                         SEQUENCE LISTING

<110>  Technische Universitt Graz
 
<120>  ENANTIOSELECTIVE HYDROLYSIS

<130>  29636-WO

<150>  EP21156519.7
<151>  11.02.2021

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  392
<212>  PRT
<213>  Burkholderia gladioli

<400>  1

Met Thr Ala Ala Ser Leu Asp Pro Thr Ala Phe Ser Leu Asp Ala Ala 
1               5                   10                  15      


Ser Leu Ala Ala Arg Leu Asp Ala Val Phe Asp Gln Ala Leu Arg Glu 
            20                  25                  30          


Arg Arg Leu Val Gly Ala Val Ala Ile Val Ala Arg His Gly Glu Ile 
        35                  40                  45              


Leu Tyr Arg Arg Ala Gln Gly Leu Ala Asp Arg Glu Ala Gly Arg Pro 
    50                  55                  60                  


Met Arg Glu Asp Thr Leu Phe Arg Leu Ala Ser Val Thr Lys Pro Ile 
65                  70                  75                  80  


Val Ala Leu Ala Val Leu Arg Leu Val Ala Arg Gly Glu Leu Ala Leu 
                85                  90                  95      


Asp Ala Pro Val Thr Arg Trp Leu Pro Glu Phe Arg Pro Arg Leu Ala 
            100                 105                 110         


Asp Gly Ser Glu Pro Leu Val Thr Ile His His Leu Leu Thr His Thr 
        115                 120                 125             


Ser Gly Leu Gly Tyr Trp Leu Leu Glu Gly Ala Gly Ser Val Tyr Asp 
    130                 135                 140                 


Arg Leu Gly Ile Ser Asp Gly Ile Asp Leu Arg Asp Phe Asp Leu Asp 
145                 150                 155                 160 


Glu Asn Leu Arg Arg Leu Ala Ser Ala Pro Leu Ser Phe Ala Pro Gly 
                165                 170                 175     


Ser Gly Trp Gln Tyr Ser Leu Ala Leu Asp Val Leu Gly Ala Val Val 
            180                 185                 190         


Glu Arg Ala Thr Gly Gln Pro Leu Ala Ala Ala Val Asp Ala Leu Val 
        195                 200                 205             


Ala Gln Pro Leu Gly Met Arg Asp Cys Gly Phe Val Ser Ala Glu Pro 
    210                 215                 220                 


Glu Arg Phe Ala Val Pro Tyr His Asp Gly Gln Pro Glu Pro Val Arg 
225                 230                 235                 240 


Met Arg Asp Gly Ile Glu Val Pro Leu Pro Glu Gly His Gly Ala Ala 
                245                 250                 255     


Val Arg Phe Ala Pro Ser Arg Val Phe Glu Pro Gly Ala Tyr Pro Ser 
            260                 265                 270         


Gly Gly Ala Gly Met Tyr Gly Ser Ala Asp Asp Val Leu Arg Ala Leu 
        275                 280                 285             


Glu Ala Ile Arg Ala Asn Pro Gly Phe Leu Pro Glu Thr Leu Ala Asp 
    290                 295                 300                 


Ala Ala Arg Arg Asp Gln Ala Gly Val Gly Ala Glu Thr Arg Gly Pro 
305                 310                 315                 320 


Gly Trp Gly Phe Gly Tyr Leu Ser Ala Val Leu Asp Asp Pro Ala Ala 
                325                 330                 335     


Ala Gly Thr Pro Gln His Ala Gly Thr Leu Gln Trp Gly Gly Val Tyr 
            340                 345                 350         


Gly His Ser Trp Phe Val Asp Arg Ala Leu Gly Leu Ser Val Leu Leu 
        355                 360                 365             


Leu Thr Asn Thr Ala Tyr Glu Gly Met Ser Gly Pro Leu Thr Ile Ala 
    370                 375                 380                 


Leu Arg Asp Ala Val Tyr Ala Arg 
385                 390         


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Catalytic motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  X is any amino acid

<400>  2

Ser Xaa Xaa Lys 
1               


<210>  3
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NK70 mutant of esterase B of B. gladioli

<400>  3

Met Thr Ala Ala Ser Leu Asp Pro Thr Ala Phe Ser Leu Asp Ala Ala 
1               5                   10                  15      


