                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       University of Washington
 
<120>  SYSTEMS AND METHODS TO LINK CD40 SIGNALING TO ANTIGEN BINDING

<130>  F053-0124PCT / 20-143-WO-PCT / 49158.02WO2

<150>  US63/147,041
<151>  2021-02-08

<160>  203   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CIITA sgRNA

<400>  1
gauauuggca uaagccuccc                                                   20


<210>  2
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  B2M sgRNA

<400>  2
ggccgagaug ucucgcuccg                                                   20


<210>  3
<211>  2520
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD40 (humanCD40 signal peptide - anti-gp120 scFv - Linker - 
       StrepTagII - Gly6 Linker- full CD40 protein - Gly6 Linker - HA 
       tag - P2A skipping element- mCherry reporter)

<400>  3
caagtttaaa cggatctcta gcgatggtac ggctccctct gcaatgtgta ctttggggat       60

gtcttctgac tgccgtccat ccccaagtac agctcgtaca aagtggagct gaggtcaaaa      120

aaccgggtgc ttcagtgaaa gtctcatgtc aggctagtgg gtatagattt agtcatttta      180

cagttcactg ggttagacaa gcacctggac agaggtttga atggatgggt tggataaacc      240

catacaacgg taataaagag ttcagcgcga aatttcaaga ccgggtaact ttcacggctg      300

acacaagcgc taacaccgcg tacatggaac tgcgaagttt gaggagtgct gacaccgcag      360

tatactactg tgcacgcgtt ggcgagtggg gatgggatga ctcaccctac gacaattatt      420

atatggacgt ttggggtaaa ggcaccactg ttattgtcag ctcaggcggt ggagggtctg      480

gaggtggcgg ttccggtggt ggaggtagcg agatcgttct cacgcaagcc ccaggtacgc      540

tgtcactttc tcctggtgag cgagcaactt tcagttgtag atccagccat tccattcgca      600

gtagaagagt ggcctggtat caacacaagc cgggccaggc acctcgcctt gttattcacg      660

gggtgtcaaa ccgcgcttca gggatctcag atcgcttctc cggcagtggg agtggaaccg      720

acttcacttt gactatcact cgagttgaac ccgaggattt cgccttgtac tattgccaag      780

tttacggggc atcctcctat acatttggac agggcacaaa gctggagcgg aaaggatcag      840

cgggtagtgc agcagggagc ggcgagttct ggtctcatcc acaatttgaa aagggaggcg      900

gagggggtgg tgaaccgcca acagcttgca gggagaaaca ataccttatt aatagtcagt      960

gctgctcact gtgccagccg ggtcagaagc tggtcagcga ctgtacggaa ttcactgaaa     1020

cggagtgcct gccctgcggg gaaagtgaat tcttggatac gtggaatcga gaaactcatt     1080

gtcatcaaca caagtactgt gacccgaacc tgggtctgag ggtgcagcaa aaaggcacca     1140

gtgagacgga tacaatttgc acctgtgaag agggctggca ctgtacctcc gaggcgtgcg     1200

agagctgtgt tctccaccgc tcttgttctc ccggatttgg ggttaaacaa atagcaactg     1260

gggtttcaga tacaatatgc gaaccatgcc cggtcgggtt tttttcaaat gtctctagcg     1320

cgtttgaaaa gtgtcatcct tggacaagct gcgagactaa ggacctcgtc gttcagcaag     1380

cgggtacgaa taaaactgat gttgtttgtg ggcctcagga tcgacttaga gcactggttg     1440

tcatccctat tatatttggg attttgttcg ctatcctgct tgtgcttgta aagaaggttg     1500

ctaagaagcc cactaacaag gcgcctcacc cgaagcagga acctcaagaa attaattttc     1560

cagacgatct gccagggtca aacacggctg cacccgtaca ggaaacactg cacgggtgtc     1620

agcccgtgac tcaagaagac ggaaaagaga gccgaataag cgtgcaggag cgacaaggcg     1680

gtggcggcgg cgggtacccc tacgacgtgc cagattatgc aggctctggt gctactaatt     1740

tttctctgct gaaacaggca ggggacgtcg aagagaaccc tggccccatg gtttctaagg     1800

gggaagaaga caacatggca attatcaagg aatttatgcg atttaaggtg catatggaag     1860

gcagcgtgaa tggtcacgag ttcgaaatag aaggagaagg cgaaggtagg ccatacgaag     1920

gcacgcagac tgcgaaattg aaggtgacaa aagggggccc gctgccattt gcgtgggata     1980

ttttgagccc ccaattcatg tacggcagta aagcttacgt gaaacacccg gctgatatcc     2040

cagactatct gaagctttct tttccggagg gctttaagtg ggagcgggtt atgaattttg     2100

aagatggtgg agtcgtcaca gtaacccaag attccagttt gcaggacggt gaatttatat     2160

ataaggtgaa attgagggga acgaatttcc caagcgatgg acctgtaatg caaaaaaaaa     2220

caatggggtg ggaagcgtca tctgagcgca tgtatcccga ggatggcgca cttaaaggcg     2280

aaattaagca aagattgaaa ttgaaggacg gcggacacta tgacgctgaa gtaaagacta     2340

cttataaagc caaaaaaccg gtccaactcc ctggcgcgta taacgtgaac ataaagctgg     2400

acatcacaag ccataacgaa gactatacca ttgtagaaca gtatgaacgc gccgagggcc     2460

gccacagtac gggtggtatg gatgaactct acaagtgaag ggcccgtttc tgctagcaag     2520


<210>  4
<211>  824
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanCD40 signal peptide - anti-gp120 scFv - Linker - StrepTagII 
       - Gly6 Linker- full CD40 protein - Gly6 Linker - HA tag - P2A 
       skipping element- mCherry reporter

<400>  4

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg 
        35                  40                  45              


Phe Ser His Phe Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg 
    50                  55                  60                  


Phe Glu Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe 
65                  70                  75                  80  


Ser Ala Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala 
                85                  90                  95      


Asn Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro 
        115                 120                 125             


Tyr Asp Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Glu Ile Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser 
                165                 170                 175     


Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg 
            180                 185                 190         


Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg 
        195                 200                 205             


Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg 
225                 230                 235                 240 


Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala 
                245                 250                 255     


Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser 
            260                 265                 270         


Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe 
        275                 280                 285             


Glu Lys Gly Gly Gly Gly Gly Gly Glu Pro Pro Thr Ala Cys Arg Glu 
    290                 295                 300                 


Lys Gln Tyr Leu Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly 
305                 310                 315                 320 


Gln Lys Leu Val Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu 
                325                 330                 335     


Pro Cys Gly Glu Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His 
            340                 345                 350         


Cys His Gln His Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln 
        355                 360                 365             


Gln Lys Gly Thr Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly 
    370                 375                 380                 


Trp His Cys Thr Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser 
385                 390                 395                 400 


Cys Ser Pro Gly Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp 
                405                 410                 415     


Thr Ile Cys Glu Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser 
            420                 425                 430         


Ala Phe Glu Lys Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu 
        435                 440                 445             


Val Val Gln Gln Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro 
    450                 455                 460                 


Gln Asp Arg Leu Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile 
465                 470                 475                 480 


Leu Phe Ala Ile Leu Leu Val Leu Val Lys Lys Val Ala Lys Lys Pro 
                485                 490                 495     


Thr Asn Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe 
            500                 505                 510         


Pro Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr 
        515                 520                 525             


Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg 
    530                 535                 540                 


Ile Ser Val Gln Glu Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr 
545                 550                 555                 560 


Asp Val Pro Asp Tyr Ala Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu 
                565                 570                 575     


Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys 
            580                 585                 590         


Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys 
        595                 600                 605             


Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly 
    610                 615                 620                 


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
625                 630                 635                 640 


Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro 
                645                 650                 655     


Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile 
            660                 665                 670         


Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
        675                 680                 685             


Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser 
    690                 695                 700                 


Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr 
705                 710                 715                 720 


Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
                725                 730                 735     


Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly 
            740                 745                 750         


Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala 
        755                 760                 765             


Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly 
    770                 775                 780                 


Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp 
785                 790                 795                 800 


Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr 
                805                 810                 815     


Gly Gly Met Asp Glu Leu Tyr Lys 
            820                 


<210>  5
<211>  2085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG ECD (humanCD40 signal peptide - anti-gp120 scFv - Linker - 
       StrepTagII- Gly6 Linker- IgG Hinge- Gly6 Linker- CD40 
       Transmembrane Domain - CD40 Intracellular Domain - Gly6 Linker - 
       HA tag - P2A skipping element- mCherry reporter)

<400>  5
caagtttaaa cggatctcta gcgatggtac ggctccctct gcaatgtgta ctttggggat       60

gtcttctgac tgccgtccat ccccaagtac agctcgtaca aagtggagct gaggtcaaaa      120

aaccgggtgc ttcagtgaaa gtctcatgtc aggctagtgg gtatagattt agtcatttta      180

cagttcactg ggttagacaa gcacctggac agaggtttga atggatgggt tggataaacc      240

catacaacgg taataaagag ttcagcgcga aatttcaaga ccgggtaact ttcacggctg      300

acacaagcgc taacaccgcg tacatggaac tgcgaagttt gaggagtgct gacaccgcag      360

tatactactg tgcacgcgtt ggcgagtggg gatgggatga ctcaccctac gacaattatt      420

atatggacgt ttggggtaaa ggcaccactg ttattgtcag ctcaggcggt ggagggtctg      480

gaggtggcgg ttccggtggt ggaggtagcg agatcgttct cacgcaagcc ccaggtacgc      540

tgtcactttc tcctggtgag cgagcaactt tcagttgtag atccagccat tccattcgca      600

gtagaagagt ggcctggtat caacacaagc cgggccaggc acctcgcctt gttattcacg      660

gggtgtcaaa ccgcgcttca gggatctcag atcgcttctc cggcagtggg agtggaaccg      720

acttcacttt gactatcact cgagttgaac ccgaggattt cgccttgtac tattgccaag      780

tttacggggc atcctcctat acatttggac agggcacaaa gctggagcgg aaaggatcag      840

cgggtagtgc agcagggagc ggcgagttct ggtctcatcc acaatttgaa aagggaggcg      900

gaggtggagg tgaacccaag tcatgtgata agacacatac ttgtcccccc tgcccgggag      960

gtggtggagg gggtgctctc gtggttatcc ctatcatatt cggcatactt tttgcaatcc     1020

tccttgtcct ggtaaaaaag gtagcgaaaa agcccaagaa ggttgctaag aagcccacta     1080

acaaggcgcc tcacccgaag caggaacctc aagaaattaa ttttccagac gatctgccag     1140

ggtcaaacac ggctgcaccc gtacaggaaa cactgcacgg gtgtcagccc gtgactcaag     1200

aagacggaaa agagagccga ataagcgtgc aggagcgaca aggcggtggc ggcggcgggt     1260

acccctacga cgtgccagat tatgcaggct ctggtgctac taatttttct ctgctgaaac     1320

aggcagggga cgtcgaagag aaccctggcc ccatggtttc taagggggaa gaagacaaca     1380

tggcaattat caaggaattt atgcgattta aggtgcatat ggaaggcagc gtgaatggtc     1440

acgagttcga aatagaagga gaaggcgaag gtaggccata cgaaggcacg cagactgcga     1500

aattgaaggt gacaaaaggg ggcccgctgc catttgcgtg ggatattttg agcccccaat     1560

tcatgtacgg cagtaaagct tacgtgaaac acccggctga tatcccagac tatctgaagc     1620

tttcttttcc ggagggcttt aagtgggagc gggttatgaa ttttgaagat ggtggagtcg     1680

tcacagtaac ccaagattcc agtttgcagg acggtgaatt tatatataag gtgaaattga     1740

ggggaacgaa tttcccaagc gatggacctg taatgcaaaa aaaaacaatg gggtgggaag     1800

cgtcatctga gcgcatgtat cccgaggatg gcgcacttaa aggcgaaatt aagcaaagat     1860

tgaaattgaa ggacggcgga cactatgacg ctgaagtaaa gactacttat aaagccaaaa     1920

aaccggtcca actccctggc gcgtataacg tgaacataaa gctggacatc acaagccata     1980

acgaagacta taccattgta gaacagtatg aacgcgccga gggccgccac agtacgggtg     2040

gtatggatga actctacaag tgaagggccc gtttctgcta gcaag                     2085


<210>  6
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG ECD (humanCD40 signal peptide - anti-gp120 scFv - Linker - 
       StrepTagII- Gly6 Linker- IgG Hinge- Gly6 Linker- CD40 
       Transmembrane Domain - CD40 Intracellular Domain - Gly6 Linker - 
       HA tag - P2A skipping element- mCherry reporter)

<400>  6

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg 
        35                  40                  45              


Phe Ser His Phe Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg 
    50                  55                  60                  


Phe Glu Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe 
65                  70                  75                  80  


Ser Ala Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala 
                85                  90                  95      


Asn Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro 
        115                 120                 125             


Tyr Asp Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Glu Ile Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser 
                165                 170                 175     


Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg 
            180                 185                 190         


Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg 
        195                 200                 205             


Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg 
225                 230                 235                 240 


Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala 
                245                 250                 255     


Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser 
            260                 265                 270         


Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe 
        275                 280                 285             


Glu Lys Gly Gly Gly Gly Gly Gly Glu Pro Lys Ser Cys Asp Lys Thr 
    290                 295                 300                 


His Thr Cys Pro Pro Cys Pro Gly Gly Gly Gly Gly Gly Ala Leu Val 
305                 310                 315                 320 


Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu Leu Val Leu 
                325                 330                 335     


Val Lys Lys Val Ala Lys Lys Pro Lys Lys Val Ala Lys Lys Pro Thr 
            340                 345                 350         


Asn Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro 
        355                 360                 365             


Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu 
    370                 375                 380                 


His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile 
385                 390                 395                 400 


Ser Val Gln Glu Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp 
                405                 410                 415     


Val Pro Asp Tyr Ala Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys 
            420                 425                 430         


Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly 
        435                 440                 445             


Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val 
    450                 455                 460                 


His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
465                 470                 475                 480 


Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val 
                485                 490                 495     


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
            500                 505                 510         


Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro 
        515                 520                 525             


Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
    530                 535                 540                 


Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser 
545                 550                 555                 560 


Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn 
                565                 570                 575     


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
            580                 585                 590         


Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu 
        595                 600                 605             


Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu 
    610                 615                 620                 


Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala 
625                 630                 635                 640 


Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
                645                 650                 655     


Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly 
            660                 665                 670         


Gly Met Asp Glu Leu Tyr Lys 
        675                 


<210>  7
<211>  2082
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG ECD + TM (humanCD40 signal peptide - anti-gp120 scFv - Linker
       - StrepTagII- Gly6 Linker- IgG Hinge- Gly6 Linker- IgG2 
       Transmembrane Domain - Gly6 Linker - CD40 Intracellular Domain - 
       Gly6 Linker - HA tag - P2A skipping element- mCherry reporter)

<400>  7
caagtttaaa cggatctcta gcgatggtac ggctccctct gcaatgtgta ctttggggat       60

gtcttctgac tgccgtccat ccccaagtac agctcgtaca aagtggagct gaggtcaaaa      120

aaccgggtgc ttcagtgaaa gtctcatgtc aggctagtgg gtatagattt agtcatttta      180

cagttcactg ggttagacaa gcacctggac agaggtttga atggatgggt tggataaacc      240

catacaacgg taataaagag ttcagcgcga aatttcaaga ccgggtaact ttcacggctg      300

acacaagcgc taacaccgcg tacatggaac tgcgaagttt gaggagtgct gacaccgcag      360

tatactactg tgcacgcgtt ggcgagtggg gatgggatga ctcaccctac gacaattatt      420

atatggacgt ttggggtaaa ggcaccactg ttattgtcag ctcaggcggt ggagggtctg      480

gaggtggcgg ttccggtggt ggaggtagcg agatcgttct cacgcaagcc ccaggtacgc      540

tgtcactttc tcctggtgag cgagcaactt tcagttgtag atccagccat tccattcgca      600

gtagaagagt ggcctggtat caacacaagc cgggccaggc acctcgcctt gttattcacg      660

gggtgtcaaa ccgcgcttca gggatctcag atcgcttctc cggcagtggg agtggaaccg      720

acttcacttt gactatcact cgagttgaac ccgaggattt cgccttgtac tattgccaag      780

tttacggggc atcctcctat acatttggac agggcacaaa gctggagcgg aaaggatcag      840

cgggtagtgc agcagggagc ggcgagttct ggtctcatcc acaatttgaa aagggagggg      900

gaggcggagg agaaccaaaa agctgtgaca aaacgcacac ctgccctccc tgcccaggtc      960

tgtggactac tataaccatc tttatcacgc tttttttgct ttcagtttgc tatagcgcaa     1020

ctataacctt tttcgggggc ggcggtggtg gtaagaaggt tgctaagaag cccactaaca     1080

aggcgcctca cccgaagcag gaacctcaag aaattaattt tccagacgat ctgccagggt     1140

caaacacggc tgcacccgta caggaaacac tgcacgggtg tcagcccgtg actcaagaag     1200

acggaaaaga gagccgaata agcgtgcagg agcgacaagg cggtggcggc ggcgggtacc     1260

cctacgacgt gccagattat gcaggctctg gtgctactaa tttttctctg ctgaaacagg     1320

caggggacgt cgaagagaac cctggcccca tggtttctaa gggggaagaa gacaacatgg     1380

caattatcaa ggaatttatg cgatttaagg tgcatatgga aggcagcgtg aatggtcacg     1440

agttcgaaat agaaggagaa ggcgaaggta ggccatacga aggcacgcag actgcgaaat     1500

tgaaggtgac aaaagggggc ccgctgccat ttgcgtggga tattttgagc ccccaattca     1560

tgtacggcag taaagcttac gtgaaacacc cggctgatat cccagactat ctgaagcttt     1620

cttttccgga gggctttaag tgggagcggg ttatgaattt tgaagatggt ggagtcgtca     1680

cagtaaccca agattccagt ttgcaggacg gtgaatttat atataaggtg aaattgaggg     1740

gaacgaattt cccaagcgat ggacctgtaa tgcaaaaaaa aacaatgggg tgggaagcgt     1800

catctgagcg catgtatccc gaggatggcg cacttaaagg cgaaattaag caaagattga     1860

aattgaagga cggcggacac tatgacgctg aagtaaagac tacttataaa gccaaaaaac     1920

cggtccaact ccctggcgcg tataacgtga acataaagct ggacatcaca agccataacg     1980

aagactatac cattgtagaa cagtatgaac gcgccgaggg ccgccacagt acgggtggta     2040

tggatgaact ctacaagtga agggcccgtt tctgctagca ag                        2082


<210>  8
<211>  678
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG ECD + TM (humanCD40 signal peptide - anti-gp120 scFv - Linker
       - StrepTagII- Gly6 Linker- IgG Hinge- Gly6 Linker- IgG2 
       Transmembrane Domain - Gly6 Linker - CD40 Intracellular Domain - 
       Gly6 Linker - HA tag - P2A skipping element- mCherry reporter)

<400>  8

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg 
        35                  40                  45              


Phe Ser His Phe Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg 
    50                  55                  60                  


Phe Glu Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe 
65                  70                  75                  80  


Ser Ala Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala 
                85                  90                  95      


Asn Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro 
        115                 120                 125             


Tyr Asp Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Glu Ile Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser 
                165                 170                 175     


Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg 
            180                 185                 190         


Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg 
        195                 200                 205             


Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg 
225                 230                 235                 240 


Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala 
                245                 250                 255     


Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser 
            260                 265                 270         


Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe 
        275                 280                 285             


Glu Lys Gly Gly Gly Gly Gly Gly Glu Pro Lys Ser Cys Asp Lys Thr 
    290                 295                 300                 


His Thr Cys Pro Pro Cys Pro Gly Leu Trp Thr Thr Ile Thr Ile Phe 
305                 310                 315                 320 


Ile Thr Leu Phe Leu Leu Ser Val Cys Tyr Ser Ala Thr Ile Thr Phe 
                325                 330                 335     


Phe Gly Gly Gly Gly Gly Gly Lys Lys Val Ala Lys Lys Pro Thr Asn 
            340                 345                 350         


Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp 
        355                 360                 365             


Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His 
    370                 375                 380                 


Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser 
385                 390                 395                 400 


Val Gln Glu Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val 
                405                 410                 415     


Pro Asp Tyr Ala Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
            420                 425                 430         


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu 
        435                 440                 445             


Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His 
    450                 455                 460                 


Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly 
465                 470                 475                 480 


Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr 
                485                 490                 495     


Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe 
            500                 505                 510         


Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp 
        515                 520                 525             


Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met 
    530                 535                 540                 


Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu 
545                 550                 555                 560 


Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe 
                565                 570                 575     


Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala 
            580                 585                 590         


Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile 
        595                 600                 605             


Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val 
    610                 615                 620                 


Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr 
625                 630                 635                 640 


Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr 
                645                 650                 655     


Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly 
            660                 665                 670         


Met Asp Glu Leu Tyr Lys 
        675             


<210>  9
<211>  3225
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  full CD40+FKBP12v36 (humanCD40 signal peptide - anti-gp120 scFv -
       Linker - StrepTagII - Gly6 Linker- full CD40 protein - Gly6 
       Linker- FkBP12v36 - GlySer Linker - FkBP12v36- Gly6 Linker - HA 
       tag - P2A skipping element- mCherry reporter)

<400>  9
caagtttaaa cggatctcta gcgatggtac ggctccctct gcaatgtgta ctttggggat       60

gtcttctgac tgccgtccat ccccaagtac agctcgtaca aagtggagct gaggtcaaaa      120

aaccgggtgc ttcagtgaaa gtctcatgtc aggctagtgg gtatagattt agtcatttta      180

cagttcactg ggttagacaa gcacctggac agaggtttga atggatgggt tggataaacc      240

catacaacgg taataaagag ttcagcgcga aatttcaaga ccgggtaact ttcacggctg      300

acacaagcgc taacaccgcg tacatggaac tgcgaagttt gaggagtgct gacaccgcag      360

tatactactg tgcacgcgtt ggcgagtggg gatgggatga ctcaccctac gacaattatt      420

atatggacgt ttggggtaaa ggcaccactg ttattgtcag ctcaggcggt ggagggtctg      480

gaggtggcgg ttccggtggt ggaggtagcg agatcgttct cacgcaagcc ccaggtacgc      540

tgtcactttc tcctggtgag cgagcaactt tcagttgtag atccagccat tccattcgca      600

gtagaagagt ggcctggtat caacacaagc cgggccaggc acctcgcctt gttattcacg      660

gggtgtcaaa ccgcgcttca gggatctcag atcgcttctc cggcagtggg agtggaaccg      720

acttcacttt gactatcact cgagttgaac ccgaggattt cgccttgtac tattgccaag      780

tttacggggc atcctcctat acatttggac agggcacaaa gctggagcgg aaaggatcag      840

cgggtagtgc agcagggagc ggcgagttct ggtctcatcc acaatttgaa aagggaggcg      900

gagggggtgg tgaaccgcca acagcttgca gggagaaaca ataccttatt aatagtcagt      960

gctgctcact gtgccagccg ggtcagaagc tggtcagcga ctgtacggaa ttcactgaaa     1020

cggagtgcct gccctgcggg gaaagtgaat tcttggatac gtggaatcga gaaactcatt     1080

gtcatcaaca caagtactgt gacccgaacc tgggtctgag ggtgcagcaa aaaggcacca     1140

gtgagacgga tacaatttgc acctgtgaag agggctggca ctgtacctcc gaggcgtgcg     1200

agagctgtgt tctccaccgc tcttgttctc ccggatttgg ggttaaacaa atagcaactg     1260

gggtttcaga tacaatatgc gaaccatgcc cggtcgggtt tttttcaaat gtctctagcg     1320

cgtttgaaaa gtgtcatcct tggacaagct gcgagactaa ggacctcgtc gttcagcaag     1380

cgggtacgaa taaaactgat gttgtttgtg ggcctcagga tcgacttaga gcactggttg     1440

tcatccctat tatatttggg attttgttcg ctatcctgct tgtgcttgta aagaaggttg     1500

ctaagaagcc cactaacaag gcgcctcacc cgaagcagga acctcaagaa attaattttc     1560

cagacgatct gccagggtca aacacggctg cacccgtaca ggaaacactg cacgggtgtc     1620

agcccgtgac tcaagaagac ggaaaagaga gccgaataag cgtgcaggag cgacaaggag     1680

gtggcggagg aggcggggtg caagtagaaa ccatatctcc tggtgacgga cggacgtttc     1740

ccaagcgagg acaaacgtgc gtggtacact acaccgggat gctggaagac gggaaaaaag     1800

ttgatagcag cagggaccga aataagccat tcaaatttat gctgggaaag caggaagtga     1860

tccgaggctg ggaagaagga gttgcccaga tgtcagtagg ccagcgggct aaactgacaa     1920

tctcacccga ctatgcatac ggggcaacgg gacacccggg aattattcct cctcacgcta     1980

cgctggtctt tgacgttgaa ttgttgaaac tggagggcgg gggcggatca ggtggtggtg     2040

gatcaggcgg aggtggaagt ggggttcaag tggaaacaat ttctccggga gatggcagaa     2100

cctttcccaa acgcggccag acctgcgtgg tacattacac tggcatgctg gaggatggta     2160

aaaaagtaga ctcaagccga gacaggaaca agccttttaa atttatgttg ggcaaacaag     2220

aggttattcg cgggtgggaa gaaggggtcg cgcagatgtc cgtcggccaa cgagctaaac     2280

tcacgataag tccggattat gcatatgggg ctaccggaca cccaggcatt atcccacccc     2340

acgcgacgct tgtctttgat gttgaacttt tgaaattgga aggcggcggg ggaggtggct     2400

acccctacga cgtgccagat tatgcaggct ctggtgctac taatttttct ctgctgaaac     2460

aggcagggga cgtcgaagag aaccctggcc ccatggtttc taagggggaa gaagacaaca     2520

tggcaattat caaggaattt atgcgattta aggtgcatat ggaaggcagc gtgaatggtc     2580

acgagttcga aatagaagga gaaggcgaag gtaggccata cgaaggcacg cagactgcga     2640

aattgaaggt gacaaaaggg ggcccgctgc catttgcgtg ggatattttg agcccccaat     2700

tcatgtacgg cagtaaagct tacgtgaaac acccggctga tatcccagac tatctgaagc     2760

tttcttttcc ggagggcttt aagtgggagc gggttatgaa ttttgaagat ggtggagtcg     2820

tcacagtaac ccaagattcc agtttgcagg acggtgaatt tatatataag gtgaaattga     2880

ggggaacgaa tttcccaagc gatggacctg taatgcaaaa aaaaacaatg gggtgggaag     2940

cgtcatctga gcgcatgtat cccgaggatg gcgcacttaa aggcgaaatt aagcaaagat     3000

tgaaattgaa ggacggcgga cactatgacg ctgaagtaaa gactacttat aaagccaaaa     3060

aaccggtcca actccctggc gcgtataacg tgaacataaa gctggacatc acaagccata     3120

acgaagacta taccattgta gaacagtatg aacgcgccga gggccgccac agtacgggtg     3180

gtatggatga actctacaag tgaagggccc gtttctgcta gcaag                     3225


<210>  10
<211>  1059
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  full CD40+FKBP12v36 (humanCD40 signal peptide - anti-gp120 scFv -
       Linker - StrepTagII - Gly6 Linker- full CD40 protein - Gly6 
       Linker- FkBP12v36 - GlySer Linker - FkBP12v36- Gly6 Linker - HA 
       tag - P2A skipping element- mCherry reporter)

<400>  10

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg 
        35                  40                  45              


Phe Ser His Phe Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg 
    50                  55                  60                  


Phe Glu Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe 
65                  70                  75                  80  


Ser Ala Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala 
                85                  90                  95      


Asn Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro 
        115                 120                 125             


Tyr Asp Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Glu Ile Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser 
                165                 170                 175     


Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg 
            180                 185                 190         


Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg 
        195                 200                 205             


Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg 
225                 230                 235                 240 


Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala 
                245                 250                 255     


Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser 
            260                 265                 270         


Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe 
        275                 280                 285             


Glu Lys Gly Gly Gly Gly Gly Gly Glu Pro Pro Thr Ala Cys Arg Glu 
    290                 295                 300                 


Lys Gln Tyr Leu Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly 
305                 310                 315                 320 


Gln Lys Leu Val Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu 
                325                 330                 335     


