                         SEQUENCE LISTING

<110>  SilicoLife Lda.
 
<120>  Microbial Production of Tyrosol and Salidroside

<130>  sil102wo

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  635
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Ala Pro Val Thr Ile Glu Lys Phe Val Asn Gln Glu Glu Arg His 
1               5                   10                  15      


Leu Val Ser Asn Arg Ser Ala Thr Ile Pro Phe Gly Glu Tyr Ile Phe 
            20                  25                  30          


Lys Arg Leu Leu Ser Ile Asp Thr Lys Ser Val Phe Gly Val Pro Gly 
        35                  40                  45              


Asp Phe Asn Leu Ser Leu Leu Glu Tyr Leu Tyr Ser Pro Ser Val Glu 
    50                  55                  60                  


Ser Ala Gly Leu Arg Trp Val Gly Thr Cys Asn Glu Leu Asn Ala Ala 
65                  70                  75                  80  


Tyr Ala Ala Asp Gly Tyr Ser Arg Tyr Ser Asn Lys Ile Gly Cys Leu 
                85                  90                  95      


Ile Thr Thr Tyr Gly Val Gly Glu Leu Ser Ala Leu Asn Gly Ile Ala 
            100                 105                 110         


Gly Ser Phe Ala Glu Asn Val Lys Val Leu His Ile Val Gly Val Ala 
        115                 120                 125             


Lys Ser Ile Asp Ser Arg Ser Ser Asn Phe Ser Asp Arg Asn Leu His 
    130                 135                 140                 


His Leu Val Pro Gln Leu His Asp Ser Asn Phe Lys Gly Pro Asn His 
145                 150                 155                 160 


Lys Val Tyr His Asp Met Val Lys Asp Arg Val Ala Cys Ser Val Ala 
                165                 170                 175     


Tyr Leu Glu Asp Ile Glu Thr Ala Cys Asp Gln Val Asp Asn Val Ile 
            180                 185                 190         


Arg Asp Ile Tyr Lys Tyr Ser Lys Pro Gly Tyr Ile Phe Val Pro Ala 
        195                 200                 205             


Asp Phe Ala Asp Met Ser Val Thr Cys Asp Asn Leu Val Asn Val Pro 
    210                 215                 220                 


Arg Ile Ser Gln Gln Asp Cys Ile Val Tyr Pro Ser Glu Asn Gln Leu 
225                 230                 235                 240 


Ser Asp Ile Ile Asn Lys Ile Thr Ser Trp Ile Tyr Ser Ser Lys Thr 
                245                 250                 255     


Pro Ala Ile Leu Gly Asp Val Leu Thr Asp Arg Tyr Gly Val Ser Asn 
            260                 265                 270         


Phe Leu Asn Lys Leu Ile Cys Lys Thr Gly Ile Trp Asn Phe Ser Thr 
        275                 280                 285             


Val Met Gly Lys Ser Val Ile Asp Glu Ser Asn Pro Thr Tyr Met Gly 
    290                 295                 300                 


Gln Tyr Asn Gly Lys Glu Gly Leu Lys Gln Val Tyr Glu His Phe Glu 
305                 310                 315                 320 


Leu Cys Asp Leu Val Leu His Phe Gly Val Asp Ile Asn Glu Ile Asn 
                325                 330                 335     


Asn Gly His Tyr Thr Phe Thr Tyr Lys Pro Asn Ala Lys Ile Ile Gln 
            340                 345                 350         


Phe His Pro Asn Tyr Ile Arg Leu Val Asp Thr Arg Gln Gly Asn Glu 
        355                 360                 365             


Gln Met Phe Lys Gly Ile Asn Phe Ala Pro Ile Leu Lys Glu Leu Tyr 
    370                 375                 380                 


Lys Arg Ile Asp Val Ser Lys Leu Ser Leu Gln Tyr Asp Ser Asn Val 
385                 390                 395                 400 


Thr Gln Tyr Thr Asn Glu Thr Met Arg Leu Glu Asp Pro Thr Asn Gly 
                405                 410                 415     


Gln Ser Ser Ile Ile Thr Gln Val His Leu Gln Lys Thr Met Pro Lys 
            420                 425                 430         


Phe Leu Asn Pro Gly Asp Val Val Val Cys Glu Thr Gly Ser Phe Gln 
        435                 440                 445             


Phe Ser Val Arg Asp Phe Ala Phe Pro Ser Gln Leu Lys Tyr Ile Ser 
    450                 455                 460                 


Gln Gly Phe Phe Leu Ser Ile Gly Met Ala Leu Pro Ala Ala Leu Gly 
465                 470                 475                 480 


Val Gly Ile Ala Met Gln Asp His Ser Asn Ala His Ile Asn Gly Gly 
                485                 490                 495     


Asn Val Lys Glu Asp Tyr Lys Pro Arg Leu Ile Leu Phe Glu Gly Asp 
            500                 505                 510         


Gly Ala Ala Gln Met Thr Ile Gln Glu Leu Ser Thr Ile Leu Lys Cys 
        515                 520                 525             


Asn Ile Pro Leu Glu Val Ile Ile Trp Asn Asn Asn Gly Tyr Thr Ile 
    530                 535                 540                 


Glu Arg Ala Ile Met Gly Pro Thr Arg Ser Tyr Asn Asp Val Met Ser 
545                 550                 555                 560 


Trp Lys Trp Thr Lys Leu Phe Glu Ala Phe Gly Asp Phe Asp Gly Lys 
                565                 570                 575     


Tyr Thr Asn Ser Thr Leu Ile Gln Cys Pro Ser Lys Leu Ala Leu Lys 
            580                 585                 590         


Leu Glu Glu Leu Lys Asn Ser Asn Lys Arg Ser Gly Ile Glu Leu Leu 
        595                 600                 605             


Glu Val Lys Leu Gly Glu Leu Asp Phe Pro Glu Gln Leu Lys Cys Met 
    610                 615                 620                 


