                         SEQUENCE LISTING

<110>  Procomcure Biotech GmbH
 
<120>  Assays for the Detection of SARS-CoV-2 Mutants

<130>  211182EP

<150>  21156411.7
<151>  2021-02-10

<150>  21156929.8
<151>  2021-02-12

<150>  21157716.8
<151>  2021-02-17

<150>  21158203.6
<151>  2021-02-19

<150>  21172801.9
<151>  2021-05-07

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  63
<212>  DNA
<213>  Homo sapiens

<400>  1
actttccttt acaatcatat ggtttccaac ccactaatgg tgttggttac caaccataca       60

gag                                                                     63


<210>  2
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence (A) mutant N501Y

<400>  2
actttccttt acaatcatat ggtttccaac ccacttatgg tgttggttac caaccataca       60

gag                                                                     63


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (A)

<400>  3
actttccttt acaatcatat gg                                                22


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (A)

<400>  4
ctctgtatgg ttggtaacc                                                    19


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (A)

<400>  5
tccaacccac taatggtg                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (A)

<400>  6
tccaacccac ttatggtg                                                     18


<210>  7
<211>  82
<212>  DNA
<213>  Homo sapiens

<400>  7
ctcaggactt gttcttacct ttcttttcca atgttacttg gttccatgct atacatgtct       60

ctgggaccaa tggtactaag ag                                                82


<210>  8
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (B) Mutant del HV69/70

<400>  8
ctcaggactt gttcttacct ttcttttcca atgttacttg gttccatgct atctctggga       60

ccaatggtac taagag                                                       76


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (B)

<400>  9
ctcaggactt gttcttacc                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (B)

<400>  10
ctcttagtac cattggtcc                                                    19


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (B)

<400>  11
tccatgctat acatgtctct                                                   20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (B)

<400>  12
acttggttcc atgctatctc t                                                 21


<210>  13
<211>  72
<212>  DNA
<213>  Homo sapiens

<400>  13
ccaggaacaa atacttctaa ccaggttgct gttctttatc aggatgttaa ctgcacagaa       60

gtccctgttg ct                                                           72


<210>  14
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (C) Mutant D614G

<400>  14
ccaggaacaa atacttctaa ccaggttgct gttctttatc agggtgttaa ctgcacagaa       60

gtccctgttg ct                                                           72


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (C)

<400>  15
ccaggaacaa atacttctaa cc                                                22


<210>  16
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (C)

<400>  16
agcaacaggg acttctg                                                      17


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (C)

<400>  17
tgttctttat caggatgtta actg                                              24


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (C)

<400>  18
tgttctttat cagggtgtta act                                               23


<210>  19
<211>  74
<212>  DNA
<213>  Homo sapiens

<400>  19
tcagacaaat cgctccaggg caaactggaa agattgctga ttataattat aaattaccag       60

atgattttac aggc                                                         74


<210>  20
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (D) Mutant K417N

<400>  20
tcagacaaat cgctccaggg caaactggaa atattgctga ttataattat aaattaccag       60

atgattttac aggc                                                         74


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (D)

<400>  21
tcagacaaat cgctcca                                                      17


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (D)

<400>  22
gcctgtaaaa tcatctggta                                                   20


<210>  23
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (D)

<400>  23
caaactggaa agattgctg                                                    19


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (D)

<400>  24
caaactggaa atattgctg                                                    19


<210>  25
<211>  69
<212>  DNA
<213>  Homo sapiens

<400>  25
aggtatatgc gctagttatc agactcagac taattctcct cggcgggcac gtagtgtagc       60

tagtcaatc                                                               69


<210>  26
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (E) Mutant P681H

<400>  26
aggtatatgc gctagttatc agactcagac taattctcat cggcgggcac gtagtgtagc       60

tagtcaatc                                                               69


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (E)

<400>  27
aggtatatgc gctagttatc aga                                               23


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (E)

<400>  28
gattgactag ctacactacg t                                                 21


<210>  29
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (E)

<400>  29
actaattctc ctcggcg                                                      17


<210>  30
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (E)

<400>  30
actaattctc atcggcg                                                      17


<210>  31
<211>  80
<212>  DNA
<213>  Homo sapiens

<400>  31
cgcctattaa tttagtgcgt gatctccctc agggtttttc ggctttagaa ccattggtag       60

atttgccaat aggtattaac                                                   80


<210>  32
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (F) Mutant A222V

<400>  32
cgcctattaa tttagtgcgt gatctccctc agggtttttc ggttttagaa ccattggtag       60

atttgccaat aggtattaac                                                   80


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (F)

<400>  33
cgcctattaa tttagtgcgt                                                   20


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (F)

<400>  34
gttaatacct attggcaaat ctac                                              24


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (F)

<400>  35
agggtttttc ggctttagaa c                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (F)

<400>  36
agggtttttc ggttttagaa c                                                 21