Pro Cys Gly Glu Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His 
            340                 345                 350         


Cys His Gln His Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln 
        355                 360                 365             


Gln Lys Gly Thr Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly 
    370                 375                 380                 


Trp His Cys Thr Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser 
385                 390                 395                 400 


Cys Ser Pro Gly Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp 
                405                 410                 415     


Thr Ile Cys Glu Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser 
            420                 425                 430         


Ala Phe Glu Lys Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu 
        435                 440                 445             


Val Val Gln Gln Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro 
    450                 455                 460                 


Gln Asp Arg Leu Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile 
465                 470                 475                 480 


Leu Phe Ala Ile Leu Leu Val Leu Val Lys Lys Val Ala Lys Lys Pro 
                485                 490                 495     


Thr Asn Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe 
            500                 505                 510         


Pro Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr 
        515                 520                 525             


Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg 
    530                 535                 540                 


Ile Ser Val Gln Glu Arg Gln Gly Gly Gly Gly Gly Gly Gly Val Gln 
545                 550                 555                 560 


Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly 
                565                 570                 575     


Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys 
            580                 585                 590         


Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly 
        595                 600                 605             


Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser 
    610                 615                 620                 


Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly 
625                 630                 635                 640 


Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe 
                645                 650                 655     


Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly 
            660                 665                 670         


Gly Ser Gly Gly Gly Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro 
        675                 680                 685             


Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
    690                 695                 700                 


Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp 
705                 710                 715                 720 


Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
                725                 730                 735     


Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
            740                 745                 750         


Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
        755                 760                 765             


Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
    770                 775                 780                 


Leu Glu Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp Tyr 
785                 790                 795                 800 


Ala Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
                805                 810                 815     


Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Asp Asn 
            820                 825                 830         


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
        835                 840                 845             


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
    850                 855                 860                 


Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
865                 870                 875                 880 


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
                885                 890                 895     


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
            900                 905                 910         


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
        915                 920                 925             


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
    930                 935                 940                 


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
945                 950                 955                 960 


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu 
                965                 970                 975     


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
            980                 985                 990         


Leu Lys Leu Lys Asp Gly Gly His  Tyr Asp Ala Glu Val  Lys Thr Thr 
        995                 1000                 1005             


Tyr Lys  Ala Lys Lys Pro Val  Gln Leu Pro Gly Ala  Tyr Asn Val 
    1010                 1015                 1020             


Asn Ile  Lys Leu Asp Ile Thr  Ser His Asn Glu Asp  Tyr Thr Ile 
    1025                 1030                 1035             


Val Glu  Gln Tyr Glu Arg Ala  Glu Gly Arg His Ser  Thr Gly Gly 
    1040                 1045                 1050             


Met Asp  Glu Leu Tyr Lys 
    1055                 


<210>  11
<211>  1692
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  full CD40 no scFv (human CD40 signal peptide - full CD40 protein 
       - Gly6 Linker - HA tag - P2A skipping element- mCherry Reporter)

<400>  11
caagtttaaa cggatctcta gcgatggtac ggctccctct gcaatgtgta ctttggggat       60

gtcttctgac tgccgtccat cccgaaccgc caacagcttg cagggagaaa caatacctta      120

ttaatagtca gtgctgctca ctgtgccagc cgggtcagaa gctggtcagc gactgtacgg      180

aattcactga aacggagtgc ctgccctgcg gggaaagtga attcttggat acgtggaatc      240

gagaaactca ttgtcatcaa cacaagtact gtgacccgaa cctgggtctg agggtgcagc      300

aaaaaggcac cagtgagacg gatacaattt gcacctgtga agagggctgg cactgtacct      360

ccgaggcgtg cgagagctgt gttctccacc gctcttgttc tcccggattt ggggttaaac      420

aaatagcaac tggggtttca gatacaatat gcgaaccatg cccggtcggg tttttttcaa      480

atgtctctag cgcgtttgaa aagtgtcatc cttggacaag ctgcgagact aaggacctcg      540

tcgttcagca agcgggtacg aataaaactg atgttgtttg tgggcctcag gatcgactta      600

gagcactggt tgtcatccct attatatttg ggattttgtt cgctatcctg cttgtgcttg      660

taaagaaggt tgctaagaag cccactaaca aggcgcctca cccgaagcag gaacctcaag      720

aaattaattt tccagacgat ctgccagggt caaacacggc tgcacccgta caggaaacac      780

tgcacgggtg tcagcccgtg actcaagaag acggaaaaga gagccgaata agcgtgcagg      840

agcgacaagg cggtggcggc ggcgggtacc cctacgacgt gccagattat gcaggctctg      900

gtgctactaa tttttctctg ctgaaacagg caggggacgt cgaagagaac cctggcccca      960

tggtttctaa gggggaagaa gacaacatgg caattatcaa ggaatttatg cgatttaagg     1020

tgcatatgga aggcagcgtg aatggtcacg agttcgaaat agaaggagaa ggcgaaggta     1080

ggccatacga aggcacgcag actgcgaaat tgaaggtgac aaaagggggc ccgctgccat     1140

ttgcgtggga tattttgagc ccccaattca tgtacggcag taaagcttac gtgaaacacc     1200

cggctgatat cccagactat ctgaagcttt cttttccgga gggctttaag tgggagcggg     1260

ttatgaattt tgaagatggt ggagtcgtca cagtaaccca agattccagt ttgcaggacg     1320

gtgaatttat atataaggtg aaattgaggg gaacgaattt cccaagcgat ggacctgtaa     1380

tgcaaaaaaa aacaatgggg tgggaagcgt catctgagcg catgtatccc gaggatggcg     1440

cacttaaagg cgaaattaag caaagattga aattgaagga cggcggacac tatgacgctg     1500

aagtaaagac tacttataaa gccaaaaaac cggtccaact ccctggcgcg tataacgtga     1560

acataaagct ggacatcaca agccataacg aagactatac cattgtagaa cagtatgaac     1620

gcgccgaggg ccgccacagt acgggtggta tggatgaact ctacaagtga agggcccgtt     1680

tctgctagca ag                                                         1692


<210>  12
<211>  548
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  full CD40 no scFv (human CD40 signal peptide - full CD40 protein 
       - Gly6 Linker - HA tag - P2A skipping element- mCherry Reporter)

<400>  12

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu 
            20                  25                  30          


Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val 
        35                  40                  45              


Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu 
    50                  55                  60                  


Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His 
65                  70                  75                  80  


Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr 
                85                  90                  95      


Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr 
            100                 105                 110         


Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly 
        115                 120                 125             


Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu 
    130                 135                 140                 


Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys 
145                 150                 155                 160 


Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln 
                165                 170                 175     


Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu 
            180                 185                 190         


Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile 
        195                 200                 205             


Leu Leu Val Leu Val Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala 
    210                 215                 220                 


Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu 
225                 230                 235                 240 


Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
                245                 250                 255     


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
            260                 265                 270         


Glu Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp 
        275                 280                 285             


Tyr Ala Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
    290                 295                 300                 


Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Asp 
305                 310                 315                 320 


Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu 
                325                 330                 335     


Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly 
            340                 345                 350         


Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly 
        355                 360                 365             


Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr 
    370                 375                 380                 


Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu 
385                 390                 395                 400 


Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe 
                405                 410                 415     


Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp 
            420                 425                 430         


Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser 
        435                 440                 445             


Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser 
    450                 455                 460                 


Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln 
465                 470                 475                 480 


Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr 
                485                 490                 495     


Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val 
            500                 505                 510         


Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val 
        515                 520                 525             


Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp 
    530                 535                 540                 


Glu Leu Tyr Lys 
545             


<210>  13
<211>  7736
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MRT3_PTT3_mouseCD79a_IntracellularCD40 
       (mouseCD79alpha-mouseIntracellularCD40-P2A skipping element-T2A 
       skipping element-eGFP Reporter)

<400>  13
agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat       60

aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt      120

atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat      180

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      240

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      300

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      360

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc      420

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac      480

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc      540

ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc      600

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa      660

aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg      720

tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg      780

tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt      840

actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc      900

gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg      960

caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg     1020

gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg     1080

aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat     1140

tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc     1200

cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt     1260

ccactcccag gtccaagttt aaacggatct ctagcgatgc ctggtggcct ggaagccctg     1320

cgcgccctgc ccttgttgct ttttctctct tacgcttgcc ttggtccagg ttgtcaagca     1380

ctcagggtcg aaggagggcc ccccagcctc acagtgaatc tcggtgagga agcccggttg     1440

acctgtgaga acaacggtag aaaccccaac atcacctggt ggttttcttt gcaatcaaac     1500

atcacctggc ctcctgtccc actgggcccc ggccaaggaa caactggaca actgttcttt     1560

cccgaggtga ataagaacca ccgaggactc tattggtgcc aagttataga gaacaacatc     1620

cttaagcgaa gctgcgggac ttatcttagg gttcgcaacc ctgtccccag accattcctt     1680

gacatggggg aagggaccaa gaatcgaata atcacagcag agggtattat tctgttgttt     1740

tgcgctgtgg tgccaggtac acttctgctt tttcggaagc ggtggcaaaa cgagaagttc     1800

ggcgtggata tgcctgacga ttacgaggat gagaacctgt acgaggggct taatctggat     1860

gattgtagta tgtacgaaga catcagcaga ggcctgcaag ggacatacca agacgtaggg     1920

aacctgcaca tcggtgatgc tcaactggag aaaccaaaga aagtggtcaa gaagccaaag     1980

gacaatgaga ttctgccccc cgctgcaaga aggcaagatc cccaggagat ggaggactat     2040

ccaggccaca acacagccgc cccagtgcag gaaactctgc acggttgcca acctgttaca     2100

caggaggatg ggaaggaaag ccgcatcagt gtgcaagagc gacaagtgac agactcaata     2160

gccttgcggc ccctcgttgg ctccggcgct accaatttta gcctgttgaa gcaggctggg     2220

gacgtggaag agaaccccgg accaggaagt ggcgagggaa gggggagtct tcttacctgt     2280

ggggatgttg aagagaatcc tggccccatg gtatccaagg gagaggagct gttcacaggc     2340

gttgtcccaa ttctcgtgga attggatggg gacgtaaatg gtcacaagtt tagcgtctct     2400

ggcgagggcg agggtgacgc aacatatggt aaattgactc tgaagtttat ttgcaccaca     2460

ggtaaacttc ctgtgccttg gcctacactt gtcactactc tgacctatgg cgtgcaatgt     2520

ttctcccgct accctgacca tatgaaacaa cacgattttt ttaaatctgc catgcccgaa     2580

gggtatgtgc aggaacggac tattttcttt aaggacgacg gcaattataa aactcgagct     2640

gaggtcaaat ttgaaggtga caccttggtc aacagaatag aactcaaggg cattgatttt     2700

aaagaggacg ggaacatact gggtcacaaa ttggagtaca attataactc ccacaacgtt     2760

tatataatgg ctgataagca gaaaaatgga attaaggtca acttcaaaat ccgacataac     2820

atagaggatg ggtcagtaca gcttgccgac cattatcagc aaaacactcc tataggcgat     2880

ggacccgtgc ttttgcctga caatcattat ctgtctactc aaagcgctct ttctaaagat     2940

cctaacgaaa aacgggatca tatggtgctc ttggagtttg taactgccgc cggcatcaca     3000

ttgggaatgg acgaactcta taaatgaccc tctagagggc ccgtttctgc tagcaagctt     3060

gctagcggcc gctcgaggcc ggcaaggccg gatcccccga cctcgacctc tggctaataa     3120

aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg     3180

acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg     3240

atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca     3300

gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt     3360

gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat     3420

ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt     3480

ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc     3540

tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat     3600

cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt     3660

tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata     3720

ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg     3780

aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca     3840

cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg     3900

gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc     3960

attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga     4020

ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca     4080

gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac     4140

tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca     4200

agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc     4260

tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga     4320

accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg     4380

actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc     4440

cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca     4500

gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga     4560

ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc     4620

cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct     4680

ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata     4740

ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca     4800

aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc     4860

tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt     4920

tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc     4980

tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata     5040

gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata     5100

tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata     5160

tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata     5220

tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata     5280

tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta     5340

tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac     5400

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt     5460

agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     5520

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     5580

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     5640

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     5700

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     5760

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     5820

gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact     5880

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     5940

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     6000

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     6060

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     6120

agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg     6180

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     6240

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     6300

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     6360

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     6420

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     6480

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     6540

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     6600

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     6660

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     6720

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc     6780

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     6840

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     6900

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     6960

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     7020

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     7080

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     7140

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     7200

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     7260

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     7320

ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag     7380

tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     7440

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg     7500

caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg     7560

ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc     7620

atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc     7680

gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggc         7736


<210>  14
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRT3_PTT3_mouseCD79a_IntracellularCD40 
       (mouseCD79alpha-mouseIntracellularCD40-P2A skipping element-T2A 
       skipping element-eGFP Reporter)

<400>  14

Met Pro Gly Gly Leu Glu Ala Leu Arg Ala Leu Pro Leu Leu Leu Phe 
1               5                   10                  15      


Leu Ser Tyr Ala Cys Leu Gly Pro Gly Cys Gln Ala Leu Arg Val Glu 
            20                  25                  30          


Gly Gly Pro Pro Ser Leu Thr Val Asn Leu Gly Glu Glu Ala Arg Leu 
        35                  40                  45              


Thr Cys Glu Asn Asn Gly Arg Asn Pro Asn Ile Thr Trp Trp Phe Ser 
    50                  55                  60                  


Leu Gln Ser Asn Ile Thr Trp Pro Pro Val Pro Leu Gly Pro Gly Gln 
65                  70                  75                  80  


Gly Thr Thr Gly Gln Leu Phe Phe Pro Glu Val Asn Lys Asn His Arg 
                85                  90                  95      


Gly Leu Tyr Trp Cys Gln Val Ile Glu Asn Asn Ile Leu Lys Arg Ser 
            100                 105                 110         


Cys Gly Thr Tyr Leu Arg Val Arg Asn Pro Val Pro Arg Pro Phe Leu 
        115                 120                 125             


Asp Met Gly Glu Gly Thr Lys Asn Arg Ile Ile Thr Ala Glu Gly Ile 
    130                 135                 140                 


Ile Leu Leu Phe Cys Ala Val Val Pro Gly Thr Leu Leu Leu Phe Arg 
145                 150                 155                 160 


