                         SEQUENCE LISTING

<110>  THE TRUSTEES OF DARTMOUTH COLLEGE
 
<120>  IMMUNOSTIMULATORY CYTOKINE COMBINATION AND THERAPEUTIC USE 
       THEREOF

<130>  1143252.005413

<140>  PCT/US2022/014017
<141>  2022-01-27

<150>  63/219,620
<151>  2021-07-08

<150>  63/142,158
<151>  2021-01-27

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tailless TA99 CAR sequence

<400>  1
atggaggccc ccgcccagct gctgttcctg ctgctgctgt ggctgcccag cagcagggcc       60

gaggttcagc tccaacagtc tggggctgag cttgtgaggc caggggcctt ggtcaagttg      120

tcctgcaaaa cttctggctt caacattaaa gactactttt tacactgggt gagacagagg      180

cctgaccagg gcctggagtg gattggatgg attaatcctg ataatggtaa tactgtttat      240

gacccgaagt ttcagggcac ggccagttta acagcagaca catcctccaa cacagtctac      300

ttgcagctca gcggcctgac atctgaggac actgccgtct atttctgtac tcggagggac      360

tatacttatg aaaaggctgc tctggactac tggggtcagg gagcctcagt catcgtctcc      420

tcaggtggag gtggatcagg tggaggtgga tctggtggag gtggatctga catccagatg      480

tctcagtctc cagcctccct atctgcatct gtgggagaaa ctgtcaccat cacatgtcga      540

gcaagtggaa atatttacaa ttatttagca tggtatcagc agaaacaggg aaaatctcct      600

cacctcctgg tctatgatgc aaaaacctta gcagatggtg tgccatcaag gttcagtggc      660

agtggctcag ggacacaata ttctctcaag attagcagcc tgcagactga agattctggg      720

aattattact gtcaacattt ttggagtctt ccattcacgt tcggctcggg gacaaagttg      780

gaaataaaac gggctggtgg tggttctggt ggtggtgaac agaagctgat cagcgaggag      840

gatctgacta ctaccaagcc agtgctgcga actccctcac ctgtgcaccc taccgggaca      900

tctcagcccc agagaccaga agattgtcgg ccccgtggct cagtgaaggg gaccggattg      960

gacttcgcct gtgatattta catctgggca cccttggccg gaatctgcgt ggcccttctg     1020

ctgtccttga tcatcactct catcaatagt agaaggaaca gactccttca aagtgact       1078


<210>  2
<211>  359
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tailless TA99 CAR sequence

<400>  2

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Ser Ser Arg Ala Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val 
            20                  25                  30          


Arg Pro Gly Ala Leu Val Lys Leu Ser Cys Lys Thr Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Tyr Phe Leu His Trp Val Arg Gln Arg Pro Asp Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Trp Ile Asn Pro Asp Asn Gly Asn Thr Val Tyr 
65                  70                  75                  80  


Asp Pro Lys Phe Gln Gly Thr Ala Ser Leu Thr Ala Asp Thr Ser Ser 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Leu Ser Gly Leu Thr Ser Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Phe Cys Thr Arg Arg Asp Tyr Thr Tyr Glu Lys Ala Ala Leu 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Ala Ser Val Ile Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
145                 150                 155                 160 


Ser Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr Val Thr 
                165                 170                 175     


Ile Thr Cys Arg Ala Ser Gly Asn Ile Tyr Asn Tyr Leu Ala Trp Tyr 
            180                 185                 190         


Gln Gln Lys Gln Gly Lys Ser Pro His Leu Leu Val Tyr Asp Ala Lys 
        195                 200                 205             


Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 


Thr Gln Tyr Ser Leu Lys Ile Ser Ser Leu Gln Thr Glu Asp Ser Gly 
225                 230                 235                 240 


Asn Tyr Tyr Cys Gln His Phe Trp Ser Leu Pro Phe Thr Phe Gly Ser 
                245                 250                 255     


Gly Thr Lys Leu Glu Ile Lys Arg Ala Gly Gly Gly Ser Gly Gly Gly 
            260                 265                 270         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Thr Thr Thr Lys Pro Val 
        275                 280                 285             


Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln 
    290                 295                 300                 


Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu 
305                 310                 315                 320 


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Ile Cys 
                325                 330                 335     


Val Ala Leu Leu Leu Ser Leu Ile Ile Thr Leu Ile Asn Ser Arg Arg 
            340                 345                 350         


