                         SEQUENCE LISTING

<110>  EmendoBio Inc.
 
<120>  OMNI-103 CRISPR NUCLEASE

<130>  91677-A-PCT/GJG/AWG

<150>  63/286,855
<151>  2021-12-09

<150>  63/214,506
<151>  2021-06-24

<150>  63/147,166
<151>  2021-02-08

<160>  135   

<170>  PatentIn version 3.5

<210>  1
<211>  1348
<212>  PRT
<213>  Unknown

<220>
<223>  OMNI-103

<400>  1

Met Ser Ile Lys Ser Asp Tyr Phe Leu Gly Leu Asp Ile Gly Thr Asp 
1               5                   10                  15      


Ser Ile Gly Trp Ala Val Thr Asp Pro Glu Tyr His Ile Leu Arg Arg 
            20                  25                  30          


Lys Gly Lys Ala Leu Trp Gly Ile Arg Leu Phe Asp Ala Ala Asn Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Thr Phe Arg Thr Ser Arg Arg Arg Ile Gln Arg 
    50                  55                  60                  


Arg Arg Gln Arg Ile Arg Leu Leu Gln Glu Leu Phe Ala Glu Glu Met 
65                  70                  75                  80  


Val Lys Leu Asp Pro Gly Phe Phe Gln Arg Leu Ser Asp Ser Ala Phe 
                85                  90                  95      


Trp Gln Glu Asp Lys Gln Glu Gln Gln Ile Tyr Ser Leu Phe Thr Cys 
            100                 105                 110         


Glu Asn Tyr Thr Asp Val Asp Tyr Tyr Arg Glu Tyr Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Ser Ala Leu Ile Gln Glu Lys Lys Glu Phe Asp Leu Arg 
    130                 135                 140                 


Leu Leu Tyr Leu Ala Leu His His Leu Met Lys His Arg Gly His Phe 
145                 150                 155                 160 


Leu Phe Asn Gly Ser Ile Asn Asn Val Thr Ser Phe His Thr Thr Phe 
                165                 170                 175     


Gln Thr Phe Ala Asp Cys Leu Tyr Asp Glu Phe Asp Ile Glu Leu Glu 
            180                 185                 190         


Cys Asp Ser Glu Asp Arg Phe Ala Glu Ile Leu Lys Asp Lys His Ala 
        195                 200                 205             


Arg Lys Thr Gly Lys Cys Ser Glu Leu Glu Ile Ile Cys His Ile Glu 
    210                 215                 220                 


Lys Ser Asn Lys Gln Leu Lys Glu Leu Phe Lys Leu Ile Thr Gly Met 
225                 230                 235                 240 


Lys Ala Ser Leu Ser Val Val Phe Gly Asp Asp Glu Leu Ala Glu Ile 
                245                 250                 255     


Glu His Asn Lys Ile Ser Phe Ser Glu Ser Ser Tyr Asp Glu Val Arg 
            260                 265                 270         


Leu Ala Leu Glu Asp Glu Ile Gln Glu Arg Thr Gly Ile Leu Asp Ile 
        275                 280                 285             


Phe His Ala Val Tyr Ser Trp Ala Ile Leu Ala Asp Ile Leu Glu Gly 
    290                 295                 300                 


Gly Glu Tyr Glu Gly Asn Ser Tyr Leu Ser Val Ala Lys Val Ser Thr 
305                 310                 315                 320 


Tyr Lys Lys His Gly Asp Asp Leu Arg Leu Leu Arg Thr Leu Val Arg 
                325                 330                 335     


Glu Tyr Cys Pro Asp His Tyr Lys Ser Phe Phe Ser Val Ser Gly Lys 
            340                 345                 350         


Glu Asn Tyr Cys Ala Tyr Ala Gly Thr Leu Lys Lys Asn Gly Lys Lys 
        355                 360                 365             


Gln Pro Ile Lys Arg Cys Ser Gln Glu Asp Phe Tyr Lys Ala Leu Lys 
    370                 375                 380                 


Lys Leu Leu Asn Gln Met Pro Thr Glu Gln Pro Glu Val Lys Asp Ile 
385                 390                 395                 400 


Phe Ile Glu Ile Glu Asn Gly Thr Phe Leu Pro Leu Gln Val Ser Lys 
                405                 410                 415     


Asp Asn Gly Val Ile Pro Tyr Gln Val Asn Lys Met Glu Leu Glu Lys 
            420                 425                 430         


Ile Leu Gln Asn Ala Glu Glu Tyr Leu Pro Phe Leu Lys Asn Ile Asp 
        435                 440                 445             


Glu Glu Cys Gly Lys Thr Val Ser Lys Lys Ile Ile Asp Leu Phe Glu 
    450                 455                 460                 


Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Thr Ala Lys Gly Glu 
465                 470                 475                 480 


Asn Cys Trp Met Val Arg Lys Glu Ala Gly Arg Ile Tyr Pro Trp Asn 
                485                 490                 495     


Phe Asp Glu Lys Val Asp Arg Asp Gln Ser Ala Glu Lys Phe Ile Arg 
            500                 505                 510         


Arg Met Thr Asn Gln Cys Thr Tyr Leu Ile His Glu Asp Val Val Pro 
        515                 520                 525             


Lys Asn Ser Leu Leu Tyr Ser Glu Phe Met Val Leu Asn Glu Leu Asn 
    530                 535                 540                 


Asn Val Lys Ile Arg Ser Glu Lys Leu Pro Val Glu Leu Lys Gln Ala 
545                 550                 555                 560 


Ile Val Leu Asp Leu Phe Lys Lys Gln Lys Gln Ile Thr Gly Lys Lys 
                565                 570                 575     


Leu Leu Asn Tyr Leu Asn Ala Asn Gly Tyr Asp Val Lys Lys Glu Asp 
            580                 585                 590         


Leu Ser Gly Phe Asp Gly Asn Phe Lys Ser Ser Leu Ser Ser Tyr Leu 
        595                 600                 605             


Thr Leu Lys Lys Val Phe Gly Glu Glu Leu Asp Lys Tyr Ser Val Gln 
    610                 615                 620                 


Gln Met Ala Glu Asp Ile Ile Leu Trp Ile Thr Leu Tyr Gly Asp Asp 
625                 630                 635                 640 


Gln Lys Met Leu Arg Arg Val Ile Arg Lys His Tyr Glu Gln Gln Leu 
                645                 650                 655     


Ser Glu Glu Gln Ile Leu Ser Leu Ser Lys Leu Lys Phe Gln Gly Trp 
            660                 665                 670         


Gly Arg Leu Ser Arg Arg Leu Leu Ser Glu Met Glu Gly Val Asp Cys 
        675                 680                 685             


Glu Thr Gly Glu Cys Met Thr Val Met Gln Gly Leu Arg Asn Thr Gln 
    690                 695                 700                 


Asn Asn Leu Met Gln Leu Leu Ser Gln Gln Phe Ser Phe Met Glu Leu 
705                 710                 715                 720 


Ile Glu Glu Glu Asn Gly Asn Tyr Tyr Val Asp Glu Ile Thr Tyr Asp 
                725                 730                 735     


Asn Leu Val Lys Asp Met Val Ile Ser Pro Ser Val Lys Arg Ala Val 
            740                 745                 750         


Trp Gln Thr Val Gln Ile Val Glu Glu Ile Lys Gly Val Met Gly Cys 
        755                 760                 765             


Gln Pro Lys Lys Ile Phe Val Glu Met Ala Arg Ser Asp Glu Glu Lys 
    770                 775                 780                 


Lys Arg Thr Val Ser Arg Lys Asp Arg Leu Leu Glu Ala Tyr Asp Ala 
785                 790                 795                 800 


Ile Lys Asp Glu Ala Arg Gln Trp Gln Glu Glu Leu Gln Lys Tyr Ser 
                805                 810                 815     


Asp Gly Asp Phe Lys Ala Ile Lys Leu Tyr Leu Tyr Tyr Thr Gln Met 
            820                 825                 830         


Gly Gln Cys Met Tyr Thr Gly Arg Lys Ile Asp Leu Ser Gln Leu Asn 
        835                 840                 845             


Asp Ala Thr Val Trp Asp Arg Asp His Ile Tyr Pro Gln Ser Lys Thr 
    850                 855                 860                 


Lys Asp Asp Ser Leu Asp Asn Leu Val Leu Val Asp Arg Ser Val Asn 
865                 870                 875                 880 