Val Glu Ala Ala Ala Leu Lys Arg Asn Lys Lys 
625                 630                 635 


<210>  2
<211>  356
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Gln Lys Asp Ala Leu Asn Asn Val His Ile Thr Asp Glu Gln Val 
1               5                   10                  15      


Leu Met Thr Pro Glu Gln Leu Lys Ala Ala Phe Pro Leu Ser Leu Gln 
            20                  25                  30          


Gln Glu Ala Gln Ile Ala Asp Ser Arg Lys Ser Ile Ser Asp Ile Ile 
        35                  40                  45              


Ala Gly Arg Asp Pro Arg Leu Leu Val Val Cys Gly Pro Cys Ser Ile 
    50                  55                  60                  


His Asp Pro Glu Thr Ala Leu Glu Tyr Ala Arg Arg Phe Lys Ala Leu 
65                  70                  75                  80  


Ala Ala Glu Val Ser Asp Ser Leu Tyr Leu Val Met Arg Val Tyr Phe 
                85                  90                  95      


Glu Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp Pro 
            100                 105                 110         


His Met Asp Gly Ser Phe Asp Val Glu Ala Gly Leu Gln Ile Ala Arg 
        115                 120                 125             


Lys Leu Leu Leu Glu Leu Val Asn Met Gly Leu Pro Leu Ala Thr Glu 
    130                 135                 140                 


Ala Leu Asn Pro Asn Ser Pro Gln Tyr Leu Gly Asp Leu Phe Ser Trp 
145                 150                 155                 160 


Ser Ala Ile Gly Ala Arg Thr Thr Glu Ser Gln Thr His Arg Glu Met 
                165                 170                 175     


Ala Ser Gly Leu Ser Met Pro Val Gly Phe Lys Asn Gly Thr Asp Gly 
            180                 185                 190         


Ser Leu Ala Thr Ala Ile Asn Ala Met Arg Ala Ala Ala Gln Pro His 
        195                 200                 205             


Arg Phe Val Gly Ile Asn Gln Ala Gly Gln Val Ala Leu Leu Gln Thr 
    210                 215                 220                 


Gln Gly Asn Pro Asp Gly His Val Ile Leu Arg Gly Gly Lys Ala Pro 
225                 230                 235                 240 


Asn Tyr Ser Pro Ala Asp Val Ala Gln Cys Glu Lys Glu Met Glu Gln 
                245                 250                 255     


Ala Gly Leu Arg Pro Ser Leu Met Val Asp Cys Ser His Gly Asn Ser 
            260                 265                 270         


Asn Lys Asp Tyr Arg Arg Gln Pro Ala Val Ala Glu Ser Val Val Ala 
        275                 280                 285             


Gln Ile Lys Asp Gly Asn Arg Ser Ile Ile Gly Leu Met Ile Glu Ser 
    290                 295                 300                 


Asn Ile His Glu Gly Asn Gln Ser Ser Glu Gln Pro Arg Ser Glu Met 
305                 310                 315                 320 


Lys Tyr Gly Val Ser Val Thr Asp Ala Cys Ile Ser Trp Glu Met Thr 
                325                 330                 335     


Asp Ala Leu Leu Arg Glu Ile His Gln Asp Leu Asn Gly Gln Leu Thr 
            340                 345                 350         


Ala Arg Val Ala 
        355     


<210>  3
<211>  373
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp 
1               5                   10                  15      


Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu 
            20                  25                  30          


Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu 
        35                  40                  45              


Arg Glu Ala Ser Ile Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu 
    50                  55                  60                  


Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg 
65                  70                  75                  80  


Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro 
                85                  90                  95      


Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg 
            100                 105                 110         


Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu 
        115                 120                 125             


Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly 
    130                 135                 140                 


Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly 
145                 150                 155                 160 


Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser 
                165                 170                 175     


Val Lys Asn Gly Pro Leu Gln Ala Met Leu Val Ala His Asp Gly Pro 
            180                 185                 190         


Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala 
        195                 200                 205             


Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln 
    210                 215                 220                 


Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile 
225                 230                 235                 240 


Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg 
                245                 250                 255     


His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val 
            260                 265                 270         


Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu 
        275                 280                 285             


Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala 
    290                 295                 300                 


Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr 
305                 310                 315                 320 


Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys 
                325                 330                 335     


Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp 
            340                 345                 350         


Tyr Val Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala 
        355                 360                 365             


Asn Asp Asn Arg Gln 
    370             


<210>  4
<211>  489
<212>  PRT
<213>  Arabidopsis thaliana

<400>  4

Met Gly Ser Gln Ile Ile His Asn Ser Gln Lys Pro His Val Val Cys 
1               5                   10                  15      


Val Pro Tyr Pro Ala Gln Gly His Ile Asn Pro Met Met Arg Val Ala 
            20                  25                  30          


Lys Leu Leu His Ala Arg Gly Phe Tyr Val Thr Phe Val Asn Thr Val 
        35                  40                  45              


Tyr Asn His Asn Arg Phe Leu Arg Ser Arg Gly Ser Asn Ala Leu Asp 
    50                  55                  60                  


Gly Leu Pro Ser Phe Arg Phe Glu Ser Ile Ala Asp Gly Leu Pro Glu 
65                  70                  75                  80  


Thr Asp Met Asp Ala Thr Gln Asp Ile Thr Ala Leu Cys Glu Ser Thr 
                85                  90                  95      


Met Lys Asn Cys Leu Ala Pro Phe Arg Glu Leu Leu Gln Arg Ile Asn 
            100                 105                 110         


Ala Gly Asp Asn Val Pro Pro Val Ser Cys Ile Val Ser Asp Gly Cys 
        115                 120                 125             


Met Ser Phe Thr Leu Asp Val Ala Glu Glu Leu Gly Val Pro Glu Val 
    130                 135                 140                 


Leu Phe Trp Thr Thr Ser Gly Cys Ala Phe Leu Ala Tyr Leu His Phe 
145                 150                 155                 160 


