                         SEQUENCE LISTING

<110>  Cambridge Enterprise Limited
 
<120>  RNA Trans-Splicing Molecule

<130>  008176802

<140>  PCT/EP2022/
<141>  2022-02-11

<150>  GB2102118.3
<151>  2021-02-15

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HERV-K target sequence of binding region

<400>  1
tggataatcc tatagaagta tatgttaatg atagcgaatg ggta                        44


<210>  2
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HERV-K target sequence of binding region

<400>  2
uggauaaucc uauagaagua uauguuaaug auagcgaaug ggua                        44


<210>  3
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of HERV-K target sequence

<400>  3
tacccattcg ctatcattaa catatacttc tataggatta tcca                        44


<210>  4
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of HERV-K target sequence

<400>  4
uacccauucg cuaucauuaa cauauacuuc uauaggauua ucca                        44


<210>  5
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modified reverse complement of HERV-K target sequence

<400>  5
uacucauucg cuaucauuuu cauauacuuc ugugggauua ucca                        44


<210>  6
<211>  376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HSV Thymidine Kinase (HSV-tk) amino acid sequence

<400>  6

Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala 
1               5                   10                  15      


Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg 
            20                  25                  30          


Arg Gln Gln Glu Ala Thr Glu Val Arg Pro Glu Gln Lys Met Pro Thr 
        35                  40                  45              


Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr 
    50                  55                  60                  


Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr 
65                  70                  75                  80  


Val Pro Glu Pro Met Thr Tyr Trp Arg Val Leu Gly Ala Ser Glu Thr 
                85                  90                  95      


Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile 
            100                 105                 110         


Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met 
        115                 120                 125             


Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly 
    130                 135                 140                 


Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile 
145                 150                 155                 160 


Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg 
                165                 170                 175     


Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala 
            180                 185                 190         


Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu 
        195                 200                 205             


Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly 
    210                 215                 220                 


Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly 
225                 230                 235                 240 


Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg 
                245                 250                 255     


Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala 
            260                 265                 270         


Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu 
        275                 280                 285             


Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu 
    290                 295                 300                 


Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg 
305                 310                 315                 320 


Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys 
                325                 330                 335     


Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val 
            340                 345                 350         


Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe 
        355                 360                 365             


Ala Arg Glu Met Gly Glu Ala Asn 
    370                 375     


<210>  7
<211>  1131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HSV Thymidine Kinase (HSV-tk) coding sequence

<400>  7
atggcttcgt acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc       60

ggccatagca accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc      120

cgcccggagc agaaaatgcc cacgctactg cgggtttata tagacggtcc ccacgggatg      180

gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac      240

gtacccgagc cgatgactta ctggcgggtg ctgggggctt ccgagacaat cgcgaacatc      300

tacaccacac aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta      360

atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct      420

cctcatatcg ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc      480

ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc      540

agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc      600

acaaacatcg tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc      660

cagcgccccg gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg      720

ctgcttgcca atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga      780

cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca      840

cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc      900

aacggcgacc tgtataacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt      960

cccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg     1020

ctgcaactta cctccgggat ggtccagacc cacgtcacca ccccaggctc cataccgacg     1080

atctgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg a              1131


<210>  8
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified HSV Thymidine Kinase (HSV-tk) amino acid sequence

<400>  8

Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala Ala 
1               5                   10                  15      


Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg Arg 
            20                  25                  30          


Gln Gln Glu Ala Thr Glu Val Arg Pro Glu Gln Lys Leu Pro Thr Leu 
        35                  40                  45              


Leu Arg Val Tyr Ile Asp Gly Pro His Gly Ile Gly Lys Thr Thr Thr 
    50                  55                  60                  


Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr Val 
65                  70                  75                  80  


Pro Glu Pro Met Thr Tyr Trp Arg Val Leu Gly Ala Ser Glu Thr Ile 
                85                  90                  95      


Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile Ser 
            100                 105                 110         


Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met Gly 
        115                 120                 125             


Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly Gly 
    130                 135                 140                 


Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile Phe 
145                 150                 155                 160 


Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg Tyr 
                165                 170                 175     


Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala Leu 
            180                 185                 190         


Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu Pro 
        195                 200                 205             


Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly Glu 
    210                 215                 220                 


Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly Leu 
225                 230                 235                 240 


Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg Glu 
                245                 250                 255     


Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala Glu 
            260                 265                 270         


Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu Phe 
        275                 280                 285             


Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu Tyr 
    290                 295                 300                 


Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg Pro 
305                 310                 315                 320 


Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys Arg 
                325                 330                 335     


Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val Thr 
            340                 345                 350         


Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe Ala 
        355                 360                 365             


Arg Glu Met Gly Glu Ala Asn 
    370                 375 


<210>  9
<211>  1128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified HSV Thymidine Kinase (HSV-tk) coding sequence

<400>  9
gcttcgtacc cctgccatca acacgcgtct gcgttcgacc aggctgcgcg ttctcgcggc       60

catagcaacc gacgtacggc gttgcgccct cgccggcagc aagaagccac ggaagtccgc      120

ccggagcaga aactgcccac gctactgcgg gtttatatag acggtcccca cgggatcggg      180

aaaaccacca ccacgcaact gctggtggcc ctgggttcgc gcgacgatat cgtctacgta      240

cccgagccga tgacttactg gcgggtgctg ggggcttccg agacaatcgc gaacatctac      300

accacacaac accgcctcga ccagggtgag atatcggccg gggacgcggc ggtggtaatg      360

acaagcgccc agataacaat gggcatgcct tatgccgtga ccgacgccgt tctggctcct      420

catatcgggg gggaggctgg gagctcacat gccccgcccc cggccctcac cctcatcttc      480

gaccgccatc ccatcgccgc cctcctgtgc tacccggccg cgcgatacct tatgggcagc      540

atgacccccc aggccgtgct ggcgttcgtg gccctcatcc cgccgacctt gcccggcaca      600

aacatcgtgt tgggggccct tccggaggac agacacatcg accgcctggc caaacgccag      660

cgccccggcg agcggcttga cctggctatg ctggccgcga ttcgccgcgt ttacgggctg      720

cttgccaata cggtgcggta tctgcagggc ggcgggtcgt ggcgggagga ttggggacag      780

ctttcgggga cggccgtgcc gccccagggt gccgagcccc agagcaacgc gggcccacga      840

ccccatatcg gggacacgtt atttaccctg tttcgggccc ccgagttgct ggcccccaac      900

ggcgacctgt ataacgtgtt tgcctgggcc ttggacgtct tggccaaacg cctccgtccc      960

atgcacgtct ttatcctgga ttacgaccaa tcgcccgccg gctgccggga cgccctgctg     1020

caacttacct ccgggatggt ccagacccac gtcaccaccc caggctccat accgacgatc     1080

tgcgacctgg cgcgcacgtt tgcccgggag atgggggagg ctaactga                  1128


<210>  10
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer sequence

<400>  10
ggtgacgaaa acgtgctatc agttcgctcc cccactccc                              39


<210>  11
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A cleavage sequence

<400>  11

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trans-splicing domain sequence

<400>  12
gggcctgggc ctgggtacta acacgatcgt ttttttccct ttttttccag g                51


<210>  13
<211>  1511
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RTM sequence

<400>  13
gcuagcuacu cauucgcuau cauuuucaua uacuucugug ggauuaucca ccucgcggug       60