Asn Arg Leu Leu Gln Ser Asp 
        355                 


<210>  3
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-TYRP1 CAR sequence

<400>  3

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Ser Ser Arg Ala Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val 
            20                  25                  30          


Arg Pro Gly Ala Leu Val Lys Leu Ser Cys Lys Thr Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Tyr Phe Leu His Trp Val Arg Gln Arg Pro Asp Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Trp Ile Asn Pro Asp Asn Gly Asn Thr Val Tyr 
65                  70                  75                  80  


Asp Pro Lys Phe Gln Gly Thr Ala Ser Leu Thr Ala Asp Thr Ser Ser 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Leu Ser Gly Leu Thr Ser Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Phe Cys Thr Arg Arg Asp Tyr Thr Tyr Glu Lys Ala Ala Leu 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Ala Ser Val Ile Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
145                 150                 155                 160 


Ser Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr Val Thr 
                165                 170                 175     


Ile Thr Cys Arg Ala Ser Gly Asn Ile Tyr Asn Tyr Leu Ala Trp Tyr 
            180                 185                 190         


Gln Gln Lys Gln Gly Lys Ser Pro His Leu Leu Val Tyr Asp Ala Lys 
        195                 200                 205             


Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 


Thr Gln Tyr Ser Leu Lys Ile Ser Ser Leu Gln Thr Glu Asp Ser Gly 
225                 230                 235                 240 


Asn Tyr Tyr Cys Gln His Phe Trp Ser Leu Pro Phe Thr Phe Gly Ser 
                245                 250                 255     


Gly Thr Lys Leu Glu Ile Lys Arg Ala Gly Gly Gly Ser Gly Gly Gly 
            260                 265                 270         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Thr Thr Thr Lys Pro Val 
        275                 280                 285             


Leu Arg Thr Pro Ser Pro Val His Pro Thr Gly Thr Ser Gln Pro Gln 
    290                 295                 300                 


Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser Val Lys Gly Thr Gly Leu 
305                 310                 315                 320 


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Ile Cys 
                325                 330                 335     


Val Ala Leu Leu Leu Ser Leu Ile Ile Thr Leu Ile Asn Ser Arg Arg 
            340                 345                 350         


Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
        355                 360                 365             


Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe 
    370                 375                 380                 


Ala Ala Tyr Arg Pro Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala 
385                 390                 395                 400 


Ala Asn Leu Gln Asp Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val 
        435                 440                 445             


Tyr Asn Ala Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
    450                 455                 460                 


Gly Thr Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
465                 470                 475                 480 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
                485                 490                 495     


Gln Thr Leu Ala Pro Arg 
            500         


<210>  4
<211>  1509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  schematic of a CAR sequence

<400>  4
atggaggccc ccgcccagct gctgttcctg ctgctgctgt ggctgcccag cagcagggcc       60