Tyr Leu Phe Ile Glu Lys Gly Leu Cys Pro Leu Lys Asp Glu Ser Tyr 
                165                 170                 175     


Leu Thr Lys Glu Tyr Leu Glu Asp Thr Val Ile Asp Phe Ile Pro Thr 
            180                 185                 190         


Met Lys Asn Val Lys Leu Lys Asp Ile Pro Ser Phe Ile Arg Thr Thr 
        195                 200                 205             


Asn Pro Asp Asp Val Met Ile Ser Phe Ala Leu Arg Glu Thr Glu Arg 
    210                 215                 220                 


Ala Lys Arg Ala Ser Ala Ile Ile Leu Asn Thr Phe Asp Asp Leu Glu 
225                 230                 235                 240 


His Asp Val Val His Ala Met Gln Ser Ile Leu Pro Pro Val Tyr Ser 
                245                 250                 255     


Val Gly Pro Leu His Leu Leu Ala Asn Arg Glu Ile Glu Glu Gly Ser 
            260                 265                 270         


Glu Ile Gly Met Met Ser Ser Asn Leu Trp Lys Glu Glu Met Glu Cys 
        275                 280                 285             


Leu Asp Trp Leu Asp Thr Lys Thr Gln Asn Ser Val Ile Tyr Ile Asn 
    290                 295                 300                 


Phe Gly Ser Ile Thr Val Leu Ser Val Lys Gln Leu Val Glu Phe Ala 
305                 310                 315                 320 


Trp Gly Leu Ala Gly Ser Gly Lys Glu Phe Leu Trp Val Ile Arg Pro 
                325                 330                 335     


Asp Leu Val Ala Gly Glu Glu Ala Met Val Pro Pro Asp Phe Leu Met 
            340                 345                 350         


Glu Thr Lys Asp Arg Ser Met Leu Ala Ser Trp Cys Pro Gln Glu Lys 
        355                 360                 365             


Val Leu Ser His Pro Ala Ile Gly Gly Phe Leu Thr His Cys Gly Trp 
    370                 375                 380                 


Asn Ser Ile Leu Glu Ser Leu Ser Cys Gly Val Pro Met Val Cys Trp 
385                 390                 395                 400 


Pro Phe Phe Ala Asp Gln Gln Met Asn Cys Lys Phe Cys Cys Asp Glu 
                405                 410                 415     


Trp Asp Val Gly Ile Glu Ile Gly Gly Asp Val Lys Arg Glu Glu Val 
            420                 425                 430         


Glu Ala Val Val Arg Glu Leu Met Asp Gly Glu Lys Gly Lys Lys Met 
        435                 440                 445             


Arg Glu Lys Ala Val Glu Trp Gln Arg Leu Ala Glu Lys Ala Thr Glu 
    450                 455                 460                 


His Lys Leu Gly Ser Ser Val Met Asn Phe Glu Thr Val Val Ser Lys 
465                 470                 475                 480 


Phe Leu Leu Gly Gln Lys Ser Gln Asp 
                485                 


<210>  5
<211>  1071
<212>  DNA
<213>  Escherichia coli

<400>  5
atgcaaaaag acgcgctgaa taacgtacat attaccgacg aacaggtttt aatgactccg       60

gaacaactga aggccgcttt tccattgagc ctgcaacaag aagcccagat tgctgactcg      120

cgtaaaagca tttcagatat tatcgccggg cgcgatcctc gtctgctggt agtatgtggt      180

ccttgttcca ttcatgatcc ggaaactgct ctggaatatg ctcgtcgatt taaagccctt      240

gccgcagagg tcagcgatag cctctatctg gtaatgcgcg tctattttga aaaaccccgt      300

accactgtcg gctggaaagg gttaattaac gatccccata tggatggctc ttttgatgta      360

gaagccgggc tgcagatcgc gcgtaaattg ctgcttgagc tggtgaatat gggactgcca      420

ctggcgacgg aagcgttaga tccgaatagc ccgcaatacc tgggcgatct gtttagctgg      480

tcagcaattg gtgctcgtac aacggaatcg caaactcacc gtgaaatggc ctccgggctt      540

tccatgccgg ttggttttaa aaacggcacc gacggcagtc tggcaacagc aattaacgct      600

atgcgcgccg ccgcccagcc gcaccgtttt gttggcatta accaggcagg gcaggttgcg      660

ttgctacaaa ctcaggggaa tccggacggc catgtgatcc tgcgcggtgg taaagcgccg      720

aactatagcc ctgcggatgt tgcgcaatgt gaaaaagaga tggaacaggc gggactgcgc      780

ccgtctctga tggtagattg cagccacggt aattccaata aagattatcg ccgtcagcct      840

gcggtggcag aatccgtggt tgctcaaatc aaagatggca atcgctcaat tattggtctg      900

atgatcgaaa gtaatatcca cgagggcaat cagtcttccg agcaaccgcg cagtgaaatg      960

aaatacggtg tatccgtaac cgatgcctgc attagctggg aaatgaccga tgccttgctg     1020

cgtgaaattc atcaggatct gaacgggcag ctgacggctc gcgtggctta a              1071


<210>  6
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aroFfbr (D147N)