Ala Lys Lys Ser Asp Gly Met Leu Ser Pro Glu Ile Gln Gln Arg Met 
                885                 890                 895     


Arg Ala Thr Trp Lys Tyr Leu Lys Glu Lys Lys Leu Ile Ser Glu Lys 
            900                 905                 910         


Lys Tyr Glu Arg Leu Thr Arg Val Ser Pro Leu Thr Asp Glu Glu Leu 
        915                 920                 925             


Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ser Ser Lys 
    930                 935                 940                 


Ala Val Ala Thr Leu Leu Lys Arg Val Tyr Asp Glu Ala Glu Ile Val 
945                 950                 955                 960 


Tyr Val Lys Ala Glu Ala Val Ser Asn Phe Arg Arg Asp Asn Leu Asp 
                965                 970                 975     


Tyr Ile Lys Val Arg Asp Leu Asn Asp Tyr His His Ala Lys Asp Ala 
            980                 985                 990         


Tyr Gln Asn Ile Val Val Gly Asn  Val Phe His Glu Lys  Phe Thr Ser 
        995                 1000                 1005             


Asn Pro  Leu Arg Trp Leu Lys  Asn Asn Pro Asn Thr  Lys Tyr Ser 
    1010                 1015                 1020             


Leu Asn  Gln Met Phe Asn Phe  Asp Leu Glu Lys Asn  Gly Val Val 
    1025                 1030                 1035             


Ile Trp  Lys Arg Gly Lys Ala  Gly Ser Ile Lys Cys  Val Glu Glu 
    1040                 1045                 1050             


Thr Leu  Lys Arg Asn Asp Ile  Leu Phe Thr Arg Tyr  Ala Phe Cys 
    1055                 1060                 1065             


Asn Lys  Gly Gly Phe Phe Asn  Gln Met Leu Thr Ala  Ala Pro Glu 
    1070                 1075                 1080             


Asp Lys  Thr Lys Ala Lys Gly  Leu Val Pro Ile Lys  Lys Gly Met 
    1085                 1090                 1095             


Glu Thr  Trp Lys Tyr Gly Gly  Tyr Thr Ser Val Thr  Pro Ser His 
    1100                 1105                 1110             


Phe Met  Leu Val Ala Ser Lys  Asp Lys Lys Gly Lys  Glu Ile Arg 
    1115                 1120                 1125             


Thr Ile  Glu Thr Val Pro Leu  Tyr Arg Trp Lys Glu  Phe Lys Glu 
    1130                 1135                 1140             


Asn Pro  Asp Ala Leu Leu Gln  Tyr Cys Arg Glu Phe  Tyr Gly Leu 
    1145                 1150                 1155             


Lys Glu  Pro Lys Val Leu Ile  Pro Cys Ile Lys Lys  Asn Ala Arg 
    1160                 1165                 1170             


Leu Val  Val Asn Gly Phe Pro  Met His Leu Lys Gly  Ser Thr Gly 
    1175                 1180                 1185             


Lys Gln  Leu Ile Leu Gln Gly  Ala Val Gln Leu Cys  Leu Asn Asn 
    1190                 1195                 1200             


Glu Asn  Ile Lys Tyr Leu Lys  Lys Val Thr Lys Tyr  Leu Glu Tyr 
    1205                 1210                 1215             


Asn Ala  Gln Arg Arg Asp Lys  Arg Thr Leu Leu Glu  Val Arg Glu 
    1220                 1225                 1230             


Val Thr  Gly Ile Asn Lys Glu  Glu Asn Ile Gln Leu  Tyr Asp Val 
    1235                 1240                 1245             


Phe Val  Asp Lys Leu Ser Asn  Thr Ile Tyr Gln Tyr  Arg Pro Ala 
    1250                 1255                 1260             


Asn Pro  Lys Asp Asn Leu Ile  Lys Gly Arg Glu Lys  Phe Ile Glu 
    1265                 1270                 1275             


Leu Gly  Leu Ala Glu Gln Cys  Val Val Leu Gly Glu  Val Leu His 
    1280                 1285                 1290             


Leu Phe  Gln Cys Lys Pro Leu  Thr Ser Asp Leu Thr  Leu Ile Gly 
    1295                 1300                 1305             


Gly Ser  Pro Asn Thr Gly Thr  Ile Lys Ile Thr Lys  Thr Ile Ser 
    1310                 1315                 1320             


Asn Cys  Asn Val Val Lys Leu  Leu Ser Gln Ser Ile  Ala Gly Val 
    1325                 1330                 1335             


Lys Val  Arg Glu Ile Asn Leu  Leu Ile Ile 
    1340                 1345             


<210>  2
<211>  4047
<212>  DNA
<213>  Unknown

<220>
<223>  OMNI-103

<400>  2
atgagcataa aaagtgatta ttttttagga cttgatattg gtacggattc tattggatgg       60