Leu Leu Ala Ala Arg Leu Asp Ala Val Phe Asp Gln Ala Leu Arg Glu 
            20                  25                  30          


Arg Arg Leu Val Gly Ala Val Ala Ile Val Ala Arg His Gly Glu Ile 
        35                  40                  45              


Leu Tyr Arg Arg Ala Gln Gly Leu Ala Asp Arg Glu Ala Gly Arg Pro 
    50                  55                  60                  


Met Arg Glu Asp Thr Leu Phe Arg Leu Ala Ser Val Thr Lys Pro Ile 
65                  70                  75                  80  


Val Ala Leu Ala Val Leu Arg Leu Val Ala Arg Gly Glu Leu Ala Leu 
                85                  90                  95      


Asp Ala Pro Val Thr Arg Trp Leu Pro Glu Phe Arg Pro Arg Leu Ala 
            100                 105                 110         


Asp Gly Ser Glu Pro Leu Val Thr Ile His His Leu Leu Thr His Thr 
        115                 120                 125             


Ser Gly Leu Ser Tyr Trp Leu Leu Glu Gly Ala Gly Ser Val Tyr Asp 
    130                 135                 140                 


Arg Leu Gly Ile Ser Asp Gly Ile Asp Leu Arg Asp Phe Asp Leu Asp 
145                 150                 155                 160 


Glu Asn Leu Arg Arg Leu Ala Ser Ala Pro Leu Ser Phe Ala Pro Gly 
                165                 170                 175     


Ser Gly Trp Gln Tyr Ser Leu Ala Leu Asp Val Leu Gly Ala Val Val 
            180                 185                 190         


Glu Arg Ala Thr Gly Gln Pro Leu Ala Ala Ala Val Asp Ala Leu Val 
        195                 200                 205             


Ala Gln Pro Leu Gly Met Arg Asp Cys Gly Phe Val Ser Ala Glu Pro 
    210                 215                 220                 


Glu Arg Phe Ala Val Pro Tyr His Asp Gly Gln Pro Glu Pro Val Arg 
225                 230                 235                 240 


Met Arg Asp Gly Ile Glu Val Pro Leu Pro Gly Gly His Gly Ala Ala 
                245                 250                 255     


Val Arg Phe Ala Pro Ser Arg Val Phe Glu Pro Gly Ala Tyr Pro Ser 
            260                 265                 270         


Gly Gly Ala Gly Met Tyr Gly Ser Ala Asp Asp Val Leu Arg Ala Leu 
        275                 280                 285             


Glu Ala Ile Arg Ala Asn Pro Gly Phe Leu Pro Glu Thr Leu Ala Asp 
    290                 295                 300                 


Ala Ala Arg Arg Asp Gln Val Gly Val Gly Ala Lys Thr Arg Gly Pro 
305                 310                 315                 320 


Gly Trp Gly Phe Gly Tyr Leu Ser Ala Val Leu Asp Asp Pro Ala Ala 
                325                 330                 335     


Ala Gly Thr Pro Gln His Ala Gly Thr Leu Gln Trp Gly Gly Val Tyr 
            340                 345                 350         


Gly His Ser Trp Phe Val Asp Arg Ala Leu Gly Leu Ser Val Leu Leu 
        355                 360                 365             


Leu Thr Asn Thr Ala Tyr Glu Gly Met Ser Gly Pro Leu Thr Ile Ala 
    370                 375                 380                 


Leu Arg Asp Ala Val Tyr Ala Arg 
385                 390         


<210>  4
<211>  493
<212>  PRT
<213>  Rhodococcus rhodochrous

<400>  4

Met Glu Pro Val Val Asp Thr Thr Gly Gly Arg Val Arg Gly Thr Thr 
1               5                   10                  15      


Val Gly Gly Thr Ala Val Phe Lys Gly Ile Pro Tyr Ala Ala Ala Pro 
            20                  25                  30          


Phe Gly Glu Leu Arg Phe Ala Ala Pro Gly Pro Val Pro Arg Trp Asp 
        35                  40                  45              


Gly Val Arg Pro Ala Thr Glu Phe Gly Pro Thr Ala Pro Lys Ala Pro 
    50                  55                  60                  