<400>  6
atgcaaaaag acgcgctgaa taacgtacat attaccgacg aacaggtttt aatgactccg       60

gaacaactga aggccgcttt tccattgagc ctgcaacaag aagcccagat tgctgactcg      120

cgtaaaagca tttcagatat tatcgccggg cgcgatcctc gtctgctggt agtatgtggt      180

ccttgttcca ttcatgatcc ggaaactgct ctggaatatg ctcgtcgatt taaagccctt      240

gccgcagagg tcagcgatag cctctatctg gtaatgcgcg tctattttga aaaaccccgt      300

accactgtcg gctggaaagg gttaattaac gatccccata tggatggctc ttttgatgta      360

gaagccgggc tgcagatcgc gcgtaaattg ctgcttgagc tggtgaatat gggactgcca      420

ctggcgacgg aagcgttaaa tccgaatagc ccgcaatacc tgggcgatct gtttagctgg      480

tcagcaattg gtgctcgtac aacggaatcg caaactcacc gtgaaatggc ctccgggctt      540

tccatgccgg ttggttttaa aaacggcacc gacggcagtc tggcaacagc aattaacgct      600

atgcgcgccg ccgcccagcc gcaccgtttt gttggcatta accaggcagg gcaggttgcg      660

ttgctacaaa ctcaggggaa tccggacggc catgtgatcc tgcgcggtgg taaagcgccg      720

aactatagcc ctgcggatgt tgcgcaatgt gaaaaagaga tggaacaggc gggactgcgc      780

ccgtctctga tggtagattg cagccacggt aattccaata aagattatcg ccgtcagcct      840

gcggtggcag aatccgtggt tgctcaaatc aaagatggca atcgctcaat tattggtctg      900

atgatcgaaa gtaatatcca cgagggcaat cagtcttccg agcaaccgcg cagtgaaatg      960

aaatacggtg tatccgtaac cgatgcctgc attagctggg aaatgaccga tgccttgctg     1020

cgtgaaattc atcaggatct gaacgggcag ctgacggctc gcgtggctta a              1071


<210>  7
<211>  1470
<212>  DNA
<213>  Arabidopsis thaliana

<400>  7
atgggatctc agatcattca taactcacaa aaaccacatg tagtttgtgt tccatatccg       60

gctcaaggcc acatcaaccc tatgatgaga gtggctaaac tcctccacgc cagaggcttc      120

tacgtcacct tcgtcaacac cgtctacaac cacaatcgtt tccttcgttc tcgtgggtcc      180

aatgccctag atggacttcc ttcgttccga tttgagtcca ttgctgacgg tctaccagag      240

acagacatgg atgccacgca ggacatcaca gctctttgcg agtccaccat gaagaactgt      300

ctcgctccgt tcagagagct tctccagcgg atcaacgctg gagataatgt tcctccggta      360

agctgtattg tatctgacgg ttgtatgagc tttactcttg atgttgcgga ggagcttgga      420

gtcccggagg ttcttttttg gacaaccagt ggctgtgcgt tcctggctta tctacacttt      480

tatctcttca tcgagaaggg cttatgtccg ctaaaagatg agagttactt gacgaaggag      540

tacttagaag acacggttat agattttata ccaaccatga agaatgtgaa actaaaggat      600

attcctagct tcatacgtac cactaatcct gatgatgtta tgattagttt cgccctccgc      660

gagaccgagc gagccaaacg tgcttctgct atcattctaa acacatttga tgaccttgag      720

catgatgttg ttcatgctat gcaatctatc ttacctccgg tttattcagt tggaccgctt      780

catctcttag caaaccggga gattgaagaa ggtagtgaga ttggaatgat gagttcgaat      840

ttatggaaag aggagatgga gtgtttggat tggcttgata ctaagactca aaatagtgtc      900

atttatatca actttgggag cataacggtt ttgagtgtga agcagcttgt ggagtttgct      960

tggggtttgg cgggaagtgg gaaagagttt ttatgggtga tccggccaga tttagtagcg     1020

ggagaggagg ctatggttcc gccggacttt ttaatggaga ctaaagaccg cagtatgcta     1080

gcgagttggt gtcctcaaga gaaagtactt tctcatcctg ctattggagg gtttttgacg     1140

cattgcgggt ggaactcgat attggaaagt ctttcgtgtg gagttccgat ggtgtgttgg     1200

ccattttttg ctgaccagca aatgaattgt aagttttgtt gtgacgagtg ggatgttggg     1260

attgagatag gtggagatgt gaagagagag gaagttgagg cggtggttag agagctcatg     1320

gatggagaga agggaaagaa aatgagagaa aaggcggtag agtggcagcg cttagccgag     1380

aaagcgacgg aacataaact tggttcttcc gttatgaatt ttgagacggt tgttagcaag     1440

tttcttttgg gacaaaaatc acaggattaa                                      1470


<210>  8
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimized AtUGT85A1

<400>  8
atgggatcac agatcataca caactcgcag aaaccccatg tggtctgtgt accgtacccc       60

