                         SEQUENCE LISTING

<110>  Umoja Biopharma, Inc.
       Scharenberg, Andrew
       Crisman, Ryan
       Nicolai, Christopher
       Sullivan, Alessandra
       Michels, Kathryn
       Ryu, Byoung
       Green, Shon
       Beitz, Laurie
       Hernandez Lopez, Susana
 
<120>  LENTIVIRUS FOR GENERATING CELLS EXPRESSING ANTI-CD19 CHIMERIC 
       ANTIGEN RECEPTOR

<130>  UMOJ-024/01WO 337798-2211

<150>  63/142,347
<151>  2021-01-27

<150>  63/185,765
<151>  2021-05-07

<160>  144   

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  2
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-CD3 scFv

<400>  2

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu 
        115                 120                 125             


Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu 
    130                 135                 140                 


Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp 
145                 150                 155                 160 


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn 
                165                 170                 175     


Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg Phe 
            180                 185                 190         


Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp 
        195                 200                 205             


Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr 
    210                 215                 220                 


Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr 
225                 230                 235                 240 


Val Ser Ser Ala Ala Ala Lys Pro 
                245             


<210>  3
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  3

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Ser Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Ser Asp 
        35                  40                  45  


<210>  4
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - CD4 TM, cytoplasmic tail and T2A linker construct

<400>  4

Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile 
1               5                   10                  15      


Gly Leu Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Gln 
            20                  25                  30          


Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
        35                  40                  45              


Glu Asn Pro Gly Pro 
    50              


<210>  5
<211>  511
<212>  PRT
<213>  Cocal virus

<400>  5

Asn Phe Leu Leu Leu Thr Phe Ile Val Leu Pro Leu Cys Ser His Ala 
1               5                   10                  15      


Lys Phe Ser Ile Val Phe Pro Gln Ser Gln Lys Gly Asn Trp Lys Asn 
            20                  25                  30          


Val Pro Ser Ser Tyr His Tyr Cys Pro Ser Ser Ser Asp Gln Asn Trp 
        35                  40                  45              


His Asn Asp Leu Leu Gly Ile Thr Met Lys Val Lys Met Pro Lys Thr 
    50                  55                  60                  


His Lys Ala Ile Gln Ala Asp Gly Trp Met Cys His Ala Ala Lys Trp 
65                  70                  75                  80  


Ile Thr Thr Cys Asp Phe Arg Trp Tyr Gly Pro Lys Tyr Ile Thr His 
                85                  90                  95      


Ser Ile His Ser Ile Gln Pro Thr Ser Glu Gln Cys Lys Glu Ser Ile 
            100                 105                 110         


Lys Gln Thr Lys Gln Gly Thr Trp Met Ser Pro Gly Phe Pro Pro Gln 
        115                 120                 125             


Asn Cys Gly Tyr Ala Thr Val Thr Asp Ser Val Ala Val Val Val Gln 
    130                 135                 140                 


Ala Thr Pro His His Val Leu Val Asp Glu Tyr Thr Gly Glu Trp Ile 
145                 150                 155                 160 


Asp Ser Gln Phe Pro Asn Gly Lys Cys Glu Thr Glu Glu Cys Glu Thr 
                165                 170                 175     


Val His Asn Ser Thr Val Trp Tyr Ser Asp Tyr Lys Val Thr Gly Leu 
            180                 185                 190         


Cys Asp Ala Thr Leu Val Asp Thr Glu Ile Thr Phe Phe Ser Glu Asp 
        195                 200                 205             


Gly Lys Lys Glu Ser Ile Gly Lys Pro Asn Thr Gly Tyr Arg Ser Asn 
    210                 215                 220                 


Tyr Phe Ala Tyr Glu Lys Gly Asp Lys Val Cys Lys Met Asn Tyr Cys 
225                 230                 235                 240 


Lys His Ala Gly Val Arg Leu Pro Ser Gly Val Trp Phe Glu Phe Val 
                245                 250                 255     


Asp Gln Asp Val Tyr Ala Ala Ala Lys Leu Pro Glu Cys Pro Val Gly 
            260                 265                 270         


Ala Thr Ile Ser Ala Pro Thr Gln Thr Ser Val Asp Val Ser Leu Ile 
        275                 280                 285             


Leu Asp Val Glu Arg Ile Leu Asp Tyr Ser Leu Cys Gln Glu Thr Trp 
    290                 295                 300                 


Ser Lys Ile Arg Ser Lys Gln Pro Val Ser Pro Val Asp Leu Ser Tyr 
305                 310                 315                 320 


Leu Ala Pro Lys Asn Pro Gly Thr Gly Pro Ala Phe Thr Ile Ile Asn 
                325                 330                 335     


Gly Thr Leu Lys Tyr Phe Glu Thr Arg Tyr Ile Arg Ile Asp Ile Asp 
            340                 345                 350         


Asn Pro Ile Ile Ser Lys Met Val Gly Lys Ile Ser Gly Ser Gln Thr 
        355                 360                 365             


Glu Arg Glu Leu Trp Thr Glu Trp Phe Pro Tyr Glu Gly Val Glu Ile 
    370                 375                 380                 


Gly Pro Asn Gly Ile Leu Lys Thr Pro Thr Gly Tyr Lys Phe Pro Leu 
385                 390                 395                 400 


Phe Met Ile Gly His Gly Met Leu Asp Ser Asp Leu His Lys Thr Ser 
                405                 410                 415     


Gln Ala Glu Val Phe Glu His Pro His Leu Ala Glu Ala Pro Lys Gln 
            420                 425                 430         


Leu Pro Glu Glu Glu Thr Leu Phe Phe Gly Asp Thr Gly Ile Ser Lys 
        435                 440                 445             


Asn Pro Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser Thr 
    450                 455                 460                 


Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr Val 
465                 470                 475                 480 


Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser Asn 
                485                 490                 495     


Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys 
            500                 505                 510     


<210>  6
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding CD8 signal peptide

<400>  6
atggcactgc ctgtgacagc cctgctgctg ccactggccc tgctgctgca cgcagcacgc       60

cca                                                                     63


<210>  7
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding anti-cd3 scFv construct

<400>  7
gatatccaga tgacccagtc cccaagctcc ctgagcgcct ccgtgggcga ccgggtgaca       60

atcacctgca gcgcctctag ctccgtgtcc tacatgaact ggtatcagca gacacctggc      120

aaggccccaa agagatggat ctacgatacc agcaagctgg cctccggcgt gccttctagg      180

ttttctggca gcggctccgg cacagattat acattcacca tctctagcct gcagccagag      240

gacatcgcca cctactattg ccagcagtgg tcctctaatc cctttacatt cggccagggc      300

accaagctgc agatcacaag aacctctgga ggaggaggaa gcggaggagg aggatccggc      360

ggcggcggct ctcaggtgca gctggtgcag agcggaggag gagtggtgca gccaggcaga      420

agcctgaggc tgtcctgtaa ggcctctggc tacacattca ccagatatac aatgcactgg      480

gtgaggcagg caccaggcaa gggactggag tggatcggct acatcaaccc ctccaggggc      540

tacaccaact ataatcagaa ggtgaaggat cggttcacca tcagcaggga caactccaag      600

aataccgcct tcctgcagat ggacagcctg aggccagagg ataccggcgt gtacttttgc      660

gcccggtact atgacgatca ctactgtctg gattattggg gccagggaac accagtgacc      720

gtgagctccg ccgcagcaaa gcct                                             744


<210>  8
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding cd8 hinge

<400>  8
accacaaccc ctgccccaag gccacctaca cccgccccta ccatcgcctc tcagccactg       60

agcctgaggc cagaggcatc caggcctgcc gcaggggggg ccgtgcacac ccggggcctg      120

gactttgcct ctgat                                                       135


<210>  9
<211>  159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding cd tm, cytoplasmic tail and
       T2a linker construct

<400>  9
atggcactga tcgtgctggg aggagtggca ggactgctgc tgttcatcgg actgggcatc       60

ttcttttgcg tgcgctgtag gcaccggaga aggcagggat ctggagaggg aaggggaagc      120

ctgctgacat gcggcgacgt ggaggagaac ccaggacca                             159


<210>  10
<211>  1533
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding cocal env

<400>  10
aattttctgc tgctgacctt catcgtgctg cctctgtgca gccacgccaa gttttccatc       60

gtgttcccac agtcccagaa gggcaactgg aagaatgtgc cctctagcta ccactattgc      120

ccttcctcta gcgaccagaa ctggcacaat gatctgctgg gcatcacaat gaaggtgaag      180

atgcccaaga cccacaaggc catccaggca gatggatgga tgtgccacgc agccaagtgg      240

atcacaacct gtgactttcg gtggtacggc cccaagtata tcacacactc catccactct      300

atccagccta cctccgagca gtgcaaggag tctatcaagc agacaaagca gggcacctgg      360

atgagccctg gcttcccacc ccagaactgt ggctacgcca cagtgaccga ctccgtggca      420

gtggtggtgc aggcaacacc tcaccacgtg ctggtggatg agtataccgg cgagtggatc      480

gacagccagt ttccaaacgg caagtgcgag acagaggagt gtgagaccgt gcacaattct      540

acagtgtggt acagcgatta taaggtgaca ggcctgtgcg acgccaccct ggtggataca      600

gagatcacct tcttttctga ggacggcaag aaggagagca tcggcaagcc caacaccggc      660

tacagatcca attacttcgc ctatgagaag ggcgataagg tgtgcaagat gaattattgt      720

aagcacgccg gggtgcggct gcctagcggc gtgtggtttg agttcgtgga ccaggacgtg      780

tacgcagcag caaagctgcc tgagtgccca gtgggagcaa ccatctccgc cccaacacag      840

acctccgtgg acgtgtctct gatcctggat gtggagcgca tcctggacta cagcctgtgc      900

caggagacct ggagcaagat ccggtccaag cagcccgtgt cccctgtgga cctgtcttac      960

ctggcaccaa agaacccagg aaccggacca gcctttacaa tcatcaatgg caccctgaag     1020

tacttcgaga cccgctatat ccggatcgac atcgataacc ctatcatcag caagatggtg     1080

ggcaagatct ctggcagcca gacagagaga gagctgtgga ccgagtggtt cccttacgag     1140

ggcgtggaga tcggcccaaa tggcatcctg aagacaccaa ccggctataa gtttcccctg     1200

ttcatgatcg gccacggcat gctggacagc gatctgcaca agacctccca ggccgaggtg     1260

tttgagcacc cacacctggc agaggcacca aagcagctgc ctgaggagga gacactgttc     1320

tttggcgata ccggcatctc taagaacccc gtggagctga tcgagggctg gttttcctct     1380

tggaagagca cagtggtgac cttctttttc gccatcggcg tgttcatcct gctgtacgtg     1440

gtggccagaa tcgtgatcgc cgtgagatac aggtatcagg gctccaacaa taagaggatc     1500

tataatgaca tcgagatgtc tcgcttccgg aag                                  1533


<210>  11
<211>  17
<212>  PRT
<213>  Unknown

<220>
<223>  Gaussia species luciferase

<400>  11

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala 
    


<210>  12
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-cd3 scFv construct

<400>  12

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu 
        115                 120                 125             


Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu 
    130                 135                 140                 


Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp 
145                 150                 155                 160 


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn 
                165                 170                 175     


Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg Phe 
            180                 185                 190         


Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp 
        195                 200                 205             


Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr 
    210                 215                 220                 


Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr 
225                 230                 235                 240 


Val Ser Ser Ala Ser 
                245 


<210>  13
<211>  62
<212>  PRT
<213>  Cocal virus

<400>  13

Gly Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser Thr Val 
1               5                   10                  15      


Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr Val Val 
            20                  25                  30          


Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser Asn Asn 
        35                  40                  45              


Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys 
    50                  55                  60          


<210>  14
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding a Gaussia species 
       luciferase signal peptide

<400>  14
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc c                51


<210>  15
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding anti-cd3 scFv construct

<400>  15
gacatccaga tgacccagtc tcctagcagc ctcagcgcta gcgtgggcga tagagtgacc       60

atcacatgta gcgccagcag cagcgtgtcc tacatgaact ggtaccagca aacacctgga      120

aaggccccta aaaggtggat ctatgacaca tctaagctgg cttctggagt gccatctaga      180

ttttctggca gcggctccgg cactgattat acattcacca tcagcagcct gcagcccgag      240

gatatcgcca cctactactg tcagcagtgg tcctctaatc ccttcacctt cggccagggc      300

accaagctgc agatcaccag aaccagcggc gggggaggaa gcggcggggg aggatctggc      360

ggcggcggca gccaggtgca gctggtgcag agcggcggcg gcgtggtgca acctggcaga      420

agcctgagac tgagctgcaa ggcctctggc tacaccttca cccggtacac catgcattgg      480

gtgcggcagg cccctggcaa gggcctggaa tggattggat acatcaaccc cagcagaggc      540

tacaccaact acaaccagaa ggtgaaggac agattcacaa tttctcggga caacagcaag      600

aataccgcct tcctgcaaat ggactccctg cgcccagaag ataccggcgt gtacttctgc      660

gctagatatt acgacgacca ctactgcctg gactactggg gccagggcac ccctgtgacc      720

gtgtccagcg cctcc                                                       735


<210>  16
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding short hinge-TM-CT from 
       Cocal Env

<400>  16
ggagtggaac tgatcgaggg ctggttcagc agctggaaaa gcaccgtggt tacattcttt       60

ttcgccatcg gcgtgttcat cctgctgtac gtggtcgcca gaattgtgat cgccgtgcgg      120

tatagatacc agggcagcaa caacaagcgg atctacaacg acatcgagat gagcagattc      180

agaaag                                                                 186


<210>  17

<400>  17
000

<210>  18
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-cd3 scFv construct

<400>  18
000
<210>  19
<211>  93
<212>  PRT
<213>  Cocal virus

<400>  19

Ser Gly Phe Glu His Pro His Leu Ala Glu Ala Pro Lys Gln Leu Pro 
1               5                   10                  15      


Glu Glu Glu Thr Leu Phe Phe Gly Asp Thr Gly Ile Ser Lys Asn Pro 
            20                  25                  30          


Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser Thr Val Val 
        35                  40                  45              


Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr Val Val Ala 
    50                  55                  60                  


Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser Asn Asn Lys 
65                  70                  75                  80  


Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys 
                85                  90              


<210>  20

<400>  20
000

<210>  21
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Polynucleotide encoding anti-cd3 scFv construct

<400>  21
000

<210>  22
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding long hinge-TM-CT from Cocal
       Env

<400>  22
tccggattcg agcaccccca cctggccgag gcccctaagc agctgcctga agaagagaca       60

ctgtttttcg gagataccgg catcagcaaa aaccccgtgg agctgatcga gggctggttc      120

agctcttgga agagcaccgt ggtcacattc tttttcgcca tcggcgtctt tatcctgctg      180

tacgtggtag ccagaatcgt gatcgccgtg cggtacagat accagggcag caacaacaag      240

cggatctaca acgacatcga gatgagccgg ttcagaaag                             279


<210>  23

<400>  23
000

<210>  24
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Anti-CD3 scFv construct

<400>  24
000
<210>  25
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - human Glycophorin A ecto-TM_HIV Env CT construct

<400>  25

Ser Gly Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly 
1               5                   10                  15      


Ser Thr Lys Gly Pro Glu Ile Thr Leu Ile Ile Phe Gly Val Met Ala 
            20                  25                  30          


Gly Val Ile Gly Thr Ile Leu Leu Ile Ser Tyr Gly Ile Arg Arg Leu 
        35                  40                  45              


Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu 
    50                  55                  60                  


Lys Asn Ser Ala Val Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala 
65                  70                  75                  80  


Glu Gly Thr Asp Arg Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala 
                85                  90                  95      


Ile Arg His Ile Pro Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu 
            100                 105                 110         


Leu 
    


<210>  26

<400>  26
000

<210>  27
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding anti-cd3 scFv

<400>  27
000

<210>  28
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding human Glycophorin A 
       ecto-TM_HIV Env CT construct

<400>  28
tccggaggat ctacaagcgg ctctggcaag cctggcagcg gagaaggcag caccaagggc       60

cctgagatca cactgatcat cttcggcgtg atggccggcg tcatcggcac catcctgctg      120

atcagctacg gcatcagaag actggctctg aagtactggt ggaatctgct gcaatactgg      180

agccaggagc tgaaaaacag cgccgtgtcc ctgctcaacg ccaccgccat cgccgtggcc      240

gagggcaccg acagagtgat cgaggtggtg cagggagcct gcagagctat tcggcacatc      300

cccagacgga tcaggcaggg cctggaaaga atcctgctg                             339


<210>  29

<400>  29
000

<210>  30
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-cd3 scFv construct

<400>  30
000
<210>  31
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - 218 linker_HIV Env-TM-CT construct

<400>  31

Ser Gly Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly 
1               5                   10                  15      


Ser Thr Lys Gly Asn Trp Leu Trp Tyr Ile Arg Ile Phe Ile Ile Ile 
            20                  25                  30          


Val Gly Ser Leu Ile Gly Leu Arg Ile Val Phe Ala Val Leu Ser Leu 
        35                  40                  45              


Val Asn Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln 
    50                  55                  60                  


Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn Ala 
65                  70                  75                  80  


Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val Val 
                85                  90                  95      


Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg Gln 
            100                 105                 110         


Gly Leu Glu Arg Ile Leu Leu 
        115                 


<210>  32

<400>  32
000

<210>  33
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding anti-cd3 scFv construct

<400>  33
000

<210>  34
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding 218 linker_HIV Env-TM-CT 
       construct

<400>  34
tccggaggaa gcaccagcgg ctctggcaag cctggcagcg gcgagggctc taccaagggc       60

aattggctgt ggtacatcag aatcttcatc atcatcgtgg gcagcctgat cggcctgaga      120

atcgtgttcg ccgtgctgag cctggtgaac cggggctggg aagctctgaa gtactggtgg      180

aacctgctgc aatactggtc ccaggagctg aaaaacagcg ctgtgtccct gctcaacgcc      240

accgccatcg ccgtcgccga gggaacagac agagtgatcg aggtggtgca gggagcctgc      300

agagccattc ggcacatccc cagacgcatc agacagggcc tggaaagaat cctgctg         357


<210>  35
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MND promoter

<400>  35
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgct agc             353


<210>  36
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-cd3 scFv construct

<400>  36
000
<210>  37
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - G4Sx3 linker_HIV Env-TM-CT construct

<400>  37

Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Tyr Ile Arg Ile Phe Ile Ile Ile Val Gly Ser Leu Ile Gly Leu 
            20                  25                  30          


Arg Ile Val Phe Ala Val Leu Ser Leu Val Asn Arg Gly Trp Glu Ala 
        35                  40                  45              


Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys 
    50                  55                  60                  


Asn Ser Ala Val Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu 
65                  70                  75                  80  


Gly Thr Asp Arg Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile 
                85                  90                  95      


Arg His Ile Pro Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu 
            100                 105                 110         


<210>  38

<400>  38
000

<210>  39
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynulceotide encoding anti-cd3 scFv construct

<400>  39
000

<210>  40
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding G4Sx3 linker_HIV Env-TM-CT 
       construct

<400>  40
tccggaggcg gtggaggctc tggtggcgga gggagcggtg gcggaggcag ctacatcaga       60

atcttcatca tcatcgtggg cagcctgatc ggcctgagaa tcgtgttcgc cgttctgagc      120

ctggtgaacc ggggctggga agccctgaag tactggtgga atctgctcca gtactggtct      180

caggagctga agaacagcgc cgtgtccctg ctgaacgcta cagctatcgc cgtcgccgag      240

ggcaccgaca gagtgatcga ggtggtgcag ggcgcctgca gagccatccg gcacatccct      300

agaaggattc ggcaaggcct ggaaagaatc ctgctg                                336


<210>  41

<400>  41
000

<210>  42
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-cd3 scFv construct

<400>  42
000
<210>  43
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Short hinge-TM-CT from Cocal Env_T2A construct

<400>  43

Gly Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser Thr Val 
1               5                   10                  15      


Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr Val Val 
            20                  25                  30          


Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser Asn Asn 
        35                  40                  45              


Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys Gly Ser 
    50                  55                  60                  


Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn 
65                  70                  75                  80  


Pro Gly Pro 
            


<210>  44
<211>  0
<212>  PRT
<213>  Cocal virus

<400>  44
000
<210>  45

<400>  45
000

<210>  46
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding anti-cd3 scFv construct

<400>  46
000

<210>  47
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding Short hinge-TM-CT from 
       Cocal Env_T2A construct

<400>  47
ggagtggaac tgatcgaggg ctggttcagc agctggaaaa gcaccgtggt tacattcttt       60

ttcgccatcg gcgtgttcat cctgctgtac gtggtcgcca gaattgtgat cgccgtgcgg      120

tatagatacc agggcagcaa caacaagcgg atctacaacg acatcgagat gagcagattc      180

agaaagggat ctggagaggg aaggggaagc ctgctgacat gcggcgacgt ggaggagaac      240

ccaggacca                                                              249


<210>  48
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding Cocal Env

<400>  48
000

<210>  49
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - CD19 CAR_FRB_RACR lentiviral vector construct

<400>  49

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly 
            260                 265                 270         


Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly 
        275                 280                 285             


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    290                 295                 300                 


Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
    450                 455                 460                 


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
465                 470                 475                 480 


Pro Gly Pro Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
                485                 490                 495     


Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
            500                 505                 510         


Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
        515                 520                 525             


Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
    530                 535                 540                 


Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp 
545                 550                 555                 560 


Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly Ser Gly Ala 
                565                 570                 575     


Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro 
            580                 585                 590         


Gly Pro Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly 
        595                 600                 605             


Ala Leu His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly 
    610                 615                 620                 


Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr 
625                 630                 635                 640 


Thr Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg 
                645                 650                 655     


Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly 
            660                 665                 670         


Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu 
        675                 680                 685             


Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile 
    690                 695                 700                 


Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu 
705                 710                 715                 720 


Gly Glu Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu 
                725                 730                 735     


Glu Ala Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu 
            740                 745                 750         


Leu Cys Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr 
        755                 760                 765             


Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser 
    770                 775                 780                 


Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp 
785                 790                 795                 800 


Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly 
                805                 810                 815     


Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro 
            820                 825                 830         


Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr Gly Ser Gly 
        835                 840                 845             


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
    850                 855                 860                 


