                         SEQUENCE LISTING

<110>  Intervet International BV
 
<120>  Vaccine for Mycoplasma bovis

<130>  25111

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Gly Xaa Ser Gly 
1               


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide

<400>  2

Gly Gly Ser Gly 
1               


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide

<400>  3

Gly Ala Ser Gly 
1               


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide

<400>  4

Gly Ser Ser Gly 
1               


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide

<400>  5

Gly Thr Ser Gly 
1               


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide

<400>  6

Gly Asn Ser Gly 
1               


<210>  7
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  7

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  8
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  8

Asp Lys Lys Glu Glu Lys Lys Lys Val Glu Glu Pro Ala Lys Gln Ala 
1               5                   10                  15      


Glu Gly 
        


<210>  9
<211>  20
<212>  PRT
<213>  Mycoplasma bovis

<400>  9

Glu Lys Glu Ile Lys Glu Thr Ser Asp Ser Pro Lys Lys Asp Gly Glu 
1               5                   10                  15      


Lys Val Ser Asp 
            20  


<210>  10
<211>  16
<212>  PRT
<213>  Mycoplasma bovis

<400>  10

Pro Val Thr Thr Glu Asn Asn Asp Ser Ile Lys Asp Val Lys Ser Asp 
1               5                   10                  15      


<210>  11
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  11

Asp Asn Thr Tyr Leu Glu Gln Val Lys Leu Asp Ser Val Ser Asp Val 
1               5                   10                  15      


Ile Lys 
        


<210>  12
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  12

Ala Lys Lys Asp Tyr Gly Phe Asn Ser His Phe Gly Glu Asp Phe 
1               5                   10                  15  


<210>  13
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  13

Val Ser Asp Phe Ile Ala Gly Ala Phe Gln Gly Ile Ile Asp Trp 
1               5                   10                  15  


<210>  14
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  14

Val Ser Asp Phe Ile Ala Gly Thr Phe Gln Gly Ile Ile Asp Trp 
1               5                   10                  15  


<210>  15
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  15

Arg Glu Leu Ala Leu Asp Glu Tyr Ser Leu Arg Ile Glu Tyr Ser Ala 
1               5                   10                  15      


Ser Glu 
        


<210>  16
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  16

Ile Val Thr Asn Asp Phe Ile Leu Ile Gly Gly Asp Gly Tyr Lys 
1               5                   10                  15  


<210>  17
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  17

Lys Ala Lys Ser Val Phe Glu Gly Gly Asp Ile Leu Glu Ser Tyr 
1               5                   10                  15  


<210>  18
<211>  21
<212>  PRT
<213>  Mycoplasma bovis

<400>  18

Asn Glu Thr Lys Glu Pro Lys Lys Glu Pro Glu Met Asp Ala Pro Ile 
1               5                   10                  15      


Ala Pro Pro Thr Asp 
            20      


<210>  19
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  19

Gly Glu Gly Gln Asn Asn Gly Lys Pro Gly Glu Asp Lys Pro Asn 
1               5                   10                  15  


<210>  20
<211>  21
<212>  PRT
<213>  Mycoplasma bovis

<400>  20

Gly Thr Asn Thr Asn Asn Asn Asn Thr Pro Met Pro Asn Glu Asn Ala 
1               5                   10                  15      


Thr Pro Ser Pro Asn 
            20      


<210>  21
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  21

Asp Val Glu Glu Phe Met Asp Phe Ala Val Ile Glu Ile Asp Phe 
1               5                   10                  15  


<210>  22
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  22

Tyr Ser Lys Ile Asp Arg Pro Ile Ile Leu Asp Thr Lys Lys Lys Glu 
1               5                   10                  15      


Glu Val 
        


<210>  23
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  23

Gly Asp Tyr Phe Leu Glu Asn Tyr Ile Asp Glu Asp Gln Gln Lys 
1               5                   10                  15  