gcggtaaccg acccagaata tcacatattg agacgaaaag gtaaagcatt atggggaata      120

agattatttg atgcggccaa tacagcggca gaacggcgaa catttaggac aagccgaaga      180

aggattcaga gaagacgaca gagaattcgg ttattgcaag aattatttgc agaagaaatg      240

gtaaaattag acccaggatt ttttcagagg ttgtcagaca gcgcattttg gcaggaggat      300

aagcaagagc agcaaattta ttcacttttt acttgtgaaa attatacaga tgttgattat      360

tacagagaat atcctactat ttatcatttg agaagtgcat tgattcagga aaagaaggaa      420

tttgatcttc gtcttctata tcttgctctt caccatttga tgaagcacag gggacatttc      480

ctgtttaatg ggagtattaa taatgtgacg tcatttcata cgacgtttca gacgtttgca      540

gattgtcttt atgatgagtt tgatatagaa ctggaatgtg attccgaaga tagatttgca      600

gaaattttaa aggataaaca tgccagaaaa acaggaaaat gttctgaatt agagataatc      660

tgtcatatag aaaaatcaaa taagcagcta aaagaacttt ttaaattaat tacaggaatg      720

aaagctagtt tgagtgttgt gtttggtgat gatgagttag cggaaataga acataataag      780

attagttttt cagagagtag ttatgatgaa gtacgtcttg cattggagga tgagattcag      840

gagaggactg gtatactgga tatctttcat gcagtttata gttgggcgat tctcgcggat      900

attttagaag gcggagaata tgaggggaat tcttatctaa gcgttgcgaa ggtaagcact      960

tataaaaagc atggtgatga tttgcggttg ctaagaacac tggttcggga atattgtcct     1020

gatcattaca aatctttctt ttccgtatca gggaaggaga attattgtgc atatgcgggt     1080

actttaaaaa agaatggaaa aaaacagccg attaaacgtt gcagccagga agatttttat     1140

aaagcgttaa agaaattgct gaatcagatg ccgacagaac aaccagaagt gaaagacatc     1200

ttcatcgaaa ttgaaaatgg tacttttttg ccgttgcagg taagtaagga taatggagtg     1260

ataccttatc aggtaaataa gatggaatta gaaaaaatcc tgcagaatgc agaggaatat     1320

ttgccatttc taaaaaatat agatgaagaa tgtggaaaaa cggttagtaa gaagattata     1380

gatctctttg agtttagaat accatattat gtagggccgc ttaataccgc taaaggagaa     1440

aactgttgga tggtcagaaa agaagcgggg agaatatatc cgtggaattt tgatgaaaag     1500

gtagacagag atcaatcagc agaaaaattt atccgtagaa tgacgaatca gtgtacatat     1560

ttaatacatg aggatgttgt acctaaaaat tctttgcttt attcggagtt tatggtgctt     1620

aatgaattaa ataatgtaaa gatccggtct gagaagctgc cggtggagtt aaaacaggca     1680

atagtattgg atttgtttaa gaaacaaaag cagataacag gaaaaaaact tcttaattac     1740

ttgaatgcaa atggatatga tgtaaaaaaa gaagatttgt cagggtttga cggaaacttt     1800

aaatcatctc tgtcatcata tcttactttg aaaaaagtat ttggtgaaga attagataaa     1860

tatagtgtgc agcagatggc agaggatatt atcttgtgga tcactctgta tggagatgat     1920

cagaagatgt tgcgcagggt aattcgaaaa cattatgaac agcaattgag tgaagaacag     1980

attctttcct tatcgaaatt gaaattccaa ggctggggaa gattatccag acgacttttg     2040

agtgaaatgg aaggcgttga ttgtgagact ggtgagtgta tgacggtcat gcaaggactt     2100

cgtaatactc agaataatct gatgcagctt ctaagtcagc agttttcatt tatggaattg     2160

attgaggaag aaaatgggaa ttattatgta gatgagatta catacgataa tcttgtgaaa     2220

gatatggtta tatctccgtc agtgaagaga gcagtctggc agacagttca gattgtggag     2280

gagattaagg gggtaatggg ctgtcagcct aagaagatat ttgtcgagat ggcgcgaagc     2340

gatgaagaga aaaagcgtac tgtatctagg aaagacaggt tattagaagc atatgatgcg     2400

atcaaggatg aggctcgtca atggcaggaa gagttgcaaa agtattcaga tggtgatttt     2460

aaggctatta aactttatct gtattatacg cagatggggc aatgtatgta tactggaaga     2520

aagatagatc tgtcacaatt aaatgatgcg acggtatggg acagagatca tatatatcca     2580

cagtccaaaa caaaagatga tagtctggat aatctggtat tggtagaccg gagcgtgaac     2640

gctaagaaaa gtgatgggat gctatcacct gagattcagc agagaatgcg ggctacttgg     2700

aaatacttaa aagagaaaaa gttgatttca gagaagaaat atgagcgttt gactagggtc     2760

tcaccactta cagatgagga attggcaggt tttattaatc gacagttagt tgaaacacgt     2820

cagtcttcga aagcagtagc aacacttttg aaacgagtat atgatgaagc ggagattgtc     2880

tatgtaaaag cggaagctgt ttcaaatttt agaagagata atttggatta tattaaggtg     2940

cgtgatctga atgattatca tcatgctaaa gatgcatatc agaatattgt agtggggaat     3000

gtttttcatg agaaatttac cagcaatccg cttcgttggc tgaaaaacaa tcctaatacg     3060

aaatatagtt taaatcagat gtttaacttt gatttagaga aaaatggggt ggtaatatgg     3120

aaaaggggga aggctggaag tattaaatgt gttgaagaaa cattgaaaag aaatgatatt     3180

ctttttacac gatatgcttt ttgtaataaa ggtggttttt ttaaccagat gttaacggca     3240

gctccagaag ataaaacgaa agcaaaggga cttgtaccaa taaaaaaagg tatggaaaca     3300

tggaaatacg ggggatatac atcagtaact ccgtcacatt ttatgttggt tgcttcgaaa     3360

gataagaaag gaaaggagat aagaacgatt gagacagttc cgttgtatag gtggaaagag     3420

ttcaaagaaa atccagatgc attactccaa tattgtagag agttctatgg tttgaaagag     3480

cccaaggtgt tgataccatg catcaagaag aatgccagat tagtcgttaa tggatttcca     3540

atgcatttga aagggagtac aggaaaacaa ttgattttgc agggagcggt tcaattatgt     3600

ctgaataatg aaaatataaa gtatttgaaa aaagtcacaa aatatttgga atataatgca     3660

cagagaagag ataaaagaac actgctggaa gtaagagagg ttacaggaat taacaaagag     3720

gaaaatatac agttatatga tgtgtttgtt gataaattga gtaacacaat atatcagtat     3780

cgtccggcca atccaaagga caatcttata aaaggaagag agaagtttat agaattaggg     3840

ttggcagaac aatgtgttgt gttaggtgaa gtattgcatt tgttccaatg taaaccactc     3900

acgtctgatt tgactttgat tggaggttca ccgaatacag ggacaataaa aataacaaag     3960

acaattagta attgtaatgt tgtaaagttg ttaagccaat ctattgcagg tgttaaagtg     4020

agagaaatta atttgttaat aatatga                                         4047


<210>  3
<211>  4047
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103 human optimized DNA sequence

<400>  3
atgtctatca agagcgacta cttcctgggc ctcgacatcg gcacagattc tatcggatgg       60