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
865                 870                 875                 880 


Leu Leu Leu His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His 
                885                 890                 895     


Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val 
            900                 905                 910         


Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg 
        915                 920                 925             


Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg 
    930                 935                 940                 


Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly 
945                 950                 955                 960 


Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe 
                965                 970                 975     


Arg Arg Ile Ser Lys Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu 
            980                 985                 990         


Leu Val Gly Leu Ser Gly Ala Phe  Gly Phe Ile Ile Leu  Val Tyr Leu 
        995                 1000                 1005             


Leu Ile  Asn Cys Arg Asn Thr  Gly Pro Trp Leu Lys  Lys Val Leu 
    1010                 1015                 1020             


Lys Cys  Asn Thr Pro Asp Pro  Ser Lys Phe Phe Ser  Gln Leu Ser 
    1025                 1030                 1035             


Ser Glu  His Gly Gly Asp Val  Gln Lys Trp Leu Ser  Ser Pro Phe 
    1040                 1045                 1050             


Pro Ser  Ser Ser Phe Ser Pro  Gly Gly Leu Ala Pro  Glu Ile Ser 
    1055                 1060                 1065             


Pro Leu  Glu Val Leu Glu Arg  Asp Lys Val Thr Gln  Leu Leu Leu 
    1070                 1075                 1080             


Gln Gln  Asp Lys Val Pro Glu  Pro Ala Ser Leu Ser  Ser Asn His 
    1085                 1090                 1095             


Ser Leu  Thr Ser Cys Phe Thr  Asn Gln Gly Tyr Phe  Phe Phe His 
    1100                 1105                 1110             


Leu Pro  Asp Ala Leu Glu Ile  Glu Ala Cys Gln Val  Tyr Phe Thr 
    1115                 1120                 1125             


Tyr Asp  Pro Tyr Ser Glu Glu  Asp Pro Asp Glu Gly  Val Ala Gly 
    1130                 1135                 1140             


Ala Pro  Thr Gly Ser Ser Pro  Gln Pro Leu Gln Pro  Leu Ser Gly 
    1145                 1150                 1155             


Glu Asp  Asp Ala Tyr Cys Thr  Phe Pro Ser Arg Asp  Asp Leu Leu 
    1160                 1165                 1170             


Leu Phe  Ser Pro Ser Leu Leu  Gly Gly Pro Ser Pro  Pro Ser Thr 
    1175                 1180                 1185             


Ala Pro  Gly Gly Ser Gly Ala  Gly Glu Glu Arg Met  Pro Pro Ser 
    1190                 1195                 1200             


Leu Gln  Glu Arg Val Pro Arg  Asp Trp Asp Pro Gln  Pro Leu Gly 
    1205                 1210                 1215             


Pro Pro  Thr Pro Gly Val Pro  Asp Leu Val Asp Phe  Gln Pro Pro 
    1220                 1225                 1230             


Pro Glu  Leu Val Leu Arg Glu  Ala Gly Glu Glu Val  Pro Asp Ala 
    1235                 1240                 1245             


Gly Pro  Arg Glu Gly Val Ser  Phe Pro Trp Ser Arg  Pro Pro Gly 
    1250                 1255                 1260             


Gln Gly  Glu Phe Arg Ala Leu  Asn Ala Arg Leu Pro  Leu Asn Thr 
    1265                 1270                 1275             


Asp Ala  Tyr Leu Ser Leu Gln  Glu Leu Gln Gly Gln  Asp Pro Thr 
    1280                 1285                 1290             


His Leu  Val 
    1295     


<210>  50
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  50

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  51
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - VL-linker-VH construct

<400>  51

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  52
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IgG4 linker-CD28 TM construct

<400>  52

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val 
1               5                   10                  15      


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
            20                  25                  30          


Val Ala Phe Ile Ile Phe Trp Val 
        35                  40  


<210>  53
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  53

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  54
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  54

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  55
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A synthetic construct

<400>  55

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  56
<211>  89
<212>  PRT
<213>  Homo sapiens

<400>  56

Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly 
1               5                   10                  15      


Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala 
            20                  25                  30          


Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln 
        35                  40                  45              


Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr 
    50                  55                  60                  


Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr 
65                  70                  75                  80  


Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  


<210>  57
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide-FKBP12 synthetic construct

<400>  57

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu 
        115                 120                 125     


<210>  58
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2R gamma TM-Cytoplasmic domain synthetic construct

<400>  58

Gly Glu Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu 
1               5                   10                  15      


Glu Ala Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu 
            20                  25                  30          


Leu Cys Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr 
        35                  40                  45              


Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser 
    50                  55                  60                  


Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp 
65                  70                  75                  80  


Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly 
                85                  90                  95      


Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro 
            100                 105                 110         


Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
        115                 120                 125 


<210>  59
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide-FRB synthetic construct

<400>  59

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys 
        


<210>  60
<211>  315
<212>  PRT
<213>  Homo sapiens

<400>  60

Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser 
1               5                   10                  15      


Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg 
            20                  25                  30          


Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp 
        35                  40                  45              


Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly Asp Val 
    50                  55                  60                  


Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser Pro Gly 
65                  70                  75                  80  


Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg Asp Lys 
                85                  90                  95      


Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro Ala Ser 
            100                 105                 110         


Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln Gly Tyr 
        115                 120                 125             


Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys Gln Val 
    130                 135                 140                 


Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu Gly Val 
145                 150                 155                 160 


Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro Leu Ser 
                165                 170                 175     


Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp Leu Leu 
            180                 185                 190         


Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser Thr Ala 
        195                 200                 205             


Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser Leu Gln 
    210                 215                 220                 


Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro Pro Thr 
225                 230                 235                 240 


Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu Leu Val 
                245                 250                 255     


Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg Glu Gly 
            260                 265                 270         


Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe Arg Ala 
        275                 280                 285             


Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser Leu Gln 
    290                 295                 300                 


Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
305                 310                 315 


<210>  61
<211>  3891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding CD19 CAR_FRB_RACR lentiviral vector 
       construct

<400>  61
atgctgctgc tggtgacctc cctgctgctg tgcgagctgc ctcacccagc ctttctgctg       60

atccccgaca tccagatgac acagaccaca agctccctgt ctgccagcct gggcgacaga      120

gtgaccatct cctgtagggc ctctcaggat atcagcaagt acctgaactg gtatcagcag      180

aagccagatg gcacagtgaa gctgctgatc taccacacct ccaggctgca ctctggagtg      240

ccaagccggt tctccggatc tggaagcggc accgactatt ccctgacaat ctctaacctg      300

gagcaggagg atatcgccac atacttttgc cagcagggca ataccctgcc atatacattc      360

ggcggaggaa ccaagctgga gatcaccgga tccacatctg gaagcggcaa gccaggaagc      420

ggagagggat ccacaaaggg agaggtgaag ctgcaggaga gcggaccagg actggtggca      480

ccatcccagt ctctgagcgt gacctgtaca gtgtccggcg tgtctctgcc tgactacggc      540

gtgtcctgga tcaggcagcc acctaggaag ggactggagt ggctgggcgt gatctggggc      600

tctgagacca catactataa ttctgccctg aagagccgcc tgaccatcat caaggacaac      660

tccaagtctc aggtgtttct gaagatgaat agcctgcaga ccgacgatac agccatctac      720

tattgcgcca agcactacta ttacggcggc tcctacgcca tggattattg gggccagggc      780

acctccgtga cagtgtctag cgagtctaag tatggcccac cctgccctcc atgtccaatg      840

ttctgggtgc tggtggtggt gggaggcgtg ctggcctgtt actccctgct ggtgaccgtg      900

gcctttatca tcttctgggt gaagagaggc aggaagaagc tgctgtatat ctttaagcag      960

cccttcatgc gccctgtgca gaccacacag gaggaggacg gctgcagctg tcggtttcca     1020

gaggaggagg agggaggatg cgagctgcgc gtgaagttca gccggtccgc cgatgcccct     1080

gcctaccagc agggccagaa ccagctgtat aacgagctga atctgggccg gagagaggag     1140

tacgacgtgc tggataagag gaggggaagg gacccagaga tgggaggcaa gcctcggaga     1200

aagaacccac aggagggcct gtacaatgag ctgcagaagg acaagatggc cgaggcctat     1260

tctgagatcg gcatgaaggg agagaggcgc cggggcaagg gacacgatgg cctgtaccag     1320

ggcctgagca ccgccacaaa ggacacatat gatgccctgc acatgcaggc cctgccacct     1380

aggggatctg gagccaccaa ctttagcctg ctgaagcagg caggcgatgt ggaggagaat     1440

ccaggacctg agatgtggca cgagggactg gaggaggcaa gcaggctgta ctttggcgag     1500

cggaatgtga agggcatgtt cgaggtgctg gagccactgc acgcaatgat ggagagggga     1560

ccacagaccc tgaaggagac atccttcaac caggcatacg gaagggacct gatggaggca     1620

caggagtggt gccggaagta tatgaagtct ggcaatgtga aggacctgct gcaggcctgg     1680

gatctgtatt accacgtgtt tagaaggatc agcaagggct ccggcgccac caacttctcc     1740

ctgctgaagc aggccggcga tgtggaagaa aatccaggac caatgcctct gggactgctg     1800

tggctgggac tggccctgct gggcgccctg cacgcccagg ccggcgtgca ggtggagaca     1860

atcagccctg gcgacggcag aacctttcca aagaggggcc agacatgcgt ggtgcactac     1920

accggcatgc tggaggatgg caagaagttc gactcctctc gcgatcggaa caagcccttt     1980

aagttcatgc tgggcaagca ggaagtgatc agaggctggg aggagggcgt ggcccagatg     2040

tctgtgggcc agagggccaa gctgacaatc agcccagact atgcatacgg agcaaccgga     2100

caccctggaa tcatcccacc acacgccaca ctggtgttcg atgtggagct gctgaagctg     2160

ggcgagggct ctaacaccag caaggagaat ccatttctgt tcgcactgga ggcagtggtc     2220

atctccgtgg gctctatggg cctgatcatc tccctgctgt gcgtgtactt ttggctggag     2280

agaacaatgc caaggatccc caccctgaag aacctggagg acctggtgac cgagtaccac     2340

ggcaatttca gcgcctggtc cggcgtgtct aagggactgg cagagtccct gcagccagat     2400

tattctgagc ggctgtgcct ggtgagcgag atccctccaa agggaggcgc cctgggagag     2460

ggaccaggag ccagcccctg caaccagcac tccccttact gggccccccc ttgttatacc     2520

ctgaagccag agacaggctc tggcgccacc aacttcagcc tgctgaagca agccggcgac     2580

gtggaagaaa acccaggacc aatggcactg ccagtgaccg ccctgctgct gcctctggcc     2640

ctgctgctgc acgcagccag acccatcctg tggcacgaaa tgtggcatga aggcctggag     2700

gaggcaagca gactgtactt tggcgagaga aatgtgaaag gaatgtttga ggtgctggag     2760

cctctgcacg ccatgatgga gaggggccct cagaccctga aggagacatc ctttaaccag     2820

gcctacggca gagacctgat ggaggcccag gagtggtgca ggaagtatat gaagagcgga     2880

aatgtgaaag acctgctgca ggcctgggat ctgtactacc acgtgttccg ccggatctct     2940

aagggcaagg atacaatccc ttggctggga cacctgctgg tgggactgag cggagccttt     3000

ggcttcatca tcctggtgta tctgctgatc aactgcagaa atacaggccc atggctgaag     3060

aaggtgctga agtgtaacac ccctgaccca tccaagttct tttctcagct gagctccgag     3120

cacggcggcg atgtgcagaa gtggctgtct agcccctttc cttcctctag cttcagccct     3180

ggaggactgg cacctgagat ctccccactg gaggtgctgg agagggacaa ggtgacccag     3240

ctgctgctgc agcaggataa ggtgccagag cccgcctccc tgtcctctaa ccacagcctg     3300

acctcctgct ttacaaatca gggctacttc tttttccacc tgccagacgc actggagatc     3360

gaggcatgtc aggtgtattt cacatacgat ccctatagcg aggaggaccc tgatgaggga     3420

gtggccggcg ccccaaccgg aagctcccct cagccactgc agccactgag cggagaggac     3480

gatgcatatt gtacatttcc ttcccgcgac gatctgctgc tgttctctcc aagcctgctg     3540

ggaggaccat ctccacccag caccgcacct ggaggatccg gggcagggga ggagcggatg     3600

cctccatctc tgcaggagag agtgccaagg gactgggatc cacagcctct gggaccacct     3660

acccctggag tgccagacct ggtggatttc cagccacccc ctgagctggt gctgcgggag     3720

gcaggagagg aggtgccaga cgcaggacct agagagggcg tgagctttcc ctggtccagg     3780

ccaccaggac agggagagtt ccgcgccctg aacgcccggc tgcccctgaa tacagacgcc     3840

tacctgtctc tgcaggagct gcagggccag gatcctaccc acctggtgtg a              3891


<210>  62
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding signal peptide (human 
       CSF2r)

<400>  62
atgctgctgc tggtgacctc cctgctgctg tgcgagctgc ctcacccagc ctttctgctg       60

atcccc                                                                  66


<210>  63
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding CD19 scFv (VL-linker-VH)

<400>  63
gacatccaga tgacacagac cacaagctcc ctgtctgcca gcctgggcga cagagtgacc       60

atctcctgta gggcctctca ggatatcagc aagtacctga actggtatca gcagaagcca      120

gatggcacag tgaagctgct gatctaccac acctccaggc tgcactctgg agtgccaagc      180

cggttctccg gatctggaag cggcaccgac tattccctga caatctctaa cctggagcag      240

gaggatatcg ccacatactt ttgccagcag ggcaataccc tgccatatac attcggcgga      300

ggaaccaagc tggagatcac cggatccaca tctggaagcg gcaagccagg aagcggagag      360

ggatccacaa agggagaggt gaagctgcag gagagcggac caggactggt ggcaccatcc      420

cagtctctga gcgtgacctg tacagtgtcc ggcgtgtctc tgcctgacta cggcgtgtcc      480

tggatcaggc agccacctag gaagggactg gagtggctgg gcgtgatctg gggctctgag      540

accacatact ataattctgc cctgaagagc cgcctgacca tcatcaagga caactccaag      600

tctcaggtgt ttctgaagat gaatagcctg cagaccgacg atacagccat ctactattgc      660

gccaagcact actattacgg cggctcctac gccatggatt attggggcca gggcacctcc      720

gtgacagtgt ctagc                                                       735


<210>  64
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding IgG4 linker-CD28 TM part of
       vector construct

<400>  64
gagtctaagt atggcccacc ctgccctcca tgtccaatgt tctgggtgct ggtggtggtg       60

ggaggcgtgc tggcctgtta ctccctgctg gtgaccgtgg cctttatcat cttctgggtg      120


<210>  65
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding "4-1BB signal domain part 
       of vector construct

<400>  65
aagagaggca ggaagaagct gctgtatatc tttaagcagc ccttcatgcg ccctgtgcag       60

accacacagg aggaggacgg ctgcagctgt cggtttccag aggaggagga gggaggatgc      120

gagctg                                                                 126


<210>  66
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding CD3zeta part of vector 
       construct

<400>  66
cgcgtgaagt tcagccggtc cgccgatgcc cctgcctacc agcagggcca gaaccagctg       60

tataacgagc tgaatctggg ccggagagag gagtacgacg tgctggataa gaggagggga      120

agggacccag agatgggagg caagcctcgg agaaagaacc cacaggaggg cctgtacaat      180

gagctgcaga aggacaagat ggccgaggcc tattctgaga tcggcatgaa gggagagagg      240

cgccggggca agggacacga tggcctgtac cagggcctga gcaccgccac aaaggacaca      300

tatgatgccc tgcacatgca ggccctgcca cctagg                                336


<210>  67
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucloetide encoding P@A self-cleaving peptide 
       part of vector construct

<400>  67
ggatctggag ccaccaactt tagcctgctg aagcaggcag gcgatgtgga ggagaatcca       60

ggacct                                                                  66


<210>  68
<211>  267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide enconding Naked FRB part of vector 
       construct

<400>  68
gagatgtggc acgagggact ggaggaggca agcaggctgt actttggcga gcggaatgtg       60

aagggcatgt tcgaggtgct ggagccactg cacgcaatga tggagagggg accacagacc      120

ctgaaggaga catccttcaa ccaggcatac ggaagggacc tgatggaggc acaggagtgg      180

tgccggaagt atatgaagtc tggcaatgtg aaggacctgc tgcaggcctg ggatctgtat      240

taccacgtgt ttagaaggat cagcaag                                          267


<210>  69
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding P2A self-cleaving peptide 
       part of vector construct

<400>  69
ggctccggcg ccaccaactt ctccctgctg aagcaggccg gcgatgtgga agaaaatcca       60

ggacca                                                                  66


<210>  70
<211>  378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding signal peptide FKBP12 part 
       of vector construct

<400>  70
atgcctctgg gactgctgtg gctgggactg gccctgctgg gcgccctgca cgcccaggcc       60

ggcgtgcagg tggagacaat cagccctggc gacggcagaa cctttccaaa gaggggccag      120

acatgcgtgg tgcactacac cggcatgctg gaggatggca agaagttcga ctcctctcgc      180

gatcggaaca agccctttaa gttcatgctg ggcaagcagg aagtgatcag aggctgggag      240

gagggcgtgg cccagatgtc tgtgggccag agggccaagc tgacaatcag cccagactat      300

gcatacggag caaccggaca ccctggaatc atcccaccac acgccacact ggtgttcgat      360

gtggagctgc tgaagctg                                                    378


<210>  71
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding "IL-2R gamma TM-Cytoplasmic
       domain part of vector construct

<400>  71
ggcgagggct ctaacaccag caaggagaat ccatttctgt tcgcactgga ggcagtggtc       60

atctccgtgg gctctatggg cctgatcatc tccctgctgt gcgtgtactt ttggctggag      120

agaacaatgc caaggatccc caccctgaag aacctggagg acctggtgac cgagtaccac      180

ggcaatttca gcgcctggtc cggcgtgtct aagggactgg cagagtccct gcagccagat      240

tattctgagc ggctgtgcct ggtgagcgag atccctccaa agggaggcgc cctgggagag      300

ggaccaggag ccagcccctg caaccagcac tccccttact gggccccccc ttgttatacc      360

ctgaagccag agaca                                                       375


<210>  72
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding P2A self-cleaving peptide 
       part of vector construct

<400>  72
ggctctggcg ccaccaactt cagcctgctg aagcaagccg gcgacgtgga agaaaaccca       60

ggacca                                                                  66


<210>  73
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding signal peptideFRB part of 
       vector construct

<400>  73
atggcactgc cagtgaccgc cctgctgctg cctctggccc tgctgctgca cgcagccaga       60

cccatcctgt ggcacgaaat gtggcatgaa ggcctggagg aggcaagcag actgtacttt      120

ggcgagagaa atgtgaaagg aatgtttgag gtgctggagc ctctgcacgc catgatggag      180

aggggccctc agaccctgaa ggagacatcc tttaaccagg cctacggcag agacctgatg      240

gaggcccagg agtggtgcag gaagtatatg aagagcggaa atgtgaaaga cctgctgcag      300

gcctgggatc tgtactacca cgtgttccgc cggatctcta ag                         342


<210>  74
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding "IL-2R beta TM-Cytoplasmic 
       domain part of vector construct

<400>  74
ggcaaggata caatcccttg gctgggacac ctgctggtgg gactgagcgg agcctttggc       60

ttcatcatcc tggtgtatct gctgatcaac tgcagaaata caggcccatg gctgaagaag      120

gtgctgaagt gtaacacccc tgacccatcc aagttctttt ctcagctgag ctccgagcac      180

ggcggcgatg tgcagaagtg gctgtctagc ccctttcctt cctctagctt cagccctgga      240

ggactggcac ctgagatctc cccactggag gtgctggaga gggacaaggt gacccagctg      300

ctgctgcagc aggataaggt gccagagccc gcctccctgt cctctaacca cagcctgacc      360

tcctgcttta caaatcaggg ctacttcttt ttccacctgc cagacgcact ggagatcgag      420

gcatgtcagg tgtatttcac atacgatccc tatagcgagg aggaccctga tgagggagtg      480

gccggcgccc caaccggaag ctcccctcag ccactgcagc cactgagcgg agaggacgat      540

gcatattgta catttccttc ccgcgacgat ctgctgctgt tctctccaag cctgctggga      600

ggaccatctc cacccagcac cgcacctgga ggatccgggg caggggagga gcggatgcct      660

ccatctctgc aggagagagt gccaagggac tgggatccac agcctctggg accacctacc      720

cctggagtgc cagacctggt ggatttccag ccaccccctg agctggtgct gcgggaggca      780

ggagaggagg tgccagacgc aggacctaga gagggcgtga gctttccctg gtccaggcca      840

ccaggacagg gagagttccg cgccctgaac gcccggctgc ccctgaatac agacgcctac      900

ctgtctctgc aggagctgca gggccaggat cctacccacc tggtg                      945


<210>  75
<211>  1297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB_RACR_CD19 CAR lentiviral vector Construct

<400>  75

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly Ser Gly Ala Thr Asn 
                85                  90                  95      


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            100                 105                 110         


Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
        115                 120                 125             


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
    130                 135                 140                 


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
145                 150                 155                 160 


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
                165                 170                 175     


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
            180                 185                 190         


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
        195                 200                 205             


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
    210                 215                 220                 


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Gly Glu 
225                 230                 235                 240 


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
                245                 250                 255     


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
            260                 265                 270         


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
        275                 280                 285             


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
    290                 295                 300                 


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
305                 310                 315                 320 


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
                325                 330                 335     


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
            340                 345                 350         


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr Gly Ser Gly Ala Thr 
        355                 360                 365             


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
    370                 375                 380                 


Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu 
385                 390                 395                 400 


Leu His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly 
                405                 410                 415     


Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly 
            420                 425                 430         


Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro 
        435                 440                 445             


Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu 
    450                 455                 460                 


Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val 
465                 470                 475                 480 


Lys Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg 
                485                 490                 495     


Ile Ser Lys Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val 
            500                 505                 510         


Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile 
        515                 520                 525             


Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
    530                 535                 540                 


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
545                 550                 555                 560 


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
                565                 570                 575     


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
            580                 585                 590         


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
        595                 600                 605             


