro Val Asn Arg Tyr Ser Met Arg Trp Tyr Arg 
            180                 185                 190         


Gln Ala Pro Gly Lys Glu Arg Glu Trp Val Ala Gly Met Ser Ser Ala 
        195                 200                 205             


Gly Asp Arg Ser Ser Tyr Glu Asp Ser Val Lys Gly Arg Phe Thr Ile 
    210                 215                 220                 


Ser Arg Asp Asp Ala Arg Asn Thr Val Tyr Leu Gln Met Asn Ser Leu 
225                 230                 235                 240 


Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Val Asn Val Gly Phe 
                245                 250                 255     


Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly His 
            260                 265                 270         


His His His His His 
        275         


<210>  27
<211>  256
<212>  PRT
<213>  Artificial

<220>
<223>  scR protein

<400>  27

Gln Glu Gln Leu Met Glu Ser Gly Gly Gly Leu Val Thr Leu Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Lys Ala Ser Gly Ile Asp Phe Ser His Tyr 
            20                  25                  30          


Gly Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Tyr Ile Tyr Pro Asn Tyr Gly Ser Val Asp Tyr Ala Ser Trp Val 
    50                  55                  60                  


Asn Gly Arg Phe Thr Ile Ser Leu Asp Asn Ala Gln Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ile Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Arg Gly Tyr Tyr Ser Gly Ser Arg Gly Thr Arg Leu Asp 
            100                 105                 110         


Leu Trp Gly Gln Gly Thr Leu Val Thr Ile Ser Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Val Met Thr 
    130                 135                 140                 


Gln Thr Pro Pro Ser Leu Ser Ala Ser Val Gly Glu Thr Val Arg Ile 
145                 150                 155                 160 


Arg Cys Leu Ala Ser Glu Phe Leu Phe Asn Gly Val Ser Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Lys Pro Pro Lys Phe Leu Ile Ser Gly Ala Ser Asn 
            180                 185                 190         


Leu Glu Ser Gly Val Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Tyr Thr Leu Thr Ile Gly Gly Val Gln Ala Glu Asp Val Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys Leu Gly Gly Tyr Ser Gly Ser Ser Gly Leu Thr Phe Gly 
225                 230                 235                 240 


Ala Gly Thr Asn Val Glu Ile Lys Gly Gly His His His His His His 
                245                 250                 255     


<210>  28
<211>  227
<212>  PRT
<213>  Artificial

<220>
<223>  SDZ-Fab-HC

<400>  28

Glu Val Gln Leu Val Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser His Tyr 
            20                  25                  30          


Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asn Ile Glu Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Leu Glu Gly Leu His Gly Asp Gly Tyr Phe Asp Leu Trp 
            100                 105                 110         


Gly Arg Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
        115                 120                 125             


Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr 
    130                 135                 140                 


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
145                 150                 155                 160 


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
                165                 170                 175     


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
            180                 185                 190         


Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp 
        195                 200                 205             


His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr 
    210                 215                 220                 


Gly Pro Pro 
225         


<210>  29
<211>  214
<212>  PRT
<213>  Artificial

<220>
<223>  SDZ-Fab-LC

<400>  29

Ala Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ile Leu Thr Cys Arg Ala Ser Gln Gly Val Ser Ser Ala 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Pro Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln Phe Asn Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  30
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  SRP14 fwd

<400>  30
agacacgcaa aggagaaaa                                                    19


<210>  31
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  SRP14 rev

<400>  31
gtccacataa tctctccaga ag                                                22


<210>  32
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  SRP21 fwd

<400>  32
ggggagccac cagtatttt                                                    19


<210>  33
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  SRP21 rev

<400>  33
ccacctttac cacctttctt t                                                 21


<210>  34
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Sec65 fwd

<400>  34
ttcctttgca ttcgccatt                                                    19


<210>  35
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  SEC65 rev

<400>  35
ttcttctgtt tcggagcttt g                                                 21


<210>  36
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  SRP54 fwd

<400>  36
aagatgatgg ctcgtatgg                                                    19


<210>  37
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  SRP54 rev

<400>  37
tcctgggttt gattgcattt                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  SRP68 fwd

<400>  38
caactacgtg agtcaaccaa                                                   20


<210>  39
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  SRP68 rev

<400>  39
agcggaacaa tccaaacaa                                                    19


<210>  40
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  SRP72 fwd

<400>  40
tgccttcaaa accctcaatc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  SRP72 rev

<400>  41
ggtcgtggta gtgttattgt                                                   20


<210>  42
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  scpRNA fwd

<400>  42
gggaaggcga gcaataag                                                     18


<210>  43
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  scpRNA rev

<400>  43
accaacagcc cattacca                                                     18


<210>  44
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  scR fwd

<400>  44
aagcctggta agcctccaaa gt                                                22


<210>  45
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  scR rev

<400>  45
tcctcagctt gaacaccacc aat                                               23


<210>  46
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  vHH fwd

<400>  46
tgtaacgtga atgtcggatt tg                                                22