Lys Arg Trp Gln Asn Glu Lys Phe Gly Val Asp Met Pro Asp Asp Tyr 
                165                 170                 175     


Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn Leu Asp Asp Cys Ser Met 
            180                 185                 190         


Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly Thr Tyr Gln Asp Val Gly 
        195                 200                 205             


Asn Leu His Ile Gly Asp Ala Gln Leu Glu Lys Pro Lys Lys Val Val 
    210                 215                 220                 


Lys Lys Pro Lys Asp Asn Glu Ile Leu Pro Pro Ala Ala Arg Arg Gln 
225                 230                 235                 240 


Asp Pro Gln Glu Met Glu Asp Tyr Pro Gly His Asn Thr Ala Ala Pro 
                245                 250                 255     


Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly 
            260                 265                 270         


Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln Val Thr Asp Ser Ile 
        275                 280                 285             


Ala Leu Arg Pro Leu Val Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu 
    290                 295                 300                 


Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Gly Ser Gly Glu 
305                 310                 315                 320 


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
                325                 330                 335     


Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
            340                 345                 350         


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
        355                 360                 365             


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
    370                 375                 380                 


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
385                 390                 395                 400 


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
                405                 410                 415     


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
            420                 425                 430         


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
        435                 440                 445             


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
    450                 455                 460                 


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
465                 470                 475                 480 


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
                485                 490                 495     


Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
            500                 505                 510         


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
        515                 520                 525             


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
    530                 535                 540                 


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
545                 550                 555                 560 


Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                565                 570                 575     


<210>  15
<211>  7760
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MRT4_PTT3_mouseCD79b_IntracellularCD40 
       (mouseCD79beta-mouseIntracelluarCD40-P2A skipping element-T2A 
       skipping element- eGFP Reporter)

<400>  15
agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat       60

aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt      120

atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat      180

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      240

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      300

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      360

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc      420

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac      480

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc      540

ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc      600

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa      660

aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg      720

tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg      780

tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt      840

actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc      900

gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg      960

caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg     1020

gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg     1080

aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat     1140

tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc     1200

cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt     1260

ccactcccag gtccaagttt aaacggatct ctagcgatgg ctacactggt tctcagctca     1320

atgccatgtc attggctttt gtttttgctg ttgctgttca gtggggagcc agtgcctgct     1380

atgacatcct ccgatttgcc cttgaacttt cagggatcac cctgcagcca aatatggcag     1440

cacccacgct ttgcagctaa aaagcgctcc agcatggtca aatttcattg ttatacaaac     1500

cacagcgggg cattgacctg gttcagaaag aggggcagcc aacagcctca agaattggtc     1560

agtgaggaag gtcgcatagt gcagacacaa aatggctccg tatataccct cactatccaa     1620

aacattcagt acgaagacaa cggaatctat ttttgtaagc agaagtgcga cagtgccaat     1680

cacaatgtaa cagatagctg tggcaccgag cttctggttc ttggcttctc aacacttgat     1740

cagttgaagc gacgaaatac cctgaaagac ggtattatac tcattcaaac tttgctcata     1800

atcctgttca taatagtacc tatcttcctg ctccttgata aggacgacgg gaaagcaggc     1860

atggaagaag atcacaccta cgaggggttg aatatcgacc agacagccac ctacgaagac     1920

attgtgactc tccgaaccgg ggaggtgaaa tggagtgtag gagaacaccc cggtcaagaa     1980

aagaaagtgg tcaagaagcc aaaggacaat gagattctgc cccccgctgc aagaaggcaa     2040

gatccccagg agatggagga ctatccaggc cacaacacag ccgccccagt gcaggaaact     2100

ctgcacggtt gccaacctgt tacacaggag gatgggaagg aaagccgcat cagtgtgcaa     2160

gagcgacaag tgacagactc aatagccttg cggcccctcg ttggctccgg cgctaccaat     2220

tttagcctgt tgaagcaggc tggggacgtg gaagagaacc ccggaccagg aagtggcgag     2280

ggaaggggga gtcttcttac ctgtggggat gttgaagaga atcctggccc catggtatcc     2340

aagggagagg agctgttcac aggcgttgtc ccaattctcg tggaattgga tggggacgta     2400

aatggtcaca agtttagcgt ctctggcgag ggcgagggtg acgcaacata tggtaaattg     2460

actctgaagt ttatttgcac cacaggtaaa cttcctgtgc cttggcctac acttgtcact     2520

actctgacct atggcgtgca atgtttctcc cgctaccctg accatatgaa acaacacgat     2580

ttttttaaat ctgccatgcc cgaagggtat gtgcaggaac ggactatttt ctttaaggac     2640

gacggcaatt ataaaactcg agctgaggtc aaatttgaag gtgacacctt ggtcaacaga     2700

atagaactca agggcattga ttttaaagag gacgggaaca tactgggtca caaattggag     2760

tacaattata actcccacaa cgtttatata atggctgata agcagaaaaa tggaattaag     2820

gtcaacttca aaatccgaca taacatagag gatgggtcag tacagcttgc cgaccattat     2880

cagcaaaaca ctcctatagg cgatggaccc gtgcttttgc ctgacaatca ttatctgtct     2940

actcaaagcg ctctttctaa agatcctaac gaaaaacggg atcatatggt gctcttggag     3000

tttgtaactg ccgccggcat cacattggga atggacgaac tctataaatg accctctaga     3060

gggcccgttt ctgctagcaa gcttgctagc ggccgctcga ggccggcaag gccggatccc     3120

ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat     3180

tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct     3240

cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca     3300

tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg     3360

ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg     3420

actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat     3480

cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct     3540

tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact     3600

acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct     3660

caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat     3720

atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta     3780

cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga     3840

tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg     3900

tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc     3960

tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag     4020

ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag     4080

aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc     4140

acaaacccct tgggcaataa atactagtgt aggggtgacg cccccagaat aaaatttgga     4200

cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg     4260

gcaataaata ctagtgtagg atcctagtgc aatatgatac tggggttatt aagatgtgtc     4320

ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa     4380

gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa     4440

acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa     4500

attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa     4560

ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg     4620

cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag     4680

ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca     4740

agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt     4800

gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc     4860

tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc     4920

tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc     4980

tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc     5040

cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc     5100

tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg     5160

tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg     5220

tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg     5280

tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg     5340

tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc     5400

aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta     5460

atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg     5520

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     5580

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     5640

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     5700

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     5760

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     5820

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag     5880

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     5940

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     6000

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     6060

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     6120

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa     6180

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     6240

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     6300

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     6360

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     6420

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     6480

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat     6540

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg     6600

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc     6660

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg     6720

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag     6780

cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact     6840

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg     6900

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc     6960

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg     7020

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg     7080

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag     7140

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc     7200

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct     7260

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc     7320

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc     7380

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac     7440

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact     7500

ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc     7560

aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat     7620

ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat     7680

tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc     7740

gcagaactgg taggtatggc                                                 7760


<210>  16
<211>  584
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRT4_PTT3_mouseCD79b_IntracellularCD40 
       (mouseCD79beta-mouseIntracelluarCD40-P2A skipping element-T2A 
       skipping element- eGFP Reporter)

<400>  16

Met Ala Thr Leu Val Leu Ser Ser Met Pro Cys His Trp Leu Leu Phe 
1               5                   10                  15      


Leu Leu Leu Leu Phe Ser Gly Glu Pro Val Pro Ala Met Thr Ser Ser 
            20                  25                  30          


Asp Leu Pro Leu Asn Phe Gln Gly Ser Pro Cys Ser Gln Ile Trp Gln 
        35                  40                  45              


His Pro Arg Phe Ala Ala Lys Lys Arg Ser Ser Met Val Lys Phe His 
    50                  55                  60                  


Cys Tyr Thr Asn His Ser Gly Ala Leu Thr Trp Phe Arg Lys Arg Gly 
65                  70                  75                  80  


Ser Gln Gln Pro Gln Glu Leu Val Ser Glu Glu Gly Arg Ile Val Gln 
                85                  90                  95      


Thr Gln Asn Gly Ser Val Tyr Thr Leu Thr Ile Gln Asn Ile Gln Tyr 
            100                 105                 110         


Glu Asp Asn Gly Ile Tyr Phe Cys Lys Gln Lys Cys Asp Ser Ala Asn 
        115                 120                 125             


His Asn Val Thr Asp Ser Cys Gly Thr Glu Leu Leu Val Leu Gly Phe 
    130                 135                 140                 


Ser Thr Leu Asp Gln Leu Lys Arg Arg Asn Thr Leu Lys Asp Gly Ile 
145                 150                 155                 160 


Ile Leu Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro Ile 
                165                 170                 175     


Phe Leu Leu Leu Asp Lys Asp Asp Gly Lys Ala Gly Met Glu Glu Asp 
            180                 185                 190         


His Thr Tyr Glu Gly Leu Asn Ile Asp Gln Thr Ala Thr Tyr Glu Asp 
        195                 200                 205             


Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu His 
    210                 215                 220                 


Pro Gly Gln Glu Lys Lys Val Val Lys Lys Pro Lys Asp Asn Glu Ile 
225                 230                 235                 240 


Leu Pro Pro Ala Ala Arg Arg Gln Asp Pro Gln Glu Met Glu Asp Tyr 
                245                 250                 255     


Pro Gly His Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            260                 265                 270         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        275                 280                 285             


Glu Arg Gln Val Thr Asp Ser Ile Ala Leu Arg Pro Leu Val Gly Ser 
    290                 295                 300                 


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
305                 310                 315                 320 


Asn Pro Gly Pro Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys 
                325                 330                 335     


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu 
            340                 345                 350         


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
        355                 360                 365             


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
    370                 375                 380                 


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
385                 390                 395                 400 


Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys 
                405                 410                 415     


Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
            420                 425                 430         


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
        435                 440                 445             


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
    450                 455                 460                 


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
465                 470                 475                 480 


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
                485                 490                 495     


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
            500                 505                 510         


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
        515                 520                 525             


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
    530                 535                 540                 


His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
545                 550                 555                 560 


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
                565                 570                 575     


Leu Gly Met Asp Glu Leu Tyr Lys 
            580                 


<210>  17
<211>  7638
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MRT5_Ptt3_humanCD79a_IntracellularCD40 
       (humanCD79alpha-humanIntracellularCD40-P2A skipping 
       element-mCherry Reporter)

<400>  17
agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat       60

aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt      120

atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat      180

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      240

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      300

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      360

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc      420

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac      480

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc      540

ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc      600

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa      660

aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg      720

tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg      780

tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt      840

actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc      900

gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg      960

caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg     1020

gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg     1080

aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat     1140

tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc     1200

cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt     1260

ccactcccag gtccaagttt aaacggatct ctagcgatgc cgggtggacc cggtgtcttg     1320

caagccctcc ccgctaccat ctttcttttg tttctcctct cagcagttta tttgggtccc     1380

ggctgccaag ccctctggat gcacaaagtc ccggcctctc ttatggtatc tcttggtgag     1440

gatgcgcact ttcagtgtcc acataactcc tctaataatg ctaacgttac atggtggagg     1500

gtccttcacg gaaattatac ttggcctcca gagtttttgg ggcctggcga ggatccaaat     1560

ggaacgctga ttatacagaa cgtgaataaa tctcacggag ggatctatgt ttgtcgagtt     1620

caagagggga atgaatcata tcagcaaagc tgcgggactt atttgcgggt acgacaacca     1680

ccacctagac cattcctgga tatgggagaa ggaaccaaaa atagaattat cacagctgag     1740

ggcataatcc tcctcttttg tgcagtagtt ccaggtaccc tcctgctctt tagaaagcga     1800

tggcaaaatg agaaactcgg gttggatgct ggagatgaat atgaggatga gaatctgtac     1860

gaaggtctga atctggatga ttgtagtatg tacgaggata tttcaagagg tctgcaaggt     1920

acctatcaag atgtcggctc cttgaatatc ggcgacgtac agctcgagaa accgaaaaaa     1980

gtggctaaga aaccaactaa taaagctccc caccccaaac aggagccaca agagataaat     2040

ttcccagatg accttccagg atcaaacaca gcagcaccag ttcaagaaac actgcacggc     2100

tgccaacctg ttacacagga agacgggaaa gagagcagaa tttccgtcca agaacgacag     2160

ggctctggtg ctactaattt ttctctgctg aaacaggcag gggacgtcga agagaaccct     2220

ggccccatgg tttctaaggg ggaagaagac aacatggcaa ttatcaagga atttatgcga     2280

tttaaggtgc atatggaagg cagcgtgaat ggtcacgagt tcgaaataga aggagaaggc     2340

gaaggtaggc catacgaagg cacgcagact gcgaaattga aggtgacaaa agggggcccg     2400

ctgccatttg cgtgggatat tttgagcccc caattcatgt acggcagtaa agcttacgtg     2460

aaacacccgg ctgatatccc agactatctg aagctttctt ttccggaggg ctttaagtgg     2520

gagcgggtta tgaattttga agatggtgga gtcgtcacag taacccaaga ttccagtttg     2580

caggacggtg aatttatata taaggtgaaa ttgaggggaa cgaatttccc aagcgatgga     2640

cctgtaatgc aaaaaaaaac aatggggtgg gaagcgtcat ctgagcgcat gtatcccgag     2700

gatggcgcac ttaaaggcga aattaagcaa agattgaaat tgaaggacgg cggacactat     2760

gacgctgaag taaagactac ttataaagcc aaaaaaccgg tccaactccc tggcgcgtat     2820

aacgtgaaca taaagctgga catcacaagc cataacgaag actataccat tgtagaacag     2880

tatgaacgcg ccgagggccg ccacagtacg ggtggtatgg atgaactcta caagtgaagg     2940

gcccgtttct gctagcaagc ttgctagcgg ccgctcgagg ccggcaaggc cggatccccc     3000

gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt     3060

tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg     3120

gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc     3180

tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc     3240

aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac     3300

tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc     3360

tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg     3420

gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac     3480

gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca     3540

agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat     3600

gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc     3660

caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata     3720

tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta     3780

gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc     3840

ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt     3900

gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa     3960

taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac     4020

aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat     4080

agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat     4140

ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc     4200

aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga     4260

gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac     4320

ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat     4380

tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat     4440

aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc     4500

cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat     4560

aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag     4620

cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc     4680

catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg     4740

ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg     4800

ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg     4860

ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg     4920

ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg     4980

ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta     5040

gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta     5100

gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta     5160

gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta     5220

gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa     5280

acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat     5340

gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga     5400

acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa     5460

ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt     5520

gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg     5580

ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg     5640

gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg     5700

agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag     5760

caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca     5820

gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg     5880

agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc     5940

gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg     6000

aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg     6060

ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac     6120

tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg     6180

tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg     6240

gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact     6300

atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa     6360

ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt     6420

aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag     6480

ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct     6540

ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt     6600

tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg     6660

cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct     6720

gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc     6780

gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg     6840

tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa     6900

ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg     6960

gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg     7020

ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga     7080

tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt     7140

ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct     7200

gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga     7260

acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg     7320

cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg     7380

aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag     7440

gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt     7500

cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc     7560

tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc     7620

agaactggta ggtatggc                                                   7638


<210>  18
<211>  547
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRT5_Ptt3_humanCD79a_IntracellularCD40 
       (humanCD79alpha-humanIntracellularCD40-P2A skipping 
       element-mCherry Reporter)