<210>  24
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  24

Lys Ala Lys Asn Asp Gln Pro Leu Glu Asp Phe Asp Lys Glu Ile 
1               5                   10                  15  


<210>  25
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  25

Asp Lys Phe Asp Val Asn Val Ser Ser Phe Ile Asp Asp Ser Phe 
1               5                   10                  15  


<210>  26
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  26

Asn Thr Glu Pro Gly Lys Asn Pro Gly Gly Asn Thr Glu Pro Gly Lys 
1               5                   10                  15      


Asn Pro 
        


<210>  27
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  27

Pro Glu Thr Pro Lys Gly Pro Glu Thr Pro Lys Gly Pro Glu Thr Pro 
1               5                   10                  15      


Lys Gly 
        


<210>  28
<211>  18
<212>  PRT
<213>  Mycoplasma bovis

<400>  28

Pro Glu Ala Pro Lys Gly Pro Glu Ala Pro Lys Gly Pro Glu Ala Pro 
1               5                   10                  15      


Lys Gly 
        


<210>  29
<211>  16
<212>  PRT
<213>  Mycoplasma bovis

<400>  29

Lys Asn Pro Gly Gly Asp Lys Asn Pro Gly Gly Asp Lys Asn Pro Gly 
1               5                   10                  15      


<210>  30
<211>  15
<212>  PRT
<213>  Mycoplasma bovis

<400>  30

Gly Glu Asn Thr Glu Pro Asp Lys Asn Pro Gly Gly Asp Lys Asn 
1               5                   10                  15  


<210>  31
<211>  209
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope construct 1a

<400>  31

Gly Gly Ser Gly Asp Lys Lys Glu Glu Lys Lys Lys Val Glu Glu Pro 
1               5                   10                  15      


Ala Lys Gln Ala Glu Gly Gly Ala Ser Gly Glu Lys Glu Ile Lys Glu 
            20                  25                  30          


Thr Ser Asp Ser Pro Lys Lys Asp Gly Glu Lys Val Ser Asp Gly Ser 
        35                  40                  45              


Ser Gly Pro Val Thr Thr Glu Asn Asn Asp Ser Ile Lys Asp Val Lys 
    50                  55                  60                  


Ser Asp Gly Thr Ser Gly Asp Asn Thr Tyr Leu Glu Gln Val Lys Leu 
65                  70                  75                  80  


Asp Ser Val Ser Asp Val Ile Lys Gly Asn Ser Gly Ala Lys Lys Asp 
                85                  90                  95      


Tyr Gly Phe Asn Ser His Phe Gly Glu Asp Phe Gly Gly Ser Gly Val 
            100                 105                 110         


Ser Asp Phe Ile Ala Gly Thr Phe Gln Gly Ile Ile Asp Trp Gly Ala 
        115                 120                 125             


Ser Gly Val Ser Asp Phe Ile Ala Gly Ala Phe Gln Gly Ile Ile Asp 
    130                 135                 140                 


Trp Gly Ser Ser Gly Arg Glu Leu Ala Leu Asp Glu Tyr Ser Leu Arg 
145                 150                 155                 160 


Ile Glu Tyr Ser Ala Ser Glu Gly Thr Ser Gly Ile Val Thr Asn Asp 
                165                 170                 175     


Phe Ile Leu Ile Gly Gly Asp Gly Tyr Lys Gly Asn Ser Gly Lys Ala 
            180                 185                 190         


Lys Ser Val Phe Glu Gly Gly Asp Ile Leu Glu Ser Tyr Gly Gly Ser 
        195                 200                 205             


Gly 
    


<210>  32
<211>  209
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope construct 1b

<400>  32

Gly Gly Ser Gly Ala Lys Lys Asp Tyr Gly Phe Asn Ser His Phe Gly 
1               5                   10                  15      


Glu Asp Phe Gly Ala Ser Gly Val Ser Asp Phe Ile Ala Gly Thr Phe 
            20                  25                  30          