gaggttcagc tccaacagtc tggggctgag cttgtgaggc caggggcctt ggtcaagttg      120

tcctgcaaaa cttctggctt caacattaaa gactactttt tacactgggt gagacagagg      180

cctgaccagg gcctggagtg gattggatgg attaatcctg ataatggtaa tactgtttat      240

gacccgaagt ttcagggcac ggccagttta acagcagaca catcctccaa cacagtctac      300

ttgcagctca gcggcctgac atctgaggac actgccgtct atttctgtac tcggagggac      360

tatacttatg aaaaggctgc tctggactac tggggtcagg gagcctcagt catcgtctcc      420

tcaggtggag gtggatcagg tggaggtgga tctggtggag gtggatctga catccagatg      480

tctcagtctc cagcctccct atctgcatct gtgggagaaa ctgtcaccat cacatgtcga      540

gcaagtggaa atatttacaa ttatttagca tggtatcagc agaaacaggg aaaatctcct      600

cacctcctgg tctatgatgc aaaaacctta gcagatggtg tgccatcaag gttcagtggc      660

agtggctcag ggacacaata ttctctcaag attagcagcc tgcagactga agattctggg      720

aattattact gtcaacattt ttggagtctt ccattcacgt tcggctcggg gacaaagttg      780

gaaataaaac gggctggtgg tggttctggt ggtggtgaac agaagctgat cagcgaggag      840

gatctgacta ctaccaagcc agtgctgcga actccctcac ctgtgcaccc taccgggaca      900

tctcagcccc agagaccaga agattgtcgg ccccgtggct cagtgaaggg gaccggattg      960

gacttcgcct gtgatattta catctgggca cccttggccg gaatctgcgt ggcccttctg     1020

ctgtccttga tcatcactct catcaatagt agaaggaaca gactccttca aagtgactac     1080

atgaacatga ctccccggag gcctgggctc actcgaaagc cttaccagcc ctacgcccct     1140

gccagagact ttgcagcgta ccgccccaga gcaaaattca gcaggagtgc agagactgct     1200

gccaacctgc aggaccccaa ccagctctac aatgagctca atctagggcg aagagaggaa     1260

tatgacgtct tggagaagaa gcgggctcgg gacccagaga tgggaggcaa acagcagagg     1320

aggaggaacc cccaggaagg cgtatacaat gcactgcaga aagacaagat ggcagaagcc     1380

tacagtgaga tcggcacaaa aggcgagagg cggagaggca aggggcacga tggcctttac     1440

cagggtctca gcactgccac caaggacacc tatgatgccc tgcatatgca gaccctggcc     1500

cctcgctga                                                             1509


<210>  5
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytokine construct

<400>  5

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala His Ser Lys 
                85                  90                  95      


Asn Phe His Phe Asp Pro Arg Asp Val Val Ser Asn Ile Asn Val Phe 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Arg Ala Lys Arg Ser Gly 
145                 150                 155                 160 


Ser Gly Glu Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu 
                165                 170                 175     


Asn Pro Gly Pro Thr Ile Gly Ala Gly Lys Gln Glu Gln Gln Thr Asp 
            180                 185                 190         


Lys Ser Asp Pro Phe His Ala Ala Ser Val Asp Thr Leu Ser Ile Gln 
        195                 200                 205             


Gly Thr Ser Leu Leu Thr Gln Ser Pro Ala Ser Leu Ser Thr Tyr Asn 
    210                 215                 220                 


Asp Gln Ser Val Ser Phe Val Leu Glu Asn Gly Cys Tyr Val Ile Asn 
225                 230                 235                 240 


Val Asp Asp Ser Gly Lys Asp Gln Glu Gln Asp Gln Val Leu Leu Arg 
                245                 250                 255     


Tyr Tyr Glu Ser Pro Cys Pro Ala Ser Gln Ser Gly Asp Gly Val Asp 
            260                 265                 270         


Gly Lys Lys Leu Met Val Asn Met Ser Pro Ile Lys Asp Thr Asp Ile 
        275                 280                 285             


Trp Leu His Ala Asn Asp Lys Asp Tyr Ser Val Glu Leu Gln Arg Gly 
    290                 295                 300                 


Asp Val Ser Pro Pro Glu Gln Ala Phe Phe Val Leu His Lys Lys Ser 
305                 310                 315                 320 


Ser Asp Phe Val Ser Phe Glu Cys Lys Asn Leu Pro Gly Thr Tyr Ile 
                325                 330                 335     


Gly Val Lys Asp Asn Gln Leu Ala Leu Val Glu Glu Lys Asp Glu Ser 
            340                 345                 350         


Cys Asn Asn Ile Met Phe Lys Leu Ser Lys Ile Gly Gly Ser Gly Gly 
        355                 360                 365             


Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Arg 
    370                 375                 380                 


Thr Gly His His His His His His 
385                 390         


<210>  6
<211>  1176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  schematic of a cytokine sequence