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
    610                 615                 620                 


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
625                 630                 635                 640 


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
                645                 650                 655     


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
            660                 665                 670         


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
        675                 680                 685             


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
    690                 695                 700                 


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
705                 710                 715                 720 


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
                725                 730                 735     


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
            740                 745                 750         


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
        755                 760                 765             


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
    770                 775                 780                 


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
785                 790                 795                 800 


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val Gly Ser 
                805                 810                 815     


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
            820                 825                 830         


Asn Pro Gly Pro Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu 
        835                 840                 845             


Leu Pro His Pro Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln 
    850                 855                 860                 


Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser 
865                 870                 875                 880 


Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln 
                885                 890                 895     


Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu 
            900                 905                 910         


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        915                 920                 925             


Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr 
    930                 935                 940                 


Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr 
945                 950                 955                 960 


Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser 
                965                 970                 975     


Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro 
            980                 985                 990         


Gly Leu Val Ala Pro Ser Gln Ser  Leu Ser Val Thr Cys  Thr Val Ser 
        995                 1000                 1005             


Gly Val  Ser Leu Pro Asp Tyr  Gly Val Ser Trp Ile  Arg Gln Pro 
    1010                 1015                 1020             


Pro Arg  Lys Gly Leu Glu Trp  Leu Gly Val Ile Trp  Gly Ser Glu 
    1025                 1030                 1035             


Thr Thr  Tyr Tyr Asn Ser Ala  Leu Lys Ser Arg Leu  Thr Ile Ile 
    1040                 1045                 1050             


Lys Asp  Asn Ser Lys Ser Gln  Val Phe Leu Lys Met  Asn Ser Leu 
    1055                 1060                 1065             


Gln Thr  Asp Asp Thr Ala Ile  Tyr Tyr Cys Ala Lys  His Tyr Tyr 
    1070                 1075                 1080             


Tyr Gly  Gly Ser Tyr Ala Met  Asp Tyr Trp Gly Gln  Gly Thr Ser 
    1085                 1090                 1095             


Val Thr  Val Ser Ser Glu Ser  Lys Tyr Gly Pro Pro  Cys Pro Pro 
    1100                 1105                 1110             


Cys Pro  Met Phe Trp Val Leu  Val Val Val Gly Gly  Val Leu Ala 
    1115                 1120                 1125             


Cys Tyr  Ser Leu Leu Val Thr  Val Ala Phe Ile Ile  Phe Trp Val 
    1130                 1135                 1140             


Lys Arg  Gly Arg Lys Lys Leu  Leu Tyr Ile Phe Lys  Gln Pro Phe 
    1145                 1150                 1155             


Met Arg  Pro Val Gln Thr Thr  Gln Glu Glu Asp Gly  Cys Ser Cys 
    1160                 1165                 1170             


Arg Phe  Pro Glu Glu Glu Glu  Gly Gly Cys Glu Leu  Arg Val Lys 
    1175                 1180                 1185             


Phe Ser  Arg Ser Ala Asp Ala  Pro Ala Tyr Gln Gln  Gly Gln Asn 
    1190                 1195                 1200             


Gln Leu  Tyr Asn Glu Leu Asn  Leu Gly Arg Arg Glu  Glu Tyr Asp 
    1205                 1210                 1215             


Val Leu  Asp Lys Arg Arg Gly  Arg Asp Pro Glu Met  Gly Gly Lys 
    1220                 1225                 1230             


Pro Arg  Arg Lys Asn Pro Gln  Glu Gly Leu Tyr Asn  Glu Leu Gln 
    1235                 1240                 1245             


Lys Asp  Lys Met Ala Glu Ala  Tyr Ser Glu Ile Gly  Met Lys Gly 
    1250                 1255                 1260             


Glu Arg  Arg Arg Gly Lys Gly  His Asp Gly Leu Tyr  Gln Gly Leu 
    1265                 1270                 1275             


Ser Thr  Ala Thr Lys Asp Thr  Tyr Asp Ala Leu His  Met Gln Ala 
    1280                 1285                 1290             


Leu Pro  Pro Arg 
    1295         


<210>  76
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Naked FRB_P2A part of construct

<400>  76

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly Ser Gly Ala Thr Asn 
                85                  90                  95      


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            100                 105                 110         


<210>  77
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - RACRg (FKBP12_IL-2R gamma)_P2A part of construct

<400>  77

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Gly Glu 
        115                 120                 125             


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
    130                 135                 140                 


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
145                 150                 155                 160 


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
                165                 170                 175     


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
            180                 185                 190         


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
        195                 200                 205             


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
    210                 215                 220                 


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
225                 230                 235                 240 


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr Gly Ser Gly Ala Thr 
                245                 250                 255     


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
            260                 265                 270         


Pro 
    


<210>  78
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - RACRb (FRB_IL-2R beta)_P2A part of construct

<400>  78

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly 
        115                 120                 125             


Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn 
    130                 135                 140                 


Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr 
145                 150                 155                 160 


Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly 
                165                 170                 175     


Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser 
            180                 185                 190         


Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg 
        195                 200                 205             


Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro 
    210                 215                 220                 


Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln 
225                 230                 235                 240 


Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys 
                245                 250                 255     


Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu 
            260                 265                 270         


Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro 
        275                 280                 285             


Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp 
    290                 295                 300                 


Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser 
305                 310                 315                 320 


Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser 
                325                 330                 335     


Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro 
            340                 345                 350         


Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu 
        355                 360                 365             


Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg 
    370                 375                 380                 


Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe 
385                 390                 395                 400 


Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser 
                405                 410                 415     


Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val Gly Ser Gly 
            420                 425                 430         


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
        435                 440                 445             


Pro Gly Pro 
    450     


<210>  79
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - CD19 scFv part of construct

<400>  79

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265         


<210>  80
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IgG4 linker-CD28 TM_4-1BB-CD3zeta part of construct

<400>  80

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val 
1               5                   10                  15      


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
            20                  25                  30          


Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu 
        35                  40                  45              


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
    50                  55                  60                  


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
65                  70                  75                  80  


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                85                  90                  95      


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            100                 105                 110         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        115                 120                 125             


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    130                 135                 140                 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
145                 150                 155                 160 


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                165                 170                 175     


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            180                 185                 190         


Pro Arg 
        


<210>  81
<211>  3894
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding FRB_RACR_CD19 CAR 
       lentiviral vector construct

<400>  81
atggagatgt ggcacgaggg actggaggag gcaagcagac tgtactttgg cgagaggaac       60

gtgaagggca tgttcgaggt gctggagcca ctgcacgcaa tgatggagag gggaccacag      120

accctgaagg agacatcttt caaccaggca tacggaaggg acctgatgga ggcacaggag      180

tggtgccgga agtatatgaa gagcggcaat gtgaaggacc tgctgcaggc ctgggatctg      240

tactatcacg tgtttcggag aatctccaag ggctctggcg ccaccaactt ctccctgctg      300

aagcaggccg gcgatgtgga ggagaatcct ggaccaatgc cactgggact gctgtggctg      360

ggactggccc tgctgggcgc cctgcacgcc caggccggcg tgcaggtgga gacaatcagc      420

cctggcgacg gacgcacctt tccaaagagg ggacagacat gcgtggtgca ctacaccggc      480

atgctggagg atggcaagaa gttcgacagc tccagagata ggaataagcc ctttaagttc      540

atgctgggca agcaggaagt gatcagggga tgggaggagg gagtggcaca gatgtctgtg      600

ggacagcggg ccaagctgac aatcagccca gactatgcat acggagcaac cggacaccct      660

ggaatcatcc cacctcacgc cacactggtg tttgatgtgg agctgctgaa gctgggcgag      720

ggcagcaaca cctccaagga gaatccattt ctgttcgccc tggaggccgt ggtcatctct      780

gtgggcagca tgggcctgat catctccctg ctgtgcgtgt acttttggct ggagcgcaca      840

atgccacgga tccccaccct gaagaacctg gaggacctgg tgaccgagta ccacggcaat      900

ttctccgcct ggtctggcgt gagcaaggga ctggcagagt ctctgcagcc agattatagc      960

gagcggctgt gcctggtgag cgagatccca cccaagggag gcgccctggg agagggacca     1020

ggagcctccc cttgcaacca gcactctcct tactgggccc ctccatgtta taccctgaag     1080

ccagagacag gcagcggagc tactaacttc tccctgctga agcaagcagg cgacgtggaa     1140

gaaaatcctg gaccaatggc actgccagtg accgccctgc tgctgcctct ggccctgctg     1200

ctgcacgcag ccagacccat cctgtggcac gaaatgtggc atgaaggcct ggaggaggca     1260

agcaggctgt actttggcga gcggaatgtg aaaggaatgt ttgaagtgct ggagcctctg     1320

cacgccatga tggagagggg ccctcagacc ctgaaggaga catcctttaa ccaggcctac     1380

ggcagagacc tgatggaggc ccaggagtgg tgcaggaagt atatgaagtc tggaaatgtg     1440

aaagacctgc tgcaggcctg ggatctgtat tatcacgtgt tcaggcgcat ctctaagggc     1500

aaggatacaa tcccttggct gggacacctg ctggtgggac tgagcggagc ctttggcttc     1560

atcatcctgg tgtatctgct gatcaactgc cgcaatacag gcccatggct gaagaaggtg     1620

ctgaagtgta acacccccga cccttccaag ttcttttctc agctgtctag cgagcacggc     1680

ggcgatgtgc agaagtggct gtcctctcca tttcccagct cctctttcag cccaggagga     1740

ctggcaccag agatctcccc actggaggtg ctggagaggg acaaggtgac ccagctgctg     1800

ctgcagcagg ataaggtgcc tgagccagcc tccctgagct ccaaccactc cctgacctct     1860

tgctttacaa atcagggcta cttctttttc cacctgccag acgcactgga gatcgaggca     1920

tgtcaggtgt atttcacata cgatccctat agcgaggagg accctgatga gggagtggcc     1980

ggcgccccaa ccggatctag cccacagcct ctgcagccac tgagcggaga ggacgatgca     2040

tattgtacat ttccttcccg cgacgatctg ctgctgttct ctccaagcct gctgggagga     2100

ccaagcccac cttccaccgc accaggcggc tccggggcag gggaggagcg gatgccaccc     2160

tctctgcagg agagagtgcc aagggactgg gatccacagc cactgggacc tccaacccct     2220

ggagtgccag acctggtgga tttccagccc cctccagagc tggtgctgag agaggcagga     2280

gaggaggtgc ctgacgcagg accaagagag ggcgtgagct ttccttggtc caggccacct     2340

ggacagggag agttcagagc cctgaacgcc aggctgcccc tgaatacaga cgcctacctg     2400

tctctgcagg agctgcaggg ccaggatcct acacacctgg tcggatctgg cgccaccaac     2460

tttagcctgc tgaagcaggc aggcgacgtg gaagagaacc ctggaccaat gctgctgctg     2520

gtgaccagcc tgctgctgtg cgagctgcca caccctgcct tcctgctgat cccagatatc     2580

cagatgacac agaccacatc ctctctgtcc gcctctctgg gcgacagagt gaccatctct     2640

tgtagggcca gccaggatat ctccaagtac ctgaactggt atcagcagaa gcctgacggc     2700

acagtgaagc tgctgatcta ccacacctct aggctgcaca gcggagtgcc atcccggttc     2760

agcggatccg gatctggaac agactattct ctgaccatca gcaacctgga gcaggaggat     2820

atcgccacat acttttgcca gcagggcaat accctgccat atacattcgg cggaggaacc     2880

aagctggaga tcaccggaag cacatccgga tctggcaagc caggatccgg agagggatct     2940

acaaagggag aggtgaagct gcaggagagc ggaccaggac tggtggcacc cagccagtcc     3000

ctgtctgtga cctgtacagt gtctggcgtg agcctgcccg attacggcgt gtcctggatc     3060

agacagccac caaggaaggg actggagtgg ctgggcgtga tctggggctc tgagaccaca     3120

tactataata gcgccctgaa gtcccggctg accatcatca aggacaacag caagtcccag     3180

gtgtttctga agatgaatag cctgcagacc gacgatacag ccatctacta ttgcgccaag     3240

cactactatt acggcggctc ctacgccatg gattattggg gccagggcac ctccgtgaca     3300

gtgagctccg agtctaagta tggccctcca tgcccccctt gtcctatgtt ctgggtgctg     3360

gtggtggtgg gaggcgtgct ggcctgttac tccctgctgg tgaccgtggc ctttatcatc     3420

ttctgggtga agcgcggccg gaagaagctg ctgtatatct ttaagcagcc cttcatgaga     3480

cctgtgcaga ccacacagga ggaggacggc tgcagctgta ggtttccaga ggaggaggag     3540

ggaggatgcg agctgcgcgt gaagttctct cggagcgccg atgcccctgc ctaccagcag     3600

ggacagaacc agctgtataa cgagctgaat ctgggccgga gagaggagta cgacgtgctg     3660

gataagagga ggggaagaga cccagagatg ggaggcaagc ctcggagaaa gaacccacag     3720

gagggcctgt acaatgagct gcagaaggac aagatggccg aggcctattc cgagatcggc     3780

atgaagggag agaggcgccg gggcaaggga cacgatggcc tgtaccaggg cctgagcacc     3840

gccacaaagg acacctatga tgccctgcac atgcaggccc tgccacccag gtga           3894


<210>  82
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding Naked FRB_P2A part of 
       vector construct

<400>  82
atggagatgt ggcacgaggg actggaggag gcaagcagac tgtactttgg cgagaggaac       60

gtgaagggca tgttcgaggt gctggagcca ctgcacgcaa tgatggagag gggaccacag      120

accctgaagg agacatcttt caaccaggca tacggaaggg acctgatgga ggcacaggag      180

tggtgccgga agtatatgaa gagcggcaat gtgaaggacc tgctgcaggc ctgggatctg      240

tactatcacg tgtttcggag aatctccaag ggctctggcg ccaccaactt ctccctgctg      300

aagcaggccg gcgatgtgga ggagaatcct ggacca                                336


<210>  83
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding RACRg (FKBP12_IL-2R 
       gamma)_P2A part of vector construct

<400>  83
atgccactgg gactgctgtg gctgggactg gccctgctgg gcgccctgca cgcccaggcc       60

ggcgtgcagg tggagacaat cagccctggc gacggacgca cctttccaaa gaggggacag      120

acatgcgtgg tgcactacac cggcatgctg gaggatggca agaagttcga cagctccaga      180

gataggaata agccctttaa gttcatgctg ggcaagcagg aagtgatcag gggatgggag      240

gagggagtgg cacagatgtc tgtgggacag cgggccaagc tgacaatcag cccagactat      300

gcatacggag caaccggaca ccctggaatc atcccacctc acgccacact ggtgtttgat      360

gtggagctgc tgaagctggg cgagggcagc aacacctcca aggagaatcc atttctgttc      420

gccctggagg ccgtggtcat ctctgtgggc agcatgggcc tgatcatctc cctgctgtgc      480

gtgtactttt ggctggagcg cacaatgcca cggatcccca ccctgaagaa cctggaggac      540

ctggtgaccg agtaccacgg caatttctcc gcctggtctg gcgtgagcaa gggactggca      600

gagtctctgc agccagatta tagcgagcgg ctgtgcctgg tgagcgagat cccacccaag      660

ggaggcgccc tgggagaggg accaggagcc tccccttgca accagcactc tccttactgg      720

gcccctccat gttataccct gaagccagag acaggcagcg gagctactaa cttctccctg      780

ctgaagcaag caggcgacgt ggaagaaaat cctggacca                             819


<210>  84
<211>  1353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding RACRb (FRB_IL-2R beta)_P2A 
       part of vector construct

<400>  84
atggcactgc cagtgaccgc cctgctgctg cctctggccc tgctgctgca cgcagccaga       60

cccatcctgt ggcacgaaat gtggcatgaa ggcctggagg aggcaagcag gctgtacttt      120

ggcgagcgga atgtgaaagg aatgtttgaa gtgctggagc ctctgcacgc catgatggag      180

aggggccctc agaccctgaa ggagacatcc tttaaccagg cctacggcag agacctgatg      240

gaggcccagg agtggtgcag gaagtatatg aagtctggaa atgtgaaaga cctgctgcag      300

gcctgggatc tgtattatca cgtgttcagg cgcatctcta agggcaagga tacaatccct      360

tggctgggac acctgctggt gggactgagc ggagcctttg gcttcatcat cctggtgtat      420

ctgctgatca actgccgcaa tacaggccca tggctgaaga aggtgctgaa gtgtaacacc      480

cccgaccctt ccaagttctt ttctcagctg tctagcgagc acggcggcga tgtgcagaag      540

tggctgtcct ctccatttcc cagctcctct ttcagcccag gaggactggc accagagatc      600

tccccactgg aggtgctgga gagggacaag gtgacccagc tgctgctgca gcaggataag      660

gtgcctgagc cagcctccct gagctccaac cactccctga cctcttgctt tacaaatcag      720

ggctacttct ttttccacct gccagacgca ctggagatcg aggcatgtca ggtgtatttc      780

acatacgatc cctatagcga ggaggaccct gatgagggag tggccggcgc cccaaccgga      840

tctagcccac agcctctgca gccactgagc ggagaggacg atgcatattg tacatttcct      900

tcccgcgacg atctgctgct gttctctcca agcctgctgg gaggaccaag cccaccttcc      960

accgcaccag gcggctccgg ggcaggggag gagcggatgc caccctctct gcaggagaga     1020

gtgccaaggg actgggatcc acagccactg ggacctccaa cccctggagt gccagacctg     1080

gtggatttcc agccccctcc agagctggtg ctgagagagg caggagagga ggtgcctgac     1140

gcaggaccaa gagagggcgt gagctttcct tggtccaggc cacctggaca gggagagttc     1200

agagccctga acgccaggct gcccctgaat acagacgcct acctgtctct gcaggagctg     1260

cagggccagg atcctacaca cctggtcgga tctggcgcca ccaactttag cctgctgaag     1320

caggcaggcg acgtggaaga gaaccctgga cca                                  1353


<210>  85
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding CD19 scFv part of vector 
       construct

<400>  85
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cacaccctgc cttcctgctg       60

atcccagata tccagatgac acagaccaca tcctctctgt ccgcctctct gggcgacaga      120

gtgaccatct cttgtagggc cagccaggat atctccaagt acctgaactg gtatcagcag      180

aagcctgacg gcacagtgaa gctgctgatc taccacacct ctaggctgca cagcggagtg      240

ccatcccggt tcagcggatc cggatctgga acagactatt ctctgaccat cagcaacctg      300

gagcaggagg atatcgccac atacttttgc cagcagggca ataccctgcc atatacattc      360

ggcggaggaa ccaagctgga gatcaccgga agcacatccg gatctggcaa gccaggatcc      420

ggagagggat ctacaaaggg agaggtgaag ctgcaggaga gcggaccagg actggtggca      480

cccagccagt ccctgtctgt gacctgtaca gtgtctggcg tgagcctgcc cgattacggc      540

gtgtcctgga tcagacagcc accaaggaag ggactggagt ggctgggcgt gatctggggc      600

tctgagacca catactataa tagcgccctg aagtcccggc tgaccatcat caaggacaac      660

agcaagtccc aggtgtttct gaagatgaat agcctgcaga ccgacgatac agccatctac      720

tattgcgcca agcactacta ttacggcggc tcctacgcca tggattattg gggccagggc      780

acctccgtga cagtgagctc c                                                801


<210>  86
<211>  582
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding IgG4 linker-CD28 
       TM_4-1BB-CD3zeta part of vector construct

<400>  86
gagtctaagt atggccctcc atgcccccct tgtcctatgt tctgggtgct ggtggtggtg       60

ggaggcgtgc tggcctgtta ctccctgctg gtgaccgtgg cctttatcat cttctgggtg      120

aagcgcggcc ggaagaagct gctgtatatc tttaagcagc ccttcatgag acctgtgcag      180

accacacagg aggaggacgg ctgcagctgt aggtttccag aggaggagga gggaggatgc      240

gagctgcgcg tgaagttctc tcggagcgcc gatgcccctg cctaccagca gggacagaac      300

cagctgtata acgagctgaa tctgggccgg agagaggagt acgacgtgct ggataagagg      360

aggggaagag acccagagat gggaggcaag cctcggagaa agaacccaca ggagggcctg      420

tacaatgagc tgcagaagga caagatggcc gaggcctatt ccgagatcgg catgaaggga      480

gagaggcgcc ggggcaaggg acacgatggc ctgtaccagg gcctgagcac cgccacaaag      540

gacacctatg atgccctgca catgcaggcc ctgccaccca gg                         582


<210>  87
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB_CD19 CAR_TGFbDN lentiviral vector Construct

<400>  87

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly Ser Gly Ala Thr Asn 
                85                  90                  95      


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            100                 105                 110         


Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
        115                 120                 125             


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
145                 150                 155                 160 


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
                165                 170                 175     


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
            180                 185                 190         


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
        195                 200                 205             


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
    210                 215                 220                 


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
                245                 250                 255     


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
            260                 265                 270         


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
        275                 280                 285             


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
    290                 295                 300                 


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
305                 310                 315                 320 


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
                325                 330                 335     


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
            340                 345                 350         


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
        355                 360                 365             


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly 
    370                 375                 380                 


Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly 
385                 390                 395                 400 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                405                 410                 415     


Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            420                 425                 430         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        435                 440                 445             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    450                 455                 460                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
465                 470                 475                 480 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                485                 490                 495     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            500                 505                 510         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        515                 520                 525             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    530                 535                 540                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
545                 550                 555                 560 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
                565                 570                 575     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            580                 585                 590         


Pro Gly Pro Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His 
        595                 600                 605             


Ile Val Leu Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln 
    610                 615                 620                 


Lys Ser Val Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val 
625                 630                 635                 640 


Lys Phe Pro Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys 
                645                 650                 655     


Asp Asn Gln Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys 
            660                 665                 670         


Glu Lys Pro Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu 
        675                 680                 685             


Asn Ile Thr Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His 
    690                 695                 700                 


Asp Phe Ile Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu 
705                 710                 715                 720 


Lys Lys Lys Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp 
                725                 730                 735     


Glu Cys Asn Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn 
            740                 745                 750         


Pro Asp Leu Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu 
        755                 760                 765             


Pro Pro Leu Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr 
    770                 775                 780                 