gctcagggcc atattaatcc aatgatgcgt gtcgctaaat tacttcatgc cagagggttt      120

tatgtaacat tcgtcaatac agtgtataat cacaatagat ttcttagaag ccgcgggtcg      180

aatgcgttag acggcctgcc ctccttccgg tttgagtcaa tagccgacgg gttgcctgaa      240

acggatatgg acgccacaca ggacataacg gctctgtgtg agtcgactat gaagaattgt      300

ctggctccct tccgcgagtt gctgcaacgg ataaatgctg gggataacgt acctcctgtt      360

agctgtatag tatccgatgg gtgcatgtcc tttacccttg atgtagcaga ggaacttgga      420

gtgccggaag ttttgttctg gaccactagc gggtgtgcct ttttagcata cctgcacttt      480

tatttattta tagaaaaggg gttgtgtccc ttaaaagacg agtcctattt gacgaaggaa      540

taccttgagg acacggttat agatttcata ccgactatga aaaacgttaa gctgaaggat      600

atacctagct tcatacgcac aactaatccg gatgatgtta tgatctcttt tgccctgcgt      660

gagacagagc gcgctaagcg ggcgtctgcg attatattga acacatttga cgatctggaa      720

cacgatgttg tccacgcaat gcagtccatt cttcctccgg tatattcagt gggacccttg      780

caccttttag cgaaccggga aatcgaagaa ggatctgaaa taggtatgat gtcttccaac      840

ttatggaagg aagaaatgga gtgtcttgac tggttggata caaagacaca aaattccgta      900

atatacataa acttcggcag catcacggtg ttgagcgtaa aacagctggt cgaattcgct      960

tggggtttgg caggttccgg taaggagttc ttgtgggtca taagaccaga cttagtcgcg     1020

ggggaagaag caatggtacc ccccgacttc cttatggaga cgaaagaccg ttccatgttg     1080

gcctcttggt gccctcaaga gaaagtcttg tcacatcccg ctattggagg gttcctgaca     1140

cactgtggtt ggaattcaat tcttgagagc ttatcgtgtg gggtgccaat ggtgtgctgg     1200

ccgttctttg cagatcagca aatgaactgt aagttttgct gcgacgaatg ggatgtaggt     1260

atagagatcg gcggcgacgt taagcgcgag gaggtcgagg cagttgtaag agagctgatg     1320

gacggtgaga aaggcaaaaa aatgagagaa aaagcggtcg agtggcagcg gttggctgag     1380

aaagctacgg aacataaact tggcagtagc gttatgaact ttgaaactgt tgtatcgaaa     1440

tttttgctgg ggcagaaaag ccaggactaa                                      1470


<210>  9
<211>  1908
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  9
atggcacctg ttacaattga aaagttcgta aatcaagaag aacgacacct tgtttccaac       60

cgatcagcaa caattccgtt tggtgaatac atatttaaaa gattgttgtc catcgatacg      120

aaatcagttt tcggtgttcc tggtgacttc aacttatctc tattagaata tctctattca      180

cctagtgttg aatcagctgg cctaagatgg gtcggcacgt gtaatgaact gaacgccgct      240

tatgcggccg acggatattc ccgttactct aataagattg gctgtttaat aaccacgtat      300

ggcgttggtg aattaagcgc cttgaacggt atagccggtt cgttcgctga aaatgtcaaa      360

gttttgcaca ttgttggtgt ggccaagtcc atagattcgc gttcaagtaa ctttagtgat      420

cggaacctac atcatttggt cccacagcta catgattcaa attttaaagg gccaaatcat      480

aaagtatatc atgatatggt aaaagataga gtcgcttgct cggtagccta cttggaggat      540

attgaaactg catgtgacca agtcgataat gttatccgcg atatttacaa gtattctaaa      600

cctggttata tttttgttcc tgcagatttt gcggatatgt ctgttacatg tgataatttg      660

gttaatgttc cacgtatatc tcaacaagat tgtatagtat acccttctga aaaccaattg      720

tctgacataa tcaacaagat tactagttgg atatattcca gtaaaacacc tgcgatcctt      780

ggagacgtac tgactgatag gtatggtgtg agtaactttt tgaacaagct tatctgcaaa      840

actgggattt ggaatttttc cactgttatg ggaaaatctg taattgatga gtcaaaccca      900

acttatatgg gtcaatataa tggtaaagaa ggtttaaaac aagtctatga acattttgaa      960

ctgtgcgact tggtcttgca ttttggagtc gacatcaatg aaattaataa tgggcattat     1020

acttttactt ataaaccaaa tgctaaaatc attcaatttc atccgaatta tattcgcctt     1080

gtggacacta ggcagggcaa tgagcaaatg ttcaaaggaa tcaattttgc ccctatttta     1140

aaagaactat acaagcgcat tgacgtttct aaactttctt tgcaatatga ttcaaatgta     1200

actcaatata cgaacgaaac aatgcggtta gaagatccta ccaatggaca atcaagcatt     1260

attacacaag ttcacttaca aaagacgatg cctaaatttt tgaaccctgg tgatgttgtc     1320

gtttgtgaaa caggctcttt tcaattctct gttcgtgatt tcgcgtttcc ttcgcaatta     1380

aaatatatat cgcaaggatt tttcctttcc attggcatgg cccttcctgc cgccctaggt     1440

gttggaattg ccatgcaaga ccactcaaac gctcacatca atggtggcaa cgtaaaagag     1500

gactataagc caagattaat tttgtttgaa ggtgacggtg cagcacagat gacaatccaa     1560

gaactgagca ccattctgaa gtgcaatatt ccactagaag ttatcatttg gaacaataac     1620

ggctacacta ttgaaagagc catcatgggc cctaccaggt cgtataacga cgttatgtct     1680

tggaaatgga ccaaactatt tgaagcattc ggagacttcg acggaaagta tactaatagc     1740

actctcattc aatgtccctc taaattagca ctgaaattgg aggagcttaa gaattcaaac     1800

aaaagaagcg ggatagaact tttagaagtc aaattaggcg aattggattt ccccgaacag     1860

ctaaagtgca tggttgaagc agcggcactt aaaagaaata aaaaatag                  1908


<210>  10
<211>  1917
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimized ScARO10*

<400>  10
atggctccgg ttaccatcga aaaattcgtt aaccaggaag aacgtcacct ggtttctaac       60