<400>  6
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt       60

gcccccacca gcagtagcac aaaaaagact caacttcagc ttgaacacct ccttcttgac      120

ttgcagatga ttcttaacgg gatcaacaac tataaaaacc ctaagctcac caggatgctg      180

acctttaaat tctacatgcc taaaaaggct acggagctca aacacttgca gtgtctggag      240

gaggagctta aaccgcttga agaagttctc aacctggccc atagcaagaa tttccacttc      300

gatcccagag atgttgtttc caacatcaat gtattcgtgc tcgaacttaa aggttctgag      360

acgactttca tgtgcgaata tgcagatgag acggcgacaa tcgtcgagtt tctgaacagg      420

tggatcactt tttgccaaag tataatctcc acactgacgg gaagagccaa aaggtctggc      480

tccggtgagg gcagaggaag tcttataaca tgcggtgacg tggaggagaa tcccggccct      540

actataggcg ctggaaaaca agaacaacaa actgataaaa gtgacccatt tcatgctgcg      600

tctgttgaca cattgagcat ccaaggaact tcacttttaa cacagtctcc tgcctccctg      660

agtacataca atgaccaatc tgttagtttt gttttggaga atggatgtta tgtgatcaat      720

gttgacgact ctggaaaaga ccaagagcaa gaccaggtgc tactacgcta ctatgagtct      780

ccctgtcctg caagtcaatc aggcgacggt gtggatggga agaagctgat ggtgaacatg      840

agtcccatca aagacacaga catctggctg catgccaacg acaaggacta ctccgtggag      900

cttcaaaggg gtgacgtctc gcctccggaa caggccttct tcgtccttca caaaaagtcc      960

tcggactttg tttcatttga atgcaagaat cttcctggca cttacatagg agtaaaagat     1020

aaccagctgg ctctagtgga ggagaaagat gagagctgca acaatattat gtttaagctc     1080

tcgaaaatcg gaggttctgg tggatcaggt aagcctatcc ctaaccctct cctcggtctc     1140

gattctacgc gtaccggtca tcatcaccat caccat                               1176


<210>  7
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  immunostimulatory cytokine construct comprising Super2 and IL25

<400>  7

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala His Ser Lys 
                85                  90                  95      


Asn Phe His Phe Asp Pro Arg Asp Val Val Ser Asn Ile Asn Val Phe 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Arg Ala Lys Arg Ser Gly 
145                 150                 155                 160 


Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
                165                 170                 175     


Asn Pro Gly Pro Met Tyr Gln Ala Val Ala Phe Leu Ala Met Ile Val 
            180                 185                 190         


Gly Thr His Thr Val Ser Leu Arg Ile Gln Glu Gly Cys Ser His Leu 
        195                 200                 205             


Pro Ser Cys Cys Pro Ser Lys Glu Gln Glu Pro Pro Glu Glu Trp Leu 
    210                 215                 220                 


Lys Trp Ser Ser Ala Ser Val Ser Pro Pro Glu Pro Leu Ser His Thr 
225                 230                 235                 240 


His His Ala Glu Ser Cys Arg Ala Ser Lys Asp Gly Pro Leu Asn Ser 
                245                 250                 255     


Arg Ala Ile Ser Pro Trp Ser Tyr Glu Leu Asp Arg Asp Leu Asn Arg 
            260                 265                 270         


Val Pro Gln Asp Leu Tyr His Ala Arg Cys Leu Cys Pro His Cys Val 
        275                 280                 285             


Ser Leu Gln Thr Gly Ser His Met Asp Pro Leu Gly Asn Ser Val Pro 
    290                 295                 300                 


Leu Tyr His Asn Gln Thr Val Phe Tyr Arg Arg Pro Cys His Gly Glu 
305                 310                 315                 320 


Glu Gly Thr His Arg Arg Tyr Cys Leu Glu Arg Arg Leu Tyr Arg Val 
                325                 330                 335     


Ser Leu Ala Cys Val Cys Val Arg Pro Arg Val Met Ala Gly Gly Ser 
            340                 345                 350         


Gly Gly Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser 
        355                 360                 365             


Thr Arg Thr Gly His His His His His His 
    370                 375             


<210>  8
<211>  1134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  immunostimulatory cytokine construct comprising Super2 and IL25