Gln Gly Ile Ile Asp Trp Gly Ser Ser Gly Val Ser Asp Phe Ile Ala 
        35                  40                  45              


Gly Ala Phe Gln Gly Ile Ile Asp Trp Gly Thr Ser Gly Ile Val Thr 
    50                  55                  60                  


Asn Asp Phe Ile Leu Ile Gly Gly Asp Gly Tyr Lys Gly Asn Ser Gly 
65                  70                  75                  80  


Lys Ala Lys Ser Val Phe Glu Gly Gly Asp Ile Leu Glu Ser Tyr Gly 
                85                  90                  95      


Gly Ser Gly Arg Glu Leu Ala Leu Asp Glu Tyr Ser Leu Arg Ile Glu 
            100                 105                 110         


Tyr Ser Ala Ser Glu Gly Ala Ser Gly Asp Lys Lys Glu Glu Lys Lys 
        115                 120                 125             


Lys Val Glu Glu Pro Ala Lys Gln Ala Glu Gly Gly Ser Ser Gly Glu 
    130                 135                 140                 


Lys Glu Ile Lys Glu Thr Ser Asp Ser Pro Lys Lys Asp Gly Glu Lys 
145                 150                 155                 160 


Val Ser Asp Gly Thr Ser Gly Asp Asn Thr Tyr Leu Glu Gln Val Lys 
                165                 170                 175     


Leu Asp Ser Val Ser Asp Val Ile Lys Gly Asn Ser Gly Pro Val Thr 
            180                 185                 190         


Thr Glu Asn Asn Asp Ser Ile Lys Asp Val Lys Ser Asp Gly Gly Ser 
        195                 200                 205             


Gly 
    


<210>  33
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope construct 2a

<400>  33

Gly Gly Ser Gly Asn Glu Thr Lys Glu Pro Lys Lys Glu Pro Glu Met 
1               5                   10                  15      


Asp Ala Pro Ile Ala Pro Pro Thr Asp Gly Ala Ser Gly Gly Thr Asn 
            20                  25                  30          


Thr Asn Asn Asn Asn Thr Pro Met Pro Asn Glu Asn Ala Thr Pro Ser 
        35                  40                  45              


Pro Asn Gly Ser Ser Gly Gly Glu Gly Gln Asn Asn Gly Lys Pro Gly 
    50                  55                  60                  


Glu Asp Lys Pro Asn Gly Thr Ser Gly Asp Val Glu Glu Phe Met Asp 
65                  70                  75                  80  


Phe Ala Val Ile Glu Ile Asp Phe Gly Asn Ser Gly Tyr Ser Lys Ile 
                85                  90                  95      


Asp Arg Pro Ile Ile Leu Asp Thr Lys Lys Lys Glu Glu Val Gly Gly 
            100                 105                 110         


Ser Gly Gly Asp Tyr Phe Leu Glu Asn Tyr Ile Asp Glu Asp Gln Gln 
        115                 120                 125             


Lys Gly Ala Ser Gly Lys Ala Lys Asn Asp Gln Pro Leu Glu Asp Phe 
    130                 135                 140                 


Asp Lys Glu Ile Gly Ser Ser Gly Asp Lys Phe Asp Val Asn Val Ser 
145                 150                 155                 160 


Ser Phe Ile Asp Asp Ser Phe Gly Gly Ser Gly 
                165                 170     


<210>  34
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope construct 2b

<400>  34

Gly Gly Ser Gly Lys Ala Lys Asn Asp Gln Pro Leu Glu Asp Phe Asp 
1               5                   10                  15      


Lys Glu Ile Gly Ala Ser Gly Asp Lys Phe Asp Val Asn Val Ser Ser 
            20                  25                  30          


Phe Ile Asp Asp Ser Phe Gly Ser Ser Gly Asp Val Glu Glu Phe Met 
        35                  40                  45              