<400>  18

Met Pro Gly Gly Pro Gly Val Leu Gln Ala Leu Pro Ala Thr Ile Phe 
1               5                   10                  15      


Leu Leu Phe Leu Leu Ser Ala Val Tyr Leu Gly Pro Gly Cys Gln Ala 
            20                  25                  30          


Leu Trp Met His Lys Val Pro Ala Ser Leu Met Val Ser Leu Gly Glu 
        35                  40                  45              


Asp Ala His Phe Gln Cys Pro His Asn Ser Ser Asn Asn Ala Asn Val 
    50                  55                  60                  


Thr Trp Trp Arg Val Leu His Gly Asn Tyr Thr Trp Pro Pro Glu Phe 
65                  70                  75                  80  


Leu Gly Pro Gly Glu Asp Pro Asn Gly Thr Leu Ile Ile Gln Asn Val 
                85                  90                  95      


Asn Lys Ser His Gly Gly Ile Tyr Val Cys Arg Val Gln Glu Gly Asn 
            100                 105                 110         


Glu Ser Tyr Gln Gln Ser Cys Gly Thr Tyr Leu Arg Val Arg Gln Pro 
        115                 120                 125             


Pro Pro Arg Pro Phe Leu Asp Met Gly Glu Gly Thr Lys Asn Arg Ile 
    130                 135                 140                 


Ile Thr Ala Glu Gly Ile Ile Leu Leu Phe Cys Ala Val Val Pro Gly 
145                 150                 155                 160 


Thr Leu Leu Leu Phe Arg Lys Arg Trp Gln Asn Glu Lys Leu Gly Leu 
                165                 170                 175     


Asp Ala Gly Asp Glu Tyr Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn 
            180                 185                 190         


Leu Asp Asp Cys Ser Met Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly 
        195                 200                 205             


Thr Tyr Gln Asp Val Gly Ser Leu Asn Ile Gly Asp Val Gln Leu Glu 
    210                 215                 220                 


Lys Pro Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro 
225                 230                 235                 240 


Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser 
                245                 250                 255     


Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val 
            260                 265                 270         


Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Glu Ser Val Gln Glu Arg 
        275                 280                 285             


Gln Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
    290                 295                 300                 


Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Asp Asn 
305                 310                 315                 320 


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
                325                 330                 335     


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
            340                 345                 350         


Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
        355                 360                 365             


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
    370                 375                 380                 


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
385                 390                 395                 400 


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
                405                 410                 415     


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
            420                 425                 430         


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
        435                 440                 445             


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu 
    450                 455                 460                 


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
465                 470                 475                 480 


Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr 
                485                 490                 495     


Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn 
            500                 505                 510         


Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu 
        515                 520                 525             


Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu 
    530                 535                 540                 


Leu Tyr Lys 
545         


<210>  19
<211>  7647
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MRT6_Ptt3_humanCD79b_IntracellularCD40 
       (humanCD79beta-humanIntracellularCD40-P2A skipping 
       element-mCherry Reporter)

<400>  19
agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat       60

aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt      120

atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat      180

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      240

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      300

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      360

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc      420

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac      480

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc      540

ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc      600

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa      660

aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg      720

tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg      780

tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt      840

actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc      900

gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg      960

caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg     1020

gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg     1080

aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat     1140

tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc     1200

cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt     1260

ccactcccag gtccaagttt aaacggatct ctagcgatgg cccgactcgc actgtcacca     1320

gtaccgtcac attggatggt ggcattgttg cttcttctga gtgctgaacc cgtcccagcc     1380

gctcggagtg aggatcggta ccggaatccg aaaggctcag catgttcccg aatctggcaa     1440

tcacctaggt ttattgctag aaaacgcggc ttcacagtca aaatgcactg ctacatgaac     1500

agcgcaagtg gtaacgttag ctggctttgg aagcaggaga tggacgaaaa cccgcaacag     1560

ctcaaattgg aaaagggacg catggaggaa tcccaaaatg aatcactggc aacacttacg     1620

atccaaggta ttagattcga ggataacgga atctacttct gtcagcagaa atgcaataat     1680

acgagcgaag tctatcaagg ctgtggtaca gagctgagag tgatggggtt tagcactctg     1740

gcccaactta aacaaagaaa caccttgaag gacggaataa tcatgatcca aactttgctc     1800

atcattcttt ttatcatagt gcctattttc ttgttgctcg ataaggacga cagtaaagcg     1860

ggaatggaag aagatcatac atatgagggc cttgacattg accaaactgc gacgtatgaa     1920

gacatagtta ccttgcggac gggcgaagtt aaatggagtg ttggcgagca tcctggtcag     1980

gaaaaaaaag tggctaagaa accaactaat aaagctcccc accccaaaca ggagccacaa     2040

gagataaatt tcccagatga ccttccagga tcaaacacag cagcaccagt tcaagaaaca     2100

ctgcacggct gccaacctgt tacacaggaa gacgggaaag agagcagaat ttccgtccaa     2160

gaacgacagg gctctggtgc tactaatttt tctctgctga aacaggcagg ggacgtcgaa     2220

gagaaccctg gccccatggt ttctaagggg gaagaagaca acatggcaat tatcaaggaa     2280

tttatgcgat ttaaggtgca tatggaaggc agcgtgaatg gtcacgagtt cgaaatagaa     2340

ggagaaggcg aaggtaggcc atacgaaggc acgcagactg cgaaattgaa ggtgacaaaa     2400

gggggcccgc tgccatttgc gtgggatatt ttgagccccc aattcatgta cggcagtaaa     2460

gcttacgtga aacacccggc tgatatccca gactatctga agctttcttt tccggagggc     2520

tttaagtggg agcgggttat gaattttgaa gatggtggag tcgtcacagt aacccaagat     2580

tccagtttgc aggacggtga atttatatat aaggtgaaat tgaggggaac gaatttccca     2640

agcgatggac ctgtaatgca aaaaaaaaca atggggtggg aagcgtcatc tgagcgcatg     2700

tatcccgagg atggcgcact taaaggcgaa attaagcaaa gattgaaatt gaaggacggc     2760

ggacactatg acgctgaagt aaagactact tataaagcca aaaaaccggt ccaactccct     2820

ggcgcgtata acgtgaacat aaagctggac atcacaagcc ataacgaaga ctataccatt     2880

gtagaacagt atgaacgcgc cgagggccgc cacagtacgg gtggtatgga tgaactctac     2940

aagtgaaggg cccgtttctg ctagcaagct tgctagcggc cgctcgaggc cggcaaggcc     3000

ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg     3060

ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga     3120

gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac     3180

tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta     3240

caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg     3300

ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg     3360

ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg     3420

taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag     3480

gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc     3540

ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg     3600

gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca     3660

tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga     3720

acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc     3780

acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag     3840

tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac     3900

tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg     3960

cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat     4020

aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc     4080

tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca     4140

cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag     4200

atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa     4260

ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga     4320

aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt     4380

ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga     4440

ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa     4500

ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg     4560

ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg     4620

agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc     4680

taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat     4740

ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat     4800

ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat     4860

ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat     4920

ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat     4980

ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat     5040

atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat     5100

atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat     5160

atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat     5220

atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata     5280

agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta     5340

taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat     5400

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     5460

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     5520

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     5580

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     5640

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     5700

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc     5760

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     5820

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     5880

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     5940

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     6000

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg     6060

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     6120

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     6180

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     6240

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     6300

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     6360

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     6420

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6480

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6540

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6600

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6660

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     6720

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     6780

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     6840

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     6900

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     6960

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     7020

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     7080

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     7140

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     7200

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     7260

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     7320

cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt     7380

cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag     7440

gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga     7500

taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc     7560

gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc     7620

tgcaggcgca gaactggtag gtatggc                                         7647


<210>  20
<211>  549
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRT6_Ptt3_humanCD79b_IntracellularCD40 
       (humanCD79beta-humanIntracellularCD40-P2A skipping 
       element-mCherry Reporter)

<400>  20

Met Ala Arg Leu Ala Leu Ser Pro Val Pro Ser His Trp Met Val Ala 
1               5                   10                  15      


Leu Leu Leu Leu Leu Ser Ala Glu Pro Val Pro Ala Ala Arg Ser Glu 
            20                  25                  30          


Asp Arg Tyr Arg Asn Pro Lys Gly Ser Ala Cys Ser Arg Ile Trp Gln 
        35                  40                  45              


Ser Pro Arg Phe Ile Ala Arg Lys Arg Gly Phe Thr Val Lys Met His 
    50                  55                  60                  


Cys Tyr Met Asn Ser Ala Ser Gly Asn Val Ser Trp Leu Trp Lys Gln 
65                  70                  75                  80  


Glu Met Asp Glu Asn Pro Gln Gln Leu Lys Leu Glu Lys Gly Arg Met 
                85                  90                  95      


Glu Glu Ser Gln Asn Glu Ser Leu Ala Thr Leu Thr Ile Gln Gly Ile 
            100                 105                 110         


Arg Phe Glu Asp Asn Gly Ile Tyr Phe Cys Gln Gln Lys Cys Asn Asn 
        115                 120                 125             


Thr Ser Glu Val Tyr Gln Gly Cys Gly Thr Glu Leu Arg Val Met Gly 
    130                 135                 140                 


Phe Ser Thr Leu Ala Gln Leu Lys Gln Arg Asn Thr Leu Lys Asp Gly 
145                 150                 155                 160 


Ile Ile Met Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro 
                165                 170                 175     


Ile Phe Leu Leu Leu Asp Lys Asp Asp Ser Lys Ala Gly Met Glu Glu 
            180                 185                 190         


Asp His Thr Tyr Glu Gly Leu Asp Ile Asp Gln Thr Ala Thr Tyr Glu 
        195                 200                 205             


Asp Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu 
    210                 215                 220                 


His Pro Gly Gln Glu Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala 
225                 230                 235                 240 


Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu 
                245                 250                 255     


Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            260                 265                 270         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        275                 280                 285             


Glu Arg Gln Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
    290                 295                 300                 


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu 
305                 310                 315                 320 


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
                325                 330                 335     


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
            340                 345                 350         


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
        355                 360                 365             


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
    370                 375                 380                 


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
385                 390                 395                 400 


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
                405                 410                 415     


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
            420                 425                 430         


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
        435                 440                 445             


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
    450                 455                 460                 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
465                 470                 475                 480 


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
                485                 490                 495     


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
            500                 505                 510         


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
        515                 520                 525             


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
    530                 535                 540                 


Asp Glu Leu Tyr Lys 
545                 


<210>  21
<211>  546
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-gp120 scFv - Linker - StrepTagII - Gly6 Linker- full CD40 
       protein - Gly6 Linker - HA tag

<400>  21

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser His Phe 
            20                  25                  30          


Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala Lys Phe 
    50                  55                  60                  


Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro Tyr Asp Asn Tyr 
            100                 105                 110         


Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
    130                 135                 140                 


Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg 
145                 150                 155                 160 


Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg Ser Arg Arg Val 
                165                 170                 175     


Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg Leu Val Ile His 
            180                 185                 190         


Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg Val Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala Ser Ser Tyr Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser Ala Gly Ser Ala 
                245                 250                 255     


Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            260                 265                 270         


Gly Gly Gly Gly Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu 
        275                 280                 285             


Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val 
    290                 295                 300                 


Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu 
305                 310                 315                 320 


Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His 
                325                 330                 335     


Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr 
            340                 345                 350         


Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr 
        355                 360                 365             


Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly 
    370                 375                 380                 


Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu 
385                 390                 395                 400 


Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys 
                405                 410                 415     


Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln 
            420                 425                 430         


Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu 
        435                 440                 445             


Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile 
    450                 455                 460                 


Leu Leu Val Leu Val Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala 
465                 470                 475                 480 


Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu 
                485                 490                 495     


Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            500                 505                 510         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        515                 520                 525             


Glu Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp 
    530                 535                 540                 


Tyr Ala 
545     


<210>  22
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-gp120 scFv - Linker - StrepTagII- Gly6 Linker- IgG Hinge- 
       Gly6 Linker- CD40 Transmembrane Domain - CD40 Intracellular 
       Domain - Gly6 Linker - HA tag

<400>  22

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser His Phe 
            20                  25                  30          


Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala Lys Phe 
    50                  55                  60                  


Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro Tyr Asp Asn Tyr 
            100                 105                 110         


Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
    130                 135                 140                 


Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg 
145                 150                 155                 160 


Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg Ser Arg Arg Val 
                165                 170                 175     


Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg Leu Val Ile His 
            180                 185                 190         


Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg Val Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala Ser Ser Tyr Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser Ala Gly Ser Ala 
                245                 250                 255     


Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            260                 265                 270         


Gly Gly Gly Gly Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
        275                 280                 285             


Pro Cys Pro Gly Gly Gly Gly Gly Gly Ala Leu Val Val Ile Pro Ile 
    290                 295                 300                 


Ile Phe Gly Ile Leu Phe Ala Ile Leu Leu Val Leu Val Lys Lys Val 
305                 310                 315                 320 


Ala Lys Lys Pro Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro 
                325                 330                 335     


His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro 
            340                 345                 350         


Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln 
        355                 360                 365             


Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu 
    370                 375                 380                 