gccgtgacag accccgagta ccacatcctg agaagaaagg gcaaagccct gtggggcatc      120

agactgttcg atgccgccaa tactgccgcc gagagaagaa ccttccggac cagcagaaga      180

agaatccagc ggcggagaca gcggatcaga ctgctgcaag agctgttcgc cgaggaaatg      240

gttaagctgg accccggctt cttccagaga ctgagcgata gcgccttctg gcaagaggac      300

aagcaagagc agcagatcta cagcctgttt acctgcgaga actacaccga cgtggactac      360

tacagagagt accccaccat ctaccacctg agaagcgccc tgatccaaga gaaaaaagag      420

ttcgacctgc ggctgctgta tctggccctg caccatctga tgaagcaccg gggccacttt      480

ctgttcaacg gcagcatcaa caacgtgacc agcttccaca ccaccttcca gaccttcgcc      540

gactgcctgt acgacgagtt cgacatcgag ctggaatgcg acagcgagga cagattcgcc      600

gagatcctga aggataagca cgccagaaag accggcaagt gctctgagct ggaaatcatc      660

tgccacatcg agaagtccaa caagcagctg aaagaactgt tcaagctgat caccggcatg      720

aaggccagcc tgagcgtggt gtttggagat gatgagctgg ccgagatcga gcacaacaag      780

atcagcttca gcgagagcag ctacgacgaa gtgcggctgg ccctggaaga tgagattcaa      840

gagagaaccg gcatcctgga catcttccac gccgtgtatt cttgggccat cctggccgat      900

attctggaag gcggcgagta cgagggcaac agctatctgt ctgtggccaa ggtgtccacc      960

tacaagaagc acggcgacga cctgagactg ctgagaacac tcgtgcgcga gtactgcccc     1020

gaccactaca agagcttttt cagcgtgtcc ggcaaagaga actactgcgc ctacgccggc     1080

acactgaaga agaacggcaa gaagcagccc atcaagcggt gcagccaaga ggacttctac     1140

aaggccctga agaaactgct gaaccagatg cctaccgagc agcccgaagt gaaggatatc     1200

ttcatcgaga ttgagaacgg caccttcctg cctctgcaag tgtccaagga caacggcgtg     1260

atcccctacc aagtgaacaa gatggaactc gagaagatcc tgcagaacgc cgaagagtac     1320

ctgcctttcc tgaagaacat cgacgaggaa tgcggcaaga ccgtgtccaa gaagatcatc     1380

gacctgttcg agttcagaat cccctactac gtgggccctc tgaataccgc caagggcgag     1440

aattgctgga tggttcgaaa agaggccggc agaatctacc cctggaactt cgatgagaag     1500

gtggacagag atcagagcgc cgagaagttc atcagacgga tgaccaacca gtgcacctac     1560

ctgatccacg aggacgtggt gcctaagaac agcctgctgt actccgagtt catggtgctg     1620

aacgagctga acaatgtgaa gattcggagc gagaagctgc ccgtggaact gaagcaggcc     1680

atcgtgctgg acctgtttaa gaagcagaag cagatcacag ggaagaagct gctcaactac     1740

ctgaacgcca acggctacga cgtgaagaaa gaggacctga gcggcttcga cggcaacttc     1800

aagtccagcc tgtccagcta cctgactctg aagaaggtgt tcggagagga actggacaag     1860

tacagcgtgc agcagatggc cgaggacatc atcctgtgga tcaccctgta tggcgacgat     1920

cagaaaatgc tgcggagagt gatccggaag cactacgagc agcagctgtc tgaggaacag     1980

atcctgagcc tgagcaagct gaagttccaa ggctggggca gactgtctag acggctgctc     2040

tctgaaatgg aaggcgtgga ctgtgaaacc ggcgagtgca tgacagtgat gcagggcctg     2100

agaaacaccc agaacaacct gatgcagctg ctgagccagc agttcagctt catggaactg     2160

atcgaggaag agaacgggaa ctactacgtc gacgagatca cctacgacaa cctggtcaag     2220

gacatggtca tcagccctag cgtgaaaagg gccgtgtggc agacagtgca gatcgtggaa     2280

gaaatcaagg gcgtgatggg atgccagcct aagaaaatct tcgtggaaat ggcccgcagc     2340

gacgaagaga agaaacggac cgtgtctcgg aaggatcggc tgctggaagc ctacgacgcc     2400

atcaaggatg aggcccggca atggcaagaa gaactgcaga aatactccga cggcgatttc     2460

aaggccatca agctgtacct gtactacacc cagatgggcc agtgcatgta caccggcaga     2520

aaaatcgatc tgtcccagct gaacgacgcc accgtgtggg atagagatca catctaccct     2580

cagagcaaga ccaaggacga cagcctggac aatctggtgc tggtggatag atccgtgaat     2640

gccaagaaaa gcgacggcat gctgagcccc gagatccagc agagaatgag agccacctgg     2700

aagtacctga aagaaaagaa gctcatcagc gagaagaagt acgagcggct gaccagagtg     2760

tcccctctga cagatgaaga actggccggc ttcatcaacc ggcagctggt ggaaacaaga     2820

cagagcagca aagccgtggc cacactgctg aagagggtgt acgatgaggc cgagattgtg     2880

tatgtgaagg ccgaggccgt gtctaacttc cggcgggata acctggacta catcaaagtg     2940

cgggacctga acgactacca ccacgccaag gacgcctacc agaacatcgt cgtgggcaac     3000

gtgttccacg agaagtttac cagcaatccc ctgcggtggc tgaaaaacaa ccccaacacc     3060

aagtactccc tcaaccagat gttcaacttc gacctggaaa agaacggcgt ggtcatctgg     3120

aagagaggca aggccggctc cattaagtgt gtggaagaga cactgaagcg gaacgacatc     3180

ctgttcacca gatacgcttt ctgcaacaaa ggcggcttct ttaatcagat gctgaccgcc     3240

gctccagagg ataagacaaa ggccaaaggc ctggtgccta tcaagaaagg catggaaacc     3300

tggaaatacg gcggctacac cagcgtgacc cctagccact ttatgctggt ggccagcaag     3360

gacaagaagg gaaaagagat ccggaccatc gagacagtgc ccctgtaccg gtggaaagag     3420

ttcaaagaga atcccgacgc tctgctccag tactgcagag agttctacgg cctgaaagag     3480

cccaaggttc tgatcccttg catcaagaag aatgcccggc tggtcgtgaa cggcttccct     3540

atgcacctga agggcagcac cggaaaacag ctgattctgc agggtgccgt gcagctgtgc     3600

ctgaacaacg agaacatcaa gtacctcaag aaagtgacga agtacctcga gtacaacgcc     3660

cagcggagag acaagagaac cctgctcgaa gttcgggaag tgaccggaat caacaaagag     3720

gaaaacatcc agctgtacga tgtgttcgtg gacaagctga gcaacacaat ctaccagtac     3780

agacccgcca atcctaagga caacctcatc aagggccgcg agaaattcat cgagcttggc     3840

ctggctgagc agtgcgtggt gctgggagaa gtgctgcatc tgttccagtg caagcccctg     3900

accagcgatc tgacactgat cggcggaagc cctaacaccg gcaccatcaa gatcaccaag     3960

accatcagca actgcaacgt ggtcaagctg ctgtcccagt ctatcgccgg cgtgaaagtc     4020

cgcgagatca acctgctgat catctga                                         4047


<210>  4
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 crRNA repeat

<400>  4
guuugagagu aguguaa                                                      17


<210>  5
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 Partial crRNA 1

<400>  5
guuugagagu agugu                                                        15


<210>  6
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 Partial crRNA 2

<400>  6
guuugagagu ag                                                           12


<210>  7
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 Partial crRNA 3

<400>  7
guuugagagu                                                              10


<210>  8
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 tracrRNA antirepeat

<400>  8
uuacacuaca aguucaaau                                                    19


<210>  9
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 partial tracrRNA 1

<400>  9
acacuacaag uucaaau                                                      17


<210>  10
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 partial tracrRNA 2

<400>  10
cuacaaguuc aaau                                                         14


<210>  11
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 partial tracrRNA 3

<400>  11
acaaguucaa au                                                           12


<210>  12
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 tracrRNA portion 1

<400>  12
aaaaauuuau ucaaauccuu uugcuacauu guguagaauu u                           41


<210>  13
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 tracrRNA portion 2

<400>  13
aaagaucugg caacagaucu uuuuuu                                            26


<210>  14
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 tracrRNA portion 2 polyT

<400>  14
aaagaucugg caacagauc                                                    19


<210>  15
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 V1

<400>  15
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccuu       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  16
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 V2

<400>  16
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  17
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 12 V2 Modified tracrRNA

<400>  17
aaaaauuuau ucaaauccau uugcuacauu guguagaauu u                           41


<210>  18
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 crRNA repeat

<400>  18
guuugagagu aguguaa                                                      17


<210>  19
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial crRNA 1

<400>  19
guuugagagu agugu                                                        15


<210>  20
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial crRNA 2

<400>  20
guuugagagu ag                                                           12


<210>  21
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial crRNA 3

<400>  21
guuugagagu                                                              10


<210>  22
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 tracrRNA antirepeat

<400>  22
uuacacuaca aguucaaau                                                    19


<210>  23
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial tracrRNA 1

<400>  23
acacuacaag uucaaau                                                      17


<210>  24
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial tracrRNA 2

<400>  24
cuacaaguuc aaau                                                         14


<210>  25
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 Partial tracrRNA 3

<400>  25
acaaguucaa au                                                           12


<210>  26
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 tracrRNA portion 1

<400>  26
aaaaauuuau ucaaauccuu uugcuacauu guguagaauu u                           41


<210>  27
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 tracrRNA portion 2

<400>  27
aaagaucugg caacagaucu uuuuuauuuu uu                                     32


<210>  28
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 tracrRNA portion 2 polyT

<400>  28
aaagaucugg caacagaucu uuuuua                                            26


<210>  29
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 V1

<400>  29
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccuu       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuauuu uuu             113


<210>  30
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 V2

<400>  30
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccuu       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuu                     106


<210>  31
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 V3

<400>  31
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuu                     106


<210>  32
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 32 V3 modified tracrRNA

<400>  32
aaaaauuuau ucaaauccau uugcuacauu guguagaauu u                           41


<210>  33
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.1

<400>  33
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuaaaga ucuggcaaca gaucuuuuuu u                          101


<210>  34
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.2

<400>  34
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uuuuu                                             85


<210>  35
<211>  79
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.3

<400>  35
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuuuuu                                                    79


<210>  36
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.4

<400>  36
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuaaaga ugcaaaucuu uuuuu                                  95


<210>  37
<211>  6373
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET9a OMNI-103

<400>  37
taatacgact cactataggg agaccacaac ggtttccctc tagagagaca ataaccctga       60