Asp Phe Ala Val Ile Glu Ile Asp Phe Gly Thr Ser Gly Tyr Ser Lys 
    50                  55                  60                  


Ile Asp Arg Pro Ile Ile Leu Asp Thr Lys Lys Lys Glu Glu Val Gly 
65                  70                  75                  80  


Asn Ser Gly Gly Asp Tyr Phe Leu Glu Asn Tyr Ile Asp Glu Asp Gln 
                85                  90                  95      


Gln Lys Gly Gly Ser Gly Gly Glu Gly Gln Asn Asn Gly Lys Pro Gly 
            100                 105                 110         


Glu Asp Lys Pro Asn Gly Ala Ser Gly Gly Thr Asn Thr Asn Asn Asn 
        115                 120                 125             


Asn Thr Pro Met Pro Asn Glu Asn Ala Thr Pro Ser Pro Asn Gly Ser 
    130                 135                 140                 


Ser Gly Asn Glu Thr Lys Glu Pro Lys Lys Glu Pro Glu Met Asp Ala 
145                 150                 155                 160 


Pro Ile Ala Pro Pro Thr Asp Gly Gly Ser Gly 
                165                 170     


<210>  35
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope construct 3

<400>  35

Gly Gly Ser Gly Lys Asn Pro Gly Gly Asp Lys Asn Pro Gly Gly Asp 
1               5                   10                  15      


Lys Asn Pro Gly Gly Ala Ser Gly Gly Glu Asn Thr Glu Pro Asp Lys 
            20                  25                  30          


Asn Pro Gly Gly Asp Lys Asn Gly Ser Ser Gly Pro Glu Thr Pro Lys 
        35                  40                  45              


Gly Pro Glu Thr Pro Lys Gly Pro Glu Thr Pro Lys Gly Gly Thr Ser 
    50                  55                  60                  


Gly Pro Glu Ala Pro Lys Gly Pro Glu Ala Pro Lys Gly Pro Glu Ala 
65                  70                  75                  80  


Pro Lys Gly Gly Asn Ser Gly Asn Thr Glu Pro Gly Lys Asn Pro Gly 
                85                  90                  95      


Gly Asn Thr Glu Pro Gly Lys Asn Pro Gly Gly Ser Gly 
            100                 105                 


<210>  36
<211>  627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid for epitope construct 1a

<400>  36
ggtggtagtg gtgataaaaa agaagaaaaa aaaaaagtcg aagaacccgc caaacaagcc       60

gaaggtggtg cctcaggtga aaaagaaatt aaagaaacct cagattcacc caaaaaagat      120

ggtgaaaaag tctcagatgg ttcatcaggt cccgtcacca ccgaaaataa tgattcaatt      180

aaagatgtca aatcagatgg tacctcaggt gataatacct acttagaaca agtcaaatta      240

gattcagtct cagatgtcat taaaggtaat tcaggtgcca aaaaagatta cggtttcaat      300

tcacacttcg gtgaagattt cggtggttca ggtgtctcag atttcattgc cggtacattc      360

caaggtatca ttgattgagg tgcctcaggc gtctccgatt tcattgccgg tgccttccaa      420

ggtattattg attgaggttc tagcggtaga gaattagcct tagatgaata ctcattaaga      480

attgaatact cagcctcaga aggtacatca ggtattgtca ccaatgactt cattttaatt      540

ggtggcgatg gttacaaagg caactcaggt aaagccaaat cagtcttcga aggtggtgat      600

attttagaat catacggtgg ttcgggt                                          627


<210>  37
<211>  627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid for epitope construct 1b