<210>  37
<211>  82
<212>  DNA
<213>  Homo sapiens

<400>  37
agagagatat ttcaactgaa atctatcagg ccggtagcac accttgtaat ggtgttgaag       60

gttttaattg ttactttcct tt                                                82


<210>  38
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (G) Mutant S477N

<400>  38
agagagatat ttcaactgaa atctatcagg ccggtaacac accttgtaat ggtgttgaag       60

gttttaattg ttactttcct tt                                                82


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (G)

<400>  39
agagagatat ttcaactgaa atct                                              24


<210>  40
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (G)

<400>  40
aaaggaaagt aacaattaaa acct                                              24


<210>  41
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (G)

<400>  41
aggccggtag cacac                                                        15


<210>  42
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (G)

<400>  42
aggccggtaa cacac                                                        15


<210>  43
<211>  78
<212>  DNA
<213>  Homo sapiens

<400>  43
ccagatgttg atttaggtga catctctggc attaatgctt cagttgtaaa cattcaaaaa       60

gaaattgacc gcctcaat                                                     78


<210>  44
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (H) Mutant V1176F

<400>  44
ccagatgttg atttaggtga catctctggc attaatgctt catttgtaaa cattcaaaaa       60

gaaattgacc gcctcaat                                                     78


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (H)

<400>  45
ccagatgttg atttaggtga c                                                 21


<210>  46
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (H)

<400>  46
attgaggcgg tcaatttc                                                     18


<210>  47
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (H)

<400>  47
tggcattaat gcttcagttg taaa                                              24


<210>  48
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (H)

<400>  48
tggcattaat gcttcatttg taaa                                              24


<210>  49
<211>  77
<212>  DNA
<213>  Homo sapiens

<400>  49
agatgatttt acaggctgcg ttatagcttg gaattctaac aatcttgatt ctaaggttgg       60

tggtaattat aattacc                                                      77


<210>  50
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (I) Mutant N439K

<400>  50
agatgatttt acaggctgcg ttatagcttg gaattctaaa aatcttgatt ctaaggttgg       60

tggtaattat aattacc                                                      77


<210>  51
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (I)

<400>  51
agatgatttt acaggctgc                                                    19


<210>  52
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (I)

<400>  52
ggtaattata attaccacca acct                                              24


<210>  53
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (I)

<400>  53
tagcttggaa ttctaacaat cttga                                             25


<210>  54
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (I)

<400>  54
tagcttggaa ttctaaaaat cttga                                             25


<210>  55
<211>  94
<212>  DNA
<213>  Homo sapiens

<400>  55
agagagatat ttcaactgaa atctatcagg ccggtagcac accttgtaat ggtgttgaag       60

gttttaattg ttactttcct ttacaatcat atgg                                   94


<210>  56
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (J) Mutant E484K

<400>  56
agagagatat ttcaactgaa atctatcagg ccggtagcac accttgtaat ggtgttaaag       60

gttttaattg ttactttcct ttacaatcat atgg                                   94


<210>  57
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer (J)

<400>  57
agagagatat ttcaactgaa atct                                              24


<210>  58
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer (J)

<400>  58
ccatatgatt gtaaaggaaa gtaac                                             25


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype Probe (J)

<400>  59
ttgtaatggt gttgaaggtt tta                                               23


<210>  60
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant Probe (J)

<400>  60
ttgtaatggt gttaaaggtt tta                                               23


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N1 forward primer

<400>  61
gaccccaaaa tcagcgaaat                                                   20


<210>  62
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-n-CoV_N1 reverse primer

<400>  62
tctggttact gccagttgaa tctg                                              24


<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-n-CoV_N1 reverse probe

<400>  63
accccgcatt acgtttggtg gacc                                              24


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N2 forward primer

<400>  64
ttacaaacat tggccgcaaa                                                   20


<210>  65
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N2 reverse primer

<400>  65
gcgcgacatt ccgaagaa                                                     18


<210>  66
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N2 probe

<400>  66
acaatttgcc cccagcgctt cag                                               23


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N3 forward primer

<400>  67
gggagccttg aatacaccaa aa                                                22


<210>  68
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N3 reverse primer

<400>  68
tgtagcacga ttgcagcatt g                                                 21


<210>  69
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2019-nCoV_N3 probe

<400>  69
aycacattgg cacccgcaat cctg                                              24


<210>  70
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNAse P forward primer

<400>  70
agatttggac ctgcgagcg                                                    19


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNAse P reverse primer

<400>  71
gagcggctgt ctccacaagt                                                   20


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNAse P probe

<400>  72
ttctgacctg aaggctctgc gcg                                               23


<210>  73
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4 bp shortened on 3' end compared to patented wildtype probe

<400>  73
tccaacccac ttat                                                         14


<210>  74
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2 bp shortened on 3' end compared to patented wildtype probe

<400>  74
tccaacccac ttatgg                                                       16


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2 bp lengthened on 3' end compared to patented wildtype probe

<400>  75
tccaacccac ttatggtgtt                                                   20


<210>  76
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4 bp lengthened on 3' end compared to patented wildtype probe

<400>  76
tccaacccac ttatggtgtt gg                                                22


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target Sequence (K) Mutant E484K

<400>  77
accttgtaat ggtgttaaag gttt                                              24