<400>  8
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt       60

gcccccacca gcagtagcac aaaaaagact caacttcagc ttgaacacct ccttcttgac      120

ttgcagatga ttcttaacgg gatcaacaac tataaaaacc ctaagctcac caggatgctg      180

acctttaaat tctacatgcc taaaaaggct acggagctca aacacttgca gtgtctggag      240

gaggagctta aaccgcttga agaagttctc aacctggccc atagcaagaa tttccacttc      300

gatcccagag atgttgtttc caacatcaat gtattcgtgc tcgaacttaa aggttctgag      360

acgactttca tgtgcgaata tgcagatgag acggcgacaa tcgtcgagtt tctgaacagg      420

tggatcactt tttgccaaag tataatctcc acactgacgg gaagagccaa aaggtctggc      480

tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct      540

atgtaccagg ctgttgcatt cttggcaatg atcgtgggaa cccacaccgt cagcttgcgg      600

atccaggagg gctgcagtca cttgcccagc tgctgcccca gcaaagagca agaacccccg      660

gaggagtggc tgaagtggag ctctgcatct gtgtcccccc cagagcctct gagccacacc      720

caccacgcag aatcctgcag ggccagcaag gatggccccc tcaacagcag ggccatctct      780

ccttggagct atgagttgga cagggacttg aatcgggtcc cccaggacct gtaccacgct      840

cgatgcctgt gcccacactg cgtcagccta cagacaggct cccacatgga cccgctgggc      900

aactccgtcc cactttacca caaccagacg gtcttctacc ggcggccatg ccatggcgag      960

gaaggtaccc atcgccgcta ctgcttggag cgcaggctct accgagtctc cttggcttgt     1020

gtgtgtgtgc ggccccgggt catggctgga ggttctggtg gatcaggtaa gcctatccct     1080

aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccat           1134


<210>  9
<211>  461
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary anti-B7H6 (TZ47) CAR construct

<400>  9

Met Glu Trp Thr Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Met Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Thr Gly Tyr Thr Phe 
        35                  40                  45              


Thr Gly Tyr Trp Ile Glu Trp Ile Lys Gln Arg Pro Gly His Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Leu Pro Gly Thr Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Lys Ala Thr Phe Thr Ala Asp Thr Ser Ser Asn 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Thr Glu Asp Ser Ala Ile 
            100                 105                 110         


Tyr Tyr Cys Ala Ile Pro Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Ser Val Thr Val Ser Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Phe Asp Ile Lys Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Met Tyr Ala Ser Leu Gly Glu Arg Val Thr Ile Thr Cys Lys 
                165                 170                 175     


Ala Ser Gln Asp Ile Asn Ser Tyr Leu Ser Trp Phe Gln Gln Lys Pro 
            180                 185                 190         


Gly Lys Ser Pro Lys Thr Leu Ile Tyr Arg Ala Asn Arg Leu Val Asp 
        195                 200                 205             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Gln Asp Tyr Ser 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Glu Tyr Glu Asp Met Gly Ile Tyr Tyr Cys 
225                 230                 235                 240 


Leu Gln Tyr Asp Glu Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Ala Ser Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            260                 265                 270         


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
305                 310                 315                 320 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                325                 330                 335     


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Leu Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460     


<210>  10
<211>  1383
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary anti-B7H6 (TZ47) CAR construct

<400>  10
atggaatgga cctgggtctt tctcttcctc ctgtcagtaa ctgcaggtgt ccactcccag       60

gttcagctgc agcagtctgg agctgagctg atgaagcctg gggcctcagt gaagctttcc      120

tgcaaggcta ctggctacac attcactggc tactggatag agtggataaa gcagaggcct      180

ggacatggcc ttgagtggat tggagagatt ttacctggaa ctggtagtac taactacaat      240

gagaagttca agggcaaggc cacattcact gcagatacat cctccaacac agcctacatg      300

caactcagca gcctgacaac tgaggactct gccatctatt actgtgcaat cccggggcct      360

atggactact ggggtcaagg aacctcagtc accgtctcct cagccggcgg aggcggatca      420

ggaggaggag gatcaggcgg aggaggatca gaattcgaca tcaagatgac ccagtctcca      480

tcttccatgt atgcatctct aggagagaga gtcactatca cttgcaaggc gagtcaggac      540

attaatagct atttaagctg gttccagcag aaaccaggga aatctcctaa gaccctgatc      600

tatcgtgcaa acagattggt agatggggtc ccatcaaggt tcagtggcag tggatctggg      660

caagattatt ctctcaccat cagcagcctg gagtatgaag atatgggaat ttattattgt      720

ctacagtatg atgagtttcc gtacacgttc ggagggggga ccaagctgga aataaaagct      780

agcgtgaaag ggaaacacct ttgtccaagt cccctatttc ccggaccttc taagcccttt      840

tgggtgctgg tggtggttgg tggagtcctg gcttgctata gcttgctagt aacagtggcc      900

tttattattt tctgggtgag gagtaagagg agcaggctcc tgcacagtga ctacatgaac      960

atgactcccc gccgccccgg gcccacccgc aagcattacc agccctatgc cccaccacgc     1020

gacttcgcag cctatcgctc caagcttaga gtgaagttca gcaggagcgc agacgccccc     1080

gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag     1140

tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgagaagg     1200

aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc ggaggcctac     1260

agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg cctttaccag     1320

ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc cctgccccct     1380

cgc                                                                   1383


<210>  11
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary anti-Rae1 CAR construct comprising mouse NKG2D full 
       length type 2 membrane protein