<400>  37
ggtggtagtg gtgccaaaaa agattacggt ttcaattcac acttcggtga agatttcggt       60

gcctcaggtg tctcagattt cattgccggt accttccaag gtattattga ttgaggttca      120

tcaggtgtct cagatttcat tgccggtgcc ttccaaggta ttattgattg aggtacctca      180

ggtatcgtca caaatgattt cattttaatt ggtggtgacg gttacaaagg caattcaggt      240

aaagccaaat cagtcttcga aggtggtgat attttagaat catacggtgg ttcaggtaga      300

gaattagcct tagatgaata ctcattaaga attgaatact cagcctcaga aggtgcctca      360

ggtgataaaa aagaagaaaa aaaaaaagtc gaagaacccg ccaaacaagc cgaaggtggt      420

tcatcaggtg aaaaagaaat taaagaaacc tcagattcac ccaaaaaaga tggtgaaaaa      480

gtctcagatg gtacctcagg tgataatacc tacttagaac aagtcaaatt agattcagtc      540

tcagatgtca ttaaaggtaa ctcaggtccc gtcaccaccg aaaataatga ttcaattaaa      600

gatgtcaaat cagatggtgg ttcaggt                                          627


<210>  38
<211>  513
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid for epitope construct 2a

<400>  38
ggtggtagtg gcaatgaaac caaagaaccc aaaaaagaac ccgaaatgga tgcccccatt       60

gcccccccca ccgatggtgc ctcaggtggt accaatacca ataataataa tacccccatg      120

cccaatgaaa atgccacccc ctcacccaat ggttcatcag gtggtgaagg tcaaaataat      180

ggtaaacccg gtgaagataa acccaatggt acctcaggtg atgtcgaaga attcatggat      240

ttcgccgtca ttgaaattga tttcggtaat tcaggttact caaaaattga tagacccatt      300

attttagata ccaaaaaaaa agaagaagtc ggtggttcag gtggtgatta cttcttagaa      360

aattacattg atgaagatca acaaaaaggt gccagcggta aagccaaaaa tgatcaaccc      420

ttagaagatt tcgataaaga aattggttct agcggtgata aattcgatgt caatgtctca      480

tcattcattg atgattcatt cggtggttcg ggt                                   513


<210>  39
<211>  513
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid for epitope construct 2b

<400>  39
ggtggtagtg gtaaagccaa aaatgatcaa cccttagaag atttcgataa agaaattggt       60

gcctcaggtg ataaattcga cgtcaatgtc tcctccttca ttgacgattc attcggttca      120

tcaggtgatg tcgaagaatt catggacttc gccgtcattg aaattgattt cggtacctca      180

ggttactcaa aaattgatag acccattatt ttagatacca aaaaaaaaga agaagtcggt      240

aattcaggtg gtgattactt cttagaaaat tacattgatg aagatcaaca aaaaggtggt      300

tcaggtggtg aaggtcaaaa taatggtaaa cccggtgaag ataaacccaa tggtgcctct      360

ggtggtacca ataccaataa taataatacc cccatgccca atgaaaatgc caccccctca      420

cccaatggtt ctagcggtaa tgaaaccaaa gaacccaaaa aagaacccga aatggatgcc      480

cccattgccc cccccaccga tggtggttcg ggt                                   513


<210>  40
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid for epitope construct 3

<400>  40
ggtggtagtg gtaaaaatcc cggtggtgat aaaaatcccg gtggtgataa aaatcccggt       60

ggtgcctcag gtggtgaaaa taccgaaccc gataaaaatc ccggtggtga taaaaatggt      120

tcatcaggtc ccgaaacccc caaaggtccc gaaaccccca aaggtcccga aacccccaaa      180

ggtggtacct caggtcccga agcccccaaa ggtcccgaag cccccaaagg tcccgaagcc      240

cccaaaggtg gtaattcagg taataccgaa cccggtaaaa atcccggcgg taatacagag      300

cccggtaaaa atcccggtgg ttcgggt                                          327


<210>  41
<211>  5
<212>  PRT
<213>  Mycoplasma bovis

<400>  41

Thr Pro Gly Glu Asn 
1               5   


<210>  42
<211>  4
<212>  PRT
<213>  Mycoplasma bovis

<400>  42

Ser Lys Lys Ser 
1               