acgaaaacgu gcuaucaguu cgcuccccca cucccgcuuu cauuuuuguc uugucuuuuu      120

uuaaccuggg ccugggccug gguacuaaca cgaucguuuu uuucccuuuu uuuccagggg      180

aagcggagcu acuaacuuca gccugcugaa gcaggcugga gacguggagg agaacccugg      240

gccugcuucg uaccccugcc aucaacacgc gucugcguuc gaccaggcgg cgcgaucacg      300

gggacacagc aaccgacgga cggcguugcg cccucgccgg cagcaagaag ccacggaagu      360

ccgcccggag cagaaaaugc ccacgcuacu gcggguuuau auagacgguc cccacgggau      420

ggggaaaacc accaccacgc aacugcuggu ggcccugggu ucgcgcgacg auaucgucua      480

cguacccgag ccgaugacuu acuggcgggu gcugggggcu uccgagacaa ucgcgaacau      540

cuacaccaca caacaccgcc ucgaccaggu aaguaucaag guuacaagac agguuuaagg      600

agaccaauag aaacugggcu ugucgagaca gagacgacuc uugcguuucu gauaggcacc      660

uauuggucuu acugacaucc acuuugccuu ucucuccaca gggugagaua ucggccgggg      720

acgcggcggu gguaaugaca agcgcccaga uaacaauggg caugccuuau gccgugaccg      780

acgccguucu ggcuccucau aucggggggg aggcugggag cucacaugcc ccuccuccgg      840

cccucacccu caucuucgac cgccauccca ucgccgcccu ccugugcuac ccggccgcgc      900

gauaccuuau gggcagcaug accccccagg ccgugcuggc guucguggcc cucaucccgc      960

cgaccuugcc cggcacaaac aucguguugg gggcccuucc ggaggacaga cacaucgacc     1020

gccuggccaa acgccagcgc cccggcgagc ggcuugaccu ggcuaugcug gccgcgauuc     1080

gccgcguuua cgggcugcuu gccaauacgg ugcgguaucu gcagggcggc gggucguggc     1140

gggaggauug gggacagcuu ucggggacgg ccgugccgcc ucagggugcc gagccucaga     1200

gcaacgcggg cccacgaccc cauaucgggg acacguuauu uacccuguuu cgggcccccg     1260

aguugcuggc ccccaacggc gaccuguaua acguguuugc cugggccuug gacgucuugg     1320

ccaaacgccu ccgucccaug cacgucuuua uccuggauua cgaccaaucg cccgccggcu     1380

gccgggacgc ccugcugcaa cuuaccuccg ggauggucca gacccacguc accaccccag     1440

gcuccauacc gacgaucugc gaccuggcgc gcacguuugc gcgggagaug ggggaggcga     1500

acugacucga g                                                          1511


<210>  14
<211>  1511
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding DNA sequence for RTM

<400>  14
gctagctact cattcgctat cattttcata tacttctgtg ggattatcca cctcgcggtg       60

acgaaaacgt gctatcagtt cgctccccca ctcccgcttt catttttgtc ttgtcttttt      120

ttaacctggg cctgggcctg ggtactaaca cgatcgtttt tttccctttt tttccagggg      180

aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg      240

gcctgcttcg tacccctgcc atcaacacgc gtctgcgttc gaccaggcgg cgcgatcacg      300

gggacacagc aaccgacgga cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt      360

ccgcccggag cagaaaatgc ccacgctact gcgggtttat atagacggtc cccacgggat      420

ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta      480

cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat      540

ctacaccaca caacaccgcc tcgaccaggt aagtatcaag gttacaagac aggtttaagg      600

agaccaatag aaactgggct tgtcgagaca gagacgactc ttgcgtttct gataggcacc      660

tattggtctt actgacatcc actttgcctt tctctccaca gggtgagata tcggccgggg      720

acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat gccgtgaccg      780

acgccgttct ggctcctcat atcggggggg aggctgggag ctcacatgcc cctcctccgg      840

ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac ccggccgcgc      900

gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc ctcatcccgc      960

cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga cacatcgacc     1020

gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg gccgcgattc     1080

gccgcgttta cgggctgctt gccaatacgg tgcggtatct gcagggcggc gggtcgtggc     1140

gggaggattg gggacagctt tcggggacgg ccgtgccgcc tcagggtgcc gagcctcaga     1200

gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt cgggcccccg     1260

agttgctggc ccccaacggc gacctgtata acgtgtttgc ctgggccttg gacgtcttgg     1320

ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg cccgccggct     1380

gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc accaccccag     1440

gctccatacc gacgatctgc gacctggcgc gcacgtttgc gcgggagatg ggggaggcga     1500

actgactcga g                                                          1511


<210>  15
<211>  1511
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RTM sequence with modified HSV-tk coding sequence