Arg Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp Tyr 
385                 390                 395                 400 


Ala 
    


<210>  23
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-gp120 scFv - Linker - StrepTagII- Gly6 Linker- IgG Hinge- 
       Gly6 Linker- IgG2 Transmembrane Domain - Gly6 Linker - CD40 
       Intracellular Domain - Gly6 Linker - HA tag

<400>  23

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser His Phe 
            20                  25                  30          


Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala Lys Phe 
    50                  55                  60                  


Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro Tyr Asp Asn Tyr 
            100                 105                 110         


Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
    130                 135                 140                 


Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg 
145                 150                 155                 160 


Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg Ser Arg Arg Val 
                165                 170                 175     


Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg Leu Val Ile His 
            180                 185                 190         


Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg Val Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala Ser Ser Tyr Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser Ala Gly Ser Ala 
                245                 250                 255     


Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            260                 265                 270         


Gly Gly Gly Gly Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
        275                 280                 285             


Pro Cys Pro Gly Leu Trp Thr Thr Ile Thr Ile Phe Ile Thr Leu Phe 
    290                 295                 300                 


Leu Leu Ser Val Cys Tyr Ser Ala Thr Ile Thr Phe Phe Gly Gly Gly 
305                 310                 315                 320 


Gly Gly Gly Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His 
                325                 330                 335     


Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly 
            340                 345                 350         


Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro 
        355                 360                 365             


Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg 
    370                 375                 380                 


Gln Gly Gly Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
385                 390                 395                 400 


<210>  24
<211>  782
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-gp120 scFv - Linker - StrepTagII - Gly6 Linker- full CD40 
       protein - Gly6 Linker- FkBP12v36 - GlySer Linker - FkBP12v36- 
       Gly6 Linker - HA tag

<400>  24

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser His Phe 
            20                  25                  30          


Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala Lys Phe 
    50                  55                  60                  


Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro Tyr Asp Asn Tyr 
            100                 105                 110         


Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
    130                 135                 140                 


Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg 
145                 150                 155                 160 


Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg Ser Arg Arg Val 
                165                 170                 175     


Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg Leu Val Ile His 
            180                 185                 190         


Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg Val Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala Ser Ser Tyr Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser Ala Gly Ser Ala 
                245                 250                 255     


Ala Gly Ser Gly Glu Phe Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            260                 265                 270         


Gly Gly Gly Gly Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu 
        275                 280                 285             


Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val 
    290                 295                 300                 


Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu 
305                 310                 315                 320 


Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His 
                325                 330                 335     


Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr 
            340                 345                 350         


Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr 
        355                 360                 365             


Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly 
    370                 375                 380                 


Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu 
385                 390                 395                 400 


Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys 
                405                 410                 415     


Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln 
            420                 425                 430         


Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu 
        435                 440                 445             


Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile 
    450                 455                 460                 


Leu Leu Val Leu Val Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala 
465                 470                 475                 480 


Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu 
                485                 490                 495     


Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            500                 505                 510         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        515                 520                 525             


Glu Arg Gln Gly Gly Gly Gly Gly Gly Gly Val Gln Val Glu Thr Ile 
    530                 535                 540                 


Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val 
545                 550                 555                 560 


Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser 
                565                 570                 575     


Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val 
            580                 585                 590         


Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg 
        595                 600                 605             


Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His 
    610                 615                 620                 


Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu 
625                 630                 635                 640 


Leu Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                645                 650                 655     


Gly Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
            660                 665                 670         


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
        675                 680                 685             


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
    690                 695                 700                 


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
705                 710                 715                 720 


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
                725                 730                 735     


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
            740                 745                 750         


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly 
        755                 760                 765             


Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly 
    770                 775                 780         


<210>  25
<211>  294
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MouseCD79alpha-IntracellularCD40

<400>  25

Met Pro Gly Gly Leu Glu Ala Leu Arg Ala Leu Pro Leu Leu Leu Phe 
1               5                   10                  15      


Leu Ser Tyr Ala Cys Leu Gly Pro Gly Cys Gln Ala Leu Arg Val Glu 
            20                  25                  30          


Gly Gly Pro Pro Ser Leu Thr Val Asn Leu Gly Glu Glu Ala Arg Leu 
        35                  40                  45              


Thr Cys Glu Asn Asn Gly Arg Asn Pro Asn Ile Thr Trp Trp Phe Ser 
    50                  55                  60                  


Leu Gln Ser Asn Ile Thr Trp Pro Pro Val Pro Leu Gly Pro Gly Gln 
65                  70                  75                  80  


Gly Thr Thr Gly Gln Leu Phe Phe Pro Glu Val Asn Lys Asn His Arg 
                85                  90                  95      


Gly Leu Tyr Trp Cys Gln Val Ile Glu Asn Asn Ile Leu Lys Arg Ser 
            100                 105                 110         


Cys Gly Thr Tyr Leu Arg Val Arg Asn Pro Val Pro Arg Pro Phe Leu 
        115                 120                 125             


Asp Met Gly Glu Gly Thr Lys Asn Arg Ile Ile Thr Ala Glu Gly Ile 
    130                 135                 140                 


Ile Leu Leu Phe Cys Ala Val Val Pro Gly Thr Leu Leu Leu Phe Arg 
145                 150                 155                 160 


Lys Arg Trp Gln Asn Glu Lys Phe Gly Val Asp Met Pro Asp Asp Tyr 
                165                 170                 175     


Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn Leu Asp Asp Cys Ser Met 
            180                 185                 190         


Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly Thr Tyr Gln Asp Val Gly 
        195                 200                 205             


Asn Leu His Ile Gly Asp Ala Gln Leu Glu Lys Pro Lys Lys Val Val 
    210                 215                 220                 


Lys Lys Pro Lys Asp Asn Glu Ile Leu Pro Pro Ala Ala Arg Arg Gln 
225                 230                 235                 240 


Asp Pro Gln Glu Met Glu Asp Tyr Pro Gly His Asn Thr Ala Ala Pro 
                245                 250                 255     


Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly 
            260                 265                 270         


Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln Val Thr Asp Ser Ile 
        275                 280                 285             


Ala Leu Arg Pro Leu Val 
    290                 


<210>  26
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MouseCD79beta-IntracelluarCD40

<400>  26

Met Ala Thr Leu Val Leu Ser Ser Met Pro Cys His Trp Leu Leu Phe 
1               5                   10                  15      


Leu Leu Leu Leu Phe Ser Gly Glu Pro Val Pro Ala Met Thr Ser Ser 
            20                  25                  30          


Asp Leu Pro Leu Asn Phe Gln Gly Ser Pro Cys Ser Gln Ile Trp Gln 
        35                  40                  45              


His Pro Arg Phe Ala Ala Lys Lys Arg Ser Ser Met Val Lys Phe His 
    50                  55                  60                  


Cys Tyr Thr Asn His Ser Gly Ala Leu Thr Trp Phe Arg Lys Arg Gly 
65                  70                  75                  80  


Ser Gln Gln Pro Gln Glu Leu Val Ser Glu Glu Gly Arg Ile Val Gln 
                85                  90                  95      


Thr Gln Asn Gly Ser Val Tyr Thr Leu Thr Ile Gln Asn Ile Gln Tyr 
            100                 105                 110         


Glu Asp Asn Gly Ile Tyr Phe Cys Lys Gln Lys Cys Asp Ser Ala Asn 
        115                 120                 125             


His Asn Val Thr Asp Ser Cys Gly Thr Glu Leu Leu Val Leu Gly Phe 
    130                 135                 140                 


Ser Thr Leu Asp Gln Leu Lys Arg Arg Asn Thr Leu Lys Asp Gly Ile 
145                 150                 155                 160 


Ile Leu Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro Ile 
                165                 170                 175     


Phe Leu Leu Leu Asp Lys Asp Asp Gly Lys Ala Gly Met Glu Glu Asp 
            180                 185                 190         


His Thr Tyr Glu Gly Leu Asn Ile Asp Gln Thr Ala Thr Tyr Glu Asp 
        195                 200                 205             


Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu His 
    210                 215                 220                 


Pro Gly Gln Glu Lys Lys Val Val Lys Lys Pro Lys Asp Asn Glu Ile 
225                 230                 235                 240 


Leu Pro Pro Ala Ala Arg Arg Gln Asp Pro Gln Glu Met Glu Asp Tyr 
                245                 250                 255     


Pro Gly His Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            260                 265                 270         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        275                 280                 285             


Glu Arg Gln Val Thr Asp Ser Ile Ala Leu Arg Pro Leu Val 
    290                 295                 300         


<210>  27
<211>  289
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HumanCD79alpha-IntracellularCD40

<400>  27

Met Pro Gly Gly Pro Gly Val Leu Gln Ala Leu Pro Ala Thr Ile Phe 
1               5                   10                  15      


Leu Leu Phe Leu Leu Ser Ala Val Tyr Leu Gly Pro Gly Cys Gln Ala 
            20                  25                  30          


Leu Trp Met His Lys Val Pro Ala Ser Leu Met Val Ser Leu Gly Glu 
        35                  40                  45              


Asp Ala His Phe Gln Cys Pro His Asn Ser Ser Asn Asn Ala Asn Val 
    50                  55                  60                  


Thr Trp Trp Arg Val Leu His Gly Asn Tyr Thr Trp Pro Pro Glu Phe 
65                  70                  75                  80  


Leu Gly Pro Gly Glu Asp Pro Asn Gly Thr Leu Ile Ile Gln Asn Val 
                85                  90                  95      


Asn Lys Ser His Gly Gly Ile Tyr Val Cys Arg Val Gln Glu Gly Asn 
            100                 105                 110         


Glu Ser Tyr Gln Gln Ser Cys Gly Thr Tyr Leu Arg Val Arg Gln Pro 
        115                 120                 125             


Pro Pro Arg Pro Phe Leu Asp Met Gly Glu Gly Thr Lys Asn Arg Ile 
    130                 135                 140                 


Ile Thr Ala Glu Gly Ile Ile Leu Leu Phe Cys Ala Val Val Pro Gly 
145                 150                 155                 160 


Thr Leu Leu Leu Phe Arg Lys Arg Trp Gln Asn Glu Lys Leu Gly Leu 
                165                 170                 175     


Asp Ala Gly Asp Glu Tyr Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn 
            180                 185                 190         


Leu Asp Asp Cys Ser Met Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly 
        195                 200                 205             


Thr Tyr Gln Asp Val Gly Ser Leu Asn Ile Gly Asp Val Gln Leu Glu 
    210                 215                 220                 


Lys Pro Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro 
225                 230                 235                 240 


Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser 
                245                 250                 255     


Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val 
            260                 265                 270         


Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Glu Ser Val Gln Glu Arg 
        275                 280                 285             


Gln 
    


<210>  28
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HumanCD79beta-IntracellularCD40

<400>  28

Met Ala Arg Leu Ala Leu Ser Pro Val Pro Ser His Trp Met Val Ala 
1               5                   10                  15      


Leu Leu Leu Leu Leu Ser Ala Glu Pro Val Pro Ala Ala Arg Ser Glu 
            20                  25                  30          


Asp Arg Tyr Arg Asn Pro Lys Gly Ser Ala Cys Ser Arg Ile Trp Gln 
        35                  40                  45              


Ser Pro Arg Phe Ile Ala Arg Lys Arg Gly Phe Thr Val Lys Met His 
    50                  55                  60                  


Cys Tyr Met Asn Ser Ala Ser Gly Asn Val Ser Trp Leu Trp Lys Gln 
65                  70                  75                  80  


Glu Met Asp Glu Asn Pro Gln Gln Leu Lys Leu Glu Lys Gly Arg Met 
                85                  90                  95      


Glu Glu Ser Gln Asn Glu Ser Leu Ala Thr Leu Thr Ile Gln Gly Ile 
            100                 105                 110         


Arg Phe Glu Asp Asn Gly Ile Tyr Phe Cys Gln Gln Lys Cys Asn Asn 
        115                 120                 125             


Thr Ser Glu Val Tyr Gln Gly Cys Gly Thr Glu Leu Arg Val Met Gly 
    130                 135                 140                 


Phe Ser Thr Leu Ala Gln Leu Lys Gln Arg Asn Thr Leu Lys Asp Gly 
145                 150                 155                 160 


Ile Ile Met Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro 
                165                 170                 175     


Ile Phe Leu Leu Leu Asp Lys Asp Asp Ser Lys Ala Gly Met Glu Glu 
            180                 185                 190         


Asp His Thr Tyr Glu Gly Leu Asp Ile Asp Gln Thr Ala Thr Tyr Glu 
        195                 200                 205             


Asp Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu 
    210                 215                 220                 


His Pro Gly Gln Glu Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala 
225                 230                 235                 240 


Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu 
                245                 250                 255     


Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys 
            260                 265                 270         


Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln 
        275                 280                 285             


Glu Arg Gln 
    290     


<210>  29
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  29

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro 
            20  


<210>  30
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-gp120 scFv

<400>  30

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser His Phe 
            20                  25                  30          


Thr Val His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala Lys Phe 
    50                  55                  60                  


Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Val Gly Glu Trp Gly Trp Asp Asp Ser Pro Tyr Asp Asn Tyr 
            100                 105                 110         


Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
    130                 135                 140                 


Val Leu Thr Gln Ala Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg 
145                 150                 155                 160 


Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg Ser Arg Arg Val 
                165                 170                 175     


Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg Leu Val Ile His 
            180                 185                 190         


Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg Val Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala Ser Ser Tyr Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys Gly Ser Ala Gly Ser Ala 
                245                 250                 255     


Ala Gly Ser Gly Glu Phe 
            260         


<210>  31
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40

<400>  31

Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu Ile Asn Ser Gln 
1               5                   10                  15      


Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val Ser Asp Cys Thr 
            20                  25                  30          


Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu Ser Glu Phe Leu 
        35                  40                  45              


Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His Lys Tyr Cys Asp 
    50                  55                  60                  


Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr Ser Glu Thr Asp 
65                  70                  75                  80  


Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr Ser Glu Ala Cys 
                85                  90                  95      


Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly Phe Gly Val Lys 
            100                 105                 110         


Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu Pro Cys Pro Val 
        115                 120                 125             


Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys Cys His Pro Trp 
    130                 135                 140                 


Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln Ala Gly Thr Asn 
145                 150                 155                 160 


Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu Arg Ala Leu Val 
                165                 170                 175     


Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu Leu Val Leu 
            180                 185                 190         