Tyr Ala Pro Pro Ile Asp Gln Leu Leu His Glu Pro Ser Ile Pro Gly 
65                  70                  75                  80  


Glu Asp Val Leu Gly Leu Asn Val Trp Ser Pro Asp Leu Ser Gly Ser 
                85                  90                  95      


Leu Pro Val Ile Val Trp Ile His Gly Gly Ala Phe Val His Gly Ser 
            100                 105                 110         


Ser Ala Val Ala Leu Tyr Asp Gly Ala Asn Phe Ala Arg Asp Gly Val 
        115                 120                 125             


Val Phe Leu Ser Ile Asn Tyr Arg Leu Gly Ala Glu Gly Phe Ala Phe 
    130                 135                 140                 


Leu Asp Asp Ala Pro Ala Asn Arg Gly Leu Leu Asp Gln Val Ala Ala 
145                 150                 155                 160 


Leu Ala Trp Val Arg Asp Asn Ile Ala Ala Phe Gly Gly Asp Pro Asp 
                165                 170                 175     


Arg Val Thr Ile Ala Gly Glu Ser Ala Gly Ala Met Ser Val Val Ser 
            180                 185                 190         


Leu Leu Ala Met Pro Ala Ala Arg Gly Leu Phe Arg Arg Ala Ile Ala 
        195                 200                 205             


Gln Ser Gly Ala Gly His Ser Val Met Ser Ala Gly Thr Ala Arg Thr 
    210                 215                 220                 


Val Ala Gly Ala Leu Gly Asp Met Leu Gly Val Ala Pro Thr Arg Ala 
225                 230                 235                 240 


Ala Phe Ala Glu Ile Asp Pro Lys Val Leu Val Asp Ala Gln Glu Arg 
                245                 250                 255     


Leu Thr Ala Arg Ile Arg Gly Glu Arg Asp Pro Ala Thr Trp Gly Glu 
            260                 265                 270         


Ile Ala Thr Asp Ser Met Ala Phe Glu Pro Cys Val Asp Gly Thr Val 
        275                 280                 285             


Leu Pro Asp Arg Pro Ile Asp Leu Val Ala Asn Gly Ala Gly Ser Asp 
    290                 295                 300                 


Val Asp Val Leu Ile Gly His Asn Gly Asp Glu Met Thr Leu Phe Phe 
305                 310                 315                 320 


Val Pro Phe Gly Val Asp Gly Leu Leu Asp Glu Val Ala Ala Arg Gly 
                325                 330                 335     


Leu Val Ala Ala Tyr Gly Leu Thr Asp Pro Glu Ala Phe Asp Ala Tyr 
            340                 345                 350         


Arg Arg Ala Arg Pro Asp Ala Arg Pro Gly Asp Thr Ala Met His Ile 
        355                 360                 365             


Val Arg Asp Trp Met Tyr Arg Ile Pro Val Leu Arg Val Ala Glu Ala 
    370                 375                 380                 


Arg Ala Thr His Gly Ala Glu Thr His Val Tyr Arg Phe Asp Trp Lys 
385                 390                 395                 400 


Ser Pro Leu Phe Asp Gly Arg Leu Gly Ala Thr His Ala Leu Asp Ile 
                405                 410                 415     


Gly Phe Val Phe Asp Asn Leu His Leu Pro Gln Ala Ala Ala Leu Ala 
            420                 425                 430         


Gly Ser Ala Pro Pro Gln Glu Leu Ala Thr Arg Met His Arg Ala Trp 
        435                 440                 445             


Val Asp Phe Ala Thr Thr Gly Ser Pro Gly Trp Pro Pro Tyr Gly Asp 
    450                 455                 460                 


Glu Arg Thr Glu Met Leu Phe Gly Asp Thr Cys Gly Thr Val Asp Asp 
465                 470                 475                 480 


Ala Glu Ala Glu Leu Arg Thr Leu Trp Asp Gly Ile Arg 
                485                 490             


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Seqence

<220>
<223>  Catalytic motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  X is a chain of amino acids of a length of 20 to 200 amino acids

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X is a chain of amino acids of a length of 20 to 200 amino acids

<400>  5

Ser Xaa Glu Xaa His 
1               5   