Arg Val Asn Arg Gln Gln Lys Arg Arg Arg 
785                 790                 


<210>  88
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Naked FRB_P2A part of construct

<400>  88

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Gly Ser Gly Ala Thr Asn 
                85                  90                  95      


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            100                 105                 110         


<210>  89
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - CD19 scFv part of construct

<400>  89

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265         


<210>  90
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IgG4 linker-CD28 TM_4-1BB-CD3zeta_P2A part of 
       construct

<400>  90

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val 
1               5                   10                  15      


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
            20                  25                  30          


Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu 
        35                  40                  45              


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
    50                  55                  60                  


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
65                  70                  75                  80  


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                85                  90                  95      


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            100                 105                 110         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        115                 120                 125             


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    130                 135                 140                 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
145                 150                 155                 160 


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                165                 170                 175     


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            180                 185                 190         


Pro Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
        195                 200                 205             


Asp Val Glu Glu Asn Pro Gly Pro 
    210                 215     


<210>  91
<211>  199
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - TGF beta DN part of construct

<400>  91

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Val Asn 
            180                 185                 190         


Arg Gln Gln Lys Arg Arg Arg 
        195                 


<210>  92
<211>  2385
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding FRB_CD19 CAR_TGFbDN 
       lentiviral vector construct

<400>  92
atggagatgt ggcacgaggg actggaggag gcatccagac tgtacttcgg cgagaggaac       60

gtgaagggca tgtttgaggt gctggagcca ctgcacgcca tgatggagag aggcccccag      120

accctgaagg agacatcttt caaccaggcc tatggaaggg acctgatgga ggcacaggag      180

tggtgccgga agtacatgaa gagcggcaat gtgaaggacc tgctgcaggc ctgggatctg      240

tactatcacg tgttccggag aatcagcaag ggctccggcg ccaccaactt tagcctgctg      300

aagcaggcag gcgacgtgga ggagaatcca ggacctatgc tgctgctggt gacatccctg      360

ctgctgtgcg agctgccaca cccagccttc ctgctgatcc ccgatatcca gatgacccag      420

accacaagct ccctgagcgc ctccctgggc gacagggtga caatctcttg tcgggccagc      480

caggatatct ccaagtatct gaattggtac cagcagaagc ccgacggcac cgtgaagctg      540

ctgatctatc acacatctag actgcacagc ggcgtgcctt ccaggttttc tggcagcggc      600

tccggcaccg actactctct gacaatcagc aacctggagc aggaggatat cgccacctat      660

ttctgccagc agggcaatac cctgccttac acatttggcg gcggcacaaa gctggagatc      720

accggctcta caagcggatc cggcaagcca ggatccggag agggatctac caagggagag      780

gtgaagctgc aggagagcgg acctggactg gtggcaccat ctcagagcct gtccgtgacc      840

tgtacagtgt ctggcgtgag cctgccagat tatggcgtga gctggatcag gcagccacct      900

aggaagggac tggagtggct gggcgtgatc tggggctccg agaccacata ctataacagc      960

gccctgaagt cccgcctgac catcatcaag gacaactcta agagccaggt gttcctgaag     1020

atgaattccc tgcagaccga cgatacagcc atctactatt gcgccaagca ctactattac     1080

ggcggctctt atgccatgga ttactggggc cagggcacca gcgtgacagt gtctagcgag     1140

tccaagtacg gcccaccctg ccctccatgt cccatgtttt gggtgctggt ggtggtggga     1200

ggcgtgctgg cctgttattc cctgctggtg accgtggcct tcatcatctt ttgggtgaag     1260

cgcggccgga agaagctgct gtacatcttc aagcagccct tcatgagacc cgtgcagacc     1320

acacaggagg aggacggctg cagctgtagg ttcccagagg aggaggaggg aggatgcgag     1380

ctgagggtga agttttcccg gtctgccgat gcccctgcct atcagcaggg ccagaatcag     1440

ctgtacaacg agctgaatct gggcaggcgc gaggagtacg acgtgctgga taagaggaga     1500

ggaagggacc ctgagatggg aggcaagcca aggcgcaaga accctcagga gggcctgtat     1560

aatgagctgc agaaggacaa gatggccgag gcctactccg agatcggcat gaagggagag     1620

cggagaaggg gcaagggaca cgatggcctg tatcagggcc tgagcaccgc cacaaaggac     1680

acctacgatg cactgcacat gcaggccctg ccacctagag gatctggagc cacaaacttc     1740

agcctgctga agcaggccgg cgatgtggag gagaatcctg gaccaatggg aagaggactg     1800

ctgaggggac tgtggccact gcacatcgtg ctgtggacca ggatcgcctc tacaatccca     1860

ccccacgtgc agaagagcgt gaacaatgac atgatcgtga ccgataacaa tggcgccgtg     1920

aagtttcccc agctgtgcaa gttctgtgac gtgcgctttt ccacctgtga taaccagaag     1980

tcctgcatgt ctaattgtag catcacatcc atctgcgaga agcctcagga ggtgtgcgtg     2040

gccgtgtggc ggaagaacga cgagaatatc accctggaga cagtgtgcca cgatcccaag     2100

ctgccttatc acgacttcat cctggaggat gccgcctctc ctaagtgtat catgaaggag     2160

aagaagaagc caggcgagac cttctttatg tgcagctgtt cctctgacga gtgcaacgat     2220

aatatcatct tctccgagga gtacaacacc tctaatcctg acctgctgct ggtcatcttt     2280

caggtgacag gcatctccct gctgcctcca ctgggcgtgg ccatctctgt gatcatcatc     2340

ttttattgtt acagagtgaa caggcagcag aagcgccggc gctag                     2385


<210>  93
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding Naked FRB_P2A part of 
       vector construct

<400>  93
atggagatgt ggcacgaggg actggaggag gcatccagac tgtacttcgg cgagaggaac       60

gtgaagggca tgtttgaggt gctggagcca ctgcacgcca tgatggagag aggcccccag      120

accctgaagg agacatcttt caaccaggcc tatggaaggg acctgatgga ggcacaggag      180

tggtgccgga agtacatgaa gagcggcaat gtgaaggacc tgctgcaggc ctgggatctg      240

tactatcacg tgttccggag aatcagcaag ggctccggcg ccaccaactt tagcctgctg      300

aagcaggcag gcgacgtgga ggagaatcca ggacct                                336


<210>  94
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding CD19 scFv part of vector 
       construct

<400>  94
atgctgctgc tggtgacatc cctgctgctg tgcgagctgc cacacccagc cttcctgctg       60

atccccgata tccagatgac ccagaccaca agctccctga gcgcctccct gggcgacagg      120

gtgacaatct cttgtcgggc cagccaggat atctccaagt atctgaattg gtaccagcag      180

aagcccgacg gcaccgtgaa gctgctgatc tatcacacat ctagactgca cagcggcgtg      240

ccttccaggt tttctggcag cggctccggc accgactact ctctgacaat cagcaacctg      300

gagcaggagg atatcgccac ctatttctgc cagcagggca ataccctgcc ttacacattt      360

ggcggcggca caaagctgga gatcaccggc tctacaagcg gatccggcaa gccaggatcc      420

ggagagggat ctaccaaggg agaggtgaag ctgcaggaga gcggacctgg actggtggca      480

ccatctcaga gcctgtccgt gacctgtaca gtgtctggcg tgagcctgcc agattatggc      540

gtgagctgga tcaggcagcc acctaggaag ggactggagt ggctgggcgt gatctggggc      600

tccgagacca catactataa cagcgccctg aagtcccgcc tgaccatcat caaggacaac      660

tctaagagcc aggtgttcct gaagatgaat tccctgcaga ccgacgatac agccatctac      720

tattgcgcca agcactacta ttacggcggc tcttatgcca tggattactg gggccagggc      780

accagcgtga cagtgtctag c                                                801


<210>  95
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding IgG4 linker-CD28 
       TM_4-1BB-CD3zeta_P2A part of vector construct

<400>  95
gagtccaagt acggcccacc ctgccctcca tgtcccatgt tttgggtgct ggtggtggtg       60

ggaggcgtgc tggcctgtta ttccctgctg gtgaccgtgg ccttcatcat cttttgggtg      120

aagcgcggcc ggaagaagct gctgtacatc ttcaagcagc ccttcatgag acccgtgcag      180

accacacagg aggaggacgg ctgcagctgt aggttcccag aggaggagga gggaggatgc      240

gagctgaggg tgaagttttc ccggtctgcc gatgcccctg cctatcagca gggccagaat      300

cagctgtaca acgagctgaa tctgggcagg cgcgaggagt acgacgtgct ggataagagg      360

agaggaaggg accctgagat gggaggcaag ccaaggcgca agaaccctca ggagggcctg      420

tataatgagc tgcagaagga caagatggcc gaggcctact ccgagatcgg catgaaggga      480

gagcggagaa ggggcaaggg acacgatggc ctgtatcagg gcctgagcac cgccacaaag      540

gacacctacg atgcactgca catgcaggcc ctgccaccta gaggatctgg agccacaaac      600

ttcagcctgc tgaagcaggc cggcgatgtg gaggagaatc ctggacca                   648


<210>  96
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - polynucleotide encoding TGF beta DNpart of vector 
       construct

<400>  96
atgggaagag gactgctgag gggactgtgg ccactgcaca tcgtgctgtg gaccaggatc       60

gcctctacaa tcccacccca cgtgcagaag agcgtgaaca atgacatgat cgtgaccgat      120

aacaatggcg ccgtgaagtt tccccagctg tgcaagttct gtgacgtgcg cttttccacc      180

tgtgataacc agaagtcctg catgtctaat tgtagcatca catccatctg cgagaagcct      240

caggaggtgt gcgtggccgt gtggcggaag aacgacgaga atatcaccct ggagacagtg      300

tgccacgatc ccaagctgcc ttatcacgac ttcatcctgg aggatgccgc ctctcctaag      360

tgtatcatga aggagaagaa gaagccaggc gagaccttct ttatgtgcag ctgttcctct      420

gacgagtgca acgataatat catcttctcc gaggagtaca acacctctaa tcctgacctg      480

ctgctggtca tctttcaggt gacaggcatc tccctgctgc ctccactggg cgtggccatc      540

tctgtgatca tcatctttta ttgttacaga gtgaacaggc agcagaagcg ccggcgctag      600


<210>  97
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human Glycophorin A TM_ CT

<400>  97

Ser Gly His Phe Ser Glu Pro Glu Ile Thr Leu Ile Ile Phe Gly Val 
1               5                   10                  15      


Met Ala Gly Val Ile Gly Thr Ile Leu Leu Ile Ser Tyr Gly Ile Arg 
            20                  25                  30          


Arg Leu Ile Lys Lys Ser Pro Ser Asp Val Lys Pro Leu Pro Ser Pro 
        35                  40                  45              


Asp Thr Asp Val Pro Leu Ser Ser Val Glu Ile Glu Asn Pro Glu Thr 
    50                  55                  60                  


Ser Asp Gln 
65          


<210>  98
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  218 linker_human Glycophorin A ecto-TM_HIV Env CT

<400>  98
tccggacact tcagcgagcc tgagatcacc ctgatcatct tcggcgtgat ggccggagtg       60

atcggcacaa tcctgctgat cagctacggc atcagaagac tgattaagaa atccccatct      120

gatgtgaagc ctctgccttc tcctgacacc gacgtccccc tgagcagcgt ggaaatcgag      180

aaccccgaaa ccagcgacca g                                                201


<210>  99
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gag protein

<400>  99

Met Gly Ala Arg Ala Ser Val Leu Ser Gly Gly Glu Leu Asp Arg Trp 
1               5                   10                  15      


Glu Lys Ile Arg Leu Arg Pro Gly Gly Lys Lys Lys Tyr Lys Leu Lys 
            20                  25                  30          


His Ile Val Trp Ala Ser Arg Glu Leu Glu Arg Phe Ala Val Asn Pro 
        35                  40                  45              


Gly Leu Leu Glu Thr Ser Glu Gly Cys Arg Gln Ile Leu Gly Gln Leu 
    50                  55                  60                  


Gln Pro Ser Leu Gln Thr Gly Ser Glu Glu Leu Arg Ser Leu Tyr Asn 
65                  70                  75                  80  


Thr Val Ala Thr Leu Tyr Cys Val His Gln Arg Ile Glu Ile Lys Asp 
                85                  90                  95      


Thr Lys Glu Ala Leu Asp Lys Ile Glu Glu Glu Gln Asn Lys Ser Lys 
            100                 105                 110         


Lys Lys Ala Gln Gln Ala Ala Ala Asp Thr Gly His Ser Asn Gln Val 
        115                 120                 125             


Ser Gln Asn Tyr Pro Ile Val Gln Asn Ile Gln Gly Gln Met Val His 
    130                 135                 140                 


Gln Ala Ile Ser Pro Arg Thr Leu Asn Ala Trp Val Lys Val Val Glu 
145                 150                 155                 160 


Glu Lys Ala Phe Ser Pro Glu Val Ile Pro Met Phe Ser Ala Leu Ser 
                165                 170                 175     


Glu Gly Ala Thr Pro Gln Asp Leu Asn Thr Met Leu Asn Thr Val Gly 
            180                 185                 190         


Gly His Gln Ala Ala Met Gln Met Leu Lys Glu Thr Ile Asn Glu Glu 
        195                 200                 205             


Ala Ala Glu Trp Asp Arg Val His Pro Val His Ala Gly Pro Ile Ala 
    210                 215                 220                 


Pro Gly Gln Met Arg Glu Pro Arg Gly Ser Asp Ile Ala Gly Thr Thr 
225                 230                 235                 240 


Ser Thr Leu Gln Glu Gln Ile Gly Trp Met Thr His Asn Pro Pro Ile 
                245                 250                 255     


Pro Val Gly Glu Ile Tyr Lys Arg Trp Ile Ile Leu Gly Leu Asn Lys 
            260                 265                 270         


Ile Val Arg Met Tyr Ser Pro Thr Ser Ile Leu Asp Ile Arg Gln Gly 
        275                 280                 285             


Pro Lys Glu Pro Phe Arg Asp Tyr Val Asp Arg Phe Tyr Lys Thr Leu 
    290                 295                 300                 


Arg Ala Glu Gln Ala Ser Gln Glu Val Lys Asn Trp Met Thr Glu Thr 
305                 310                 315                 320 


Leu Leu Val Gln Asn Ala Asn Pro Asp Cys Lys Thr Ile Leu Lys Ala 
                325                 330                 335     


Leu Gly Pro Gly Ala Thr Leu Glu Glu Met Met Thr Ala Cys Gln Gly 
            340                 345                 350         


Val Gly Gly Pro Gly His Lys Ala Arg Val Leu Ala Glu Ala Met Ser 
        355                 360                 365             


Gln Val Thr Asn Pro Ala Thr Ile Met Ile Gln Lys Gly Asn Phe Arg 
    370                 375                 380                 


Asn Gln Arg Lys Thr Val Lys Cys Phe Asn Cys Gly Lys Glu Gly His 
385                 390                 395                 400 


Ile Ala Lys Asn Cys Arg Ala Pro Arg Lys Lys Gly Cys Trp Lys Cys 
                405                 410                 415     


Gly Lys Glu Gly His Gln Met Lys Asp Cys Thr Glu Arg Gln Ala Asn 
            420                 425                 430         


Phe Leu Gly Lys Ile Trp Pro Ser His Lys Gly Arg Pro Gly Asn Phe 
        435                 440                 445             


Leu Gln Ser Arg Pro Glu Pro Thr Ala Pro Pro Glu Glu Ser Phe Arg 
    450                 455                 460                 


Phe Gly Glu Glu Thr Thr Thr Pro Ser Gln Lys Gln Glu Pro Ile Asp 
465                 470                 475                 480 


Lys Glu Leu Tyr Pro Leu Ala Ser Leu Arg Ser Leu Phe Gly Ser Asp 
                485                 490                 495     


Pro Ser Ser Gln 
            500 


<210>  100
<211>  1003
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pol protein

<400>  100

Phe Phe Arg Glu Asp Leu Ala Phe Pro Gln Gly Lys Ala Arg Glu Phe 
1               5                   10                  15      


Ser Ser Glu Gln Thr Arg Ala Asn Ser Pro Thr Arg Arg Glu Leu Gln 
            20                  25                  30          


Val Trp Gly Arg Asp Asn Asn Ser Leu Ser Glu Ala Gly Ala Asp Arg 
        35                  40                  45              


Gln Gly Thr Val Ser Phe Ser Phe Pro Gln Ile Thr Leu Trp Gln Arg 
    50                  55                  60                  


Pro Leu Val Thr Ile Lys Ile Gly Gly Gln Leu Lys Glu Ala Leu Leu 
65                  70                  75                  80  


Asp Thr Gly Ala Asp Asp Thr Val Leu Glu Glu Met Asn Leu Pro Gly 
                85                  90                  95      


Arg Trp Lys Pro Lys Met Ile Gly Gly Ile Gly Gly Phe Ile Lys Val 
            100                 105                 110         


Arg Gln Tyr Asp Gln Ile Leu Ile Glu Ile Cys Gly His Lys Ala Ile 
        115                 120                 125             


Gly Thr Val Leu Val Gly Pro Thr Pro Val Asn Ile Ile Gly Arg Asn 
    130                 135                 140                 


Leu Leu Thr Gln Ile Gly Cys Thr Leu Asn Phe Pro Ile Ser Pro Ile 
145                 150                 155                 160 


Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met Asp Gly Pro Lys Val 
                165                 170                 175     


Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys Ala Leu Val Glu Ile 
            180                 185                 190         


Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser Lys Ile Gly Pro Glu 
        195                 200                 205             


Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys Lys Lys Asp Ser Thr 
    210                 215                 220                 


Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu Asn Lys Arg Thr Gln 
225                 230                 235                 240 


Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His Pro Ala Gly Leu Lys 
                245                 250                 255     


Gln Lys Lys Ser Val Thr Val Leu Asp Val Gly Asp Ala Tyr Phe Ser 
            260                 265                 270         


Val Pro Leu Asp Lys Asp Phe Arg Lys Tyr Thr Ala Phe Thr Ile Pro 
        275                 280                 285             


Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr Gln Tyr Asn Val Leu 
    290                 295                 300                 


Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe Gln Cys Ser Met Thr 
305                 310                 315                 320 


Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro Asp Ile Val Ile Tyr 
                325                 330                 335     


Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp Leu Glu Ile Gly Gln 
            340                 345                 350         


His Arg Thr Lys Ile Glu Glu Leu Arg Gln His Leu Leu Arg Trp Gly 
        355                 360                 365             


Phe Thr Thr Pro Asp Lys Lys His Gln Lys Glu Pro Pro Phe Leu Trp 
    370                 375                 380                 


Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr Val Gln Pro Ile Val 
385                 390                 395                 400 


Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp Ile Gln Lys Leu Val 
                405                 410                 415     


Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Ala Gly Ile Lys Val Arg 
            420                 425                 430         


Gln Leu Cys Lys Leu Leu Arg Gly Thr Lys Ala Leu Thr Glu Val Val 
        435                 440                 445             


Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala Glu Asn Arg Glu Ile 
    450                 455                 460                 


Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp Pro Ser Lys Asp Leu 
465                 470                 475                 480 


Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln Trp Thr Tyr Gln Ile 
                485                 490                 495     


Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly Lys Tyr Ala Arg Met 
            500                 505                 510         


Lys Gly Ala His Thr Asn Asp Val Lys Gln Leu Thr Glu Ala Val Gln 
        515                 520                 525             


Lys Ile Ala Thr Glu Ser Ile Val Ile Trp Gly Lys Thr Pro Lys Phe 
    530                 535                 540                 


Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Ala Trp Trp Thr Glu Tyr 
545                 550                 555                 560 


Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe Val Asn Thr Pro Pro 
                565                 570                 575     


Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu Pro Ile Ile Gly Ala 
            580                 585                 590         


Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn Arg Glu Thr Lys Leu Gly 
        595                 600                 605             


Lys Ala Gly Tyr Val Thr Asp Arg Gly Arg Gln Lys Val Val Pro Leu 
    610                 615                 620                 


Thr Asp Thr Thr Asn Gln Lys Thr Glu Leu Gln Ala Ile His Leu Ala 
625                 630                 635                 640 


Leu Gln Asp Ser Gly Leu Glu Val Asn Ile Val Thr Asp Ser Gln Tyr 
                645                 650                 655     


Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp Lys Ser Glu Ser Glu Leu 
            660                 665                 670         


Val Ser Gln Ile Ile Glu Gln Leu Ile Lys Lys Glu Lys Val Tyr Leu 
        675                 680                 685             


Ala Trp Val Pro Ala His Lys Gly Ile Gly Gly Asn Glu Gln Val Asp 
    690                 695                 700                 


Lys Leu Val Ser Ala Gly Ile Arg Lys Val Leu Phe Leu Asp Gly Ile 
705                 710                 715                 720 


Asp Lys Ala Gln Glu Glu His Glu Lys Tyr His Ser Asn Trp Arg Ala 
                725                 730                 735     


Met Ala Ser Asp Phe Asn Leu Pro Pro Val Val Ala Lys Glu Ile Val 
            740                 745                 750         


Ala Ser Cys Asp Lys Cys Gln Leu Lys Gly Glu Ala Met His Gly Gln 
        755                 760                 765             


Val Asp Cys Ser Pro Gly Ile Trp Gln Leu Asp Cys Thr His Leu Glu 
    770                 775                 780                 


Gly Lys Val Ile Leu Val Ala Val His Val Ala Ser Gly Tyr Ile Glu 
785                 790                 795                 800 


Ala Glu Val Ile Pro Ala Glu Thr Gly Gln Glu Thr Ala Tyr Phe Leu 
                805                 810                 815     


Leu Lys Leu Ala Gly Arg Trp Pro Val Lys Thr Val His Thr Asp Asn 
            820                 825                 830         


Gly Ser Asn Phe Thr Ser Thr Thr Val Lys Ala Ala Cys Trp Trp Ala 
        835                 840                 845             


Gly Ile Lys Gln Glu Phe Gly Ile Pro Tyr Asn Pro Gln Ser Gln Gly 
    850                 855                 860                 


Val Ile Glu Ser Met Asn Lys Glu Leu Lys Lys Ile Ile Gly Gln Val 
865                 870                 875                 880 


Arg Asp Gln Ala Glu His Leu Lys Thr Ala Val Gln Met Ala Val Phe 
                885                 890                 895     