cgttctgcta ccatcccgtt cggtgaatac atcttcaaac gtctgctgtc tatcgacacc      120

aaatctgttt tcggtgttcc gggtgacttc aacctgtctc tgctggaata cctgtactct      180

ccgtctgttg aatctgctgg tctgcgttgg gttggtacct gcaacgaact gaacgctgct      240

tacgctgctg acggttactc tcgttactct aacaaaatcg gttgcctgat caccacctac      300

ggtgttggtg aactgtctgc tctgaacggt atcgctggtt ctttcgctga aaacgttaaa      360

gttctgcaca tcgttggtgt tgctaaatct atcgactctc gttcttctaa cttctctgac      420

cgtaacctgc accacctggt tccgcagctg cacgactcta acttcaaagg tccgaaccac      480

aaagtttacc acgacatggt taaagaccgt gttgcttgct ctgttgctta cctggaagac      540

atcgaaaccg cttgcgacca ggttgacaac gttatccgtg acatctacaa atactctaaa      600

ccgggttaca tcttcgttcc ggctgacttc gctgacatgt ctgttacctg cgacaacctg      660

gttaacgttc cgcgtatctc tcagcaggac tgcatcgttt acccgtctga aaaccagctg      720

tctgacatca tcaacaaaat cacctcttgg atctactctt ctaaaacccc ggctatcctg      780

ggtgacgttt taaccgaccg ttacggtgta agcaacttcc tgaacaaact gatctgcaaa      840

accggtatct ggaacttctc taccgttatg ggtaaatctg ttatcgacga atctaacccg      900

acctacatgg gtcagtacaa cggtaaagaa ggtctgaaac aggtttacga acacttcgaa      960

ctgtgcgacc tggttctgca cttcggtgtt gacatcaacg aaatcaacaa cggtcactac     1020

accttcacct acaaaccgaa cgctaaaatc atccagttcc acccgaacta catccgtctg     1080

gttgacaccc gtcagggtaa cgaacagatg ttcaaaggta tcaacttcgc tccgatcctg     1140

aaagaactgt acaaacgtat cgacgtttct aaactgtctc tgcagtacga ctctaacgtt     1200

acccagtaca ccaacgaaac catgcgtctg gaagacccga ccaacggtca gtcttctatc     1260

atcacccagg ttcacctgca gaaaaccatg ccgaaattcc tgaacccggg tgacgttgtt     1320

gtttgcgaaa ccggttcttt ccagttctct gttcgtgact tcgctttccc gtctcagctg     1380

aaatacatct ctcagggttt cttcctgtct atcggtatgg ctctgccggc tgctctgggt     1440

gttggtatcg ctatgcagga ccactctaac gctcacatca acggtggtaa cgttaaagaa     1500

gactacaaac cgcgtctgat cctgttcgaa ggtgacggtg ctgctcagat gaccatccag     1560

gaactgtcta ccatcctgaa atgcaacatc ccgctggaag ttatcatctg gaacaacaac     1620

ggttacacca tcgaacgtgc tatcatgggt ccgacccgtt cttacaacga cgttatgtct     1680

tggaaatgga ccaaactgtt cgaagcgttc ggtgacttcg acggtaaata caccaactct     1740

accctgatcc agtgcccgtc taaactggct ctgaaactgg aagaactgaa aaactctaac     1800

aaacgttctg gtatcgaact gctggaagtt aaactgggtg aactggactt cccggaacag     1860

ctgaaatgca tggttgaagc tgctgctctg aaacgtaaca aaaaataaaa gctttaa        1917


<210>  11
<211>  1122
<212>  DNA
<213>  Escherichia coli

<400>  11
atggttgctg aattgaccgc attacgcgat caaattgatg aagtcgataa agcgctgctg       60

aatttattag cgaagcgtct ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt      120

ggactgccta tttatgttcc ggagcgcgag gcatctatgt tggcctcgcg tcgtgcagag      180

gcggaagctc tgggtgtacc gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt      240

gaatcttact ccagtgaaaa cgacaaagga tttaaaacac tttgtccgtc actgcgtccg      300

gtggttatcg tcggcggtgg cggtcagatg ggacgcctgt tcgagaagat gctgaccctc      360

tcgggttatc aggtgcggat tctggagcaa catgactggg atcgagcggc tgatattgtt      420

gccgatgccg gaatggtgat tgttagtgtg ccaatccacg ttactgagca agttattggc      480

aaattaccgc ctttaccgaa agattgtatt ctggtcgatc tggcatcagt gaaaaatggg      540

ccattacagg ccatgctggt ggcgcatgat ggtccggtgc tggggctaca cccgatgttc      600

ggtccggaca gcggtagcct ggcaaagcaa gttgtggtct ggtgtgatgg acgtaaaccg      660

gaagcatacc aatggtttct ggagcaaatt caggtctggg gcgctcggct gcatcgtatt      720

agcgccgtcg agcacgatca gaatatggcg tttattcagg cactgcgcca ctttgctact      780

tttgcttacg ggctgcacct ggcagaagaa aatgttcagc ttgagcaact tctggcgctc      840

tcttcgccga tttaccgcct tgagctggcg atggtcgggc gactgtttgc tcaggatccg      900

cagctttatg ccgacatcat tatgtcgtca gagcgtaatc tggcgttaat caaacgttac      960

tataagcgtt tcggcgaggc gattgagttg ctggagcagg gcgataagca ggcgtttatt     1020

gacagtttcc gcaaggtgga gcactggttc ggcgattacg cacagcgttt tcagagtgaa     1080

agccgcgtgt tattgcgtca ggcgaatgac aatcgccagt aa                        1122


<210>  12
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tyrA^fbr (M53I and A354V)

<400>  12
atggttgctg aattgaccgc attacgcgat caaattgatg aagtcgataa agcgctgctg       60

aatttattag cgaagcgtct ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt      120

ggactgccta tttatgttcc ggagcgcgag gcatctatct tggcctcgcg tcgtgcagag      180

gcggaagctc tgggtgtacc gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt      240

gaatcttact ccagtgaaaa cgacaaagga tttaaaacac tttgtccgtc actgcgtccg      300

gtggttatcg tcggcggtgg cggtcagatg ggacgcctgt tcgagaagat gctgaccctc      360

tcgggttatc aggtgcggat tctggagcaa catgactggg atcgagcggc tgatattgtt      420

gccgatgccg gaatggtgat tgttagtgtg ccaatccacg ttactgagca agttattggc      480

aaattaccgc ctttaccgaa agattgtatt ctggtcgatc tggcatcagt gaaaaatggg      540

ccattacagg ccatgctggt ggcgcatgat ggtccggtgc tggggctaca cccgatgttc      600

ggtccggaca gcggtagcct ggcaaagcaa gttgtggtct ggtgtgatgg acgtaaaccg      660

gaagcatacc aatggtttct ggagcaaatt caggtctggg gcgctcggct gcatcgtatt      720

agcgccgtcg agcacgatca gaatatggcg tttattcagg cactgcgcca ctttgctact      780

tttgcttacg ggctgcacct ggcagaagaa aatgttcagc ttgagcaact tctggcgctc      840

tcttcgccga tttaccgcct tgagctggcg atggtcgggc gactgtttgc tcaggatccg      900

cagctttatg ccgacatcat tatgtcgtca gagcgtaatc tggcgttaat caaacgttac      960

tataagcgtt tcggcgaggc gattgagttg ctggagcagg gcgataagca ggcgtttatt     1020

gacagtttcc gcaaggtgga gcactggttc ggcgattacg tacagcgttt tcagagtgaa     1080

agccgcgtgt tattgcgtca ggcgaatgac aatcgccagt aa                        1122


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
ctcgagcacc accaccac                                                     18