taatgcttca ataatattga aaaaggaaga gtatgcctaa gaagaagaga aaggtgggta      120

cctctatcaa gagcgactac ttcctgggcc tcgacatcgg cacagattct atcggatggg      180

ccgtgacaga ccccgagtac cacatcctga gaagaaaggg caaagccctg tggggcatca      240

gactgttcga tgccgccaat actgccgccg agagaagaac cttccggacc agcagaagaa      300

gaatccagcg gcggagacag cggatcagac tgctgcaaga gctgttcgcc gaggaaatgg      360

ttaagctgga ccccggcttc ttccagagac tgagcgatag cgccttctgg caagaggaca      420

agcaagagca gcagatctac agcctgttta cctgcgagaa ctacaccgac gtggactact      480

acagagagta ccccaccatc taccacctga gaagcgccct gatccaagag aaaaaagagt      540

tcgacctgcg gctgctgtat ctggccctgc accatctgat gaagcaccgg ggccactttc      600

tgttcaacgg cagcatcaac aacgtgacca gcttccacac caccttccag accttcgccg      660

actgcctgta cgacgagttc gacatcgagc tggaatgcga cagcgaggac agattcgccg      720

agatcctgaa ggataagcac gccagaaaga ccggcaagtg ctctgagctg gaaatcatct      780

gccacatcga gaagtccaac aagcagctga aagaactgtt caagctgatc accggcatga      840

aggccagcct gagcgtggtg tttggagatg atgagctggc cgagatcgag cacaacaaga      900

tcagcttcag cgagagcagc tacgacgaag tgcggctggc cctggaagat gagattcaag      960

agagaaccgg catcctggac atcttccacg ccgtgtattc ttgggccatc ctggccgata     1020

ttctggaagg cggcgagtac gagggcaaca gctatctgtc tgtggccaag gtgtccacct     1080

acaagaagca cggcgacgac ctgagactgc tgagaacact cgtgcgcgag tactgccccg     1140

accactacaa gagctttttc agcgtgtccg gcaaagagaa ctactgcgcc tacgccggca     1200

cactgaagaa gaacggcaag aagcagccca tcaagcggtg cagccaagag gacttctaca     1260

aggccctgaa gaaactgctg aaccagatgc ctaccgagca gcccgaagtg aaggatatct     1320

tcatcgagat tgagaacggc accttcctgc ctctgcaagt gtccaaggac aacggcgtga     1380

tcccctacca agtgaacaag atggaactcg agaagatcct gcagaacgcc gaagagtacc     1440

tgcctttcct gaagaacatc gacgaggaat gcggcaagac cgtgtccaag aagatcatcg     1500

acctgttcga gttcagaatc ccctactacg tgggccctct gaataccgcc aagggcgaga     1560

attgctggat ggttcgaaaa gaggccggca gaatctaccc ctggaacttc gatgagaagg     1620

tggacagaga tcagagcgcc gagaagttca tcagacggat gaccaaccag tgcacctacc     1680

tgatccacga ggacgtggtg cctaagaaca gcctgctgta ctccgagttc atggtgctga     1740

acgagctgaa caatgtgaag attcggagcg agaagctgcc cgtggaactg aagcaggcca     1800

tcgtgctgga cctgtttaag aagcagaagc agatcacagg gaagaagctg ctcaactacc     1860

tgaacgccaa cggctacgac gtgaagaaag aggacctgag cggcttcgac ggcaacttca     1920

agtccagcct gtccagctac ctgactctga agaaggtgtt cggagaggaa ctggacaagt     1980

acagcgtgca gcagatggcc gaggacatca tcctgtggat caccctgtat ggcgacgatc     2040

agaaaatgct gcggagagtg atccggaagc actacgagca gcagctgtct gaggaacaga     2100

tcctgagcct gagcaagctg aagttccaag gctggggcag actgtctaga cggctgctct     2160

ctgaaatgga aggcgtggac tgtgaaaccg gcgagtgcat gacagtgatg cagggcctga     2220

gaaacaccca gaacaacctg atgcagctgc tgagccagca gttcagcttc atggaactga     2280

tcgaggaaga gaacgggaac tactacgtcg acgagatcac ctacgacaac ctggtcaagg     2340

acatggtcat cagccctagc gtgaaaaggg ccgtgtggca gacagtgcag atcgtggaag     2400

aaatcaaggg cgtgatggga tgccagccta agaaaatctt cgtggaaatg gcccgcagcg     2460

acgaagagaa gaaacggacc gtgtctcgga aggatcggct gctggaagcc tacgacgcca     2520

tcaaggatga ggcccggcaa tggcaagaag aactgcagaa atactccgac ggcgatttca     2580

aggccatcaa gctgtacctg tactacaccc agatgggcca gtgcatgtac accggcagaa     2640

aaatcgatct gtcccagctg aacgacgcca ccgtgtggga tagagatcac atctaccctc     2700

agagcaagac caaggacgac agcctggaca atctggtgct ggtggataga tccgtgaatg     2760

ccaagaaaag cgacggcatg ctgagccccg agatccagca gagaatgaga gccacctgga     2820

agtacctgaa agaaaagaag ctcatcagcg agaagaagta cgagcggctg accagagtgt     2880

cccctctgac agatgaagaa ctggccggct tcatcaaccg gcagctggtg gaaacaagac     2940

agagcagcaa agccgtggcc acactgctga agagggtgta cgatgaggcc gagattgtgt     3000

atgtgaaggc cgaggccgtg tctaacttcc ggcgggataa cctggactac atcaaagtgc     3060

gggacctgaa cgactaccac cacgccaagg acgcctacca gaacatcgtc gtgggcaacg     3120

tgttccacga gaagtttacc agcaatcccc tgcggtggct gaaaaacaac cccaacacca     3180

agtactccct caaccagatg ttcaacttcg acctggaaaa gaacggcgtg gtcatctgga     3240

agagaggcaa ggccggctcc attaagtgtg tggaagagac actgaagcgg aacgacatcc     3300

tgttcaccag atacgctttc tgcaacaaag gcggcttctt taatcagatg ctgaccgccg     3360

ctccagagga taagacaaag gccaaaggcc tggtgcctat caagaaaggc atggaaacct     3420

ggaaatacgg cggctacacc agcgtgaccc ctagccactt tatgctggtg gccagcaagg     3480

acaagaaggg aaaagagatc cggaccatcg agacagtgcc cctgtaccgg tggaaagagt     3540

tcaaagagaa tcccgacgct ctgctccagt actgcagaga gttctacggc ctgaaagagc     3600

ccaaggttct gatcccttgc atcaagaaga atgcccggct ggtcgtgaac ggcttcccta     3660

tgcacctgaa gggcagcacc ggaaaacagc tgattctgca gggtgccgtg cagctgtgcc     3720

tgaacaacga gaacatcaag tacctcaaga aagtgacgaa gtacctcgag tacaacgccc     3780

agcggagaga caagagaacc ctgctcgaag ttcgggaagt gaccggaatc aacaaagagg     3840

aaaacatcca gctgtacgat gtgttcgtgg acaagctgag caacacaatc taccagtaca     3900

gacccgccaa tcctaaggac aacctcatca agggccgcga gaaattcatc gagcttggcc     3960

tggctgagca gtgcgtggtg ctgggagaag tgctgcatct gttccagtgc aagcccctga     4020

ccagcgatct gacactgatc ggcggaagcc ctaacaccgg caccatcaag atcaccaaga     4080

ccatcagcaa ctgcaacgtg gtcaagctgc tgtcccagtc tatcgccggc gtgaaagtcc     4140

gcgagatcaa cctgctgatc atcggatcct acccatacga tgttccagat tacgcggccg     4200

ctccaaaaaa gaaaagaaaa gttgcggcta gccatcatca ccatcaccat catcattaag     4260

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     4320

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     4380

atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc     4440

aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac     4500

gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc     4560

gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc     4620

tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt     4680

catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt     4740

atcgatgata agctgtcaaa catgagaatt cttagaaaaa ctcatcgagc atcaaatgaa     4800

actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta     4860

atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg     4920

cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt     4980

tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat     5040

gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg     5100

catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc     5160

tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg     5220

catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc     5280

cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg     5340

tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat     5400

tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca     5460

atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata     5520

aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat     5580

ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc     5640

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     5700

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     5760

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     5820

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     5880

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     5940

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     6000

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     6060

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     6120

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     6180

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     6240

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     6300

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc     6360

gatcccgcga aat                                                        6373


<210>  38
<211>  2783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pShuttle Guide OMNI-103 V2

<400>  38
tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat       60

cgatgataag ctgtcaacac atttccccga aaagtgccac ctgacgtcct cgagtcccgc      120

ataatcgaaa tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg      180

ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata      240

cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa      300

tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct      360

tgctaatacg actcactata ggaagagcag agccttggtc tcgtttgaga gtagtgtaag      420

aaattacact acaagttcaa ataaaaattt attcaaatcc atttgctaca ttgtgtagaa      480

tttaaagatc tggcaacaga tctttttttg aattctctag cataacccct tggggcctct      540

aaacgggtct tgaggggttt tttgacctag gctaggggat atattccggg taccccgctt      600

cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg      660

aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc      720

cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg      780

ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg      840

cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt      900

atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca      960

agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact     1020

atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta     1080

attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca     1140

agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag     1200

agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg     1260

cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt     1320

tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg     1380

ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac     1440

aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagaag     1500

ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg     1560

tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc     1620

accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa     1680

cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat     1740

cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc     1800

tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag     1860

aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag     1920

cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg     1980

accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc     2040

gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg     2100

cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat     2160

gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg     2220

ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag     2280

caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac     2340

ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg     2400

atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt     2460

gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg tcgccatgat     2520

cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg     2580

gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag     2640

cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg     2700

cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac     2760

gatgagcgca ttgttagatt tca                                             2783


<210>  39
<211>  5009
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pbPOS T2 library