Ile His Asn Phe Lys Arg Lys Gly Gly Ile Gly Gly Tyr Ser Ala Gly 
            900                 905                 910         


Glu Arg Ile Val Asp Ile Ile Ala Thr Asp Ile Gln Thr Lys Glu Leu 
        915                 920                 925             


Gln Lys Gln Ile Thr Lys Ile Gln Asn Phe Arg Val Tyr Tyr Arg Asp 
    930                 935                 940                 


Ser Arg Asp Pro Val Trp Lys Gly Pro Ala Lys Leu Leu Trp Lys Gly 
945                 950                 955                 960 


Glu Gly Ala Val Val Ile Gln Asp Asn Ser Asp Ile Lys Val Val Pro 
                965                 970                 975     


Arg Arg Lys Ala Lys Ile Ile Arg Asp Tyr Gly Lys Gln Met Ala Gly 
            980                 985                 990         


Asp Asp Cys Val Ala Ser Arg Gln  Asp Glu Asp 
        995                 1000             


<210>  101
<211>  4307
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gag-pol nucleic acid

<400>  101
atgggcgccc gcgccagcgt gctttctggc ggcgagctgg acaggtggga gaagattcgc       60

ctgcggcctg gaggaaagaa aaagtacaag ctgaagcaca tcgtgtgggc ttctcgggaa      120

ctggaaagat tcgccgtgaa ccctggactg ctagagacct ccgaaggctg cagacagatc      180

ctgggacagc tgcaacctag cctgcagacc ggcagcgagg agctgagaag cctgtacaac      240

accgtcgcca ccctgtattg tgtgcaccaa agaatcgaga tcaaggacac caaggaggct      300

ctggataaga tcgaggaaga gcagaacaag agcaagaaaa aagcccagca ggccgccgct      360

gataccggcc attctaatca ggtgtcccag aactacccca ttgtgcaaaa tatccagggc      420

cagatggtcc accaggccat cagccctaga accctgaatg cctgggtgaa ggtggtggaa      480

gagaaggcct tttctccaga ggtgatccct atgttcagcg ccctgagcga gggcgctacc      540

cctcaggacc tgaacacaat gctgaacacc gtgggcggcc accaggccgc catgcagatg      600

ctgaaagaaa ccatcaacga ggaggccgcc gaatgggacc gggtgcaccc cgttcacgcc      660

ggcccaatcg cccctggcca gatgcgggaa cctagaggca gcgacatcgc cggcacaacc      720

agcacactgc aagagcagat cggatggatg acacacaacc cccccatccc cgtgggcgaa      780

atctacaagc ggtggatcat tctgggactg aacaaaatcg ttagaatgta cagccctacc      840

agcatcctgg atatcagaca gggcccaaag gagcctttcc gggactacgt ggacagattt      900

tacaagaccc tgagagccga acaggcctcc caagaggtga agaactggat gacagagacc      960

ctgctggtgc agaatgccaa ccctgattgt aagacaatcc tgaaggccct cggacctggc     1020

gctacactgg aagaaatgat gaccgcctgc cagggcgtgg gcggccccgg ccacaaggcc     1080

agagtgctgg ccgaggctat gagccaggtg acaaaccccg ccacaatcat gatccagaag     1140

ggcaacttca gaaaccagcg gaagaccgtg aaatgcttca actgcggcaa ggaaggccac     1200

atcgcaaaga actgcagagc ccctaggaag aaaggctgtt ggaagtgcgg aaaggaagga     1260

caccaaatga aagattgtac tgagagacag gctaattttt tagggaagat ctggccttcc     1320

cacaagggaa ggccagggaa ttttcttcag agcagaccag agccaacagc cccaccagaa     1380

gagagcttca ggtttgggga agagacaaca actccctctc agaagcagga gccgatagac     1440

aaggaactgt atcctttagc ttccctcaga tcactctttg gcagcgaccc ctcgtcacaa     1500

taaagatcgg cggacagctg aaagaggcct tgctggacac cggagccgat gacaccgtgc     1560

tggaagaaat gaacctgcct ggaagatgga aacctaagat gatcggtggc atcggcggat     1620

ttatcaaagt gcgacagtat gaccagatcc tgatcgagat ttgcggccac aaagctatcg     1680

gaacagtgct ggtcggcccg acccccgtga acatcattgg ccgcaacctg ctgacacaga     1740

tcggttgtac actgaacttt cctatcagcc ctatcgaaac cgtgccggtc aagctgaagc     1800

ccggcatgga tggccctaag gtgaagcagt ggcccctgac agaggaaaag atcaaggcac     1860

tggtggaaat ctgcacagaa atggaaaaag agggcaagat ttctaaaatc ggcccagaga     1920

acccctacaa cacccctgtt ttcgccatca agaagaaaga ttccaccaag tggaggaagc     1980

tggtggactt tcgggaactg aacaagcgga cccaggattt ctgggaggtg cagctgggca     2040

tcccccaccc tgccggcctg aaacaaaaaa aaagcgtgac cgtgctggac gtgggcgacg     2100

cctatttcag cgtgcctctg gataaggact tccggaaata caccgccttt accatcccta     2160

gcatcaacaa cgagacccct ggcatccggt accagtacaa cgtgctccca cagggctgga     2220

agggctcacc cgccatcttc cagtgcagca tgaccaagat cctggagcct ttcagaaagc     2280

agaatcctga catcgtgatc taccagtaca tggacgacct gtacgtgggc tctgatctgg     2340

agatcggaca gcacagaaca aagatcgaag agctgagaca gcatctgctg agatggggtt     2400

tcaccacccc cgacaagaag caccagaagg aacctccttt tctgtggatg ggctacgagc     2460

tgcaccccga taagtggaca gtgcagccca tcgtgctgcc cgagaaggac tcctggaccg     2520

tgaacgacat tcagaagctg gtcggaaagc tgaattgggc ttcccaaatc tacgccggca     2580

tcaaggtgcg gcagctgtgc aagcttctgc gcggcacaaa ggccctgacg gaagtcgtgc     2640

cactgaccga ggaagccgaa ttagagctgg ccgaaaacag agaaattctg aaagaacctg     2700

tgcacggcgt ttactacgac ccttctaagg acctgatcgc cgaaatccag aaacaaggcc     2760

agggccagtg gacttaccaa atctaccagg agcctttcaa aaacctcaag accggcaagt     2820

acgccagaat gaagggagcc catacaaacg acgtgaagca gctgacagag gctgttcaga     2880

agatcgccac agaaagcatc gtgatctggg gcaagacccc aaagttcaag ctgcctatcc     2940

aaaaggaaac ctgggaggcc tggtggaccg agtactggca ggccacctgg attcctgaat     3000

gggagttcgt gaacacacca cctcttgtga agctgtggta ccagctggaa aaggagccaa     3060

tcatcggcgc cgagacattc tacgtggacg gcgccgccaa ccgggagacc aaactgggaa     3120

aggccggata tgtgaccgac agaggcagac aaaaggtggt gcctctgacc gatacaacta     3180

accagaaaac agagctgcag gccattcacc tggccctgca agacagcggc ctggaagtga     3240

atatcgtgac agacagtcag tacgccctgg gcatcatcca ggctcagcct gacaagagcg     3300

agagcgagct ggtgtcccag atcatcgagc agctgatcaa aaaggagaag gtttatctgg     3360

cctgggtgcc cgcccacaag ggcatcggag gcaacgagca ggtggacaag ctggtcagcg     3420

ccggcatccg gaaggtgctg ttcctggacg gcatcgacaa ggcccaggag gaacacgaga     3480

agtaccacag caactggcgg gccatggcca gcgacttcaa cctgccacct gtggttgcta     3540

aggagatcgt cgcctcttgt gataagtgcc agctgaaggg cgaggccatg cacggccaag     3600

tggattgcag ccctgggatc tggcagctag actgtaccca cctggagggc aaggtgatcc     3660

tggtggcagt gcacgtggcc agcggctaca tcgaggctga ggtgatcccc gccgaaacgg     3720

gccaggagac cgcctacttt ctgctgaagc tagccggccg gtggcctgtg aagaccgtgc     3780

acaccgataa cggcagcaat ttcaccagca caaccgtgaa ggctgcctgc tggtgggctg     3840

gaatcaagca ggagttcggc atcccataca atcctcagtc tcagggcgtg atcgagagca     3900

tgaacaagga actgaagaag atcattggtc aggtcagaga tcaggccgag cacctgaaaa     3960

ccgccgttca aatggctgtg ttcatccata acttcaaaag aaaaggcggc atcggcggct     4020

acagcgccgg cgaaagaatc gtggatatca tcgcgaccga catccaaaca aaagagctgc     4080

aaaagcaaat caccaagatc cagaacttca gagtgtacta cagagatagc agagatcctg     4140

tgtggaaggg acctgccaag ctgctgtgga agggcgaggg cgccgtggtg atccaggaca     4200

atagcgacat caaggtcgtg cccagaagaa aggctaaaat cattagagac tacggcaaac     4260

agatggccgg agatgattgc gtggcttcta gacaggacga ggactga                   4307


<210>  102
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rev protein

<400>  102

Met Ala Gly Arg Ser Gly Asp Ser Asp Glu Asp Leu Leu Lys Ala Val 
1               5                   10                  15      


Arg Leu Ile Lys Phe Leu Tyr Gln Ser Asn Pro Pro Pro Asn Pro Glu 
            20                  25                  30          


Gly Thr Arg Gln Ala Arg Arg Asn Arg Arg Arg Arg Trp Arg Glu Arg 
        35                  40                  45              


Gln Arg Gln Ile His Ser Ile Ser Glu Arg Ile Leu Ser Thr Tyr Leu 
    50                  55                  60                  


Gly Arg Ser Ala Glu Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg 
65                  70                  75                  80  


Leu Thr Leu Asp Cys Asn Glu Asp Cys Gly Thr Ser Gly Thr Gln Gly 
                85                  90                  95      


Val Gly Ser Pro Gln Ile Leu Val Glu Ser Pro Thr Ile Leu Glu Ser 
            100                 105                 110         


Gly Ala Lys Glu 
        115     


<210>  103
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rev nucleic acid

<400>  103
atggccggca gaagcggcga cagcgacgag gatctgctga aagccgtgcg gctgatcaag       60

ttcctgtacc agagcaaccc tcctcctaac cccgagggca ccagacaggc tagacggaac      120

cgcagaagaa ggtggcggga acggcaaaga cagatccact ctatcagcga gagaatcctg      180

agcacctacc tgggaagatc cgccgagcct gtccccctgc agctgcctcc actggaaaga      240

ctgaccctgg attgtaatga ggactgcggc acaagcggaa cccagggcgt gggcagcccc      300

cagattctgg tggaatcccc tacaatcctc gagtctggcg ccaaggaatg a               351


<210>  104
<211>  1539
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cocal glycoprotein

<400>  104
atgaactttc tgctgctgac cttcatcgtg ctgcctctgt gcagccacgc caagttttcc       60

atcgtgttcc cacagtccca gaagggcaac tggaagaatg tgcccagctc ctaccactat      120

tgtccttcta gctccgacca gaactggcac aatgatctgc tgggcatcac catgaaggtg      180

aagatgccta agacacacaa ggccatccag gcagatggat ggatgtgcca cgcagccaag      240

tggatcacca catgtgactt tcggtggtac ggccccaagt atatcaccca cagcatccac      300

tccatccagc ctacaagcga gcagtgcaag gagtccatca agcagaccaa gcagggcaca      360

tggatgtctc ccggcttccc ccctcagaac tgtggctacg ccaccgtgac agatagcgtg      420

gcagtggtgg tgcaggcaac cccacaccac gtgctggtgg atgagtatac aggcgagtgg      480

atcgacagcc agtttcccaa cggcaagtgc gagaccgagg agtgtgagac agtgcacaat      540

tctaccgtgt ggtacagcga ttataaggtg accggcctgt gcgacgccac actggtggat      600

accgagatca cattcttttc cgaggacggc aagaaggagt ctatcggcaa gcccaacacc      660

ggctacaggt ctaattactt cgcctatgag aagggcgata aggtgtgcaa gatgaattat      720

tgtaagcacg ccggggtgcg gctgccaagc ggcgtgtggt ttgagttcgt ggaccaggac      780

gtgtacgcag cagcaaagct gccagagtgc ccagtgggag caaccatcag cgcccccacc      840

cagacatctg tggacgtgag cctgatcctg gatgtggaga gaatcctgga ctactccctg      900

tgccaggaga catggtccaa gatccgctct aagcagcccg tgagcccagt ggacctgtct      960

tacctggcac caaagaaccc tggaacagga cctgccttta ccatcatcaa tggcacactg     1020

aagtacttcg agacccggta tatcagaatc gacatcgata acccaatcat ctccaagatg     1080

gtgggcaaga tctccggctc tcagaccgag agagagctgt ggacagagtg gttcccatac     1140

gagggcgtgg agatcggccc caatggcatc ctgaagaccc ctacaggcta taagtttcca     1200

ctgttcatga tcggccacgg catgctggac tctgatctgc acaagaccag ccaggccgag     1260

gtgtttgagc acccacacct ggcagaggca ccaaagcagc tgcccgagga ggagaccctg     1320

ttctttggcg atacaggcat ctccaagaac cctgtggagc tgatcgaggg ctggttttct     1380

agctggaagt ctaccgtggt gacattcttt ttcgccatcg gcgtgttcat cctgctgtac     1440

gtggtggcaa ggatcgtgat cgccgtgcgg tacagatatc agggcagcaa caataagaga     1500

atctataatg acatcgagat gtccaggttc cgcaagtga                            1539


<210>  105
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human Glycophorin A hinge-TM-CT

<400>  105

His Phe Ser Glu Pro Glu Ile Thr Leu Ile Ile Phe Gly Val Met Ala 
1               5                   10                  15      


Gly Val Ile Gly Thr Ile Leu Leu Ile Ser Tyr Gly Ile Arg Arg Leu 
            20                  25                  30          


Ile Lys Lys Ser Pro Ser Asp Val Lys Pro Leu Pro Ser Pro Asp Thr 
        35                  40                  45              


Asp Val Pro Leu Ser Ser Val Glu Ile Glu Asn Pro Glu Thr Ser Asp 
    50                  55                  60                  


Gln 
65  


<210>  106
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Glycophorin A hinge-TM-CT - nucleic acid

<400>  106
cacttcagcg agcctgagat caccctgatc atcttcggcg tgatggccgg agtgatcggc       60

acaatcctgc tgatcagcta cggcatcaga agactgatta agaaatcccc atctgatgtg      120

aagcctctgc cttctcctga caccgacgtc cccctgagca gcgtggaaat cgagaacccc      180

gaaaccagcg accag                                                       195


<210>  107
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_short hinge-TM-CT

<400>  107

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Gly Val Glu Leu Ile Glu Gly Trp Phe Ser 
            260                 265                 270         


Ser Trp Lys Ser Thr Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe 
        275                 280                 285             


Ile Leu Leu Tyr Val Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg 
    290                 295                 300                 


Tyr Gln Gly Ser Asn Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser 
305                 310                 315                 320 


Arg Phe Arg Lys 
                


<210>  108
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_short hinge-TM-CT - nucleic acid

<400>  108
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggag tggaactgat cgagggctgg ttcagcagct ggaaaagcac cgtggttaca      840

ttctttttcg ccatcggcgt gttcatcctg ctgtacgtgg tcgccagaat tgtgatcgcc      900

gtgcggtata gataccaggg cagcaacaac aagcggatct acaacgacat cgagatgagc      960

agattcagaa ag                                                          972


<210>  109
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_long hinge_TM_CT

<400>  109

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Ser Gly Phe Glu His Pro His Leu Ala Glu 
            260                 265                 270         


Ala Pro Lys Gln Leu Pro Glu Glu Glu Thr Leu Phe Phe Gly Asp Thr 
        275                 280                 285             


Gly Ile Ser Lys Asn Pro Val Glu Leu Ile Glu Gly Trp Phe Ser Ser 
    290                 295                 300                 


Trp Lys Ser Thr Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile 
305                 310                 315                 320 


Leu Leu Tyr Val Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr 
                325                 330                 335     


Gln Gly Ser Asn Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg 
            340                 345                 350         


Phe Arg Lys 
        355 


<210>  110
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_long hinge_TM_CT - nucleic acid

<400>  110
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggat tcgagcaccc ccacctggcc gaggccccta agcagctgcc tgaagaagag      840

acactgtttt tcggagatac cggcatcagc aaaaaccccg tggagctgat cgagggctgg      900

ttcagctctt ggaagagcac cgtggtcaca ttctttttcg ccatcggcgt ctttatcctg      960

ctgtacgtgg tagccagaat cgtgatcgcc gtgcggtaca gataccaggg cagcaacaac     1020

aagcggatct acaacgacat cgagatgagc cggttcagaa ag                        1062


<210>  111
<211>  374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ hGlycophorinA_TM_HIV Env CT

<400>  111

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Gly Gly Ser Thr Ser Gly Ser Gly Lys Pro 
            260                 265                 270         


Gly Ser Gly Glu Gly Ser Thr Lys Gly Pro Glu Ile Thr Leu Ile Ile 
        275                 280                 285             


Phe Gly Val Met Ala Gly Val Ile Gly Thr Ile Leu Leu Ile Ser Tyr 
    290                 295                 300                 


Gly Ile Arg Arg Leu Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr 
305                 310                 315                 320 


Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn Ala Thr 
                325                 330                 335     


Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val Val Gln 
            340                 345                 350         


Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg Gln Gly 
        355                 360                 365             


Leu Glu Arg Ile Leu Leu 
    370                 


<210>  112
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ hGlycophorinA_TM_HIV Env CT

<400>  112
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggag gatctacaag cggctctggc aagcctggca gcggagaagg cagcaccaag      840

ggccctgaga tcacactgat catcttcggc gtgatggccg gcgtcatcgg caccatcctg      900

ctgatcagct acggcatcag aagactggct ctgaagtact ggtggaatct gctgcaatac      960

tggagccagg agctgaaaaa cagcgccgtg tccctgctca acgccaccgc catcgccgtg     1020

gccgagggca ccgacagagt gatcgaggtg gtgcagggag cctgcagagc tattcggcac     1080

atccccagac ggatcaggca gggcctggaa agaatcctgc tg                        1122


<210>  113
<211>  380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ 218 linker_HIV Env ecto-TM-CT

<400>  113

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Gly Gly Ser Thr Ser Gly Ser Gly Lys Pro 
            260                 265                 270         


Gly Ser Gly Glu Gly Ser Thr Lys Gly Asn Trp Leu Trp Tyr Ile Arg 
        275                 280                 285             


Ile Phe Ile Ile Ile Val Gly Ser Leu Ile Gly Leu Arg Ile Val Phe 
    290                 295                 300                 


Ala Val Leu Ser Leu Val Asn Arg Gly Trp Glu Ala Leu Lys Tyr Trp 
305                 310                 315                 320 


Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val 
                325                 330                 335     


Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg 
            340                 345                 350         


Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro 
        355                 360                 365             


Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu 
    370                 375                 380 


<210>  114
<211>  1140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ 218 linker_HIV Env ecto-TM-CT - nucleic acid

<400>  114
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggag gaagcaccag cggctctggc aagcctggca gcggcgaggg ctctaccaag      840

ggcaattggc tgtggtacat cagaatcttc atcatcatcg tgggcagcct gatcggcctg      900

agaatcgtgt tcgccgtgct gagcctggtg aaccggggct gggaagctct gaagtactgg      960

tggaacctgc tgcaatactg gtcccaggag ctgaaaaaca gcgctgtgtc cctgctcaac     1020

gccaccgcca tcgccgtcgc cgagggaaca gacagagtga tcgaggtggt gcagggagcc     1080

tgcagagcca ttcggcacat ccccagacgc atcagacagg gcctggaaag aatcctgctg     1140


<210>  115
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ G4S linker_HIV Env ecto-TM-CT

<400>  115

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            260                 265                 270         


Ser Gly Gly Gly Gly Ser Tyr Ile Arg Ile Phe Ile Ile Ile Val Gly 
        275                 280                 285             


Ser Leu Ile Gly Leu Arg Ile Val Phe Ala Val Leu Ser Leu Val Asn 
    290                 295                 300                 


Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr Trp 
305                 310                 315                 320 


Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn Ala Thr Ala 
                325                 330                 335     


Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val Val Gln Gly 
            340                 345                 350         


Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg Gln Gly Leu 
        355                 360                 365             


Glu Arg Ile Leu Leu 
    370             


<210>  116
<211>  1119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_ G4S linker_HIV Env ecto-TM-CT - nucleic acid

<400>  116
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggag gcggtggagg ctctggtggc ggagggagcg gtggcggagg cagctacatc      840

agaatcttca tcatcatcgt gggcagcctg atcggcctga gaatcgtgtt cgccgttctg      900

agcctggtga accggggctg ggaagccctg aagtactggt ggaatctgct ccagtactgg      960

tctcaggagc tgaagaacag cgccgtgtcc ctgctgaacg ctacagctat cgccgtcgcc     1020

gagggcaccg acagagtgat cgaggtggtg cagggcgcct gcagagccat ccggcacatc     1080

cctagaagga ttcggcaagg cctggaaaga atcctgctg                            1119


<210>  117
<211>  856
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_short hinge_TM_CT _T2A_ Cocal envelope

<400>  117

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser Gly Val Glu Leu Ile Glu Gly Trp Phe Ser 
            260                 265                 270         


Ser Trp Lys Ser Thr Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe 
        275                 280                 285             


Ile Leu Leu Tyr Val Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg 
    290                 295                 300                 


Tyr Gln Gly Ser Asn Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser 
305                 310                 315                 320 


Arg Phe Arg Lys Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys 
                325                 330                 335     


Gly Asp Val Glu Glu Asn Pro Gly Pro Asn Phe Leu Leu Leu Thr Phe 
            340                 345                 350         


Ile Val Leu Pro Leu Cys Ser His Ala Lys Phe Ser Ile Val Phe Pro 
        355                 360                 365             


Gln Ser Gln Lys Gly Asn Trp Lys Asn Val Pro Ser Ser Tyr His Tyr 
    370                 375                 380                 


Cys Pro Ser Ser Ser Asp Gln Asn Trp His Asn Asp Leu Leu Gly Ile 
385                 390                 395                 400 