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  vHH rev

<400>  47
tagtgatggt ggtggtgatg                                                   20


<210>  48
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-HC fwd

<400>  48
tactgctgct ttgggttgtt tggt                                              24


<210>  49
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-HC rev

<400>  49
aagggacagt aacaacagag gaca                                              24


<210>  50
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-LC fwd

<400>  50
gatgaacaat tgaagtctgg tac                                               23


<210>  51
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  SDZ-Fab-LC rev

<400>  51
gagtaacttc acaagcgtaa acc                                               23


<210>  52
<211>  18
<212>  PRT
<213>  Komagataella pastoris

<400>  52

Met Lys Leu Phe Phe Val Gly Ile Val Thr Thr Leu Leu Thr Leu Val 
1               5                   10                  15      


Ser Cys 
        


<210>  53
<211>  66
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  53

Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val Ser Leu Asp 
    50                  55                  60                  


Lys Arg 
65      


<210>  54
<211>  741
<212>  DNA
<213>  Artificial

<220>
<223>  mCherry

<400>  54
gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg       60

cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc      120

ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc      180

gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc      240

gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg      300

atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc      360

gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg      420

cagaaaaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc      480

ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag      540

gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac      600

atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc      660

gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggtgg agattacaag      720

gatgacgatg ataagtaata g                                                741


<210>  55
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  mCherry

<400>  55

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Gly Asp Tyr Lys 
225                 230                 235                 240 


Asp Asp Asp Asp Lys 
                245 


<210>  56
<211>  16
<212>  PRT
<213>  Komagataella phaffii

<400>  56

Met Leu Val Ala Trp Phe Leu Leu Leu Leu Val Ser Ser Cys Ile Cys 
1               5                   10                  15      


<210>  57
<211>  19
<212>  PRT
<213>  Komagataella phaffii

<400>  57

Met Lys Phe Ala Ile Ser Thr Leu Leu Ile Ile Leu Gln Ala Ala Ala 
1               5                   10                  15      


Val Phe Ala 
            


<210>  58
<211>  20
<212>  PRT
<213>  Komagataella phaffii

<400>  58

Met Lys Phe Gly Leu Gly Ser Leu Gly Leu Ala Val Ala Leu Ile Pro 
1               5                   10                  15      


Ile Ala Ser Ala 
            20  


<210>  59
<211>  18
<212>  PRT
<213>  Komagataella phaffii

<400>  59

Met Ile Ile Leu Leu Pro Leu Leu Phe Leu Phe Val Ala Gly Leu Val 
1               5                   10                  15      


Gln Ala 
        


<210>  60
<211>  16
<212>  PRT
<213>  Komagataella phaffii

<400>  60

Met Asp Pro Phe Ser Ile Leu Leu Thr Leu Thr Leu Ile Ile Leu Ala 
1               5                   10                  15      


<210>  61
<211>  24
<212>  PRT
<213>  Komagataella phaffii

<400>  61

Met Arg Leu Ser Tyr Glu Cys Leu Phe Ser Val Phe Leu Val Leu Ala 
1               5                   10                  15      


Tyr His Leu Lys Gly Thr Lys Ala 
            20                  


<210>  62
<211>  20
<212>  PRT
<213>  Komagataella phaffii

<400>  62

Met Ile Asn Leu Asn Ser Phe Leu Ile Leu Thr Val Thr Leu Leu Ser 
1               5                   10                  15      


Pro Ala Leu Ala 
            20  


<210>  63
<211>  18
<212>  PRT
<213>  Komagataella phaffii

<400>  63

Met Gln Leu Gln Tyr Leu Ala Val Leu Cys Ala Leu Leu Leu Asn Val 
1               5                   10                  15      


Gln Ser 
        


<210>  64
<211>  20
<212>  PRT
<213>  Komagataella phaffii

<400>  64

Met Trp Ile Glu Arg Asn Leu Ile Ala Ser Ile Leu Leu Phe Ser Thr 
1               5                   10                  15      


Ser Ala Tyr Ala 
            20  


<210>  65
<211>  25
<212>  PRT
<213>  Komagataella phaffii

<400>  65

Met Asn Ile Ser Thr Ala Ser Lys Ile Ser Arg Leu Leu Gln Leu Val 
1               5                   10                  15      


Ile Ala Leu Ile Ser Leu Val Leu Thr 
            20                  25  


<210>  66
<211>  20
<212>  PRT
<213>  Komagataella phaffii

<400>  66

Met Phe Val Phe Glu Pro Val Leu Leu Ala Val Leu Val Ala Ser Thr 
1               5                   10                  15      


Cys Val Thr Ala 
            20  


<210>  67
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  mCherry forward

<400>  67
catcaagttg gacatcacct cc                                                22


<210>  68
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  mCherrey reverse

<400>  68
cacccttgta cagctcgtcc                                                   20