<400>  15
gcuagcuacu cauucgcuau cauuuucaua uacuucugug ggauuaucca ccucgcggug       60

acgaaaacgu gcuaucaguu cgcuccccca cucccgcuuu cauuuuuguc uugucuuuuu      120

uuaaccuggg ccugggccug gguacuaaca cgaucguuuu uuucccuuuu uuuccagggg      180

aagcggagcu acuaacuuca gccugcugaa gcaggcugga gacguggagg agaacccugg      240

gccugcuucg uaccccugcc aucaacacgc gucugcguuc gaccaggcgg cgcgaucacg      300

gggacacagc aaccgacgga cggcguugcg cccucgccgg cagcaagaag ccacggaagu      360

ccgcccggag cagaaacugc ccacgcuacu gcggguuuau auagacgguc cccacgggau      420

cgggaaaacc accaccacgc aacugcuggu ggcccugggu ucgcgcgacg auaucgucua      480

cguacccgag ccgaugacuu acuggcgggu gcugggggcu uccgagacaa ucgcgaacau      540

cuacaccaca caacaccgcc ucgaccaggu aaguaucaag guuacaagac agguuuaagg      600

agaccaauag aaacugggcu ugucgagaca gagacgacuc uugcguuucu gauaggcacc      660

uauuggucuu acugacaucc acuuugccuu ucucuccaca gggugagaua ucggccgggg      720

acgcggcggu gguaaugaca agcgcccaga uaacaauggg caugccuuau gccgugaccg      780

acgccguucu ggcuccucau aucggggggg aggcugggag cucacaugcc ccuccuccgg      840

cccucacccu caucuucgac cgccauccca ucgccgcccu ccugugcuac ccggccgcgc      900

gauaccuuau gggcagcaug accccccagg ccgugcuggc guucguggcc cucaucccgc      960

cgaccuugcc cggcacaaac aucguguugg gggcccuucc ggaggacaga cacaucgacc     1020

gccuggccaa acgccagcgc cccggcgagc ggcuugaccu ggcuaugcug gccgcgauuc     1080

gccgcguuua cgggcugcuu gccaauacgg ugcgguaucu gcagggcggc gggucguggc     1140

gggaggauug gggacagcuu ucggggacgg ccgugccgcc ucagggugcc gagccucaga     1200

gcaacgcggg cccacgaccc cauaucgggg acacguuauu uacccuguuu cgggcccccg     1260

aguugcuggc ccccaacggc gaccuguaua acguguuugc cugggccuug gacgucuugg     1320

ccaaacgccu ccgucccaug cacgucuuua uccuggauua cgaccaaucg cccgccggcu     1380

gccgggacgc ccugcugcaa cuuaccuccg ggauggucca gacccacguc accaccccag     1440

gcuccauacc gacgaucugc gaccuggcgc gcacguuugc gcgggagaug ggggaggcga     1500

acugacucga g                                                          1511


<210>  16
<211>  1511
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding DNA sequence for RTM with modified HSV-tk coding sequence