<220>
<221>  misc_feature
<222>  (3040)..(3047)
<223>  n is a, c, g, or t

<400>  39
tcgagtcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac       60

aatttcacac atgattacgg attcaacgtc gtgactggta aaacccgggc gttacccaac      120

ttaatcgcct tgcagcacat ccccctttcg ccagcaggcg taataaggaa aggattcatg      180

tactatttga aaaacacaaa cttttggatg ttcggtttat tctttttctt ttactttttt      240

atcatgggag cctacttccc gtttttcccg atttggctac atgatatcaa ccatatcagc      300

aaaagtgata cgggtattat ttttgccgct atttctctgt tctcgctatt attccaaccg      360

ctgtttggtc tgctttctga caaactcggt ctacgcaaat acctgctgtg gattattacc      420

ggcatgttag tgatgtttgc gccgttcttt atttttatct tcgggccact gctgcagtac      480

aacattttag tagggtcgat tgttggtggt atttatctag gctttagttt taacgccggt      540

gcgccagcag tagaggcatt tattgagaaa gtcagccggc gcagtaattt cgaatttggt      600

cgcgcgcgga tgtttggcag tgttggctgg gcgctggttg cctcgattgt cgggatcatg      660

ttcaccatta ataatcagtt tgttttctgg ctgggctctg gcagttgtct catcctcgcc      720

gttttactct ttttcgccaa aacggacgcg ccctcaagtg ccacggttgc caatgcggta      780

ggtgccaacc attcggcatt tagccttaag ctggcactgg aactgttcag acagccaaaa      840

ctgtggtttt tgtcactgta tgttattggc gtttcctcca cctacgatgt ttttgaccaa      900

cagtttgcta atttctttac ttcgttcttt gctaccggtg aacagggtac ccgcgtattt      960

ggctacgtaa cgacaatggg cgaattactt aacgcctcga ttatgttctt tgcgccactg     1020

atcattaatc gcatcggtgg gaagaatgcc ctgctgctgg ctggcactat tatgtctgta     1080

cgtattattg gctcatcgtt cgccacctca gcgctggaag tggttattct gaaaacgctg     1140

catatgtttg aagtaccgtt cctgctggtg ggctccttta aatatattac tagtcagttt     1200

gaagtgcgtt tttcagcgac gatttatctg gtcagtttca gcttctttaa gcaactggcg     1260

atgattttta tgtctgtact ggcgggcaat atgtatgaaa gcataggttt ccaaggcgct     1320

tatctggtgc tgggtctggt ggcgctgggc ttcaccttaa tttccgtgtt cacgcttagc     1380

ggcccgggcc cgctttccct gctgcgtcgt caggtgaatg aagtcgctta aaggcctcga     1440

tgcagctagc atgctaatct gattcgttac caattatgac aacttgacgg ctacatcatt     1500

cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa     1560

tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg     1620

catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct     1680

taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca     1740

aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta     1800

ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc     1860

catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc     1920

ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc     1980

ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg     2040

ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg     2100

acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac     2160

aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata     2220

acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg     2280

cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg     2340

cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg     2400

catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac     2460

cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa     2520

caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact     2580

ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc     2640

gcaactctct actgtttctc catacccgtt tttttggggt agcgattgaa aacgatgcag     2700

tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt     2760

gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg     2820

tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc     2880

atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat     2940

ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttttg gggaatataa     3000

tcttctagac atacaatgga agagcagagc cttggtctcn nnnnnnnaag cttgatatcg     3060

aattcctgca gcccggggga tcccatggta cgcgtgctag aggcatcaaa taaaacgaaa     3120

ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct     3180

gagtaggaca aatccgccgc cctagaccta ggcgttcggc tgcggcgagc ggtatcagct     3240

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     3300

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     3360

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     3420

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     3480

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     3540

gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     3600

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     3660

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     3720

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     3780

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     3840

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     3900

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc     3960

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg     4020

actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa     4080

atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga     4140

tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc     4200

tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca     4260

ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca     4320

tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa     4380

acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat     4440

cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg     4500

aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca     4560

ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa     4620

gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg     4680

ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct     4740

caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt     4800

tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta     4860

gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt     4920

ttcgccagat atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag     4980

gcgtatcacg aggccctttc gtcttcacc                                       5009


<210>  40
<211>  10286
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pmOMNI OMNI-103

<400>  40
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttgcca ccatgcctaa gaagaagaga aaggtgggta cctctatcaa      960