<210>  69
<211>  100
<212>  PRT
<213>  Komagataella phaffii

<400>  69

Leu Leu Ile Pro Ser Leu Asp Gln Leu Asn Ile Gln Leu Pro Phe Ser 
1               5                   10                  15      


Leu Pro His His Thr Glu Ser Pro Ser Leu Lys Leu Gln Gly Ser Asn 
            20                  25                  30          


Pro Phe Glu Ser Ser Thr Val Arg Pro Asp Pro Ile Gln Ile Tyr Ser 
        35                  40                  45              


Thr Gly Tyr Lys Val Ile Glu Asn Ser Tyr Ile Val Thr Val Asp Ser 
    50                  55                  60                  


Ser Ile Thr Asp Ser Glu Leu Gln Gln Leu Tyr Asp Tyr Ile Lys Gly 
65                  70                  75                  80  


Gly Tyr Glu Phe Met Leu Asn Asn Glu Asp Pro Phe Phe Val Ala Met 
                85                  90                  95      


Gly Ile Lys Arg 
            100 


<210>  70
<211>  62
<212>  PRT
<213>  Komagataella phaffii

<400>  70

Cys Leu Gln Leu Leu Thr Thr Ser Ile Pro Pro Ser Phe Leu Thr Met 
1               5                   10                  15      


Val Pro Glu His Tyr Ile Gly Ser Lys Ser Val Asp Glu Val Pro Thr 
            20                  25                  30          


Ser Glu Asp Pro Arg Val Asn Ala Cys Pro Tyr Ile Cys Asp Ile Gly 
        35                  40                  45              


Asp Cys Ser Arg Gly Tyr Ser Arg Ala Ser His Leu Lys Arg 
    50                  55                  60          


<210>  71
<211>  13
<212>  PRT
<213>  Komagataella phaffii

<400>  71

Arg Pro Leu Glu His Ala His His Gln His Asp Lys Arg 
1               5                   10              


<210>  72
<211>  8
<212>  PRT
<213>  Komagataella phaffii

<400>  72

Tyr Pro Leu Val Ile Lys Lys Arg 
1               5               


<210>  73
<211>  2
<212>  PRT
<213>  Komagataella phaffii

<400>  73

Lys Arg 
1       


<210>  74
<211>  66
<212>  PRT
<213>  Saccharomyces paradoxus

<400>  74

Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Asn Ile Ala Ala Glu Glu Glu Gly Val Thr Leu Asn 
    50                  55                  60                  


Lys Arg 
65      


<210>  75
<211>  66
<212>  PRT
<213>  Saccharomyces paradoxus

<400>  75

Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Ile Ala 
            20                  25                  30          


Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Asn Ile Ala Ala Glu Glu Glu Gly Val Thr Leu Asn 
    50                  55                  60                  


Lys Arg 
65      


<210>  76
<211>  65
<212>  PRT
<213>  Saccharomyces paradoxus

<400>  76

Pro Val Asn Thr Thr Thr Glu Asp Glu Met Ala Gln Ile Pro Ala Glu 
1               5                   10                  15      


Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala Val 
            20                  25                  30          


Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn Thr 
        35                  40                  45              


Thr Ile Ala Asn Ile Ala Ala Glu Glu Glu Gly Val Thr Leu Asn Lys 
    50                  55                  60                  


Arg 
65  


<210>  77
<211>  65
<212>  PRT
<213>  Saccharomyces paradoxus

<400>  77

Pro Val Asn Thr Thr Thr Glu Asp Glu Met Ala Gln Ile Pro Ala Glu 
1               5                   10                  15      


Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala Val 
            20                  25                  30          


Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn Thr 
        35                  40                  45              


Thr Ile Ala Asn Ile Ala Ala Glu Glu Glu Gly Val Thr Leu Asn Lys 
    50                  55                  60                  


Arg 
65  


<210>  78
<211>  65
<212>  PRT
<213>  Saccharomyces paradoxus

<400>  78

Pro Val Asn Thr Thr Thr Glu Asp Glu Met Ala Arg Ile Pro Ala Glu 
1               5                   10                  15      


Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala Val 
            20                  25                  30          


Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn Thr 
        35                  40                  45              


Thr Ile Ala Asn Ile Ala Ala Glu Glu Glu Gly Val Thr Leu Asn Lys 
    50                  55                  60                  


Arg 
65  


<210>  79
<211>  66
<212>  PRT
<213>  Saccharomyces eubayanus

<400>  79

Ala Pro Val Asn Thr Thr Thr Glu Asn Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Ser Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ala Asn Ser Thr Asn Thr Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ser Asn Leu Ala Ala Lys Glu Glu Gly Val Ser Leu Ser 
    50                  55                  60                  


Lys Arg 
65      


<210>  80
<211>  66
<212>  PRT
<213>  Saccharomyces kudriavzevii

<400>  80

Ala Pro Val Asn Thr Thr Ser Glu Ser Glu Thr Val Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Ile Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ala Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Asn Leu Ala Thr Lys Glu Glu Ser Val Pro Leu Ser 
    50                  55                  60                  


Lys Arg 
65      