<400>  11

Met Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn Leu Gln 
1               5                   10                  15      


Asp Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          


Tyr Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              


Lys Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn Ala Leu 
    50                  55                  60                  


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr Lys Gly 
65                  70                  75                  80  


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                85                  90                  95      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr Leu Ala 
            100                 105                 110         


Pro Arg Glu Phe Ala Leu Ile Arg Asp Arg Lys Ser His His Ser Glu 
        115                 120                 125             


Met Ser Lys Cys His Asn Tyr Asp Leu Lys Pro Ala Lys Trp Asp Thr 
    130                 135                 140                 


Ser Gln Glu Gln Gln Lys Gln Arg Leu Ala Leu Thr Thr Ser Gln Pro 
145                 150                 155                 160 


Gly Glu Asn Gly Ile Ile Arg Gly Arg Tyr Pro Ile Glu Lys Leu Lys 
                165                 170                 175     


Ile Ser Pro Met Phe Val Val Arg Val Leu Ala Ile Ala Leu Ala Ile 
            180                 185                 190         


Arg Phe Thr Leu Asn Thr Leu Met Trp Leu Ala Ile Phe Lys Glu Thr 
        195                 200                 205             


Phe Gln Pro Val Leu Cys Asn Lys Glu Val Pro Val Ser Ser Arg Glu 
    210                 215                 220                 


Gly Tyr Cys Gly Pro Cys Pro Asn Asn Trp Ile Cys His Arg Asn Asn 
225                 230                 235                 240 


Cys Tyr Gln Phe Phe Asn Glu Glu Lys Thr Trp Asn Gln Ser Gln Ala 
                245                 250                 255     


Ser Cys Leu Ser Gln Asn Ser Ser Leu Leu Lys Ile Tyr Ser Lys Glu 
            260                 265                 270         


Glu Gln Asp Phe Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu 
        275                 280                 285             


Val Gln Ile Pro Ala Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ser 
    290                 295                 300                 


Leu Ser Tyr Asn Gln Leu Thr Leu Val Glu Ile Pro Lys Gly Ser Cys 
305                 310                 315                 320 


Ala Val Tyr Gly Ser Ser Phe Lys Ala Tyr Thr Glu Asp Cys Ala Asn 
                325                 330                 335     


Leu Asn Thr Tyr Ile Cys Met Lys Arg Ala Val 
            340                 345         


<210>  12
<211>  1044
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary anti-Rae1 CAR construct

<400>  12
atgagagcaa aattcagcag gagtgcagag actgctgcca acctgcagga ccccaaccag       60

ctctacaatg agctcaatct agggcgaaga gaggaatatg acgtcttgga gaagaagcgg      120

gctcgggatc cagagatggg aggcaaacag cagaggagga ggaaccccca ggaaggcgta      180

tacaatgcac tgcagaaaga caagatggca gaagcctaca gtgagatcgg cacaaaaggc      240

gagaggcgga gaggcaaggg gcacgatggc ctttaccagg gtctcagcac tgccaccaag      300

gacacctatg atgccctgca tatgcagacc ctggcccctc gcgaattcgc attgattcgt      360

gatcgaaagt ctcatcactc agagatgagc aaatgccata attacgacct caagccagca      420

aagtgggata cttctcaaga acaacagaaa caaagattag cactaactac cagtcaacct      480

ggagaaaatg gtatcataag aggaagatac cctatagaaa aactcaaaat atctccaatg      540

ttcgttgttc gagtccttgc tatagccttg gcaattcgat tcacccttaa cacattgatg      600

tggcttgcca ttttcaaaga gacgtttcag ccagtattgt gcaacaagga agtcccagtt      660

tcctcaagag agggctactg tggcccatgc cctaacaact ggatatgtca cagaaacaac      720

tgttaccaat tttttaatga agagaaaacc tggaaccaga gccaagcttc ctgtttgtct      780

caaaattcca gccttctgaa gatatacagt aaagaagaac aggatttctt aaagctggtt      840

aagtcctatc actggatggg actggtccag atcccagcaa atggctcctg gcagtgggaa      900

gatggctcct ctctctcata caatcagtta actctggtgg aaataccaaa aggatcctgt      960

gctgtctatg gctcaagctt taaggcttac acagaagact gtgcaaatct aaacacgtac     1020

atctgcatga aaagggcggt gtaa                                            1044