<210>  14
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
actttaagaa ggagatatac atatggctcc ggttaccatc g                           41


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
ctcgagcacc accaccac                                                     18


<210>  16
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
aacaaaatta tttctattag gtaccttatt ttttgttacg tttcagagca g                51


<210>  17
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
cccaagcttt tattttttgt tacgtttcag agcag                                  35


<210>  18
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
gtttaacttt ataaggagga aaaaaaatgc aaaaagacgc gctga                       45


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
cggaagcgtt aaatccgaat ag                                                22


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
ctattcggat ttaacgcttc cg                                                22


<210>  21
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
aacaaaatta tttctattag gtaccttaag ccacgcgagc cgtc                        44


<210>  22
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
gtggcttaag cggcctaata cgactcacta taggggaatt                             40


<210>  23
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
tttctattag cggccgaatt cttagtgacg gaaatcaatc                             40


<210>  24
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
aacaaaatta tttctattag gtaccgggga attgttatcc gctc                        44


<210>  25
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
ggtacctaat agaaataatt ttgtttaact ttataaggag gaaaaaaa                    48


<210>  26
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
cagtggtggt ggtggtggtg ctcgagttac tggcgattgt cattcg                      46


<210>  27
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
cccccatggg atcacagatc atacac                                            26


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
ccggatcctt agtcctggct tttc                                              24


<210>  29
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
ttgacaatta atcatccggc tcgtataatg ggaattgtga gcggataaca attc             54


<210>  30
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
cattatacga gccggatgat taattgtcaa gcaggagtcg cataagggag agc              53


<210>  31
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  31
taatacgact cactatag                                                     18


<210>  32
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  32
tttacacttt atgcttccgg ctcgtatgtt g                                      31


<210>  33
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  33
ttgacaatta atcatcggct cgtataatg                                         29


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  34
ttgacaatta atcatccggc tcgtataatg                                        30


<210>  35
<211>  520
<212>  PRT
<213>  Escherichia coli

<400>  35

Met Lys Pro Glu Asp Phe Arg Ala Ser Thr Gln Arg Pro Phe Thr Gly 
1               5                   10                  15      


Glu Glu Tyr Leu Lys Ser Leu Gln Asp Gly Arg Glu Ile Tyr Ile Tyr 
            20                  25                  30          


Gly Glu Arg Val Lys Asp Val Thr Thr His Pro Ala Phe Arg Asn Ala 
        35                  40                  45              


Ala Ala Ser Val Ala Gln Leu Tyr Asp Ala Leu His Lys Pro Glu Met 
    50                  55                  60                  


Gln Asp Ser Leu Cys Trp Asn Thr Asp Thr Gly Ser Gly Gly Tyr Thr 
65                  70                  75                  80  


His Lys Phe Phe Arg Val Ala Lys Ser Ala Asp Asp Leu Arg Gln Gln 
                85                  90                  95      


Arg Asp Ala Ile Ala Glu Trp Ser Arg Leu Ser Tyr Gly Trp Met Gly 
            100                 105                 110         


Arg Thr Pro Asp Tyr Lys Ala Ala Phe Gly Cys Ala Leu Gly Ala Asn 
        115                 120                 125             


Pro Gly Phe Tyr Gly Gln Phe Glu Gln Asn Ala Arg Asn Trp Tyr Thr 
    130                 135                 140                 


Arg Ile Gln Glu Thr Gly Leu Tyr Phe Asn His Ala Ile Val Asn Pro 
145                 150                 155                 160 


Pro Ile Asp Arg His Leu Pro Thr Asp Lys Val Lys Asp Val Tyr Ile 
                165                 170                 175     


Lys Leu Glu Lys Glu Thr Asp Ala Gly Ile Ile Val Ser Gly Ala Lys 
            180                 185                 190         


Val Val Ala Thr Asn Ser Ala Leu Thr His Tyr Asn Met Ile Gly Phe 
        195                 200                 205             


Gly Thr Leu Glu Val Met Gly Glu Asn Pro Asp Phe Ala Leu Met Phe 
    210                 215                 220                 


Val Ala Pro Met Asp Ala Asp Gly Val Lys Leu Ile Ser Arg Ala Ser 
225                 230                 235                 240 


Tyr Glu Met Val Ala Gly Ala Thr Gly Ser Pro Tyr Asp Tyr Pro Leu 
                245                 250                 255     


Ser Ser Arg Phe Asp Glu Asn Asp Ala Ile Leu Val Met Asp Asn Val 
            260                 265                 270         


Leu Ile Pro Trp Glu Asn Val Leu Ile Tyr Arg Asp Phe Asp Arg Cys 
        275                 280                 285             


Arg Arg Trp Thr Met Glu Gly Gly Phe Ala Arg Met Tyr Pro Leu Gln 
    290                 295                 300                 


Ala Cys Val Arg Leu Ala Val Lys Leu Asp Phe Ile Thr Ala Leu Leu 
305                 310                 315                 320 


Lys Lys Ser Leu Glu Cys Thr Gly Thr Leu Glu Phe Arg Gly Val Gln 
                325                 330                 335     


Ala Asp Leu Gly Glu Val Val Ala Trp Arg Asn Thr Phe Trp Ala Leu 
            340                 345                 350         


Ser Asp Ser Met Cys Ser Glu Ala Thr Pro Trp Val Asn Gly Ala Tyr 
        355                 360                 365             