gagcgactac ttcctgggcc tcgacatcgg cacagattct atcggatggg ccgtgacaga     1020

ccccgagtac cacatcctga gaagaaaggg caaagccctg tggggcatca gactgttcga     1080

tgccgccaat actgccgccg agagaagaac cttccggacc agcagaagaa gaatccagcg     1140

gcggagacag cggatcagac tgctgcaaga gctgttcgcc gaggaaatgg ttaagctgga     1200

ccccggcttc ttccagagac tgagcgatag cgccttctgg caagaggaca agcaagagca     1260

gcagatctac agcctgttta cctgcgagaa ctacaccgac gtggactact acagagagta     1320

ccccaccatc taccacctga gaagcgccct gatccaagag aaaaaagagt tcgacctgcg     1380

gctgctgtat ctggccctgc accatctgat gaagcaccgg ggccactttc tgttcaacgg     1440

cagcatcaac aacgtgacca gcttccacac caccttccag accttcgccg actgcctgta     1500

cgacgagttc gacatcgagc tggaatgcga cagcgaggac agattcgccg agatcctgaa     1560

ggataagcac gccagaaaga ccggcaagtg ctctgagctg gaaatcatct gccacatcga     1620

gaagtccaac aagcagctga aagaactgtt caagctgatc accggcatga aggccagcct     1680

gagcgtggtg tttggagatg atgagctggc cgagatcgag cacaacaaga tcagcttcag     1740

cgagagcagc tacgacgaag tgcggctggc cctggaagat gagattcaag agagaaccgg     1800

catcctggac atcttccacg ccgtgtattc ttgggccatc ctggccgata ttctggaagg     1860

cggcgagtac gagggcaaca gctatctgtc tgtggccaag gtgtccacct acaagaagca     1920

cggcgacgac ctgagactgc tgagaacact cgtgcgcgag tactgccccg accactacaa     1980

gagctttttc agcgtgtccg gcaaagagaa ctactgcgcc tacgccggca cactgaagaa     2040

gaacggcaag aagcagccca tcaagcggtg cagccaagag gacttctaca aggccctgaa     2100

gaaactgctg aaccagatgc ctaccgagca gcccgaagtg aaggatatct tcatcgagat     2160

tgagaacggc accttcctgc ctctgcaagt gtccaaggac aacggcgtga tcccctacca     2220

agtgaacaag atggaactcg agaagatcct gcagaacgcc gaagagtacc tgcctttcct     2280

gaagaacatc gacgaggaat gcggcaagac cgtgtccaag aagatcatcg acctgttcga     2340

gttcagaatc ccctactacg tgggccctct gaataccgcc aagggcgaga attgctggat     2400

ggttcgaaaa gaggccggca gaatctaccc ctggaacttc gatgagaagg tggacagaga     2460

tcagagcgcc gagaagttca tcagacggat gaccaaccag tgcacctacc tgatccacga     2520

ggacgtggtg cctaagaaca gcctgctgta ctccgagttc atggtgctga acgagctgaa     2580

caatgtgaag attcggagcg agaagctgcc cgtggaactg aagcaggcca tcgtgctgga     2640

cctgtttaag aagcagaagc agatcacagg gaagaagctg ctcaactacc tgaacgccaa     2700

cggctacgac gtgaagaaag aggacctgag cggcttcgac ggcaacttca agtccagcct     2760

gtccagctac ctgactctga agaaggtgtt cggagaggaa ctggacaagt acagcgtgca     2820

gcagatggcc gaggacatca tcctgtggat caccctgtat ggcgacgatc agaaaatgct     2880

gcggagagtg atccggaagc actacgagca gcagctgtct gaggaacaga tcctgagcct     2940

gagcaagctg aagttccaag gctggggcag actgtctaga cggctgctct ctgaaatgga     3000

aggcgtggac tgtgaaaccg gcgagtgcat gacagtgatg cagggcctga gaaacaccca     3060

gaacaacctg atgcagctgc tgagccagca gttcagcttc atggaactga tcgaggaaga     3120

gaacgggaac tactacgtcg acgagatcac ctacgacaac ctggtcaagg acatggtcat     3180

cagccctagc gtgaaaaggg ccgtgtggca gacagtgcag atcgtggaag aaatcaaggg     3240

cgtgatggga tgccagccta agaaaatctt cgtggaaatg gcccgcagcg acgaagagaa     3300

gaaacggacc gtgtctcgga aggatcggct gctggaagcc tacgacgcca tcaaggatga     3360

ggcccggcaa tggcaagaag aactgcagaa atactccgac ggcgatttca aggccatcaa     3420

gctgtacctg tactacaccc agatgggcca gtgcatgtac accggcagaa aaatcgatct     3480

gtcccagctg aacgacgcca ccgtgtggga tagagatcac atctaccctc agagcaagac     3540

caaggacgac agcctggaca atctggtgct ggtggataga tccgtgaatg ccaagaaaag     3600

cgacggcatg ctgagccccg agatccagca gagaatgaga gccacctgga agtacctgaa     3660

agaaaagaag ctcatcagcg agaagaagta cgagcggctg accagagtgt cccctctgac     3720

agatgaagaa ctggccggct tcatcaaccg gcagctggtg gaaacaagac agagcagcaa     3780

agccgtggcc acactgctga agagggtgta cgatgaggcc gagattgtgt atgtgaaggc     3840

cgaggccgtg tctaacttcc ggcgggataa cctggactac atcaaagtgc gggacctgaa     3900

cgactaccac cacgccaagg acgcctacca gaacatcgtc gtgggcaacg tgttccacga     3960

gaagtttacc agcaatcccc tgcggtggct gaaaaacaac cccaacacca agtactccct     4020

caaccagatg ttcaacttcg acctggaaaa gaacggcgtg gtcatctgga agagaggcaa     4080

ggccggctcc attaagtgtg tggaagagac actgaagcgg aacgacatcc tgttcaccag     4140

atacgctttc tgcaacaaag gcggcttctt taatcagatg ctgaccgccg ctccagagga     4200

taagacaaag gccaaaggcc tggtgcctat caagaaaggc atggaaacct ggaaatacgg     4260

cggctacacc agcgtgaccc ctagccactt tatgctggtg gccagcaagg acaagaaggg     4320

aaaagagatc cggaccatcg agacagtgcc cctgtaccgg tggaaagagt tcaaagagaa     4380

tcccgacgct ctgctccagt actgcagaga gttctacggc ctgaaagagc ccaaggttct     4440

gatcccttgc atcaagaaga atgcccggct ggtcgtgaac ggcttcccta tgcacctgaa     4500

gggcagcacc ggaaaacagc tgattctgca gggtgccgtg cagctgtgcc tgaacaacga     4560

gaacatcaag tacctcaaga aagtgacgaa gtacctcgag tacaacgccc agcggagaga     4620

caagagaacc ctgctcgaag ttcgggaagt gaccggaatc aacaaagagg aaaacatcca     4680

gctgtacgat gtgttcgtgg acaagctgag caacacaatc taccagtaca gacccgccaa     4740

tcctaaggac aacctcatca agggccgcga gaaattcatc gagcttggcc tggctgagca     4800

gtgcgtggtg ctgggagaag tgctgcatct gttccagtgc aagcccctga ccagcgatct     4860

gacactgatc ggcggaagcc ctaacaccgg caccatcaag atcaccaaga ccatcagcaa     4920

ctgcaacgtg gtcaagctgc tgtcccagtc tatcgccggc gtgaaagtcc gcgagatcaa     4980

cctgctgatc atcggatcct acccatacga tgttccagat tacgcggccg ctccaaaaaa     5040

gaaaagaaaa gttgaattcg gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc     5100

cggcgacgtg gaggagaacc ccggccccat ggtgagcaag ggcgaggagg ataacatggc     5160

catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga     5220

gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct     5280

gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat     5340

gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc     5400

cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac     5460

cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg     5520

caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc     5580

ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa     5640

gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc     5700

cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga     5760

ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat     5820

ggacgagctg tacaagtagc tcgagtctag agggcccgtt taaacccgct gatcagcctc     5880

gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     5940

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     6000

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     6060

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga     6120

aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc     6180

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     6240

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     6300

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     6360

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     6420

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     6480

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     6540

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt     6600

cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat     6660

ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg     6720

caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg     6780

cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt     6840

tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt     6900

tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct     6960

gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt     7020

tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc     7080

tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag     7140

accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg     7200

gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac     7260

tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc     7320

gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc     7380

tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc     7440

ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg     7500

ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat     7560

gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc     7620

cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa     7680

gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat     7740

tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt     7800

tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg     7860

ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc     7920

agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata     7980

atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc     8040

attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga     8100

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc     8160

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct     8220

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     8280

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta     8340

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     8400

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     8460

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     8520

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     8580

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     8640

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     8700

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     8760

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     8820

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     8880

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     8940

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga     9000

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     9060

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     9120

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     9180

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     9240

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     9300

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     9360

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     9420

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     9480

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     9540

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     9600

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     9660

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     9720

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     9780

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     9840

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     9900

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     9960

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt    10020

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt    10080

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt    10140

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca    10200

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat    10260

ttccccgaaa agtgccacct gacgtc                                         10286


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA Tag amino acid sequence

<400>  41

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  42
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SV40 NLS amino acid sequence

<400>  42

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  43
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A amino acid sequence

<400>  43

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  44
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry amino acid sequence

<400>  44

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  45
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HA Tag DNA sequence

<400>  45
tacccatacg atgttccaga ttacgct                                           27


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 NLS DNA sequence

<400>  46
ccaaaaaaga aaagaaaagt t                                                 21


<210>  47
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2A DNA sequence

<400>  47
gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggcccc          57


<210>  48
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry DNA sequence

<400>  48
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag       60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc      120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc      180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac      240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc      300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac      360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta      420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc      480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct      540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc      600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa      660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta g               711


<210>  49
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_s11-ref

<400>  49
ggaccagagc gggaggguag ga                                                22


<210>  50
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_s12-ref

<400>  50
guaugccugc cgugugaacc au                                                22


<210>  51
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S26 -ref

<400>  51
ucucucucca uucuucagua ag                                                22


<210>  52
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S27 -ref

<400>  52
agaauugaaa aaguggagca uu                                                22


<210>  53
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S40 -ref

<400>  53
aagaauguaa gacuuacccc ac                                                22


<210>  54
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S41 -ref

<400>  54
ucagcagcuu acaaaagaau gu                                                22


<210>  55
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S48 -ref

<400>  55
cgucgcgcug gcgggcauuc cu                                                22


<210>  56
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S49 -ref

<400>  56
agacaucucg gcccgaaugc ug                                                22


<210>  57
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_CXCR4_S35-ref

<400>  57
cuggagugaa aacuugaaga cu                                                22


<210>  58
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_CXCR4_s93-ref

<400>  58
gggguucaga caacagugga ag                                                22


<210>  59
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_ELANE_g114-ref

<400>  59
gguguuaugg ucacagcggg ug                                                22


<210>  60
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_ELANE_g115-alt

<400>  60
ugggaauccc auucccgcag cu                                                22


<210>  61
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_ELANE_g128-ref

<400>  61
ugcuccccac ccgcucccag cc                                                22


<210>  62
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40-ref

<400>  62
aacacaucgg agagcuucgu gc                                                22


<210>  63
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S92-ref

<400>  63
gaggaccgca gccagcccgg cc                                                22


<210>  64
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SAMD9_g34-ref

<400>  64
gccaagaccc uuuaaacaga cc                                                22


<210>  65
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SAMD9_g36-ref

<400>  65
guaauaccag agugaagauu au                                                22


<210>  66
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SAMD9L_g133-alt

<400>  66
aggaacaaag agccuuuggu gc                                                22


<210>  67
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SAMD9L_g79-alt

<400>  67
ugacuucugu cuacgcuaca ga                                                22


<210>  68
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SAMD9L_g80-alt

<400>  68
gcauucuaga gccuggaauu ua                                                22


<210>  69
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SARM1_g42-ref

<400>  69
cgcgcggccu gcacacgcgu cu                                                22


<210>  70
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SARM1_g43-ref

<400>  70
cgccacugcg cgcuggcgcu gg                                                22


<210>  71
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SARM1_g44-ref

<400>  71
gugucugagc agcagcugcu gg                                                22


<210>  72
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_SARM1_g45-ref

<400>  72
gaugucuuca ucagcuaccg cc                                                22


<210>  73
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S124 -ref

<400>  73
ucucgaccag cuugacauca ca                                                22


<210>  74
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S141 -ref

<400>  74
cuugguuuua cagauacgaa cc                                                22


<210>  75
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S142 -ref

<400>  75
cgucaugagc agauuaaacc cg                                                22


<210>  76
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S24-ref

<400>  76
acugugcuag acaugagguc ua                                                22