Thr Met Lys Val Lys Met Pro Lys Thr His Lys Ala Ile Gln Ala Asp 
                405                 410                 415     


Gly Trp Met Cys His Ala Ala Lys Trp Ile Thr Thr Cys Asp Phe Arg 
            420                 425                 430         


Trp Tyr Gly Pro Lys Tyr Ile Thr His Ser Ile His Ser Ile Gln Pro 
        435                 440                 445             


Thr Ser Glu Gln Cys Lys Glu Ser Ile Lys Gln Thr Lys Gln Gly Thr 
    450                 455                 460                 


Trp Met Ser Pro Gly Phe Pro Pro Gln Asn Cys Gly Tyr Ala Thr Val 
465                 470                 475                 480 


Thr Asp Ser Val Ala Val Val Val Gln Ala Thr Pro His His Val Leu 
                485                 490                 495     


Val Asp Glu Tyr Thr Gly Glu Trp Ile Asp Ser Gln Phe Pro Asn Gly 
            500                 505                 510         


Lys Cys Glu Thr Glu Glu Cys Glu Thr Val His Asn Ser Thr Val Trp 
        515                 520                 525             


Tyr Ser Asp Tyr Lys Val Thr Gly Leu Cys Asp Ala Thr Leu Val Asp 
    530                 535                 540                 


Thr Glu Ile Thr Phe Phe Ser Glu Asp Gly Lys Lys Glu Ser Ile Gly 
545                 550                 555                 560 


Lys Pro Asn Thr Gly Tyr Arg Ser Asn Tyr Phe Ala Tyr Glu Lys Gly 
                565                 570                 575     


Asp Lys Val Cys Lys Met Asn Tyr Cys Lys His Ala Gly Val Arg Leu 
            580                 585                 590         


Pro Ser Gly Val Trp Phe Glu Phe Val Asp Gln Asp Val Tyr Ala Ala 
        595                 600                 605             


Ala Lys Leu Pro Glu Cys Pro Val Gly Ala Thr Ile Ser Ala Pro Thr 
    610                 615                 620                 


Gln Thr Ser Val Asp Val Ser Leu Ile Leu Asp Val Glu Arg Ile Leu 
625                 630                 635                 640 


Asp Tyr Ser Leu Cys Gln Glu Thr Trp Ser Lys Ile Arg Ser Lys Gln 
                645                 650                 655     


Pro Val Ser Pro Val Asp Leu Ser Tyr Leu Ala Pro Lys Asn Pro Gly 
            660                 665                 670         


Thr Gly Pro Ala Phe Thr Ile Ile Asn Gly Thr Leu Lys Tyr Phe Glu 
        675                 680                 685             


Thr Arg Tyr Ile Arg Ile Asp Ile Asp Asn Pro Ile Ile Ser Lys Met 
    690                 695                 700                 


Val Gly Lys Ile Ser Gly Ser Gln Thr Glu Arg Glu Leu Trp Thr Glu 
705                 710                 715                 720 


Trp Phe Pro Tyr Glu Gly Val Glu Ile Gly Pro Asn Gly Ile Leu Lys 
                725                 730                 735     


Thr Pro Thr Gly Tyr Lys Phe Pro Leu Phe Met Ile Gly His Gly Met 
            740                 745                 750         


Leu Asp Ser Asp Leu His Lys Thr Ser Gln Ala Glu Val Phe Glu His 
        755                 760                 765             


Pro His Leu Ala Glu Ala Pro Lys Gln Leu Pro Glu Glu Glu Thr Leu 
    770                 775                 780                 


Phe Phe Gly Asp Thr Gly Ile Ser Lys Asn Pro Val Glu Leu Ile Glu 
785                 790                 795                 800 


Gly Trp Phe Ser Ser Trp Lys Ser Thr Val Val Thr Phe Phe Phe Ala 
                805                 810                 815     


Ile Gly Val Phe Ile Leu Leu Tyr Val Val Ala Arg Ile Val Ile Ala 
            820                 825                 830         


Val Arg Tyr Arg Tyr Gln Gly Ser Asn Asn Lys Arg Ile Tyr Asn Asp 
        835                 840                 845             


Ile Glu Met Ser Arg Phe Arg Lys 
    850                 855     


<210>  118
<211>  2568
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_short hinge_TM_CT _T2A_ Cocal envelope - nucleic 
       acid

<400>  118
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctccggag tggaactgat cgagggctgg ttcagcagct ggaaaagcac cgtggttaca      840

ttctttttcg ccatcggcgt gttcatcctg ctgtacgtgg tcgccagaat tgtgatcgcc      900

gtgcggtata gataccaggg cagcaacaac aagcggatct acaacgacat cgagatgagc      960

agattcagaa agggatctgg agagggaagg ggaagcctgc tgacatgcgg cgacgtggag     1020

gagaacccag gaccaaattt tctgctgctg accttcatcg tgctgcctct gtgcagccac     1080

gccaagtttt ccatcgtgtt cccacagtcc cagaagggca actggaagaa tgtgccctct     1140

agctaccact attgcccttc ctctagcgac cagaactggc acaatgatct gctgggcatc     1200

acaatgaagg tgaagatgcc caagacccac aaggccatcc aggcagatgg atggatgtgc     1260

cacgcagcca agtggatcac aacctgtgac tttcggtggt acggccccaa gtatatcaca     1320

cactccatcc actctatcca gcctacctcc gagcagtgca aggagtctat caagcagaca     1380

aagcagggca cctggatgag ccctggcttc ccaccccaga actgtggcta cgccacagtg     1440

accgactccg tggcagtggt ggtgcaggca acacctcacc acgtgctggt ggatgagtat     1500

accggcgagt ggatcgacag ccagtttcca aacggcaagt gcgagacaga ggagtgtgag     1560

accgtgcaca attctacagt gtggtacagc gattataagg tgacaggcct gtgcgacgcc     1620

accctggtgg atacagagat caccttcttt tctgaggacg gcaagaagga gagcatcggc     1680

aagcccaaca ccggctacag atccaattac ttcgcctatg agaagggcga taaggtgtgc     1740

aagatgaatt attgtaagca cgccggggtg cggctgccta gcggcgtgtg gtttgagttc     1800

gtggaccagg acgtgtacgc agcagcaaag ctgcctgagt gcccagtggg agcaaccatc     1860

tccgccccaa cacagacctc cgtggacgtg tctctgatcc tggatgtgga gcgcatcctg     1920

gactacagcc tgtgccagga gacctggagc aagatccggt ccaagcagcc cgtgtcccct     1980

gtggacctgt cttacctggc accaaagaac ccaggaaccg gaccagcctt tacaatcatc     2040

aatggcaccc tgaagtactt cgagacccgc tatatccgga tcgacatcga taaccctatc     2100

atcagcaaga tggtgggcaa gatctctggc agccagacag agagagagct gtggaccgag     2160

tggttccctt acgagggcgt ggagatcggc ccaaatggca tcctgaagac accaaccggc     2220

tataagtttc ccctgttcat gatcggccac ggcatgctgg acagcgatct gcacaagacc     2280

tcccaggccg aggtgtttga gcacccacac ctggcagagg caccaaagca gctgcctgag     2340

gaggagacac tgttctttgg cgataccggc atctctaaga accccgtgga gctgatcgag     2400

ggctggtttt cctcttggaa gagcacagtg gtgaccttct ttttcgccat cggcgtgttc     2460

atcctgctgt acgtggtggc cagaatcgtg atcgccgtga gatacaggta tcagggctcc     2520

aacaataaga ggatctataa tgacatcgag atgtctcgct tccggaag                  2568


<210>  119
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_human Glycophorin A hinge-TM-CT

<400>  119

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            20                  25                  30          


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
        35                  40                  45              


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
    50                  55                  60                  


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
                85                  90                  95      


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
            100                 105                 110         


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg Thr Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 


Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile 
            180                 185                 190         


Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys Asp Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe Leu Gln Met 
    210                 215                 220                 


Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr 
225                 230                 235                 240 


Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val 
                245                 250                 255     


Thr Val Ser Ser Ala Ser His Phe Ser Glu Pro Glu Ile Thr Leu Ile 
            260                 265                 270         


Ile Phe Gly Val Met Ala Gly Val Ile Gly Thr Ile Leu Leu Ile Ser 
        275                 280                 285             


Tyr Gly Ile Arg Arg Leu Ile Lys Lys Ser Pro Ser Asp Val Lys Pro 
    290                 295                 300                 


Leu Pro Ser Pro Asp Thr Asp Val Pro Leu Ser Ser Val Glu Ile Glu 
305                 310                 315                 320 


Asn Pro Glu Thr Ser Asp Gln 
                325         


<210>  120
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3scFv_human Glycophorin A hinge-TM-CT - nucleic acid

<400>  120
atgggcgtga aagtgctgtt cgccctgatc tgcatcgcag ttgctgaagc cgacatccag       60

atgacccagt ctcctagcag cctcagcgct agcgtgggcg atagagtgac catcacatgt      120

agcgccagca gcagcgtgtc ctacatgaac tggtaccagc aaacacctgg aaaggcccct      180

aaaaggtgga tctatgacac atctaagctg gcttctggag tgccatctag attttctggc      240

agcggctccg gcactgatta tacattcacc atcagcagcc tgcagcccga ggatatcgcc      300

acctactact gtcagcagtg gtcctctaat cccttcacct tcggccaggg caccaagctg      360

cagatcacca gaaccagcgg cgggggagga agcggcgggg gaggatctgg cggcggcggc      420

agccaggtgc agctggtgca gagcggcggc ggcgtggtgc aacctggcag aagcctgaga      480

ctgagctgca aggcctctgg ctacaccttc acccggtaca ccatgcattg ggtgcggcag      540

gcccctggca agggcctgga atggattgga tacatcaacc ccagcagagg ctacaccaac      600

tacaaccaga aggtgaagga cagattcaca atttctcggg acaacagcaa gaataccgcc      660

ttcctgcaaa tggactccct gcgcccagaa gataccggcg tgtacttctg cgctagatat      720

tacgacgacc actactgcct ggactactgg ggccagggca cccctgtgac cgtgtccagc      780

gcctcccact tcagcgagcc tgagatcacc ctgatcatct tcggcgtgat ggccggagtg      840

atcggcacaa tcctgctgat cagctacggc atcagaagac tgattaagaa atccccatct      900

gatgtgaagc ctctgccttc tcctgacacc gacgtccccc tgagcagcgt ggaaatcgag      960

aaccccgaaa ccagcgacca g                                                981


<210>  121
<211>  7145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  payload plasmid 142 - CAR comprising anti-CD19, cytosolic FRB, 
       RACR

<400>  121
tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg       60

ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc      120

gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc      180

cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt      240

tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc      300

aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta      360

actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa      420

cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc      480

tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac      540

tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat      600

tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      660

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      720

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      780

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      840

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      900

gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca      960

attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac     1020

ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt     1080

tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga     1140

cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg     1200

ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg     1260

caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt     1320

gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat     1380

ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt     1440

acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac     1500

aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt     1560

ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag     1620

tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc     1680

agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg     1740

gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa     1800

cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa     1860

tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta     1920

tcggccgcgg ggtacctagg aacagagaaa caggagaata tgggccaaac aggatatctg     1980

tggtaagcag ttcctgcccc ggctcagggc caagaacagt tggaacagca gaatatgggc     2040

caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc     2100

cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc     2160

ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct     2220

tctgttcgcg cgcttctgct ccccgagctc tatataagca gagctcgttt agtgaaccgt     2280

cagatcgcct ggagacgcca tccacgctgt tttgacttcc atagaagcct gcagggccgc     2340

caccatgctg ctgctggtga cctccctgct gctgtgcgag ctgcctcacc cagcctttct     2400

gctgatcccc gacatccaga tgacacagac cacaagctcc ctgtctgcca gcctgggcga     2460

cagagtgacc atctcctgta gggcctctca ggatatcagc aagtacctga actggtatca     2520

gcagaagcca gatggcacag tgaagctgct gatctaccac acctccaggc tgcactctgg     2580

agtgccaagc cggttctccg gatctggaag cggcaccgac tattccctga caatctctaa     2640

cctggagcag gaggatatcg ccacatactt ttgccagcag ggcaataccc tgccatatac     2700

attcggcgga ggaaccaagc tggagatcac cggatccaca tctggaagcg gcaagccagg     2760

aagcggagag ggatccacaa agggagaggt gaagctgcag gagagcggac caggactggt     2820

ggcaccatcc cagtctctga gcgtgacctg tacagtgtcc ggcgtgtctc tgcctgacta     2880

cggcgtgtcc tggatcaggc agccacctag gaagggactg gagtggctgg gcgtgatctg     2940

gggctctgag accacatact ataattctgc cctgaagagc cgcctgacca tcatcaagga     3000

caactccaag tctcaggtgt ttctgaagat gaatagcctg cagaccgacg atacagccat     3060

ctactattgc gccaagcact actattacgg cggctcctac gccatggatt attggggcca     3120

gggcacctcc gtgacagtgt ctagcgagtc taagtatggc ccaccctgcc ctccatgtcc     3180

aatgttctgg gtgctggtgg tggtgggagg cgtgctggcc tgttactccc tgctggtgac     3240

cgtggccttt atcatcttct gggtgaagag aggcaggaag aagctgctgt atatctttaa     3300

gcagcccttc atgcgccctg tgcagaccac acaggaggag gacggctgca gctgtcggtt     3360

tccagaggag gaggagggag gatgcgagct gcgcgtgaag ttcagccggt ccgccgatgc     3420

ccctgcctac cagcagggcc agaaccagct gtataacgag ctgaatctgg gccggagaga     3480

ggagtacgac gtgctggata agaggagggg aagggaccca gagatgggag gcaagcctcg     3540

gagaaagaac ccacaggagg gcctgtacaa tgagctgcag aaggacaaga tggccgaggc     3600

ctattctgag atcggcatga agggagagag gcgccggggc aagggacacg atggcctgta     3660

ccagggcctg agcaccgcca caaaggacac atatgatgcc ctgcacatgc aggccctgcc     3720

acctagggga tctggagcca ccaactttag cctgctgaag caggcaggcg atgtggagga     3780

gaatccagga cctgagatgt ggcacgaggg actggaggag gcaagcaggc tgtactttgg     3840

cgagcggaat gtgaagggca tgttcgaggt gctggagcca ctgcacgcaa tgatggagag     3900

gggaccacag accctgaagg agacatcctt caaccaggca tacggaaggg acctgatgga     3960

ggcacaggag tggtgccgga agtatatgaa gtctggcaat gtgaaggacc tgctgcaggc     4020

ctgggatctg tattaccacg tgtttagaag gatcagcaag ggctccggcg ccaccaactt     4080

ctccctgctg aagcaggccg gcgatgtgga agaaaatcca ggaccaatgc ctctgggact     4140

gctgtggctg ggactggccc tgctgggcgc cctgcacgcc caggccggcg tgcaggtgga     4200

gacaatcagc cctggcgacg gcagaacctt tccaaagagg ggccagacat gcgtggtgca     4260

ctacaccggc atgctggagg atggcaagaa gttcgactcc tctcgcgatc ggaacaagcc     4320

ctttaagttc atgctgggca agcaggaagt gatcagaggc tgggaggagg gcgtggccca     4380

gatgtctgtg ggccagaggg ccaagctgac aatcagccca gactatgcat acggagcaac     4440

cggacaccct ggaatcatcc caccacacgc cacactggtg ttcgatgtgg agctgctgaa     4500

gctgggcgag ggctctaaca ccagcaagga gaatccattt ctgttcgcac tggaggcagt     4560

ggtcatctcc gtgggctcta tgggcctgat catctccctg ctgtgcgtgt acttttggct     4620

ggagagaaca atgccaagga tccccaccct gaagaacctg gaggacctgg tgaccgagta     4680

ccacggcaat ttcagcgcct ggtccggcgt gtctaaggga ctggcagagt ccctgcagcc     4740

agattattct gagcggctgt gcctggtgag cgagatccct ccaaagggag gcgccctggg     4800

agagggacca ggagccagcc cctgcaacca gcactcccct tactgggccc ccccttgtta     4860

taccctgaag ccagagacag gctctggcgc caccaacttc agcctgctga agcaagccgg     4920

cgacgtggaa gaaaacccag gaccaatggc actgccagtg accgccctgc tgctgcctct     4980

ggccctgctg ctgcacgcag ccagacccat cctgtggcac gaaatgtggc atgaaggcct     5040

ggaggaggca agcagactgt actttggcga gagaaatgtg aaaggaatgt ttgaggtgct     5100

ggagcctctg cacgccatga tggagagggg ccctcagacc ctgaaggaga catcctttaa     5160

ccaggcctac ggcagagacc tgatggaggc ccaggagtgg tgcaggaagt atatgaagag     5220

cggaaatgtg aaagacctgc tgcaggcctg ggatctgtac taccacgtgt tccgccggat     5280

ctctaagggc aaggatacaa tcccttggct gggacacctg ctggtgggac tgagcggagc     5340

ctttggcttc atcatcctgg tgtatctgct gatcaactgc agaaatacag gcccatggct     5400

gaagaaggtg ctgaagtgta acacccctga cccatccaag ttcttttctc agctgagctc     5460

cgagcacggc ggcgatgtgc agaagtggct gtctagcccc tttccttcct ctagcttcag     5520

ccctggagga ctggcacctg agatctcccc actggaggtg ctggagaggg acaaggtgac     5580

ccagctgctg ctgcagcagg ataaggtgcc agagcccgcc tccctgtcct ctaaccacag     5640

cctgacctcc tgctttacaa atcagggcta cttctttttc cacctgccag acgcactgga     5700

gatcgaggca tgtcaggtgt atttcacata cgatccctat agcgaggagg accctgatga     5760

gggagtggcc ggcgccccaa ccggaagctc ccctcagcca ctgcagccac tgagcggaga     5820

ggacgatgca tattgtacat ttccttcccg cgacgatctg ctgctgttct ctccaagcct     5880

gctgggagga ccatctccac ccagcaccgc acctggagga tccggggcag gggaggagcg     5940

gatgcctcca tctctgcagg agagagtgcc aagggactgg gatccacagc ctctgggacc     6000

acctacccct ggagtgccag acctggtgga tttccagcca ccccctgagc tggtgctgcg     6060

ggaggcagga gaggaggtgc cagacgcagg acctagagag ggcgtgagct ttccctggtc     6120

caggccacca ggacagggag agttccgcgc cctgaacgcc cggctgcccc tgaatacaga     6180

cgcctacctg tctctgcagg agctgcaggg ccaggatcct acccacctgg tgtgacgccg     6240

gcgctagtgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc     6300

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg     6360

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc     6420

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg     6480

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg     6540

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga     6600

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct     6660

ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg     6720

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc     6780

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc     6840

cgcctgttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga     6900

aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg     6960

tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa     7020

cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct     7080

gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc     7140

tagca                                                                 7145


<210>  122
<211>  6926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  payload plasmid - 201 comprising an anti-CD19 CAR, cytosolic FRB,
       and RACR