Leu Pro Asp His Ala Ala Leu Gln Thr Tyr Arg Val Leu Ala Pro Met 
    370                 375                 380                 


Ala Tyr Ala Lys Ile Lys Asn Ile Ile Glu Arg Asn Val Thr Ser Gly 
385                 390                 395                 400 


Leu Ile Tyr Leu Pro Ser Ser Ala Arg Asp Leu Asn Asn Pro Gln Ile 
                405                 410                 415     


Asp Gln Tyr Leu Ala Lys Tyr Val Arg Gly Ser Asn Gly Met Asp His 
            420                 425                 430         


Val Gln Arg Ile Lys Ile Leu Lys Leu Met Trp Asp Ala Ile Gly Ser 
        435                 440                 445             


Glu Phe Gly Gly Arg His Glu Leu Tyr Glu Ile Asn Tyr Ser Gly Ser 
    450                 455                 460                 


Gln Asp Glu Ile Arg Leu Gln Cys Leu Arg Gln Ala Gln Asn Ser Gly 
465                 470                 475                 480 


Asn Met Asp Lys Met Met Ala Met Val Asp Arg Cys Leu Ser Glu Tyr 
                485                 490                 495     


Asp Gln Asp Gly Trp Thr Val Pro His Leu His Asn Asn Asp Asp Ile 
            500                 505                 510         


Asn Met Leu Asp Lys Leu Leu Lys 
        515                 520 


<210>  36
<211>  1563
<212>  DNA
<213>  Escherichia coli

<400>  36
atgaaaccag aagatttccg cgccagtacc caacgtcctt tcaccgggga agagtatctg       60

aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcacc      120

actcatccgg catttcgtaa tgcggcagcg tctgttgccc agctgtacga cgcactgcac      180

aaaccggaga tgcaggactc tctgtgttgg aacaccgaca ccggcagcgg cggctatacc      240

cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccagcaacg cgacgccatc      300

gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagccgct      360

ttcggttgcg cactgggcgc gaatccgggc ttttacggtc agttcgagca gaacgcccgt      420

aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca      480

ccgatcgatc gtcatttgcc gaccgataaa gtgaaagacg tttacatcaa gctggaaaaa      540

gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa ctcggcgctg      600

actcactaca acatgattgg cttcggctcg gcacaagtga tgggcgaaaa cccggacttc      660

gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga aattaatctc ccgcgcctct      720

tatgagatgg tcgcgggtgc taccggctcg ccatacgact acccgctctc cagccgcttc      780

gatgagaacg atgcgattct ggtgatggat aacgtgctga ttccatggga aaacgtgctg      840

atctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt tgcccgtatg      900

tatccgctgc aagcctgtgt gcgcctggca gtgaaattag acttcattac ggcactgctg      960

aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc cgatctcggt     1020

gaagtggtag cgtggcgcaa caccttctgg gcattgagtg actcgatgtg ttcagaagca     1080

acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac ctatcgcgta     1140

ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt taccagtggc     1200

ctgatctatc tcccttccag tgcccgtgac ctgaataatc cgcagatcga ccagtatctg     1260

gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa gatcctcaaa     1320

ctgatgtggg atgctattgg cagcgaattt ggtggtcgtc acgaactgta tgaaatcaac     1380

tactccggta gccaggatga gattcgcctg cagtgtctgc gccaggcaca aaactccggc     1440

aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga ccaggacggc     1500

tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa gctgctgaaa     1560

taa                                                                   1563


<210>  37
<211>  1563
<212>  DNA
<213>  Escherichia coli

<400>  37
atgaaaccag aagatttccg cgccagtacc caacgtcctt tcaccgggga agagtatctg       60

aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcacc      120

actcatccgg catttcgtaa tgcggcagcg tctgttgccc agctgtacga cgcactgcac      180

aaaccggaga tgcaggactc tctgtgttgg aacaccgaca ccggcagcgg cggctatacc      240

cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccagcaacg cgacgccatc      300

gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagccgct      360

ttcggttgcg cactgggcgc gaatccgggc ttttacggtc agttcgagca gaacgcccgt      420

aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca      480

ccgatcgatc gtcatttgcc gaccgataaa gtgaaagacg tttacatcaa gctggaaaaa      540

gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa ctcggcgctg      600

actcactaca acatgattgg cttcggcacc ctggaagtga tgggcgaaaa cccggacttc      660

gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga aattaatctc ccgcgcctct      720

tatgagatgg tcgcgggtgc taccggctcg ccatacgact acccgctctc cagccgcttc      780

gatgagaacg atgcgattct ggtgatggat aacgtgctga ttccatggga aaacgtgctg      840

atctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt tgcccgtatg      900

tatccgctgc aagcctgtgt gcgcctggca gtgaaattag acttcattac ggcactgctg      960

aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc cgatctcggt     1020

gaagtggtag cgtggcgcaa caccttctgg gcattgagtg actcgatgtg ttcagaagca     1080

acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac ctatcgcgta     1140

ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt taccagtggc     1200

ctgatctatc tcccttccag tgcccgtgac ctgaataatc cgcagatcga ccagtatctg     1260

gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa gatcctcaaa     1320

ctgatgtggg atgctattgg cagcgaattt ggtggtcgtc acgaactgta tgaaatcaac     1380

tactccggta gccaggatga gattcgcctg cagtgtctgc gccaggcaca aaactccggc     1440

aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga ccaggacggc     1500

tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa gctgctgaaa     1560

taa                                                                   1563


<210>  38
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
cctctagatt aactttaaga aggagtatac atatgaaacc agaagatttc cgc              53


<210>  39
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
cggcaccctg gaagtgatgg gcgaaaaccc ggac                                   34


<210>  40
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
catcacttcc agggtgccga agccaatcat gttgtag                                37


<210>  41
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
ccggatcctt aaatcgcagc ttccatttcc ag                                     32