Val Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys 
        195                 200                 205             


Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn 
    210                 215                 220                 


Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr 
225                 230                 235                 240 


Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
                245                 250                 255 


<210>  32
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Extracellular CD40 protein

<400>  32

Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu Ile Asn Ser Gln 
1               5                   10                  15      


Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val Ser Asp Cys Thr 
            20                  25                  30          


Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu Ser Glu Phe Leu 
        35                  40                  45              


Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His Lys Tyr Cys Asp 
    50                  55                  60                  


Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr Ser Glu Thr Asp 
65                  70                  75                  80  


Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr Ser Glu Ala Cys 
                85                  90                  95      


Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly Phe Gly Val Lys 
            100                 105                 110         


Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu Pro Cys Pro Val 
        115                 120                 125             


Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys Cys His Pro Trp 
    130                 135                 140                 


Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln Ala Gly Thr Asn 
145                 150                 155                 160 


Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu Arg 
                165                 170             


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40 Transmembrane Domain

<400>  33

Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu 
1               5                   10                  15      


Leu Val Leu Val 
            20  


<210>  34
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40 Intracellular Domain

<400>  34

Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln 
1               5                   10                  15      


Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr 
            20                  25                  30          


Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln 
        35                  40                  45              


Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
    50                  55                  60          


<210>  35
<211>  63
<212>  PRT
<213>  Homo sapiens

<400>  35

Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln 
1               5                   10                  15      


Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr 
            20                  25                  30          


Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln 
        35                  40                  45              


Glu Asp Gly Lys Glu Ser Arg Ile Glu Ser Val Gln Glu Arg Gln 
    50                  55                  60              


<210>  36
<211>  74
<212>  PRT
<213>  Mus musculus

<400>  36

Lys Lys Val Val Lys Lys Pro Lys Asp Asn Glu Ile Leu Pro Pro Ala 
1               5                   10                  15      


Ala Arg Arg Gln Asp Pro Gln Glu Met Glu Asp Tyr Pro Gly His Asn 
            20                  25                  30          


Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr 
        35                  40                  45              


Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln Val 
    50                  55                  60                  


Thr Asp Ser Ile Ala Leu Arg Pro Leu Val 
65                  70                  


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG hinge

<400>  37

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  38
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG2 Transmembrane domain

<400>  38

Gly Leu Trp Thr Thr Ile Thr Ile Phe Ile Thr Leu Phe Leu Leu Ser 
1               5                   10                  15      


Val Cys Tyr Ser Ala Thr Ile Thr Phe Phe 
            20                  25      


<210>  39
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FkBP12v36

<400>  39

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  40
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FkBP12 domain

<400>  40

Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe 
1               5                   10                  15      


Pro Lys Arg Gly Gln Thr Cys Trp His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Pro Phe Lys Phe Met 
        35                  40                  45              


Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
    50                  55                  60                  


Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
65                  70                  75                  80  


Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
                85                  90                  95      


Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105     


<210>  41
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB segment of mTOR

<400>  41

Met Ala Ser Arg Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu 
1               5                   10                  15      


Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe 
            20                  25                  30          


Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr 
        35                  40                  45              


Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu 
    50                  55                  60                  


Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp 
65                  70                  75                  80  


Leu Thr Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
                85                  90                  95      


Lys Leu Glu Ser 
            100 


<210>  42
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry reporter

<400>  42

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  43
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eGFP reporter

<400>  43

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  44
<211>  220
<212>  PRT
<213>  Mus musculus

<400>  44

Met Pro Gly Gly Leu Glu Ala Leu Arg Ala Leu Pro Leu Leu Leu Phe 
1               5                   10                  15      


Leu Ser Tyr Ala Cys Leu Gly Pro Gly Cys Gln Ala Leu Arg Val Glu 
            20                  25                  30          


Gly Gly Pro Pro Ser Leu Thr Val Asn Leu Gly Glu Glu Ala Arg Leu 
        35                  40                  45              


Thr Cys Glu Asn Asn Gly Arg Asn Pro Asn Ile Thr Trp Trp Phe Ser 
    50                  55                  60                  


Leu Gln Ser Asn Ile Thr Trp Pro Pro Val Pro Leu Gly Pro Gly Gln 
65                  70                  75                  80  


Gly Thr Thr Gly Gln Leu Phe Phe Pro Glu Val Asn Lys Asn His Arg 
                85                  90                  95      


Gly Leu Tyr Trp Cys Gln Val Ile Glu Asn Asn Ile Leu Lys Arg Ser 
            100                 105                 110         


Cys Gly Thr Tyr Leu Arg Val Arg Asn Pro Val Pro Arg Pro Phe Leu 
        115                 120                 125             


Asp Met Gly Glu Gly Thr Lys Asn Arg Ile Ile Thr Ala Glu Gly Ile 
    130                 135                 140                 


Ile Leu Leu Phe Cys Ala Val Val Pro Gly Thr Leu Leu Leu Phe Arg 
145                 150                 155                 160 


Lys Arg Trp Gln Asn Glu Lys Phe Gly Val Asp Met Pro Asp Asp Tyr 
                165                 170                 175     


Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn Leu Asp Asp Cys Ser Met 
            180                 185                 190         


Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly Thr Tyr Gln Asp Val Gly 
        195                 200                 205             


Asn Leu His Ile Gly Asp Ala Gln Leu Glu Lys Pro 
    210                 215                 220 


<210>  45
<211>  228
<212>  PRT
<213>  Mus musculus

<400>  45

Met Ala Thr Leu Val Leu Ser Ser Met Pro Cys His Trp Leu Leu Phe 
1               5                   10                  15      


Leu Leu Leu Leu Phe Ser Gly Glu Pro Val Pro Ala Met Thr Ser Ser 
            20                  25                  30          


Asp Leu Pro Leu Asn Phe Gln Gly Ser Pro Cys Ser Gln Ile Trp Gln 
        35                  40                  45              


His Pro Arg Phe Ala Ala Lys Lys Arg Ser Ser Met Val Lys Phe His 
    50                  55                  60                  


Cys Tyr Thr Asn His Ser Gly Ala Leu Thr Trp Phe Arg Lys Arg Gly 
65                  70                  75                  80  


Ser Gln Gln Pro Gln Glu Leu Val Ser Glu Glu Gly Arg Ile Val Gln 
                85                  90                  95      


Thr Gln Asn Gly Ser Val Tyr Thr Leu Thr Ile Gln Asn Ile Gln Tyr 
            100                 105                 110         


Glu Asp Asn Gly Ile Tyr Phe Cys Lys Gln Lys Cys Asp Ser Ala Asn 
        115                 120                 125             


His Asn Val Thr Asp Ser Cys Gly Thr Glu Leu Leu Val Leu Gly Phe 
    130                 135                 140                 


Ser Thr Leu Asp Gln Leu Lys Arg Arg Asn Thr Leu Lys Asp Gly Ile 
145                 150                 155                 160 


Ile Leu Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro Ile 
                165                 170                 175     


Phe Leu Leu Leu Asp Lys Asp Asp Gly Lys Ala Gly Met Glu Glu Asp 
            180                 185                 190         


His Thr Tyr Glu Gly Leu Asn Ile Asp Gln Thr Ala Thr Tyr Glu Asp 
        195                 200                 205             


Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu His 
    210                 215                 220                 


Pro Gly Gln Glu 
225             


<210>  46
<211>  226
<212>  PRT
<213>  Homo sapiens

<400>  46

Met Pro Gly Gly Pro Gly Val Leu Gln Ala Leu Pro Ala Thr Ile Phe 
1               5                   10                  15      


Leu Leu Phe Leu Leu Ser Ala Val Tyr Leu Gly Pro Gly Cys Gln Ala 
            20                  25                  30          


Leu Trp Met His Lys Val Pro Ala Ser Leu Met Val Ser Leu Gly Glu 
        35                  40                  45              


Asp Ala His Phe Gln Cys Pro His Asn Ser Ser Asn Asn Ala Asn Val 
    50                  55                  60                  


Thr Trp Trp Arg Val Leu His Gly Asn Tyr Thr Trp Pro Pro Glu Phe 
65                  70                  75                  80  


Leu Gly Pro Gly Glu Asp Pro Asn Gly Thr Leu Ile Ile Gln Asn Val 
                85                  90                  95      


Asn Lys Ser His Gly Gly Ile Tyr Val Cys Arg Val Gln Glu Gly Asn 
            100                 105                 110         


Glu Ser Tyr Gln Gln Ser Cys Gly Thr Tyr Leu Arg Val Arg Gln Pro 
        115                 120                 125             


Pro Pro Arg Pro Phe Leu Asp Met Gly Glu Gly Thr Lys Asn Arg Ile 
    130                 135                 140                 


Ile Thr Ala Glu Gly Ile Ile Leu Leu Phe Cys Ala Val Val Pro Gly 
145                 150                 155                 160 


Thr Leu Leu Leu Phe Arg Lys Arg Trp Gln Asn Glu Lys Leu Gly Leu 
                165                 170                 175     


Asp Ala Gly Asp Glu Tyr Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn 
            180                 185                 190         


Leu Asp Asp Cys Ser Met Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly 
        195                 200                 205             


Thr Tyr Gln Asp Val Gly Ser Leu Asn Ile Gly Asp Val Gln Leu Glu 
    210                 215                 220                 


Lys Pro 
225     


<210>  47
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  47

Met Ala Arg Leu Ala Leu Ser Pro Val Pro Ser His Trp Met Val Ala 
1               5                   10                  15      


Leu Leu Leu Leu Leu Ser Ala Glu Pro Val Pro Ala Ala Arg Ser Glu 
            20                  25                  30          


Asp Arg Tyr Arg Asn Pro Lys Gly Ser Ala Cys Ser Arg Ile Trp Gln 
        35                  40                  45              


Ser Pro Arg Phe Ile Ala Arg Lys Arg Gly Phe Thr Val Lys Met His 
    50                  55                  60                  


Cys Tyr Met Asn Ser Ala Ser Gly Asn Val Ser Trp Leu Trp Lys Gln 
65                  70                  75                  80  


Glu Met Asp Glu Asn Pro Gln Gln Leu Lys Leu Glu Lys Gly Arg Met 
                85                  90                  95      


Glu Glu Ser Gln Asn Glu Ser Leu Ala Thr Leu Thr Ile Gln Gly Ile 
            100                 105                 110         


Arg Phe Glu Asp Asn Gly Ile Tyr Phe Cys Gln Gln Lys Cys Asn Asn 
        115                 120                 125             


Thr Ser Glu Val Tyr Gln Gly Cys Gly Thr Glu Leu Arg Val Met Gly 
    130                 135                 140                 


Phe Ser Thr Leu Ala Gln Leu Lys Gln Arg Asn Thr Leu Lys Asp Gly 
145                 150                 155                 160 


Ile Ile Met Ile Gln Thr Leu Leu Ile Ile Leu Phe Ile Ile Val Pro 
                165                 170                 175     


Ile Phe Leu Leu Leu Asp Lys Asp Asp Ser Lys Ala Gly Met Glu Glu 
            180                 185                 190         


Asp His Thr Tyr Glu Gly Leu Asp Ile Asp Gln Thr Ala Thr Tyr Glu 
        195                 200                 205             


Asp Ile Val Thr Leu Arg Thr Gly Glu Val Lys Trp Ser Val Gly Glu 
    210                 215                 220                 


His Pro Gly Gln Glu 
225                 


<210>  48
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  48

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  49
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  49

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  50
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  StrepTagII

<400>  50

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  51
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  51

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag

<400>  52

His His His His His His 
1               5       


<210>  53
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi tag

<400>  53

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  54
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sFLAG

<400>  54

Asp Tyr Lys Asp Glu 
1               5   


<210>  55
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tev

<400>  55

Glu Asn Leu Tyr Phe Gln Gly Gly Ser 
1               5                   


<210>  56
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  56

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  57
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Xpress tag

<400>  57

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


<210>  58
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin tag

<400>  58

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  59
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myc tag

<400>  59

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  60
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 1

<400>  60

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  61
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 3

<400>  61

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  62
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STREP tag

<400>  62

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  63
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 tag

<400>  63

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  64

<400>  64
000

<210>  65
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  (GlyGlyGlyGlySer) is repeated n times wherein n is an integerof 
       1, 2, 3, 4, 5, 6, 7, 8, 9 or 10

<400>  65

Gly Gly Gly Gly Ser 
1               5   


<210>  66
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  (GlyGlyGlySer) is repeated n times wherein n is an integerof 1, 
       2, 3, 4, 5, 6, 7, 8, 9 or 10

<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  (GlyGlyGlyGlySer) is repeated n times wherein n is an integerof 
       1, 2, 3, 4, 5, 6, 7, 8, 9 or 10

<400>  66

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  67
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  (GlyGlyGlySer) is repeated n times wherein n is an integerof 1, 
       2, 3, 4, 5, 6, 7, 8, 9 or 10

<220>
<221>  MISC_FEATURE
<222>  (5)..(7)
<223>  (GlyGlySer) is repeated n times wherein n is an integerof 1, 2, 
       3, 4, 5, 6, 7, 8, 9 or 10

<400>  67

Gly Gly Gly Ser Gly Gly Ser 
1               5           


<210>  68
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  (GlyGlyGlySer) is repeated n times wherein n is an integerof 1, 
       2, 3, 4, 5, 6, 7, 8, 9 or 10

<400>  68

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  69
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  69

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  70
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  70

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  71
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  71

Gly Gly Gly Gly Ser 
1               5   


<210>  72
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  72

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  73
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  73

Gly Gly Gly Ser 
1               


<210>  74
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  74

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  75
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  75

Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10      


<210>  76
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  76

Gly Gly Ser Gly Gly Gly Ser Gly Ser Gly 
1               5                   10  


<210>  77
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gly Ser linker

<400>  77

Gly Gly Ser Gly Gly Gly Ser Gly 
1               5               


<210>  78
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mouse palivizumab variable heavy chain

<400>  78

Gln Val Glu Leu Gln Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Ser Val Gly Trp Ile Arg Gln Pro Ser Gly Glu Gly Leu Glu 
        35                  40                  45              


Trp Leu Ala Asp Ile Trp Trp Asp Asp Lys Lys Asp Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val 
65                  70                  75                  80  


Phe Leu Lys Ile Thr Gly Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ser Met Ile Thr Asn Trp Tyr Phe Asp Val Trp Gly Ala 
            100                 105                 110         