<400>  122
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg      600

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg      660

cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt      720

gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt      780

ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga      840

ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa      900

aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc      960

gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat     1020

ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg     1080

gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc     1140

agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc     1200

atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa     1260

acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat     1320

atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta     1380

ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga     1440

ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca     1500

atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat     1560

ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag     1620

cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg     1680

atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg     1740

agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa     1800

attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa     1860

aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac     1920

ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt     1980

ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca     2040

ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa     2100

gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg     2160

tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata     2220

gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt     2280

caaaatttta tcgattgcct gacgcgtaat gaaagacccc acctgtaggt ttggcaagct     2340

aggatcaagg tcaggaacag agagacagca gaatatgggc caaacaggat atctgtggta     2400

agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac     2460

aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag     2520

atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag     2580

gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt     2640

tcgcgcgctt ctgctccccg agctctatat aagagcccac aacccctcac tcggcgcgtg     2700

aacacaattc tgcagtcgaa ggcgtaccgt cacttacgag tcggtagcct gcagggccgc     2760

caccatgctg ctgctggtga cctccctgct gctgtgcgag ctgcctcacc cagcctttct     2820

gctgatcccc gacatccaga tgacacagac cacaagctcc ctgtctgcca gcctgggcga     2880

cagagtgacc atctcctgta gggcctctca ggatatcagc aagtacctga actggtatca     2940

gcagaagcca gatggcacag tgaagctgct gatctaccac acctccaggc tgcactctgg     3000

agtgccaagc cggttctccg gatctggaag cggcaccgac tattccctga caatctctaa     3060

cctggagcag gaggatatcg ccacatactt ttgccagcag ggcaataccc tgccatatac     3120

attcggcgga ggaaccaagc tggagatcac cggatccaca tctggaagcg gcaagccagg     3180

aagcggagag ggatccacaa agggagaggt gaagctgcag gagagcggac caggactggt     3240

ggcaccatcc cagtctctga gcgtgacctg tacagtgtcc ggcgtgtctc tgcctgacta     3300

cggcgtgtcc tggatcaggc agccacctag gaagggactg gagtggctgg gcgtgatctg     3360

gggctctgag accacatact ataattctgc cctgaagagc cgcctgacca tcatcaagga     3420

caactccaag tctcaggtgt ttctgaagat gaatagcctg cagaccgacg atacagccat     3480

ctactattgc gccaagcact actattacgg cggctcctac gccatggatt attggggcca     3540

gggcacctcc gtgacagtgt ctagcgagtc taagtatggc ccaccctgcc ctccatgtcc     3600

aatgttctgg gtgctggtgg tggtgggagg cgtgctggcc tgttactccc tgctggtgac     3660

cgtggccttt atcatcttct gggtgaagag aggcaggaag aagctgctgt atatctttaa     3720

gcagcccttc atgcgccctg tgcagaccac acaggaggag gacggctgca gctgtcggtt     3780

tccagaggag gaggagggag gatgcgagct gcgcgtgaag ttcagccggt ccgccgatgc     3840

ccctgcctac cagcagggcc agaaccagct gtataacgag ctgaatctgg gccggagaga     3900

ggagtacgac gtgctggata agaggagggg aagggaccca gagatgggag gcaagcctcg     3960

gagaaagaac ccacaggagg gcctgtacaa tgagctgcag aaggacaaga tggccgaggc     4020

ctattctgag atcggcatga agggagagag gcgccggggc aagggacacg atggcctgta     4080

ccagggcctg agcaccgcca caaaggacac atatgatgcc ctgcacatgc aggccctgcc     4140

acctagggga tctggagcca ccaactttag cctgctgaag caggcaggcg atgtggagga     4200

gaatccagga cctgagatgt ggcacgaggg actggaggag gcaagcaggc tgtactttgg     4260

cgagcggaat gtgaagggca tgttcgaggt gctggagcca ctgcacgcaa tgatggagag     4320

gggaccacag accctgaagg agacatcctt caaccaggca tacggaaggg acctgatgga     4380

ggcacaggag tggtgccgga agtatatgaa gtctggcaat gtgaaggacc tgctgcaggc     4440

ctgggatctg tattaccacg tgtttagaag gatcagcaag ggctccggcg ccaccaactt     4500

ctccctgctg aagcaggccg gcgatgtgga agaaaatcca ggaccaatgc ctctgggact     4560

gctgtggctg ggactggccc tgctgggcgc cctgcacgcc caggccggcg tgcaggtgga     4620

gacaatcagc cctggcgacg gcagaacctt tccaaagagg ggccagacat gcgtggtgca     4680

ctacaccggc atgctggagg atggcaagaa gttcgactcc tctcgcgatc ggaacaagcc     4740

ctttaagttc atgctgggca agcaggaagt gatcagaggc tgggaggagg gcgtggccca     4800

gatgtctgtg ggccagaggg ccaagctgac aatcagccca gactatgcat acggagcaac     4860

cggacaccct ggaatcatcc caccacacgc cacactggtg ttcgatgtgg agctgctgaa     4920

gctgggcgag ggctctaaca ccagcaagga gaatccattt ctgttcgcac tggaggcagt     4980

ggtcatctcc gtgggctcta tgggcctgat catctccctg ctgtgcgtgt acttttggct     5040

ggagagaaca atgccaagga tccccaccct gaagaacctg gaggacctgg tgaccgagta     5100

ccacggcaat ttcagcgcct ggtccggcgt gtctaaggga ctggcagagt ccctgcagcc     5160

agattattct gagcggctgt gcctggtgag cgagatccct ccaaagggag gcgccctggg     5220

agagggacca ggagccagcc cctgcaacca gcactcccct tactgggccc ccccttgtta     5280

taccctgaag ccagagacag gctctggcgc caccaacttc agcctgctga agcaagccgg     5340

cgacgtggaa gaaaacccag gaccaatggc actgccagtg accgccctgc tgctgcctct     5400

ggccctgctg ctgcacgcag ccagacccat cctgtggcac gaaatgtggc atgaaggcct     5460

ggaggaggca agcagactgt actttggcga gagaaatgtg aaaggaatgt ttgaggtgct     5520

ggagcctctg cacgccatga tggagagggg ccctcagacc ctgaaggaga catcctttaa     5580

ccaggcctac ggcagagacc tgatggaggc ccaggagtgg tgcaggaagt atatgaagag     5640

cggaaatgtg aaagacctgc tgcaggcctg ggatctgtac taccacgtgt tccgccggat     5700

ctctaagggc aaggatacaa tcccttggct gggacacctg ctggtgggac tgagcggagc     5760

ctttggcttc atcatcctgg tgtatctgct gatcaactgc agaaatacag gcccatggct     5820

gaagaaggtg ctgaagtgta acacccctga cccatccaag ttcttttctc agctgagctc     5880

cgagcacggc ggcgatgtgc agaagtggct gtctagcccc tttccttcct ctagcttcag     5940

ccctggagga ctggcacctg agatctcccc actggaggtg ctggagaggg acaaggtgac     6000

ccagctgctg ctgcagcagg ataaggtgcc agagcccgcc tccctgtcct ctaaccacag     6060

cctgacctcc tgctttacaa atcagggcta cttctttttc cacctgccag acgcactgga     6120

gatcgaggca tgtcaggtgt atttcacata cgatccctat agcgaggagg accctgatga     6180

gggagtggcc ggcgccccaa ccggaagctc ccctcagcca ctgcagccac tgagcggaga     6240

ggacgatgca tattgtacat ttccttcccg cgacgatctg ctgctgttct ctccaagcct     6300

gctgggagga ccatctccac ccagcaccgc acctggagga tccggggcag gggaggagcg     6360

gatgcctcca tctctgcagg agagagtgcc aagggactgg gatccacagc ctctgggacc     6420

acctacccct ggagtgccag acctggtgga tttccagcca ccccctgagc tggtgctgcg     6480

ggaggcagga gaggaggtgc cagacgcagg acctagagag ggcgtgagct ttccctggtc     6540

caggccacca ggacagggag agttccgcgc cctgaacgcc cggctgcccc tgaatacaga     6600

cgcctacctg tctctgcagg agctgcaggg ccaggatcct acccacctgg tgtgacgccg     6660

gcgctagtgt cgacgtagtg gtacctttaa gaccaatgac ttacaaggca gctgtagatc     6720

ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac     6780

aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc     6840

tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc     6900

aatgtgtgtg ttggtttttt gtgtgt                                          6926


<210>  123
<211>  3020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cocal envelope plasmid - 209

<400>  123
ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc       60

gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat      120

tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc      180

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      240

caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt      300

acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      360

ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg      420

gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac      480

gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg      540

tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac      600

gccatccacg ctgttttgac ctccatagaa gacaccgggc gagctcggat cctgagaact      660

tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt      720

catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt      780

gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt      840

ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct      900

ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct      960

gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata     1020

gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat     1080

tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca     1140

gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag cacgtgagat     1200

ctgtctgaca tcctgcaggg ccgccaccat gaactttctg ctgctgacct tcatcgtgct     1260

gcctctgtgc agccacgcca agttttccat cgtgttccca cagtcccaga agggcaactg     1320

gaagaatgtg cccagctcct accactattg tccttctagc tccgaccaga actggcacaa     1380

tgatctgctg ggcatcacca tgaaggtgaa gatgcctaag acacacaagg ccatccaggc     1440

agatggatgg atgtgccacg cagccaagtg gatcaccaca tgtgactttc ggtggtacgg     1500

ccccaagtat atcacccaca gcatccactc catccagcct acaagcgagc agtgcaagga     1560

gtccatcaag cagaccaagc agggcacatg gatgtctccc ggcttccccc ctcagaactg     1620

tggctacgcc accgtgacag atagcgtggc agtggtggtg caggcaaccc cacaccacgt     1680

gctggtggat gagtatacag gcgagtggat cgacagccag tttcccaacg gcaagtgcga     1740

gaccgaggag tgtgagacag tgcacaattc taccgtgtgg tacagcgatt ataaggtgac     1800

cggcctgtgc gacgccacac tggtggatac cgagatcaca ttcttttccg aggacggcaa     1860

gaaggagtct atcggcaagc ccaacaccgg ctacaggtct aattacttcg cctatgagaa     1920

gggcgataag gtgtgcaaga tgaattattg taagcacgcc ggggtgcggc tgccaagcgg     1980

cgtgtggttt gagttcgtgg accaggacgt gtacgcagca gcaaagctgc cagagtgccc     2040

agtgggagca accatcagcg cccccaccca gacatctgtg gacgtgagcc tgatcctgga     2100

tgtggagaga atcctggact actccctgtg ccaggagaca tggtccaaga tccgctctaa     2160

gcagcccgtg agcccagtgg acctgtctta cctggcacca aagaaccctg gaacaggacc     2220

tgcctttacc atcatcaatg gcacactgaa gtacttcgag acccggtata tcagaatcga     2280

catcgataac ccaatcatct ccaagatggt gggcaagatc tccggctctc agaccgagag     2340

agagctgtgg acagagtggt tcccatacga gggcgtggag atcggcccca atggcatcct     2400

gaagacccct acaggctata agtttccact gttcatgatc ggccacggca tgctggactc     2460

tgatctgcac aagaccagcc aggccgaggt gtttgagcac ccacacctgg cagaggcacc     2520

aaagcagctg cccgaggagg agaccctgtt ctttggcgat acaggcatct ccaagaaccc     2580

tgtggagctg atcgagggct ggttttctag ctggaagtct accgtggtga cattcttttt     2640

cgccatcggc gtgttcatcc tgctgtacgt ggtggcaagg atcgtgatcg ccgtgcggta     2700

cagatatcag ggcagcaaca ataagagaat ctataatgac atcgagatgt ccaggttccg     2760

caagtgacgc cggcgtcgac gctcaacagc ctcgactgtg ccttctagtt gccagccatc     2820

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct     2880

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg     2940

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg     3000

ggatgcggtg ggctctatgg                                                 3020


<210>  124
<211>  5398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helper plasmid 300 - gag/pol

<400>  124
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc      600

agatcactag aagcttagct gcgaagttgg tcgtgaggca ctgggcaggt aagtatcaag      660

gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc      720

ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca      780

ggtgtccact cccagttcaa ttacagctct taaggctagg atccgccgcc accatgggcg      840

cccgcgccag cgtgctttct ggcggcgagc tggacaggtg ggagaagatt cgcctgcggc      900

ctggaggaaa gaaaaagtac aagctgaagc acatcgtgtg ggcttctcgg gaactggaaa      960

gattcgccgt gaaccctgga ctgctagaga cctccgaagg ctgcagacag atcctgggac     1020

agctgcaacc tagcctgcag accggcagcg aggagctgag aagcctgtac aacaccgtcg     1080

ccaccctgta ttgtgtgcac caaagaatcg agatcaagga caccaaggag gctctggata     1140

agatcgagga agagcagaac aagagcaaga aaaaagccca gcaggccgcc gctgataccg     1200

gccattctaa tcaggtgtcc cagaactacc ccattgtgca aaatatccag ggccagatgg     1260

tccaccaggc catcagccct agaaccctga atgcctgggt gaaggtggtg gaagagaagg     1320

ccttttctcc agaggtgatc cctatgttca gcgccctgag cgagggcgct acccctcagg     1380

acctgaacac aatgctgaac accgtgggcg gccaccaggc cgccatgcag atgctgaaag     1440

aaaccatcaa cgaggaggcc gccgaatggg accgggtgca ccccgttcac gccggcccaa     1500

tcgcccctgg ccagatgcgg gaacctagag gcagcgacat cgccggcaca accagcacac     1560

tgcaagagca gatcggatgg atgacacaca acccccccat ccccgtgggc gaaatctaca     1620

agcggtggat cattctggga ctgaacaaaa tcgttagaat gtacagccct accagcatcc     1680

tggatatcag acagggccca aaggagcctt tccgggacta cgtggacaga ttttacaaga     1740

ccctgagagc cgaacaggcc tcccaagagg tgaagaactg gatgacagag accctgctgg     1800

tgcagaatgc caaccctgat tgtaagacaa tcctgaaggc cctcggacct ggcgctacac     1860

tggaagaaat gatgaccgcc tgccagggcg tgggcggccc cggccacaag gccagagtgc     1920

tggccgaggc tatgagccag gtgacaaacc ccgccacaat catgatccag aagggcaact     1980

tcagaaacca gcggaagacc gtgaaatgct tcaactgcgg caaggaaggc cacatcgcaa     2040

agaactgcag agcccctagg aagaaaggct gttggaagtg cggaaaggaa ggacaccaaa     2100

tgaaagattg tactgagaga caggctaatt ttttagggaa gatctggcct tcccacaagg     2160

gaaggccagg gaattttctt cagagcagac cagagccaac agccccacca gaagagagct     2220

tcaggtttgg ggaagagaca acaactccct ctcagaagca ggagccgata gacaaggaac     2280

tgtatccttt agcttccctc agatcactct ttggcagcga cccctcgtca caataaagat     2340

cggcggacag ctgaaagagg ccttgctgga caccggagcc gatgacaccg tgctggaaga     2400

aatgaacctg cctggaagat ggaaacctaa gatgatcggt ggcatcggcg gatttatcaa     2460

agtgcgacag tatgaccaga tcctgatcga gatttgcggc cacaaagcta tcggaacagt     2520

gctggtcggc ccgacccccg tgaacatcat tggccgcaac ctgctgacac agatcggttg     2580

tacactgaac tttcctatca gccctatcga aaccgtgccg gtcaagctga agcccggcat     2640

ggatggccct aaggtgaagc agtggcccct gacagaggaa aagatcaagg cactggtgga     2700

aatctgcaca gaaatggaaa aagagggcaa gatttctaaa atcggcccag agaaccccta     2760

caacacccct gttttcgcca tcaagaagaa agattccacc aagtggagga agctggtgga     2820

ctttcgggaa ctgaacaagc ggacccagga tttctgggag gtgcagctgg gcatccccca     2880

ccctgccggc ctgaaacaaa aaaaaagcgt gaccgtgctg gacgtgggcg acgcctattt     2940

cagcgtgcct ctggataagg acttccggaa atacaccgcc tttaccatcc ctagcatcaa     3000

caacgagacc cctggcatcc ggtaccagta caacgtgctc ccacagggct ggaagggctc     3060

acccgccatc ttccagtgca gcatgaccaa gatcctggag cctttcagaa agcagaatcc     3120

tgacatcgtg atctaccagt acatggacga cctgtacgtg ggctctgatc tggagatcgg     3180

acagcacaga acaaagatcg aagagctgag acagcatctg ctgagatggg gtttcaccac     3240

ccccgacaag aagcaccaga aggaacctcc ttttctgtgg atgggctacg agctgcaccc     3300

cgataagtgg acagtgcagc ccatcgtgct gcccgagaag gactcctgga ccgtgaacga     3360

cattcagaag ctggtcggaa agctgaattg ggcttcccaa atctacgccg gcatcaaggt     3420

gcggcagctg tgcaagcttc tgcgcggcac aaaggccctg acggaagtcg tgccactgac     3480

cgaggaagcc gaattagagc tggccgaaaa cagagaaatt ctgaaagaac ctgtgcacgg     3540

cgtttactac gacccttcta aggacctgat cgccgaaatc cagaaacaag gccagggcca     3600

gtggacttac caaatctacc aggagccttt caaaaacctc aagaccggca agtacgccag     3660

aatgaaggga gcccatacaa acgacgtgaa gcagctgaca gaggctgttc agaagatcgc     3720

cacagaaagc atcgtgatct ggggcaagac cccaaagttc aagctgccta tccaaaagga     3780

aacctgggag gcctggtgga ccgagtactg gcaggccacc tggattcctg aatgggagtt     3840

cgtgaacaca ccacctcttg tgaagctgtg gtaccagctg gaaaaggagc caatcatcgg     3900

cgccgagaca ttctacgtgg acggcgccgc caaccgggag accaaactgg gaaaggccgg     3960

atatgtgacc gacagaggca gacaaaaggt ggtgcctctg accgatacaa ctaaccagaa     4020

aacagagctg caggccattc acctggccct gcaagacagc ggcctggaag tgaatatcgt     4080

gacagacagt cagtacgccc tgggcatcat ccaggctcag cctgacaaga gcgagagcga     4140

gctggtgtcc cagatcatcg agcagctgat caaaaaggag aaggtttatc tggcctgggt     4200

gcccgcccac aagggcatcg gaggcaacga gcaggtggac aagctggtca gcgccggcat     4260

ccggaaggtg ctgttcctgg acggcatcga caaggcccag gaggaacacg agaagtacca     4320

cagcaactgg cgggccatgg ccagcgactt caacctgcca cctgtggttg ctaaggagat     4380

cgtcgcctct tgtgataagt gccagctgaa gggcgaggcc atgcacggcc aagtggattg     4440

cagccctggg atctggcagc tagactgtac ccacctggag ggcaaggtga tcctggtggc     4500

agtgcacgtg gccagcggct acatcgaggc tgaggtgatc cccgccgaaa cgggccagga     4560

gaccgcctac tttctgctga agctagccgg ccggtggcct gtgaagaccg tgcacaccga     4620

taacggcagc aatttcacca gcacaaccgt gaaggctgcc tgctggtggg ctggaatcaa     4680

gcaggagttc ggcatcccat acaatcctca gtctcagggc gtgatcgaga gcatgaacaa     4740

ggaactgaag aagatcattg gtcaggtcag agatcaggcc gagcacctga aaaccgccgt     4800

tcaaatggct gtgttcatcc ataacttcaa aagaaaaggc ggcatcggcg gctacagcgc     4860

cggcgaaaga atcgtggata tcatcgcgac cgacatccaa acaaaagagc tgcaaaagca     4920

aatcaccaag atccagaact tcagagtgta ctacagagat agcagagatc ctgtgtggaa     4980

gggacctgcc aagctgctgt ggaagggcga gggcgccgtg gtgatccagg acaatagcga     5040

catcaaggtc gtgcccagaa gaaaggctaa aatcattaga gactacggca aacagatggc     5100

cggagatgat tgcgtggctt ctagacagga cgaggactga taagaattcc atgtcgacgc     5160

tcaacagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     5220

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     5280

gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc     5340

aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgg       5398


<210>  125
<211>  1153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helper plasmid 246 - Rev

<400>  125
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc      600

agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggccacca      660

tggccggcag aagcggcgac agcgacgagg atctgctgaa agccgtgcgg ctgatcaagt      720

tcctgtacca gagcaaccct cctcctaacc ccgagggcac cagacaggct agacggaacc      780

gcagaagaag gtggcgggaa cggcaaagac agatccactc tatcagcgag agaatcctga      840

gcacctacct gggaagatcc gccgagcctg tccccctgca gctgcctcca ctggaaagac      900

tgaccctgga ttgtaatgag gactgcggca caagcggaac ccagggcgtg ggcagccccc      960

agattctggt ggaatcccct acaatcctcg agtctggcgc caaggaatga taactagcac     1020

ctgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa     1080

taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg     1140

ggaggttttt taa                                                        1153


<210>  126
<211>  2454
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3 plasmid 224

<400>  126
ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc       60

gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat      120

tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc      180

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      240

caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt      300

acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      360

ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg      420

gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac      480

gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg      540

tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac      600

gccatccacg ctgttttgac ctccatagaa gacaccgggc gagctcggat cctgagaact      660

tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt      720

catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt      780

gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt      840

ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct      900

ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct      960

gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata     1020

gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat     1080

tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca     1140

gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag cacgtgagat     1200

ctgccaccat gggcgtgaaa gtgctgttcg ccctgatctg catcgcagtt gctgaagccg     1260

acatccagat gacccagtct cctagcagcc tcagcgctag cgtgggcgat agagtgacca     1320

tcacatgtag cgccagcagc agcgtgtcct acatgaactg gtaccagcaa acacctggaa     1380

aggcccctaa aaggtggatc tatgacacat ctaagctggc ttctggagtg ccatctagat     1440

tttctggcag cggctccggc actgattata cattcaccat cagcagcctg cagcccgagg     1500

atatcgccac ctactactgt cagcagtggt cctctaatcc cttcaccttc ggccagggca     1560

ccaagctgca gatcaccaga accagcggcg ggggaggaag cggcggggga ggatctggcg     1620

gcggcggcag ccaggtgcag ctggtgcaga gcggcggcgg cgtggtgcaa cctggcagaa     1680

gcctgagact gagctgcaag gcctctggct acaccttcac ccggtacacc atgcattggg     1740

tgcggcaggc ccctggcaag ggcctggaat ggattggata catcaacccc agcagaggct     1800

acaccaacta caaccagaag gtgaaggaca gattcacaat ttctcgggac aacagcaaga     1860

ataccgcctt cctgcaaatg gactccctgc gcccagaaga taccggcgtg tacttctgcg     1920

ctagatatta cgacgaccac tactgcctgg actactgggg ccagggcacc cctgtgaccg     1980

tgtccagcgc ctccggagtg gaactgatcg agggctggtt cagcagctgg aaaagcaccg     2040

tggttacatt ctttttcgcc atcggcgtgt tcatcctgct gtacgtggtc gccagaattg     2100

tgatcgccgt gcggtataga taccagggca gcaacaacaa gcggatctac aacgacatcg     2160

agatgagcag attcagaaag taatgactcg aggtgaattc gcgccggcgt cgacgctcaa     2220

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     2280

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     2340

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     2400

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgg           2454


<210>  127
<211>  2452
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD3 plasmid - 232

<400>  127
ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc       60

gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat      120

tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc      180

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      240

caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt      300

acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      360

ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg      420

gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac      480

gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg      540

tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac      600

gccatccacg ctgttttgac ctccatagaa gacaccgggc gagctcggat cctgagaact      660

tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt      720

catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt      780

gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt      840

ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct      900

ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct      960

gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata     1020

gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat     1080

tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca     1140

gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag cacgtgagat     1200

ctgccaccat gggcgtgaaa gtgctgttcg ccctgatctg catcgcagtt gctgaagccg     1260

acatccagat gacccagtct cctagcagcc tcagcgctag cgtgggcgat agagtgacca     1320

tcacatgtag cgccagcagc agcgtgtcct acatgaactg gtaccagcaa acacctggaa     1380

aggcccctaa aaggtggatc tatgacacat ctaagctggc ttctggagtg ccatctagat     1440

tttctggcag cggctccggc actgattata cattcaccat cagcagcctg cagcccgagg     1500

atatcgccac ctactactgt cagcagtggt cctctaatcc cttcaccttc ggccagggca     1560

ccaagctgca gatcaccaga accagcggcg ggggaggaag cggcggggga ggatctggcg     1620

gcggcggcag ccaggtgcag ctggtgcaga gcggcggcgg cgtggtgcaa cctggcagaa     1680

gcctgagact gagctgcaag gcctctggct acaccttcac ccggtacacc atgcattggg     1740

tgcggcaggc ccctggcaag ggcctggaat ggattggata catcaacccc agcagaggct     1800

acaccaacta caaccagaag gtgaaggaca gattcacaat ttctcgggac aacagcaaga     1860

ataccgcctt cctgcaaatg gactccctgc gcccagaaga taccggcgtg tacttctgcg     1920

ctagatatta cgacgaccac tactgcctgg actactgggg ccagggcacc cctgtgaccg     1980

tgtccagcgc ctccggacac ttcagcgagc ctgagatcac cctgatcatc ttcggcgtga     2040

tggccggagt gatcggcaca atcctgctga tcagctacgg catcagaaga ctgattaaga     2100

aatccccatc tgatgtgaag cctctgcctt ctcctgacac cgacgtcccc ctgagcagcg     2160

tggaaatcga gaaccccgaa accagcgacc agtgataaga attctagtcg acgctcaaca     2220

gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc     2280

ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg     2340

cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg     2400

gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gg             2452


<210>  128
<211>  3871
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KanR_p57M-MND-(anti)medCD3-Cocal-wPREw-BGHpA-001