<210>  77
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S35-ref

<400>  77
gacccugccg uguaccagcu ga                                                22


<210>  78
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S36-ref

<400>  78
ucaaaaucgg ugaauaggca ga                                                22


<210>  79
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S58-ref

<400>  79
agaacccuga cccugccgug ua                                                22


<210>  80
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_s90-ref

<400>  80
uucugaugug uauaucacag ac                                                22


<210>  81
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S91-ref

<400>  81
gcuguggccu ggagcaacaa au                                                22


<210>  82
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S12 spacer

<400>  82
guaugccugc cgugugaacc au                                                22


<210>  83
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S36 spacer

<400>  83
ucaaaaucgg ugaauaggca ga                                                22


<210>  84
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S35 spacer

<400>  84
gacccugccg uguaccagcu ga                                                22


<210>  85
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 25nt spacer

<400>  85
uccaacacau cggagagcuu cgugc                                             25


<210>  86
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 24nt spacer

<400>  86
ccaacacauc ggagagcuuc gugc                                              24


<210>  87
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 23nt spacer

<400>  87
caacacaucg gagagcuucg ugc                                               23


<210>  88
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 22nt spacer

<400>  88
aacacaucgg agagcuucgu gc                                                22


<210>  89
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 21nt spacer

<400>  89
acacaucgga gagcuucgug c                                                 21


<210>  90
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 20nt spacer

<400>  90
cacaucggag agcuucgugc                                                   20


<210>  91
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S12 with sgRNA 12 V2 scaffold

<400>  91
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  92
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S36 with sgRNA 12 V2 scaffold

<400>  92
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  93
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S35 with sgRNA 12 V2 scaffold

<400>  93
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  94
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 25nt with sgRNA 12 V2 scaffold

<400>  94
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  95
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 24nt with sgRNA 12 V2 scaffold

<400>  95
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  96
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 23nt with sgRNA 12 V2 scaffold

<400>  96
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  97
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 22nt with sgRNA 12 V2 scaffold

<400>  97
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  98
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 21nt with sgRNA 12 V2 scaffold

<400>  98
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  99
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 20nt with sgRNA 12 V2 scaffold

<400>  99
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaucug gcaacagauc uuuuuuu                    107


<210>  100
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_B2M_S12 full sgRNA

<400>  100
guaugccugc cgugugaacc auguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuaaagauc uggcaacaga      120

ucuuuuuuu                                                              129


<210>  101
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S36 full sgRNA

<400>  101
ucaaaaucgg ugaauaggca gaguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuaaagauc uggcaacaga      120

ucuuuuuuu                                                              129


<210>  102
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_TRAC_S35 full sgRNA

<400>  102
gacccugccg uguaccagcu gaguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuaaagauc uggcaacaga      120

ucuuuuuuu                                                              129


<210>  103
<211>  132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 25nt full sgRNA

<400>  103
uccaacacau cggagagcuu cgugcguuug agaguagugu aagaaauuac acuacaaguu       60

caaauaaaaa uuuauucaaa uccauuugcu acauugugua gaauuuaaag aucuggcaac      120

agaucuuuuu uu                                                          132


<210>  104
<211>  131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 24nt full sgRNA

<400>  104
ccaacacauc ggagagcuuc gugcguuuga gaguagugua agaaauuaca cuacaaguuc       60

aaauaaaaau uuauucaaau ccauuugcua cauuguguag aauuuaaaga ucuggcaaca      120

gaucuuuuuu u                                                           131


<210>  105
<211>  130
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 23nt full sgRNA

<400>  105
caacacaucg gagagcuucg ugcguuugag aguaguguaa gaaauuacac uacaaguuca       60

aauaaaaauu uauucaaauc cauuugcuac auuguguaga auuuaaagau cuggcaacag      120

aucuuuuuuu                                                             130


<210>  106
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 22nt full sgRNA

<400>  106
aacacaucgg agagcuucgu gcguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuaaagauc uggcaacaga      120

ucuuuuuuu                                                              129


<210>  107
<211>  128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 21nt full sgRNA

<400>  107
acacaucgga gagcuucgug cguuugagag uaguguaaga aauuacacua caaguucaaa       60

uaaaaauuua uucaaaucca uuugcuacau uguguagaau uuaaagaucu ggcaacagau      120

cuuuuuuu                                                               128


<210>  108
<211>  127
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103_PDCD1_S40 20nt full sgRNA

<400>  108
cacaucggag agcuucgugc guuugagagu aguguaagaa auuacacuac aaguucaaau       60

aaaaauuuau ucaaauccau uugcuacauu guguagaauu uaaagaucug gcaacagauc      120

uuuuuuu                                                                127


<210>  109
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.1 scaffold

<400>  109
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuaaaga ucuggcaaca gaucuuuuuu u                          101


<210>  110
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.2 scaffold

<400>  110
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uuuuu                                             85


<210>  111
<211>  79
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.3 scaffold

<400>  111
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuuuuu                                                    79


<210>  112
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.4 scaffold

<400>  112
guuugagagu aguggaaaca cuacaaguuc aaauaaaaau uuauucaaau ccauuugcua       60

cauuguguag aauuuaaaga ugcaaaucuu uuuuu                                  95


<210>  113
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  V2.5 scaffold

<400>  113
guuugagagu aguguaagaa auuacacuac aaguucaaau aaaaauuuau ucaaauccau       60

uugcuacauu guguagaauu uaaagaugca aaucuuuuuu u                          101


<210>  114
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA Repeat sequence A

<400>  114
guuugagagu agug                                                         14


<210>  115
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA Repeat sequence B

<400>  115
guuugagagu aguguaa                                                      17


<210>  116
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA antiepeat sequence A

<400>  116
cacuacaagu ucaaau                                                       16


<210>  117
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA antiepeat sequence B

<400>  117
uuacacuaca aguucaaau                                                    19


<210>  118
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 1 sequence A

<400>  118
aaaaauuuau ucaaauccau uugcuacauu guguagaauu u                           41


<210>  119
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 1 sequence B

<400>  119
aaaaauuuau ucaaauccau uugcuacauu guguagaauu uuuuu                       45


<210>  120
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 1  partial sequence

<400>  120
aaaaauuuau ucaaauccau uugcuacauu guguagaa                               38


<210>  121
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 2 sequence A

<400>  121
aaagaucugg caacagaucu uuuuuu                                            26


<210>  122
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 2 sequence B

<400>  122
aaagaugcaa aucuuuuuuu                                                   20


<210>  123
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 2  - partial sequence A

<400>  123
aaagaucugg caacaga                                                      17


<210>  124
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA Portion 2  - partial sequence B

<400>  124
aaagaugcaa auc                                                          13


<210>  125
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAC s91 spacer

<400>  125
gcuguggccu ggagcaacaa au                                                22


<210>  126
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PDCD1 s40 spacer

<400>  126
aacacaucgg agagcuucgu gc                                                22


<210>  127
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  B2M s12 spacer

<400>  127
guaugccugc cgugugaacc au                                                22


<210>  128
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TRAC s35 spacer

<400>  128
gacccugccg uguaccagcu ga                                                22


<210>  129
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103 v2.2 TRAC S35 sgRNA

<400>  129
gacccugccg uguaccagcu gaguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuuuuu                    107


<210>  130
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103 v2.3 TRAC S35 sgRNA

<400>  130
gacccugccg uguaccagcu gaguuugaga guaguggaaa cacuacaagu ucaaauaaaa       60

auuuauucaa auccauuugc uacauugugu agaauuuuuu u                          101


<210>  131
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103 v2.2 B2M S12 sgRNA

<400>  131
guaugccugc cgugugaacc auguuugaga guaguguaag aaauuacacu acaaguucaa       60

auaaaaauuu auucaaaucc auuugcuaca uuguguagaa uuuuuuu                    107


<210>  132
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OMNI-103 v2.3 B2M S12 sgRNA

<400>  132
guaugccugc cgugugaacc auguuugaga guaguggaaa cacuacaagu ucaaauaaaa       60

auuuauucaa auccauuugc uacauugugu agaauuuuuu u                          101


<210>  133
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1 S40 site


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, or t

<400>  133
gaccctgccg tgtaccagct gannract                                          28


<210>  134
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC S35 site


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, or t

<400>  134
aacacatcgg agagcttcgt gcnnract                                          28


<210>  135
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T2 sequence

<400>  135
ggaagagcag agccttggtc tc                                                22