Gly Thr Thr Val Thr Val Ser Ser 
        115                 120 


<210>  79
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mouse palivizumab variable light chain

<400>  79

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Gly Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Leu Ser Thr Ser Pro Lys Leu Gln Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Ile Gln Ala Glu 
65                  70                  75                  80  


Asp Val Ala Thr Tyr Tyr Cys Phe Arg Gly Ser Gly Tyr Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  80
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human palivizumab variable light chain

<400>  80

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Cys Gln Leu Ser Val Gly Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp 
65                  70                  75                  80  


Phe Ala Thr Tyr Tyr Cys Phe Gln Gly Ser Gly Tyr Pro Phe Thr Phe 
                85                  90                  95      


Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105     


<210>  81
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human palivizumab variable heavy chain

<400>  81

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Ser Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala Asp Ile Trp Trp Asp Asp Lys Lys Asp Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Lys Val Thr Asn Met Asp Pro Ala Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ser Met Ile Thr Asn Trp Tyr Phe Asp Val Trp Gly Ala 
            100                 105                 110         


Gly Thr Thr 
        115 


<210>  82
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRH1

<400>  82

Gly Ala Ser Ile Asn Ser Asp Asn Tyr Tyr Trp Thr 
1               5                   10          


<210>  83
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRH2

<400>  83

His Ile Ser Tyr Thr Gly Asn Thr Tyr Tyr Thr Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  84
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRH3

<400>  84

Cys Gly Ala Tyr Val Leu Ile Ser Asn Cys Gly Trp Phe Asp Ser 
1               5                   10                  15  


<210>  85
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRL1

<400>  85

Gln Ala Ser Gln Asp Ile Ser Thr Tyr Leu Asn 
1               5                   10      


<210>  86
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRL2

<400>  86

Gly Ala Ser Asn Leu Glu Thr 
1               5           


<210>  87
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-RSV antibody CDRL3

<400>  87

Gln Gln Tyr Gln Tyr Leu Pro Tyr Thr 
1               5                   


<210>  88
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRH1

<400>  88

Gly Phe Asp Phe Asp Asn Ala Trp 
1               5               


<210>  89
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRH2

<400>  89

Ile Thr Gly Pro Gly Glu Gly Trp Ser Val 
1               5                   10  


<210>  90
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRH3

<400>  90

Thr Gly Lys Tyr Tyr Asp Phe Trp Ser Gly Tyr Pro Pro Gly Glu Glu 
1               5                   10                  15      


Tyr Phe Gln Asp 
            20  


<210>  91
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRL1

<400>  91

Thr Gly Asp Ser Leu Arg Ser His Tyr Ala Ser 
1               5                   10      


<210>  92
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRL2

<400>  92

Gly Lys Asn Asn Arg Pro Ser 
1               5           


<210>  93
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10E8 anti-HIV antibody CDRL3

<400>  93

Ser Ser Arg Asp Lys Ser Gly Ser Arg Leu Ser Val 
1               5                   10          


<210>  94
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 antibody CDRH1

<400>  94

Gly Tyr Glu Phe Ile Asp Cys Thr 
1               5               


<210>  95
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 antibody CDRH2

<400>  95

Lys Pro Arg Gly Gly Ala Val Asn 
1               5               


<210>  96
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 antibody CDRH3

<400>  96

Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His Trp 
1               5                   10                  


<210>  97
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 antibody CDRL1

<400>  97

Gln Tyr Gly Ser 
1               


<210>  98
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 antibody CDRL3

<400>  98

Gln Gln Tyr Glu Phe 
1               5   


<210>  99
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRH1

<400>  99

Glu Val Gln Leu His Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Thr Val Ser Gly Phe Asn Ile Lys 
            20                  25                  30  


<210>  100
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRH2

<400>  100

Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
1               5                   10              


<210>  101
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRH3

<400>  101

Ala Thr Ile Lys Ala Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu 
1               5                   10                  15      


Ile Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Phe 
            20                  25                  30      


<210>  102
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRL1

<400>  102

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Val Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys 
            20              


<210>  103
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRL2

<400>  103

Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val Tyr 
1               5                   10                  15  


<210>  104
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (ab55) CDRL3

<400>  104

Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser 
1               5                   10                  15      


Leu Lys Ile Asn Ser Leu Gln Ser Glu Asp Phe Gly Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  105
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRH1

<400>  105

Tyr Thr Phe Thr Asp Tyr Ala Ile Thr 
1               5                   


<210>  106
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRH2

<400>  106

Gly Leu Ile Ser Thr Tyr Tyr Gly Asp Ser Phe Tyr Asn Gln Lys Phe 
1               5                   10                  15      


Lys Gly 
        


<210>  107
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRH3

<400>  107

Thr Ile Arg Asp Gly Lys Ala Met Asp Tyr 
1               5                   10  


<210>  108
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRL1

<400>  108

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  109
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRL2

<400>  109

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  110
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Dengue virus antibody (DB2-3) CDRL3

<400>  110

Ser Gln Ser Thr His Val Pro Tyr Thr 
1               5                   


<210>  111
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-pertussis antibody variable heavy chain

<400>  111

Gln Val Gln Leu Gln Gln Pro Gly Ser Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Lys Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Phe Pro Gly Ser Gly Ser Thr Asn Tyr Asp Glu Lys Phe 
    50                  55                  60                  


Asn Ser Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Trp Leu Ser Gly Ala Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser 
        115             


<210>  112
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-pertussis antibody variable light chain

<400>  112

Gln Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Phe Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Arg Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Leu Thr Ser Asn Leu Pro Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser His Pro Pro Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  113
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRH1

<400>  113

Ser Tyr Gly Met His Trp 
1               5       


<210>  114
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRH2

<400>  114

Val Ile Trp Leu Asp Gly Ser Asn Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly Arg 
        


<210>  115
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRH3

<400>  115

Ala Arg Asp Ile Phe Thr Val Ala Arg Gly Val Ile Ile Tyr Phe Asp 
1               5                   10                  15      


Tyr 
    


<210>  116
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRL1

<400>  116

Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala 
1               5                   10      


<210>  117
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRL2

<400>  117

Asp Ala Ser Asn Arg Ala Thr 
1               5           


<210>  118
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-hepatitis C antibody CDRL3

<400>  118

Gln Gln Arg Ser Asn Trp Val Thr 
1               5               


<210>  119
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-influenza virus antibody CDRH1

<400>  119

Gly Met Thr Ser Asn Ser Leu Ala 
1               5               


<210>  120
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-influenza virus antibody CDRH2

<400>  120

Ile Ile Pro Val Phe Glu Thr Pro 
1               5               


<210>  121
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-influenza virus antibody CDRH3

<400>  121

Ala Thr Ser Ala Gly Gly Ile Val Asn Tyr Tyr Leu Ser Phe Asn Ile 
1               5                   10                  15      


<210>  122
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-influenza virus antibody CDRL1

<400>  122

Gln Thr Ile Thr Thr Trp 
1               5       


<210>  123
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-influenza virus antibody CDRL3

<400>  123

Gln Gln Tyr Ser Thr Tyr Ser Gly Thr 
1               5                   


<210>  124
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRH1

<400>  124

Tyr Thr Phe Ile His Phe Gly Ile Ser Trp 
1               5                   10  


<210>  125
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRH2

<400>  125

Ile Asp Thr Asn Asn Gly Asn Thr Asn Tyr Ala Gln Ser Leu Gln Gly 
1               5                   10                  15      


<210>  126
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRH3

<400>  126

Arg Ala Leu Glu Met Gly His Arg Ser Gly Phe Pro Phe Asp Tyr 
1               5                   10                  15  


<210>  127
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRL1

<400>  127

Gly Gly His Asn Ile Gly Ala Lys Asn Val His 
1               5                   10      


<210>  128
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRL2

<400>  128

Tyr Asp Ser Asp Arg Pro Ser 
1               5           


<210>  129
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-EBV antibody AMM01 CDRL3

<400>  129

Cys Gln Val Trp Asp Ser Gly Arg Gly His Pro Leu Tyr Val 
1               5                   10                  


<210>  130
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  porcine teschovirus-1 P2A


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  (GlySerGly) residues can be added to the 5' end of the peptide to
       improve cleavage efficiency

<400>  130

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  131
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thosea asigna virus T2A


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  (GlySerGly) residues can be added to the 5' end of the peptide to
       improve cleavage efficiency

<400>  131

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  132
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  equine rhinitis A virus E2A


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  (GlySerGly) residues can be added to the 5' end of the peptide to
       improve cleavage efficiency

<400>  132

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro Pro 
            20                  


<210>  133
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  foot-and-mouth disease virus F2A


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  (GlySerGly) residues can be added to the 5' end of the peptide to
       improve cleavage efficiency

<400>  133

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  134
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  134
ucgaguugga uguggaaggu                                                   20


<210>  135
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  135
uuuucauccc cacuucacac                                                   20


<210>  136
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  136
ccucggggga gagagaggug                                                   20


<210>  137
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  137
ugggcucagg ugcuuccuca                                                   20


<210>  138
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  138
ucaaaguaga gcacauagga                                                   20


<210>  139
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  139
ccaucaaaag uccuuuuugg                                                   20


<210>  140
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  140
gugucuacac uuagccuuuc                                                   20


<210>  141
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  141
gggugaaauu ucccaacuuu                                                   20


<210>  142
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  142
ccggccuuuu uaccuugggg                                                   20


<210>  143
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for CIITA deletion

<400>  143
ucugcagccu ucccagagga                                                   20


<210>  144
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  144
uugcuccagg ccacagcacu                                                   20


<210>  145
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  145
ucgaccagcu ugacaucaca                                                   20


<210>  146
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  146
agaaucaaaa ucggugaaua                                                   20


<210>  147
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  147
caugugcaaa cgccuucaac                                                   20


<210>  148
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  148
aaaguuuagg uucguaucug                                                   20


<210>  149
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  149
uuugagaauc aaaaucggug                                                   20


<210>  150
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  150
auucucaaac aaauguguca                                                   20


<210>  151
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  151
cuuuuagaaa guuccuguga                                                   20


<210>  152
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  152
aaagcuuuuc ucgaccagcu                                                   20


<210>  153
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRAC deletion

<400>  153
gagucucuca gcugguacac                                                   20


<210>  154
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  154
cagaggaccu gaaaaacgug                                                   20


<210>  155
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  155
agguccucug gaaagggaag                                                   20


<210>  156
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  156
agccaucaga agcagagauc                                                   20


<210>  157
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  157
ggugugggag aucucugcuu                                                   20


<210>  158
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  158
gcccuauccu ggguccacuc                                                   20


<210>  159
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  159
uuccccuguu uucuuucaga                                                   20


<210>  160
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  160
uuucagacug uggcuucacc                                                   20


<210>  161
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  161
aggccucggc gcugacgauc                                                   20


<210>  162
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  162
caggccccac ucaccugcuc                                                   20


<210>  163
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for TRBC deletion

<400>  163
aggccccacu caccugcucu                                                   20


<210>  164
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  164
uggccuggag gcuauccagc                                                   20


<210>  165
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  165
ccgauauucc ucagguacuc                                                   20


<210>  166
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  166
gaguaccuga ggaauaucgg                                                   20


<210>  167
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  167
cucacgucau ccagcagaga                                                   20


<210>  168
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  168
cauucucugc uggaugacgu                                                   20


<210>  169
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  169
acuuuccauu cucugcugga                                                   20


<210>  170
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  170
cugaauugcu augugucugg                                                   20


<210>  171
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  171
auccauccga cauugaaguu                                                   20


<210>  172
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  172
aauucucucu ccauucuuca                                                   20


<210>  173
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for B2M deletion

<400>  173
agcaaggacu ggucuuucua                                                   20


<210>  174
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  174
aaaauguuuu aauguauaug uguua                                             25


<210>  175
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  175
aauguuuuaa uguauaugug uuau                                              24


<210>  176
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  176
uauuauaauu ucuuaaaauc ugcug                                             25


<210>  177
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  177
uauaauuucu uaaaaucugc ugugg                                             25


<210>  178
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  178
ucccugaaag aaaaucagug uuucu                                             25


<210>  179
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  179
cccugaaaga aaaucagugu uucuu                                             25


<210>  180
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  180
cccugacaga agcuacccua uguag                                             25


<210>  181
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  181
agcuacccua uguagaggac aaagu                                             25


<210>  182
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  182
aaguaggucu ucaauaaaua uuagu                                             25


<210>  183
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecule for RFX5 deletion

<400>  183
auuaguuggu uuacugcuuu uccca                                             25


<210>  184
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  184
cgcgggcgua cacacgcuga cgcgc                                             25


<210>  185
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  185
cguacacacg cugacgcgca ggcug                                             25


<210>  186
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  186
gcugacgcgc aggcugcggu cgcgc                                             25


<210>  187
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  187
caggcugcgg ucgcgcaggc gcagu                                             25


<210>  188
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  188
ggcugcgguc gcgcaggcgc agucg                                             25


<210>  189
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  189
cgcaggcgca gucggggcgc cuucc                                             25


<210>  190
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  190
cgcagucggg gcgccuuccc gguau                                             25


<210>  191
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  191
uuuaccccag cguguccuga gucuu                                             25


<210>  192
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA molecules for RFXAP deletion

<400>  192
agucuuuggu ucgcgaagug ccguu                                             25


<210>  193
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  193
cggctcctcc atagcgaccg                                                   20


<210>  194
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  194
cctccatagc gaccgcggcc                                                   20


<210>  195
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  195
gcgaccgcgg ccgagcgaac                                                   20


<210>  196
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  196
cgaccgcggc cgagcgaacc                                                   20


<210>  197
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  197
cggccgagcg aaccgggaaa                                                   20


<210>  198
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  198
agcgaaccgg gaaacggcac                                                   20


<210>  199
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  199
gaaccgggaa acggcaccgg                                                   20


<210>  200
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  200
gggaaacggc accggaagcc                                                   20


<210>  201
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  201
gaaacggcac cggaagcccg                                                   20


<210>  202
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequences for RFXANK deletion

<400>  202
aaacggcacc ggaagcccgg                                                   20


<210>  203
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40

<400>  203

Val Phe Ile Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His 
1               5                   10                  15      


Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly 
            20                  25                  30          


Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro 
        35                  40                  45              


Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg 
    50                  55                  60                  


Gln 
65  