<400>  128
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc      360

atccacgctg ttttgacttc catagaagcc tgcagggccg ccaccatggc actgcctgtg      420

acagccctgc tgctgccact ggccctgctg ctgcacgcag cacgcccaga tatccagatg      480

acccagtccc caagctccct gagcgcctcc gtgggcgacc gggtgacaat cacctgcagc      540

gcctctagct ccgtgtccta catgaactgg tatcagcaga cacctggcaa ggccccaaag      600

agatggatct acgataccag caagctggcc tccggcgtgc cttctaggtt ttctggcagc      660

ggctccggca cagattatac attcaccatc tctagcctgc agccagagga catcgccacc      720

tactattgcc agcagtggtc ctctaatccc tttacattcg gccagggcac caagctgcag      780

atcacaagaa cctctggagg aggaggaagc ggaggaggag gatccggcgg cggcggctct      840

caggtgcagc tggtgcagag cggaggagga gtggtgcagc caggcagaag cctgaggctg      900

tcctgtaagg cctctggcta cacattcacc agatatacaa tgcactgggt gaggcaggca      960

ccaggcaagg gactggagtg gatcggctac atcaacccct ccaggggcta caccaactat     1020

aatcagaagg tgaaggatcg gttcaccatc agcagggaca actccaagaa taccgccttc     1080

ctgcagatgg acagcctgag gccagaggat accggcgtgt acttttgcgc ccggtactat     1140

gacgatcact actgtctgga ttattggggc cagggaacac cagtgaccgt gagctccgcc     1200

gcagcaaagc ctaccacaac ccctgcccca aggccaccta cacccgcccc taccatcgcc     1260

tctcagccac tgagcctgag gccagaggca tccaggcctg ccgcaggggg ggccgtgcac     1320

acccggggcc tggactttgc ctctgatatg gcactgatcg tgctgggagg agtggcagga     1380

ctgctgctgt tcatcggact gggcatcttc ttttgcgtgc gctgtaggca ccggagaagg     1440

cagggatctg gagagggaag gggaagcctg ctgacatgcg gcgacgtgga ggagaaccca     1500

ggaccaaatt ttctgctgct gaccttcatc gtgctgcctc tgtgcagcca cgccaagttt     1560

tccatcgtgt tcccacagtc ccagaagggc aactggaaga atgtgccctc tagctaccac     1620

tattgccctt cctctagcga ccagaactgg cacaatgatc tgctgggcat cacaatgaag     1680

gtgaagatgc ccaagaccca caaggccatc caggcagatg gatggatgtg ccacgcagcc     1740

aagtggatca caacctgtga ctttcggtgg tacggcccca agtatatcac acactccatc     1800

cactctatcc agcctacctc cgagcagtgc aaggagtcta tcaagcagac aaagcagggc     1860

acctggatga gccctggctt cccaccccag aactgtggct acgccacagt gaccgactcc     1920

gtggcagtgg tggtgcaggc aacacctcac cacgtgctgg tggatgagta taccggcgag     1980

tggatcgaca gccagtttcc aaacggcaag tgcgagacag aggagtgtga gaccgtgcac     2040

aattctacag tgtggtacag cgattataag gtgacaggcc tgtgcgacgc caccctggtg     2100

gatacagaga tcaccttctt ttctgaggac ggcaagaagg agagcatcgg caagcccaac     2160

accggctaca gatccaatta cttcgcctat gagaagggcg ataaggtgtg caagatgaat     2220

tattgtaagc acgccggggt gcggctgcct agcggcgtgt ggtttgagtt cgtggaccag     2280

gacgtgtacg cagcagcaaa gctgcctgag tgcccagtgg gagcaaccat ctccgcccca     2340

acacagacct ccgtggacgt gtctctgatc ctggatgtgg agcgcatcct ggactacagc     2400

ctgtgccagg agacctggag caagatccgg tccaagcagc ccgtgtcccc tgtggacctg     2460

tcttacctgg caccaaagaa cccaggaacc ggaccagcct ttacaatcat caatggcacc     2520

ctgaagtact tcgagacccg ctatatccgg atcgacatcg ataaccctat catcagcaag     2580

atggtgggca agatctctgg cagccagaca gagagagagc tgtggaccga gtggttccct     2640

tacgagggcg tggagatcgg cccaaatggc atcctgaaga caccaaccgg ctataagttt     2700

cccctgttca tgatcggcca cggcatgctg gacagcgatc tgcacaagac ctcccaggcc     2760

gaggtgtttg agcacccaca cctggcagag gcaccaaagc agctgcctga ggaggagaca     2820

ctgttctttg gcgataccgg catctctaag aaccccgtgg agctgatcga gggctggttt     2880

tcctcttgga agagcacagt ggtgaccttc tttttcgcca tcggcgtgtt catcctgctg     2940

tacgtggtgg ccagaatcgt gatcgccgtg agatacaggt atcagggctc caacaataag     3000

aggatctata atgacatcga gatgtctcgc ttccggaagt gacgccggcg tcgacaatca     3060

acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt     3120

tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc     3180

tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc     3240

cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg     3300

gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc     3360

cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg     3420

cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg     3480

tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc     3540

agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct     3600

tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtgt gccttctagt     3660

tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact     3720

cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat     3780

tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc     3840

aggcatgctg gggatgcggt gggctctatg g                                    3871


<210>  129
<211>  4525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p57M-CMV-BGi-(anti)-med-hCD3-Cocal-BG-polyA

<400>  129
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc      420

ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt      480

ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa      540

tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc      600

agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat      660

ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg      720

agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat      780

aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt      840

aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact      900

ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca      960

ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa     1020

atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac     1080

aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc     1140

caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg     1200

gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag atctgaattc     1260

tgacactcct gcagggccgc caccatggca ctgcctgtga cagccctgct gctgccactg     1320

gccctgctgc tgcacgcagc acgcccagat atccagatga cccagtcccc aagctccctg     1380

agcgcctccg tgggcgaccg ggtgacaatc acctgcagcg cctctagctc cgtgtcctac     1440

atgaactggt atcagcagac acctggcaag gccccaaaga gatggatcta cgataccagc     1500

aagctggcct ccggcgtgcc ttctaggttt tctggcagcg gctccggcac agattataca     1560

ttcaccatct ctagcctgca gccagaggac atcgccacct actattgcca gcagtggtcc     1620

tctaatccct ttacattcgg ccagggcacc aagctgcaga tcacaagaac ctctggagga     1680

ggaggaagcg gaggaggagg atccggcggc ggcggctctc aggtgcagct ggtgcagagc     1740

ggaggaggag tggtgcagcc aggcagaagc ctgaggctgt cctgtaaggc ctctggctac     1800

acattcacca gatatacaat gcactgggtg aggcaggcac caggcaaggg actggagtgg     1860

atcggctaca tcaacccctc caggggctac accaactata atcagaaggt gaaggatcgg     1920

ttcaccatca gcagggacaa ctccaagaat accgccttcc tgcagatgga cagcctgagg     1980

ccagaggata ccggcgtgta cttttgcgcc cggtactatg acgatcacta ctgtctggat     2040

tattggggcc agggaacacc agtgaccgtg agctccgccg cagcaaagcc taccacaacc     2100

cctgccccaa ggccacctac acccgcccct accatcgcct ctcagccact gagcctgagg     2160

ccagaggcat ccaggcctgc cgcagggggg gccgtgcaca cccggggcct ggactttgcc     2220

tctgatatgg cactgatcgt gctgggagga gtggcaggac tgctgctgtt catcggactg     2280

ggcatcttct tttgcgtgcg ctgtaggcac cggagaaggc agggatctgg agagggaagg     2340

ggaagcctgc tgacatgcgg cgacgtggag gagaacccag gaccaaattt tctgctgctg     2400

accttcatcg tgctgcctct gtgcagccac gccaagtttt ccatcgtgtt cccacagtcc     2460

cagaagggca actggaagaa tgtgccctct agctaccact attgcccttc ctctagcgac     2520

cagaactggc acaatgatct gctgggcatc acaatgaagg tgaagatgcc caagacccac     2580

aaggccatcc aggcagatgg atggatgtgc cacgcagcca agtggatcac aacctgtgac     2640

tttcggtggt acggccccaa gtatatcaca cactccatcc actctatcca gcctacctcc     2700

gagcagtgca aggagtctat caagcagaca aagcagggca cctggatgag ccctggcttc     2760

ccaccccaga actgtggcta cgccacagtg accgactccg tggcagtggt ggtgcaggca     2820

acacctcacc acgtgctggt ggatgagtat accggcgagt ggatcgacag ccagtttcca     2880

aacggcaagt gcgagacaga ggagtgtgag accgtgcaca attctacagt gtggtacagc     2940

gattataagg tgacaggcct gtgcgacgcc accctggtgg atacagagat caccttcttt     3000

tctgaggacg gcaagaagga gagcatcggc aagcccaaca ccggctacag atccaattac     3060

ttcgcctatg agaagggcga taaggtgtgc aagatgaatt attgtaagca cgccggggtg     3120

cggctgccta gcggcgtgtg gtttgagttc gtggaccagg acgtgtacgc agcagcaaag     3180

ctgcctgagt gcccagtggg agcaaccatc tccgccccaa cacagacctc cgtggacgtg     3240

tctctgatcc tggatgtgga gcgcatcctg gactacagcc tgtgccagga gacctggagc     3300

aagatccggt ccaagcagcc cgtgtcccct gtggacctgt cttacctggc accaaagaac     3360

ccaggaaccg gaccagcctt tacaatcatc aatggcaccc tgaagtactt cgagacccgc     3420

tatatccgga tcgacatcga taaccctatc atcagcaaga tggtgggcaa gatctctggc     3480

agccagacag agagagagct gtggaccgag tggttccctt acgagggcgt ggagatcggc     3540

ccaaatggca tcctgaagac accaaccggc tataagtttc ccctgttcat gatcggccac     3600

ggcatgctgg acagcgatct gcacaagacc tcccaggccg aggtgtttga gcacccacac     3660

ctggcagagg caccaaagca gctgcctgag gaggagacac tgttctttgg cgataccggc     3720

atctctaaga accccgtgga gctgatcgag ggctggtttt cctcttggaa gagcacagtg     3780

gtgaccttct ttttcgccat cggcgtgttc atcctgctgt acgtggtggc cagaatcgtg     3840

atcgccgtga gatacaggta tcagggctcc aacaataaga ggatctataa tgacatcgag     3900

atgtctcgct tccggaagtg acgccggcgc tcaaatcctg cacaacagat tcttcatgtt     3960

tggaccaaat caacttgtga taccatgctc aaagaggcct caattatatt tgagttttta     4020

atttttatga aaaaaaaaaa aaaaaacgga attcacccca ccagtgcagg ctgcctatca     4080

gaaagtggtg gctggtgtgg ctaatgccct ggcccacaag tatcactaag ctcgctttct     4140

tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact aaactggggg     4200

atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt attttcattg     4260

caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg ggaggtcagt     4320

gcatttaaaa cataaagaaa tgaagagcta gttcaaacct tgggaaaata cactatatct     4380

taaactccat gaaagaaggt gaggctgcaa acagctaatg cacattggca acagcccctg     4440

atgcctatgc cttattcatc cctcagaaaa ggattcaagt agaggcttga tttggaggtt     4500

aaagttttgc taatgctgta tttta                                           4525


<210>  130
<211>  2773
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cocal envelope plasmid - 143

<400>  130
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc      360

atccacgctg ttttgacttc catagaagcc tgcagggccg ccaccatgaa ctttctgctg      420

ctgaccttca tcgtgctgcc tctgtgcagc cacgccaagt tttccatcgt gttcccacag      480

tcccagaagg gcaactggaa gaatgtgccc agctcctacc actattgtcc ttctagctcc      540

gaccagaact ggcacaatga tctgctgggc atcaccatga aggtgaagat gcctaagaca      600

cacaaggcca tccaggcaga tggatggatg tgccacgcag ccaagtggat caccacatgt      660

gactttcggt ggtacggccc caagtatatc acccacagca tccactccat ccagcctaca      720

agcgagcagt gcaaggagtc catcaagcag accaagcagg gcacatggat gtctcccggc      780

ttcccccctc agaactgtgg ctacgccacc gtgacagata gcgtggcagt ggtggtgcag      840

gcaaccccac accacgtgct ggtggatgag tatacaggcg agtggatcga cagccagttt      900

cccaacggca agtgcgagac cgaggagtgt gagacagtgc acaattctac cgtgtggtac      960

agcgattata aggtgaccgg cctgtgcgac gccacactgg tggataccga gatcacattc     1020

ttttccgagg acggcaagaa ggagtctatc ggcaagccca acaccggcta caggtctaat     1080

tacttcgcct atgagaaggg cgataaggtg tgcaagatga attattgtaa gcacgccggg     1140

gtgcggctgc caagcggcgt gtggtttgag ttcgtggacc aggacgtgta cgcagcagca     1200

aagctgccag agtgcccagt gggagcaacc atcagcgccc ccacccagac atctgtggac     1260

gtgagcctga tcctggatgt ggagagaatc ctggactact ccctgtgcca ggagacatgg     1320

tccaagatcc gctctaagca gcccgtgagc ccagtggacc tgtcttacct ggcaccaaag     1380

aaccctggaa caggacctgc ctttaccatc atcaatggca cactgaagta cttcgagacc     1440

cggtatatca gaatcgacat cgataaccca atcatctcca agatggtggg caagatctcc     1500

ggctctcaga ccgagagaga gctgtggaca gagtggttcc catacgaggg cgtggagatc     1560

ggccccaatg gcatcctgaa gacccctaca ggctataagt ttccactgtt catgatcggc     1620

cacggcatgc tggactctga tctgcacaag accagccagg ccgaggtgtt tgagcaccca     1680

cacctggcag aggcaccaaa gcagctgccc gaggaggaga ccctgttctt tggcgataca     1740

ggcatctcca agaaccctgt ggagctgatc gagggctggt tttctagctg gaagtctacc     1800

gtggtgacat tctttttcgc catcggcgtg ttcatcctgc tgtacgtggt ggcaaggatc     1860

gtgatcgccg tgcggtacag atatcagggc agcaacaata agagaatcta taatgacatc     1920

gagatgtcca ggttccgcaa gtgacgccgg cgtcgacaat caacctctgg attacaaaat     1980

ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc     2040

tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt     2100

gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg     2160

cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg     2220

tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc     2280

cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt     2340

gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct     2400

gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg     2460

cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg     2520

gatctccctt tgggccgcct ccccgcctgt gtgccttcta gttgccagcc atctgttgtt     2580

tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa     2640

taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg     2700

gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg     2760

gtgggctcta tgg                                                        2773


<210>  131
<211>  6468
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helper plasmid gag/pol - 89

<400>  131
ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag       60

cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc      120

caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg      180

gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca      240

tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc      300

ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt      360

attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata      420

gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt      480

ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca      540

aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg      600

tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg      660

atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag aacttcaggg      720

tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc      780

ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt      840

gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata      900

ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac      960

cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat     1020

aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct     1080

acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag     1140

tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct     1200

gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagcacgtg agatctgaat     1260

tcgagatctg ccgccgccat gggtgcgaga gcgtcagtat taagcggggg agaattagat     1320

cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata     1380

gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca     1440

gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa     1500

cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata     1560

aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaaaaaa     1620

gcacagcaag cagcagctga cacaggacac agcaatcagg tcagccaaaa ttaccctata     1680

gtgcagaaca tccaggggca aatggtacat caggccatat cacctagaac tttaaatgca     1740

tgggtaaaag tagtagaaga gaaggctttc agcccagaag tgatacccat gttttcagca     1800

ttatcagaag gagccacccc acaagattta aacaccatgc taaacacagt ggggggacat     1860

caagcagcca tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga atgggataga     1920

gtgcatccag tgcatgcagg gcctattgca ccaggccaga tgagagaacc aaggggaagt     1980

gacatagcag gaactactag tacccttcag gaacaaatag gatggatgac acataatcca     2040

cctatcccag taggagaaat ctataaaaga tggataatcc tgggattaaa taaaatagta     2100

agaatgtata gccctaccag cattctggac ataagacaag gaccaaagga accctttaga     2160

gactatgtag accgattcta taaaactcta agagccgagc aagcttcaca agaggtaaaa     2220

aattggatga cagaaacctt gttggtccaa aatgcgaacc cagattgtaa gactatttta     2280

aaagcattgg gaccaggagc gacactagaa gaaatgatga cagcatgtca gggagtgggg     2340

ggacccggcc ataaagcaag agttttggct gaagcaatga gccaagtaac aaatccagct     2400

accataatga tacagaaagg caattttagg aaccaaagaa agactgttaa gtgtttcaat     2460

tgtggcaaag aagggcacat agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg     2520

aaatgtggaa aggaaggaca ccaaatgaaa gattgtactg agagacaggc taatttttta     2580

gggaagatct ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag     2640

ccaacagccc caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag     2700

aagcaggagc cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc     2760

agcgacccct cgtcacaata aagatagggg ggcaattaaa ggaagctcta ttagatacag     2820

gagcagatga tacagtatta gaagaaatga atttgccagg aagatggaaa ccaaaaatga     2880

tagggggaat tggaggtttt atcaaagtaa gacagtatga tcagatactc atagaaatct     2940

gcggacataa agctataggt acagtattag taggacctac acctgtcaac ataattggaa     3000

gaaatctgtt gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg     3060

taccagtaaa attaaagcca ggaatggatg gcccaaaagt taaacaatgg ccattgacag     3120

aagaaaaaat aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt     3180

caaaaattgg gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca     3240

gtactaaatg gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct     3300

gggaagttca attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag     3360

tactggatgt gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata     3420

ctgcatttac catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg     3480

tgcttccaca gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct     3540

tagagccttt tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt     3600

atgtaggatc tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac     3660

atctgttgag gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc     3720

tttggatggg ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag     3780

aaaaggacag ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa     3840

gtcagattta tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag     3900

cactaacaga agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg     3960

agattctaaa agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag     4020

aaatacagaa gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa     4080

atctgaaaac aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat     4140

taacagaggc agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta     4200

aatttaaatt acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag     4260

ccacctggat tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc     4320

agttagagaa agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata     4380

gggaaactaa attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc     4440

ccctaacgga cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg     4500

attcgggatt agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag     4560

cacaaccaga taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa     4620

aggaaaaagt ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag     4680

tagataaatt ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg     4740

cccaagaaga acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc     4800

taccacctgt agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg     4860

aagccatgca tggacaagta gactgtagcc caggaatatg gcagctagat tgtacacatt     4920

tagaaggaaa agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag     4980

taattccagc agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat     5040

ggccagtaaa aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg     5100

ccgcctgttg gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc     5160

aaggagtaat agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc     5220

aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa     5280

aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca     5340

tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca     5400

gggacagcag agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg     5460

cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca     5520

tcagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg     5580

attaacacat ggaattccgg agcggccgca ggagctttgt tccttgggtt cttgggagca     5640

gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg     5700

tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg     5760

ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga     5820

tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc     5880

actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac     5940

acgacctgga tggagtggga cagagaaatt aacaattaca caagcttccg cggaattcac     6000

cccaccagtg caggctgcct atcagaaagt ggtggctggt gtggctaatg ccctggccca     6060

caagtatcac taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc     6120

taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct     6180

aataaaaaac atttattttc attgcaatga tgtatttaaa ttatttctga atattttact     6240

aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga gctagttcaa     6300

accttgggaa aatacactat atcttaaact ccatgaaaga aggtgaggct gcaaacagct     6360

aatgcacatt ggcaacagcc cctgatgcct atgccttatt catccctcag aaaaggattc     6420

aagtagaggc ttgatttgga ggttaaagtt ttgctatgct gtatttta                  6468


<210>  132
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helper plasmid 84 - Rev

<400>  132
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc      240

attcaccaca ttggtgtgca cctccaagct cgagctcgtt tagtgaaccg tcagatcgcc      300

tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc      360

ccctcgaagc tagtcgatta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa      420

gacctcctca aggcagtcag actcatcaag tttctctatc aaagcaaccc acctcccaat      480

cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga      540

cagatccatt cgattagtga acggatcctt agcacttatc tgggacgatc tgcggagcct      600

gtgcctcttc agctaccacc gcttgagaga cttactcttg attgtaacga ggattgtgga      660

acttctggga cgcagggggt gggaagccct caaatattgg tggaatctcc tacaatattg      720

gagtcaggag ctaaagaata gtgctgttag cttgctcaat gccacagcta tagcagtagc      780

tgaggggaca gatagggtta tagaagtagt acaagaagct tggcactggc cgtcgtttta      840

caacgtcgtg atctgagcct gggagatctc tggctaacta gggaacccac tgcttaagcc      900

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      960

taactagaga tc                                                          972


<210>  133
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3- CDR-L1

<400>  133

Ser Ala Ser Ser Ser Val Ser Tyr Met Asn 
1               5                   10  


<210>  134
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 - CDR-L2

<400>  134

Asp Thr Ser Lys Leu Ala Ser Gly 
1               5               


<210>  135
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 - CDR-L3

<400>  135

Gln Gln Trp Ser Ser Asn Pro Phe Thr 
1               5                   


<210>  136
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 - CDR-H2

<400>  136

Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val Lys 
1               5                   10                  15      


Asp 
    


<210>  137
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 - CDR-H3

<400>  137

Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
1               5                   10  


<210>  138
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-L1

<400>  138

Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn 
1               5                   10      


<210>  139
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-L2

<400>  139

His Thr Ser Arg Leu His Ser 
1               5           


<210>  140
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-L3

<400>  140

Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   


<210>  141
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-H1

<400>  141

Asp Tyr Gly Val 
1               


<210>  142
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-H2

<400>  142

Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
1               5                   10                  15      


<210>  143
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 - CDR-H3

<400>  143

His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
1               5                   10          


<210>  144
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 - CDR-H1

<400>  144

Arg Tyr Thr Met His 
1               5   