<400>  16
gctagctact cattcgctat cattttcata tacttctgtg ggattatcca cctcgcggtg       60

acgaaaacgt gctatcagtt cgctccccca ctcccgcttt catttttgtc ttgtcttttt      120

ttaacctggg cctgggcctg ggtactaaca cgatcgtttt tttccctttt tttccagggg      180

aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg      240

gcctgcttcg tacccctgcc atcaacacgc gtctgcgttc gaccaggcgg cgcgatcacg      300

gggacacagc aaccgacgga cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt      360

ccgcccggag cagaaactgc ccacgctact gcgggtttat atagacggtc cccacgggat      420

cgggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta      480

cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat      540

ctacaccaca caacaccgcc tcgaccaggt aagtatcaag gttacaagac aggtttaagg      600

agaccaatag aaactgggct tgtcgagaca gagacgactc ttgcgtttct gataggcacc      660

tattggtctt actgacatcc actttgcctt tctctccaca gggtgagata tcggccgggg      720

acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat gccgtgaccg      780

acgccgttct ggctcctcat atcggggggg aggctgggag ctcacatgcc cctcctccgg      840

ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac ccggccgcgc      900

gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc ctcatcccgc      960

cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga cacatcgacc     1020

gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg gccgcgattc     1080

gccgcgttta cgggctgctt gccaatacgg tgcggtatct gcagggcggc gggtcgtggc     1140

gggaggattg gggacagctt tcggggacgg ccgtgccgcc tcagggtgcc gagcctcaga     1200

gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt cgggcccccg     1260

agttgctggc ccccaacggc gacctgtata acgtgtttgc ctgggccttg gacgtcttgg     1320

ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg cccgccggct     1380

gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc accaccccag     1440

gctccatacc gacgatctgc gacctggcgc gcacgtttgc gcgggagatg ggggaggcga     1500

actgactcga g                                                          1511


<210>  17
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding P2A peptide

<400>  17
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct       60

gggcct                                                                  66


<210>  18
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BbvCI restriction enzyme site

<400>  18
cctcagcagt g                                                            11


<210>  19
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BbvCI restriction enzyme site

<400>  19
cctcgcggtg                                                              10


<210>  20
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Polypyrimidine tract

<400>  20
uuuuuuuccc uuuuuuucc                                                    19


<210>  21
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intronic splice enhancer

<400>  21
gggcctgggc ctggg                                                        15


<210>  22
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor site

<400>  22
cagguaagta t                                                            11


<210>  23
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site-directed mutagenesis forward primer

<400>  23
gaaactgccc acgctactgc gggtttatat agacggtccc cacgggatcg gg               52


<210>  24
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site-directed mutagenesis reverse primer

<400>  24
cccgatcccg tggggaccgt ctatataaac ccgcagtagc gtgggcagtt tc               52


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis forward primer tktr 3 mut

<400>  25
acggtcccca cgggatcggg aaaaccacca c                                      31


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis reverse primer tktr 3 mut

<400>  26
gtggtggttt tcccgatccc gtggggaccg t                                      31


<210>  27
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XbaI forward  primer

<400>  27
gacattgatt attgtctaga actagttgag ccccacg                                37


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XbaI reverse primer

<400>  28
cgtggggctc aactagttct agacaataat caatgtc                                37


<210>  29
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XhoI forward primer

<400>  29
gggaggcgaa ctgactcgag aacttgttta ttgc                                   34


<210>  30
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XhoI reverse primer

<400>  30
gcaataaaca agttctcgag tcagttcgcc tccc                                   34


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TG BcuI forward primer

<400>  31
gcactagttg catgtataat ttctacag                                          28


<210>  32
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TG NheI reverse primer

<400>  32
cggctagctt atagcatgtc ctgtattg                                          28


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Psi forward primer

<400>  33
caggactcgg cttgctgaag                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Psi reverse primer

<400>  34
tcccccgctt aatactgacg                                                   20


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Albumin forward primer

<400>  35
gctgtcatct cttgtgggct gt                                                22


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Albumin reverse primer

<400>  36
actcatggga gctgctggtt c                                                 21


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE forward primer

<400>  37
ggcactgaca attccgtggt                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE reverse primer

<400>  38
agggacgtag cagaaggacg                                                   20


<210>  39
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE probe

<400>  39
acgtcctttc catggctgct cgc                                               23


<210>  40
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding domain sequence of targeting Np9

<400>  40
tactcattcg ctatcatttt catatacttc tgtgggatta tcca                        44


